AP819A

AP819A - Quinoline and quinazoline compounds useful in therapy.

Info

Publication number: AP819A
Application number: APAP/P/1998/001175A
Authority: AP
Inventors: David Nathan Abraham Fox
Original assignee: Pfizer
Priority date: 1997-01-11
Filing date: 1998-01-06
Publication date: 2000-04-03
Also published as: US6365599B1; SA97180705B1; IL130762A0; CN1093858C; IS5093A; BR9807068A; CN1403453A; IL130762A; PA8444301A1; GB9700504D0; DK0968208T3; HK1054389A1; NO993396L; US20030130259A1; US6521629B2; BG103560A; ID21863A; EP0968208B1; PT968208E; HK1025327A1

Abstract

Compounds of formula I, wherein R' represents CM alkoxy optionally substituted by one or more fluorine atoms; R: represents H or C16 alkoxy optionally substituted by one or more fluorine atoms; R' represents a 5- or 6-membered heterocyclic ring, the ring being optionally substituted; Ra represents a 4-, 5-, 6-, or 7-membered heterocyclic ring, the ring being optionally fused to a benzene ring or a 5- or 6-membered heterocyclic ring, the ring system as a whole being optionally substituted; X represents CH or N; and L is absent. or represents a cyclic group of formula la, and pharmaceutically acceptable salts thereof; are useful in therapy, in particular in the treatment of benign prostatic hyperplasia.

Description

Quinoline and quinazoline compounds useful in therapy

This invention relates to novel compounds useful in therapy, particularly in the treatment of benign prostatic hyperplasia.

International Patent Application WO 89/05297 discloses a number of substituted quinazoline compounds which are indicated as inhibitors of gastric acid secretion.

International Patent Application WO 97/23462 (published after the priority date of this 10 application) discloses quinoline and quinazoline compounds having a 5-phenvI substituent.

The compounds are indicated in the treatment of benign prostatic hyperplasia.

According to the present invention, there is provided a compound of formula I,

wherein

R¹ represents C,_, alkoxy optionally substituted by one or more fluorine atoms;

R² represents H or C,_₆ alkoxy optionally substituted by one or more fluorine atoms;

R³ represents a 5- or 6-membered heterocyclic ring containing at least one heteroatom selected from Ν, O and S, the ring being optionally substituted by one or more groups selected from halogen, C_M alkoxy, C_M alkyl and CF₃;

R⁴ represents a 4-, 5-, 6-, or 7-membered heterocyclic ring containing at least one heteroatom selected from Ν, O and S, the ring being optionally fused to a benzene ring or a 5- or 6-membered heterocyclic ring containing at least one heteroatom selected from Ν, O and S, the ring system as a whole being optionally substituted by one or more groups independently selected from OH, C_M alkyl, C_M alkoxy, halogen, CONR^SR⁹, SO2NR⁸R⁹, (CH2)_bNR⁸R⁹ and NHSO,(C_M alkyl), and when S is a member of the ring system, it may be substituted by one or two oxygen atoms;

R^s and R⁹ independently represent H or C,_, alkyl, or together with the N atom to which they are attached they may represent a 5- or 6-membered hpiewwcl'ic iiilg TOntamingat least one heteroatom selected from Ν, O and S; j NWKiP I

AP/P/ 9 8/01175

-8 JAN 199 ro. ka ty ’

AP 0 0 0 8 1 9 b represents 0, 1, 2 or 3;

X represents CH or N; and

L is absent, or represents a cyclic group of formula Ia, (CH₂)„ / \ la (CH₂)_m A in which N is attached to the 2-position of the quinoline or quinazoline ring;

A is absent or represents CO or SO₂;

Z represents CH or N;

m represents 1 or 2, and in addition, when Z represents CH, it may represent 0; and n represents 1, 2 or 3, provided that the sum of m and n is 2, 3, 4 or 5; or represents a chain of formula lb,

R⁶ /^N\ (CH.

in which N is attached to the 2-position of the quinoline or quinazoline ring;

A’ and Z’ have the same significance as A and Z above, respectively;

R⁶ and R⁷ independently represent H or C_M alkyl; and p represents 1, 2 or 3, and in addition, when Z’ represents CH, it may represent

0;

or a pharmaceutically acceptable salt thereof (referred to together herein as “the compounds of the invention”).

S L I I 0 / 8 6 Zd/dV

Pharmaceutically acceptable salts include acid addition salts, such as hydrochloride and hydrobromide salts, and phosphate salts.

Alkyl and alkoxy groups that R^M may represent or include can be straight chain, branched chain, cyclic, or a combination thereof.

Preferably, R³ is an aromatic ring, for example pyridinyl, pyrimidinyl, thienyl, furanyl or oxazolyl.

AP Ο Ο Ο 8 1 9

J

Heterocyclic groups that R⁴ comprises may be saturated or unsaturated. However, it is preferred that the ring attached to L, or when L is absent, to the quinoline or quinazoline ring, is saturated.

The compounds of the invention may be optically active. In particular, they may exhibit atropisomerism about the bond joining R' to the rest of the molecule when an R³ substituent is in the ortho-position of the ring. The invention includes all optical isomers of the compounds of formula I, and all diastereoisomers thereof.

Preferred groups of compounds that may be mentioned include those in which:

(a) R' represents methoxy;

(b) R² represents methoxy;

(c) R³ represents 2-pyridinyi or 2-pyrimidinyl;

(d) R⁴ comprises a saturated 6-membered N-containing ring which is fused to a • benzene or pyridine ring; for example R⁴ may be a saturated 6-membered N-containing ring which is fused to a benzene ring substituted by NHSO₂(C_M alkyl);

(e) X represents N; and (f) L is absent.

r

AP/P/ 9 8/01175

According to the invention, there is also provided a process for the production of a compound of the invention, which comprises:

(a) when X represents CH, cyclizing a compound of formula X,

in which R^M and L are as defined above;

(b) when A or A’ is present, and Z or Z’ represents N, reacting a compound of formula

XHIa-or XHIb, as appropriate.

Τι : ·*

ΑΡ Ο Ο Ο 8 1 9

XIHa

R

I

ΝΗ /

(CH₂)_p xillb in which R¹'³, R⁶, R⁷, X, m, n'and p are as defined above, with a compound of formula XIV,

..A XIV 'Lg in which R⁴ is as defined above, A” represents CO or SO₂ and Lg represents a leaving group;

(c) reacting a compound of formula XVIII, pc 'R⁴

XVIII

I nh₂ 'in which R¹, R², R⁴, X and L are as defined above, with a compound of formula XIX,

R³-M XIX in which R³ is as defined above and M represents substituted boron, zinc or tin. in the presence of a palladium catalyst;

(d) when X represents N, reacting a compound of formula XXII,

N .Cl

R NH,

XXII in which R¹'³ are as defined above, with a compound of formula XXIIIa or XXIIIb, as appropriate, (CH,) / x ₄ | Z R XXIIIa

HNR

N.

R⁴ / A' ./ (CH₂)

XXIIIb (CH₂)_m h 2'p in whi'ch R⁴, R⁶. R⁷, A, A’, Z, Z\ m, n and p are as defined above;

(e) when A or A’ represents CO and R⁴ comprises a nucleophilic nitrogen atom in the heterocyclic ring attached to L, reacting a compound of formula XXVIIIa or XXVIIIb, as appropriate, s L I 10/8« /d/dV

APO00819

in which R¹'³, R⁶, R', X. Ζ, Z’, m. n and p are as defined above, and Lg is a leaving group, with a compound of formula XXIX,

HR⁴³ XXIX in which R^4a represents the groups defined by R⁴ above which contain a nucleophilic nitrogen atom in the ring, this nucleophilic nitrogen atom being attached to H;

(f) conversion of a compound of formula I in which L represents a cyclic group of 10 formula Ia, to a corresponding compound of formula I in which L represents a chain of formula lb in which R⁶ and R' each represent H, by the action of a strong base;

(g) when A or A’ is absent and Z or Z’ represents N, reacting a compound of formula XHIa or XHIb. as defined above, with a compound of formula XXX,

R⁴-Hal XXX

AP/P/ 9 8/01 175 in which R⁴ is as defined above and Hal represents a halogen atom attached to the ring; or (h) when X represents N, L is absent and R⁴ comprises a nucleophilic nitrogen atom in the heterocyclic ring attached to the quinoline or quinazoiine ring, reacting a compound of formula XXII, as defined above, with a compound of formula XXIX, as defined above;

and where desired or necessary converting the resulting compound of formula I into a , pharmaceutically acceptable salt or vice versa.

In process (a), the cyclization may be carried out in the presence of a strong base (for 25 example lithium diisopropylamide) in a solvent which does not adversely affect the reaction (for example tetrahydrofuran) around room temperature and quenched with water.

In a variation, it may be performed using potassium hydroxide in a solvent such as DMSO at an elevated temperature. Alternatively, it may be performed using zinc chloride in a

APO 0 0 8 1 9 solvent which does not adversely affect the reaction (for example tetrahydrofuran), at the reflux temperature of the solvent.

In process (b), suitable leaving groups are OH and Cl. When the compound of formula 5 XIV is a carboxylic acid, the reaction may be carried out in the presence of conventional coupling agents [for example 1-hydroxybenzotriazole monohydrate, 1-(3dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 4-methylmorpholine] in a solvent which does not adversely affect the reaction (for example CH₂CI₂) at or around room temperature. When the leaving group is Cl, the reaction may be carried out in a solvent which does not adversely affect the reaction (for example CH₂C1₂) around 0°C.

In process (c), the palladium catalyst may be tetrakis(triphenylphosphine)palladium. M may be B(OH)₂, B(CH₂CH₂)₂, Sn(CH₂CH₂CH₂CH₃)₃ or ZnCl. The reaction may be carried out in a solvent which does not adversely affect the reaction (for example, when M is

B(OH)₂, a mixture of toluene, ethanol and IM aqueous sodium carbonate) at an elevated temperature (for example the reflux temperature of the solvent). Optionally, when M represents ZnCl or substituted Sn, copper(I) iodide may be used as a co-catalyst.

In process (d), the reaction may be carried out in a solvent which does not adversely affect the reaction (for example n-butanol) in the presence of a base (for example triethylamine) at an elevated temperature (for example 100°C).

In process (e), suitable leaving groups include Cl. The reaction may be carried out in a solvent which does not adversely affect the reaction (for example THF) in the presence of a base (for example triethylamine) at room temperature.

The reaction may also be carried out without isolating the compound of formula XXVIIIa or XXVIIIb, by reacting a compound of formula XHIa or XHIb with triphosgene and a compound of formula XXIX. In this case the leaving group is -Cl. The reaction may be carried out in a solvent which does not adversely affect the reaction (for example CH₂C1₂) in the presence of a base (for example triethylamine) at or around room temperature.

AP/P/ 9 8/01 175

AP Ο Ο Ο 8 1 9

Ί

In process (f), suitable strong bases include lithium diisopropyiamide. The reaction may be carried out in a solvent which does not adversely affect the reaction (for example THF).

In process (g), the reaction may be carried out in a solvent which does not adversely affect 5 the reaction (for example a mixture of n-BuOH and dimethylacetamide) in the presence of a base (for example triethylamine) at an elevated temperature (for example 80°C).

In process (h), the reaction may be carried out in a solvent which does not adversely affect the reaction (for example a mixture of n-butanol and dimethylacetamide) in the presence of a base (for example triethylamine) at an elevated temperature (for example 100° C).

Compounds of formula X [see process (a)] may be prepared by reaction of a compound of formula XI,

in which R^1-' are as defined above, with a combination of a compound of formula XII.

^R⁴

CH,

XII

AP/PZ 9 8/01 175

in which R⁴ and L are as defined above, and phosphorous oxychloride in dichloromethane at the reflux temperature of the solvent.

Compounds of formula Xllla or XHIb [see process (b)] in which X represents CH may be prepared from compounds of formula XVa or XVb, as appropriate,

in which R''^J, R⁶, R', m, n and p are as defined above, by bubbling HCl gas through a solution of the compound in dichloromethane.

Compounds of formula XVa or XVb may be prepared from compounds of formula XVIa or XVIb, as appropriate, o

in which R¹'³, R⁶, R⁷, m, n and p are as defined above, by cyclization using potassium hydroxide at an elevated temperature (such as 90°C) in DMSO, or lithium diisopropylamide in a solvent that does not adversely affect the reaction (for example tetrahydrofuran) around room temperature and quenching with water.

Compounds of formula XVIa or XVIb may be prepared by reacting a compound of formula XI, as defined above, with a compound of formula XVIIa or XVIIb, as appropriate, o

(CH.) A

N O Bu

XVIIa °γ^Ν

CH, (CHJ

R

I

O . N Y

CH,

R'

N ' (CH,)

XVIIb

AP/P/ 9 8/01175

2'p

O'Bu in which R⁶, R⁷, m, n and p are as defined above, by the method described above for producing compounds of formula X.

Compounds of formula XHIa or XHIb in which X represents N may be prepared by reacting a compound of formula XXII,

XXII

in which R¹ ³ are as defined above, with a compound of formula XXIIa or XXIIb, as appropriate,

ΑΡ Ο Ο Ο 8 1 9 (CHJ

ΝΗ

XXIla

ΗΝ (CH₂)„

R

I

HNR

I

NH

XXIIb (CH₂)_p in which R⁶, R⁷, m, n and p are as defined above, using the conditions mentioned for process (d) above.

Compounds of formula XVIII [see process (c)] in which X represents CH may be prepared by cyclization of a compound of formula XX,

xx in which R¹, R², R⁴ and L are as defined above, using the reaction conditions mentioned in process (a) above.

Compounds of formula XX may be prepared by reacting a compound of formula XXI,

NH,

XXI

AP/P/ 9 8/01 175 in which R¹ and R² are as defined above, with a compound of formula XII as defined above, using the method described above for the preparation of compounds of formula X.

Compounds of formula XVIII in which X represents N may be prepared by reacting a compound of formula XXVII,

XXVII

N. ,CI if ,N

I nh₂ in which R¹ and R² are as defined above, with a compound of formula XXIIIa or XXIIIb, as appropriate, as defined above, using the reaction conditions mentioned above for process (d).

APO 0 0 8 1 9

Compounds of formula XXII [see processes (d) and (h)J may be prepared from a compound of formula XXIV,

OH

XXIV in which R''^J are as defined above, by reaction with POC1₃ and N,N-dimethylaniline, followed by treatment with ammonia.

Compounds of formula XXIV may be prepared from a compound of formula XXV,

XXV in which R¹ and R² are as defined above, by reaction with a compound of formula XIX as defined above using the reaction conditions described above for process (c).

Compounds of formula XXV may be prepared from compounds of formula XXVI,

XXVI

S/110/86 /d/dV in which R¹ and R² are as defined above, using conventional techniques.

Compounds of formula XXII may also be prepared according to Scheme 1:

AP Ο Ο Ο 8 1 9

[ΧΧΐ]

ι

NH,

Pd(PPh₃)₄, Cul Τ' Ν’ dioxane I IJ [XXIIJ

1. POCIj/DMF	Rb.
2. NaOH(aq)
		I	NH, [XXVII]
	Scheme 1

Compounds of formula XXVIlla and XXVIIIb [see process (e)] in which Lg represents Cl may be prepared from compounds of formula XHIa or XHIb, as appropriate, by reaction with triphosgene. The reaction may be carried out in a solvent which does not adversely affect the reaction (for example CH₂C1₂) in the presence of a base (for example triethylamine) at around -10°C.

AP/P/ 9 8/01175

Compounds of formula X may also be prepared by reaction of a compound of formula XX with a compound of formula XIX using the conditions described for process (c).

Compounds of formulae XI, XII, XIV, XVIIa, XVIIb, XIX, XXI, XXIIa, XXIIb, XXIIIa. XXIIIb, XXVI, XXIX and XXX are either known or are available using known techniques, as illustrated by the Examples.

The intermediate compounds of formulae X, XHIa, XHIb, XXII, XXVIlla and XXVIIIb form a further aspect of the invention.

ΑΡ ο ο Ο 8 1 9

It will be apparent to those skilled in the art that sensitive functional groups may need to be protected and deprotected during synthesis of a compound of the invention. This may be achieved by conventional techniques, for example as described in 'Protective Groups in Organic Synthesis' by T W Greene and PGM Wuts, John Wiley and Sons Inc, 1991.

The compounds of the invention are useful because they possess pharmacological activity in animals. In particular, the compounds are useful in the treatment of a number of conditions including hypertension, myocardial infarction, male erectile dysfunction, hyperlipidaemia, cardiac arrhythmia and benign prostatic hyperplasia. The latter condition is of greatest interest. Thus, according to another aspect of the invention, there is provided a method of treatment of benign prostatic hyperplasia which comprises administering a therapeutically effective amount of a compound of the invention to a patient suffering from such a disorder. The use of the compounds of the invention as pharmaceuticals, and the use of the compounds of the invention in the manufacture of a medicament for the treatment of benign prostatic hyperplasia, are also provided.

The compounds of the invention may be administered by any convenient route, for example orally, parenterally (e.g. intravenously, transdermally) or rectally. The daily dose required will of course vary with the particular compound used, the particular condition being treated and with the severity of that condition. However, in general a total daily dose of from about 0.01 to lOmg/kg of body weight, and preferably about 0.05 to lmg/kg, is suitable, administered from 1 to 4 times a day. Oral administration is of particular interest.

The compounds of the invention will generally be administered in the form of a suitable pharmaceutical formulation. Thus, according to another aspect of the invention, there is provided a pharmaceutical formulation including preferably less than 50% by weight of a compound of the invention in admixture with a pharmaceutically acceptable adjuvant, diluent or carrier. The pharmaceutical formulation is preferably in unit dose form. Such forms include solid dosage forms, for example tablets, pills, capsules, powders, granules, and suppositories for oral, parenteral or rectal administration; and liquid dosage forms, for example sterile parenteral solutions or suspensions, suitably flavoured syrups, flavoured

AP/P/ 9 8/01 175

ΑΡ θ 0 0 8 1 9 'll## emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil and peanut oil, and elixirs and similar pharmaceutical vehicles.

Solid formulations may be prepared by mixing the active ingredient with pharmaceutical 5 carriers, for example conventional tabletting ingredients such as com starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate, gums and other diluents, for example water, to form a homogeneous preformulation formulation in which the active ingredient is uniformly dispersed so that it may be readily subdivided into equally effective unit dosage forms containing typically from 0.1 to about 500mg of the active ingredient. The solid dosage forms may be coated or otherwise compounded to prolong the action of the formulation.

The formulations of the invention may also contain a human 5-a reductase inhibitory compound [see International Patent Application WO 95/28397], or a compound of the invention could be presented in a pharmaceutical pack also containing a human 5-a reductase inhibitory compound as a combined preparation for simultaneous, separate or sequential use.

The compounds of the invention may be tested in the screens set out below.

Contractile responses of human prostate

Prostatic tissue was cut into longitudinal strips (approximately 3x2x10 mm) and suspended in organ baths under a resting tension of 1 g in Krebs Ringer bicarbonate 25 of the following composition (mM): NaCl (119), KC1 (4.7), CaCl₂ (2.5), KH₂PO₄ (1.2), MgSO₄ (1.2), NaHCO₃ (25), glucose (11), and gassed with 95% O₂/5% CO,. The solution also contained 10 mM cocaine and 10 mM corticosterone. Tissues were exposed to a sensitising dose of (-)-noradrenaline (100 mM) and washed over a 45 minute period. Isometric contractions were obtained in response to cumulative additions of (-)-noradrenaline to obtain control curves in all tissues. A further curve was then generated in the presence or absence of antagonist (incubated for 2 hours). Antagonist affinity estimates (pA,) were determined using a single concentration of

AP/P/ 9 8/01 175

APO Ο Ο 8 1 9 competing antagonist, pA, = -log [A]/(DR-1) where the dose ratio (DR), relative to corresponding controls, was produced by a single concentration of antagonist [A], assuming competitive antagonism and Schild regression close to unity.

Anaesthetised dog model ofprostatic pressure and blood pressure

Mature male beagles (12-15 kg body weight) were anaesthetised with sodium pentobarbitone (30-50 mg/kg i.v.) and a tracheal cannula was inserted. Subsequent anaesthesia was maintained using pentobarbitone infusion. The animals were respirated with air using a Bird Mk8 respirator (Bird Corp., Palm Springs, CA, USA) . fc adjusted to maintain blood gasses in the range pO₂ 90-110 mm Hg, pCO₂ 35-45 mm Hg, pH 7.35-7.45. Body temperature was maintained at 36-37.5°C using a heated operating table. Catheters were placed into the left femoral artery for recording blood pressure and into the left femoral vein for compound administration. Heart rate was recorded via the lead II E.C.G. A laparotomy was performed to cannulate both ureters to prevent change of fluid volume within the bladder. A 7F cardiac catheter (with a 1,5 ml capacity balloon tip) was inserted into the bladder via the urethra. The balloon was filled with air and the catheter withdrawn until the balloon _s became lodged in the prostate, which was confirmed by digital pressure. Balloon pressure was recorded via a Druck transducer. Prostatic pressure and haemodynamic parameters were made on a Grass Polygraph (Grass Instruments,

Quincy, Mass, U.S.A.) and the data measured on line using a Motorola 68000-based microcomputer system (Motorola Inc., Temple, AZ, U.S.A.). Compounds were made up in PEG 300 and administered i.v. through a catheter in the femoral vein.

Responses to phenylephrine (1-16 pg/kg i.v. in saline) were obtained to generate control dose-response curves (two control curves for each experiment). Compounds were administered (in terms of compound base) at 10-300 pg/kg i.v. 5 min before construction of phenylephrine curves (constructed up to a maximum dose of 128 .pg/kg in the presence of test compound).

Due to a|-related dysrhythymic properties of phenylephrine, absolute maximal responses were not obtained but were taken as 10 % greater than the control response

AP/P/ 9 8/01175 obtained with 16 pg/kg phenylephrine. Drug concentrations were calculated on the basis of molar weight of compound/kg body weight thus allowing a pseudo pA2 calculation by Schild analysis using dose ratios derived from shifts in the phenylephrine dose-response curves.

The compounds of the invention may have the advantage that they are more potent, have a longer duration of action, have a broader range of activity, are more stable, have fewer side effects or are more selective (in particular they may have beneficial effects in benign prostatic hyperplasia without causing undesirable cardiovascular effects, for example because they are able to selectively antagonise prostatic receptor subtypes of the a,-adrenoceptor), or have other more useful properties than the compounds of the prior art.

The invention is illustrated by the following examples, in which the following abbreviations may be used:

BuOH = butanol

DMA = dimethylacetamide DMF = dimethylformamide

DMPU = l,3-dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidone DMSO = dimethy Isulphoxide

EDTA = ethylenediaminetetraacetic acid EtOAc = ethyl acetate EtOH = ethanol h = hour

MeOH = methanol min = minute η-BuOH = n-butanol

p.s.i. = pounds per square inch

THF = tetrahydrofuran tic = thin layer chromatography

Intermediate 1

-(t-Butvloxvcarbonvl)-1.4-diazepane

AP/P/ 9 8/01175

AP Ο Ο Ο 8 1 9

To a solution of homopiperazine (lOOg, 1.0 mol) and triethylamine (210ml, 152g, 1.5mol) in CH₂C1₂ (500ml) at 0°C was added a solution of di-(t-butyl) dicarbonate (195g, 0.89mol) in CH₂C1, (300ml). The mixture was allowed to warm to room temperature and stirred for 5 18h after which time the CH₂C1₂ was evaporated under reduced pressure. The resulting residue was partitioned between ether and 2N citric acid and the aqueous layer was extracted with ether (4x200ml). The aqueous layer was basified with 2N aqueous NaOH and then extracted with CH₂C1₂ (4x400ml). The combined CH₂C1₂ extracts were washed with H₂O (2x), saturated brine (lx) and dried over MgSO₄. Evaporation under reduced 10 pressure followed by azeotroping with CH₂C1₂ (4x) gave the title compound as a yellow waxy solid (94.3g, 53%). Rf 0.25 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 201 (MH⁺). Found: C,58.86; H,10.03; N, 13.58; C_I0H₂₀N₂O₂ 0.05.CH₂Cl₂ requires C, 59.02; H,

9.91; N, 13.70%.

Intermediate 2

-(t-Butvloxycarbonvl)-4-(4-morpholinecarbonvl)-1,4-diazepane

A solution of Intermediate 1 (92.Og, 0.46mol) and triethylamine (96.0ml, 69.7g, 0.69mol) in CH₂C1₂ (500ml) at 0°C was treated dropwise with a solution of 4-morpholinecarbonyl ^X chloride (64.0ml, 82.Og, 0.55mol) in CH,C1₂ (100ml) and the reaction was stirred at room temperature under N₂ for 18h. The reaction mixture was then diluted with CH₂C1₂ (400ml) and washed with 2N citric acid (3x400ml), saturated brine (1x500ml), dried over MgSO₄and evaporated to give the title compound as an off-white solid (141.7g, 98%). Rf 0.80 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 314 (MH⁺). Found: C,57.50; H,8.69;

N,13.41; C_]5H₂₇N₃O₄ requires C, 57.50; H, 8.69; N,13.41%.

Intermediate 3 l-(4-Morpholinecarbonvl)-1.4-diazepane hydrochloride

A solution of Intermediate 2 (140.Og, 0.44mol) in CH₂Cl₂/MeOH (1/1, v/v, 600ml) at 0°C was saturated with HCI gas and the reaction mixture was jtjryji at .under j „ i

N₂ for 18h after which time the reaction mixture was ^vapf^^Rl^^^^^d pressure | V-8 JANlS?^f i 92

8/011 75

ΑΡ ο ο Ο 8 1 9 and slurried in EtOAc to give, after filtration, a white hygroscopic solid. This was further purified by slurrying in acetone, filtering, washing with ether and drying in vacuo at 60°C to give the title compound as a colourless solid (99.Og, 90%). Rf 0.41 (CH₂Cl₂/MeOH/0.88NH₃ 84/14/2, v/v). MS m/z 214 (MH). Found: C,47.50; H,8.10;

N,16.55; C₁₀H₁₉N₃O₂ HCI 0.2.H₂O requires C, 47.41; H, 8.12; N,16.59%.

Intermediate 4

-Acetvl-4-(4-morpholinecarbonyl)-1,4-diazepane

To a solution of Intermediate 3 (50g, 0.2mol) and triethylamine (42ml, 30.5g, 0.3mol) in CH₂C1₂ (400ml) at 5°C was added acetic anhydride (23ml, 24.9g, 0.24mol) dropwise over 15min and the reaction was then stirred for a further 2h at room temperature under N₂. Dilution with CH₂C1₂ (600ml) was followed by washing with saturated aqueous sodium bicarbonate (2x200ml) and the combined aqueous layers extracted with CH-.CU (lxl00ml).

The CH₂C1₂ layers were combined and washed with saturated brine, dried over MgSO₄ and evaporated to give a light brown oil. This was dissolved in CH₂C1₂ (300ml) and treated with triethylamine (8ml. 5.8g. 0.06mol) and EtOH (5ml), stirred for lh at room temperature then washed with saturated aqueous sodium bicarbonate and the aqueous layer extracted with CH₂C1₂ (5x). The combined CH,C1₂ layers were dried over MgSO₄ and evaporated under reduced pressure to give a yellow oil which was then azeotroped with CH₂C1₂ (4x) to give the title compound as a yellow oil (47.lg, 92%). Rf 0.45 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 256 (MH). Found: C,52.62; H.8.18; N,15.02; C₁₂H₂₁N₃O₃ 0.3.CH₂Cl₂ requires C,52.61; H,7.75; N,14.96%.

AP/P/ 9 8/01 175

Example 1

4-Amino-6.7-dimethoxv-2-14-(4-morpholinecarbonyl)-1.4-diazepan-l-vll-5-(thiophen-3‘ vDquinoline (a) 2-(3.4-DimethoxvphenvI)-4.4-dimethvl-A²-oxazoline 30 The subtitle compound was prepared from 3,4-dimethoxybenzoic acid according to the method of Meyers et al., J.Org.Chem., 39. 2787 (1974).

AP Ο Ο Ο 8 1 9 (b) 2-(3,4-Dimethoxy-2-iodophenvl)-4.4-dimethvl-A²-oxazoline nButyllithium (2.5M in hexane, 8.9ml, 22.3mmol) was added dropwise to a solution of the product of step (a) (4.2g, 17.8mmol) in dry ether (200ml) at 0°C and the reaction was stirred under N₂ for 2h. This was followed by the dropwise addition of iodine (5.46g,

21.5mmol) in ether (100ml) and the reaction was allowed to warm to room temperature over lh. The reaction mixture was poured onto H₂O, the ether layer was separated, washed with saturated aqueous sodium thiosulphate solution (lx) followed by saturated brine (lx) then dried over MgSO₄ and evaporated under reduced pressure to give the subtitle compound as a yellow oil (5.2g, 80%). Rf 0.60 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v).

MS m/z 362 (MH⁺).

I A (c) 3.4-Dimethoxy-2- iodo benzonitrile

To a solution of the product of step (b) (5.2g, 14.4mmol) in pyridine (30ml) was added phosphorus oxychloride (2.7ml, 4.4g, 28.8mmol) and the reaction was heated to 85°C for

18h. The reaction mixture was cooled, partitioned between saturated aqueous sodium carbonate solution (300ml) and then extracted with ether (2x100ml). The ether layer was washed with 2N HCI (2x75ml) followed by H₂O (lx) and then dried over MgSO₄ and evaporated under reduced pressure to afford a yellow oil. This was purified by slurrying with hexane and filtering to give the subtitle compound as an off-white solid (2.82g, 68%).

) 20 R, 0.80 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 307 (MH⁺). Found: C,38.03; H,2.88; N,4.64;

5¾ C₉H_sNO₂I 0.05.hexane requires C,38.05; H,2.97; N,4.77%.

s L U 0 / 8 6 'd/dV (d) 3.4-Dimethoxv-2-iodo-6-nitrobenzo nitrile

Nitronium tetrafluoroborate (1.73g, 13.0mmol) was added portionwise to a solution of the product of step (c) (2.67g, 9.2mmol) in acetonitrile (40ml) at 0°C. The reaction was stirred for 30min under N₂ and then poured into saturated aqueous sodium bicarbonate solution and extracted with EtOAc (lx). The organic layer was washed with saturated brine (lx), dried over MgSO₄ and evaporated under reduced pressure to give a residue which was slurried in hexane and filtered to give the subtitle compound as an off-white solid (2.5lg, 82%). R_f 0.46 (EtOAc/hexane 1/1, v/v). MS m/z 352 (MNHf).

p.0 BOXCYN

CAJSEWM (e)

6-Amino-3.4-dimethoxv-2-iodobenzonitrile

-8 1AN1998

APO 0 0 8 1 9

To a solution of the product of step (d) (3.50g, O.Olmol) in CH₂C1₂ (90ml) was added a solution of sodium dithionite (20.11, O.llmol) in H₂O (60ml). To the resulting mixture was added tetra-n-butylammonium chloride (1.45g, 5.24mmol) and the reaction was stirred vigorously for 1.5h. The mixture was partitioned between CH,C1₂ and H₂O, the organic layer separated, dried over MgSO₄ and evaporated under reduced pressure. The residue was partitioned between EtOAc and 2N HCI and the aqueous layer was then basified with 2N aqueous NaOH and extracted with EtOAc (3x). The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure to afford a residue which was purified on silica gel, eluting with CH₂C1₂ to afford the subtitle compound as a colourless solid (1.69g, 53%). RfO.55 (EtOAc/hexane 1/1, v/v). MS m/z 322 (MNH₄ ⁺).

(f) 3.4-Dimethoxv-2-iodo-6- (1 -i4-(4-morpholinecarbonvl)-1.4-diazepan-1 vll ethylideneamino) benzonitrile

Phosphorus oxychloride (0.6ml, 6.08mmol) was added to a solution of Intermediate 4 15 (2.82g, ll.Ommol) in CH₂C1₂ (20ml) and the reaction was stirred for 20min at room temperature. This was followed by the addition of the product of step (e) (1.68g,

5.52mmol) and the reaction was heated to reflux for 18h after which time it was cooled, poured onto ice and the mixture basified with aqueous sodium bicarbonate and the product extracted with EtOAc (3x). The combined organic extracts were dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH (97/3, v/v) to give the subtitle compound as a colourless solid (2.60g, 87%). Rf 0.15 (CH₂C1₂). MS m/z 542 (MH⁺). Found: C,46.00; H,5.17; N, 12.44; C_2IH₂₈N₅O₄I O.1.CH₂C1₂ requires C,46.08; H,5.17; N,12.74%.

(g) 4-Amino-6.7-dimethoxv-5-iodo-2-F4-(4-morpholinecarbonvl)-l .4-diazepan-1yllquinoline

A solution of the product of step (f) (2.0g, 3.7mmol) in a mixture of THF (50ml) and DMPU (10ml) was cooled to -78°C and treated with a solution of lithium diisopropylamide in cyclohexane (1.5M, 2.7ml) under N₂. The reaction was warmed to 0°C and stirred for

30min after which time the reaction was again cooled to -78°C and a further portion of lithium diisopropylamide in THF (1.5M, 2.7ml) was added. The reaction mixture was warmed to room temperature and stirred for 30min, after which time it was again cooled to

AP/P/ 9 8/01 175

ΑΡ ο Ο Ο 8 1 9

-78°C and treated with a third portion of lithium diisopropyiamide in THF (1.5M, 2.0ml). The reaction was again warmed to room temperature and stirred for 20min, after which time it was quenched with H₂O and extracted with EtOAc (3x). The organic layer was washed sequentially with H₂O and saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH (98/2, v/v). The subtitle compound (1.30g, 65%) was obtained as a light brown solid. Rf 0.50 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 542 (MH⁺). Found: C,45.71; H,5.26; N,12.44; C₂₁H₂₈N₅O₄I 0.25.CH₂Cl₂ requires C,45.37; H,5.07; N,12.46%.

(h) 4-Amino-6.7-dimethoxv-2-i4-(4-morpholinecarbonvl)-1.4-diazepan-l-vn-5(thiophen-3-yl)quinoIine

To a solution of the product of step (g) (500mg, 0.92mmol) in a mixture of toluene (6ml) and EtOH (3ml) was added thiophene-3-boronic acid (236mg, 1.85mmol), tetrakis(triphenylphosphine)palladium (32mg, 0.03mmol) and IM aqueous sodium carbonate solution (lml) and the reaction mixture was heated to reflux under N₂ for 18h. On cooling, the reaction mixture was diluted with H₂O, extracted with EtOAc (3x), the combined organic layers dried over MgSO₄ and evaporated under reduced pressure. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the title compound as a colourless foam (230mg, 47%). Rf 0.50 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 498 (MH⁺). Ή NMR (CDC1₃) δ 2.05 (2H, m), 3.13 (4H, m),.3.35 (2H, m), 3.50 (3H, s), 3.63 (6H, m), 3.71 (2H, m), 3.97 (5H, m), 4.30 (2H, bs), 5.76 (IH, s), 7.10 (2H, m), 7.45 (2H, m). Found: C,57.90; H,6.19; N,13.04. C₂₅H₃₁N₅O₄S O.3.CH₂C1₂ requires C,57.85; H,6.07; N,13.32%.

Example 2

4-Amino-6.7-dimethoxv-2-F4-f4-morpholinecarbonvl)-l .4-diazepan-l-yl~l-5-(thiophen-2vPquinoline

The title compound was prepared by the method of Example 1 (h) from the compound of

Example 1(g) and thiophene-2-boronic acid. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the title compound (26%) as a colourless foam. MS m/z 498 (MH⁺). 'H NMR (CDC1₃) δ 2.05 (2H, m), 3.13 (4H, m),

API?! 9 8/01 175

APO 0 0 8 1 9

3.32 (2H, m), 3.61 (9H, m), 3.74 (2H, m), 3.97 (2H, m), 4.00 (3H, s), 4.60 (2H, bs), 5.77 (IH, s), 7.0-7.3 (IH, bs), 7.06 (IH, d), 7.15 (IH, dd), 7.52 (IH, d). Found: C,55.25; H.5.92; N,12.63. C₂₅H₃₁N₅O₄S O.7.CH₂C1₂ requires C,55.40; H,5.86; N,12.57%.

Example 3

4-Amino-6.7-dimethoxv-5-(2-furyl)-2-[4-(4-morpholinecarbonvl)-l .4-diazepan-1 yllquinoline

Example 1(g) and furan-2-boronic acid [Florentin et al., J. Heterocyclic Chem., 13, 1265 (1976)]. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃(90/10/1, v/v) to afford the title compound (62%) as a colourless foam. Rf 0.52 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1. v/v). MS m/z 482 (MH⁺). Ή NMR (CDC1₃) δ 2.06 (2H, m), 3.16 (4H, m), 3.37 (2H, m), 3.50 (2H, m), 3.60 (7H, m), 3.71 (2H, m), 3.97 (2H,

m), 4.00 (5H, m), 5.80 (IH, s), 6.50 (IH, bs), 6.60 (IH, bs), 7.0-7.3 (IH, bs), 7.62 (IH, bs).

Found; C.60.36; H,6.52; N.13.46. 'C₂₅H₃₁N₅O₅ O.25.CH₂C1₂ requires C,60.29; H,6.31; N. 13.92%.

Example 4

4-Amino-6.7-dimethoxv-5-(3-fiirvl)-2-[4-(4-morpholinecarbonvl)-l.4-diazepan-1vllquinoline

AP/P/ 9 8,01 175

The title compound was prepared by the method of Example 1(h) from the compound of Example 1(g) and furan-3-boronic acid [Florentin et al., J. Heterocyclic Chem., 13, 1265 (1976)]. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the title compound (60%) as a colourless foam. MS m/z 482 ' (MH⁺). *H NMR (CDC1₃) δ 2.05 (2H, m), 3.13 (4H, m), 3.32 (2H, m), 3.55 (3H, s), 3.65 (6H, m), 3.74 (2H, m), 3.99 (5H, m), 4.55 (2H, bs), 5.77 (IH, s), 6.50 (IH, s), 7.1-7.4 (IH, bs), 7.50 (IH, s), 7.60 (IH, s). Found: C,60.22; H,6.38; N,13.76. C₂₅H₃₁N₅O₅ O.25.CH₂C1₂ requires C.60.29; H,6.31;N, 13.92%.

Example 5

AP Ο Ο Ο 8 1 9

4-Amino-6.7-dimethoxy-2-r4-(4-morpholinecarbonvl)-l .4-diazepan-1-y 11-5-(2pvridyDquinoiine

To a solution of the compound of Example 1(g) (700mg, 1.29mmol) in dioxane (15ml) was 5 added 2-(tri-n-butylstannyl)pyridine (1.42g, 3.88mmol), tetrakis(triphenylphosphine)palladium (150mg, 0.13mmol), copper(I) iodide (37mg, 0.19mmol) and lithium chloride (271 mg, 6.5mmol) and the mixture was heated to reflux under N₂ for 18h. On cooling, the reaction mixture was concentrated under reduced pressure and the residue partitioned between 2N HCl and EtOAc. The aqueous layer was washed with three further portions of

EtOAc and then basified with 2N aqueous NaOH. The product was then extracted with dT EtOAc (3x), dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the title compound as a colourless solid (210mg, 33%). Rf 0.23 (CH₂Cl₂/MeOH/0.88NH₃90/10/1, v/v). MS m/z 493 (ΜΗ’). Ή NMR (CDC1₃) δ 2.05 (2H, m), 3.15 (4H, m), 3.32 (2H, m), 3.50 (2H, m), 3.55 (3H, s), 3.60 (2H, m), 3.68 (4H, m), 3.72 (2H, m), 3.94 (2H,

m), 4.00 (3H, s), 5.80 (IH, s), 7.16 (IH, bs), 7.38 (IH, m), 7.48 (IH, m), 7.60 (IH, s), 8.74 (IH, bs). Found: C,60.89; H.6.41; N,16.03. C₂₆H₃₂N₆O₄ O.3.CH₂C1₂ requires C,60.71; H.6.32; N,16.14%.

Example 6

4-Amino-6.7-dimethoxv-2-i4-(4-morphoIinecarbonvl)-1.4-diazepan-l-vll-5-fthiophen-3yQquinazoline (a) 3.4-Dimethoxy-2-iodobenzoic acid

A solution of the compound of Example 1(b) (115g, 0.32mol) in a mixture of 3N HCl (530ml) and EtOH (200ml) was heated to reflux for 36h. On cooling, the product was filtered, air dried and then washed with hexane. The solid was then dissolved in CH₂C1₂, dried over MgSO₄ and evaporated under reduced pressure to afford the subtitle compound as a white solid. R_f 0.38 (EtOAc). MS m/z 309 (MH⁺).

(b) 3.4-Dimethoxy-2-iodobenzoic acid, ethyl ester

AP/P/ 9 8/01175

AP Ο Ο Ο 8 1 9

To a suspension of the product of step (a) (69.3g, 0.23mol) in CH₂C1₂ at 0°C was added oxalyl chloride (25ml, 0.27mol) and DMF (0.9ml, 11.3mmol) and the reaction was stirred for 18h at room temperature. EtOH (20ml, 0.34mol) was then added to the reaction, which was stirred for a further 30min, after which time it was treated with triethylamine (78ml.

0.56mol). The reaction mixture was partitioned between CH₂C1₂ and H₂O, the organic layer separated, washed sequentially with 2N HCI (3x) and saturated brine, dried over MgSO₄and evaporated under reduced pressure to give a brown oil. The product was purified on silica gel, eluting with CH₂C1₂ to afford the subtitle compound as a brown oil (30g, 39%). Rr 0.73 (EtOAc). MS m/z 337 (MH⁺).

(c) 3.4-Dimethoxy-2-iodo-6-nitrobenzoic acid, ethvl ester

Nitronium tetrafluoroborate (1 lg, 84mmol) was added to a solution of the product of step (b) (30g, 64mmol) in acetonitrile (300ml) at 0°C and the reaction was stirred for 1.5h under N₂. The reaction mixture was then diluted with ether, basified with 2N aqueous NaOH and the aqueous layer extracted with ether (3x), the combined organic layers washed with saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The product was purified on silica gel, eluting with hexane/EtOAc (85/15, v/v) to give the subtitle compound as a yellow solid (21.3g, 87%). Rf 0.77 (EtOAc). MS m/z 382 (MH).

(d) 6-Amino-3.4-dimethoxv-2-iodobenzoic acid, ethvl ester

The subtitle compound was prepared by the method of Example 1 (e) from the product of step (c). The subtitle compound (84%) was obtained as a colourless solid. Rf 0.67 (EtOAc). MS m/z 352 (MH⁺).

AP/P/ 9 8/01 175 (e) 2.4-Dihvdroxv-6.7-dimethoxv-5-iodoquinazoline

Sodium cyanate (9g, 0.14mol) and trifluoroacetic acid (11ml, 0.14mol) were added to a stirred solution of the product of step (d) (12g, 34.2mmol) in CH₂C1₂ and the stirring continued for 18h. The reaction mixture was then evaporated under reduced pressure, H₂O was added and the resulting solid filtered, washing with water. A suspension of the solid in

H₂O (50ml) was treated with NaOH pellets (lOg) and the mixture heated to 60°C for

30min. after which time the reaction was cooled, neutralised with concentrated HCI anti the

REQWflQf PATENTS resulting solid isolated by filtration, washing with H₂O and etftHD compound

-8 JAN1S9P

I fcO.BOXCYFi

I CAUSEWAY, .⁷9^5: '

ΑΡθ 0 0 8 1 9 was obtained as a colourless solid (8.4g, 71%). Rf 0.30 (EtOAc). Ή NMR (D₆-DMSO) δ: 3.70 (3H, s), 3.94 (3H, s), 9.13 (2H, bs), 12.10 (IH, bs).

(f) 2.4-Dihvdroxv-6.7-dimethoxv-5-fthiophen-3-yl)quinazoline

The subtitle compound was prepared by the method of Example 1 (h) from the product of step (e). The subtitle compound (84%) was obtained as a pale yellow solid. Rf 0.28 (EtOAc). MS m/z 305 (MH⁺).

(g) 4-Amino-2-chloro-6,7-dimethoxv-5-(thiophen-3-vl)quinazoiine

The product of step (f) was added to a mixture of phosphorous oxychloride (9ml, 96mmol) and A/JV-dimethylaniline (1ml, 8mmol) and the reaction mixture was heated to 110°C for 5h. On cooling, the reaction mixture was poured onto ice and partitioned between 2N HCl and ether. The organic layer was separated, washed with saturated brine and evaporated to give a brown oil. This was taken up into a mixture of CH₂C1₂ (100ml) and MeOH (100ml), cooled to 0°C and saturated with NH₃. The reaction was stirred for 20h, saturated once more with NH₃ and stirred for a further 5h. The reaction mixture was evaporated to dryness under reduced pressure and the residue partitioned between EtOAc and 2N HCl. The organic layer was washed with saturated brine, dried over MgSO₄ and evaporated under reduced pressure. Trituration with methanol and filtration afforded the subtitle compound as a colourless solid (255mg, 25%). Rf 0.78 (EtOAc). MS m/z 322 (MH⁺).

(h) 4-Amino-6,7-dimethoxv-2-r4-(4-morpholinecarbonvl)-l.4-diazepan-Ι-νΠ-5(thiophen-3-vl)quinazoline

A mixture of the product of step (g) (220mg, 0.68mmol), triethylamine (0.24ml, 1.7mmol) and Intermediate 3 (250mg, l.Ommol) in n-butanol (50ml) was heated to 100°C under N₂for 5 days. After cooling, the reaction mixture was partitioned between 2N aqueous NaOH and EtOAc, the organic layer separated and washed with further portions of 2N aqueous NaOH (2x), followed by saturated brine (2x). After drying over MgSO₄ and evaporating under reduced pressure, the product was triturated with EtOAc, filtered and recrystallised from toluene to give the title compound as a colourless solid (33mg, 10%). R_f 0.08 (EtOAc). MS m/z 499 (MHQ. Ή NMR (CDC1₃) δ 2.03 (2H, m), 3.18 (4H, m), 3.35 (2H,

m), 3.50 (3H, s), 3.55 (2H, m), 3.65 (4H, m), 3.84 (2H, m), 3.99 (5H, m), 4.71 (2H, bs),

AP/P/ 9 8/01 175

APO Ο 0 8 19

6.90 (IH, s), 7.12 (IH, d), 7.30 (1H, d), 7.50 (IH, dd). Found: C,57.86; H,6.03; N,16.45. C₂₄H₃₀N₆O₄S requires C,57.82; H,6.07; N,16.85%.

Example 7

4-Amino-6.7-dimethoxv-2-r4-(4-morpholinecarbonyl')-1.4-diazepan-l-vll-5-(3pvridvDquinazoline (a) 4-Amino-6.7-dimethoxv-2-hvdroxv-5-iodoquinazoline. sodium salt A suspension of the compound of Example 1(e) (9.16g, 30mmol) in CH₂C1₂ (200ml) was treated with sodium cyanate (7.9g, 120mmol) and trifluoroacetic acid (8.4ml, 105mmol) dropwise at room temperature under N₂ and the reaction stirred for 60h. The mixture was then evaporated under reduced pressure and the resulting solid suspended in a mixture of aqueous NaOH (20g in 150ml) and MeOH (200ml) and the reaction stirred at room temperature for lh. The resulting orange solution was then evaporated under reduced pressure to remove MeOH and the aqueous suspension formed was treated with EtOAc, filtered and the solid washed sequentially with H₂O (3x), acetone (3x) and ether to afford the subtitle compound as a pale yellow solid (7.75g, 69%). Rf 0.53 (CH₂Cl₂/MeOH/0.88NH₃ 84/14/2, v/v). MS m/z 322 (MHQ.

(b) 4-Amino-2-chloro-6.7-dimethoxv-5-iodoquinazoline

DMF (1.8ml, 23.0mmol) was added dropwise to phosphorus oxychloride (5.4ml, 57.6mmol) and this was followed by addition of the product of step (a) (4.0g, 11.5mmol). The resulting mixture was heated to 90°C for 30min, after which time a further quantity (5ml) of phosphorus oxychloride was added and heating continued for 18h. The reaction mixture was cooled and carefully poured onto a mixture of EtOAc (400ml) and H₂O (200ml), the mixture was neutralised with aqueous sodium bicarbonate and the aqueous layer extracted with EtOAc (2x), the combined organic layers combined, washed with saturated brine, dried over MgSO₄ and evaporated under reduced pressure to give a brown solid. This was suspended in 2N aqueous NaOH (300ml), dioxane (100ml) was added and the mixture heated to 90°C with rapid stirring for 2min. On cooling, a solid separated out and this was collected by filtration, washing sequentially with H₂O and acetone and drying

AP/P/ 9 8/01175

APO 0 0 8 1 9 in vacuo at 60°C to give the subtitle compound as an off-white solid (2.79g, 66%). IQ 0.76 (EtOAc). MS m/z 366, 368 (MH⁺).

(c) 4-Amino-6.7-dimethoxv-5-iodo-2-r4-(4-morpholinecarbonyl)-1.4-diazepan-l5 vllquinazoline

The subtitle compound was prepared by the method of Example 6(h) from the product of step (b). The subtitle compound was obtained in quantitative yield as a light brown foam. Rf 0.41 (EtOAc). MS m/z 543 (MH⁺).

(d) 4-Amino-6.7-dimethoxv-2-[4-(4-morpholinecarbonyl)-1.4-diazepan-l-vl1-5-(3pvridvDquinazoline

To a solution of the product of step (c) (300mg, 0.55mmol) in a mixture of THF (25ml) and H₂O (5ml) was added 3-pyridyldiethyl borane (485mg, 3.3mmol), tetrakis(triphenylphosphine)palladium (64mg, 0.055mmol) and potassium hydroxide (600mg, 10.7mmol) and the mixture was heated to reflux for 18h under N₂. After cooling, the reaction mixture was partitioned between EtOAc and 2N aqueous NaOH, the aqueous layer separated and extracted with two further quantities of EtOAc. The combined organic layers were washed with saturated brine, dried over MgSO₄ and evaporated under reduced pressure to give a foam. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) to afford the title compound as a colourless foam (42mg, 15%). Rf 0.10 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 494 (MH⁺). Ή NMR (CDCI.) δ 2.00 (2H, m), 3.18 (4H, m), 3.35 (2H, m), 3.50 (3H, s), 3.55 (2H, m), 3.67 (4H, m), 3.84 (2H, m), 3.97 (2H, m), 4.00 (3H, s), 4.48 (2H, bs), 6.97 (1H, s), 7.45 (1H, m), 7.74 (1H, m), 8.68 (1H, m), 8.74 (1H, m). Found: C,59.85; H,6.42; N,18.54. C₂₄H₃₁N₇O₄ 0.2.EtOAc 0.5.H₂O requires

C,59.57; H,6.51; N,18.85%.

Example 8

4-Amino-6.7-dimethoxv-2-[4-(4-morpholinecarbonvl)-1.4-diazepan-l-vll-5-(2-pyridvl)quinazoline

The title compound was prepared by the method of Example 5 from the compound of

Example 7(c). The product was purified on silica gel eluting with CH₂Cl₂/MeOH (95/5,

AP/P/ 9 8/01 175

APO 0 0 8 1 9 v/v) followed by trituration with hexane/EtOAc and recrystallisation from toluene to afford the title compound (19%) as a colourless solid. Rf 0.25 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 494 (MH⁺). Ή NMR (CDClj δ: 2.00 (2H, p), 3.15 (4H, t), 3.30 (2H, dd), 3.45-3.58 (2H, m), 3.50 (3H, s), 3.65 (4H, t), 3.82 (2H, t), 3.94 (2H, t), 3.97 (3H, s), 4.55 (2H, s), 6.94 (IH, s), 7.39 (IH, m), 7.42 (IH, d), 7.82 (IH, t), 8.77 (IH, d). Found: C,59.91; H,6.27, N,19.23; C₂₅H₃₁N₇O₄ 0.5.H₂O requires C,59.75; H,6.42, N,19.50%.

Example 9

4-Amino-6.7-dimethoxv-5-(2-pvridvl)-2-(5.6.7.8-tetrahvdro-1.6-naphthvrid-610 vPquinazoline (a) 4-Amino-6.7-dimethoxv-5-iodo-2-(5,6.7.8-tetrahvdro-1.6-naphthvrid-6vpquinazoline

The subtitle compound was prepared by the method of Example 6(h) from the compound of Example 7(b) and 5,6,7,8-tetrahydro-l,6-naphthyridine [Shiozawa et al., Chem. Pharm. Bull., 32. 2522 (1984)]. The subtitle compound was obtained in quantitative yield as a brown foam. R_f 0.35 (CH₂Cl,/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 464 (MET).

(b) 4-Amino-6.7-dimethoxv-5-(2-pvridvl)-2-(5.6.7,8-tetrahvdro-1.6-naphthvrid-620 vPquinazoline

The title compound was prepared by the method of Example 5 from the product of step (a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (98/2, v/v) to afford the title compound (30%) as a pale yellow solid. Rf 0.13 (CEt₂Cl₂/MeOH 95/5, v/v). MS m/z 415 (MHJ. Ή NMR (CDC1₃) δ: 3.08 (2H, t), 3.52 (3H, s), 3.97 (3H, s), 4.20 (2H, t), 4.67 (2H, bs), 5.00 (2H, s), 7.03 (IH, s), 7.12 (IH, m), 7.40 (IH, m), 7.48 (2H, m), 7.84 (IH, dt), 8.40 (IH, d), 8.78 (IH, d). Found: C,65.17; H,5.27, N,19.64; C₂₃H₂₂N₆O₂ 0.5.H₂O requires C,65.24; H,5.48, N, 19.84%.

Example 10

4-Amino-6.7-dimethoxv-5-(2-pvrimidvP-2-(5.6.7.8-tetrahvdro-1.6-naphthvrid-6yPquinazoline

APO 0 0 8 1 9

The title compound was prepared by the method of Example 5 from the compound of Example 9(a) and 2-(tri-n-butylstannyl)pyrimidine [Sandosham et al., Tetrahedron, 50, 275 (1994)]. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) followed by partitioning between 2N HCI and EtOAc, washing the aqueous layer with EtOAc (3x), basiiying with 2N aqueous NaOH and extracting with EtOAc (3x). The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure to afford the title compound (21%) as a pale yellow solid. R^· 0.39 (CH₂Cl₂/MeOH 9/1, v/v). MS m/z 416 (MH⁺). Ή NMR (CDC1₃) δ: 3.06 (2H, t), 3.68 (3H, s), 3.98 (3H, s), 4.20 (2H, t), 4.61 (2H, bs), 5.00 (2H, s), 7.06 (1H, s), 7.13 (1H, m), 7.38 (1H, m), 7.50 (1H, d), 8.43 (1H, d), 8.92 (2H, d). Found: C,61.99; H,5.08, N,22.11; C₂₂H₂₁N₇O₂ 0.15.CH₂Cl₂0.1.EtOAc requires C,61.98; H,5.10, N,22.44%.

Example 11

4-Amino-6.7-dimethoxv-5-(2-pvrimidvl)-2-f5.6.7,8-tetrahvdro-1.3.6-triazanaphth-6yQquinazoline (a) l-(t-Butvloxvcarbonvl)-3-(N./V-dimethvlaminomethylidene')-4-piperidone

DMF dimethyl acetal (5.82ml, 0.044mol) was added to a stirred solution of l-boc-4piperidone [Ashwood et al., J. Chem. Soc., Perkin 1, 641 (1995)] (8.73g, 0.044mol) in DMF (80ml) and the reaction mixture was heated to 80°C under N₂ for 18h. After cooling, the DMF was removed under reduced pressure and the residue was partitioned between EtOAc and H₂O, the organic layer washed with H₂O and saturated brine, then dried over MgSO₄ and evaporated to afford the subtitle compound as a solid (8.44g, 76%). Rj· 0.33 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 255 (MH⁺).

(b) 6-(t-Butyloxvcarbonvl)-(5,6.7.8-tetrahydro-1.3,6-tri azanaphthalene)

Sodium (762mg, 0.033mol) was added to EtOH (150ml) followed by formamidine acetate (3.45g, 0.033mol) and the reaction was stirred at room temperature under N₂ for 30min. A solution of the product of step (a) (8.43g, 0.033mol) in EtOH (50ml) was then added and the reaction heated to reflux for 18h after which time the mixture was cooled and ur>

r*» ' s» OO ⁽'Λ £

ci

I·'· ^{1 1 1} »*- ' concentrated under reduced pressure. The residue was partifione^^beP^^i^E^i^.^and H₂O, the organic layer washed with saturated brine and dried |ver on ?

I »8 JAN19P·’

PO. BOX CY 1'7 CAUSEWAY, _;

APO 0 0 8 1 9 silica gel, eluting with CH₂Cl₂/MeOH (96/4, v/v) afforded the subtitle compound as an oil (5.09g, 65%). 0.57 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 236 (MH⁺).

(c) 5.6.7.8-Tetrahvdro-l .3.6-triazanaphthalene hydrochloride

HCI was bubbled through a solution of the product of step (b) (4.80g, 0.020mol) in a mixture of MeOH and ether (50ml, 1/1, v/v) at 0°C until saturated. The mixture was then allowed to reach room temperature over 2h, after which time a precipitate formed. This was isolated by decanting off the supernatant solution, washing with ether (2x) and drying in vacuo to afford the subtitle compound as a colourless solid (2.85g, 81%). Rf 0.13 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 136 (MH⁺).

(d) 4-Amino-6.7-dimethoxv-5-iodo-2-(5.6-7.8-tetrahvdro-1.3.6-triazanaphth-6vDquinazoline

The subtitle compound was prepared by the method of Example 6(h) from the product of 15 step (b) and the compound of Example 7(b). The subtitle compound (65%) was obtained as a colourless solid. Rf 0.52 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 465 (MH’).

(e) 4-Amino-6.7-dimethoxv-5-(2-pvrimidvl)-2-(5.6.7.8-tetrahydro-1.3.6-triazanaphth6-vl)quinazoIine

The title compound was prepared by the method of Example 5 from the product of step (d) and 2-(tri-n-butylstannyl)pyrimidine. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) to afford the title compound (15%) as a colourless foam. Rf 0.30 (CH₂Cl₂/MeOH 9/1, v/v). MS m/z 417 (MH⁺). Ή NMR (CDC1₃) δ: 3.03 (2H, t), 3.68 (3H, s), 4.00 (2H, m), 4.22 (2H, t), 4.47 (2H, bs), 5.00 (2H, s), 6.94 (1H, s), 7.07 (1H, s), 7.38 (1H, t), 8.55 (IH, s), 8.95 (2H, d), 9.00 (1H, s). Found: C,56.61; H,4.73, N,24.84;

C_2IH₂₀N₈O, O.5.CH₂C1₂ requires C,56.26; H,4.61, N,24.42%.

Example 12

4-Amino-2-(7-aminosulfonvl-1.2.3.4-tetrahydroisoquinol-2-vl)-6.7-dimethoxv-5-(230 pvridvDquinazoline

AF/F/ 9 8/61 175 (a) 4-Amino-2-chloro-6.7-dimethoxv-5-(2-pvridyl)quinazoline

ΑΡ Ο Ο Ο 8 1 9 w

To a solution of the compound of Example 7(b) (l.Og, 2.7mmol) in dioxane (20ml) was added 2-(tri-n-butylstannyl)pyridine (l.lg, 3.0mmol), lithium chloride (1.5g, 35mmol), tetrakis(triphenylphosphine)palladium (320mg, 0.27mmol) and copper(I) iodide (78mg, 0.41 mmol) and the reaction was heated to 100°C for 2h. After cooling, the reaction mixture was partitioned between 2N HCI and EtOAc, the aqueous layer was extracted further with EtOAc (3x) then basified with 2N aqueous NaOH and extracted with EtOAc (3x). The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure to give a pale yellow solid. This was suspended in ether and filtered to afford the subtitle compound as a colourless solid, (660mg, 76%). Rf 0.53 (EtOAc). MS m/z 317, 319 (MH⁺).

(b) 4-Amino-2-(7-aminosulfonvl-1.2.3.4-tetrahydroisoquinol-2-vl)-6.7-dimethoxy-5(2-pyridvl)quinazoline

To a solution of the product of step (a) (250mg, 0.8mmol) in n-butanol/DMA (3:1, v/v, 8ml) was added l,2,3,4-tetrahydroisoquinoline-7-sulfonamide hydrochloride (300mg,

1.2mmol) and triethylamine (0.33ml, 2.4mmol) and the reaction mixture heated to 100°C under N, for 18h. The reaction was then cooled, partitioned between EtOAc and 2N aqueous sodium hydroxide, the aqueous layer separated and extracted with EtOAc, the combined organic layer washed with H₂O, dried over MgSO₄ and evaporated under reduced pressure. Purification on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (95/5/0.5, v/v) afforded an oil which was dissolved in CH₂Cl₂/MeOH and the product precipitated out with hexane to afford, on filtration and drying in vacuo, the title compound as a colourless solid (198mg, 50%). Rf 0.50 (CH₂Cl₂/MeOH/0.88NH₃ 84/14/2, v/v). MS m/z 493 (MH⁺). Ή NMR (D₆-DMSO) δ: 0.90 (2H, m), 3.42 (3H, s), 3.94 (3H, s), 4.00 (2H, m), 4.94 (2H, s), 5.50 (2H, bs), 6.94 (IH, s), 7.26 (2H, s), 7.32 (IH, d), 7.40-7.55 (2H, m), 7.55-7.68 (2H,

m), 7.94 (IH, t), 8.71 (IH, d). Found: C,58.57; H,5.35, N,15.75; C₂₄H₂₄N₆O₄S 0.4.hexane

0.9.H₂O requires C,58.97; H,5.73, N,15.64%.

AF/P; 3 8/01175

Example 13

4-Amino-6.7-dimethoxv-2-_!(2-isoindoIinvl)-5-(2-pvridvl)quinazoline

The title compound was prepared by the method of ExampleJWfttT^n¹ ΐίϊε compouncTof

Example 12(a) and isoindoline hydrochloride. The product waF^^^^^J^iga gelj

I -8 JAN19?

| PO.BOXCV' . · | CAUSEWAY?.?.·.'

APO Ο Ο 8 1 9 eluting with EtOAc followed by trituration with CH₂C1₂ and ether to afford the title compound (51%) as a colourless foam. Rf 0.42 (EtOAc). MS m/z 400 (ΜΗ*). ’H NMR (CDClj δ: 3.55 (3H, s), 4.03 (3H, s), 4.70 (2H, s), 4.97 (4H, s), 7.08 (1H, s), 7.21-7.50 (6H, m), 7.84 (1H, t), 8.88 (1H, d). Found: C,65.15; K5.21, N,15.54; C₂₃H₂₁N₅O₂

O.4.CH₂C1₂ 0.25.ether requires C,64.83; H,5.42, N,15.50%.

Example 14

4-Amino-6.7-dimethoxv-5-(2-pvridvl)-2-(5.6.7.8-tetrahvdro-1.3.6-tria7anaphth-6vDquinazoline

The title compound was prepared by the method of Example 12(b) from the compound of Example 12(a) and the compound of Example 11(c). The product was purified on silica gel, eluting with EtOAc/MeOH (95/5, v/v) followed by trituration with ether to afford the title compound (35%) as a colourless solid. Rf 0.18 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v).

MS m/z 416 (MHJ. Ή NMR (CDClj δ: 3.03 (2H, t), 3.52 (3H, s), 4.00 (3H, s), 4.20 (2H, t), 4.74 (2H, s), 5.00 (2H, s), 7.03 (1H, s), 7.40 (1H, m), 7.45 (1H, d), 7.84 (1H, t), 8.55 (1H, s), 8.78 (1H, d), 9.00 (1H, s). Found: C,60.94; H,5.13; N,21.93; C₂₂H₂₁N₇O₂O.3.CH₂C1₂ requires C,60.74; H,4.94; N,22.24%.

Example 15

4-Amino-6.7-dimethoxv-2-(7-methanesulfonamido-2.3.4.5-tetrahvdro-lH.3-benzazepin-3vl)-5-(2-pvridvl)quinazoline (a) 3-t-Butvloxvcarbonvl-7-nitro-2.3.4.5-tetrahydro-lH.3-benzazepine

To a solution of 7-nitro-2,3,4,5-tetrahydro-lH,3-benzazepine [Pecherer et al., J. Heterocyclic Chem. 8, 779 (1971)] (1.92g, O.Olmol) in CH₂C1₂ (40ml) at 0°C was added dropwise a solution of di-(t-butyl) dicarbonate in CH₂C1₂ and the reaction allowed to stir at room temperature for 18h. The reaction mixture was evaporated under reduced pressure to afford an oil which was taken up into CH₂C1₂, washed with aqueous sodium carbonate solution (3x), IN HCI (3x) and saturated brine (2x). The organic layer was separated, dried over MgSO₄ and evaporated to afford an oil. Trituration with hexane afforded the subtitle

AP/P/ 9 8/01 1 7 5

APO 0 0 8 1 9 compound as a colourless solid (2.33g, 80%). Rf 0.8 (CH₂Cl₂/MeOH 95/5, v/v). M.p. 106108°C.

(b) 7-Amino-3-t-butyloxvcarbonyl-2.3.4.5-tetrahvdro-lH.3-benzazepine

A solution of the product of step (a) (2.lg, 7.2mmol) in a mixture of EtOAc (20ml) and MeOH (20ml) was hydrogenated over palladium on charcoal (5% w/w, lOOmg) at 345kPa (50p.s.i.) and room temperature for 3h. After filtering, the filtrate was evaporated under reduced pressure to give the subtitle compound as an oil (2.0g, quantitative). Rf 0.7 (CH₂Cl₂/MeOH 9/1, v/v). Found: C,68.96; H,8.63; N,10.33; C₁₅H₂₂N₂O₂ requires C,68.67;

H,8.45; N, 10.68%.

-J (c) 3-t-Butyloxycarbonvl-7-methanesulfonamido-2.3.4.5-tetrahydro-lH.3-benzazepine

Methanesulfonyl chloride (0.56ml, 7.3mmol) was added dropwise to a solution of the product of step (b) (1.9g, 7.2 mmol) in pyridine (40ml) at 0°C and the resulting orange solution was allowed to stir for 18h. The reaction was evaporated under reduced pressure to give an oil which was dissolved in CH₂C1₂ and extracted sequentially with aqueous sodium bicarbonate solution (3x) and saturated brine (3x). The organic layer was separated, dried over MgSO₄ and evaporated under reduced pressure to give an oil. The product was . purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) followed by trituration with ether to give the subtitle compound as a white solid (1.2g, 49%). Rf 0.5 (CH₂Cl₂/MeOH 95/5, v/v). M.p. 153-154°C.

(d) 7-Methanesulfonamido-2,3.4.5-tetrahydro-lH.3-benzazepine hvdrochloride

The subtitle compound was prepared from the product of step (d) by the method of

Example 11(c). The subtitle compound (71%) was obtained as a colourless solid. Rf 0.25 (CH₂Cl₂/MeOH/0.88NH₃ 84/14/2, v/v).

(e) 4-Amino-6.7-dimethoxv-2-(7-methanesulfonamido-2.3.4.5-tetrahydro-lH-3benzazepin-3-vl)-5-(2-pvridvl)quinazoline

The title compound was prepared by the method of Example 12(b) from the product of step (d) and the compound of Example 12(a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (97/3/0.5, v/v) to give the title compound (40%) as a

AP/P/ 9 8/01 175

AP Ο Ο Ο 8 1 9 π η 33 <,«· colourless solid. Rf 0.31 (EtOAc/MeOH 95/5, v/v). MS m/z 521 (MH*). ^lH NMR (CDC1₃) δ: 2.90 (4H, bm), 3.00 (3H, s), 3.53 (3H, s), 4.00 (7H, bm), 4.65 (2H, bs), 6.68 (IH, bs), 6.96 (2H, s), 7.03 (IH, s), 7.10 (IH, m), 7.42 (IH, m), 7.48 (IH, m), 7.95 (IH, t), 8.80 (IH, d). Found: C,56.65; H,5.26; N,14.66; C₂₆H₂₈N₆O₄S O.5.CH₂C1₂ requires C,56.53; H,5.19;

N,14.93%.

Example 16

4-Amino-6.7-dimethoxv-2-[7-(4-morphoIinesulfonamido)-1.2.3.4-tetrahydroisoquinolin-2vl1-5-(2-pyridvl)quinazoline

10.

(a) 7-(4-Morpholinesulfonamido)-1.2.3.4-tetrahydroisoquinoline

To a solution of 2-trifluoroacetyl-1,2,3,4-tetrahydroisoquinoline-7-sulfonyl chloride [Blank et al J. Med. Chem. 23, 837 (1980)] (l.Og, 3.3mmol) in THF (40ml) was added morpholine (0.74ml, 8.5mmol) and a thick white precipitate was formed immediately. After 5min a solution of sodium carbonate (1.7g, 16.5mmol) in H₂O (20ml) was added followed by a mixture of MeOH and H_;O (20ml, 1/1, v/v). The resulting clear solution was stirred for 18h at room temperature after which time the reaction was partitioned between EtOAc and 2N aqueous NaOH and the aqueous layer was separated and extracted with EtOAc (8x). The combined organic layers were washed with brine, dried over MgSO₄ and evaporated to give the subtitle compound as a colourless solid (650mg, 70%). Rf 0.50 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 283 (MH⁺).

(b) 4-Amino-6.7-dimethoxv-2-[7-(4-morpholinesulfonamido)-1.2.3.4-tetrahydroisoquinolin-2-vl]-5-(2-pvridvl)quinazoline

The title compound (40%) was prepared by the method of Example 12(b) from the product of step (a) and the compound of Example 12(a). MS m/z 563 (MH⁺). Ή NMR (CDC1₃) δ:

' 3.00 (6H, m), 3.53 (3H, s), 3.73 (4H, m), 3.98 (3H, s), 4.12 (2H, t), 4.70 (2H, bs), 5.07 (2H,

s), 7.05 (IH, s), 7.32 (IH, d), 7.40 (IH, m), 7.48 (IH, d), 7.53 (IH, d), 7.60 (IH, s), 7.84 (IH, t), 8.78 (IH, d).

Example 17

AP/P/ 9 8/01 1/5

AP Ο Ο Ο 8 1 9

4-Amino-6.7-dimethoxv-2-(2-methvl-5.6.7.8-tetrahydro-1.3.6-triazanaphth-6-yl)-5-(2pyridvDquinazoline (a) 6-ft-ButyloxvcarbonvO-2-methyl-(5.6.7.8-tetrahydro-1.3.6-triazanaphthalene

A solution of sodium ethoxide in EtOH was prepared by the addition of sodium (690mg, 30.0mmol) to EtOH (75ml) and was treated with the compound of Example 11(a) (7.62g, 30.0mmol) and acetamide hydrochloride (3.12g, 33.0mmol). The reaction was heated to reflux for 18h, after which time it was partitioned between EtOAc and aqueous sodium bicarbonate, the organic layer was separated and dried over MgSO₄ and evaporated under reduced pressure to afford an oil. The product was purified on silica gel, eluting with EtOAc/MeOH (98/2, v/v) to afford the subtitle compound as a crystalline solid (6.47g, 87%). Rf 0.31 (EtOAc/MeOH 95/5, v/v). Ή NMR (CDC1₃) δ: 1.48 (9H, s), 2.68 (3H, s), 2.92 (2H, m), 3.73 (2H, m), 4.57 (2H, s), 8.38 (IH, s) (b) 2-Methvl-5.6.7.8-tetrahvdro-1.3.6-triazanaphthalene hydrochloride

The subtitle compound was prepared by the method of Example 11 (c) from the product of step (a). The product was partitioned between EtOAc and 2N aqueous NaOH and the aqueous layer extracted repeatedly with EtOAc. The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure to afford the subtitle compound (7%) as a yellow oil that crystallised on standing. ’H NMR (CDC1₃) δ: 2.70 (3H, s), 2.90 (2H, t), 3.25 (2H, t), 4.00 (2H, s), 8.38 (IH, s).

(c) 4-Amino-6.7-dimethoxv-2-(2-methyl-5.6.7.8-tetrahydro-1.3.6-triazanaphth-6-vl)-5(2-pyridyl)quinazoline

The title compound was prepared by the method of Example 12(b) from the product of step (b) and the compound of Example 12(a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (94/6, v/v) to afford the title compound (32%) as a colourless foam. Rf 0.33 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 430 (MH⁺). Ή NMR (CDC1₃) δ: 2.65 (3H, s), 2.97 (2H, m), 3.50 (3H, s), 3.98 (3H, s), 4.15 (2H, t), 4.73 (2H, bs), 4.93 (2H, bs),

7.00 (IH, s), 7.40 (2H, m), 7.82 (IH, t), 8.42 (IH, s), 8.75 (IH, d). Found: C,56.65; H,5.26;

N,14.66; C₂₃H₂₃N₇O₂ 0.3.CH₂Cl₂ requires C,56.53; H,5.19; N,14.93%.

APiPt 9 8/01 175

ΑΡ Ο Ο Ο 8 1 9

Example 18

4-Amino-6.7-dimethoxv-5-(2-pvridvI)-2-(5-6.7.8-tetrahvdro-1.3.7-triazanaphth-7-yl)quinazoline (a) 1 -Tritvl-3-piperidone

Trityl chloride (13.1g, 47.0mmol) was added to a stirred suspension of 3-piperidone hydrochloride (5.79g, 42.7mmol) and triethylamine (14.9ml, 107mmol) in CH,C1₂ (100ml) and the reaction was stirred for 16h under N₂ at room temperature. The resulting mixture was filtered and the filtrate washed sequentially with H₂O and 5% aqueous citric acid, dried over MgSO₄ and evaporated under reduced pressure. Trituration with pentane afforded the subtitle compound as a colourless solid (4.8g, 33%). Rf 0.23 (CH₂Cl₂/pentane 2/3, v/v). Ή NMR (CDC1₃) δ: 2.05 (2H, m), 2.35 (2H, m), 2.45 (2H, m), 2.85 (2H, s), 7.067.55 (15H, m).

(b) 4-(MJV-Dimethvlaminomethvlidene)-l-tritvl-3-piperidone

The subtitle compound was prepared by the method of Example 11(a) from the product of step (a). Crystallisation from ether afforded the subtitle compound (52%) as a colourless solid. Rf 0.23 (CH₂Cl₂/pentane 2/3, v/v). Ή NMR (CDC1₃) δ: 2.35 (2H, t), 2.87 (2H, t), 2.97 (2H, s), 3.13 (6H, s), 7.13 (3H, m), 7.24 (7H, m), 7.50 (6H, m).

(c) 7-Trityl-5.6.7.8-tetrahvdro-1.3.7-tri azanaphthalene

The subtitle compound was prepared by the method of Example 11 (b) from the product of step (b). The product was purified by chromatography on silica gel, eluting with CH₂Cl₂/ether (9/1, v/v) to afford the subtitle compound (51%). Rf 0.33 (CH₂Cl₂/ether

85/15, v/v). Ή NMR (CDC1₃) δ: 2.60 (2H, t), 2.97 (2H, t), 3.58 (2H, s), 7.06-7.37 (8H, m),

7.52 (7H, m), 8.45 (IH, s), 8.90 (IH, s).

s L t 10/86 /cJ/dV (d) 5.6.7.8-Tetrahvdro-1.3.7-triazanaphthalene hydrochloride

The subtitle compound was prepared by the method of Example 11(c) from the product of step (c). The product crystallised from MeOH/ether to afford the subtitle compound (65%) as an orange hygroscopic solid. Ή NMR (d₆-DMSO) δ: 3.06 (2H, m), 3.40 (2H, m), 4.26 (2H, s), 8.68 (IH, s). 9.00 (IH. s). 9.96 (2H, bs).

APO Ο Ο 819

(e) 4-Amino-6.7-dimethoxy-5-(2-pyridvl)-2-f5.6,7.8-tetrahydro-l ,3,7-triazanaphth-7vD-quinazoIine

The title compound was prepared by the method of Example 12(b) from the product of step 5 (d) and the compound of Example 12(a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) to give the title compound (66%) as a light brown solid. Rf

0.20 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 416 (MH⁺). *H NMR (CDC1₃) δ: 2.92 (2H, t), 3.52 (3H, s), 4.00 (3H, s), 4.18 (2H, t), 4.70 (2H, bs), 5.18 (2H, bs), 7.05 (IH, s), 7.41 (IH, m), 7.43 (IH, m), 7.83 (IH, t), 8.50 (IH, s), 8.79 (IH, d), 9.02 (IH, s). Found: C,61.24; H,4.91;

N,22.35; C₂₂H₂₁N₇O₂ O.25.CH₂C1₂ requires C,61.20; H,4.96; N,22.46%.'

Example 19

4-Amino-6.7-dimethoxy-2-(5-methanesulfonamido-1.2.3.4-tetrahvdroisoquinol-2-yl)-5-(2pvridvDquinazoline (a) 5-Methanesulfonamidoisoquinoline

Methanesulfonyl chloride (3.2ml, 42mmol) was added to a solution of 5-aminoisoquinoline (5.0g, 35mmol) in pyridine (40ml) and the mixture was allowed to stand for 72h. The reaction mixture was then poured into aqueous citric acid (10%, 400ml) and extracted with

EtOAc (2x230ml). The organic layer was evaporated to give a residue which was purified on silica gel, eluting with CH₂Cl₂/MeOH to afford the subtitle compound as a solid (3.55g, 46%). Rf 0.03 (CH₂Cl₂/ether 4/1, v/v). Ή NMR (D₅-DMSO) δ: 3.07 (3H, s), 7.68 (IH, t), 7.75 (IH, d), 8.03 (IH, d), 8.10 (IH, d), 8.54 (IH, d), 9.32 (IH, s), 9.79 (IH, bs).

m r*·»

O oo (b) 5-Methanesulfonamido-l -2.3.4-tetrahydroisoquinoline hydrochloride

A solution of the product of step (a) (3.50g, 15.7mmol) in EtOH (205ml) was treated with platinum dioxide (1.5g) and IN HCI (15.7ml). The mixture was hydrogenated at 414kPa (60p.s.i.) for 16h, after which time the reaction was filtered. The filtrate was evaporated under reduced pressure and triturated with CH₂C1₂ to afford the subtitle compound as a colourless solid. The solid residue from the filtration was taken up into MeOH/H₂O (1/2, v/v), the suspension filtered, washed with CH₂C1₂ (3x) and-jlK¹ flltl'dle evaporated to afford a second crop of the subtitle compound (total yijpld 0i21 | -8 JANW

RO.BOXCY’T

CAUSEWAY -'<>,·...

___________—··. —·.......

AP O Ο Ο β 1 9 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). ^!Η NMR (d₆-DMSO) δ: 2.96-3.10 (2H, m), 3.31 (3H, m), 4.21 (2H, s), 7.12 (IH, m), 7.26 (2H, m), 9.24 (IH, s), 9.61 (2H, bs).

(c) 4-Amino-6.7-dimethoxv-2-(5-methanesulfonamido-1.2.3.4-tetrahvdroisoquinol-25 vl)-5 -(2-pyridvl)quinazoline

The title compound was prepared by the method of Example 12(b) from the product of step (b) and the compound of Example 12(a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) to give the title compound (80%) as a light brown solid. Rf 0.21 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 507 (MH⁺). ’H NMR (CDCI₃) δ: 2.80 (2H, t), 3.02 (3H, s), 3.53 (3H, s), 4.00 (3H, s), 4.12 (2H, t), 4.67 (2H, bs), 4.97 (2H, s), 6.15 (IH, s),

7.03 (IH, s), 7.10 (IH, d), 7.21 (IH, d), 7.32 (IH, d), 7.42 (IH, m), 7.46 (1H, d), 7.84 (IH, t), 8.79 (IH, d). Found: C,55.09; H,4.90; N,14.94 C₂₅H₂₆N₆O₄S O.56.CH₂C1₂ requires C,55.38; H,4.93; N,15.16%.

Example 20

4-Amino-6.7-dimethoxv-2-r7-( 1 -piperazinesulfonvl)-1,2,3 A-tetrahydroisoquinolin^-vll-S(2-pyridvl)quinazoline (a) 7-(4-t-Butvloxvcarbonvl-l-piperazinesulfonvl)-1.2.3.4-tetrahvdroisoquinoline

The subtitle compound was prepared by the method of Example 16(a) from 1-tbutyloxycarbonylpiperazine and 2-trifluoroacetyl-1,2,3,4-tetrahydroisoquinoline-7-sulfonyl chloride. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (93/7, v/v) to afford the subtitle compound (35%) as a colourless solid. Rf 0.56 (CH₂Cl₂/MeOH 9/1, v/v). MS m/z 382 (MH⁺).

(b) 4-Amino-6.7-dimethoxv-2-i7-(4-t-butvloxvcarbonvl-l-piperazinesulfonvl)-1.2.3.4tetrahvdroisoquinolin-2-vn-5-(2-pyridvI)quinazoline

The subtitle compound was prepared by the method of Example 12(b) from the product of step (a) and the compound of Example 12(a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (96/4, v/v) to give the subtitle compound (69%) as a colourless solid. Rf 0.25 (CH₂Cl₂/MeOH 96/4, v/v).

S L I t 0 ! 8 6 /d/dV

AP Ο Ο Ο 8 1 9 (c) 4-Amino-6.7-dimethoxv-2-r7-f 1-piperazinesulfonvl)-1.2.3 N-tetrahydroisoquinolin2-vIl-5-f2-pvridyl)quinazoline trihydrochloride

The title compound was prepared by the method of Example 11(c). The product was triturated with CH₂C1₂ to give the title compound (58%) as a colourless solid. MS m/z 562 (MH⁺). Ή NMR (d₆-DMSO) δ: 3.03-3.25 (1 IH, m), 3.50 (3H, s), 4.00 (3H, s), 4.12 (2H, t),

5.17 (2H, bs), 5.58 (IH, bs), 7.52-7.68 (5H, m), 7.94 (IH, s), 8.07 (IH, t), 8.62 (IH, bs), 8.80 (IH, d), 9.20 (2H, bs), 12.72 (IH, bs). Found: C,46.88; H,5.61; N,13.68; C₂₈H₃₁N₇O₄S 3.HC1 2.5.H₂O requires C,46.96; H,5.48; N,13.69%.

Example 21

4-Amino-2-[5-GV.N-diethvlaminomethvl)-1.2.3.4-tetrahvdroisoquinolin-2-vl~|-6.7dimethoxv-5-(2-pvridyl)quinazoline (a) 5-(Trifiuoromethanesulfonato)isoquinoline

Pyridine (8.35ml, O.lOmol) was added to a solution of 5-hydroxyisoquinoline (5.0g, 0.034mol) in CH₂C1₂, the solution was cooled to -40°C and triflic anhydride (8.47ml, 0.052mol) was added dropwise. The reaction was allowed to reach room temperature and stirred for 18h, after which time H₂O was added, the organic layer was separated, washed with saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (98/2, v/v) to give the subtitle compound as a solid (6.93g, 73%). Rf 0.70 (CH₂Cl,/MeOH 9/1, v/v).

(b) S-ftVjy-Diethylcarboxamidohsoquinoline

To a solution of the product of step (a) (500mg, 1.8mmol) in DMF (4ml) was added palladium acetate (12mg, 0.054mmol), triphenylphosphine (28mg, O.llmmol) and diethylamine (3.7ml, 36mmol) and the reaction was heated at 60°C under a balloon of CO for 20h. After cooling, the reaction mixture was diluted with saturated brine and extracted with EtOAc (3x), the combined organic extracts were dried over MgSO₄ and evaporated under reduced pressure. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (97/3, v/v) to give the subtitle compound as a solid (220mg, 53%). R_f 0.45 (CH₂Cl₂/MeOH

9/1, v/v). MS m/z 229 (MET).

L I t 0 f 8 5 M/dV

APO 0 0 8 1 9 (c) 5-f/0/-Diethvlcarboxamido)-1.2.3.4-tetrahydroisoquinoline

The subtitle compound was prepared by the method of Example 19(b) from the product of step (b). The crude product was partitioned between CH₂C1₂ and aqueous sodium bicarbonate solution and the aqueous layer was extracted with further portions of CH₂C1₂.

The combined organic extracts were dried over MgSO₄ and evaporated under reduced pressure. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃(90/10/1, v/v) to give the subtitle compound (66%) as an oil. R,· 0.09 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 233 (MH⁺).

(d) 5-(A./V-Diethvlaminomethvl')-1.2,3.4-tetrahydroisoquinoline

J

A solution of borane in THF (IM, 18ml, 18.0mmol) was added dropwise to a solution of the product of step (c) (1,39g, 6.0mmol) in THF (20ml). The reaction mixture was heated to reflux for 18h under N₂ after which time the reaction mixture was cooled, added to a mixture of 2N HCl/MeOH (1/1, v/v, 100ml) and stirred for 2h. The MeOH was removed under reduced pressure, the reaction mixture was basified to pH 10 and extracted with CH₂C1₂ (3x). The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure to give the subtitle compound as an oil (840mg, 64%). MS m/z 219 (MH⁺). Ή NMR (CDC1₃) δ: 1.05 (6H, t), 2.40 (IH, bs), 2.52 (4H, q), 2.89 (2H, t), 3.20 (2H, t), 3.47 (2H, s), 4.06 (2H, s), 6.90 (IH, d), 7.09 (IH, t), 7.20 (IH, d).

> 20 (e) 4-Amino-2-F5-(A/N-diethvlaminomethyP)-1.2.3.4-tetrahvdroisoquinolin-2-vl1-6.7dimethoxv-5-(2-pvridvI)quinazoline

The title compound was prepared by the method of Example 12(b) from the product of step (d) and the compound of Example 12(a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to give the title compound (30%) as a colourless foam. MS m/z 499 (MH⁺). Ή NMR (CDC1₃) δ: 1.05 (6H, t), 2.53 (4H, q), 3.00 ’ (2H, t), 3.50 (3H, s), 3.53 (2H, s), 4.00 (3H, s), 4.08 (2H, t), 4.73 (2H, bs), 4.98 (2H, s),

7.0-7.3 (4H, m), 7.40 (2H, m), 7.82 (IH, t), 8.77 (IH, d). Found: C,68.36; H,6.71; N,15.96;

C₂₉H₃₄N₆O₂ O.2.CH₂C1₂ requires C,68.01; H,6.72; N,16.30%.

Example 22

AP/P/ 9 8/01175

APO 0 0 8 1 9

4-Amino-6.7-dimethoxv-2-f5-methanesuIfonamido-1.2.3.4-tetrahvdroisoquinol-2-yl)-5-('2pvrimidyl)quinoline (a) 2-Acetyl-(5-methanesulfonamido)-1.2.3.4-tetrahvdroisoquinoline

To a solution of the compound of Example 19(b) (2.87g, 10.9mmol) in CH₂C1₂ at 0°C was added acetic anhydride (1.2ml, 13.1 mmol) and triethylamine (3.4ml, 24.0mmol) and the reaction was stirred at room temperature for 16h after which time the reaction mixture was partitioned between EtOAc and aqueous sodium bicarbonate solution and the aqueous phase extracted with further portions of EtOAc. The combined organic extracts were dried over MgSO₄ and evaporated to afford an oil. This was dissolved in MeOH (15ml) and treated with aqueous sodium carbonate solution (7%, w/w, 15ml) and the mixture stirred for 16h at room temperature, after which time the MeOH was removed under reduced pressure, the pH was adjusted to pH 8 with 2N aqueous HCl and the product was extracted with EtOAc (2x). The combined organic extracts were dried over MgSO₄ and evaporated to give an oil which was purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) to give the product as an oil (2.0g, 68%). R_f 0.20 (CH₂Cl₂/MeOH 9/5, v/v). MS m/z 269 (MH).

(b) 3.4-Dimethoxv-2-iodo-6-[l-(5-methanesulfonamido-1.2.3.4-tetrahvdroisoquinol-2yDethvlideneaminolbenzonitrile

The subtitle compound was prepared by the method of Example 1 (f) from the product of step (a) and the compound of Example 1(e). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (98/2, v/v) to afford the subtitle compound (93%) as a colourless foam. Rf 0.30 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 555 (MH).

AP/P/ 9 8/01175 (c) 3.4-Dimethoxv-6-jT -(5-methanesulfonamido-1.2.3.4-tetrahvdroisoquinol-2vl)ethvlideneaminol-2-(2-pvrimidyl)benzonitrile

The subtitle compound was prepared by the method of Example 5 from the product of step (b) and 2-(tri-n-butylstannyl)pyrimidine. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (96/4, v/v) to give the subtitle compound (32%) as a foam. R_f 0.11 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 507 (MH*).

APO 00 8 1 9 (d) 4-Amino-6 J-dimethoxy-2-(5-methanesulfonamido-1.2.3,4-tetrahvdroisoquinol-2vl)-5-(2-pvrimidvl)quinoline A solution of zinc chloride in THF (0.5M, 10,6ml, 5.3mmol) was added to a solution of the product of step (c) (180mg, 0.36mmol) in THF (5ml) and the reaction mixture was heated to reflux for 70h, after which time a further portion of zinc chloride in THF (0.5M, 3.5ml) was added and heating continued at reflux for a further 7h. After cooling, the reaction mixture was partitioned between CH₂C1₂ and a solution of EDTA in 2N aqueous NaOH, the organic layer was washed with saturated brine and dried over MgSO₄. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the title compound as a colourless solid (38mg, 21%). Rf 0.28 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 507 (ΜΗ*). Ή NMR (CDC1₃) δ: 2.83 (2H, m), 3.00 (3H, s), 3.64 (3H, s), 3.96 (5H, m), 4.46 (2H, bs), 4.77 (2H, s), 6.07 (IH, s), 7.0-7.2 (IH, bs), 7.06 (IH, s), 7.15 (IH, t), 7.22 (IH, d), 7.43 (IH, t), 7.50 (IH, bs), 8.96 (2H, d).

Example 23

4-Amino-6-ethoxv-2-(5-methanesulfonamido-1.2.3-4-tetrahvdroisoquinoI-2-vl)-7methoxv-5-(2-pvridvl)quinoline (a) 3-Hydroxv-2-iodo-4-methoxv-6-nitrobenzonitrile J 20 To a solution of the compound of Example 1(d) (lO.Og, 30mmol) in collidine (100ml) was / λ added lithium iodide (4.0g, 30mmol) and the reaction was stirred at room temperature for

18h followed by heating at 100°C for 1.5h. After cooling, the reaction mixture was partitioned between 2N aqueous NaOH and EtOAc, the layers were separated and the product was extracted with 3 further EtOAc washes. The combined organic layers were washed with 2N HCl (2x), dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH (97/3, v/v) to afford the subtitle compound as a colourless solid (6.96g, 73%). Rf 0.16 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 338 (MNHf).

(b) 3-Ethoxv-2-iodo-4-methoxv-6-nitrobenzonitrile

To a solution of the product of step (a) (6.95g, 21.7mmol) and bromoethane (1.78ml,

23.8mmol) in DMF (70ml) was added potassium carbonate (4.49g, 32.5mmol) and the

AP/P/ 98/01175

APO 0 0 8 1 9 reaction was heated to 60°C for 18h. After cooling, the reaction mixture was partitioned between 2N aqueous HCI and EtOAc, the organic layer was separated, washed with H₂O, dried over MgSO₄ and evaporated. The crude product was purified on silica gel, eluting with CH₂Cl₂/MeOH (97/3, v/v) to afford the subtitle compound as a colourless solid (2.94g, 39%). Rf 0.68 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 366 (MNH₄ ⁺).

(c) 6-Amino-3-ethoxv-2-iodo-4-methoxybenzonitrile

The subtitle compound was prepared by the method of Example 1(e) from the product of step (b). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (98/2, v/v) to afford the subtitle compound (72%) as a colourless solid. Rf 0.11 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 336 (MNH₄ ⁺).

(d) 3-Ethoxv-2-iodo-6-il-(5-methanesulfonamido-1.2-3.4-tetrahvdroisoquinol-2vl)ethvlideneaminol-4-methoxybenzonitrile

The subtitle compound was prepared by the method of Example 1 (f) from the product of step (c) and the compound of Example 22(a). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (98/2, v/v) to afford the subtitle compound (53%) as a colourless foam. Rf 0.14 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 569 (MH⁺).

AP/P/ 9 8/01

J 20 (e) 3-Ethoxv-6-il-(5-methanesulfonamido-1.2.3.4-tetrahydroisoquinol-2vl)ethvlideneamino1-4-methoxy-2-(2-pvridyl)benzonitriIe

The subtitle compound was prepared by the method of Example 5 from the product of step (d) and 2-(tri-n-butylstannyl)pyridine. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (94/6, v/v) to give the subtitle compound (54%) as a foam. Rf 0.14 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 520 (MH⁺).

(f) 4-Ammo-6-ethoxv-2-(5-methanesulfonamido-1.2.3,4-tetrahydroisoquinol-2-vl)-7methoxv-5-(2-pyridvl)quinoline

A solution of the product of step (e) (1.14g, 2.19mmol) in THF (10ml) was cooled to 30 78°C and treated with a solution of lithium diisopropylamide in cyclohexane (1.5M, 4.4ml,

6.6mmol). The reaction was then allowed to reach rooInTemperature over 1 In partitioned «GaRWOFPAiHM between EtOAc and H₂O and the aqueous layer extracts wim of EtOAc.

-8 JAN19S RO. BOTOV

CAO··'··· ‘

APO 0 0 8 1 9

The combined organic layers were washed with brine, dried over MgSO₄ and evaporated under reduced pressure. Purification on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃(93/7/1, v/v) followed by trituration with ether afforded the title compound as a colourless foam (510mg, 45%). Rf 0.23 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 520 (MH⁺).

Ή NMR (CDC1₃) δ: 0.92 (3H, t), 2.84 (2H, m), 3.00 (3H, s), 3.82 (4H, m), 3.98 (3H, s), 4.22 (2H, q), 4.77 (2H, s), 5.93' (IH, s), 7.07 (IH, m), 7.17 (IH, t), 7.20-7.35 (2H, m), 7.40 (IH, t), 7.50 (IH, d), 7.81 (IH, t), 7.46 (IH, d), 8.77 (IH, d). Found: C,60.32; H,5.66; N,12.60 C₂₇H₂₉N₅O₄S O.25.CH₂C1₂ requires C,60.51; H,5.50; N,12.95%.

Example 24

4-Amino-6.7-dimethoxy-5-(2-pyridvl)-2-(5.6.7.8-tetrahvdro-1.6-naphthyrid-6-vl)quinoline (a) 6-Acetyl-5.6.7.8-tetrahydro-1.6-naphthvridine

To a solution of 5,6,7,8-tetrahydro-l,6-naphthyridine (4.9g, 36.5mmol) in CH₂C1₂ at 0°C 15 was added triethylamine (6.1ml, 43.8mmol) and acetyl chloride (3.11ml, 43.8mmol) dropwise and the reaction was allowed to warm to room temperature and stirred for a further 18h. The reaction mixture was partitioned between H,0 and CH₂C1₂, the layers were separated and the aqueous layer was extracted twice more with CH₂C1₂. The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure to afford a residue which was purified on silica gel, eluting with EtOAc/MeOH/0.88NH₃(95/5/1, v/v). This afforded the subtitle compound (58%) as an oil. Rf 0.60 (CH₂Cl₂/MeOH/0.88NH₃ 84/14/2, v/v). Ή NMR (CDC1₃) δ: 2.15 (3H, s), 3.04 (2H, m), 3.75 and 3.90 (2H, 2xm), 4.60 and 4.70 (2H, 2xs), 7.10 (IH, m), 7.42 (IH, m), 8.42 (IH, m).

(b) 3.4-Dimethoxv-2-iodo-6-[T-f5.6.7.8-tetrahvdro-1.6-naphthvrid-6' vDethvlideneaminolbenzonitrile

The subtitle compound was prepared by the method of Example 1 (f) from the product of step (a) and the compound of Example 1(e). The product was purified on silica gel, eluting with EtOAc/MeOH (95/5, v/v) to afford the subtitle compound (80%) as a pale yellow solid. Rf 0.58 (CH₂Cl₂/MeOH/0.88NH₃ 92/7/1, v/v). MS m/z 463 (MH*).

AP/P/ 9 8/01175

AP Ο Ο Ο 8 1 9 (c) 3,4-Dimethoxv-2-(2-pvridyl~)-6- Γ 1-(5.6,7,8-tetrahvdro-1,6-naphthyrid-6vDethylideneaminolbenzonitrile

The subtitle compound was prepared by the method of Example 5 from the product of step (b) and 2-(tri-n-butylstannyl)pyridine. The product was purified on silica gel, eluting with

EtOAc/MeOH/0.88NH₃ (95/5/1, v/v) to give the subtitle compound (51%) as a foam. Rf 0.26 (EtOAc/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 414 (MH⁺).

(d) 4-Amino-6.7-dimethoxv-5-(2-pyridvl)-2-(5.6.7.8-tetrahvdro-l,6-naphthyrid-6yl)quinoline

The title compound was prepared by the method of Example 23(f) from the product of step (c). The product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (96/3.5/0.5, v/v) followed by trituration with ether to afford the title compound (22%) as a light brown solid. Rf 0.31 (CH₂Cl₂/MeOH/0.88NH₃ 92/7/1, v/v). MS m/z 414 (MH⁺). Ή

NMR (CDC1₃) δ: 3.13 (2H, m), 3.52 (3H, s), 3.82 (2H, bs), 3.98 (5H, m), 4.83 (2H, s), 5.98 (1H, s), 7.13 (1H, m), 7.22 (1H, bs), 7.38 (1H, m), 7.48 (1H, d), 7.53 (1H, m), 7.80 (1H, m), 8.43 (ΙΗ', d), 8.76 (1H, d). Found: C,67.74; H,6.26; N,15.43 C₂₄H₂₃N₅O₂S 0.4.ether Ο.ό.Η,Ο requires C,67.86; H,6.07; N, 15.33%.

Example 25

AP/P/ 98/01175

4-Amino-6.7-dimethoxv-5-(2-pvrimidyl)-2-(5,6.7.8-tetrahvdro-l,6-naphthvrid-6-

vDquinoline (a) 3.4-Dimethoxv-2-pvrimidyl-6-ll-(5.6.7.8-tetrahvdro-1.6-naphthvrid-6vDethylideneaminolbenzonitrile

The subtitle compound was prepared by the method of Example 5 from the product of Example 24(b) and 2-(tri-n-butylstannyl)pyrimidine. The product was purified on silica ' gel, eluting with CH₂Cl₂/MeOH (98/2, v/v) to give the subtitle compound as a foam (75%). RfO.21 (ether). MS m/z 415 (MH⁺).

(b) 4-Amino-6.7-dimethoxy-S-(2-pvrimidvl)-2-(5.6.7.8-tetrahydro-1.6-naphthyrid-6vDquinoline

APO00819

Potassium hydroxide powder (72mg, 1,29mmol) was added to a solution of the product of step (a) (530mg, 1,28mmol) in DMSO (5ml). The reaction mixture was heated to 95°C for 45min. After cooling the reaction mixture was poured into citric acid and basified with 2N aqueous NaOH. The product was then extracted with EtOAc (x4). The combined organic layers were washed with H₂O, saturated brine and dried over MgSO₄. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH/0.88NH₃ (96/3.5/0.5, v/v). The product was triturated with ether to afford the title compound as an orange solid (91 mg, 17%). Rf 0.11 CH₂Cl₂/MeOH (95/5, v/v). MS m/z 415 ( MH⁺) Ή NMR (CDC1₃), δ: 3.10 (2H, t), 3.69 (3H, s), 3.79 (2H, s), 4.00 (2H, m), 4.05 ( 3H, s), 4.82 (2H, s), 6.01 (1H, s), 7.05 (1H,

m), 7.40 (1H, t), 7.50 (1H, m), 8.40 (1H, m), 8.90 (2H, m).

-J

Example 26

4-Amino-7-methoxv-2-i4-(4-morpholinecarbonvl)-1.4-diazepan-l-vll-5-(2-pvridvl)-6(2.2.2-trifluoroethoxv)quinoline (aj 3-Hydroxv-4-methoxv benzoic acid, methvl ester

To a suspension of 3-hydroxy-4-methoxv benzoic acid (33.63g, 0.2mol) in MeOH (500ml) was added concentrated sulphuric acid (25ml) and the reaction mixture was heated to reflux for 2h. On cooling the reaction mixture was concentrated under reduced pressure to

A 20 100 ml and the residue was extracted with EtOAc. The organic layer was washed sequentially with H,0 (x2), saturated aqueous sodium bicarbonate and saturated brine, dried over MgSO„ and evaporated under reduced pressure, to afford the subtitle compound as colourless crystals (33.Og, 91%). Rf 0.59 (EtOAc). MS m/z 183 (MH⁺).

(b) 2.2.2-Trifluoroethvl trifluoromethanesulfonate.

To a solution of trifluoromethanesulfonic anhydride (80.4g, 0.3mol) in CH,C1₂ (50ml) was added a mixture of 2,2,2-trifluoroethanol (28.Og, 0.28mol) and triethylamine (29.3g, 0.29mol) at -40°C dropwise over 45min. After addition, the reaction mixture was warmed to room temperature, washed sequentially with H₂O and saturated aqueous sodium bicarbonate and then dried over MgSO₄. This afforded the subtitle compound as a solution in CH₂C1₂ which was used immediately in the next step.

AP/P/ 9 8/01 175

AP U ΰ Ο 8 1 9 (c) 4-Methoxv-3-(2.2.2-trifiuoroethoxy)benzoic acid, methvl ester

To a solution of the product of step (a) (33.Og, 0.181mol) in a mixture of potassium carbonate (41.4g, 0.3mol) and DMF (100ml) was added a solution of the product obtained from step (b) (65.Og, 0.28mol). The reaction mixture was stirred at room temperature for

18h after which time the reaction mixture was evaporated under reduced pressure. The residue was partitioned between ether and H₂O, the organic layer was washed with saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The crude product was triturated with hexane to afford the subtitle compound as a colourless solid (42.55g, 93% over 2 steps). Rf 0.47 (CH₂C1₂). MS m/z 265 (MH⁺).

/„0 (d) 4-Methoxv-3-(2.2.2-trifluoroethoxy)benzoic acid

To a solution of the product of step (c) (42.25g, 0.16mol) in MeOH was added 2N aqueous NaOH (160ml, 0.32mol). The reaction mixture was stirred at room temperature for 3h and then at 50°C for lh. After cooling the reaction mixture was evaporated under reduced pressure. The residue was partitioned between EtOAc and 2N HCI, the organic layer dried over MgSO₄ and evaporated under reduced pressure to afford the subtitle compound as a colourless solid (40.4g, 100%). R_f0.13 (hexane/EtOAc 1/1, v/v). MS m/z 251 (MH⁺).

(e) 4.4-Dimethvl-2-r4-methoxv-3-(2.2.2-trifluoroethoxy)phenvll-A²-oxazoline

To a suspension of the product of step (d) (40.0g, 0.16mol) in CH₂C1₂ (200ml) and DMF Os (0.5ml) was added oxalyl chloride (40.6g, 0.32mol) at 0°C over a period of 15min. The reaction mixture was stirred at 0°C for 30min, warmed to room temperature over 1.5h and evaporated under reduced pressure. The residue was then redissolved in CH₂C1₂ (300ml) and added over 15min to a solution of 2-amino-2-methylpropanol (17.8g, 0.2mol) and triethylamine (20.2g, 0.2mol) in CH₂C1₂ (100ml) at 0°C. The reaction mixture was then stirred at room temperature for 30min, washed sequentially with 5% citric acid and dilute aqueous sodium bicarbonate, dried over MgSO₄ and evaporated under reduced pressure to a volume of 200ml. Thionyl chloride (21.4g, 0.18mol) was added dropwise to the solution and the resulting mixture was stirred for lh. The product was then extracted with H₂O followed by 0.5N aqueous NaOH. The combined aqueous phases were basified with 2N aqueous NaOH and the product extracted with CH₂C1₂ (x2). The combined organic phases were dried over MgSO₄ and evaporated under reduced pressure to afford a batch of crude

AP/P/ 9 8/01 175 >·' 20 a

ΑΡ ϋ ο Ο 8 1 9 >·' 20 a product. The original organic extract was then shaken with 2N aqueous NaOH and the product extracted with CH₂C1₂ (x3), the combined organic extracts were dried over MgSO₄and evaporated under reduced pressure. The residue was redissolved in ethereal HCI (150ml), the resulting white solid was filtered off, rebasified with 2N aqueous NaOH and extracted with CH₂C1₂ (x3). The combined organic extracts were dried over MgSO₄ and evaporated under reduced pressure to afford a second batch of crude product. The combined crude products were purified on silica gel eluting with CH₂Cl₂/MeOH (95/5, v/v) to afford the subtitle compound as a colourless solid (38.8g, 80%). Rf0.54 (EtOAc). MS m/z 304 (MHJ.

(f) 4.4-Dimethvl-2-i2-iodo-4-methoxv-3-(2.2.2-trifluoroethoxv)phenvl1-A²-oxazoline The subtitle compound was prepared by the method of Example 1 (b) from the product of step (e). The crude product was purified on silica gel eluting with EtOAc/hexane (60/40, v/v). The product was then triturated with ether to afford the subtitle compound as an orange solid (53%). Rf 0.27 (ether/hexane 1/3, v/v). MS m/z 430 (MHJ.

(g) 2-lodo-4-methoxv-3-('2.2.2-trifluoroethoxv)benzonitrile

The subtitle compound was prepared by the method of Example 1(c) from the product of step (f). The crude product was triturated with hexane/ether (60/40, v/v) to afford the subtitle compound as a colourless solid (96%). Rf 0.5 (EtOAc/hexane 1/1, v/v). MS m/z 358 (MHJ.

(h) 2-Iodo-4-methoxv-6-nitro-3-('2.2.2-trifluoroethoxv)benzonitrile

The subtitle compound was prepared by the method of Example 1 (d) from the product of step (g). The crude brown solid was triturated with ether to afford the subtitle compound (72%). Rf 0.25 (hexane/EtOAc 2/1, v/v). MS m/z 403 (MHJ.

(i) 6-Amino-2-iodo-4-methoxv-3-f2.2.2-trifluoroethoxy)benzonitrile

The subtitle compound was prepared by the method of Example 1 (e) from the product of step (h). The crude product was washed though a plug of silica gel to afford the subtitle compound as an orange solid (70%). Rf 0.74 (CH₂Cl₂/MJQIiJ'e'.SANH· 73/ //1, v/v)J MS

AP/P/ 9 8/01175 m/z 373 (MHJ.

»8 JAN1GP ! po.eoxcy

CAUSEWAY, TAPO 0 0 8 1 9 (j) 2-Iodo-4-methoxv-6-{ l-r4-(4-morpholinecarbonvP-l .4-diazepan-lyl1ethylideneamino|-3-f2.2-2-trifluoroethoxy)benzonitrile

The subtitle compound was prepared by the method of Example 1 (f) from the product of 5 step (i) and Intermediate 4. The crude product was purified on silica gel eluting with

CH₂Cl₂/MeOH (90/10, v/v) to' afford the subtitle compound as an orange oil which was recrystallised from EtOAc to yield a colourless solid (64%). Rf 0.12 (EtOAc). MS m/z 610 (MHJ.

(k) 4-Methoxv-6-{l-i4-f4-morpholinecarbonyl)-1.4-diazepaii-l-vnethvlideneamino)2-('2-pyridvP)-3-f2.2,2-trifluoroethoxy)benzonitrile

The subtitle compound was prepared by the method of Example 5 from the product of step (j) and 2-(tri-n-butylstannyl)pyridine. The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH (96/4, v/v) to afford the subtitle compound as a yellow foam (91%). Rf

0.43 (CH₂Cl,/MeOH/0.88NH₃ 93/7/1, v/v). MS m/z 561 (MHJ.

(1) 4-Amino-7-methoxv-2-[~4-(4-morpholinecarbonvl)-1.4-diazepan-l-yll-5-(2pvridvl)-6-(2.2.2-trifluoroethoxv)quinoline

To a solution of the product of step (k) (0.56g, lmmol) in THF (10ml) was added freshly 20 prepared lithium diisopropylamide (4ml, 2mmol) at -20°C. The reaction mixture was then warmed to room temperature slowly and stirred for 20min, after which time it was quenched with H₂O and poured into EtOAc. The organic layer was then washed with 2N aqueous NaOH followed by saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel eluting with

CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the title compound as a brown foam (0.39g, 70%). Rf 0.37 (CH₂Cl₂/MeOH/0.88NH₃ 93/7/1, v/v). MS m/z 561 (MHJ. Ή ' NMR (CDClj δ: 2.00 (2H, m), 3.10 (5H, m), 3.30 (2H, m), 3.50-3.90 (10H, m), 3.95 (3H,

s), 4.00 (3H, s), 5.80 (lH,s), 7.10 (lH,bs), 7.39 (IH, m), 7.45 (IH, d), 7.80 (IH, m), 8.70 (IH, d). Found C,51.49; H,5.72; N,14.35; C₂₇H₃₁F₃N₆O₄ 0.33.EtOAc requires C, 51.69;

H,5.71 ;N,14.35%.

AP/P/ 9 8/01 175

Example 27

AP Ο Ο Ο 819

4-Amino-7-methoxv-5-f2-nvrimidinvl)-2-f5,6,7.8-tetrahydro-1.6-naphthyrid-6-vi)-6(2.2.2-trifluoroethoxv)qumoline (a) 2-Iodo-4-methoxv-6-[l-(5.6.7.8-tetrahvdro-1.6-nar>hthvrid-6-yl)ethvlideneamino'l5 3-(2.2.2-trifluoroethoxv)benzonitrile

The subtitle compound was prepared by the method of Example 1 (f) from the product of Example 26(i) and the compound of Example 24(a). The crude product was purified on silica gel eluting with EtOAc/MeOH (80/20, v/v) to afford the subtitle compound as a colourless foam (70%). Rf 0.63 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 531 (MH⁺).

(b) 4-Methoxv-2-(2-pvrimidvI)-6-ri-(5.6.7.8-tetrahvdro-1.6-naphthvrid-6vl)ethvlideneamino1-3-(2.2.2-trifluoroethoxy)benzonitrile

The subtitle compound was prepared by the method of Example 5 from the product of step 15 (a) and 2-(tri-n-butyIstannyl)pyrimidine. The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH/0.88NH₃ (94/6/1, v/v) to afford the subtitle compound as a colourless solid (49%). Rf 0.29 (CH₂Cl₂/MeOH/0.88NH₃ 93/7/1, v/v). MS m/z 483 (MH’).

(c) 4-Amino-7-methoxv-5-(2-pvrimidinyl)-2-(5.6.7.8-tetrahvdro-1.6-naphthvrid-6-vl)20 6-(2.2.2-trifluoroethoxv)quinoline

The title compound was prepared by the method of Example 25(b) from the product of step (b). The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH (95/5, v/v) to afford the title compound as a foam (8%). Rf 0.07 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 483 (MH⁺). Ή NMR (CDC1₃) δ: 3.18 (2H, m), 3.80 (2H, bs), 4.00 (5H, m), 4.30 (2H, m), 4.90 (2H, s), 6.00 (IH, s), 7.10 (IH, m), 7.30 (IH, s), 7.40 (IH, m), 7.50 (IH, d), 8.40 (IH, d),

8.90 (2H, d).

AP/P/ 9 8/01175

Example 28

4-Amino-6.7-dimethoxv-2-i4-(4-morpholinecarbonvl)-1.4-diazepan-l-vl1-5-(oxazol-2-


	k ·*	REGiSRAR OF PATENTS !
-	j	AND TRADEMARK’S ‘
	ί	*8 JANV · ’
		PO. BOX O ' . 7
	L	CAUSEWAY, 27 77// '

AP000819

To a solution of oxazole (276mg, 4mmol) in THF (15ml) was added n-butyllithium (1.6M in hexane, 2.75ml, 4.4mmol) dropwise at -78°C under a nitrogen atmosphere. The reaction mixture was stirred at -78°C for 20min, zinc chloride solution (1.0M in ether, 12ml, 12mmol) was then added to the reaction mixture and the resulting solution was warmed to room temperature. The compound of Example 1(f) (1.05g, 1.94mmol) was added followed by tetrakis(triphenylphosphine)palladium (200mg). The reaction mixture was heated at reflux for 3h. This was followed by the addition of a further portion of oxazole zincate, prepared as above using oxazole (552mg, 8mmol), n-butyllithium (1.6M in hexane, 5.5ml, 8.8mmol) and zinc chloride solution (1.0M in ether, 24ml, 24mmol) followed by tetrakis10 (triphenylphosphine)palladium (lOOmg). The reaction mixture was refluxed for 4h after which time copper(I) iodide (lOOmg) was added. The resulting mixture was refluxed for 24h. The cooled reaction mixture was poured into EtOAc and washed with aqueous EDTA solution, basified with 2N aqueous NaOH and the organic layer was separated, washed with saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH/0.88NH₃ (92/8/1, v/v) to afford the title compound (87mg, 9%). RfO.46 (CH₂Cl₂/MeOH/0.88NH₃ 93/7/1, v/v). MS m/z 483 (MH⁺). Ή NMR (CDC1₃) δ: 2.10 (2H, m), 3.10 (4H, m), 3.35 (2H, m), 3.50-3.90 (12H, several peaks), 3.95 (2H, m), 4.00 (3H, s), 4.20 (IH, bs), 5.85 (IH, s), 7.35 (IH, s), 7.70 (lH,m), 7.90 (lH,s).

Example 29

4-Amino-6.7-dimethoxv-2-(2-methvl-5.6.7,8-tetrahydro-l,6-naphthyrid-6-vl)-5-(2pyridvlfgumoline

AP/P/ 9 8/01 175 (a) 6-Acetyl-2-methyl-5.6.7.8-tetrahvdro-1.6-naphthvridine

To a solution of 2-methyl-5,6,7,8-tetrahydro-l,6-naphthyridine [Shiozawa et al., Chem. Pharm. Bull., 32, 2522, (1984)] (2.73g, 0.0184mol) in CH₂C1₂ (30ml) and triethylamine (5.1ml, 0.0368mol) was added acetyl chloride (1.57ml, 0.0221mol) at 0°C. The reaction mixture was stirred for 24h at room temperature, after which time the reaction mixture was washed sequentially with saturated aqueous sodium bicarbonate, H₂O, saturated brine, dried over MgSO₄ and evaporated under reduced pressure to afford the subtitle compound (3.27g, 93%). Rf 0.5 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 191 (MH⁺).

AP Ο Ο Ο 8 1 9 (b) 3.4-Dimethoxy-2-iodo-6-ri-f2-methvl-5.6.7.8-tetrahvdro-1.6-naphthvrid-6vDethvlideneaminolbenzonitrile

The subtitle compound was prepared by the method of Example 1 (f) from the product of 5 step (a) and the compound of Example 1(e). The crude product was purified on silica gel eluting with EtOAc/hexane (96/4, v/v) to afford the subtitle compound as a foam (87%). Rf

0.42 (EtOAc). MS m/z 477 (MH⁺).

(c) 3,4-Dimethoxv-6-[l-(2-methvI-5.6.7.8-tetrahydro-1.6-naphthvrid-610 vf)ethylideneamino1-2-(2-pvridyl)benzonitrile

The subtitle compound was prepared by the method of Example 5 from the compound of step (b) and 2-(tri-n-butylstannyl)pyridine. The crude product was purified on silica gel eluting with EtOAc/MeOH (97/3, v/v) to afford the subtitle compound as a foam (25%). Rf 0.29 (EtOAc). MS m/z 428 (MH*).

(d) 4-Amino-6.7-dimethoxv-2-(2-methvl-5.6.7.8-tetrahvdro-1.6-naphthvrid-6-vl)-5-(2pyridvl)quinoline

The title compound was prepared by the method of Example 26(1) from the product of step (c). The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH/0.88NH₃ ) 20 (93/7/1, v/v) to afford the title compound as a foam (10%). Rf 0.25 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 233 (ΜΗ*). Ή NMR (CDC1₃) δ: 1.30 (2H, bs), 2.50 (3H, s), 3.10 (2H, m) 3.59 (3H, s), 3.85 (2H, m), 3.95-4.00 (6H, m), 4.80 (2H, s), 6.00 (IH, s), 7.00 (IH, d), 7.20 (IH, s), 7.40 (IH, m), 7.45 (IH, m), 7.80 (IH, m), 8.75 (IH, m).

Example 30 ' 4-Amino-6.7-dimethoxv-2-(5-methoxy-1.2.3.4-tetrahvdroisoquinol-2-yl)-5-(2pvridvDquinoline

AP/P/ 9 8/01175 (a) 5-Methoxvisoquinoline

To a solution of 5-hydroxyisoquinoline (lOg, 69mmol) in MeOH (100ml) was added a solution of sodium methoxide in methanol (30% by weight, 13.8ml, 72.4mmol) followed

AP Ο Ο Ο 8 1 9 by phenyltrimethylammonium chloride (12.4g, 72.4mmol). The reaction mixture was stirred at room temperature for 2h, after which time it was filtered and the filtrate evaporated under reduced pressure to afford an oil which was dissolved in DMF (50ml). The reaction mixture was refluxed for 2h after which time the reaction mixture was evaporated under reduced pressure. The resulting oil was partitioned between CH₂C1, and IN aqueous NaOH, the organic layer was washed twice with IN aqueous NaOH, dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel eluting with EtOAc/hexane (1/1, v/v) to afford the subtitle compound as a yellow oil (6.1 lg, 56%). Ή NMR (CDC1₃) δ: 4.05 (3H, s), 7.00 (IH, d), 7.55 (2H, m), 8.02 (IH,

d), 8.55 (IH, d), 9.22 (IH, s).

(b) 5-Methoxy-1.2.3.4-tetrahydroisoquinoline

To a solution of the product of step (a) (6.1 lg, 384mmol) in EtOH (200ml) was added platinum oxide (0.61 lg) followed by concentrated HCI (3.2ml, 38.4mmol). The reaction mixture was hydrogenated at 345kPa (50p.s.i.) at room temperature for 4h after which time the catalyst was filtered off and washed with EtOH. The filtrate was evaporated under reduced pressure to afford the subtitle compound as a colourless solid (7.27g, 95%). *H NMR (D₆-DMSO) δ: 2.80 (2H, m), 3.35 (2H, m), 3.80 (3H, s), 4.20 (2H, s), 6.80 (IH, d), 6.90 (IH, d), 7.20 (IH, t), 9.45 (2H, bs).

(c) 2-Acetvl-5-methoxv-1.2.3.4-tetrahvdroisoquinoline

To a solution of the product of step (b) (6.26g, 31.4mmol) and triethylamine (9.6ml, 69.0mmol) in CH,C1₂ (150ml) was added acetyl chloride (2.7ml, 37.7mmol) at 0°C over a period of 15min. The reaction mixture was stirred at room temperature for 18h after which time the solution was washed sequentially with H₂O and saturated aqueous sodium bicarbonate solution, dried over MgSO₄ and evaporated under reduced pressure. The crude ' product was purified on silica gel eluting with EtOAc to afford the subtitle compound as an orange oil (6.07g, 94%). Rf 0.65 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/s 206 (MH⁺).

(d) 3.4-Dimethoxv-2-iodo-6-ll-(5-methoxy-1.2.3.4-tetrahvdroisoquinol-2vDethylideneaminolbenzonitrile

AP/P/ 9 8/01 175

APO Ο ο 8 1 9

The subtitle compound was prepared by the method of Example 1 (f) from the product of step (c) and the compound of Example 1(e). The crude product was purified on silica gel eluting with CH₂C1₂ to afford the subtitle compound as orange crystals (69%). Rf 0.77 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 492 (MH⁺).

(e) 3 -4-Dimethoxv-6-r 1-(5 -methoxy-1.2.3,4-tetrahydroisoquinol-2yl)ethylideneamino~l-2-(2-pvridyl)benzonitrile

The subtitle compound was prepared by the method of Example 5 from the product of step (d) and 2-(tri-n-butylstannyl)pyridine. The crude product was purified on silica gel eluting with ether to afford the subtitle compound as a colourless solid (62%). Rf 0.73 (CH₂Cl₂/MeOH 90/10, v/v). MS m/z 443 (MH⁺).

(f) 4-Amino-6.7-dimethoxy-2-(5-methoxy-1.2.3.4-tetrahvdroisoquinol-2-vl)-5-(2pvridvDquinoline

The title compound was prepared by the method of Example 25(b) from the product of step (e) . The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH (95/5, v/v) to afford the title compound as a colourless solid (10%). Rf 0.5 (CH₂CD/MeOH 90/10, v/v). MS m/z 443 (ΜΗ*). Ή NMR (CDC1₃) δ: 2.90 (2H, t), 3.55 (3H, s), 3.75-3.90 (7H, m), 4.00 (3H, s), 4.79 (2H, s), 5.95 (IH, bs), 6.70 (IH, d), 6.85 (IH, d), 7.19 (IH, t), 7.25 (IH, s),

7.39 (IH, t), 7.45 (IH, d), 7.80 (IH, t), 8.75 (IH, d). Found C,68.58; H,5.93; N,12.66;

C₂₆H₂₆N₄O₃ 0.2.ether Ο.ό.Η,Ο requires C, 68.76; H,6.29; N,11.97%.

Example 31

4-Amino-6.7-dimethoxv-2-(6.7-dimethoxv-1.2.3.4-tetrahvdroisoquinol-2-vl)-5-(225 pvrimidvDquinoline '(a) 2-Acetvl-6.7-dimethoxv-1.2.3.4-tetrahvdroisoquinoline

The subtitle compound was prepared by the method of Example 30(c) from 6,7-dimethoxy1,2,3,4-tetrahydroisoquinoline. The crude product was purified on silica gel eluting with

EtOAc to afford the subtitle compound as a colourless solid (99%). Rf 0.15 (EtOAc). 'H

NMR (d₆-DMSO) δ: 2.05 (3H, s), 2.60-2.80 (2H, d), 3.55 (2H, m), 3.65 (6H, s), 4.25 (2H,

d), 6.70 (2h, d).

AP/P/ 9 8/01 175

AP Ο Ο Ο 8 1 9 (b) 3.4-Dimethoxv-6- Γ1 -(6,7 -dimethoxy-1.2.3.4-tetrahydroisoquinoi-2-vl)ethvlideneaminol-2-iodobenzon.itrile

The subtitle compound was prepared by the method of Example 1 (f) from the product of 5 step (a) and the compound of Example 1(e). The crude product was purified on silica gel eluting with CH₂C1₂ to afford the subtitle compound (71%). Rf 0.74 (EtOAc). MS m/z 522 (MH⁺).

(c) 3.4-Dimethoxy-6-ri-(6,7-dimethoxv-1.2.3.4-tetrahvdroisoquinol-2-vl)ethylidene10 amino1-2-(2-pvrimidvl)benzonitrile

The subtitle compound was prepared by the method of Example 5 from the product of step (b) and 2-(tri-n-butylstannyl)pyrimidine. Purification on silica gel afforded the subtitle compound (33%). Rf 0.38 (EtOAc). MS m/z 474 (MH⁺).

(d) 4-Amino-6-7-dimethoxv-2-(6.7-dimethoxv-1.2.3.4-tetrahydroisoquinol-2-yI)-5-(2pvrimidvl)quinoline

The title compound was prepared by the method of Example 26(1) from the product of step (c). The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH/0.88NH₃(95/5/0.5, v/v) to afford the title compound as a foam (29%). Rf 0.16 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 474 (MH⁺). Ή NMR (CDC1₃) δ: 2.90 (2H, m), 3.70 (5H, s), 3.90 (9H, m),

4.00 (3H, s), 4.75 (2H, s), 6.65 (IH, s), 6.75 (lH,s), 7.20 (IH, s), 7.40 (IH, t), 8.95 (2H, m).

AP/P/ 9 8/01 175

Example 32

4-Amino-6.7-dimethoxy-2-(6,7-dimethoxy-1,2.3.4-tetrahvdroisoquinol-2-vl)-5-(2-pyridvl)quinoline (a) 4-Ajnino-6.7-dimethoxy-2-(6.7-dimethoxv-1.2.3.4-tetrahvdroisoquinol-2-yl)-5iodoquinoline

The subtitle compound was prepared by the method of Example 1(g) from the compound of Example 31(b). The crude product was purified on silica gel eluting with EtOAc/hexane

AP 0 0 0 8 7 9 (1/1, v/v) then with EtOAc to afford the subtitle compound as an off-white solid (67%). Rf 0.5 (EtOAc). MS m/z 522 (MH⁺).

(b) 4-Amino-6.7-dimethoxv-2-(6.7-dimethoxv-1.2.3.4-tetrahvdroisoquinol-2-yl)-5-(25 pvridyDquinoline

The title compound was prepared by the method of Example 5 from the product of step (a) and 2-(tri-n-butylstannyl)pyridine. The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH/0.88NH₃ (95/5/0.5, v/v) to afford the title compound (20%). Rf 0.28 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 473 (MH⁺). Ή NMR (CDC1₃) δ: 2.85 (2H, t), 3.50 (3H,

s), 3.70-3.90 (10H, m), 4.00 (3H, s), 4.70 (2H, s), 5.95 (IH, s), 6.65 (1Ή, s), 6.70 (IH, s),

Vp 7.20 (IH, s), 7.35 (IH, t), 7.45 (IH, d), 7.80 (IH, d), 8.75 (IH, d).

Example 33

4-Amino-6.7-dimethoxv-2-r2-(4-morpholino)-5.6.7.8-tetrahvdro-1.6-naphthvrid-6-vn-515 (2-pyridyl)quinazoline

(a) 6-Benzvl-3.4.5.6.7.8-hexahydro-1.6-naphthvridin-2-one

To a solution of l-benzyl-4-piperidone (213g, 1.13mol) in toluene (700ml) was added pyrrolidine (190ml, 2.25mol), the reaction mixture was fitted with a Dean-Stark head and heated to 150°C for 18h. The reaction mixture was cooled and evaporated under reduced pressure, p-toluenesulphonic acid (4.0g, 0.022mol) was then added to the residue followed by acrylamide (160g, 2.25mol). The reaction mixture was heated with rapid stirring to 90°C for 1.5h and then for a further 2h at 120°C. The cooled mixture was then filtered and the solid obtained washed with acetone followed by ether. The mother liquors were combined, evaporated under reduced pressure and the residue partitioned between EtOAc and E1₂O. The organic layer was separated, dried over MgSO₄ and evaporated under reduced pressure to afford a further batch of solid. The solids were combined and heated to reflux with 4-toluenesulphonic acid (lOg, 0.056mol) in dioxane (400ml) for 18h. On cooling, a colourless crystalline product was formed which was filtered and washed with EtOAc to afford the subtitle compound as colourless crystals (176g, 65%). Rf 0.1 (EtOAc).

£ I 10/86 IdldM

Ή NMR (CDC1₃) δ: 2.20 (4H, d), 2.50 (2H. t), 2.70 (2H, s), 3'-00 ^Η^'3;ύΰ··(2Η; -sj.-T^O7 45 f_5Hm) I AEQlSfiWi OF PATENTS j

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CAUSEWAY,/ AP U Ο Ο 8 1 9 (b) 6-Benzyl-2-chloro-5.6.7,8-tetrahydro-1.6-naphthyri dine

To a stirred suspension of the product of step (a) (30g. 0.124mol) in toluene (400ml) was added phosphorous oxychloride (57.7ml, 0.619mol), followed by tetrachloro-l,4benzoquinone (31.98g, 0.13mol). The reaction mixture was refluxed under nitrogen for 18h after which time the toluene was evaporated under reduced pressure, the residue was then basified with 4N aqueous NaOH and the product extracted with ether (x3). The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel eluting with EtOAc to afford the subtitle compound as a solid (13.29g, 41%). R, 0.8 (EtOAc). MS m/z 259 (MH⁺).

(^c) 2-Chloro-5.6.7.8-tetrahydro-1.6-naphthyridine

To a stirred solution of the product of step (b) (13.28g, 0.0513mol) in toluene (150ml) was added 1-chloroethyl chloroformate (5.54ml, 0.0513mol) dropwise at 0°C. The reaction mixture was refluxed for 2h. On cooling the toluene was evaporated under reduced pressure and the residue partitioned between EtOAc/H,O, the organic layer was washed sequentially with IN HCI and saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The resulting residue was dissolved in MeOH (150ml) and refluxed for 3h after which time the reaction mixture was evaporated under reduced pressure and the residue was partitioned between CH₂C1₂ and 2N aqueous NaOH and the product extracted with CH₂C1₂ (x5). The combined organic layers were dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the subtitle compound (3.57g, 41%). Rf 0.25 (CH₂Cl₂/MeOH/0.88NH₃, 90/10/1, v/v). MS m/z 169 (MH⁺).

(d) 2-Chloro-6-diphenvlmethyl-5.6.7,8-tetrahydro-l,6-naphthvridine

To a solution of the product of step (c) (1.78g, 0.01 mol) and triethylamine (2.21ml, 0.016mol) in CH₂C1₂ (20ml) was added diphenylchloromethane (2.13ml, 0.012mol). The reaction mixture was stirred at room temperature for 20h and evaporated under reduced pressure. The residue was dissolved in DMA (20ml) and heated to 100°C for 18h and once cooled, the solution was diluted with CH₂C1₂ and washed sequentially with saturated aqueous sodium bicarbonate solution, H₂O and saturated brine then dried over MgSO₄.

AP/P/ 9 8/01 175

AP Ο Ο Ο 8 1 9

Evaporation under reduced pressure afforded the subtitle compound as a solid (l.Olg, 30%), Rf 0.7 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 335 (MH⁺).

(e) 6-Diphenvlmethvl-2-(4-morpholino)-5.6.7.8-tetrahvdro-1.6-naphthvridine

To a solution of morpholine (0.62ml, 7.17mmol) in THF (15ml) was added ethylmagnesium bromide (2.4ml, 7.17mmol) at 0°C, the reaction mixture was stirred for lh at room temperature after which time a solution of the product of step (d) (0.8g, 2.389mmol) in THF (15ml) was added followed by palladium(II) acetylacetonate (0.073g, 0.239mmol) and triphenylphosphine (0.125g, 0.478mmol) and the reaction mixture was heated to 60°C for 18h. On cooling the solution was partitioned between EtOAc and Vs#) saturated aqueous ammonium chloride solution, the organic layer was separated, washed sequentially with H₂O, saturated brine, dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH (97/3, v/v) to afford the subtitle compound (0.8lg, 88%). Rf 0.63 (CH₂Cl₂/MeOH 95/5, v/v). MS m/z 386 (MHQ.

(f) 2-(4-Morpholino)-5.6.7.8-tetrahvdro-1.6-naphthvridine

To a solution of the product of step (e) (0.8g, 2.08mmol) in MeOH/lN HCI (10/1, v/v, 33ml) was added 20% palladium hydroxide on carbon (0.2g). The reaction mixture was hydrogenated at 3451cPa (50p.s.i.) and 50°C for 56h after which time the catalyst was filtered off and washed with MeOH. The resulting solution was evaporated under reduced pressure and the residue partitioned between CH₂C1₂ and saturated aqueous sodium bicarbonate solution. The product was extracted with CH₂C1₂ (x8), the combined organic layers were dried over MgSO₄ and evaporated under reduced pressure. The crude product was purified on silica gel eluting with CH₂Cl₂/MeOH/0.88NH₃ (90/10/1, v/v) to afford the subtitle compound (0.13g, 28%). Rf 0.4 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z ' 220 (MH⁺).

(g) 4-Amino-6.7-dimethoxv-2-[2-(4-morpholino)-5.6.7.8-tetrahydro-1.6-naphthyrid-630 yll-5-(2-pvridvl)quinazoline

The title compound was prepared by the method of Example 12(b) from the compound of

Example 12(a) and the product of step (f). The crude product was purified on silica gel

AP/P/ 9 8/01175

AP Ο Ο Ο 8 1 9 eluting with CH₂Cl₂/MeOH (95/5, v/v) to afford the title compound as a colourless foam (29%). Rf 0.37 (CH₂Cl₂/MeOH/0.88NH₃ 90/10/1, v/v). MS m/z 450 (MH⁺). Ή NMR (CDCIj) δ: 1.30 (2H, s), 2.50 (3H, s), 3.10 (2H, m), 3.90-4.1(8H, several peaks), 4.80 (2H, s), 6.00 (lH,'s), 7.00 (IH, d), 7.24 (IH, s), 7.40 (2H, m), 7.45 (IH, d), 7.80 (IH, t), 8.75 (lH,m).

Example 34

4-Amino-6.7-dimethoxv-2-(5-methanesulfonamido-1.2.3.4-tetrahydroisoquinol-2-vl)-5-(2pyridyDquinoline (a) 3,4-Dimethoxv-6- Γ1 -(5-methanesulfonamido-1.2,3,4-tetrahydroisoquinol-2yl)ethvlideneamino1-2-f2-pyridyl)benzonitrile

The subtitle compqund was prepared by the method of Example 5 from the compound of Example 22(b) and 2-(tri-n-butylstannyl)pyridine. The product was purified on silica gel, eluting with CH₂Cl₂/MeOH (95/5, v/v) to give the subtitle compound (45%) as a foam. Rf 0.11 (CH₂Cl₂/MeOH 95/5, v/v).

(b) 4-Amino-6.7-dimethoxv-2-(5-methanesulfonamido-1.2,3.4-tetrahvdroisoquinol-2yl)-5-(2-pvridvl)quinoline hydrochloride

The title compound was prepared by the method of Example 23(f) from the product of step (a). The product was purified on silica gel, eluting with CH₂Cl,/MeOH/0.88NH₃ (90/10/1, v/v) followed by treatment with excess ethereal HCl to afford the title compound (10%) as a colourless solid. Rf 0.21 (CH₂Cl₂/MeOH/0.88NH₃ 93/7/1, v/v). MS m/z 506 (MH⁺). Ή NMR (d₆-DMSO) δ: 3.08 (2H, m), 3.48 (3H, s), 3.5-3.7 (5H, m), 3.80 (2H, m), 4.00 (3H,

m), 4.78 (2H, s), 6.00 (IH, bs), 6.19 (IH, s), 7.20 (IH, t), 7.28 (2H, m), 7.60 (2H, m), 7.90 (IH, s), 8.01 (IH, t), 8.77 (IH, d), 12.04 (IH, s). Found C,50.91; H,5.46; N,10.89;

' C₂₆H₂₈C1N₅O₄S O.8.CH₂C1₂ H₂O requires C, 51.26; H,5.07; N,11.15%.

AP/P/ 98/01175

Example 35

The compound of Example 28 was tested in the first screen described above /Contractile responses of human prostate') and found to have a pA, value of 9.2.

Claims

Claims:

1.

A compound of formula I,

Having now particularly described ant, ascertained my/oursaid invention J what manner the same is to be |K-ilbin.cd, 1/we declare that what 1/we claim is — wherein

R¹ represents C_M alkoxy optionally substituted by one or more fluorine, atoms;

R² represents H or C_M alkoxy optionally substituted by one or more fluorine atoms;

R³ represents a 5- or 6-membered heterocyclic ring containing at least one heteroatom

10 selected from N, O and S, the ring being optionally substituted by one or more groups selected from halogen, C_M alkoxy, C_M alkyl and CF₃;

R⁴ represents a 4-, 5-, 6-, or 7-membered heterocyclic ring containing at least one heteroatom selected from N, 0 and S, the ring being optionally fused.to a benzene ring or a

5- or 6-membered heterocyclic ring containing at least one heteroatom selected from N, O '15 and S, the ring system as a whole being optionally substituted by one or more groups independently selected from OH, C_M alkyl, C_M alkoxy, halogen, CONR^SR⁹, SO2NR⁸R⁹, (CH2)_bNR⁸R⁹ and NHSO₂(C_M alkyl), and when S is a member of the ring system, it may be substituted by one or two oxygen atoms;

R⁸ and R⁹ independently represent H or C_H alkyl, or together with the N atom to which

20 they are attached they may represent a 5- or 6-membered heterocyclic ring containing at least one heteroatom selected from N, 0 and S; b represents 0, 1, 2 or 3;

X represents CH or N; and . L is absent,

25 or represents a cyclic group of formula Ia, la

AP/P/ 9 8/01 175 (CH₂)„ / \ /^N\ (CH₂)_ro A in which N is attached to the 2-position of the quinoline or quinazoline ring;

A is absent or represents CO or SO₂;

APO Ο Ο 819

Ζ represents CH or N;

m represents 1 or 2, and in addition, when Z represents CH, it may represent 0; and ri represents 1, 2 or 3, provided that the sum of m and n is 2, 3, 4 or 5; or represents a chain of formula lb,

R⁷ lb (CH₂)_p a in which N is attached to the 2-position of the quinoline or quinazoline ring;

A’ and Z’ have the same significance as A and Z above, respectively;

R⁶ and R⁷ independently represent H or C_M alkyl; and p represents 1, 2 or 3, and in addition, when Z’ represents CH, it may represent 0;

or a pharmaceutically acceptable salt thereof.
2. A compound as claimed in claim 1, wherein R¹ and R² each represent methoxy.
3. A compound as claimed in claim 1 or claim 2, wherein R³ represents 2-pyridinyl or

2-pyrimidinyl.
4. A compound as claimed in any preceding claim, wherein X represents N.
5. A compound as claimed in any preceding claim, wherein L is absent.
6. A compound as claimed in any preceding claim, wherein R⁴ comprises a saturated

6-membered N-containing ring which is fused to a benzene or pyridine ring.
7. A compound as claimed in claim 6, wherein the benzene ring is substituted by NHSO₂(C_m alkyl).
8. A pharmaceutical formulation including a compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, in admixture with a pharmaceutically acceptable adjuvant, diluent or carrier.
9. A compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, for use as a pharmaceutical.
10. The use of a compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment of benign prostatic hyperplasia.

AP/P/ 9 8/01 175

APO 0 0 8 1 9
11. A method of treatment of benign prostatic hyperplasia, which comprises administration of a compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, to a patient in need of such treatment.
12. A process for the production of a compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, which comprises:

(a) when X represents CH, cyclizing a compound of formula X, x

in which R^M and L are as defined in claim 1;

(b) when A or A’ is present, and Z or Z’ represents N, reacting a compound of formula 10 XHIa or XHIb, as appropriate,

Xllla

R

I

NH <^CH2>_p XHIb in which R , R , R , X, m, n and p are as defined in claim 1, with a compound of formula XIV,

..A XIV

Lg

15 in which R⁴ is as defined in claim 1, A” represents CO or SO, and Lg represents a leaving group;

(c) reacting a compound of formula XVIII,

L_ ¥ ^R‘

XVIII

AP/P/ 9 8 / 0 117 5 in which R¹, R², R⁴, X and L are as defined in claim 1, with a compound of formula XIX,

R³-M XIX in which R³ is as defined in claim 1 and M represents substituted boron, zinc or tin, in the presence of a palladium catalyst;

(d) when X represents N, reacting a compound of formula XXII,

AP Ο Ο Ο 8 19

XXII in which R¹'³ are as defined in claim 1, with a compound of formula XXIIIa or XXIIIb, as appropriate,

R⁷K

P K XXIIIa

HN (CHA

R

I

N.

Z-^ ,/ A' (ch₂)_p ,R⁴

XXIIIb in which R⁴, R⁶, R⁷, A, A’, Ζ, Z’, m, n and p are as defined in claim 1;

(e) when A or A’ represents CO and R⁴ comprises a nucleophilic nitrogen atom in the heterocyclic ring attached to L, reacting a compound of formula XXVIIIa or XXVIIIb, as in which R¹'³, R⁶, R⁷, X, Ζ, Z’, m, n and p are as defined in claim 1, and Lg is a leaving group, with a compound of formula XXIX,

AP/P/ 9 8/01 175

HR^4a XXIX in which R^4a represents the groups defined by R⁴ in claim 1 which contain a nucleophilic nitrogen atom in the ring, this nucleophilic nitrogen atom being attached to H;

(f) conversion of a compound of formula I in which L represents a cyclic group of formula la, to a corresponding compound of formula I in which L represents a chain of formula lb in which R⁶ and R⁷ each represent H, by the action of a strong base;

(g) . when A or A’ is absent and Z or Z’ represents N, reacting a compound of formula XHIa or XHIb, as defined above, with a compound of formula XXX,

R⁴-Hal

XXX

AP Ο Ο Ο 8 1 9 in which R⁴ is as defined in claim 1 and Hal represents a halogen atom attached to the ring; or (h) when X represents N, L is absent and R⁴ comprises a nucleophilic nitrogen atom in 5 the heterocyclic ring attached to the quinoline or quinazoline ring, reacting a compound of formula XXII, as defined above, with a compound of formula XXIX, as defined above; and where desired or necessary converting the resulting compound of formula I into a pharmaceutically acceptable salt or vice versa.
13. Compounds of formulae X, XUIa, Xillb, XXII, XXVIIIa and XXVIIIb as defined 10 in claim 12.