AR018172A1 - Metodo para inducir resistencia a patogenos en plantas; proteina que tiene actividad isocorismato sintetasa y secuencia de nucleotidos que codificadicha proteina; vector que comprende dicha secuencia nucleotidica y cepa de agrobacterium que comprende dicho vector; promotor inducible por patogeno yus - Google Patents

Metodo para inducir resistencia a patogenos en plantas; proteina que tiene actividad isocorismato sintetasa y secuencia de nucleotidos que codificadicha proteina; vector que comprende dicha secuencia nucleotidica y cepa de agrobacterium que comprende dicho vector; promotor inducible por patogeno yus

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Publication number
AR018172A1
AR018172A1 ARP990101422A ARP990101422A AR018172A1 AR 018172 A1 AR018172 A1 AR 018172A1 AR P990101422 A ARP990101422 A AR P990101422A AR P990101422 A ARP990101422 A AR P990101422A AR 018172 A1 AR018172 A1 AR 018172A1
Authority
AR
Argentina
Prior art keywords
protein
vector
plants
understanding
isocorysmate
Prior art date
Application number
ARP990101422A
Other languages
English (en)
Original Assignee
Syngenta Mogen Bv
Univ Leiden
Univ Nijmegen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Mogen Bv, Univ Leiden, Univ Nijmegen filed Critical Syngenta Mogen Bv
Publication of AR018172A1 publication Critical patent/AR018172A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un método para inducir resistencia a patogenos en plantas caracterizado porque las plantas son transformadas con un cassette de expresion que contieneun gen que codifica para la isocorismato sintetasa. Más específicamente, este método se caracterizaporque el gen que codifica se selecciona del grupo queconsiste en entC, orfA, pchA e ICS, siendo de este ultimo preferido el gen ICS de Catharantus roseus. Preferentemente el gen que codifica para la isocorismatosintetasa comprende una secuencia de nucleotidos que codifica para la proteína de secuencia SEQ ID N* 14; SEQ ID N* 16; SEQ ID N* 19. Preferentamentelas plantas son transformadas con un cassette de expresion que contiene un gen que codifica para una isocorismato piruvato liasa, preferiblemente en elmismo cassette de expresion que el gen que codifica para la isocorismato sintetasa. El gen que codifica para la isocorismato piruvato liasa espreferiblemente seleccionado del grupo que consiste en orfB y pchB. Una proteína con actividad isocorismato sintetasa aislada de Catharantus roseus. Unasecuencia de nucleotidos utilizable como promotor comprende la region reguladora 5 de la que se sabe que naturalmente regula la expresion del gen ICS enCatharantus roseus. Esta region reguladorapuede ser usada como promotor inducible por patogeno que puede conducir la expresion de una proteína quetiene propiedades antifungicas, antibacterianas o antivirales. Ejemplos de tales proteínas son quitinasas, glucanasas, osmotinas, defensinas, magaininas,cecropinas, ribozimas. Alternativamente, tal promotor inducible por patogeno puede ser usado para expresar proteínas deletéreas o genes de resistenciapara su empleo en una estrategia dirigida a la induccion de respuesta hipersensible. Un vector que comprende la secuencia nucleotídica mencionada anteriormentey cepa de Agrobacterium que comprende dicho vector. Células vegetales capaces de sobreexpresar isocorismato sintetasa y plantas que comprenden dichascélulas vegetales se transforman con los genes mencionados arriba.
ARP990101422A 1998-03-31 1999-03-30 Metodo para inducir resistencia a patogenos en plantas; proteina que tiene actividad isocorismato sintetasa y secuencia de nucleotidos que codificadicha proteina; vector que comprende dicha secuencia nucleotidica y cepa de agrobacterium que comprende dicho vector; promotor inducible por patogeno yus AR018172A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8020398P 1998-03-31 1998-03-31
US8062598P 1998-04-03 1998-04-03

Publications (1)

Publication Number Publication Date
AR018172A1 true AR018172A1 (es) 2001-10-31

Family

ID=26763209

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990101422A AR018172A1 (es) 1998-03-31 1999-03-30 Metodo para inducir resistencia a patogenos en plantas; proteina que tiene actividad isocorismato sintetasa y secuencia de nucleotidos que codificadicha proteina; vector que comprende dicha secuencia nucleotidica y cepa de agrobacterium que comprende dicho vector; promotor inducible por patogeno yus

Country Status (13)

Country Link
EP (1) EP1066389A2 (es)
JP (1) JP2003513608A (es)
CN (1) CN1295619A (es)
AR (1) AR018172A1 (es)
AU (1) AU746787B2 (es)
BR (1) BR9909303A (es)
CA (1) CA2333433A1 (es)
CO (1) CO5050251A1 (es)
MA (1) MA24793A1 (es)
MX (1) MXPA00009573A (es)
PE (1) PE20000358A1 (es)
PL (1) PL343635A1 (es)
WO (1) WO1999050423A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002006447A2 (en) * 2000-07-18 2002-01-24 The General Hospital Corporation Salicylic acid biosynthetic genes and uses thereof
CA2436528A1 (en) 2001-01-29 2002-08-08 Cargill Incorporated Fungal resistant transgenic plants
CN100412199C (zh) * 2006-04-29 2008-08-20 北京未名凯拓作物设计中心有限公司 一种提高植物中水杨酸含量的方法及其专用载体
AU2013264459A1 (en) 2012-05-25 2014-11-20 Stichting Voor De Technische Wetenschappen New plant resistance gene
CN103602693B (zh) * 2013-11-22 2017-02-01 西南大学 决明SoICS基因、用于克隆决明SoICS基因的引物及其克隆方法
CN107636147B (zh) * 2015-05-27 2021-09-10 朗泽科技新西兰有限公司 用于制造分支酸衍生物的基因工程化微生物
CN110150137B (zh) * 2019-06-03 2020-12-29 中国农业科学院植物保护研究所 一种拟南芥nbr1/atg8f双突变体的培育方法及应用
CN111440794B (zh) * 2020-04-03 2022-09-09 河南科技学院 黄瓜基因在提高光合作用、促进植株生长和自毒作用抗性中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614395A (en) * 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
ATE241699T1 (de) * 1989-03-24 2003-06-15 Syngenta Participations Ag Krankheitsresistente transgene pflanze

Also Published As

Publication number Publication date
AU746787B2 (en) 2002-05-02
JP2003513608A (ja) 2003-04-15
MXPA00009573A (es) 2003-04-22
CO5050251A1 (es) 2001-06-27
MA24793A1 (fr) 1999-10-01
PL343635A1 (en) 2001-08-27
EP1066389A2 (en) 2001-01-10
BR9909303A (pt) 2000-11-21
CA2333433A1 (en) 1999-10-07
AU3602599A (en) 1999-10-18
PE20000358A1 (es) 2000-04-27
WO1999050423A3 (en) 1999-12-16
WO1999050423A2 (en) 1999-10-07
CN1295619A (zh) 2001-05-16

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