MX2011004274A - Use of carboxamides on cultivated plants. - Google Patents

Use of carboxamides on cultivated plants.

Info

Publication number
MX2011004274A
MX2011004274A MX2011004274A MX2011004274A MX2011004274A MX 2011004274 A MX2011004274 A MX 2011004274A MX 2011004274 A MX2011004274 A MX 2011004274A MX 2011004274 A MX2011004274 A MX 2011004274A MX 2011004274 A MX2011004274 A MX 2011004274A
Authority
MX
Mexico
Prior art keywords
plant
plants
carboxamide
compound
health
Prior art date
Application number
MX2011004274A
Other languages
Spanish (es)
Other versions
MX343039B (en
Inventor
Markus Gewehr
Maarten Hendrik Stuiver
Dirk Voeste
Pilar Puente
Robert John Gladwin
Juergen Logemann
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of MX2011004274A publication Critical patent/MX2011004274A/en
Publication of MX343039B publication Critical patent/MX343039B/en

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/561,2-Diazoles; Hydrogenated 1,2-diazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/22Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/22Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
    • A01N37/24Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides containing at least one oxygen or sulfur atom being directly attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/24Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms
    • A01N43/32Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/781,3-Thiazoles; Hydrogenated 1,3-thiazoles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to a method of controlling pests and/or increasing the health of a plant as compared to a corresponding control plant by treating the cultivated plant, parts of a plant, seed, or their locus of growth with a carboxamide compound.

Description

USE OF CARBOXAMIDES IN CULTIVATED PLANTS Description The present application claims the priority benefit of the EP application 08167079. 6, filed on October 21, 2008. The entire content of each of the aforementioned applications is incorporated herein by reference.
The present invention relates to a method for controlling pests and / or increasing the health of a plant compared to a corresponding control plant by treating the cultivated plant, parts of a plant, seeds or its growth locus with a compound of carboxamide selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen ( N- [2- (1, 3-dimethylbutyl) -phenyl] -1,3-dimethyl-5-fluoro-1 H -pyrazole-4-carboxamide), fluopyram, sedaxane, sopirazam, pentiopyrad, benodanyl, carboxy, fenfuram , flutolanil, furametpir, mepronil, oxycarboxine and tifluzamide.
A typical problem that arises in the field of pest control is due to the need to reduce the dosage rates of the active ingredient in order to reduce or avoid unfavorable environmental or toxicological effects, while allowing effective control of pests. .
In relation to the present invention, the term pests comprises the harmful fungi. The term harmful fungi includes, but is not limited to, the following genera and species: Albugo spp. (white rust) in ornamentals, vegetables (for example, A. candida) and sunflowers (for example, A. tragopogonis); I would alternate spp. (Alternaria leaf spot) in vegetables, rapeseed (A. brassicola or brassicae), sugar beet. { A. tenuis), fruits, rice, soybeans, potatoes (for example, A. solani or A. alternata), tomatoes (for example, A. solani or A. alternata) and wheat; Aphanomyces spp. in sugar beet and vegetables; Ascochyta spp. in cereals and vegetables, for example, A. tritici (anthracnose) in wheat and A. hordei in barley; Bipolaris and Drechslera spp. (teleomorph: Cochliobolus spp.), for example, blight of the southern leaf (D. mayáis) or blight of the northern leaf. { B. zeicola) in corn, for example, spot (8. sorokiniana) in cereals and, for example, B. oryzae in rice and turf; Blumeria (formerly Erysiphe) graminis (powdery mildew) in cereals (for example, in wheat or barley); Botrytis cinerea (teleomorph: Botryotinia fuckeliana: gray mold) in fruits and berries (for example, strawberries), vegetables (for example, lettuce, carrots, celery and cabbages), rapeseed, flowers, vines, forest plants and wheat; Bremia lactucae (mildew) in lettuce; Ceratocystis (syn. Ophiostoma) spp. (rotting or wilting) on broadleaved and perennial trees, eg, C. ulmi (Dutch elm disease) on elms; Cercospora spp. (Cercospora leaf spots) in corn (for example, gray leaf spot: C. zeae-maydis), rice, sugar beet (for example, C. beticola), sugar cane, vegetables, coffee, soybeans ( for example, C. sojina or C. kikuchii) and rice; Cladosporium spp. in tomatoes (for example, C. fulvum: leaf mold) and cereals, for example, C. herbarum (black spike) in wheat; Claviceps purpurea (ergot) in cereals; Cochliobolus (anamorph: Helminthosporium of Bipolaris) spp. (leaf spots) in corn (C. carbonum), cereals (eg, C. sativus, anamorph: B. sorokiniana) and rice (eg, C. miyabeanus, anamorph: H. oryzae); Colletotrichum (teleomorph: Glomerella) spp. (anthracnose) in cotton (for example, C. gossypii), corn (for example, C. graminicola: stem rot anthracnose), soft fruits, potatoes (for example, C. coccodes, black spot), beans (for example, C. lindemuthianum) and soybean (for example, C. truncatum or C. gloeosporioides); Corticium spp., For example, C. sasakii (pod blight) in rice; Corynespora cassiicola (leaf spots) in soybeans and ornamentals; Cycloconium spp., For example, C. oleaginum in olive trees; Cylindrocarpon spp. (for example, fruit tree ulcer or deterioration of the young vine, teleomorph: Nectria or Neonectria spp.) in fruit trees, vines (for example, C. liriodendri, teleomorph: Neonectria liriodendri: Disease of the Blackfoot) and ornamentals; Dematophora (teleomorph: Rosellinia) necatrix (root rot and stem) in soybeans; Diaporthe spp., For example, D. phaseolorum (fall) in soybeans; Drechslera (syn. Helminthosporium, teleomorph: Pyrenophora) spp. in corn, cereals, such as barley (for example, D. teres, network spot) and wheat (for example, D. tritici-repentis: brown spot), rice and turf; Esca (acronecrosis (dieback), apoplexy) in vines, caused by Formitiporia (syn Phellinus) punctata, Mediterranean F., Phaeomoniella chlamydospora (early Phaeoacremonium chlamydosporum), Phaeoacremonium aleophilum and / or Botryosphaeria obtusa; Elsinoe spp. in pomo fruits (E. pyri), soft fruits (E. venenet: anthracnose) and vines (E. ampelina: anthracnose); Entyloma oryzae (leaf smudge) in rice; Epicoccum spp. (black mold) in wheat; Erysiphe spp. (powdery mildew) in sugar beets (E. betae), vegetables (eg, E. pisi), such as cucurbits (eg, E. cichoracearum), cabbage, rapeseed (eg, E. cruciferarum); Eutypa lata (Eutypa ulcer or acronecrosis, anamorph: Cytosporina lata, syn.Libertella blepharis) in fruit trees, vines and ornamental forests; Exserohilum (syn. Helminthosporium) spp. in corn (for example, £ turcicum); Fusarium (teleomorph: Gibberella) spp. (wilt, root rot or stem) in several plants, such as F. graminearum or F. culmorum (root rot, scab or top blight) in cereals (eg, wheat or barley), F. oxysporum in tomatoes, F. solani in soybeans and F. verticillioides in corn; Gaeumannomyces graminis (take-all) in cereals (for example, wheat or barley) and corn; Gibberella spp. in cereals (for example, G. zeae) and rice (for example, G. fujikuroi: Bakanae disease); Glomerella cingulata on vines, pomo fruits and other plants and G. gossypii on cotton; grain staining complexes in rice; Guignardia bidwellii (black rot) on vines; Gymnosporangium spp. in Rosaceae plants and junipers, for example, G. sabinae (rust) in pears; Helminthosporium spp. (syn.Drechslera, teleomorph: Cochliobolus) in corn, cereals and rice; Hemileia spp., For example, H. vastatrix (coffee leaf rust) in coffee; Isariopsis clavispora (syn. Cladosporium vitis) on vines; Macrophomina phaseolina (syn. Phaseoli) (root and stem rot) in soybeans and cotton; Microdochium (syn.Fusarium) nivale (pink snow mold) in cereals (for example, wheat or barley); Microsphaera diffusa (powdery mildew) in soybeans; Monilinia spp., For example, M. laxa, M. fructicola and M. fructigena (blight of flower and twig, brown rot) in stone fruits and other pink plants; Mycosphaerella spp. in cereals, bananas, soft fruits and nuts, such as, for example, M. graminicola (anamorph: Septoria tritici, Septoria spot) in wheat or M. fijiensis (black Sigatoka disease) in bananas; Peronospora spp. (mildew) in cabbage (for example, P. brassicae), rapeseed (for example, P. parasitic), onion (for example, P. destructor), tobacco (P. tabacina) and soybean (for example, P. manshurica); Phakopsora pachyrhizi and P. meibomiae (soybean rust) in soybeans; Phialophora spp. for example, in vines (for example, P. tracheiphila and P. tetraspora) and soybeans (for example, P. gregata: stem rot); Phoma lingam (root and stem rot) in rapeseed and cabbage and P. betae (root rot, leaf spot and fall) in sugar beet; Phomopsis spp. in sunflowers, vines (for example, P. vitícola: can and leaf spot) and soy (for example, stem rot: P. phaseoli, teleomorph: Diaporthe phaseolorum); Physoderma maydis (brown spots) in corn; Phytophthora spp. (wilting, root rot, leaf, fruit and stem) in several plants, such as paprika and cucurbits (for example, P. capsici), soybeans (for example, P. megasperma, syn. P. sojae), potatoes and tomatoes (for example, P. infestans: late blight) and broadleaf trees (for example, P. ramorum: sudden oak death); Plasmodiophora brassicae (root distortion (club root)) in cabbage, rapeseed, radish and other plants; Plasmopara spp., For example, P. vitícola (vine mildew) in vines and P. halstedii in sunflowers; Podosphaera spp. (powdery mildew) in rosaceous plants, hops, pomegranate and soft fruits, for example, P. leucotricha in apple; Polymyxa spp., For example, in cereals, such as barley and wheat (P. graminis) and sugar beet (P. betae) and, therefore, viral diseases transmitted; Pseudocercosporella herpotrichoides (ocular spot, teleomorph: Tapesia yailundae) in cereals, for example, wheat or barley; Pseudoperonospora (mildew) in several plants, for example, P. cubensis in cucurbits or P. humili in hops; Pseudopezicula tracheiphila (disease that causes red color or "rotbrenner", anamorph: Phialophora) on vines; Puccinia spp. (rusts) in several plants, for example, P. triticina (leaf rust or brown), P. striiformis (striped rust or yellow), P. hordei (dwarf rust), P. graminis (stem rust or black ) or recondite P. (brown or leaf rust) in cereals, such as, for example, wheat, barley or rye and asparagus (for example, P. asparagi); Pyrenophora (anamorph: Drechslera) tritici-repentis (brown spot) in wheat or P. teres (net spot) in barley; Pyricularia spp., For example, P. oryzae (teleomorph: Magnaporthe grísea, rice fungus) in rice and P. grísea in grass and cereals; Pythium spp. (fall) in grass, rice, corn, wheat, cotton, rapeseed, sunflower, soybeans, sugar beet, vegetables and several other plants (for example, P. ultimum or P. aphanidermatum); Ramularia spp., For example, R. collo-cygni (leaf spots Ramularia, physiological spots on leaves) on barley and R. beticola on sugar beet; Rhizoctonia spp. in cotton, rice, potato, turf, corn, rapeseed, potato, sugar beet, vegetables and several other plants, for example, R. solani (root rot and stem) in soybeans, R. solani (pod blight) in rice or R. cerealis (spring blight Rhizoctonia) in wheat or barley; Rhizopus stolonifer (black mold, slight rot) in strawberries, carrots, cabbages, vines and tomatoes; Rhynchosporium secalis (scald) in barley, rye and triticale; Sarocladium oryzae and S. attenuatum (pod rot) in rice; Sclerotinia spp. (stem rot or white mold) in vegetables and field crops, such as rapeseed, sunflower (for example, S. sclerotiorum) and soybean (for example, S. rolfsii or S. sclerotiorum); Septoria spp. in several plants, for example, S. glycines (brown spot) in soybeans, S. tritici (Septoria spot) in wheat and S. (syn. Stagonospora) nodorum (Stagonospora spot) in cereals; Uncinula (syn. Erysiphe) necator (powdery mildew, anamorph: Oidium tuckeri) on vines; Setospaeria spp. (leaf blight) in corn (for example, S. turcicum, syn. Helminthosporium turcicum) and turf; Sphacelotheca spp. (soot) in corn, (for example, S. reiliana: top soot), sorghum and sugarcane; Sphaerotheca fuliginea (powdery mildew) in cucurbits; Underground spongospora (powdery crust) in potatoes and, therefore, transmitted viral diseases; Stagonospora spp. in cereals, for example, S. nodorum (Stagonospora stain, teleomorph: Leptosphaeria [syn. Phaeosphaeria] nodorum) in wheat; Synchytrium endobioticum in potato (potato wart disease); Taphrina spp., For example, T. deformans (leaf rolling disease) in peach and T. pruni (cavities in plum) in plums; Thielaviopsis spp. (black root rot) in tobacco, pomo fruits, vegetables, soybean and cotton, for example, T. basteóla (syn. Chalara elegans); Tilletia spp. (common coal or smelly soot) in cereals, such as, for example, T. tritici (syn.T.caries, wheat charcoal) and T. controvert (dwarf charcoal) in wheat; Typhula incarnata (gray snow mold) in barley or wheat; Urocystis spp., For example, U. oceulta (stem soot) in rye; Uromyces spp. (rust) on vegetables, such as beans (for example, U. appendiculatus, syn.U. phaseoli) and sugar beet (for example, U. betae); Ustilago spp. (loose soot) in cereals (for example, U. nuda and U. avaenae), corn (for example, U. maydis: corn soot) and sugarcane; Venturia spp. (crust) in apples (for example, V. inaequalis) and pears; and Verticillium spp. (wilting) in various plants, such as fruits and ornamentals, vines, soft fruits, vegetables and field crops, for example, V. dahliae in strawberries, rapeseed, potatoes and tomatoes.
Another main problem of the present invention is the desire to obtain compositions that improve the health of a plant, a process that is commonly referred to as "plant health". The term plant health comprises several types of plant improvements that are not related to the control of pests and that do not include the reduction of negative consequences of harmful fungi. The term "plant health" indicates a condition of the plant and / or its products that is determined by various indicators, alone or in combination, such as yield (eg, higher biomass and / or higher content of valuable ingredients). ), vigor of the plant (eg, better growth of the plant and / or greener leaves ("more green effect"), quality (eg, better content or composition of certain ingredients) and tolerance to abiotic stress and / or biotic The indicators identified above for the health condition of a plant may be interdependent or may be the result of one another.
Therefore, an objective of the present invention was to provide a method, which solved the problems indicated above and, in particular, to reduce the dosage rate and / or promote the health of a plant.
Surprisingly, it has now been discovered that the use of carboxamide compounds, as defined above, in the cultivated plants shows a synergistic effect between the trait of the cultivated plant and the applied carboxamide.
In the present context, synergistic means that a) the use of a carboxamide compound, as defined above, in combination with a cultivated plant exceeds the addition effect that is expected in the harmful fungi to be controlled and, thus, extends the range of action of the carboxamide compound and of the active principle expressed by the cultivated plant, and / or b) said use results in a greater effect on the health of the plant in said cultivated plants compared to the health effects of the plant that are possible with the carboxamide compound, when applied to the non-cultivated plant; I c) the carboxamide compound induces "side effects" in the cultivated plant that increase the health of the plant, in comparison with the respective control plant, in addition to the primary mode of action, ie, the fungicidal activity; I d) the carboxamide compound induces additional "side effects" to the primary mode of action, i.e., the fungicidal activity in the control plant that is detrimental to the health of the plant as compared to a control plant that is not treated with said compound. In combination with the cultivated plant, these negative side effects are reduced, nullified or converted into greater health of the cultivated plant compared to a cultivated plant not treated with said compound.
Thus, the term "synergistic" means, in this context, synergistic fungicidal activity and / or the synergistic increase of the health of the plant.
In particular, it has been found that applying at least one compound of carboxamide, as defined above, to the cultivated plants generates an improved synergistic action against the harmful fungi compared to the control rates that are possible with the carboxamide compound, as defined above, in uncultivated plants and / or leads to a synergistic increase in the health of a plant when applied to a cultivated plant, parts of a plant, plant propagation material or its growth locus.
Therefore, the present invention relates to a method for controlling harmful fungi and / or increasing the health of a cultivated plant by treating a cultivated plant, parts of a plant, plant propagation material or its growth locus with a carboxamide compound selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen , fluopiram, sedaxane, isopyrazam, pentiopyrad, benodanil, carboxy, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxin, trifluzamide, preferably with a carboxamide compound selected from the group consisting of boscalid, N- (3 ', 4', 5 ' -trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopyrad.
The carboxamide compounds are known as fungicides (cf., for example, EP-A 545 099, EP-A 589 301, EP-A 737682, EP-A 824099, WO 99/09013, WO 03/010149, WO 03 / 070705, WO 03/074491, WO 2004/005242, WO 2004/035589, WO 2004/067515, WO 06/087343). For example, commercially available compounds can be found in The Pesticide Manual, 13th Edition, British Crop Protection Council (2003), among other publications.
The term "plant propagation material" indicates all generative parts of a plant, such as seeds and vegetative material of the plant, such as cuttings and tubers (e.g., potatoes), which can be used for the multiplication of the plant. This includes seeds, roots, fruits, tubers, bulbs, rhizomes, shoots, shoots and other parts of plants, including seedlings and young plants, which are going to be transplanted after germination or after they emerge from the soil. These young plants can also be protected before being transplanted with a total or partial treatment by immersion or spillage. Preferably, the term plant propagation material refers to seeds.
In a preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of a cultivated plant by treatment of plant propagation material, preferably seeds, with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H -pyrazol-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam, pentiopyrad, benodanil, carboxy, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluoro-phenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopyrad, most preferably with boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, penflufen, fluopyram, sedaxane and pentyopripe.
The present invention also comprises plant propagation material, preferably seeds, of a cultivated plant treated with a carboxamide as defined above, preferably boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3- difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam, pentiopyrad, benodanil, carboxy, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxin, trifluzamide, preferably with a selected carboxamide compound from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxan , isopyrazam and pentiopyrad, most preferably with boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, penflufen, fluopyramide , sedaxano and pentiopirad.
In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of a cultivated plant by treating the cultivated plant, part (s) of said plant or its growth rate with a compound, of carboxamide selected, preferably, from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam, pentiopyrad, benodanil, carboxy, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxin, trifluzamide, more preferably with a carboxamide compound selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopyrad, most preferably boscalid, N- (3 ' , 4 ', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, fluopyram, isoprotaza m and pentiopirad.
In another embodiment, the present invention relates to a composition comprising a pesticide and a cultivated plant or its parts or cells, wherein the pesticide is a carboxamide compound, preferably selected from the group consisting of boscalid, N- ( 3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam, pentiopyrad, benodanil, carboxy, fenfuram , flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1 -methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopyrad, most preferably boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3 -difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, fluopyram, isopyrazam and pentiopyrad. Said compositions may include other pesticides and other carboxamides or several of the carboxamides of the group described in the previous sentence. Said compositions may include substances used for the protection of plants and, in particular, in the formulation of products for the protection of plants. The composition of the invention may comprise live plant material or plant material that can not be propagated, or both. The composition may contain plant material from more than one plant. In a preferred embodiment, the ratio between plant material of at least one cultivated plant and pesticide, on a weight-by-weight basis, is greater than 10 to 1, preferably greater than 100 to 1, or more preferably greater than 1000. to 1, even more preferably greater than 10 000 to 1. In some cases, a proportion greater than 100,000 or one million is preferred.
In one embodiment, "agricultural composition" means that said composition complies with the laws that regulate the content of fungicides, plant nutrients, herbicides, etc. Preferably, said composition does not cause damage to the protected plants and / or the animals (including humans) that feed on them.
In another embodiment, the present invention relates to a method for the production of an agricultural product comprising the application of a pesticide to plants grown with at least one modification, parts of said plants, plant propagation materials or their locus of growth, and the production of the agricultural product from the parts of said plants or plant propagation materials, wherein the pesticide is a carboxamide compound, preferably selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam, pentiopyrad, benodanil, carboxy, fenfuram, flutolanil, furametpyr, mepronil , oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H- pyrazole-4-carboxamide, bixafen, penflu fen, fluopyram, sedaxane, isopyrazam and pentiopyrad, most preferably boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4- carboxamide, bixafen, fluopiram, isopyrazam and pentiopyrad.
In one embodiment of the invention, the term "agricultural product" is defined as the result of the cultivation of the soil, for example, grain, forage, fruit, fiber, flower, pollen, leaves, tuber, root, beet or seed.
In one embodiment of the invention, the term "agricultural product" is defined in accordance with the definition of "agricultural products" by the USDA (United States Department of Agriculture). Preferably, "agricultural product" means "products for food and fiber", which encompass a wide range of goods from unprocessed raw materials in bulk such as soy, corn for food, wheat, rice and unprocessed cotton to beverages and high-value, highly processed foods such as sausages, bakery products, ice cream, beer and wine, and condiments sold in retail stores and restaurants. In one embodiment, "agricultural product" are products that are found in Chapters 4, 6-15, 17-21, 23-24, Chapter 33 and Chapter 52 of the U.S. Harmonized Tariff Schedule (December 1993, which took place as a result of the Uruguay Round Agreements) based on the Harmonized Commodity Coding and Classification System (Harmonized System), established by the Organization. World Customs). The agricultural products according to the invention within these chapters are preferably within the following categories: grains, products for animal feed and grain products (such as bread and pasta); oilseeds and oilseed products (such as soybean oil and olive oil); horticultural products, including all fruits, vegetables, fresh and processed nut trees, as well as greenhouse products, unprocessed tobacco; and tropical products such as sugar, cocoa and coffee. In one embodiment, "agricultural product" is a product selected from the group of products found in the U.S. Harmonized Tariff Schedule under the items: 0409, 0601 to 0604, 0701 to 0714, 0801 to 0814, 0901 to 0910, 1001 to 1008, 1 101 to 1 109, 1201 to 1214, 1301 a 1302, 14 01 to 1404, 1507 to 1522, 1701 to 1704, 1801 to 1806, 1901 to 1905, 2001 to 2009, 2101 to 2106, 2302 to 2309, 2401 to 2403, 3301, 5201 to 5203.
The term "cultivated plant (s)" refers to "modified plant (s)" and "transgenic plant (s)".
In one embodiment of the invention, the term "cultivated plants" refers to "modified plants".
In one embodiment of the invention, the term "cultivated plants" refers to "transgenic plants". "Modified plants" are those that have been modified by conventional breeding techniques. The term "modification" means, in relation to the modified plants, a change in the genome, epigenome, transcriptome or proteome of the modified plant, in comparison with the control plant, wild type, mother or progenitor by which the modification confers a trait (or more than one trait) or confers the increase of a trait (or more than one trait) as listed below.
The modification may result in the modified plant being different, for example, a new plant variety with respect to the parent plant.
"Transgenic plants" are those whose genetic material has been modified by the use of recombinant DNA techniques that, under natural circumstances, can not be obtained by cross-breeding, mutations or natural recombination, whereby the modification confers a trait (or more of a trait) or confers the increase of a trait (or more than one trait) as listed below as compared to the wild type plant.
In one embodiment, one or more genes are integrated into the genetic material of a genetically modified plant in order to improve certain properties of the plant, preferably to augment a trait of those listed below as compared to the wild-type plant. Such genetic modifications also include, but are not limited to, post-translationally directed modification of protein (s) or post-transcriptional modifications of oligopeptides or polypeptides, for example, by glycosylation or additions of polymers such as prenylated portions. , acetylated, phosphorylated or farnesylated or PEG portions.
In one embodiment, the term "modification", when referring to a transgenic plant or its parts, means that it changes the activity, expression level or amount of a gene product or metabolite content, for example, which increases or it decreases, in a specific volume in relation to a corresponding volume of a plant or control plant cell, reference or wild type, including the creation of the activity or expression.
In one embodiment, the activity of a polypeptide is increased or generated by expression or overexpression of the gene encoding said polypeptide that confers a trait or confers the increase of a trait as listed below as compared to the control plant . The term "expression" or "gene expression" means the transcription of a specific gene or specific genes or specific genetic construct. In particular, the term "expression" or "gene expression" means the transcription of a gene or genes or genetic construct into a structural RNA (rRNA, tRNA), regulatory RNA (e.g., miRNA, RNAi, aRNA) or mRNA with or without the subsequent translation of the latter into a protein. In another embodiment, the term "expression" or "gene expression" means, in particular, the transcription of a gene or genes or genetic construct in structural RNA (rRNA, tRNA) or mRNA with or without the subsequent translation of the latter in a protein. In yet another embodiment, it means the transcription of a gene or genes or genetic construct in mRNA.
The process includes the transcription of DNA and the processing of the resulting mRNA product. The term "greater expression" or "overexpression," as used herein, means any form of expression that is in addition to the original expression level of the wild type.
In one embodiment, the term "expression of a polypeptide" means the level of said protein or polypeptide, preferably in an active form, in a cell or organism.
In one embodiment, the activity of a polypeptide is decreased by decreasing the expression of the gene encoding said polypeptide that confers a trait or confers the increase of a trait as listed below, as compared to the control plant. The reference herein made to "minor expression" or "substantial reduction or elimination" of the expression means a decrease in the expression of an endogenous gene and / or in the levels of polypeptides and / or in the activity of polypeptides in relationship with control plants. It comprises reducing, repressing, decreasing or further eliminating the expression of a product of a nucleic acid molecule.
The terms "reduction", "repression", "decrease" or "elimination" refer to a corresponding change of a property in an organism, a part of an organism such as a tissue, seed, root, tuber, fruit, leaf, flower, etc. or in a cell. "Change of a property" means that the activity, level of expression or quantity of a gene product or metabolite content changes in a specific volume or in a specific amount of protein in relation to the corresponding volume or amount of protein. a control, reference or wild type. Preferably, in some cases the overall activity in the volume is reduced, reduced or eliminated if the reduction, decrease or elimination is related to the reduction, decrease or elimination of an activity of a gene product, regardless of whether the amount of gene product or the specific activity of the gene product or both is reduced, decreased or eliminated or if the amount, efficiency of translation or stability of the nucleic acid sequence or of the gene encoding the gene product is reduced, decreased or eliminated.
The terms "reduction", "repression", "decrease" or "elimination" include the change of said property only in parts of the subject of the present invention, for example, the modification can be found in a compartment of a cell, such as a organelle, or in a part of a plant, such as tissue, seed, root, leaf, tuber, fruit, flower, etc. but it can not be detected if the whole subject is evaluated, that is, the plant or whole cell. Preferably, the "reduction", "repression", "decrease" or "elimination" is found at the cell level, thus, the term "reduction, decrease or elimination of an activity" or "reduction, decrease or elimination of a metabolite content "refers to the reduction, decrease or elimination at the cell level compared to the wild-type cell. Also, the terms "reduction", "repression", "decrease" or "elimination" include the change of said property only during different phases of the growth of the organism used in the process of the invention, for example, reduction, repression, decrease or elimination occur only during the growth of the seed or during flowering. Also, the terms include a transient reduction, decrease or deletion, for example, because the method used, for example, the antisense molecule, RNAi, snRNA, dsRNA, siRNA, miRNA, ta-siRNA, cosuppression or ribozyme is not Stably integrated into the genome of the organism or the reduction, decrease, repression or elimination is under the control of a regulatory or inducible element, for example, a promoter inducible by chemical products or otherwise inducible and, therefore, it only has a transitory effect.
The methods to achieve said reduction, reduction or elimination in a Expression product are known in the art, for example, in international patent application WO 2008/034648, in particular in paragraphs [0020.1.1.1], [0040.1.1.1], [0040.2.1.1] and [0041.1.1.1]. ] It is known to reduce, repress, reduce or eliminate an expression product of a nucleic acid molecule in modified plants. The examples are canola, that is, double zero oilseed rape with reduced amounts of erucic acid and sinapines.
Such a decrease can also be achieved, for example, by the use of recombinant DNA technology, such as siRNA approaches or antisense or regulatory RNA (e.g., miRNA, RNAi, aRNA). In particular, RNAi, snRNA, dsRNA, siRNA, miRNA, ta-siRNA, cosuppression, ribozyme, or antisense nucleic acid molecule, a nucleic acid molecule that confers the expression of a dominant-negative mutant of a construct can be used. of nucleic acid or a protein capable of recombining and silencing, inactivating, repressing or reducing the activity of an endogenous gene, to decrease the activity of a polypeptide in a transgenic plant or its parts or its plant cell used in an embodiment of the methods of the invention. Examples of transgenic plants with a reduced, repressed, diminished or eliminated expression product of a nucleic acid molecule are Carica papaya (papaya plants) named event X17-2 of the University of Florida, Prunus domestica (plum) with event name C5 of the United States Department of Agriculture - Agricultural Research Service, or those listed in rows T9-48 and T9-49 of table 9 below. Plants with greater resistance to nematodes are also known, for example, by reducing, repressing, reducing or eliminating an expression product of a nucleic acid molecule, for example, from PCT publication WO 2008/095886.
The reduction or substantial elimination is, in order of increasing preference, at least 10%, 20%, 30%, 40% or 50%, 60%, 70%, 80%, 85%, 90% or 95%, 96% , 97%, 98%, 99% or more reduction compared to the control plants. The reference herein to an "endogenous" gene not only refers to the gene in question as it is found in a plant in its natural form (ie, without human intervention), but also refers to that same gene ( or to a gene / nucleic acid substantially homologous) in isolated form that is (re) introduced later in a plant (a transgene). For example, a transgenic plant containing said transgene can present a substantial reduction of the expression of the transgene and / or a substantial reduction of the expression of the endogenous gene.
The terms "control" or "reference" may be used interchangeably and may be a cell or a part of a plant, such as an organelle such as a chloroplast or a tissue, in particular a plant that was not modified or treated in accordance with the process described herein according to the invention. Accordingly, the plant used as control or reference corresponds to the plant as much as possible and is as identical to the object of the invention as possible. In this way, the control or the reference is treated identically or as identical as possible, that is to say that only conditions or properties that do not influence the quality of the evaluated property and that are different from the treatment of the present invention.
It is possible that the control or reference plants are wild type plants. However, "control" or "reference" may refer to plants that carry at least one genetic modification, when the plants used in the process of the present invention carry at least one genetic modification more than said control or reference plants. . In one embodiment, the control or reference plants may be transgenic but differ from the transgenic plants employed in the process of the present invention only by said genetic modification contained in the transgenic plants employed in the process of the present invention.
The term "wild type" or "wild-type plants" refers to a plant without said genetic modification. These terms may refer to a cell or a part of a plant, such as an organelle, such as a chloroplast or a tissue, in particular a plant that lacks such genetic modification but is otherwise as identical as possible to plants with at least one genetic modification employed in the present invention. In a particular embodiment, the "wild type" plant is not transgenic.
Preferably, the wild type is treated identically in accordance with the process described herein according to the invention. The skilled artisan will recognize whether the wild-type plants will not require certain pretreatments to the process of the present invention, for example, non-transgenic wild-type plants will not need selection of transgenic plants, for example, by treatment with an agent of selection, such as a herbicide.
The control plant can also be a nulicigota of the plant to be evaluated. The term "nulicigotas" refers to a plant that has been subjected to the same production process as a transgenic plant and, however, has not acquired the same genetic modification as the corresponding transgenic plant. If the starting material of said production process is transgenic, then the nulli-cysts are also transgenic but lack the additional genetic modification introduced by the production process. In the process of the present invention, the purpose of the wild type and the nulicigota is the same as for the control or reference or its parts. All of these serve as controls in any comparison to provide evidence of the advantageous effect of the present invention.
Preferably, any comparison is carried out under analogous conditions.
The term "analogous conditions" means that all conditions such as, for example, cultivation or development conditions, soil, nutrients, water content of the soil, temperature, humidity or air or surrounding soil, test conditions (such as composition of the buffer, temperature, substrates, strain of pathogens, concentrations and the like) remain identical between the experiments to be compared. The artisan will recognize whether wild-type, control or reference plants will not require certain pre-treatments of the process of the present invention, for example, non-transgenic wild-type plants will not need selection of transgenic plants, for example, by treatment with a herbicide.
In case the conditions are not analogous, the results can be standardized or standardized on the basis of control.
The "reference", the "control" or the "wild type" preferably is a plant that has not been modified or treated in accordance with the process of the invention described herein and is, in any other property, as similar as it may be. possible to a plant employed in the process of the present invention. The reference, control or wild type is, in its genome, transcriptome, proteome or metabolome, as similar as possible to a plant employed in the process of the present invention. Preferably, the term "reference", "control" or "wild type" plant refers to a plant, which is almost genetically identical to the organelle, cell, tissue or organism, in particular plant, of the present invention or a part of this, preferably 90% or more, for example, 95%, more preferably 98%, even more preferably 99.00%, in particular 99.10%, 99.30%, 99.50%, 99.70% %, 99.90%, 99.99%, 99.999% or more. Most preferably, the "reference", the "control" or the "wild type" is a plant, which is genetically identical to the plant, cell, tissue or organelle used according to the process of the invention, except that the nucleic acid molecules responsible for or conferring activity or the gene product encoded by them have been modified, manipulated, exchanged or introduced into the organelle, cell, tissue, plant employed in the process of the present invention.
Preferably, the reference and the object of the invention are compared after standardization and normalization, for example, with the total amount of RNA, DNA or protein or activity or expression of the reference genes, such as housekeeping genes, such as ubiquitin, actin or ribosomal proteins.
The genetic modification that is carried out in the organelle, cell, tissue, in particular in the plant used in the process of the present invention, in one embodiment, is stable, for example, due to a stable transgenic expression or to a stable mutation in the corresponding endogenous gene or to a modulation of the expression or behavior of a gene, or transient, for example, due to a transient transformation or temporary addition of a modulator, such as an agonist or antagonist, or inducible, for example , after transformation with an inducible construct carrying a nucleic acid molecule under the control of an inducible promoter and by the addition of the inducer, for example, tetracycline.
Preferred plants according to the invention, from which the "modified plants" and / or "transgenic plants" are selected, are selected from the group consisting of cereals, such as corn, wheat, sorghum barley, rice, rye, millet. , triticale, oats, pseudocereals (such as buckwheat and quinoa), alfalfa, apple, banana, beet, broccoli, Brussels sprouts, cabbage, cañola (rapeseed), carrot, cauliflower, cherries, chickpea, Chinese cabbage, Chinese mustard, cabbage, cotton, cranberry, creeping bentgrass, cucumber, eggplant, flax, grape, grapefruit, cabbage, kiwi, kohlrabi, melon, mizuna, mustard, papaya, peanut, pears, pepper, kaki, pigeon pea, pineapple, plum, plum, potato, raspberry, Swedish turnip, soybean, pumpkin, strawberries, sugar beet, sugar cane, sunflower, sweet corn, tobacco, tomato, turnip, walnut, watermelon and winter squash; preferably the plants are selected from the group consisting of alfalfa, barley, cañola (rapeseed), cotton, corn, papaya, potato, rice, sorghum, soybeans, squash, sugar beet, sugar cane, tomato and cereals (such as wheat, barley, rye and oats), most preferably the plant is selected from the group consisting of soy, corn, rice, cotton, oilseed rape, tomato, potato and cereals such as wheat, barley, rye and oats.
In another embodiment of the invention, the cultivated plant is a gymnosperm plant, especially spruce, pine or spruce.
In one embodiment, the cultivated plant is selected from the families Aceraceae, Anacardiaceae, Apiaceae, Asteraceae, Brassicaceae, Cactaceae, Cucurbitaceae, Euphor-biaceae, Fabaceae, Malvaceae, Nymphaeaceae, Papaveraceae, Rosaceae, Salicaceae, Solanaceae, Arecaceae, Bromeliaceae, Cyperaceae, Iridaceae, Liliaceae, Orchidaceae, Gentianaceae, Labiaceae, Magnoliaceae, Ranunculaceae, Carifolaceae, Rubiaceae, Scrophulariaceae, Caryophyllaceae, Ericaceae, Polygonaceae, Violaceae, Juncaceae or Poaceae and preferably from a plant selected from the group of the families Apiaceae, As-teraceae, Brassicaceae, Cucurbitaceae, Fabaceae, Papaveraceae, Rosaceae, Solanaceae, Liliaceae or Poaceae.
The crop plants and, in particular, plants selected from the families and genera mentioned above are preferred, for example, the species Anacardium occidentale, Calendula officinalis, Carthamus tinctorius, Cichorium intybus, Cynara scolymus, Helianthus annus, Tagetes lucida are preferred. , Tagetes erecta, Tagetes tenuifolia; Daucus carota; Corylus avellana, Corylus colurna, Borago officinalis; Brassica napus, Brassica rapa ssp., Sinapis arvensis Brassica júncea, Brassica júncea var. júncea, Brassica júncea var. crispifolia, Brassica júncea var. foliosa, Brassica nigra, Brassica sinapioides, Melanosinapis communis, Brassica oleracea, Arabidopsis thaliana, Anana comosus, Ananas ananas, Bromelia comosa, Carica papaya, Cannabis sative, Ipomoea batatus, Ipomoea pandurata, Convolvulus batatas, Convolvulus tiliaceus, Ipomoea fas-tigiata, Ipomoea tiliacea, Ipomoea triloba, Convolvulus panduratus, Beta vulgaris, Beta vulgaris var. altissima, Beta vulgaris var. vulgaris, maritime Beta, Beta vulgaris var. perennis, Beta vulgaris var. conditive, Beta vulgaris var. esculenta, Cucurbita maximus, Cucurbita mixta, Cucurbita pepo, Cucurbita moschata, Olea europaea, Manihot utilissima, Janipha manihot, Jatropha manihot., Manihot aipil, Manihot dulcis, Manihot manihot, Manihot melanobasis, Manihot esculenta, Ricinus communis, Pisum sativum, Pisum arvense , Pisum humile, Medicago sativa, Medicago falcata, Various medicago, Glycine max Dolichos soybean, Glycine gracilis, Glycine hispida, Phaseolus max, Soybean hispida, Soy max, Cocos nucifera, Pelargonium grossularioides, Oleum cocoas, Laurus nobilis, Persea americana, Arachis hypogaea , Linum usitatissimum, Linum humile, Linum austriacum, Linum bienne, Linum angustifolium, Linum catharticum, Linum flavum, Linum grandiflorum, Adenolinum grandiflo-rum, Linum lewisii, Linum narbonense, Linum perennial, Linum perennial var. lewisii, Linum pratense, Linum trigynum, Punic granatum, Gossypium hirsutum, Gossypium arboreum, Gossypium barbadense, Gossypium herbaceum, Gossypium thurberi, Musa nana, Musa acuminata, Musa paradisiaca, Musa spp., Elaeis guineensis, Papaver oriéntale, Papaver rhoeas, Papaver dubium , Sesamum indicum, Piper aduncum, Piper amalago, Piper angus-tifolium, Piper auritum, Piper betel, Piper cubeba, Piper longum, Piper nigrum, Piper ret-rofractum, Artanthe adunca, Artanthe elongata, Peperomia elongata, Piper elongatum, Steffensia elongata, Hordeum vulgare, Hordeum jubatum, Hordeum murinum, Hordeum secalinum, Hordeum distichon Hordeum aegiceras, Hordeum hexastichon, Hordeum hexa-stichum, Hordeum irregulare, Hordeum sativum, Hordeum secalinum, Avena sativa, Avena fatua, Avena byzantina, Avena fatua var. sativa, Avena hybrida, Sorghum bicolor, Sorghum halepense, Sorghum saccharatum, Sorghum vulgare, Andropogon drummondii, Holcus bi-color, Holcus sorghum, Sorghum aethiopicum, Sorghum arundinaceum, Sorghum caf-frorum, Sorghum cernuum, Sorghum dochna, Sorghum drummondii, Sorghum durra , - Sor-ghum guiñéense, Sorghum lanceolatum, Sorghum nervosum, Sorghum saccharatum, Sorghum subglabrescens, Sorghum verticilliflorum, Sorghum vulgare, Holcus halepensis, Sorghum miliaceum millet, Panicum militaceum, Zea mays, Triticum aestivum, Triticum durum, Triticum turgidum, Triticum hybernum, Triticum macha, Triticum sativum or Triticum vulgare, Cofea spp., Coffea arabica, Coffea canephora, Coffea liberica, Capsicum annuum, Capsi-cum annuum var. glabriusculum, Capsicum frutescens, Capsicum annuum, Nicotiana tabacum, Solanum tuberosum, Solanum melongena, Lycopersicon esculentum, Lycopersicon lycopersicum, Lycopersicon pyriforme, Solanum integrifolium, Solanum lycopersicum Theobroma cacao and Camellia sinensis.
Anacardiaceae such as the genus Pistacia, Mangifera, Anacardium, for example, the species Pistacia vera [pistachio, Pistazie], Mangifer indica [mango] or Anacardium occi-dentale [cashew nut], Asteraceae such as the genus Calendula, Carthamus, Centaurea , Cichorium, Cynara, Helianthus, Lactuca, Locusta, Tagetes, Valeriana for example, the species Calendula officinalis [Calendula], Carthamus tinctorius [safflower], Centaurea cyanus [flower of corn], Cichorium intybus [blue daisy], Cynara scolymus [Alcaucil ], Helianthus annus [sunflower], Lactuca sativa, Lactuca crispa, Lactuca esculenta, Lactuca scariola L. ssp. sativa, Lactuca scariola L. var. whole, Lactuca scariola L. var. integrifolia, Lactuca sativa subsp. Roman, Locusta communis, Valeriana locusta [lettuce], Tagetes lucida, Tagetes erecta or Tagetes tenuifolia [Calendula]; Apiaceae such as the genus Daucus for example, the species Daucus carota [carrot]; Betulaceae such as the genus Coryius for example, the species Coryius avellana or Coryius colurna [hazelnut]; Boraginaceae such as the genus Borago for example, the species Borago officinalis [borraja]; Brassicaceae such as the genus Brassica, Melanosinapis, Sinapis, Arabadopsis for example, the species Brassica napus, Brassica rapa ssp. [canola, rapeseed, turnip rape], Sinapis arvensis Brassica júncea, Brassica júncea var. júncea, Brassica júncea var. crispifolia, Brassica júncea var. foliosa, Brassica nigra, Brassica synapioides, Melanosinapis communis [mustard], Brassica olerácea [fodder beet] or Arabidopsis thaliana; Bromeliaceae such as the genus Anana, Bromelia for example, the species Anana comosus, Ananas ananas or Bromelia comosa [ananá]; Caricaceae such as the genus Carica for example, the species Carica papaya [papaya]; Cannabaceae such as the genus Cannabis for example, the species Cannabis sative [hemp], Convolvulaceae such as the genus Ipomea, Convolvulus for example, the species Ipomoea batatus, Ipomoea pandurata, Convolvulus batatas, Convolvulus tiliaceus, Ipomoea fastigiata, Ipomoea tiliacea, Ipomoea triloba or Convolvulus panduratus [Batata, Man of the Earth, wild potato], Chenopodiaceae such as the genus Beta, that is, the species Sera vulgaris, Beta vulgaris var. altissima, Beta vulgaris var. Vulgaris, maritime Beta, Beta vulgaris var. perennis, Beta vulgaris var. conditive or Sera vulgaris var. esculenta [sugar beet]; Cucurbitaceae such as the genus Cucubita for example, the species Cucurbita maximus, Cucurbita mixta, Cucurbita pepo or Cucurbita moschata [squash, pumpkin]; Elaeagnaceae such as the genus Elaeagnus for example, the species Olea europaea [oliva]; Ericaceae such as the genus Kalmia for example, the species Kalmia latifolia, Kalmia angustifolia, Kalmia microphylla, Kalmia polifolia, Kalmia occidentalis, Cistus chamaerhodendros or Kalmia lucida [American laurel, broadleaf laurel, calico shrub, spoon wood, laurel sheep, laurel alpine, swamp laurel, western marsh laurel, laurel of the swamp]; Euphorbiaceae such as the genus Manihot, Janipha, Jatropha, Ricinus for example, the species Manihot utilissima, Janipha manihot, Jatropha manihot, Manihot aipil, Manihot dulcis, Manihot manihot, Manihot melanobasis, Manihot esculenta [cassava, maranta, tapioca, casava] or Ricinus communis [castor beans, castor oil bush, castor oil plant, Palma Christi, Wonder Tree]; Fabaceae such as the genus Pisum, Albizia, Cathormion, Feuillea, Inga, Pithecolobium, Acacia, Mimosa, Medicajo, Glycine, Dolichos, Phaseolus, Soya for example, the species Pisum sativum, Pisum arvense, Pisum humile [pea], Albizia berteriana, Albizia julibrissin, Albizia lebbeck, Acacia berteriana, Acacia littoralis, Albizia berteriana, Albizzia berteriana, Cathormion berteriana, Feuillea berteriana, Inga fragrans, Pithecellobium berterianum, Pithecellobium fragrans, Pithecolobium berterianum , Pseudalbizzia berteriana, Acacia julibrissin, Acacia nemu, Albizia nemu, Feuilleea julibrissin, Mimosa julibrissin, Mimosa speciosa, Sericanrda julibrissin, Acacia lebbeck, Acacia macrophylla, Albizia lebbek, Feuilleea lebbeck, Mimosa lebbeck, Mimosa speciosa [bastard logwood, albizia, Nogal East Indian], Medicago sativa, Medicago falcata, Medicago varies [alfalfa] Glycine max Dolichos soybean, Glycine gracilis, Glycine hispida, Phaseolus max, Soybean hispida or Soy max [soybean]; Geraniaceae such as the genus Pelargonium, Cocos, Oleum for example, the species Cocos nucífera, Pelargonium grossularioides or Oleum cocois [coconut]; Gramineae such as the genus Saccharum for example, the species Saccharum officinarum; Juglandaceae such as the genus Juglans, Wallia for example, the species Juglans regia, Juglans ailanthifolia, Juglans sieboldiana, Juglans cinerea, Wallia cinerea, Juglans bixbyi, Juglans californica, Juglans hindsii, Juglans intermedia, Juglans jamaicensis, Juglans major, Juglans microcarpa, Juglans nigra or Wallia nigra [walnut, black walnut, common walnut, Persian walnut, white walnut, butternut walnut, black walnut]; Lauraceae such as the genus Persea, Laurus for example, the laurel species Laurus nobilis [laurel, laurel, laurel bay, sweet laurel], Persea americana, Persea gratissima or Persea persea [avocado]; Leguminosae such as the genus Arachis for example, the species Arachis hypogaea [peanut]; Linaceae such as the genus Linum, Adenolinum for example, the species Linum usitatissimum, Linum humile, Linum austriacum, Linum bienne, Linum angustifolium, Linum catharticum, Linum flavum, Linum grandiflorum, Adeno-linum grandiflorum, Linum lewisii, Linum narbonense, Linum perennial , Linum perennial var. lewisii, Linum pratense or Linum trigynum [flax, flaxseed]; Lythrarieae such as the Punic genus for example, Punic granatum [granada]; Malvaceae such as the genus Gossypium for example, the species Gossypium hirsutum, Gossypium arboreum, Gossypium barbadense, Gossypium herbaceum or Gossypium thurberi [cotton]; Musaceae such as the genus Musa for example, the Musa nana species, Musa acuminata, Musa paradisiaca, Musa spp. [banana]; Onagraceae such as the genus Camissonia, Oenothera for example, the species Oenothera biennis or Camissonia brevipes [primula, evening primrose]; Palmae such as the genus Elacis for example, the species Elaeis guineensis [oil palm]; Papaveraceae such as the genus Papaver by example, Papaver species orient, Papaver rhoeas, Papaver dubium [poppy, oriental poppy, wild poppy, field poppy, shirley poppy, field poppy, long-headed poppy, long-pod poppy]; Pedaliaceae such as the genus Sesamum for example, the species Sesamum indicum [sesame]; Piperaceae such as the genus Piper, Artanthe, Peperomia, Steffensia for example, the species Piper aduncum, Piper amalago, Piper angustifolium, Piper auritum, Piper betel, Piper cubeba, Piper longum, Piper nigrum, Piper retrofractum, Artanthe adunca, Artanthe elongata, Peperomia elongata, Piper elongatum, Steffensia elongata. [Cayenne pepper, wild pepper]; Poaceae such as the genus Hordeum, Sécale, Oats, Sorghum, Andropogon, Holcus, Panicum, Oryza, Zea, Triticum for example, the species Hordeum vulgare, Hordeum jubatum, Hordeum murinum, Hordeum secalinum, Hordeum distichon Hordeum aegiceras, Hordeum hexastichon., Hordeum hexastichum, Hordeum irregulare, Hordeum sativum, Hordeum secalinum [barley, pearl barley, foxtail barley, wall barley, barley of dunes], Sécale cereale [rye], Avena sativa, Avena fatua, Avena byzantina, Avena fatua var. sativa, Avena hybrida [oats], Sorghum bicolor, Sorghum halepense, Sorghum saccharatum, Sorghum vulgare, Andropogon drummondii, Holcus bicolor, Holcus sorghum, Sorghum aethiopicum, Sorghum arundinaceum, Sorghum caffrorum, Sorghum cernuum, Sorghum dochna, Sorghum drummondii, Sorghum dune, Sorghum guiñéense, Sorghum lanceola-tum, Sorghum nervosum, Sorghum saccharatum, Sorghum subglabrescens, Sorghum ver-ticilliflorum, Sorghum vulgare, Holcus halepensis, Sorghum miliaceum millet, Panicum militaceum [sorghum, millet], Oryza sativa, Oryza latifolia [rice], Zea mays [maize] Triticum aestivum, Triticum durum, Triticum turgidum, Triticum hybernum, Triticum macha, Triticum sativum or Triticum vulgare [wheat, wheat for bread, common wheat], Proteaceae such as the genus Macadamia for example, the species Macadamia intergrifolia [macadamia ]; Rubiaceae such as Coffea for example, the species Cofea spp., Coffea arabica, Coffea canephora or Coffea iberica [coffee]; Scrophulariaceae such as the genus Verbascum for example, the species Verbascum blattaria, Verbascum chaixii, Verbascum densiflorum, Verbascum lagurus, Verbascum longifolium, Verbascum lychnitis, Verbascum nigrum, Verbascum olympicum, Verbascum phlomoides, Verbascum phoenicum, Verbascum pulverulentum or Verbascum thapsus [verbasco, verbasco of white moth, verbasco of needle-shaped leaves, verbasco densely flowered, silver verbasco, long-leaved verbasco, verbasco blanco, verbasco oscuro, verbasco greek, verbasco naranja, verbasco purpura, verbasco hoary, mullein]; Solanaceae such as the genus Capsicum, Nicotiana, Solanum, Lycopersicon for example, the species Capsicum annuum, Capsicum annuum var. glabriusculum, Capsicum frutescens [pepper], Capsicum annuum [paprika], Nicotiana tabacum, Nicotiana alata, Nicotiana attenuata, Nicotiana glauca, Nicotiana langsdorffii, Nicotiana obtusifolia, Nicotiana quadrivalvis, Nicotiana repanda, Nicotiana rustica, Nicotiana sylvestris [tobacco], Solanum tuberosum [ papa], Solanum melongena [aubergine], Lycopersicon esculentum, Lycopersicon lycopersicum., Lycopersicon pyriforme, Solanum in-tegrifolium or Solanum lycopersicum [tomato]; Sterculiaceae such as the genus Theobroma for example, the species Theobroma cacao [cacao]; Theaceae such as the genus Camellia for example, the species Camellia sinensis [tea].
In one embodiment, the cultivated plant is selected from the Viridiplantae superfamily, in particular from monocotledonous and dicotyledonous plants, including fodder or legumes for fodder, ornamental plants, food crops, trees or shrubs selected from the list comprising .Acer spp. ., Actinidia spp., Abelmoschus spp., Agave sisalana, Agropyron spp., Agrostis stolonifera, Allium spp., Amaranthus spp., Ammophila arenaria, Annona spp., Apium graveolens, Arachis spp, Artocarpus spp., Asparagus officinalis, Avena spp., Averrhoa carambola, Bambusa sp., Benincasa hispida, Bertholletia excelsea, Beta vulgaris, Brassica spp. Cadaba farinosa, Canna indica, Capsicum spp., Carex elata, Carissa macrocarpa, Carya spp., Castanea spp., Ceiba pentandra, Cichorium endive, Cinnamomum spp., Citrullus lanatus, Citrus spp., Cocos spp., Coffea spp., Colocasia esculenta, Cola spp., Corchorus sp., Coriandrum sativum, Crataegus spp., Crocus sativus, Cucurbit spp., Cucumis spp., Cynara spp., Daucus carota, Desmodium spp., Dimocarpus longan, Dioscorea spp., Diospyros spp., Echinochloa spp., Elaeis (eg, Elaeis oleifera), Eleusine coracana, Erapgrostis tef, Erianthus sp., Eriobotrya japonica, Eucalyptus sp., Eugenia uniflora, Fagopyrum spp., Fagus spp., Festuca arundinacea, Ficus carica, Fortunella spp. , Fragaria spp., Ginkgo biloba, Glycine spp. (for example, Glycine max, Soybean hispida or Soja max), Hemerocallis fulva, Hibiscus spp., Hordeum spp., Lathyrus spp., Lens culinaris, Litchi chinensis, Lotus spp., Luffa acutangula, Lupinus spp., Luzula sylvatica, Lycopersicon spp. Macrotyloma spp., Malus spp., Malpighia emarginata, Mammea americana, Manilkara zapota, Medicago sativa, Melilotus spp., Mentha spp., Miscanthus sinensis, Momordica spp., Morus nigra, Musa spp., Nicotiana spp., Olea spp., Opuntia spp., Ornithopus spp., Oryza spp., Panicum virgatum, Passiflora edulis, Pastinaca sativa, Pennisetum sp., Persea spp., Petroselinum críspum, Phalaris arundinacea, Phaseolus spp., Phleum pratense, Phoenix spp., Phragmites australis, Physalis spp. ., Pinus spp., Pisum spp., Poa spp., Populus spp., Prosopis spp., Prunus spp., Psidium spp., Pyrus communis, Quercus spp., Raphanus sativus, Rheum rhabarbarum, Ribes spp., Rt bt / spp., Saccharum spp., Sa // x sp., Sambucus spp., Sécale cereale, Sesamum spp., Sinapis sp., Solanum spp., Spinacia spp., Syzygium spp., Tagetes spp., Tamarindus indica, Theobroma cocoa, Trifolium spp., Tripsacum dactyloides, Triticosecale rimpaui, Triticum spp. (for example, Triticum monococcum), Tropaeolum minus, Tropaeolum majus, Vaccinium spp., V / 'c / a spp., Wgna spp., Wo / a odorata, Vitis spp., Zizania palustris, Ziziphus spp., among others.
Cultivated plants are plants that comprise at least one trait. The term "trait" refers to a property that is present in the plant either by genetic engineering or by conventional breeding techniques. Each feature must be evaluated in relation to its respective control. Examples of features are: • tolerance to herbicides, · Resistance to insects through the expression of bacterial toxins, • fungal resistance or viral resistance or bacterial resistance, • antibiotic resistance, • tolerance to stress, • alteration of maturation, · Modification of the content of chemical products present in the cultivated plant, preferably increase in the content of advantageous fine chemicals for applications in the field of the food industry for humans and / or feed for animals, the cosmetic industry and / or the pharmaceutical industry , • modification of the absorption of nutrients, preferably greater efficiency in the use of nutrients and / or resistance to nutrient deficiency conditions, • better fiber quality, • vigor of the plant, • color modification, • restoration of fertility, · And male sterility.
Primarily, the cultivated plants may also comprise combinations of the aforementioned traits, for example, they may be tolerant to the action of herbicides and express bacterial toxins.
Primarily, all the cultivated plants can also provide combinations of the aforementioned properties, for example, they can be tolerant to the action of herbicides and express bacterial toxins.
In the detailed description provided below, the term "plant" refers to a cultivated plant.
In one embodiment of the invention, the term "higher plant health" means an increase, compared to the respective control, of a trait selected from the group consisting of: yield (eg, higher biomass and / or yield) of seed), vigor of the plant (for example, better growth of the plant and / or early vigor and / or "effect of greater greenness", ie greener leaves, preferably leaves with a higher green index), vigor early, greater green effect (conservation of the green surface of a leaf), quality (for example, better content or composition of certain ingredients), tolerance to environmental stress, tolerance to herbicides, resistance to insects, fungal resistance or viral resistance or bacterial resistance, resistance to antibiotics, content of fine chemicals advantageous for applications in the field of the food industry for humans and / or feed for animals, the cosmetic industry or the pharmaceutical industry, efficiency in the use of nutrients, use of absorbed nutrients, quality of the fiber, color and sterility of the male and / or "greater health of the plant" means an alteration or modification, in comparison with the respective control, of a trait selected from the group consisting of maturation, restoration of fertility and color.
"Plant health" is defined as a condition of the plant that is determined by various individual or combined aspects. An indicator of the condition of the plant is its "performance".
Thus, in a preferred embodiment of the invention, the term "increased health of the plant" means an increase in performance compared to the respective control.
In one embodiment, the term "higher plant health" means any combination of 2, 3, 4, 5, 6 or more of the above-mentioned traits.
In one embodiment of the invention, the term "increased plant health" means that the same effect can be achieved in the cultivated plant as in the control plant by a reduction in application rates and / or by a reduction in the application doses.
In general, term "yield" means a measurable product of economic value, typically related to a specific crop, area and time period. The individual parts of the plants directly contribute to the yield based on their quantity, size and / or weight, or the actual yield is the yield per square meter for a crop and year, which is determined by dividing the total production (includes both the production harvested as the calculated production) per square meter planted. The term "yield" of a plant can refer to the plant biomass (root biomass and / or shoot), reproductive organs and / or propagules (such as seeds) of that plant.
In one embodiment, yield means any product of the plant of economic value produced by the plant, such as fruits, vegetables, nuts, grains, seeds, wood or even flowers. The products of the plant can be used and / or processed further after the harvest.
According to the present invention, "higher yield" of a plant, in particular of an agricultural, horticultural, silvicultural and / or ornamental plant, means that the yield of a product of the respective plant is increased by a measurable amount compared to the performance of the same product of the control plant produced under the same conditions.
In one embodiment of the invention, the higher yield is characterized, for example, by the following improved properties of the plant and / or its products compared to a control, such as greater weight, greater height, higher biomass, such as as a higher total fresh weight, higher grain yield, more shoots, larger leaves, higher growth of shoots, higher protein content, higher oil content, higher starch content and / or higher pigment content.
Another indicator of the condition of the plant is the "vigor of the plant". According to the present invention, "higher plant vigor" of a plant, in particular of an agricultural, horticultural, silvicultural and / or ornamental plant means that the vigor of a plant is increased by a measurable amount compared to the vigor of the control plant in the same conditions.
In one embodiment of the invention, the vigor of the plant is evidenced in at least one aspect selected from the group consisting of better plant vitality, better plant growth, better plant development, better visual appearance, better upright posture of the plant (lower viewing / plant inclination), better harvestability, better emergence, better nodulation, particularly rhizobial nodulation, larger size, greater leaf blade, greater weight of the plant, greater height of the plant, more shoots, higher growth of shoots, greater root growth (extensive root system), higher yield when grown in poor soils or unfavorable climate, better photosynthetic activity, better pigment content (for example, content of chlorophyll), early flowering, shorter flowering period, early fruit development, early and improved germination, early maturity of the grain, improvement in self-defense mechanisms, better stress tolerance and resistance of plants against biotic stress factors and abiotic stress, such as fungi, bacteria, viruses, insects, heat stress, cold stress, drought stress, stress UV and / or salt stress, fewer non-productive shoots, fewer dead basal leaves, fewer supplies needed (such as fertilizers, water or pesticides), greener leaves ("more green effect"), less premature maturation induced by stress and less excision of the fruits, complete maturation in shortened vegetation periods, greater and better filling of the grains, less quantity of necessary seeds, easier harvesting (for example, through the induction of leaf defoliation), faster ripening and uniform, induction of excision of young fruits (removal of small or damaged fruits so that the rest grow better "fruit thinning"), better storage, longer shelf life, simpler and more cost-effective storage conditions, larger panicles, late senescence, stronger and / or more productive shoots, better extractability of the ingredients, better quality of the seeds (to be sown and n the following stations for the production of seeds) and / or lower ethylene production and / or the inhibition of its reception by the plant in comparison with the control plant. The improvement of the vigor of the plant according to the present invention compared to the control means, in particular, that the improvement of any or several of all the above-mentioned plant characteristics improves independently of the pesticidal action of the composition or active ingredients.
"Early vigor" refers to active, healthy and balanced growth, especially during the early stages of plant growth, and may be the result of a better physical state of the plant due, for example, to the plants adapting better to their environment (that is, they optimize the use of energy resources and they are distributed between bud and root). Plants that have early vigor also show greater survival of the seedlings and better establishment of the crop, which usually results in very uniform fields (where the crop grows evenly, that is, most plants reach the various stages of development substantially at the same time), and often better and better performance. Therefore, early vigor can be determined by measuring various factors, such as weight of a thousand grains, percentage of germination, percentage of plants that emerge, seedling growth, seedling height, root length, root biomass and outbreak and many others.
Another indicator of the condition of the plant is the "quality" of a plant and / or its products.
According to the present invention, "better quality" means that certain characteristics of the crop, such as the content or composition of certain ingredients, are increased or improved by a measurable or notorious amount compared to the same factor of the control plant produced under the same conditions.
In one embodiment, the quality of a product of the respective plant is evidenced in at least one aspect selected from the group consisting of better nutrient content, better protein content, better fatty acid content, better metabolite content , better carotenoid content, better sugar content, better quantity of essential and / or non-essential amino acids, better nutrient composition, better protein composition, better fatty acid composition, better metabolite composition, better carotenoid composition, better composition of sugar, better amino acid composition, better fruit color or optimal color, better fruit texture, better leaf color, greater storage capacity and / or greater processability of the harvested products compared to the control.
In an embodiment of the invention, the quality of a product of the respective plant is revealed in at least one aspect selected from the group consisting of better nutrient yield, better protein yield, better fatty acid yield, better metabolite performance, better carotenoid yield, better sugar yield and / or better yield of essential and / or non-essential amino acids of the harvested products compared to the control. In one embodiment of the invention, the nutrient yield, protein yield, fatty acid yield, metabolite yield, carotenoid yield, sugar yield and / or yield of essential and / or non-essential amino acids is calculated as a function of the yield of seed and / or biomass in relation to the respective nutrient, protein, fatty acid, metabolite, carotenoid, sugar and / or essential and / or non-essential amino acids.
The terms "increase", "improvement" or "increase" can be used indistinctly and mean, in the sense of the request, at least 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%, preferably at least 15% or 20%, more preferably at least 25%, 30%, 35% or 40% more of the respective trait, characteristic, appearance, property , particularity or attribute described in the present specification, for example, yield and / or growth in comparison with the control plants as defined herein.
In one embodiment of the invention, the highest yield of the seed is manifested as one or more of the following: a) increase in seed biomass (total seed weight) that can be considered on the basis of each seed and / or per floor and / or per square meter; b) more flowers per plant; c) more seeds (full); d) higher seed filling rate (expressed as the ratio between the number of filled seeds divided by the total number of seeds); e) higher harvest index, which is expressed as the proportion of the yield of the harvestable parts, such as seeds, divided by the total biomass; and f) greater thousand kernel weight (TKW), which is extrapolated from the number of full seeds counted and their total weight. A higher TKW may be the result of a larger seed size and / or seed weight, and may also result from a larger embryo and / or endosperm size.
In an embodiment of the invention, the increase in the yield of the seeds also manifests itself as an increase in the size of the seed and / or in the volume of the seed. Likewise, an increase in seed yield also manifests as an increase in the seed area and / or seed length and / or seed width and / or seed perimeter. In another embodiment, the higher performance also results in a modified architecture, or may be the result of a modified architecture.
In one embodiment, the beneficial effect of the present invention may be manifested not in the seed yield per se, but in the quality of the seed and in the quality of the agricultural products produced from the plants treated in accordance with the invention. The quality of the seed can refer to different parameters known in the art, such as better nutrients or better content of fine chemical, for example, amount of vitamins or fatty acids and their composition; color or shape of the seed; germination or vigor of the seed; or lesser amount of toxins, for example, fungal toxins and / or substances difficult to digest or that can not be digested, for example, phytate, lignin.
The "greenness index" as used herein is calculated from digital images of plants. For each pixel that belongs to the plant object of the image, the proportion of the value of green with respect to the value of red is calculated (in the RGB model for the color coding). The green index is expressed as the percentage of pixels for which the green-red ratio exceeds a certain threshold. Under normal growing conditions, under growing conditions with salt stress and under growing conditions with reduced availability of nutrients, the greenness index of the plants is measured in the last formation of images before flowering. On the contrary, in conditions of growth with drought stress, the greenness index of the plants is measured in the first image formation after the drought. Similarly, measurements can be made after exposure to other treatments with abiotic stress, for example, temperature.
Another indicator of the condition of the plant is the tolerance or resistance of the plant to the biotic and / or abiotic stress factors. Biotic and abiotic stress, especially over prolonged periods, can have detrimental effects on plants. Biotic stress is caused by living organisms, while abiotic stress is caused, for example, by extreme environmental conditions or unfavorable for the optimal growth of the plant.
According to the present invention, "better tolerance or resistance to biotic and / or abiotic stress factors" means (1.) that certain negative factors caused by biotic and / or abiotic stress decrease by a measurable or remarkable amount compared to the control plants exposed to the same conditions and (2) that the negative effects are not diminished by the direct action of the composition on the stress factors, for example, by their fungicidal or insecticidal action, which directly destroys the microorganisms or the pests, but by a stimulation of the defensive reactions of the plant ("priming") against said stress factors ("induced resistance") or by the aforementioned synergistic effect.
Biotic stress can be caused by living organisms, such as pests (eg, insects, arachnids, nematodes), competing plants (eg, weeds), microorganisms (such as phytopathogenic fungi and / or bacteria) and / or viruses. Abiotic stress can be caused, for example, by extreme temperatures such as heat or cold (heat stress, cold stress), marked temperature variations, unusual temperatures for a certain season, drought (drought stress), extreme humidity, high salinity (stress due to salinity), radiation (for example, due to increased UV radiation due to the reduction of the ozone layer), higher levels of ozone (ozone stress), organic pollution (for example, phytotoxic amounts of pesticides) and by inorganic pollution (for example, by heavy metal contaminants). The factors of biotic and abiotic stress can also cause secondary stress types, such as oxidative stress.
As a result of biotic and / or abiotic stress factors, the quantity and quality of stressed plants, their crops and fruits decrease.
In one embodiment of the invention, the best tolerance or biotic resistance of the respective plant is manifested in at least one aspect selected from the group consisting of tolerance or resistance to pests (eg, insects, arachnids, nematodes), plants competing (for example, weeds), microorganisms (such as phytopathogenic fungi and / or bacteria) and / or viruses.
In one embodiment of the invention, the best tolerance or abiotic resistance of the respective plant is manifested in at least one aspect selected from the group consisting of tolerance or resistance to extreme temperatures such as heat or cold (heat stress, stress by cold), marked temperature variations, unusual temperatures for a certain season, drought (drought stress), extreme humidity, high salinity (stress due to salinity), radiation (for example, due to increased UV radiation due to the reduction of the layer of ozone), higher levels of ozone (stress due to ozone), organic pollution (for example, by phytotoxic amounts of pesticides) and inorganic pollution (for example, by heavy metal pollutants).
The indicators identified above for the health condition of a plant can be interdependent and can be the result of one another. For example, a higher resistance to biotic and / or abiotic stress can lead to a better vigor of the plant, for example, to better and bigger crops and, in this way, to a higher yield. On the contrary, a more developed root system can give as result greater resistance to biotic and / or abiotic stress. However, these interdependencies and interactions are not fully known or fully understood.
In one embodiment of the present invention, the yield of the plant increases with increasing tolerance to environmental stress of a plant, in particular, tolerance to abiotic stress. In general, the term "greater stress tolerance" can be defined as plant survival and / or higher yield yield under stress conditions, as compared to a control plant: For example, the plant of the invention is better adapted to the stress conditions. "Better adaptation" to environmental stresses, such as drought, heat, nutrient depletion, freezing temperatures and / or frost, refers to a better performance of the plant that results in higher yields, in particular , with respect to one or more of the features related to performance, as defined in greater detail above.
During its life cycle, a plant usually faces various environmental conditions. Any of these conditions, which may have, in certain circumstances, an impact on the performance of the plant, are referred to in the present "stress" condition. The types of environmental stress can generally be divided into biotic and abiotic (environmental) stress. Unfavorable nutrient conditions are sometimes referred to as "environmental stress". In one embodiment, the present invention also contemplates solutions for this type of environmental stress, for example, in relation to greater efficiency in the use of nutrients.
For the purposes of the description of the present invention, the terms "better tolerance to stress", "better resistance to environmental stress", "better tolerance to environmental stress", "better adaptation to environmental stress" and other similar variations and expressions in their meaning are used indistinctly and refer, without limitation, to a better tolerance to one or more types of environmental stress, as described herein, and in comparison with a corresponding control plant.
The term tolerance to abiotic stress refers, for example, tolerance to low temperature, tolerance to drought or better efficiency in the use of water (WUE), tolerance to heat, tolerance to stress by salinity and others. The stress tolerance of plants, such as stress tolerance due to low temperature, drought, heat or salinity, can have an important common point for plant growth, namely, the availability of water. During their life cycle, plants are typically exposed to conditions where the environmental water content is reduced. The protection strategies are similar to those of frost tolerance.
Accordingly, in one embodiment of the present invention, said feature related to performance refers to a greater efficiency in the use of water of the plant of the invention and / or a greater tolerance to drought conditions of the plant of the invention. the invention. Water use efficiency (WUE) is a parameter that is often correlated with tolerance to drought. An increase in biomass in conditions of low water availability can be due to relatively improved growth efficiency or low water consumption. When selecting traits to improve crops, a decrease in water use, without a change in growth, would be particularly important in an irrigated agricultural system in which water costs are high. An increase in growth without a corresponding increase in water use would be applicable in all agricultural systems. In many agricultural systems in which the water supply is not limiting, an increase in growth, even if it is at the expense of an increase in water use, also increases yield.
In an embodiment of the present invention, a higher yield of the plant is achieved by increasing the "efficiency in the use of nutrients of a plant", for example, by improving the efficiency in the use of nutrients that include, but not they are limited to, phosphorus, potassium and nitrogen. In one embodiment, a greater efficiency in the use of nutrients is a better absorption, assimilation, accumulation or utilization of nitrogen. These complex processes are associated with the absorption, translocation, assimilation and redistribution of nitrogen in the plant.
It has been pointed out that the aforementioned effects of the method according to the invention, that is, better health of the plant, are also present when the plant is not under conditions of biotic stress, for example, when the plant is not under fungi pressure or pests. It is evident that a plant that suffers the attack of fungi or insects produces lower biomass and lower crop yield in comparison with a plant that has been subjected to a preventive or curative treatment against the plague or the pathogenic fungus and that can grow without the damage caused by the biotic stress factor. However, the method according to the invention leads to a better health of the plant, even in the absence of any biotic stress and, in particular, of any pest or phytopathogenic fungus. This means that the positive effects of the method of the invention can not be explained solely by the activity pesticide of the compounds of the invention, but are based on other activity profiles.
The term "plant", as used herein, encompasses whole plants and their progeny, and parts of plants, including seeds, shoots, stems, leaves, roots (including tubers), flowers and tissues, and organs.
For the purposes of the invention, as a rule, the plural is intended to encompass the singular and vice versa.
Tolerance to herbicides can be achieved by creating desensitization at the site of action of the herbicide by the expression of a white enzyme that is resistant to the herbicide; rapid metabolization (conjugation or degradation) of the herbicide by the expression of enzymes that inactivate the herbicide; or poor absorption and translocation of the herbicide. Examples are the expression of enzymes that are tolerant to the herbicide compared to the wild-type enzymes, such as the expression of 5-enolpyruvylshikimato-3-phosphate synthase (EPSPS), which is glyphosate tolerant (see, for example, Heck et al. al, Crop Sci 45, 2005, 329-339, Funke et al, PNAS 103, 2006, 13010-13015, US 5188642, US 4940835, US 5633435, US 5804425, US 5627061), the expression of glutamine synthase which is tolerant to glufosinate and bialaphos (see, for example, US 5646024, US 5561236) and DNA constructs encoding enzymes that degrade dicamba (see, for example, US 7105724). Gene constructs can be obtained, for example, from microorganisms or plants, which are tolerant to said herbicides, such as the CP4 EPSPS strain of Agrobacterium which is resistant to glyphosate; Streptomyces bacteria that are resistant to glufosinate; Arabidopsis, Daucus carota, Pseudomonoas spp. or Zea mais with chimeric gene sequences encoding HDDP (see, for example, WO 1996/38567, WO 2004/55191); Arabidopsis thaliana which is resistant to protox inhibitors (see, for example, US 2002/0073443).
Preferably, the herbicide tolerant plant can be selected from cereals such as wheat, barley, rye, oats; cañola, sorghum, soybeans, rice, oilseed rape, sugar beet, sugarcane, grape, lentil, sunflower, alfalfa, pomo fruits; stone fruits; peanut; coffee; tea; strawberries; grass; vegetables, such as tomatoes, potatoes, cucurbits and lettuce, more preferably, the plant is selected from soybeans, corn, rice, cotton, oilseed rape, in particular cañola, tomatoes, potatoes, sugar cane and cereals such as wheat, barley, rye and oats.
Examples of commercially available transgenic plants with herbicide tolerance are the "Roundup Ready Corn", "Roundup Ready 2" (Monsanto), "Agrisure GT", "Agrisure GT / CB / LL", "Agrisure GT" / RW "," Agrisure 3000GT "(Syngenta)," YieldGard VT Rootworm / RR2"and" YieldGard Triple VT "(Monsanto) with tolerance to glyphosate; the corn varieties "Liberty Link" (Bayer), "Herculex I", "Herculex RW", "Herculex Xtra" (Dow, Pioneer), "Agrisure GT / CB / LL" and "Agrisure CB / LL / RW" ( Syngenta) with tolerance to glufosinate; the soybean varieties "Roundup Ready Soybean" (Monsanto) and "Optimum GAT" (DuPont, Pioneer) with tolerance to glyphosate; the varieties of cotton "Roundup Ready Cotton" and "Roundup Ready Flex" (Monsanto) with tolerance to glyphosate; the "FiberMax Liberty Link" cotton variety (Bayer) with tolerance to glufosinate; the cotton variety "BXN" (Calgene) with tolerance to bromoxynil; Canola varieties "Navigator" and "Compass" (Rhone-Poulenc) with tolerance to bromoxynil; the variety of cañola "Roundup Ready Cañóla" (Monsanto) with tolerance to glyphosate; the variety of canola "InVigor" (Bayer) with tolerance to glufosinate; the variety of rice "Liberty Link Rice" (Bayer) with tolerance to glyphosate and the alfalfa variety "Roundup Ready Alfalfa" with tolerance to glyphosate. Other transgenic plants with tolerance to herbicides are commonly known, for example, alfalfa, apple, eucalyptus, flax, grape, lentil, oilseed rape, peas, potato, rice, sugar beet, sunflower, tobacco, tomato, turf and wheat with tolerance to glyphosate (see, for example, US 5188642, US 4940835, US 5633435, US 5804425, US 5627061); beans, soybeans, cotton, peas, potatoes, sunflowers, tomatoes, tobacco, corn, sorghum and sugarcane with tolerance to dicamba (see, for example, US 7105724 and US 5670454); pepper, apple, tomato, millet, sunflower, tobacco, potato, corn, cucumber, wheat and sorghum with tolerance to 2,4-D (see, for example, US 6153401, US 6100446, WO 2005107437, US 5608147 and US 5670454); sugar beet, potato, tomato and tobacco with tolerance to glufosinate (see, for example, US 5646024, US 5561236); cañola, barley, cotton, lettuce, melon, millet, oats, potatoes, rice, rye, sorghum, soybeans, sugar beet, sunflower, tobacco, tomato and wheat with tolerance to herbicides acetolactate synthase inhibitors (ALS), such as triazolopyrimidin sulfonamides , sulfonylureas and imidazolinones (see, for example, US 5013659, WO 2006060634, US 4761373, US 5304732, US 6211438, US 621 1439 and US 6222100); cereals, sugarcane, rice, corn, tobacco, soya, cotton, rapeseed, sugar beet and potato with tolerance to HPPD inhibitor herbicides (see, for example, WO 2004/055191, WO 199638567, WO 1997049816 and US 6791014); wheat, soybean, cotton, sugar beet, rapeseed, rice, sorghum and sugar cane with tolerance to protoporphyrinogen oxidase (PPO) inhibiting herbicides (see, for example, US 2002/0073443, US 20080052798, Pest Management Science, 61, 2005 , 277-285). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications.
Plants that are capable of synthesizing one or more bacterial toxins that act selectively comprise, for example, at least one toxin of the toxin-producing bacteria, especially those of the genus Bacillus, in particular plants capable of synthesizing one or more Bacillus insecticidal proteins. cereus or Bacillus popliae; or Bacillus thuringiensis insecticidal proteins, such as delta. endotoxins, for example, CrylA (b), CrylA (c), CryIF, CrylF (a2), CryllA (b), CryIIIA, CrylNB (bl) or Cry9c, or vegetative insecticidal proteins (VIP), for example, VIP1, VIP2, VIP3 or VIP3A; or insecticidal proteins from bacteria-colonizing nematodes, for example, Photorhabdus spp. or Xenorhabdus spp., such as Photorhabdus luminescens, Xenorhabdus nematophilus; toxins produced by animals, such as scorpion toxins, arachnid toxins, wasp toxins and other insect-specific neurotoxins; toxins produced by fungi, such as Streptomycetes toxins, plant lectins, such as peas lectins, barley lectins or winter chime lectins; agglutinins; proteinase inhibitors, such as trypsin inhibitors, serine protease inhibitors, patatin inhibitors, cystatin, papain; proteins that inactivate ribosomes (RIP), such as ricin, corn RIP, abrin, lufina, saporin or bryodin; Enzymes of steroid metabolism, such as 3-hydroxysterodeoxydase, ecdysteroid-UDP-glycosyl-transferase, cholesterol oxidases, ecdysone inhibitors, HMG-COA-reductase, ion channel blockers, such as sodium or calcium channel blockers , esterase of juvenile hormone, receptors of diuretic hormone, stilbene synthase, bibencil synthase, chitinases and glucanases.
In one embodiment, a plant is capable of producing toxin, lectin or inhibitor if it contains at least one cell comprising a nucleic acid sequence encoding said toxin, lectin, inhibitor or enzyme that produces inhibitors, and said nucleic acid sequence it is transcribed and translated and, if appropriate, the resulting protein is processed and / or secreted constitutively or subjected to the development, inducible or tissue-specific regulation.
In the context of the present invention, the delta should be interpreted.- endotoxins, for example, CrylA (b), Cr lA (c), CrylF, CrylF (a2), CryllA (b), CryIIIA, CrylllB (bl) or Cry9c, or vegetative insecticidal proteins (VIP), eg, VIP1, VIP2, VIP3 or VIP3A, specifically also hybrid toxins, truncated toxins and modified toxins. The hybrid toxins are produced recombinantly by a new combination of different domains of those proteins (see, for example, WO 02/15701). An example of a truncated toxin is a truncated CrylA (b), which is expressed in the Bt11 maize of Syngenta Seed SAS, as described below. In the case of the modified toxins, one or more amino acids of the natural toxin are replaced. In said amino acid replacements, protease recognition sequences present in an unnatural form are preferably inserted into the toxin, such as, for example, in the case of CrylllA055, a cathepsin-D recognition sequence is inserted into a CryIIIA toxin ( see, WO 2003/018810).
Examples of said toxins or transgenic plants capable of synthesizing said toxins are described, for example, in EP-A-0 374 753, WO 93/07278, WO 95/34656, EP-A-0 427 529, EP-A- 451 878 and WO 2003/052073.
The processes for the preparation of said transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications. Cryl-type deoxyribonucleic acids and their preparation are known, for example, from WO 95/34656, EP-A-0 367 474, EP-A-0 401 979 and WO 1990/13651.
The toxin contained in transgenic plants gives plants tolerance to harmful insects. These insects can be present in any taxonomic group of insects, but it is common to find them especially in beetles (Coleoptera), two-winged insects (Diptera) and butterflies (Lepidoptera).
Preferably, the plant that can express bacterial toxins is selected from cereals such as wheat, barley, rye, oats; cañola, cotton, eggplant, lettuce, sorghum, soybean, rice, oilseed rape, sugar beet, sugarcane, grape, lentil, sunflower, alfalfa, pomo fruits; stone fruits; peanut; coffee; tea; strawberries; grass; vegetables, such as tomatoes, potatoes, cucurbits and lettuce, more preferably, the plant is selected from cotton, soybeans, corn, rice, tomatoes, potatoes, oilseed rape and cereals such as wheat, barley, rye and oats, most preferably cotton , soy, corn and cereals such as wheat, barley, rye and oats.
Examples of commercially available transgenic plants capable of expressing the bacterial toxins are the corn varieties "YieldGard com rootworm" (Monsanto), "YieldGard VT" (Monsanto), "Herculex RW" (Dow, Pioneer), "Herculex Rootworm" (Dow, Pioneer) and "Agrisure CRW" (Syngenta) with resistance to corn rootworm; the corn varieties "YieldGard corn borer" (Monsanto), "YieldGard VT Pro" (Monsanto), "Agrisure CB / LL" (Syngenta), "Agrisure 3000GT" (Syngenta), "Hercules I", "Hercules II" ( Dow, Pioneer), "KnockOut" (Novartis), "NatureGard" (Mycogen) and "StarLink" (Aventis) with resistance to corn borer, the corn varieties "Herculex I" (Dow, Pioneer) and "Herculex Xtra" (Dow, Pioneer) with resistance to western bean cutworm, corn borer, black cutworm and autumn leafworm, corn variety "YieldGard Plus" (Monsanto) with resistance to corn borer and rootworm of corn; the variety of cotton "Bollgard I" "(Monsanto) with resistance to the worm of the tobacco bud, the cotton varieties" Bollgard II "(Monsanto)," WideStrike "(Dow) and" VipCot "(Syngenta) with resistance to the worm from tobacco bud, cotton bollworm, autumn budworm, beet bollworm, cabbage bollworm, soybean worm and pink bollworm, potato varieties "NewLeaf," NewLeaf Y "and" NewLeaf Plus "(Monsanto) with resistance to tobacco horn worm and eggplant varieties" Bt brinjal "," Dumaguete Long Purple "," Mará "with resistance to shoot borer and fruits of eggplant, borer fruits and worm of the cotton bud (see, for example, US5128130). Other transgenic plants with insect resistance are commonly known, such as rice resistant to yellow-stem borer (see, eg, Molecular Breeding, Volume 18, 2006, Number 1), lepidoptera-resistant lettuce (see, for example, US 5349124). ), resistant soybeans (see, for example, US 7432421) and Lepidoptera resistant rice, such as rice stem borer, rice borer worm, rice cutter worm, rice caddis worm, rice leaf winger and rice armyworm (see, for example, WO 2001021821). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications.
Preferably, the plants that are capable of synthesizing antipathogenic substances are selected from soy, corn, rice, tomato, potato, banana, papaya, tobacco, grape, plum and cereals such as wheat, barley, rye and oats, most preferably soy. , corn, rice, cotton, tomato, potato, banana, papaya, rapeseed oleaginous and cereals such as wheat, barley, rye and oats.
Plants that are capable of synthesizing antipathogenic substances that have a selective action are, for example, plants that express the so-called "pathogenesis-related proteins" (PRP, see, for example, EP-A-0 392 225) or the so-called " antifungal proteins "(AFP, see, for example, US 6864068). Several antifungal proteins with activity against the plant pathogenic fungi have been isolated from certain plant species and are of general knowledge. Examples of said antipathogenic substances and transgenic plants capable of synthesizing said antipathogenic substances are known, for example, from EP-A-0 392 225, WO 93/05153, WO 95/33818 and EP-A-0 353 191. The plants Transgenic plants that are resistant to fungal, viral and bacterial pathogens are produced by the introduction of resistant plant genes. Numerous resistant genes have been identified, isolated and used to improve plant resistance, such as the N gene that was introduced into lines of tobacco susceptible to the Tobacco Mosaic Virus (TMV) in order to produce tobacco plants resistant to TMV ( see, for example, US 5571706), the Prf gene, which was introduced into plants to obtain better resistance to pathogens (see, for example, WO 199802545) and the Rps2 gene from Arabidopsis thaliana, which was used to create resistance to bacterial pathogens, including Pseudomonas syringae (see, for example, WO 199528423). Plants were obtained that exhibited a systemic acquired resistance response by introducing a nucleic acid molecule encoding the TIR domain of the N gene (see, for example, US 6630618). Other examples of known resistant genes are the Xa21 gene, which was introduced into several rice cultivars (see, for example, US 5952485, US 5977434, WO 1999/09151, WO 1996/22375), the Regi gene for resistance to colletotrichum ( see, for example, US 2006/225152), the prp1 gene (see, for example, US 5859332, WO 2008/017706), the ppv-cp gene for providing resistance to plum pox virus (see, for example, US Pat. PP15,154Ps), the P1 gene (see, for example, US5968828), genes such as Blb1, Blb2, Blb3 and RB2 to provide resistance to Phytophthora infestans in potato (see, for example, US 7148397), the LRPKml gene (see , for example, W01999064600), the P1 gene for potato Y virus resistance (see, for example, US 5968828), the HA5-1 gene (see, for example, US5877403 and US6046384), the PIP gene to provide a broad resistance to viruses, such as potato virus X (PVX), potato Y virus (PVY), potato leafroller virus (PLRV) (see, for example) Example, EP 0707069) and genes such as the N116, ScaM4 and ScaM5 genes of Arabidopsis to obtain fungal resistance (see, for example, US 6706952 and EP 1018553). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications.
Antipathogenic substances that can be expressed by said transgenic plants include, for example, ion channel blockers, such as sodium and calcium channel blockers, for example, viral toxins KP1, KP4 or KP6; stilbeno synthases; bibencil sintasas; chitinases; glucanases; the so-called "pathogenesis-related proteins" (PRP, see, for example, EP-A-0 392 225); antipathogenic substances produced by microorganisms, for example, peptide antibiotics or heterocyclic antibiotics (see, for example, WO 1995/33818) or polypeptide or protein factors involved in the defense of the plant against pathogens (so-called "resistance genes"). plant diseases ", as described in WO 2003/000906).
The antipathogenic substances produced by the plants are able to protect them against various pathogens, such as fungi, viruses and bacteria. Useful plants of great interest related to the present invention are cereals, such as wheat, barley, rye and oats; soy; corn; rice; alfalfa, cotton, sugar beet, sugar cane, tobacco, potato, banana, oilseed rape; pomo fruits; stone fruits; peanut; coffee; tea; strawberries; grass; vines and vegetables, such as tomatoes, potatoes, cucurbits, papaya, melon, lentils and lettuce, are more preferably selected from soybean, corn, alfalfa, cotton, potato, banana, papaya, rice, tomato and cereals such as wheat, barley, rye and oats, more preferably soy, corn, rice, cotton, potato, tomato, oilseed rape and cereals such as wheat, barley, rye and oats.
Transgenic plants resistant to fungal pathogens are, for example, soybean with resistance to Asian soybean rust (see, for example, WO 2008/017706); plants such as alfalfa, corn, cotton, sugar beet, rapeseed, tomato, soybean, wheat, potato and tobacco with resistance to Phytophtora infestans (see, for example, US5859332, US 7148397, EP 1334979); corn with resistance to leaf blights, spindle rot and stem rot (such as anthracnose leaf blight, anthracnose stem rot, diplodia ear rot, Fusarum verticilioides, Gibberella zeae, and dieback, see, for example, US 2006/225152); apples with resistance to apple scab (Venturia inaequalis, see, for example, WO 1999064600); plants such as rice, wheat, barley, rye, corn, oats, potato, melon, soybean and sorghum with resistance to fusarium diseases, such as Fusarium graminearum, Fusarium sporotrichioides, Fusarium lateritium, Fusarium pseudograminearum Fusarium sambucinum, Fusarium culmorum, Fusarium poae, Fusarium acuminatum, Fusarium equiseti (see, for example, US 6646184, EP 1477557); plants, such as corn, soybeans, cereals (in particular wheat, rye, barley, oats, rye, rice), tobacco, sorghum, sugar cane and potato with a broad resistance to fungi (see, for example, US 5689046, US Pat. 6706952, EP 1018553 and US 6020129).
Transgenic plants resistant to bacterial pathogens and which are included in the present invention are, for example, rice with resistance to Xylella fastidiosa (see, for example, US 6232528); plants, such as rice, cotton, soybean, potato, sorghum, corn, wheat, barley, sugar cane, tomato and pepper, with resistance to bacterial blight (see, for example, WO 2006/42145, US 5952485, US 5977434, WO 1999/09151, WO 1996/22375); tomato with resistance to Pseudomonas syringae (see, for example, Can. J. Plant Path., 1983, 5: 251-255).
Transgenic plants resistant to viral pathogens are, for example, stone fruits, such as plum, almond, damask, cherry, peach, nectarine, with resistance to pox virus of the plum (PPV, see, for example, US PP15,154Ps , EP 0626449); potatoes with resistance to potato virus Y (see, for example, US 5968828); plants such as potato, tomato, cucumber and legumes that are resistant to tomato dotted wilt virus (TSWV, see, for example, EP 0626449, US 5973135); corn with resistance to corn striatum virus (see, for example, US 6040496); papaya with resistance to the papaya ring spot virus (PRSV, see, for example, US 5877403, US 6046384); cucurbitaceae, such as cucumber, melon, watermelon and squash, and solanaceous, such as potato, tobacco, tomato, eggplant, paprika and pepper, with resistance to cucumber mosaic virus (CMV, see, for example, US 6849780); cucurbitaceae, such as cucumber, melon, watermelon and squash, with resistance to watermelon mosaic virus and zucchini yellow mosaic virus (see, for example, US 6015942); potatoes with resistance to potato leaf roll virus (PLRV, see, for example, US 5576202); potatoes with broad resistance to viruses, such as potato virus X (PVX), potato virus Y (PVY), potato leaf roll virus (PLRV) (see, for example, EP 0707069).
Table I: Other examples of deregulated or commercially available transgenic plants with modified genetic material capable of expressing antipathogenic substances are Transgenic plants with resistance to nematodes and which can be used in the methods of the present invention are, for example, soybean plants with resistance to soybean cyst nematodes.
Methods for the genetic transformation of plants have been proposed in order to confer greater resistance to the parasitic nematodes of the plant. U.S. Patent Nos. 5,589,622 and 5,824,876 relate to the identification of plant genes expressed specifically at or adjacent to the plant's feeding site after the nematode has been subjected to it.
Also known in the art are transgenic plants with reduced feeding structures for parasitic nematodes, for example, herbicide-resistant plants, except for those parts or those cells which are feeding sites of the nematode, and treatment of said plant with a herbicide. to prevent, reduce or limit the feeding of the nematode by damaging or destroying the feeding sites (for example, US 5866777).
The use of RNAi to label essential nematode genes has been proposed, for example, in PCT publication 2001/96584, WO 2001/17654, US 2004/0098761, US 2005/0091713, US 2005/0188438, US 2006/0037101 , US 2006/0080749, US 2007/0199100 and US 2007/0250947.
Transgenic plants resistant to nematodes have been described, for example, in PCT publication WO 2008/095886 and WO 2008/095889.
Plants that are resistant to antibiotics, such as kanamycin, neomycin and ampicillin. The natural bacterial gene nptll expresses the enzyme that blocks the effects of the antibiotics kanamycin and neomycin. The ampicillin resistance gene ampR (also known as blaTEMI) is derived from the bacterium Salmonella paratyphi and is used as a marker gene in the transformation of microorganisms and plants. It is responsible for the synts of the beta-lactamase enzyme, which neutralizes antibiotics in the penicillin group, including ampicillin. Transgenic plants with resistance to antibiotics are, for example, potato, tomato, flax, canola, oilseed rape and corn (see, for example, Plant Cell Reports, 20, 2001, 610-615.) Trends in Plant Science, 1 1, 2006, 317-319, Plant Molecular Biology, 37, 1998, 287-296, Mol Gen Genet., 257, 1998, 606-13.). Plant Cell Reports, 6, 1987, 333-336. Federal Register (USA), Vol.60, No.1 13, 1995, page 31 139. Federal Register (USA), Vol.67, No.226, 2002, page 70392. Federal Register (USA), Vol.63, No.88, 1998, page 25194. Federal Register (USA), Vol.60, No.141, 1995, page 37870. Canadian Food Inspection Agency, FD / OFB-095-264-A, October 1999, FD / OFB- 099-127-A, October 1999. Preferably, the plant is selected from soy, corn, rice, cotton, oilseed rape, potato, sugarcane, alfalfa, tomato and cereals, such as wheat, barley, rye and oats, with greater preference of soy, corn, rice, cotton, oilseed rape, tomato, potato and cereals such as wheat, barley, rye and oats.
Plants that are tolerant to stress conditions (see, for example, WO 2000/04173, WO 2007/131699, CA 2521729 and US 2008/0229448) are plants that show greater tolerance to abiotic stress conditions such as drought, high salinity, high intensity of light, high UV irradiation, chemical pollution (such as heavy metal concentrations), low or high temperatures, limited supply of nutrients (ie, nitrogen, phosphorus) and population stress. Preferably, transgenic plants with resistance to stress conditions are selected from rice, corn, soybeans, sugar cane, alfalfa, wheat, tomato, potato, barley, rapeseed, beans, oats, sorghum and cotton with tolerance to drought (see , for example WO 2005/048693, WO 2008/002480 and WO 2007/030001); corn, soybeans, wheat, cotton, rice, rapeseed and alfalfa with tolerance to low temperatures (see, for example, US 4731499 and WO 2007/1 12122); rice, cotton, potato, soybean, wheat, barley, rye, sorghum, alfalfa, grape, tomato, sunflower and tobacco with tolerance to high salinity (see, for example, US 7256326, US 7034139, WO 2001/030990). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications. Preferably, the plant is selected from soya, corn, rice, cotton, sugarcane, alfalfa, sugar beet, potato, oilseed rape, tomato and cereals such as wheat, barley, rye and oats, with greater preference of soybeans, corn, rice, cotton, oilseed rape, tomato, potato, sugar cane and cereals such as wheat, barley, rye and oats.
The altered ripening properties are, for example, late ripening, late softening and early ripening. Preferably, the transgenic plants with modified ripening properties are selected from tomato, melon, raspberry, strawberry, muskmelon, pepper and papaya with late ripening (see, for example, US 5767376, US 7084321, US 6107548, US 5981831, WO 1995035387, US 5952546, US 5512466, WO 1997001952, WO 1992/008798, Plant Cell, 1989, 53-63, Plant Molecular Biology, 50, 2002). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications. Preferably, the plant is selected from fruits, such as tomato, vine, melon, papaya, banana, pepper, raspberry and strawberry; stone fruits, such as cherry, damask and peach; pomo fruits, such as apple and pear; and citrus fruits, such as French lemon, lime, orange, grapefruit, grapefruit and tangerine, with a greater preference of tomato, vine, apple, banana, orange and strawberry, most preferably tomatoes.
The modification of the content is the synthesis of the modified chemical compounds (in comparison with the corresponding control plant) or the synthesis of improved quantities of chemical products (if they are compounds compared to the corresponding control plant) and corresponds to a greater or lower amount of vitamins, amino acids, proteins and starch, different oils and a smaller amount of nicotine.
The commercial examples are the soy varieties "Vistive II" and "Visitive III" with a low-linolenic / oleic content; the "Mavera high-value corn" variety with the highest lysine content; and the soy variety "Mavera high value soybean" that yields 5% more protein compared to conventional varieties when processed and transformed into soy flour. Other transgenic plants with altered content are, for example, potato and corn with modified amylopectin content (see, for example, US 6784338, US 20070261136); cañola, corn, cotton, grape, catalpa, cattail, rice, soy, wheat, sunflower, bitter melon and vernonia with modified oil content (see, for example, US 7294759, US7157621, US 5850026, US 6441278, US 6380462, US 6365802, US 6974898, WO 2001/079499, US 2006/0075515 and US 7294759); sunflower with higher content of fatty acids (see, for example, US 6084164); soybean with modified allergen content (so-called "hypoallergenic soybean, see, for example, US 6864362), tobacco with reduced nicotine content (see, for example, US 20060185684, WO 2005000352 and WO 2007064636), canola and soybean with higher content of lysine (see, for example, Bio / Technology 13, 1995, 577-582); maize and soybean with altered composition of methionine, leucine, isoleucine and valine (see, for example, US 6946589, US 6905877); sulphurated amino acids (see, for example, EP 0929685, WO 1997041239), tomato with higher content of free amino acids, such as asparagine, aspartic acid, serine, threonine, alanine, histidine and glutamic acid (see, for example US 6727411); with better amino acid content (see, for example, WO 050771 17), potatoes, corn and rice with modified starch content (see, for example, WO 1997044471 and US 7317146), tomato, corn, grape, alfalfa, apple, beans and peas with content modified flavonoids (see, for example, WO 2000/04175); corn, rice, sorghum, cotton, soybean with altered content of phenolic compounds (see, for example, US 20080235829). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications. Preferably, the plant is selected from soya, corn, rice, cotton, sugar cane, potato, tomato, oilseed rape, flax and cereals such as wheat, barley, rye and oats, most preferably soy, corn, rice, oilseed rape. , potatoes, tomatoes, cotton and cereals such as wheat, barley, rye and oats.
A better utilization of nutrients is, for example, the assimilation or metabolization of nitrogen or phosphorus. Preferably, the transgenic plants with a capacity for better assimilation and utilization of nitrogen are selected, for example, from cañola, corn, wheat, sunflower, rice, tobacco, soybeans, cotton, alfalfa, tomato, wheat, potato, sugar beet, cane of sugar and rapeseed (see, for example WO 1995/00991 1, WO 1997/030163, US 6084153, US 5955651 and US 6864405). Plants with better phosphorus absorption are, for example, tomato and potato (see, for example, US 7417181). The methods for producing said transgenic plants are generally known to the skilled in the art and are described, for example, in the aforementioned publications. Preferably, the plant is selected from soya, corn, rice, cotton, sugarcane, alfalfa, potato, oilseed rape and cereals such as wheat, barley, rye and oats, most preferably soy, corn, rice, cotton, rapeseed. oilseed, tomato, potato and cereals such as wheat, barley.
Transgenic plants with male sterility are preferably selected from barley, corn, tomato, rice, Indian mustard, wheat, soybean and sunflower (see, for example, US 6720481, US 6281348, US 5659124, US 6399856, US 7345222, US 7230168 , US 6072102, EP1 135982, WO 2001/092544 and WO 1996/040949). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications. Preferably, the plant is selected from soya, corn, rice, cotton, oilseed rape, tomato, potato and cereals such as wheat, barley.
Table II: Other examples of transgenic plants commercially available or deregulated with modified genetic material that is sterile in the male are Plants that produce higher quality fiber are, for example, plants of transgenic cotton Said fiber of better quality refers to a better micronaire of the fiber, greater strength, better length of the fiber, better uniformity in length and color of the fibers (see, for example, WO 1996/26639, US 7329802, US 6472588 and WO 2001/17333). The methods for producing such transgenic plants are generally known to the person skilled in the art and are described, for example, in the aforementioned publications.
As indicated above, the cultivated plants may comprise one or more traits, for example, selected from the group consisting of herbicide tolerance, insect resistance, fungal resistance, viral resistance, bacterial resistance, stress tolerance, alteration of maturation, modification of the content, modification of the absorption of nutrients and sterility of the male (see, for example, WO 2005033319 and US 6376754).
Examples of commercially available transgenic plants with two combined properties are the "YieldGard Roundup Ready" and YieldGard Roundup Ready 2"(Monsanto) corn varieties with glyphosate tolerance and corn borer resistance, the" Agrisure CB "variety of corn. / LL "(Syntenta) with tolerance to glufosinate and resistance to corn borer, corn variety" Yield Gard VT Rootworm / RR2"with glyphosate tolerance and resistance to corn rootworm, corn variety" Yield Gard Triple VT "with tolerance to glyphosate and resistance to corn rootworm and corn borer, the variety of corn" Herculex I "with tolerance to glufosinate and resistance to lepidoptera (CryI F), that is, against the cutworm of the western bean, corn borer, black cutworm and autumn fall worm, the corn variety "YieldGard Corn Rootworm / Roundup Ready 2" (Monsanto) with glyphosate tolerance and worm resistance or from the root of corn; the variety of corn "Agrisure GT / RW" (Syngenta) with tolerance to glufosinate and resistance to lepidoptera (Cry3A), that is, against the root worm of western corn, rootworm of northern corn and worm of the Mexican corn root; the variety of corn "Herculex RW" (Dow, Pioneer) with tolerance to glufosinate and resistance to lepidoptera (Cry34 / 35Ab1), that is, against the rootworm of western corn, rootworm of northern corn and worm of the root of Mexican corn; the corn variety "Yield Gard VT Rootworm / RR2" with tolerance to glyphosate and resistance to the rootworm of corn; the soybean variety "Optimum GAT" (DuPont, Pioneer) with tolerance to glyphosate and tolerance to the ALS herbicide; the corn variety "Mavera high-value corn" with tolerance to glyphosate, resistance to corn rootworm and European corn borer and high lysine trait.
Examples of commercially available transgenic plants with three traits are the "Herculex I / Roundup Ready 2" maize variety with tolerance to glyphosate, tolerance to glufosinate and lepidopteran resistance (CryI F), ie against the bean cutworm western, corn borer, black cutworm and fall armyworm; the variety of corn "YieldGard Plus / Roundup Ready 2" (Monsanto) with glyphosate tolerance, resistance to corn rootworm and resistance to corn borer; the corn variety "Agrisure GT / CB / LL" (Syngenta) with tolerance to glyphosate, tolerance to glufosinate and resistance to corn borer; the variety of corn "Herculex Xtra" (Dow, Pioneer) with tolerance to glufosinate and resistance to lepidoptera (CryI F + Cry34 / 35Ab1), that is to say, to the worm of the root of the western corn, worm of the root of the corn of the north , Mexican corn rootworm, western bean cutworm, corn borer, black cutworm and fall armyworm; corn varieties "Agrisure CB / LL / RW" (Syngenta) with tolerance to glufosinate, resistance to corn borer (CryIAb) and resistance to lepidoptera (Cry3A), that is, to the rootworm of western corn, worm of the root of northern corn and rootworm of Mexican corn; the corn variety "Agrisure 3000GT" (Syngenta) with tolerance to glyphosate + corn borer resistance (CryIAb) and resistance to lepidoptera (Cry3A), that is, to the western corn rootworm, corn rootworm of the North and worm of the root of the Mexican corn. The methods for producing such transgenic plants are generally known to the person skilled in the art.
An example of a commercially available transgenic plant with four traits is "Hercules Quad-Stack" with tolerance to glyphosate, tolerance to glufosinate, resistance to corn borer and resistance to corn rootworm.
In one embodiment of the invention, the cultivated plant is selected from the group of plants mentioned in the paragraphs and tables of the present description, preferably as mentioned above.
Preferably, the cultivated plants are plants comprising at least one selected trait of herbicide tolerance, insect resistance, for example, by the expression of one or more bacterial toxins, fungal resistance or viral resistance or bacterial resistance by the expression of one or more more antipathogenic substances, tolerance to stress, absorption of nutrients, efficiency in the use of nutrients, modification of the content of chemical products present in the cultivated plant, in comparison with the corresponding control plant.
More preferably, the cultivated plants are plants comprising at least one selected trait of herbicide tolerance, insect resistance by the expression of one or more bacterial toxins, fungal resistance or viral resistance or bacterial resistance by the expression of one or more substances antipathogenic, tolerance to stress, modification of the content of one or more chemical products present in the cultivated plant, in comparison with the corresponding control plant.
Most preferably, the cultivated plants are plants that are tolerant to the action of herbicides and plants that express one or more bacterial toxins, which provides resistance to one or more pests of animals (such as insects or arachnids or nematodes), where the bacterial toxin is preferably a Bacillus thuriginensis toxin. Here, the cultivated plant is preferably selected from soy, corn, rice, cotton, sugar cane, alfalfa, potato, oilseed rape, tomato and cereals such as wheat, barley, rye and oats, most preferably soy, corn , cotton, rice and cereals such as wheat, barley, rye and oats.
Cultivated plants that are tolerant to the action of the herbicides are most preferred.
Most preferably, the plants grown are the plants indicated in Table A. Sources: AgBios database and GMO-compass database (AG BIOS, PO Box 475, 106 St. John St. Merickville, Ontario KOG1 NO, Canada , access: http://www.agbios.com/dbase.php, see also BioTechniques, Volume 35, No. 3, Sept. 2008, page 213, and http://www.gmo-compass.org/eng / gmo / db /).
Table A Most preferably, the cultivated plants are plants that comprise one or more genes as indicated in Table B. Sources: AgBios Database (AG BIOS, PO Box 475, 106 St. John St. Merickville, Ontario KOG1 NO, Canada , access: http://www.agbios.com/dbase.php) Table B No. Gen Cultivation B-1 alfalfa (Medicago sativa) CP4 epsps Preferably, the cultivated plants are plants comprising at least one trait selected from tolerance to herbicides, resistance to insects through the expression of bacterial toxins, fungal resistance or viral resistance or bacterial resistance through the expression of antipathogenic substances, stress tolerance modification of the content of chemical products present in the cultivated plant in comparison with the corresponding wild-type plant.
More preferably, the cultivated plants are plants comprising at least one trait selected from tolerance to herbicides, resistance to insects through the expression of bacterial toxins, fungal resistance or viral resistance or bacterial resistance through the expression of antipathogenic substances, modification of the content of chemical products present in the cultivated plant in comparison with the corresponding wild-type plant.
Most preferably, the cultivated plants are plants tolerant to the action of the herbicides and plants that express bacterial toxins that provide resistance against the pests of animals (such as insects or arachnids or nematodes), where the bacterial toxin is preferably a toxin of Bacillus thuriginensis. At present, the plant is preferably selected from cereals (wheat, barley, rye, oats), soybeans, rice, vine and fruits and vegetables such as tomato, potato and pomo fruits, most preferably soybeans and cereals such as wheat, barley, rye and oats.
Thus, in a preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or its growth locus with a carboxamide compound as defined above, wherein the plant is a plant, which becomes tolerant to herbicides, more preferably to herbicides such as glutamine synthetase inhibitors, 5-enol-pyrovil-shiquimato- inhibitors 3-phosphate synthase, acetolactate synthase inhibitors (ALS), protoporphyrinogen oxidase (PPO) inhibitors, auxin type herbicides, most preferably herbicides such as glyphosate, glufosinate, imazapyr, imazapic, imazamox, imazetapyr, imazaquin, imazametabenz methyl, dicamba and 2,4-D.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopirad, where the plant corresponds to a row of table 1.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound as defined above, preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, penflufen, fluopiram sedaxano, pentiopirad carboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxane and pentiopyrad, where the plant corresponds to a row of table 1.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole -4-carboxamide bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 1.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 1 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-d-fluoro-methyl-1-methyl -1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 1 and the carboxamide compound is pentiopyrad.
Table 1 No Detailed description Plant Literature / commercial plants T1-1 tolerance to imidazolinone cañola B * T1-2 tolerance to corn imidazolinone A *, B * T1-3 tolerance to imidazolinone rice A *, C * T1-4 tolerance to imidazolinone millet A * T1-5 tolerance to imidazolinone barley A * T1-6 tolerance to imidazolinone wheat A * T1-7 tolerance to imidazolinone sorghum A * T1-8 tolerance to imidazolinone oats A * T1-9 tolerance to imidazolinone rye A * T1-10 beet imidazolinone tolerance WO 1998/02526 / WO sugar 1998/02527 T1-1 1 tolerance to imidazolinone lentils US2004 / 0187178 T1-12 tolerance to imidazolinone sunflower B * T1-13 tolerance to imidazolinone wheat D * T1 -14 tolerance to glyphosate alfalfa E *; "Roundup Ready Alfalfa" T1 -15 tolerance to apple glyphosate E * T1-16 tolerance to glyphosate barley E * No Detailed description Plant Literature / commercial plants T1-17 tolerance to glyphosate canola E *; V * T1-18 tolerance to corn glyphosate E *; W * T1-19 tolerance to glyphosate cotton E *; X * T1 -20 tolerance to glyphosate flax E * T1-21 tolerance to glyphosate grape E * T1-22 tolerance to glyphosate lentils E * T1 -23 tolerance to glyphosate rape E * oleaginous T1 -24 tolerance to glyphosate pea E * T1-25 tolerance to glyphosate potato E * T1-26 tolerance to glyphosate rice "Roundup Ready Rice" (Monsanto) T1 -27 tolerance to soy glyphosate E *; Y* T1 -28 tolerance to beet glyphosate E * sugar T1-29 tolerance to sunflower glyphosate E * T1-30 tolerance to glyphosate tobacco E * T1 -31 tolerance to tomato glyphosate E * T1 -32 tolerance to grass glyphosate E * T1 -33 tolerance to glyphosate wheat E * T1 -34 tolerance to glufosinate cañola F *; OR* T1-35 tolerance to glufosinate corn p *. z * T1-36 tolerance to glufosinate cotton F *; "FiberMax Liberty Link" (Bayer), T1-37 tolerance to glufosinate potato F * T1-38 tolerance to glufosinate rice F *. G *; "Liberty Link Rice" (Bayer), T1-39 glufosinate beet tolerance F * sugar T1-40 glufosinate soya tolerance US 6376754 T1-41 glufosinate tolerance to tobacco F * T1 -42 tolerance to glufosinate tomato F * T1-43 tolerance to dicamba poroto US7105724 T1-44 tolerance to dicamba corn US 7105724, WO 2008/051633 T1-45 tolerance to dicamba cotton US7105724, US5670454 T- 6 tolerance to dicamba pea US7105724 T1-47 tolerance to papa dicamba US7105724 T1-48 tolerance to dicamba sorghum US7105724 T1-49 tolerance to soybean dicamba US7105724, US5670454 T1 -50 tolerance to sunflower dicamba US7105724 T1-51 tolerance to tobacco dicamba US7105724 T1 -52 tolerance to tomato dicamba US7105724, US5670454 T1-53 tolerance to bromoxynil cannon "Navigator", "Compass" (Rhone-Poulenc) T1 -54 tolerance to bromoxynil cotton "BXN" (calgene) No Detailed description Plant Literature / commercial plants Protox (L *) sugar T1 -85 tolerance to wheat inhibitor M * Protox (L *) T1 -86 tolerance to soy imidazolinone N * A * refers to US 4761373, US 5304732, US 5331 107, US 5718079, US 6211438, US 621 1439 and US 6222100.
B * refers to Tan et. al, Pest Manag. Sci 61, 246-257 (2005).
C * refers to rice plants resistant to the imidazolinone herbicide with specific mutation of the acetohydroxy acid synthase gene: S653N (see, for example, US 2003/0217381), S654K (see, for example, US 2003/0217381), A122T (see , for example, WO 2004/106529) S653 (At) N, S654 (At) K, A122 (At) T and other resistant rice plants described in WO 2000/27182, WO 2005/20673 and WO 2001/85970 or patents US 5545822, US 5736629, US 5773703, US 5773704, US-5952553, US 6274796, wherein the plants with S653A and A122T mutation are most preferred. D * refers to WO 2004/106529, WO 2004/16073, WO 2003/14357, WO 2003/13225 and WO 2003/14356.
E * refers to US 5188642, US 4940835, US 5633435, US 5804425 and US 5627061.
F * refers to US 5646024 and US 5561236.
G * refers to US 6333449, US 69331 1 1 and US 6468747.
H * refers to US 6153401, US 6100446, WO 2005/107437, US 5670454 and US 5608147. I * refers to WO 2004/055191, WO 199638567 and US 6791014.
K * refers to HPPD inhibitor herbicides, such as isoxazoles (eg, isoxaflutole), diketonitriles, trikeones (eg, sulcotrione and mesotrione), pyrazinate. L * refers to protoporphyrinogen oxidase (PPO) inhibitory herbicides.
M * refers to US 2002/0073443, US 20080052798, Pest Management Science, 61, 2005, 277-285.
N * refers to herbicide-tolerant soybean plants presented under the name of Cultivance at the 16th Brazilian Sementes Congress, from August 31 to September 3, 2009 at Estagáo Embratel Convention Center - Curitiba / PR, Brazil U * "InVigor" (Bayer) V * "Roundup Ready Cañóla" (Monsanto) W * "Roundup Ready Corn", "Roundup Ready 2" (Monsanto), "Agrisure GT", "Agrisure GT / CB / LL", "Agrisure GT / RW", "Agrisure 3000GT" (Syngenta), "YieldGard VT Rootworm / RR2", "YieldGard Triple VT" (Monsanto) X * "Roundup Ready Cotton", "Roundup Ready Flex" (Monsanto) And * "Roundup Ready Soybean" (Monsanto), "Optimum GAT" (DuPont, Pioneer) Z * "Liberty Link" (Bayer), "Herculex I", "Herculex RW", "Herculex Xtra" (Dow, Pioneer), "Agrisure GT / CB / LL", "Agrisure CB / LL / RW" (Syngenta), A subset of especially preferred herbicide tolerant plants is provided in Table 2. In this subset, there are more preferred embodiments: In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound as defined above, preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, penflufen, fluopiram sedaxan, pentiopyrad carboxin, fenfuram, flutolanil; mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-d-fluoro-methyl-1-methyl-1H-pyrazole -4-carboxamide, penflufen, fluopiram, sedaxane and pentiopyrad, where the plant corresponds to a row of table 2.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole -4-carboxamide bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 2.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their locus. growth with a carboxamide compound, wherein the plant corresponds to a row of table 2 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 2 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 2 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 2 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2- il) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is sopirazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-11, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-11, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is N- ( 3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-1 1, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T2-3, T2-5, T2-10, T2-11, T2-16, T2-17 and T2-23 of Table 2 and the carboxamide compound is pentiopyrad.
Table 2 A * refers to US 4761373, US 5304732, US 5331 107, US 5718079, US 6211438, US 621 1439 and US 6222100.
B * refers to Tan et. al, Pest Manag. Sci 61, 246-257 (2005).
C * refers to rice plants resistant to the imidazolinone herbicide with specific mutation of the acetohydroxy acid synthase gene: S653N (see, for example, US 2003/0217381), S654K (see, for example, US 2003/0217381), A122T (see , for example, WO 04/106529) S653 (At) N, S654 (At) K, A122 (At) T and other resistant rice plants described in WO 2000/27182, WO 2005/20673 and WO 2001/85970 or patents US 5545822, US 5736629, US 5773703, US 5773704, US-5952553, US 6274796, wherein plants with S653A and A122T mutation are most preferred. D * refers to WO 04/106529, WO 04/16073, WO 03/14357, WO 03/13225 and WO 03/14356.
E * refers to US 5188642, US 4940835, US 5633435, US 5804425 and US 5627061.
F * refers to US 5646024 and US 5561236.
G * refers to US 6333449, US 69331 1 1 and US 6468747.
H * refers to US 6153401, US 6100446, WO 2005/107437 and US 5608147.
I * refers to Federal Register (USA), Vol. 61, No.160, 1996, page 42581. Federal Register (USA), Vol. 63, No.204, 1998, page 56603.
N * refers to herbicide tolerant soybean plants presented under the name of Cultivance at the 16th Brazilian Congress of Sementes, from August 31 to September 3, 2009 at Estacao Embratel Convention Center - Curitiba / PR, Brazil U * "Roundup Ready Cañóla" (Monsanto) V * "Roundup Ready Corn", "Roundup Ready 2" (Monsanto), "Agrisure GT", "Agrisure GT / CB / LL", "Agrisure GT / RW", "Agrisure 3000GT" (Syngenta), "YieldGard VT Rootworm / RR2", "YieldGard Triple VT" (Monsanto) W * "Roundup Ready Cotton", "Roundup Ready Flex" (Monsanto) x * "Roundup Ready Soybean" (Monsanto), "Optimum GAT" (DuPont, Pioneer) And * "Liberty Link" (Bayer), "Herculex I", "Herculex RW", "Herculex Xtra" (Dow, Pioneer), "Agrisure GT / CB / LL", "Agrisure CB / LL / RW '(Syngenta) Z * "Navigator", "Compass" (Rhone-Poulenc) In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyramide , sedaxane, isopyrazam and pentiopyrad where the plant is a plant, which expresses at least one insecticidal toxin, preferably a toxin of the species Bacillus, more preferably of Bacillus thuringiensis.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound as defined above, preferably with a carboxamide compound selected from boscalid, N- ( 3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 3.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxin, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole -4-carboxamide bixafen, fluopiram, isopyrazam and pentiopyrad, where the plant corresponds to a row of table 3.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 3 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 3 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant corresponds to a row of Table 3 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 3 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 3 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 3 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 3 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 3 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 3 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a carboxamide compound, wherein the plant corresponds to a row of the Table 3 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 3 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 3 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant is selected from T3-13, T3-1, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of the Table 3 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of the Table 3 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of the Table 3 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of the Table 3 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of the Table 3 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant is selected from T3-13, T3-1, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of the Table 3 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of Table 3 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of Table 3 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of Table 3 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T3-13, T3-14, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of Table 3 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, where the plant is selected from T3-13, T3-1, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of Table 3 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, where the plant is selected from T3-13, T3-1, T3-15, T3-16, T3-17, T3-18, T3-19, T3-20, T3-23 and T3-25 of Table 3 and the carboxamide compound is pentiopyrad.
Table 3 No Detailed description Plant Literature / commercial plants resistance to the worm T3-1 corn B * the root of corn Borer resistance T3-2 corn C * of corn resistance to the worm T3-3 corn bean cutter D * western resistance to the worm T3-4 corn E * black cutter resistance to the worm "Herculex I" (Dow, Pioneer), T3-5 corn autumn buddy "Herculex Xtra" (Dow, Pioneer) T3-6 resistance to cotton worm "Bollgard I" (Monsanto), "Bollgard II" A * refers to "Zhuxian B", WO 2001/021821, Molecular Breeding, Volume 18, Number 1 / August 2006.
B * "YieldGard corn rootworm" (Monsanto), "YieldGard Plus" (Monsanto), "YieldGard VT" (Monsanto), "Herculex RW" (Dow, Pioneer), "Herculex Rootworm" (Dow, Pioneer), "Agrisure 0CRW "(Syngenta) C * "YieldGard corn borer" (Monsanto), "YieldGard Plus" (Monsanto), "YieldGard VT Pro" (Monsanto), "Agrisure CB / LL" (Syngenta), "Agrisure 3000GT" (Syngenta), "Hercules I" , "Hercules II" (Dow, Pioneer), "KnockOut" (Novartis), "NatureGard" (Mycogen), "StarLink" (Aventis) D * "Newl_eaf" (Monsanto), "NewLeaf Y" (Monsanto), "NewLeaf Plus" (Monsanto), US6100456 In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 Hp -razol-4-carboxamide, bixafen, penflufen, fluopyram , sedaxane, isopyrazam and pentiopyrad, wherein the plant is a plant that exhibits greater resistance to fungal, viral and bacterial diseases, more preferably a plant expressing antipathogenic substances, such as antifungal proteins, or having acquired systemic resistance properties.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 4.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treatment of cultivated plants, parts of said plants or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1- methyl-1 H-pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpyr, mepronil, oxycarboxin, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5 ' -trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam and pentiopyrad, where the plant corresponds to a row of table 4.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 4 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 4 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 4 and the carboxamide compound is pentiopyrad.
Table 4 No Detailed description Plant Literature T4-1 Fungal resistance apple A *, B *. C * T4-2 barley fungal resistance A *, B *. C * T4-3 Banana fungal resistance A *, B *. C * T4-4. Fungal resistance poroto B *, C * T4-5 corn fungal resistance A *, B *. C * T4-6 fungal resistance cotton A *, C * T4-7 fungal resistance cucumber B *. C * T4-8. Grape fungal resistance C * T4-9. fungal resistance oats A *, C * T4-10. Mushroom resistance pepper B *. C * T4-1 1. fungal resistance potato A *, B *, C * T4-12. fungal resistance B * rape. C * T4-13. fungal resistance rice A *, B *. C * T4-14. Rye fungal resistance A *, B *, C * T4-15 fungal resistance sorghum B *, C * T4-16 Soy fungal resistance A *, B *. C * T4-17. fungal resistance sugarcane B *, C * T4-18 tobacco fungal resistance A *, B *. C * T4-19. tomato fungal resistance A *, B *, C * T4-20. fungal resistance wheat A *, B *. C * T4-21. bacterial resistance apple D * T4-22. Bacterial resistance barley D * T4-23. Bacterial resistance banana D * T4-24. bacterial resistance poroto D * T4-25. corn bacterial resistance T4-26. Bacterial resistance cotton D * T4-27. bacterial resistance cucumber D * T4-28. Bacterial resistance grape D *, US 6172280 T4-29. Bacterial resistance oats D * T4-30 Bacterial resistance pepper D * No Detailed description Plant Literature T4-31. Bacterial resistance potato D * T4-32. bacterial resistance rape D * T4-33. Bacterial resistance rice D * T4-34. Rye bacterial resistance D * T4-35. Bacterial resistance sorghum D * T4-36. Bacterial resistance soy D * T4-37. Bacterial resistance sugar cane D * T4-38. Bacterial resistance tobacco D * T4-39. bacterial resistance tomato D * T4-40. Bacterial resistance wheat D * T4-41. viral resistance apple C * T4-42. viral resistance barley C * T4-43. viral resistance banana C * T4-44. viral resistance poroto C * T4-45. viral resistance corn C * T4-46. viral resistance cotton C * T4-47. cucumber viral resistance C * T4-48. viral resistance oats C * T4-49. viral resistance pepper C * T4-50. viral resistance potato C * T4-51. viral resistance rape C * T4-52. viral resistance rice c * T4-53. rye viral resistance C * T4-54. Viral resistance sorghum C * T4-55. viral resistance soy c * T4-56. viral resistance sugar cane C * T4-57. viral resistance C * tobacco T4-58. viral resistance tomato C * T4-59. C wheat wheat resistance * T4-60. fungal resistance potato E * A * refers to US 5689046 and US 6020129.
B * refers to US 6706952 and EP 1018553, C * refers to US 6630618.
D * refers to WO 1995/005731 and US 5648599.
E * refers to the variety of the potato plant presented for variety registration before the Community Plant Variety Office (CPVO), 3, Boulevard Maréchal Foch, BP 10121, FR - 49101 Angers Cedex 02, France, and that has the CPVO 20082800 file number In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram , sedaxano, sopirazam and pentiopirad, where the plant is a plant that is listed in table 5.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-Trifluoro-biphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 5.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, sopirazam, pentiopyrad, flutolanil , furametpir, mepronil, oxycarboxin, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H- pyrazole-4-carboxamide bixafen, fluopiram, sopirazam and pentiopyrad, where the plant corresponds to a row of table 5.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant corresponds to a row of table 5 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 5 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-11, T5-13 and T5-1 of Table 5 and the carboxamide compound is boscalid In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the carboxamide compound It's bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the carboxamide compound It is fluopiram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the carboxamide compound It is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the carboxamide compound it's pentiopy.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the compound of carboxamide is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-1 of Table 5 and the compound of carboxamide is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the compound of carboxamide is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the compound of carboxamide is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the compound of carboxamide is sedaxane.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T5-2, T5-5, T5-6, T5-9, T5-10, T5-1 1, T5-13 and T5-14 of Table 5 and the compound of carboxamide is pentiopyrad.
Table 5 A * refers to US 5689046 and US 6020129.
B * refers to US 6706952 and EP 1018553.
C * refers to US 6630618.
D * refers to WO 2006/42145, US 5952485, US 5977434, WO 1999/09151 and WO 1996/22375.
E * refers to the variety of the potato plant presented for variety registration before the Community Plant Variety Office (CPVO), 3, Boulevard Maréchal Foch, BP 10121, FR - 49101 Angers Cedex 02, France, and that has the CPVO 20082800 file number In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxano, isopirazam and pentiopirad, where the plant is a plant that is tolerant to abiotic stress, preferably drought, high salinity, high intensity of light, high UV irradiation, chemical pollution (such as high concentration of heavy metals), low or high temperatures, limited supply of nutrients and population stress, most preferably drought, high salinity, low temperatures and limited nitrogen supply.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxane and pentiopyrad, where the plant corresponds to a row of table 6.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole -4-carboxamide bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 6.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 6 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 6 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 6 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 6 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 6 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 6 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 6 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 6 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 6 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 6 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 6 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 6 and the carboxamide compound is pentiopyrad.
Table 6 No Detailed description Plant Literature T6-1 drought tolerance alfalfa A *, B *, F * T6-2 tolerance to drought barley A *, B *, C * No Detailed description Plant Literature T6-3 tolerance to drought cañola A *, B *, F * T6-4 drought tolerance corn A *, B *, C *. F* T6-5 drought tolerance cotton A *, B *. C *, F * T6-6 tolerance to drought fruits pomo A *, B * T6-7 drought tolerance potato A *, B *, C * T6-8 drought tolerance rapeseed A *, B *. C * T6-9 drought tolerance rice A *, B *, C *, F * T6-10 tolerance to drought soybean A *, B *. F* T6-11 drought tolerance beet A *, B * sugar T6-12 tolerance to drought sugarcane A *, B *, F * T6-13 drought tolerance sunflower A *, B * T6-14 tolerance to drought tomato A *, B *, C * T6-15 tolerance to drought wheat A *, B *, C *, F * T6-16 tolerance to high salinity alfalfa A *, B * T6-17 tolerance to high salinity barley A *, B * T6-18 tolerance to high salinity cañola A *, B * T6-19 tolerance to high salinity corn A *, D * T6-20 tolerance to high salinity cotton A *, D * T6-21 tolerance to high salinity fruits knob A *, D * T6-22 tolerance to high salinity potato A *, D * T6-23 tolerance to high rape seed salinity A *, D * T6-24 tolerance to high salinity rice A *, D *, US7034139, WO 2001/30990 T6-25 tolerance to high soy salinity A *, D * T6-26 tolerance to high salinity beet A *, D * sugar T6-27 tolerance to high salinity sugarcane A *, D * T6-28 tolerance to high sunflower salinity A *, D * T6-29 tolerance to high tomato salinity A *, D * T6-30 tolerance to high salinity wheat A *, D * T6-31 tolerance to low temperature alfalfa A *, E * T6-32 tolerance to low temperature barley A * T6-33 tolerance to low temperature cannon A * T6-34 tolerance to low temperature corn A *, E * T6-35 tolerance to low temperature cotton A *, E * T6-36 tolerance to low temperature fruits knob A T6-37 tolerance to low temperature potato A * T6-38 tolerance to low temperature rapeseed A *, E * T6-39 tolerance to low temperature rice A *, E * T6-40 tolerance to low temperature soybean A *, E * T6-41 tolerance to low temperature beet A * sugar T6-42 tolerance to low temperature sugar cane A * T6-43 tolerance to low temperature sunflower A * T6-44 tolerance to low temperature tomato A * No Detailed description Plant Literature T6-45 tolerance to low temperature wheat A *, E * T6-46 tolerance to low supply of alfalfa A * nitrogen T6-47 tolerance to low supply of barley A * nitrogen T6-48 tolerance to low supply of canola A * nitrogen T6-49 tolerance to low supply of corn A * nitrogen T6-50 tolerance to low cotton supply A * nitrogen T6-51 tolerance to low fruit supply knob A * nitrogen T6-52 tolerance to low potato supply A * nitrogen T6-53 tolerance to low supply of rapeseed A * nitrogen T6-54 tolerance to low rice supply A * nitrogen T6-55 tolerance to low supply of soybean A * nitrogen T6-56 tolerance to low supply of beet A * sugar nitrogen T6-57 tolerance to low supply of sugarcane A * nitrogen T6-58 tolerance to low sunflower supply A * nitrogen T6-59 tolerance to low tomato supply A * nitrogen T6-60 tolerance to low wheat supply A * nitrogen A * refers to WO 2000/04173, WO 2007/131699 and US 2008/0229448.
B * refers to WO 2005/48693.
C * refers to WO 2007/20001.
D * refers to US 7256326.
E * refers to US 4731499.
F * refers to WO 2008/002480.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-d-fluoro-methyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxan, isopyrazam and pentiopyrad, where the plant is a plant that is listed in table 7.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxane and pentiopyrad, where the plant corresponds to a row of table 7.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole -4-carboxamide bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 7.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 7 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant corresponds to a row of the table 7 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 7 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 7 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is N- (3 ', 4', 5'- trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is N- (3 ', 4', 5 ' -trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the compound of carboxamide is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T7-5, T7-6, T7-7, T7-8 and T7-9 of Table 7 and the carboxamide compound is pentiopyrad.
Table 7 No Detailed description Plant Literature T7-1 drought tolerance corn A *, B *, C * T7-2 tolerance to drought cañola A *, B *, C * T7-3 drought tolerance cotton A *, B *, C * T7-4 tolerance to drought seed of A *, B *, C * rape T7-5 tolerance to drought rice A *, B *, C * T7-6 tolerance to drought soybean A *, B * T7-7 drought tolerance wheat A *, B *, C * T7-8 tolerance to high salinity rice A *, D *, US7034139, WO 2001/30990 T7-9 tolerance to high tomato salinity A *, D * T7-10 tolerance to low supply cannon A * of nitrogen T7-11 tolerance to low corn supply A * of nitrogen A * refers to WO 2000/04173, WO 2007/131699 and US 2008/0229448.
B * refers to WO 2005/48693.
C * refers to WO 2007/20001.
D * refers to US 7256326.
E * refers to US 4731499.
In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyramide , sedaxano, isopirazam and pentiopirad, where the plant is a plant that exhibits better maturation, preferably maturation, early ripening and late softening of the fruit.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth to carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxano, isopirazam and pentiopirad, where the plant is a plant, which corresponds to a row of table 8 or 8a.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide sedaxane, pentyopyrocardine, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 8 or 8a.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants or their locus of growth. growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam , pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxin, tifiuzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl -1 H-pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam and pentiopyrad, where the plant corresponds to a row of table 8 or 8a.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant corresponds to a row of the table 8 or 8a and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 8 or 8a and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl- 1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is sedaxane In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 8 or 8a and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is N-IS '^'. S'-trifluorobiphenyl ^ -i -S-difluoromethyl-1-methyl-1 H-pyrazole-4- carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T8-1 of Table 8 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants cultured by the treatment of plant propagation materials, preferably seeds of plants grown from cultures with a carboxamide compound, wherein the plant is T8-1 of Table 8 and the carboxamide compound is pentiopyrad.
Table 8 Table 8a In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxano, isopirazam and pentiopirad, where the plant is a transgenic plant, which has modified content in comparison with the wild-type plants, preferably increase in the content of vitamins, alteration of the oil content, reduction of nicotine, increase or reduction of the content of amino acids, alteration of proteins, modification of starch content, alteration of enzymes, alteration of flavonoid content and reduction of allergens (hypoallergenic plants), with maximum preference increase of vitamin content, alteration of oil content, reduction of nicotine, increase of lysine content, alteration of amylase, alteration of amylopectin.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopiram, sedaxano, isopirazam and pentiopirad, where the plant is a plant, corresponding to a row of table 9.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with carboxamide compound selected from boscalid, N - (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 9.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole -4-carboxamide, bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 9.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 9 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 9 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 9 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 9 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 9 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their locus. growth with a carboxamide compound, wherein the plant corresponds to a row of table 9 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to row T9-48 of Table 9 and the carboxamide compound is selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) - 3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, fluopyram, isopyrazam and pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to row T9-49 of Table 9 and the carboxamide compound is selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) - 3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, fluopyram, isopyrazam and pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 9 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 9 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 9 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a carboxamide compound, wherein the plant corresponds to a row of the Table 9 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 9 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 9 and the carboxamide compound is pentiopyrad.
Table 9 No Detailed description Plant Literature / commercial plants T9-1 increase in tomato content US 6797498 Vitamin A T9-2 increase in rice content "Golden rice".
Vitamin A Science 287, 303-305.
T9-3 increase in the content of canola US 7348167, US 11/170711 Vitamin E (application) T9-4 increase in barley content US 1 1 / 170,711 (application) Vitamin E T9-5 increase in corn content US 1 1 / 170.71 1 (application) Vitamin E T9-6 increase in rice content US 1 1 / 170.71 1 (application) Vitamin E T9-7 increase in rye content US 1 1 / 170,711 (application) Vitamin E T9-8 increase in potato content US 7348167 Vitamin E T9-9 increase in soy content US 7348167 Vitamin E T9-10 sunflower content increase US 7348167 Vitamin E T9-11 increase in wheat content US 1 1 / 170.71 1 (application) Vitamin E T9-12 decrease in tobacco content US 2006/0185684, WO Nicotine 2005/000352, WO 2007/064636 No Detailed description Plant Literature / commercial plants ? 9-1? alteration of corn amylase "AmylaseTM" T9-14 alteration of amylopectin potato US 6784338, WO 1997/044471 T9-i e alteration of amylopectin corn US 20070261136 T9-i e modifying the content of Melon A * bitter oil T9-17 modification of the content of canola US 5850026, US6441278, US oil 5723761 T9-18 modification of the content of catalpa A * oil T9-19 modification of the bulrush content A * oil T9-2Q corn content modification A *, US 2006/0075515, US oil 7294759 T9-21 cotton content modification US 6974898, WO 2001/079499 oil T9-22 modification of grape content A * oil T9-22 modification of the seed content of US 5723761 rapeseed oil T9-24 rice content modification A * oil T9-25 modification of soybean content A *, US 6380462, US 6365802, oil "Vistive II", "Vistsive III" T9-26 modification of the content of safflower US 6084164 oil T9-27 Sunflower content modification A *, US 6084164 oil T9-26 modification of wheat content A * oil T9-2S modification of vernonia content A * oil T9-3C soybean hypoallergenic modification US 6864362 T9-31 increased content of canola Bio / Technology 13, 577-582 lysine (1995) T9-32 increased corn content "Mavera high value corn" lysine T9-32 increase in soy content Bio / Technology 13, 577 - 582 lysine (1995) T9-34 alteration of corn content US 7317146, EP 1105511 starch T9-3E alteration of rice content US 7317146, EP 1105511 starch T9-36 alteration of wheat content EP 1105511 starch T9-37 alteration of barley content EP 1105511 starch T9-36 alteration of rye content EP 1105511 starch No Detailed description Plant Literature / commercial plants T9-3S alteration of oat content EP 1 10551 1 starch T9-4C alfalfa content alteration WO 2000/04175 flavonoid T9-41 alteration of apple content WO 2000/04175 flavonoid T9-42 alteration of bean content WO 2000/04175 flavonoid T9-43 alteration of corn content WO 2000/04175 flavonoid T9-44 alteration of grape content WO 2000/04175 flavonoid T9-45 alteration of pea content WO 2000/04175 flavonoid T9-46 alteration of tomato content WO 2000/04175 flavonoid T9-47 increase in soy content "Mavera high value soybeans" protein T9-4S alteration of amylopectin potato B * T9-4S alteration of potato content C * starch A * refers to US 7294759 and US 7157621.
B * refers to the variety of the potato plant presented for variety registration before the Community Plant Variety Office (CPVO), 3, Boulevard Maréchal Foch, BP 10121, FR - 49101 Angers Cedex 02, France, and that has the CPVO 20031520 file number C * refers to the variety of the potato plant presented for variety registration before the Community Plant Variety Office (CPVO), 3, boulevard Maréchal Foch, BP 10121, FR - 49101 Angers Cedex 02, France, and which has the CPVO file number 20082534 In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopiram, sedaxano, isopirazam and pentiopirad, where the plant is a plant corresponding to a row of table 10.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxane and pentiopyrad, where the plant corresponds to a row of table 10.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole -4-carboxamide, bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 10.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 10 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 10 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant corresponds to a row of Table 10 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 10 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 10 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 10 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 10 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 10 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 10 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a carboxamide compound, wherein the plant corresponds to a row of the Table 10 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 10 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 10 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2- il) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2) -yl) -3-cyfluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention is refers to a method of controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a carboxamide compound, wherein the plant is selected from T10-1 , T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T10-1, T10-2, T10-6 and T10-10 of Table 10 and the carboxamide compound is pentiopyrad.
Table 10 No Detailed description Plant Literature / commercial plants T10-1 tomato content increase US 6797498 of vitamin A T10-2 rice content increase "Golden rice". of vitamin A Science 287, 303-305.
T10-3 content increase cannon US 7348167, US 1 1/17071 1 of vitamin E (application) T10-4 decrease of tobacco US 20060185684, WO Nicotine content 2005/000352, WO 2007/064636 T10-5 altered amylase corn "AmylaseTM" T10-6 alteration of potato US 6784338, WO 1997/044471 amylopectin T10-7 modification of cannon US 5850026, US 6441278, US oil content 5723761 T10-8 modification of rape seed US 5723761 oil content T10-9 modification of the safflower US 6084164 oil content T10-10 modification of soybean A *, US 6380462, US 6365802; oil content "Vistive II", "Vistsive III" T10-1 1 increase in soy content "Mavera high valué soybeans" No Detailed description Plant Literature / commercial protein plants T10-12 corn content increase "Mavera high value com" of lysine A * refers to US 7294759 and US 7157621.
In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxano, isopirazam and pentiopirad, where the plant is a plant that exhibits better utilization of nutrients, preferably absorption, assimilation and metabolism of nitrogen and phosphorus.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopiram, sedaxano, isopirazam and pentiopirad, where the plant is a plant corresponding to a row of table 11.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 11.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'- trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpyr, mepronil, oxycarboxin, trifluzamide, more preferably with a selected carboxamide compound of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, fluopyram, isopyrazam and pentiopyrad, wherein the plant corresponds to a row of table 1 1.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant corresponds to a row of table 11 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 11 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T11 -3 and T11-4 of Table 11 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T1 1 -3 and T1 1-4 from Table 11 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl -1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of table 11 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 from Table 11 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of Table 11 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T11-3 and T11-4 of Table 11 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of Table 11 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of Table 11 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3- difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of Table 11 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of Table 11 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of Table 11 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T1 1-3 and T11-4 of Table 11 and the carboxamide compound is pentiopyrad.
Table 1 1 No Detailed description Plant Literature T11-1 nitrogen utilization (D *) alfalfa A *, B *, F * T11-2 nitrogen utilization (D *) barley A *, B * T11-3 nitrogen utilization (D *) cannon A *, B *, F * T11-4 nitrogen utilization (D *) corn A *, B *, F * T11-5 utilization of nitrogen (D *) cotton B *, F * T11-6 nitrogen utilization (D *) potato B *, E *, F * T11 -7 nitrogen utilization (D *) B seed * rape T11 -8 nitrogen utilization (D *) rice A *, B *, F * T11 -9 nitrogen utilization (D *) soybean A *, B *, F * T11 -10 nitrogen utilization (D *) beet B *, E * sugar T11-11 nitrogen utilization (D *) cane B *, E * sugar T11-12 utilization of nitrogen (D *) sunflower B * T11-13 nitrogen utilization (D *) tobacco E *, F * T11-14 nitrogen utilization (D *) tomato B *, F * T11 -15 nitrogen utilization (D *) wheat A *, B *, F * T11-16 use of phosphorus (D *) alfalfa C * T11 -17 utilization of phosphorus (D *) barley C * T11-18 use of phosphorus (D *) canon C * T11 -19 utilization of phosphorus (D *) corn C * T11-20 phosphorus utilization (D *) C cotton * T11 -21 phosphorus utilization (D *) potato US7417181, C * T11-22 use of phosphorus (D *) seed of C * rape T11-23 use of phosphorus (D *) rice C * T11 -24 utilization of phosphorus (D *) soy C * T11-25 utilization of phosphorus (D *) beet C * sugar T11-26 phosphorus utilization (D *) C cane * sugar T11-27 use of phosphorus (D *) sunflower C * T11-28 utilization of phosphorus (D *) tomato US7417181, C * T11-29 use of phosphorus (D *) wheat C * T11-30 tolerance to low supply cannon G * of nitrogen T11-31 tolerance to low supply corn G * of nitrogen A * refers to US 6084153.
B * refers to US 5955651 and US 6864405.
C * refers to US 10 / 898,322 (application).
D * the term "utilization" refers to a better absorption, assimilation or metabolism of nutrients.
E * refers to WO 1995/00991 1.
F * refers to WO 1997/030163.
G * refers to WO 2000/04173, WO 2007/131699 and US 2008/0229448 In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopyrad, wherein the plant is a plant selected from the group consisting of cotton, trees and fiber plants (e.g., palm trees), preferably a plant of cotton, which produces higher quality fiber, preferably better fiber micronarie, greater strength, better fiber length, better uniformity in length and color of the fibers.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cotton plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus. of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, penflufen , fluopiram, sedaxano, isopirazam and pentiopirad.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cotton plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, penflufen, fluopiram sedaxane, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cotton plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H -pyrazol-4-carboxamide, bixafen, fluopyram, isopyrazam and pentiopyrad.
In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxano, isopirazam and pentiopirad, where the plant is a plant that is male sterile or has another trait mentioned in table 12a.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluoro-phenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, bixafen, penflufen , fluopiram, sedaxano, isopirazam and pentiopirad, where the plant is a plant that is listed in table 12 or 12a.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 12 or 12a.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil , furametpir, mepronil, oxycarboxin, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H- pyrazole-4-carboxamide bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 12 or 12a.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their locus. growth with a carboxamide compound, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is sopirazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl- 1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 12 or 12a and the carboxamide compound is pentiopyrad.
Table 12 A * refers to US 6281348, US 6399856, US 7230168, US 6072102.
B * refers to WO 2001/062889.
C * refers to WO 1996/040949.
Table 12a No Plant Event Company Description T12a-1 Brassica MS1, Aventis CropScience Control system of napus (rod RF1 (formerly male sterility plant, Argentina) => PGS1 Genetic Systems) restoration of fertility and pollination that exhibits tolerance to glufosinate herbicide. The MS lines contained the barnase gene of Bacillus amyloliquefaciens, the RF lines contained the barstar gene from the same bacteria and both lines contained the gene encoding phosphinothricin N-acetyltransferase (PAT) from Streptomyces hygroscopicus.
T12a-2 Brassica MS1, Aventis CropScience Napus control system (canola RF2 (formerly male sterility plant, Argentina) => PGS2 Genetic Systems) restoration of fertility and pollination that exhibits tolerance to the glufosinate herbicide. The MS lines contained the sweetened gene from Bacillus amyloliquefaciens, the RF lines contained the barstar gene from the same bacteria and both lines contained the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces hygroscopicus.
T12a-3 Brassica MS8xRF3 Bayer CropScience Control system of napus (cañola (Aventis sterility of the male, Argentina) CropScience (AgrEvo)) restoration of fertility and pollination that exhibits tolerance to the glufosinate herbicide. The MS lines contained the sweetened gene from Bacillus amyloliquefaciens, the RF lines contained the barstar gene from the same bacteria and both lines contained the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces hygroscopicus.
T12a-4 Brassica PHY14, Aventis CropScience The sterility of the napus (canola PHY35 (formerly Plant male was obtained argentina) Genetic Systems) by insertion of the barnase gene Ribonuclease from Bacillus amyloliquefaciens; the restoration of fertility by inserting the RNase inhibitor barstar; resistance to PPT was obtained by PPT-acetyltransferase (PAT) from Streptomyces hygroscopicus.
T12a-4 Brassica PHY36 Aventis CropScience Sterility napus (cañola (formerly Plant male was obtained argentina) Genetic Systems) by inserting the barnase gene Ribonuclease from Bacillus amyloliquefaciens; the restoration of fertility by inserting the RNase inhibitor barstar; resistance to PPT was obtained by PPT-acetyltransferase (PAT) from Streptomyces hygroscopicus.
In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyramide , sedaxane, isopyrazam and pentiopyrad, where the plant is resistant to antibiotics, more preferably resistant to kanamycin, neomycin and ampicillin, most preferably resistant to kanamycin.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-tnfluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopirad, where the plant is a plant corresponding to a row of table 13.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid. , N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, penflufen, fluopyramide, sedative, pentiopyradcarboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 13.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxin, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole -4-carboxamide, bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 13.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 13 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is N- (3 ', 4', 5'-trifluoro-phenyl-2-yl) -3-difluoromethyl-1-methyl. -1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant corresponds to a row of the table 13 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 13 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 13 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, where the plant is T13-6 of the table 13 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of cultivated plants of cultures with. a carboxamide compound, wherein the plant is T13-6 of Table 13 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl- 1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention is refers to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a carboxamide compound, wherein the plant is T13-6 of the Table 13 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is T13-6 of Table 13 and the carboxamide compound is pentiopyrad.
Table 13 A * refers to Plant Cell Reports, 20, 2001, 610-615. Trends n Plant Science, 11, 2006, 317-319. Plant Molecular Biology, 37, 1998, 287-296. Mol Gen Genet., 257, 1998, 606-13. B * refers to Plant Cell Reports, 6, 1987, 333-336.
In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopyramide , sedaxano, isopirazam and pentiopirad, where the plant has the best quality fiber trait.
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their locus of growth with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, penflufen, fluopiram, sedaxano, isopirazam and pentiopirad, where the plant is a cotton plant that includes the event DP 104 B2RF ("DP 104 B2RF- A new early maturing B2RF variety" presented in 2008 Beltwide Cotton Conferences by Tom R. Speed, Richard Sheetz, Doug Shoemaker, Monsanto / Delta and Pine Land, see http://www.monsanto.com/pdf/beltwide_08/dp104b2rf_doc.pdf.
In another preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of cultivated plants by treating cultivated plants, parts of said plants, plant propagation materials or their growth locus with a carboxamide compound selected from boscalid, N-YS '^'. S'-trifluorobiphenyl ^ -ylJ-S-difluoromethyl-l-methyl-l H -pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam and pentiopyrad, wherein the plant is a transgenic plant, having two accumulated traits, more preferably two or more traits selected from the group consisting of herbicide tolerance, insect resistance, fungal resistance, viral resistance, bacterial resistance, stress tolerance , alteration of the maturation, modification of the content and modification of the absorption of nutrients, with most preference, the combination of tolerance to herbicide and resistance to inse These include tolerance to two herbicides, herbicide tolerance and stress tolerance, herbicide tolerance and content modification, tolerance to two herbicides and insect resistance, herbicide tolerance, insect resistance and stress tolerance, herbicide tolerance, resistance to insects and content modification ..
In a more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds, with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, penflufen, fluopyramide sedaxano, pentiopyrad carboxin, fenfuram, flutolanil; mepronil, oxycarboxin, tifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4 -carboxamide, penflufen, fluopiram, sedaxano and pentiopirad, where the plant corresponds to a row of table 14.
In another more preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of selected carboxamide of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazole-4-carboxamide bixafen, fluopyram, isopyrazam, pentiopyrad, flutolanil, furametpir, mepronil, oxycarboxine, trifluzamide, more preferably with a carboxamide compound selected from boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole -4-carboxamide bixafen, fluopiram, isopirazam and pentiopyrad, where the plant corresponds to a row of table 14.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of Table 14 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant corresponds to a row of table 14 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of Table 14 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H -pyrazol-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is sedative.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant corresponds to a row of table 14 and the carboxamide compound is pentiopyrad.
In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 of the Table 14 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 of the Table 14 and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 of the Table 14 and the carboxamide compound is bixafen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14 -20, T14-21, T14-35, T14-36 and T14-37 of Table 14 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 of the Table 14 and the carboxamide compound is isopyrazam.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 of the Table 14 and the carboxamide compound is pentiopyrad.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 Table 14 and the carboxamide compound is boscalid.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 Table 14 and the carboxamide compound is N- (3 ', 4', 5'-thifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T1 -37 Table 14 and the carboxamide compound is penflufen.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 Table 14 and the carboxamide compound is fluopyram.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T1 -36 and T1 -37 Table 14 and the carboxamide compound is sedaxan.
In another most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating plant propagation materials, preferably seeds of plants grown from cultures with a compound of carboxamide, wherein the plant is selected from T14-1, T14-8, T14-13, T14-18, T14-19, T14-20, T14-21, T14-35, T14-36 and T14-37 Table 14 and the carboxamide compound is pentiopyrad.
Table 14 No Detailed description Plant Literature / commercial plants T14-1 Corn Borer Borer Resistance "YieldGard Roundup + tolerance to glyphosate Ready ", YieldGard Roundup Ready 2"(Monsanto) T14-2 corn borer resistance "Agrisure CB / LL" (Syntenta) + glufosinate tolerance T14-3 glyphosate tolerance + maize resistance "Yield Gard VT to Rootworm / RR2 maize rootworm" T14-4 tolerance to glyphosate + corn resistance "Yield Gard VT Triple" to the corn rootworm / corn borer No Detailed description Plant Literature / commercial plants T14-5 tolerance to glufosinate + corn "Herculex I" resistance to lepidoptera (Cry1 F; resistance to the cutterworm western bean, borer corn, black cutworm, autumn wormworm) T1 -6 tolerance to glyphosate + corn resistance "YieldGard Com to Rootworm / Roundup Ready corn rootworm 2"(Monsanto) T1 -7 tolerance to glyphosate + corn tolerance "Herculex I / Roundup to glufosinate + resistance to Ready 2"; lepidoptera (CryI F; resistance to bean cutter worm western, corn borer, worm black cutter, worm autumn budhead) T1 -8 tolerance to glyphosate + corn resistance "YieldGard Plus / Roundup to corn rootworm + Ready 2" (Monsanto) resistance to corn borer T14-9 tolerance to glufosinate + corn "Agrisure GT / RW" resistance to lepidoptera (Cry3A; (Syngenta) resistance to rootworm of western corn, worm of the Northern corn root, worm of Mexican corn root) T14-10 tolerance to glyphosate + corn tolerance "Agrisure GT / CB / LL" to glufosinate + resistance to (Syngenta) corn borer T14-1 1 tolerance to glufosinate + maize "Herculex RW" (Dow, resistance to Lepidoptera Pioneer) (Cry34 / 35Ab1; resistance to corn rootworm Western, root worm North corn, rootworm of Mexican corn) T14-12 tolerance to glufosinate + corn "Herculex Xtra" (Dow, resistance to lepidoptera (CryI F Pioneer) + Cr 34 / 35Ab1; resistance to corn rootworm Western, root worm North corn, rootworm of Mexican corn, worm western bean cutter, corn borer, worm black cutter, armyworm of autumn) No Detailed description Plant Literature / commercial plants T14-13 tolerance to glyphosate + corn tolerance "Herculex Quad-Stack" glufosinate + resistance to corn borer + resistance to the corn rootworm T1 -14 glyphosate tolerance + maize resistance "Yield Gard VT to Rootworm / RR2 maize rootworm" T14- 5 tolerance to glufosinate + corn "Agrisure CB / LL / RW" resistance to corn borer (Syngenta) (CryIAb) + resistance to Lepidoptera (Cry3A; resistance) to the corn rootworm Western, root worm North corn, rootworm of Mexican corn) T14-16 tolerance to glyphosate + corn resistance "Agrisure 3000GT" to corn borer (CryIAb) + (Syngenta) resistance to lepidoptera (Cry3A; resistance to rootworm of western corn, worm of the Northern corn root, worm of Mexican corn root) T14-17 tolerance to glyphosate + corn resistance "Mavera high-value corn" to corn borer and to (Monsanto) corn root worm + high lysine content T14-18 tolerance to glyphosate + soy tolerance "Optimum GAT" (DuPont, to the herbicide ALS (F *) Pioneer) T14-19 resistance to glyphosate + soybean A *, US7432421 resistance to lepidoptera (Bt) T1 -20 glyphosate tolerance + soy tolerance A *, US7105724 to Oicamba T1 -21 tolerance to glyphosate + soy A *, G * modification of the content of oil T14-22 tolerance to glufosinate + soy G *, G modification of the content of oil T14-23 tolerance to glyphosate + tolerance cotton A *, US7105724, to dicamba WO2008051633 T14-24 tolerance to glufosinate + cotton D *, US5646024, tolerance to lepidoptera US5561236 T14-25 tolerance to glyphosate + tolerance cotton A *, D * to lepidoptera A * refers to US 5188642, US 4940835, US 5633435, US 5804425 and US 5627061.
B * refers to rice plants resistant to the imidazolinone herbicide with specific mutation of the acetohydroxy acid synthase gene: S653N (see, for example, US 2003/0217381), S654K (see, for example, US 2003/0217381), A122T (see , for example, WO 2004/106529) S653 (At) N, S654 (At) K, A122 (At) T and other resistant rice plants described in WO 2000/27182, WO 2005/20673 and WO 2001/85970 or patents US 5545822, US 5736629, US 5773703, US 5773704, US-5952553, US 6274796, wherein the plants with S653A and A122T mutation are most preferred. C * refers to WO 2000/04173, WO 2007/131699, US 20080229448 and WO 2005/48693. D * refers to WO 1993/07278 and WO 1995/34656.
E * refers to WO 1996/26639, US 7329802, US 6472588 and WO 2001/17333.
F * refers to sulfonylurea and imidazolinone herbicides, such as imazamox, imazetapyr, imazaquin, chlorimuron, flumetsulam, cloransulam, diclosulam and tifensulfuron. G * refers to US 6380462, US 6365802, US 7294759 and US 7157621.
H * refers to Plant Cell Reports, 20, 2001, 610-615. Trends in Plant Science, 11, 2006, 317-319. Plant Molecular Biology, 37, 1998, 287-296. Mol Gen Genet., 257, 1998, 606-13.
Federal Register (USA), Vol.60, No.113, 1995, page 31 139. Federal Register (USA), Vol.67, No.226, 2002, page 70392. Federal Register (USA), Vol.63, No.88, 1998, page 25194. Federal Register (USA), Vol.60, No.141, 1995, page 37870 Canadian Food Inspection Agency, FD / OFB-095-264-A, October 1999, FD / OFB-099-127-A, October 1999.
I * refers to Federal Register (USA), Vol. 61, No.160, 1996, page 42581. Federal Register (USA), Vol. 63, No.204, 1998, page 56603.
Preferred embodiments of the invention are methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant it is a transgenic plant that is selected from the plants listed in table A.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in table A and the carboxamide compound is boscalid.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table A and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table A and the carboxamide compound is bixafen.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table A and the carboxamide compound is penflufen.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table A and the carboxamide compound is fluopyram.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table A and the carboxamide compound is sedative.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, where the plant is selected from the plants listed in Table A and the carboxamide compound is isopyrazam.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table A and the carboxamide compound is pentiopyrad.
Other preferred embodiments of the invention are methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is a transgenic plant that is selected from the plants listed in table B.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is boscalid.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carboxamide.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is bixafen.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is penflufen.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is fluopyram.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is sedative.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is isopyrazam.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table B and the carboxamide compound is pentiopyrad.
In another preferred embodiment, the present invention relates to methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with an inhibitor of complex III of respiration, where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30 , B-39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B -71 of table B.
In a most preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound. , where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-11, B-23, B-28.B-29, B-30, B- 39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 Table B and the carboxamide compound is boscalid.
In a most preferred embodiment, the present invention is refers to methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from B-3, B -4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30, B-39, B-42, B-44, B- 46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 of Table B and the carboxamide compound is N- ( 3 ', 4', 5'-trifluorobiphenl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide.
In a most preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound. , where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30, B -39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 of Table B and the carboxamide compound is bixafen.
In a most preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound. , where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30, B -39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 of Table B and the carboxamide compound is penflufen.
In a most preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound. , where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30, B -39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 of Table B and the carboxamide compound is fluopyram.
In a most preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound. , where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30, B -39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 of Table B and the carboxamide compound is sedaxane In a most preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound. , where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30, B -39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 of Table B and the carboxamide compound is isopyrazam.
In a most preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound. , where the plant is selected from B-3, B-4, B-5, B-7, B-8, B-1 1, B-23, B-28.B-29, B-30, B -39, B-42, B-44, B-46, B-47, B-55, B-59, B-61, B-63, B-64, B-69, B-70, B-71 of Table B and the carboxamide compound is pentiopyrad.
Other preferred embodiments of the invention are methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CryIAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab , Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG , pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.g.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is boscalid and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CryIAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab, Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, .CrylAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab, Cry34Ab1, Cry35Ab1 , Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG, pinll, PMI , prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is bixafen and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CrylAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab, Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is penflufen and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CrylAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab, Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
In a more preferred embodiment, the present invention relates to methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is fluopyram and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CryIAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab, Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad.2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is sedative and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CryIAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab, Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is isopyrazam and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CryIAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2, Cry2Ab, Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat, PG, pinll, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is pentiopyrad and the plant expresses one or more genes selected from aad, ACCase, ALS, AMY797E, APH4, bar, barnase, barstar, blah, bxn, cDHDPS, CP, cmv-cp, CryIAb, CryIAc, Cry1A.105, CryI F, Cry1 Fa2 , Cry2Ab, Cry34Ab1, Cry35Ab1, Cry3A, Cry3Bb1, Cry9C, dam, DHFR, fad2, fan1, FH, flcrylAb, GAT4601, GAT4602, gmFAD2-1, GM-HRA, goxv247, gus, hel, mCry3A, us, NPTII, pat , PG, pin, PMI, prsv-cp, QTPASE, rep, SAMase, spc, TE, vip3A, vip3A (a), wmv2-cp and zymv-cp.
Other preferred embodiments of the invention are methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryI F, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is boscalid and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryI F, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryI F, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the carboxamide compound is bixafen and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, Cry1 F, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the carbode compound is penflufen and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryI F, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the carbode compound is fluopyram and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryI F, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the carbode compound is sedative and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryI F, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the carbode compound is isopyrazam and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryIF, Cry34Ab1 and Cry35Ab1.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the carbode compound is pentiopyrad and the plant expresses one or more genes selected from CP4 epsps, pat, bar, CryIAb, CryIAc, Cry3Bb1, Cry2Ab, CryI F, Cry34Ab1 and Cry35Ab1.
Other preferred embodiments of the invention are methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the plant is a transgenic plant that is selected from the plants listed in table C.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the plant is selected from the plants listed in Table C and the carbode compound is boscalid.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the plant is selected from the plants listed in Table C and the carbode compound is N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H- pyrazole-4-carbode.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carbode compound, wherein the plant is selected from the plants listed in Table C and the carbode compound is bixafen.
In a more preferred embodiment, the present invention relates to methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table C and the carboxamide compound is penflufen.
In a more preferred embodiment, the present invention relates to methods of controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table C and the carboxamide compound is fluopyram.
In a more preferred embodiment, the present invention relates to methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table C and the carboxamide compound is sedative.
In a more preferred embodiment, the present invention relates to methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table C and the carboxamide compound is isopyrazam.
In a more preferred embodiment, the present invention relates to methods for controlling harmful fungi and / or increasing the health of the plants by treating cultivated plants, parts of said plants or their growth locus with a carboxamide compound, wherein the plant is selected from the plants listed in Table C and the carboxamide compound is pentiopyrad.
Table C (source: Phillips McDougall AgriService, Seed Service May 2009) No Name of the seed Cultivation Company C-1 Agrisure 3000GT Corn Syngenta C-2 Agrisure CB / LL Corn Syngenta C-3 Agrisure CB / LLJRW Corn Syngenta C-4 Agrisure GT corn Syngenta C-5 Agrisure GT / CB / LL Syngenta corn C-6 Agrisure GT / RW Corn Syngenta C-7 Agrisure RW Corn Syngenta C-8 Bollgard cotton Monsanto C-9 Bollgard II cotton Monsanto C-10 Bollgard II RR Flex Cotton Cotton Monsanto C-11 Bt-Xtra DeKalb corn C-12 Clearfield Canoe Canoe BASF C-13 Clearfield with BASF corn C-14 Clearfield rice rice BASF C-15 Clearfield sunflower sunflower BASF C-16 Clearfield wheat wheat BASF C-17 Herculex 1 Dow / Pioneer corn C-18 Herculex Quad-Stack Corn Dow / Pioneer C-19 Herculex RW Corn Dow / Pioneer C-20 Herculex XTRA Dow / Pioneer corn C-21 Herculex Xtra Corn Dow / Pioneer C-22 Knock Out Corn Novartis C-23 Liberty Link cañadala AgrEvo C-24 Liberty Link corn Bayer C-25 Liberty Link cotton Bayer C-26 Maize Maximiser Syngenta C-27 Nature Guard® Dow Corn C-28 New Leaf Potato potato Monsanto C-29 Optimum GAT DuPont corn C-30 Optimum GAT DuPont cotton C-31 Optimum GAT soy DuPont C-32 Poet Compatibel Corn BASF C-33 Roundup Ready 2 Yield cañóla Monsanto C-34 Roundup Ready 2 Yield Monsanto corn C-35 Roundup Ready 2 Yield Cotton Monsanto C-36 Roundup Ready 2 Yield Soy Monsanto C-37 Roundup Ready Alfalfa alfalfa Monsanto C-38 Roundup Ready Bollgard Cotton Monsanto C-39 Roundup Ready Bollgard II Cotton Monsanto C-40 Roundup Ready Cañóla cañóla Monsanto C-41 Roundup Ready Corn Corn Monsanto C-42 Roundup Ready Corn Corn 2 Monsanto C-43 Roundup Ready Cotton Cotton Monsanto C-44 Roundup Ready Flex Cotton Monsanto C-45 Roundup Ready Flex Bollgard II Cotton Monsanto C-46 Roundup Ready Soybean Soy Monsanto C-47 Roundup Ready Sugarbeet remolach KWS / SES / Hilleshog a sugar C-48 Roundup Ready YieldGard corn borer Monsanto C-49 Roundup Ready YieldGard Plus Corn Monsanto C-50 Roundup Ready, Herculex XTRA Dow / Pioneer corn C-51 StarLink Corn Aventis C-52 Widestrike cotton Dow C-53 YieldGard corn Monsanto C-54 YieldGard corn borer and corn corn Monsanto rootworm C-55 YieldGard Corn Rootworm Corn Monsanto C-56 YieldGard Plus RR Corn 2 Corn Monsanto C-57 YieldGard rootworm RR Corn 2 corn Monsanto C-58 YieldGard corn Monsanto In a most preferred embodiment, the present invention relates to a method for controlling harmful fungi and / or increasing the health of the cultivated plants by treating cultivated plants, parts of said plants or their growth locus with a compound of carboxamide, wherein the plant and the carboxamide compound are selected as indicated in Table D.
Table D No Pesticide Cultivation Gen D-1 Boscalid cañola bar D-2 Boscalid cañóla bxn D-3 Boscalid cañola CP4 epsps D-4 Boscalid cañóla goxv247 D-5 Boscalid cañola pat D-6 Boscalid corn CP4 epsps D-7 Boscalid Corn CryIAb D-8 Boscalid corn CryIAc D-9 Boscalid Corn CryIF D-10 Boscalid corn Cry1Fa2 D-11 Boscalid corn Cry34Ab1 D-12 Boscalid corn Cry35Ab1 D-13 Boscalid corn Cry3A D-14 Boscalid corn Cry3Bb1 D-15 Boscalid corn Cry9C D-16 Boscalid corn goxv247 D-17 Boscalid corn pat D-18 Boscalid corn vip3A D-19 Boscalid cotton ALS D-20 Boscalid cotton bxn D-21 Boscalid cotton CP4 epsps D-22 Boscalid CryIAc cotton D-23 Boscalid cotton CryIF D-24 Boscalid cotton Cry2Ab D-25 Boscalid cotton pat D-26 Boscalid cotton vip3A (a) D-27 Boscalid soy ALS D-28 Boscalid soya CP4 epsps D-29 Boscalid soybean pat D-30 N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1- barrel bar methyl-1 H-pyrazole-4-carboxamide D-31 N-YS '^'. S'-trifluorobiphenyl ^ -i -S-difluoromethyl-l- cañóla bxn methyl-1 H-pyrazole-4-carboxamide D-32 N- (3,, 4 ', 5, -trifluorobiphenyl-2-yl) -3-difluoromethyl-1- barrel CP4 methyl-1 H-pyrazole-4-carboxamide epsps D-33 N- (3,, 4 ', 5, -trifluorobiphenyl-2-yl) -3-d-fluoro-methyl-1- cañola goxv247 methyl-1 H-pyrazole-4-carboxamide D-34 N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1- barrel pat methyl-1 H-pyrazole-4-carboxamide D-56 N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-soybean ALS methyl-1 H-pyrazole-4-carboxamide D-57 N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-soybean CP4 methyl-1 H-pyrazole-4-carboxamide epsps D-58 N-CS '^'. S'-trifluorobiphenyl ^ -i -S-difluoromethyl-l-soybean pat methyl-1 H-pyrazole-4-carboxamide All embodiments of the carboxamide compound as defined above are also referred to in the present carboxamide compound according to the present invention. They can also be converted into agrochemical compositions comprising a solvent or a solid carrier and at least one carboxamide compound according to the present invention.
An agrochemical composition comprises an effective amount as a fungicide and / or effective for the health of the plant of a carboxamide compound according to the present invention. The term "effective amount" indicates an amount of the carboxamide composition or compounds according to the present invention, which is sufficient to achieve the synergistic effects related to fungal control and / or health of the plant, and which does not causes substantial damage to the treated plants. Said quantity can vary in a wide range and depends on several factors such as the fungal species to be controlled, the material or the cultivated plant treated, the climatic conditions.
Examples of agrochemical compositions are solutions, emulsions, suspensions, powders, powders, pastes and granules. The type of composition depends on the particular purpose intended; in each case, it should be ensured that the distribution of the compound according to the invention is fine and uniform.
More precise examples of the types of compositions are suspensions (SC, OD, FS), pastes, pellets, powders or wettable powders (WP, SP, SS, WS, DP, DS) or granules (GR, FG, GG, MG), which can be water-soluble or wettable, as well as gel formulations for the treatment of plant propagation materials, such as seeds (GF). Usually, the types of compositions (for example, SC, OD, FS, WG, SG, WP, SP, SS, WS, GF) are used diluted. Usually, the types of compositions such as DP, DS, GR, FG, GG and MG are used undiluted.
The compositions are prepared in the known manner (cf. US 3,060,084, EP-A 707 445 (for liquid concentrates), Browning: "Agglomeration", Chemical Engineering, Dec. 4, 1967, 147-48, Perry's Chemical Engineer's Handbook , 4th Ed., McGraw-Hill, New York, 1963, S. 8-57 und ff, WO 91/13546, US 4,172,714, US 4,144,050, US 3,920,442, US 5,180,587, US 5,232. 701, US 5,208,030, GB 2,095,558, US 3,299,566, Klingman: Weed Control as a Science (J. Wiley &Sons, New York, 1961), Hance et al .: Weed Control Handbook (8th Ed. , Blackwell Scientific, Oxford, 1989) and Mollet, H. and Grubemann, A .: Formulation technology (Wiley VCH Verlag, Weinheim, 2001).
The agrochemical compositions may also comprise auxiliaries that are customary in agrochemical compositions. The auxiliaries used depend on the form of application and the particular active substance, respectively.
Examples of suitable auxiliaries are solvents, solid carriers, dispersants or emulsifiers (such as other solubilizers, protective colloids, surfactants and adhesion agents), organic and anorganic thickeners, bactericides, antifreeze agents, antifoaming agents, if suitable dyes and builders. stickiness or binders (for example, for formulations for seed treatment).
Suitable solvents are water, organic solvents such as fractions of mineral oil whose boiling point is medium to high, such as kerosene or gas oil, as well as coal tar oils and oils of vegetable or animal origin, aliphatic, cyclic hydrocarbons and aromatics, for example toluene, xylene, paraffin, tetrahydronaphthalene, alkylated naphthalenes or their derivatives, alcohols such as methanol, ethanol, propanol, butanol and cyclohexanol, glycols, ketones such as cyclohexanone and gamma-butyrolactone, fatty acid dimethylamides, fatty acids and esters of fatty acids and highly polar solvents, for example amines such as N-methylpyrrolidone.
Solid carriers are mineral soils such as silicates, silica gels, talc, kaolin, limestone, quicklime, chalk, bolus, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, magnesium oxide, ground synthetic materials , fertilizers, such as, for example, ammonium sulfate, ammonium phosphate, ammonium nitrate, ureas, and products of vegetable origin, such as cereal flour, tree bark flour, wood flour and nut shell flour, cellulose powders and other solid carriers.
Suitable surfactants (adjuvants, humectants, tackifiers, dispersants or emulsifiers) are alkali metal, alkaline earth metal and ammonium salts of aromatic sulfonic acids, such as ligninsulfonic acid (Borresperse® types, Borregard, Norway), phenolsulfonic acid, Naphthalenesulfonic acid (Morwet® types, Akzo Nobel, USA), dibutylnaphthalene sulphonic acid (Nekal® types, BASF, Germany) and fatty acids, alkylsulfonates, alkylarylsulfonates, alkyl sulfates, lauryl ether sulphates, fatty alcohol sulphates, and hexa-, hepta- and sulphated octadecanolates, glycol ethers of sulfated fatty alcohols, in addition to condensates of naphthalene or naphthalenesulfonic acid with phenol and formaldehyde, polyoxyethylene octyphenyl ether, sodium methoxy phenyl ethoxylated, octylphenol, nonylphenol, alkylphenyl polyglycol ethers, tributylphenyl polyglycol ether, tristearylphenyl polyglycol ether, alkylaryl polyester alcohols, ethylene oxide / alcohol and fatty alcohol condensates, ethoxylated castor oil, polyoxyethylene alkyl ethers, ethoxylated polyoxypropylene, lauryl alcohol polyglycol ether acetal, sorbitol esters, residual liquors of lignin-sulfite and proteins, denatured proteins, polysaccharides (for example, methylcellulose), hydrophobically modified starches, polyvinyl alcohols (Mowiol® types, Clariant, Switzerland), polycarboxylates (Sokolan® types, BASF, Germany), polyalkoxylates, polyvinylamines (Lupasol® types, BASF, Germany), polyvinylpyrrolidone and its copolymers.
Examples of thickeners (i.e. compounds which impart a capacity modified flow compositions, ie high viscosity under static and low viscosity conditions during agitation) are polysaccharides and inorganic and organic clays such as xanthan gum (Kelzan, CP Kelco, USA), Rhodopol® 23 (Rhodia, France), Veegum® (RT Vanderbilt, USA) or Attaclay® (Engelhard Corp., NJ, United States).
Bactericides can be added for the preservation and stabilization of the composition. Examples of suitable bactericides are those based on dichlorophen and hemi formal benzylalcohol (Proxel® from ICI or Acticide® RS from Thor Chemie and Kathon® MK from Rohm &Haas) and isothiazolinone derivatives such as alkylisothiazolinones and benzisothiazolinones (Acticide® MBS of Thor Chemie).
Examples of suitable antifreeze agents are ethylene glycol, propylene glycol, urea and glycerin.
Examples of antifoams are silicone emulsions (such as, for example Silikon SRE, Wacker, Germany or Rhodorsil, Rhodia, France), alcohole long chain fatty acids, fatty acid salts, fluoro-compounds and mixtures thereof.
Suitable dyes are pigments of low water solubility and water-soluble dyes. The examples that can be mentioned are the rhodamin B designations, CI pigment red 112, CI solvent red 1, pigment blue 15: 4, pigment blue 15: 3, pigment blue 15: 2, pigment blue 15: 1, pigment blue 80, pigment yellow 1, pigment yellow 13, pigment red 112, red pigment 48: 2, red pigment 48: 1, red pigment 57: 1, red pigment 53: 1, orange pigment 43, orange pigment 34, orange pigment 5, green pigment 36, green pigment 7, white pigment 6, brown pigment 25, basic violet 10, basic violet 49, acid red 51, acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108.
Examples of tackifiers or binders are polyvinylpyrrolidones, polyvinylacetates, polyvinyl alcohols and cellulose ethers (Tylose®, Shin-Etsu, Japan).
The powders, spreading materials and powders can be prepared by mixing or commingling the compounds I and, if appropriate, other active substances, with at least one solid carrier.
The granules, for example coated granules, impregnated granules and homogeneous granules, can be prepared by joining the active substances with solid carriers. Examples of solid carriers are mineral soils such as silica gels, silicates, talc, kaolin, attaclay, limestone, quick lime, chalk, bolus, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, magnesium oxide , ground synthetic materials, fertilizers, such as, for example, ammonium sulfate, ammonium phosphate, ammonium nitrate, ureas, and products of vegetable origin, such as cereal flour, tree bark flour, wood flour and flour. nut shell, cellulose powder and other solid carriers.
Examples of types of compositions are: 1. . Types of compositions to be diluted with water i) Water-soluble concentrates (SL, LS) 10 parts by weight of a carboxamide compound according to the present invention are dissolved in 90 parts by weight of water or in a water-soluble solvent. As an alternative, wetting agents or other auxiliaries are added. The active substance dissolves when diluted with water. In this way, a composition having a content of 10% by weight of active substance is obtained, ii) Dispersible concentrates (DC) 20 parts by weight of a carboxamide compound according to the present invention are dissolved in 70 parts by weight of cyclohexanone with addition of 10 parts by weight. weight of a dispersant, for example polyvinylpyrrolidone. Dilution with water produces a dispersion. The content of active substance is 20% by weight. iii) Emulsifiable concentrates (EC) 15 parts by weight of a carboxamide compound according to the present invention are dissolved in 75 parts by weight of xylene with addition of calcium dodecylbenzenesulfonate and ethoxylated castor oil (in each case, 5 parts by weight). Dilution with water produces an emulsion. The composition has an active substance content of 15% by weight. iv) Emulsions (EW, EO, ES) 25 parts by weight of a carboxamide compound according to the present invention are dissolved in 35 parts by weight of xylene with addition of calcium dodecylbenzenesulfonate and ethoxylated castor oil (in each case, 5 parts by weight). This mixture is introduced in 30 parts by weight by an emulsifying machine (Ultraturrax) and converted into a homogeneous emulsion. Dilution with water produces an emulsion. The composition has an active substance content of 25% by weight. v) Suspensions (SC, OD, FS) In a shaking ball mill, 20 parts by weight of a carboxamide compound according to the present invention are comminuted with addition of 10 parts by weight of dispersants and wetting agents and 70 parts by weight of water or an organic solvent to produce a fine suspension of the active substance. Dilution with water produces a stable suspension of the active substance. The content of active substance in the composition is 20% by weight. vi) Granules dispersible in water and water soluble granules (WG, SG) 50 parts by weight of a carboxamide compound according to the present invention are finely milled with the addition of 50 parts by weight of dispersants and wetting agents and prepared as water-dispersible or water-soluble granules by means of technical devices (eg, extrusion , spray tower, fluidized bed). Dilution with water produces a stable dispersion or solution of the active substance. The composition has an active substance content of 50% by weight. vii) Dispersible powders in water and water soluble powders (WP, SP, SS, WS) 75 parts by weight of a carboxamide compound according to the present invention are ground in a rotor-stator mill with addition of 25 parts by weight of dispersants, wetting agents and silicagel. Dilution with water produces a stable dispersion or solution of the active substance. The content of active substance of the composition is 75% by weight. viii) Gel (GF) In a stirred ball mill, 20 parts by weight of a carboxamide compound according to the present invention are comminuted with addition of 10 parts by weight of dispersants, 1 part by weight of gelling agent humectants and 70 parts by weight of water or an organic solvent to produce a fine suspension of the active substance. Dilution with water produces a stable suspension of the active substance, by which a composition with 20% (w / w) of active substance is obtained. 2. Types of compositions to be applied undiluted ix) Powders that can be converted into powders (DP, DS) 5 parts by weight of a carboxamide compound according to the present invention are finely ground and intimately mixed with 95 parts by weight of finely divided kaolin. This produces a composition which can be converted to powder and which has an active substance content of 5% by weight. x) Granules (GR, FG, GG, MG) 0.5 parts by weight of a carboxamide compound according to the present invention are finely ground and associated with 99.5 parts by weight of carriers. Current methods are extrusion, spray drying or fluidized bed. This produces granules to be applied undiluted and having an active substance content of 0.5% by weight. xi) ULV Solutions (UL) 10 parts by weight of a carboxamide compound according to the invention are dissolved in 90 parts by weight of an organic solvent, for example xylene. This produces a composition to be applied undiluted and having an active substance content of 10% by weight.
The agrochemical compositions generally comprise between 0.01 and 95%, preferably between 0.1 and 90%, most preferably between 0.5 and 90%, by weight of active substance. The active substances are used in a purity of 90% to 100%, preferably 95% to 100% (according to the NMR spectrum).
Water-soluble concentrates (LS), fluidizable concentrates (FS), powders for dry treatment (DS), water-dispersible powders for suspension treatment (WS), water-soluble powders (SS), emulsions (ES), emulsifiable concentrates (EC) and Gels (GF) are generally used for the treatment of plant propagation materials, in particular seeds. These compositions can be applied to plant propagation materials, in particular seeds, diluted or undiluted. The compositions in question produce, after a dilution of two to ten times, concentrations of active substance from 0.01 to 60% by weight, preferably from 0.1 to 40% by weight, in ready-to-use preparations. The application can be carried out before or during sowing. The methods for applying or treating agrochemical compounds and their compositions, respectively, in the plant propagation material, especially seeds, are known in the art and include application methods by coating, coating, pelting, dusting, soaking and grooving the material. of propagation. In a preferred embodiment, the compounds or their compositions, respectively, are applied to the plant propagation material by a method that does not induce germination, for example by coating, peeling, coating and sprinkling the seed.
In a preferred embodiment, a suspension type composition (FS) is used for the treatment of seeds. Typically, a FS composition may comprise 1-800 g / l of active substance, 1-200 g / l of surfactant, 0 to 200 g / l of antifreeze agent, 0 to 400 g / l of binder, 0 to 200 g / l of a pigment and up to 1 liter of a solvent, preferably water.
The carboxamide compounds according to the present invention can be used as such or in the form of their compositions, for example in the form of directly sprayable solutions, powders, suspensions, dispersions, emulsions, oil dispersions, pastes, dusts, materials to disperse, or granules, by means of spraying, atomizing, dusting, dispersing, brushing, dipping or shedding. The forms of application depend totally on the intended purposes; in each case it is intended to ensure that the distribution of the active substances according to the invention is as fine as possible.
Aqueous application forms can be prepared from emulsion concentrates, pastes or wettable powders (sprayable powders, oily dispersions) by the addition of water. To prepare emulsions, pastes or oily dispersions, the substances, either as such or dissolved in an oil or solvent, can be homogenized in water by means of a humectant, tackifier, dispersant or emulsifier. Alternatively, it is possible to prepare concentrates composed of active substance, humectant, tackifier, dispersant or emulsifier and, if appropriate, solvent or oil, and said concentrates are suitable for dilution with water.
The concentrations of active substance in ready-to-use preparations can vary within relatively wide ranges. In general, they range from 0.0001 to 10%, preferably from 0.001 to 1% by weight of active substance.
The active substances can also be used successfully in the ultra low volume process (ULV), it being possible to apply compositions comprising more than 95% by weight of the active substance, or even to apply the active substance without additives.
The amounts of active substances applied vary, according to the type of effect desired, from 0.001 to 2 kg per ha, preferably from 0.005 to 2 kg per ha, more preferably from 0.05 to 0.9 kg per ha, in particular from 0. , 1 to 0.75 kg per ha.
For the treatment of plant propagation materials, such as seeds, for example by dipping, coating or soaking seeds, amounts of active substance of 0.1 to 1000 g, preferably 1 to 1000 g, more preferably 1 are required. at 100 g and most preferably from 5 to 100 g, per 100 kilograms of plant propagation material (preferably seeds).
Various types of oils, humectants, adjuvants, herbicides, bactericides, other fungicides and / or pesticides can be added to the active substances or to the compositions comprising them, if appropriate not until immediately before use (mixing in the tank). These agents can be mixed with the compositions according to the invention in a weight ratio of 1: 100 to 100: 1, preferably 1: 10 to 10: 1.
The adjuvants that can be used are, in particular, modified organic polysiloxanes such as Break Thru S 240®; alcohol alkoxylates such as Atplus 245®, Atplus MBA 1303®, Plurafac LF 300® and Lutensol ON 30®; EO / PO block polymers, for example Pluronic RPE 2035® and Genapol B®; alcohol ethoxylates such as Lutensol XP 80®; and sodium dioctyl sulfosuccinate such as Leophen RA®.
The compositions according to the invention can also be present, in the form of use as fungicides, together with other active substances, for example with herbicides, insecticides, growth regulators, fungicides or else with fertilizers, as a premix, if appropriate, not until immediately before use (mixing in the tank).
In a preferred embodiment of the invention, the mixtures of the invention are used for the protection of the plant propagation material, for example the seeds and the roots and the shoots of the seedlings, preferably the seeds.
Seed treatment can be done in the seed box before planting in the field.
For seed treatment, the weight ratio in the binary, tertiary and quaternary mixtures of the present invention generally depends on the properties of the carboxamide compounds according to the present invention.
Compositions that are especially useful for the treatment of seeds are, for example: A soluble concentrates (SL, LS) D Emulsions (EW, EO, ES) E Suspensions (SC, OD, FS) F Water-dispersible granules and water-soluble granules (WG, SG) G Water-dispersible powders and water-soluble powders (WP, SP, WS) H Gel formulations (GF) I Powders that can be converted into powders (DP, DS) These compositions can be applied to plant propagation materials, in particular seeds, diluted or undiluted. These compositions can be applied to plant propagation materials, in particular seeds, diluted or undiluted. The compositions in question produce, after a dilution of two to ten times, concentrations of active substance from 0.01 to 60% by weight, preferably from 0.1 to 40% by weight, in ready-to-use preparations. The application can be carried out before or during sowing. The methods for applying or treating agrochemical compounds and their compositions, respectively, in the plant propagation material, especially seeds, are known in the art and include methods of application by coating, coating, pelting, dusting and soaking the propagation material ( and also treatment in furrow). In a preferred embodiment, the compounds or their compositions, respectively, are applied in the plant propagation material by a method that does not induce germination, for example by coating, peeling, coating and sprinkling the seed.
In the treatment of the plant propagation material (preferably seed), the application rates of the mixture of the invention are generally for the formulated product (which usually comprises from 10 to 750 g / l of the active (s).
The invention also relates to the plant propagation products, and especially the seed, which comprise, ie are coated and / or contain, a mixture as defined above or a composition containing the mixture of two or more active ingredients or a mixture of two or more compositions, wherein each provides one of the active ingredients. The plant propagation material (preferably seeds) comprises the mixtures of the invention in an amount of 0.1 g to 10 kg per 100 kg of plant propagation material (preferably seeds).
In one embodiment, the process of the present invention uses transgenic plants, their parts, cells or organelles.
For the purposes of the invention, "transgenic", "transgene" or "recipient" means, in relation for example to a nucleic acid sequence, an expression cassette, a gene construct or a vector comprising the nucleic acid sequence or a organism transformed with the sequences of nucleic acids, expression cassettes or vectors, all those constructions obtained by recombinant methods in which (a) nucleic acid sequences encoding proteins useful in the methods of the invention, or (b) sequence (s) of genetic control that is operably linked to the nucleic acid sequence according to the invention, for example a promoter, or (c) a) and b) they are not found in their natural genetic environment or were modified by recombinant methods, where it is possible that the modification is, for example, a substitution, addition, elimination, inversion or insertion of one or more nucleotide residues. Natural genetic environment means the natural chromosomal or genomic locus in the original plant and can be deduced from the presence in a genomic library. In the case of a genomic library, the natural genetic environment of the nucleic acid sequence is preferably retained, at least in part. The environment flanks the nucleic acid sequence on at least one side and has a sequence length of at least 50 bp, preferably at least 500 bp, preferably especially at least 1000 bp, most preferably at least 5000 bp. A natural expression cassette - for example the natural combination of the natural promoter of the nucleic acid sequences with the corresponding nucleic acid sequence - is transformed into a cassette of transgenic expression when this expression cassette is modified by non-natural, synthetic methods ( "artificial") such as, for example, mutagenic treatment. Suitable methods are described, for example, in US 5565350 or WO 2000/15815.
Therefore, for the purposes of the invention, a transgenic plant means, as indicated above, that the nucleic acids are not found in their natural locus in the genome of said plant, it being possible for the nucleic acids to be expressed in a homologous manner or heterologous However, transgenic also means that the nucleic acids according to the invention are located in their natural position in the genome of an organism, but that the sequence, for example, the coding sequence or a regulatory sequence, for example the sequence of the promoter, was modified compared to the natural sequence. Preferably, transgenic means the expression of the nucleic acids at a non-natural locus in the genome, i.e. homologous or, preferably, heterologous expression of the nucleic acids takes place. Preferred transgenic plants are mentioned herein.
These transgenic plants can be any listed in Table A, such as any of A- to A-56. Likewise, the transgenic plants used in the process of the invention can comprise as transgen one or several of the genes listed in Table B .
However, the process of the present invention is not limited to transgenic plants and also to these transgenic plants. Other transgenic plants suitable for the process of the present invention can be generated by methods known in the art. In the next sectionExemplary methods for producing transgenic plants suitable for the process of the present invention are exemplified in a non-limiting manner. The person skilled in the art knows that the methods used to produce the transgenic plants are not crucial for the use of said plants for carrying out the present invention.
The term "introduction" or "transformation", as indicated herein, encompasses the transfer of an exogenous polynucleotide to a host cell, regardless of the method used for the transfer. In particular, with respect to the transgenic plants, "transformation" or "transformed" preferably refers to the transfer of an exogenous polynucleotide to a host cell, independently of the method used for the transfer.
Transformation methods include the use of liposomes, electroporation, chemicals that increase the absorption of free DNA, injection of DNA directly into the plant, particle bombardment, transformation using viruses or pollen, and microprojection. The methods can be selected from the calcium / polyethylene glycol method for protoplasts (Krens, F.A. et al., (1982) Nature 296, 72-74; Negrutiu I et al. (1987) Plant Mol Biol 8: 363-373); protoplast electroporation (Shillito R.D. et al. (1985) Bio / Technol 3, 1099-1 102); microinjection in the plant material (Crossway A et al., (1986) Mol Gen Genet 202: 179-185); bombardment of particles coated with DNA or RNA (Klein TM et al., (1987) Nature 327: 70) virus infection (non-iritegrative) and the like. Transgenic plants, including transgenic crop plants, are preferably produced by Agrobacterium-mediated transformation.
For example, a suitable vector, for example a binary vector, can be transformed into a suitable Agrobacterium strain, for example LBA4044, according to methods known in the art. Said transformed Agrobacterium can then be used to transform plant cells, as described in the following examples.
Example I: Examples of transformation of plants Rice transformation The Agrobacterium that contains the expression vector is used to transform Oryza sativa plants. The husks of mature dry seeds are removed from the Japanese rice cultivar Nipponbare. Sterilization is performed by incubation for one minute in 70% ethanol, followed by 30 minutes in 0.2% HgCI2, followed by 6 washes of 15 minutes with sterile distilled water. The sterile seeds are then germinated in a medium containing 2,4-D (callus induction medium). After incubation in the dark for four weeks, embryogenic callus, scutelle derivatives, are extracted and propagated in the same medium. After two weeks, the calluses multiply or spread by subculture in the same medium for another 2 weeks. The embryogenic callus pieces are subcultured in fresh medium 3 days before of cocultivation (to stimulate cell division activity).
Agrobacterium strain LBA4404 containing the expression vector is used for cocultivation. Agrobacterium is inoculated in an AB medium with the appropriate antibiotics and cultivated for 3 days at 28 ° C. The bacteria are then collected and suspended in a liquid coculture medium at a density (OD600) of about 1. The suspension is then transferred to a Petri dish and the calli are immersed in the suspension for 15 minutes. The callus tissues are then dried on a filter paper and transferred to a solidified coculture medium, and incubated for 3 days in the dark at 25 ° C. The co-cultured calli are cultured in a medium containing 2,4-D for 4 weeks in the dark at 28 ° C in the presence of a selection agent. During this period, islands of resistant calluses develop rapidly. After transferring this material to a medium of regeneration and incubation to light, the embryogenic potential is released and shoots develop in the following four to five weeks. The callus shoots are removed and incubated for 2 to 3 weeks in an auxin-containing medium from which they are transferred to the soil. Hardened shoots are grown under high humidity conditions and short days in a greenhouse.
About 35 independent T0 rice transformants are generated for one construction. The primary transformants are transferred from a tissue culture chamber to a greenhouse. After a quantitative PCR analysis to verify the copy quantity of the T-DNA insert, only the single copy transgenic plants that are tolerant to the selection agent for harvesting the T1 seed are conserved. The seeds are then harvested three to five months after the transplant. The method produced single-locus transformants in a proportion of more than 50% (Aldemita and Hodges1996, Chan et al., 1993, Hiei et al., 1994).
About 35 independent TO rice transformations are generated. The primary transformants are transferred from a tissue culture chamber to a greenhouse for the cultivation and harvest of the T1 seed. Six events are retained, of which the progeny of T1 segregated 3: 1 for the presence / absence of the transgene. For each of these events, approximately 10 T1 seedlings containing the transgene (hetero- and homozygous) and approximately 10 T1 seedlings that do not have the transgene (nulicigotes) are selected to monitor the expression of the visual marker.
Corn transformation The transformation of corn (Zea mays) is carried out with a modification of the method described by Ishida et al. (1996) Nature Biotech 14 (6): 745-50. The transformation depends on the genotype in the maize and only specific genotypes can be transformed and regenerated. The inbred line A188 (University of Minnesota) or hybrids with A188 as a parent are good sources of donor material for transformation, but other genotypes can also be used successfully. The ears are harvested from the corn plant about 1 1 days after pollination (DAP) when the immature embryo has a length of about 1 to 1, 2 mm. The immature embryos are co-cultured with Agrobacterium tumefaciens which contains the expression vector, and the transgenic plants are recovered by means of organogenesis. The extracted embryos are grown in callus induction medium, then in corn regeneration medium, which contains the selection agent (for example, imidazolinone, but several selection markers can be used). Petri dishes are incubated in light at 25 ° C for 2-3 weeks or until buds develop. The green shoots are transferred from each embryo to the rooting medium of corn and incubated at 25 ° C for 2-3 weeks, until the roots develop. The shoots with roots are transplanted to soil in the greenhouse. T1 seeds are produced from plants that exhibit tolerance to the selection agent and that contain a single copy of the T-DNA insert.
Wheat transformation The transformation of the wheat is done with the method described by Ishida et al. (1996) Nature Biotech 14 (6): 745-50. The Bobwhite cultivar (available from CIMMYT, Mexico) is commonly used in processing. The immature embryos are co-cultured with Agrobacterium tumefaciens which contains the expression vector and the transgenic plants are recovered by means of organogenesis. After incubation with Agrobacterium, the embryos are cultured in vitro in callus induction medium, then in regeneration medium, which contains the selection agent (for example, imidazolinone, but several selection markers can be used). Petri dishes are incubated in light at 25 ° C for 2-3 weeks or until buds develop. The green shoots are transferred from each embryo to the rooting medium and incubated at 25 ° C for 2-3 weeks, until the roots develop. The shoots with roots are transplanted to soil in the greenhouse. T1 seeds are produced from plants that exhibit tolerance to the selection agent and that contain a single copy of the T-DNA insert.
Soybean transformation The soybean is transformed according to a modification of the method described in US Pat. No. 5,164,310 of Texas A &; M. Various varieties of commercial soybeans are susceptible to transformation with this method. The cultivar Jack (available from the Illinois Seed Foundation) is commonly used for processing. Soybeans are sterilized for in vitro planting. The hypocotyl, the radicle and a cotyledon of seven-day-old seedlings are extracted. The epicotyl and the remaining cotyledon are further cultured to develop axillary nodules. These axillary nodules are extracted and incubated with Agrobacterium tumefaciens which contains the expression vector. After the cocultivation treatment, the explants are washed and transferred to the selection medium. The regenerated shoots are removed and placed in a shoot extension medium. The shoots whose length does not exceed 1 cm are placed in the middle of rooting until the roots develop. The shoots with roots are transplanted to soil in the greenhouse. T1 seeds are produced from plants that exhibit tolerance to the selection agent and that contain a single copy of the T-DNA insert.
Rapeseed / canola transformation Cotyledonary petioles and hypocotyls of young 5-6 day old seedlings are used as explants for tissue culture and transformed according to Babic et al. (1998, Plant Cell Rep 17: 183-188). The commercial cultivar Westar (Agriculture Canada) is the standard variety used for processing, but other varieties can also be used. Canola seeds are sterilized on the surface for in vitro sowing. The explants of cotyledonary petioles with the cotyledon attached are extracted from the in vitro plantlets and inoculated with Agrobacterium (which contains the expression vector) by immersing the cut end of the petiole explant in the bacterial suspension. The explants are then cultured for 2 days in MSBAP-3 medium containing 3 mg / l of BAP, 3% of sucrose, 0.7% of Phytagar at 23 ° C, 16 hs of light. After two days of cocultivation with Agrobacterium, the petiole explants are transferred to MSBAP-3 medium containing 3 mg / l of BAP, cefotaxime, carbenicillin or timentin (300 mg / l) for 7 days, and then cultivated in medium. MSBAP-3 with cefotaxime, carbenicillin or timentina and agent of selection until the regeneration of the shoots. When the shoots are 5-10 mm in length, they are cut and transferred to shoot extension medium (MSBAP-0.5, which contains 0.5 mg / l BAP). The shoots of around 2 cm in length are transferred to the rooting medium (MSO) for the induction of roots. The shoots with roots are transplanted to soil in the greenhouse. T1 seeds are produced from plants that exhibit tolerance to the selection agent and that contain a single copy of the T-DNA insert.
Transformation of alfalfa An alfalfa regenerative clone (Medicago sativa) is transformed with the method of (McKersie et al., 1999 Plant Physiol 119: 839-847). The regeneration and transformation of alfalfa depend on the genotype and, therefore, a regenerative plant is required. Methods for obtaining regenerative plants have been described. For example, these can be selected from the cultivar Rangelander (Agriculture Canada) or from any other variety of commercial alfalfa as described by Brown DCW and A Atanassov (1985. Plant Cell Tissue Organ Culture 4: 1 1 1-1 12). Alternatively, variety RA3 (University of Wisconsin) was selected for use in tissue culture (Walker et al., 1978 Am J Bot 65: 654-659). The petiole explants are co-cultured, overnight, with a culture of Agrobacterium tumefaciens C58C1 pMP90 (McKersie et al., 1999 Plant Physiol 1 19: 839-847) or LBA4404 containing the expression vector. The explants are co-cultured for 3 days in the dark in SH induction medium containing 288 mg / L of Pro, 53 mg / L of thioproline, 4.35 g / L of K2S04 and 100 m of acetosyringinone. The explants are washed in medium concentration Murashige-Skoog medium (Murashige and Skoog, 1962) and plated on the same SH induction medium without acetosyrininone but with a suitable selection agent and suitable antibiotic to inhibit the growth of Agrobacterium. After several weeks, the somatic embryos are transferred to BOÍ2Y development medium that does not contain growth regulators, nor antibiotics and 50 g / L of sucrose. Subsequently, the somatic embryos are germinated in Murashige-Skoog medium concentration medium. The seedlings with roots are transplanted into pots and grown in a greenhouse. T1 seeds are produced from plants that exhibit tolerance to the selection agent and that contain a single copy of the T-DNA insert.
Cotton transformation The cotton is transformed using Agrobacterium tumefaciens according to the method described in US 5,159,135. The cotton seeds are sterilized on the surface in 3% sodium hypochlorite solution for 20 minutes and washed in distilled water with 500 mg / ml cefotaxime. The seeds are then transferred to the SH medium with 50 g / ml of benomyl for germination. The hypocotyls are extracted from the seedlings that have 4 to 6 days, cut into pieces of 0.5 cm and placed on 0.8% agar. A suspension of Agrobacterium (approximately 108 cells per ml, diluted from an overnight culture transformed with the gene of interest and suitable selection markers) is used for the inoculation of the hypocotyl explants. After 3 days at room temperature and light, the tissues are transferred to a solid medium (1.6 g / l Gelrite) with Murashige and Skoog salts with vitamins B5 (Gamborg et al., Exp. Cell Res. 50: 151 -158 (1968)), 0.1 mg / l of 2,4-D, 0.1 mg / l of 6-furfurylaminopurine and 750 pg / ml of MgCL2, and with 50 to 100 g / ml of cefotaxime and 400 -500 g / ml carbenicillin to eliminate residual bacteria. Individual cell lines are isolated after two to three months (with subcultures every four to six weeks) and further cultured in a selective medium for tissue amplification (30 ° C, 16 hr photoperiod). Subsequently, the transformed tissues are further cultured in non-selective medium for 2 to 3 months so that somatic embryos are generated. Healthy-looking embryos of at least 4 mm in length are transferred to tubes with SH medium in fine vermiculite, supplemented with 0.1 mg / l indole acetic acid, 6 furfurylaminopurine and gibberellic acid. The embryos are grown at 30 ° C with a photoperiod of 16 hours, and the seedlings in the 2 to 3 leaf stage are transferred to pots with vermiculite and nutrients. The plants become more resistant and later they are transferred to the greenhouse to continue the cultivation. # Transformation of Arabidopsis plants Approximately 30-60 ng of prepared vector and a defined amount of amplified preparation are mixed and hybridized at 65 ° C for 15 minutes followed by 37 ° C 0.1 ° C / 1 second, followed by 37 ° C 10 minutes, followed of 0, 1 ° C / 1 second, then 4-10 ° C.
The ligated constructs are transformed into the same reaction vessel by the addition of competent E. coli cells (strain DH5alpha) and incubation for 20 minutes at 1 ° C followed by thermal shock for 90 seconds at 42 ° C and cooling to 1 -4 ° C. Then, the complete medium (SOC) is added and the mixture is incubate for 45 minutes at 37 ° C. Subsequently, the whole mixture is placed on an agar plate with 0.05 mg / ml kanamycin and incubated overnight at 37 ° C.
The result of the cloning step is verified by amplification with the help of primers that are connected upstream and downstream of the integration site, thus allowing the amplification of the insertion. The amplifications are performed as described in the Taq DNA polymerase protocol (Gibco-BRL).
The amplification cycles are the following: 1 cycle of 1-5 minutes at 94 ° C, followed by 35 cycles, in each case, 15-60 seconds at 94 ° C, 15-60 seconds at 50-66 ° C and 5-15 minutes at 72 ° C , followed by 1 cycle of 10 minutes at 72 ° C, then 4-16 ° C.
Several colonies are controlled, but only a colony in which a PCR product of the expected size is detected is used in the following steps.
A portion of this positive colony is transferred to a reaction vessel filled with the complete medium (LB) supplemented with kanamycin and incubated overnight at 37 ° C.
The preparation of the plasmid is carried out as specified in the standard protocol Qiaprep or NucleoSpin Multi-96 Plus (Qiagen or acherey-Nagel).
Generation of transgenic plants 1-5 ng of the isolated plasmid DNA is transformed by electroporation or transformation into competent cells of Agrobacterium tumefaciens of the strain GV 3 01 pMP90 (Koncz and Schell, Mol.Gen.G204.383 (1986)). Then, the complete medium (YEP) is added and the mixture is transferred to a new reaction vessel for 3 hours at 28 ° C. Subsequently, the entire reaction mixture is placed on YEP agar plates supplemented with the respective antibiotics, for example rifampicin (0.1 mg / ml), gentamicin (0.025 mg / ml and kanamycin (0.05 mg / ml) and it is incubated for 48 hours at 28 ° C.
The agrobacteria that contain the plasmid construct are then used for the transformation of the plants.
A colony of the agar plate is collected with the aid of the tip of a pipette and absorbed in 3 ml of liquid TB medium, which also contains suitable antibiotics as described above. The preculture is cultured for 48 hours at 28 ° C 120 rpm. 400 ml of the LB medium containing the same antibiotics as indicated above for the main culture are used. The preculture is transferred to the main crop. It is grown for 18 hours at 28 ° C and 120 rpm. After centrifugation at 4,000 rpm, the pellet is resuspended in the infiltration medium (MS medium, 10% sucrose).
To grow the plants for transformation, plates are filled halfway (Piki Saat 80, green, provided with an observation filter, 30 x 20 x 4.5 cm, from Wiesauplast, Kunststofftechnik, Germany) with a GS 90 substrate ( standard floor, Werkverband EV, Germany). The plates are watered overnight with 0.05% Proplant solution (Chimac-Apriphar, Belgium). Seeds of Arabidopsis thaliana C24 (Nottingham Arabidopsis Stock Center, UK, NASC Stock N906) are dispersed on the plate, approximately 1000 seeds per plate. The plates are covered with a hood and placed in the stratification system (8 hours, 1 10 pmol / m2s1, 22 ° C, 16 hours, darkness, 6 ° C). After 5 days, the plates are placed in a controlled environment chamber for short days (8 hours, 130 pmol / m2s1, 22 ° C, 16 hours, darkness, 20 ° C), where they remain for approximately 10 days until they are they form the first true leaves.
The seedlings are transferred to pots containing the same substrate (Teku pots, 7 cm, LC series, manufactured by Póppelmann GmbH &Co., Germany). Five plants are transplanted to each pot. Then the pots return to the controlled environment chamber of short days so that the plant continues to grow.
After 10 days, the plants are transferred to the greenhouse cabinet (complementary lighting, 16 hours, 340 pE / m2s, 22 ° C, 8 hours, darkness, 20 ° C), where they are allowed to grow for 17 more days.
For the transformation, 6-week-old Arabidopsis plants, which had just started to flower, were immersed for 10 seconds in the suspension of agrobacteria described above that had been previously treated with 10 μ? of Silwett L77 (Crompton S.A., Osi Specialties, Switzerland). The method in question is described by Clough J.C. and Bent A.F. (Plant J. 16, 735 (1998)).
Then, the plants are placed in a humid chamber for 18 hours.
Later, the pots return to the greenhouse so that the plants continue to grow. The plants remain in the greenhouse for another 10 weeks until the seeds are ready for harvest.
According to the resistance marker used for the selection of the transformed plants, the harvested seeds are planted in the greenhouse and subjected to a selection by spray or first sterilized and then grown on agar plates supplemented with the respective selection agent. Because the vector contains the bar gene as resistance marker, the plants are sprayed four times in a range of 2 to 3 days with 0.02% BASTA® and the transformed plants are allowed to produce seeds.
The seeds of A. thaliana transgenic plants are stored in the freezer (at -20 ° C).
Example II: Application of a carboxamide compound selected from the group consisting of boscalid, (N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole- 4-carboxamide), bixafen, penflufen (N- [2- (1, 3-dimethylbutyl) -phenyl] -1, 3-dimethyl-5-fluoro-1 H -pyrazole-4-carboxamide), fluopyram, sedaxane, isopyrazam , pentiopyrad, benodanil, carboxin, fenfuram, flutolanil, furametpir, mepronil, oxycarboxine and tifluzamide.
II. A Seed treatments The control and cultivated corn seeds of the T2 generation are treated with deionized water (Blank), from 10 grams to 200 grams of a carboxamide compound; all formulation rates are grams / 100 kg of seed. Each formulation is applied to approximately 80 seeds. The formulation is pipetted into a 125 ml container along the sides and bottom of the container before adding the seeds and shaking the container for 30 seconds. The coated seeds are then removed from the container and placed on a plastic plate to dry.
Seventy-five 3-L pots are filled by treatment with potting media, labeled with colored sticks and assigned a unique bar code. One seed is planted per pot in a depth of about 2 cm and covered with the medium. The medium is gently watered to imbibe the seeds, while allowing ample oxygen exchange so that the chemical coatings of the seeds remain intact. After planting, the pots are randomly distributed in three replica blocks (1 bank = 1 block), each with 25 plants of each treatment.
The plants are kept in a greenhouse under optimal conditions and abundant irrigation (80-90% of the field capacity) after emergence. Supplemental nutrients are administered every three days during irrigation. The temperature of the greenhouse is maintained at 30 ° C, relative humidity 75% and light at 350 μ? a ?? m "2s" 1, in a photoperiod of 15 hours of day / 9 hours of night. Supplemental light is provided with metal halide lamps. Once a week, the pots are mixed randomly inside each block.
On day 21, the plants are photographed to collect the phenotypic data as described in WO2008 / 129060.
II. B Plant treatments The cultivation of plants, their treatment with fungicides and the evaluation of the fungicidal activity are known to those skilled in the art. The treatment of plants with carboxamides and the determination of infection after treatment are described, for example, in EP0545099, WO200307075, WO2006087343, WO200435589, EP846416, DE19629828, WO2003010149, EP1313709, JP 2000-342183, EP1 1 10956, WO200142223, WO2000. / 09482, WO200366609, WO200374491, WO200435555, WO200439799 and EP915868.
III. Evaluation III. A Procedure for evaluating rice plants subjected to the process of the present invention 1 Preparation of the evaluation The cultivated plants and the corresponding controls are grown side by side in random positions. The greenhouse conditions are of short days (12 hours of light), 28 ° C in the light and 22 ° C in the dark and relative humidity of 70%. Plants grown under stress-free conditions are irrigated at regular intervals to ensure that water and nutrients are not limiting and to meet the needs of plants to complete their growth and development.
From the sowing stage to the maturity stage, the plants are passed several times through a digital image taking cabinet. At each point of time, digital images (2048x1536 pixels, 16 million colors) of each plant are taken from at least 6 different angles. 2 Statistical analysis: Test F ANOVA (variant analysis) of two factors is used as a statistical model for the general evaluation of the phenotypic characteristics of the plant. An F test is carried out on all the measured parameters of all the plants. The threshold of significance for a global and true gene effect is set at a 5% probability level for the F test. 3 Measured parameters Measurement of parameters related to biomass From the sowing stage to the maturity stage, the plants are passed several times through a digital image taking cabinet. At each point of time, digital images (2048x1536 pixels, 16 million colors) of each plant are taken from at least 6 different angles.
The aerial area of the plant (or biomass of the foliage) is determined by counting the total number of pixels in the digital images of the aerial parts of the plants differentiated from the bottom. This value is averaged for photos taken at the same time point from different angles and converted to a physical surface value expressed in square mm per calibration. The experiments show that the aerial area of the plant measured in this way correlates with the biomass of the aerial parts of the plant. The aerial area is the area measured at the point of time at which the plant has reached its maximum foliage biomass. Early vigor is the aerial area of the plant (seedling) three weeks after germination. The increase in root biomass is expressed as an increase in the total biomass of the root (measured as the maximum root biomass observed during the life cycle of a plant); or as an increase in root / shoot index (measured as the ratio of root mass to shoot mass during the period of active root and shoot growth).
Early vigor is determined by counting the total number of pixels of the aerial parts of the plants differentiated from the bottom. This value is averaged for photos taken at the same time point from different angles and converted to a physical surface value expressed in square mm per calibration.
Measurement of parameters related to the seed The mature primary panicles are harvested, counted, bagged, labeled with bar codes and then dried for three days in an oven at 37 ° C. Then the panicles are threshed, and all the seeds are collected and counted. The filled shells are separated from the empty ones with an air blowing device. The empty shells are discarded and the remaining fraction is counted again. The full shells are weighed on an analytical balance. The amount of filled seeds is determined by counting the amount of filled shells remaining after the separation step. The total yield of the seeds is measured by weighing all the full husks harvested from a plant. The total amount of seeds per plant is measured by counting the amount of husks harvested from a plant. The weight of a thousand grains (TKW) is extrapolated from the amount of filled seeds counted and their total weight. The harvest index (Hl) in the present invention is defined as the ratio between the total yield of the seed and the aerial area (mm2), multiplied by a factor of 106. The total amount of flowers per panicle, as defined in present invention, is the relationship between the total amount of seeds and the quantity of mature primary panicles. The seed filling rate, as defined in the present invention, is the ratio (expressed as%) of the amount of filled seeds to the total amount of seeds (or florets).
Example III: B: Evaluation procedure of Arabidopsis plants subjected to the process of the present invention Analysis of plants to determine the increase in yield under standardized growth conditions.
In this experiment, an analysis of the plants can be performed to determine the yield increase (in this case: increased biomass yield) under standardized growth conditions in the absence of substantial abiotic stress. In a standard experiment, the soil is prepared as a 3.5: 1 (v / v) mixture of nutrient-rich soil (GS90, Tantau, Wansdorf, Germany) and quartz sand. Alternatively, plants can be planted in nutrient-rich soil (GS90, Tantau, Germany). The pots can be filled with soil mix and placed in trays. Water can be added to the trays so that the soil mix absorbs an adequate amount of water for the planting procedure. The seeds of transgenic plants of A. thaliana and their controls, for example non-transgenic wild type, can be planted in pots (6 cm in diameter). The stratification can be established for a period of 3-4 days in the dark at 4 ° C-5 ° C. Seed germination and growth can be initiated with a growth condition of 20 ° C and approximately 60% of Relative humidity, photoperiod of 16 hs and illumination with fluorescent light at approximately 200 mol / m2s.
In case the transgenic seeds are not uniformly transgenic, a selection step can be carried out, for example selection BASTA. This can be done on day 10 or day 1 1 (9 or 10 days after sowing) by spraying from the top of the seedlings that are in the pots. In the standard experiment, a 0.07% (v / v) solution of BASTA concentrate (183 g / l glufosinate-ammonium) can be sprayed once in tap water or, alternatively, a solution can be sprayed three times at 0.02% (v / v) of BASTA. Wild-type control plants can be sprayed only with tap water (instead of spraying them with BASTA dissolved in tap water), but in the remainder of the process they can be treated identically.
Plants can be individualized 13-14 days after sowing by removing excess seedlings and leaving a seedling in the soil. Transgenic events and control plants can be distributed evenly in the chamber.
Irrigation can be done every two days after removing the blankets in a standard experiment or, alternatively, every day.
The treatment with formulations of active ingredients can be carried out as described in this application or by any known method.
To measure the biomass yield, the fresh weight of the plant can be determined at the time of harvest (24-29 days after planting) when cutting and weighing the shoots. At the time of harvest, the plants may be in the pre-bloom stage and prior to the inflorescence growth. The transgenic plants can be compared with the wild-type, non-transgenic control plants, which can be harvested on the same day. The significance values for the statistical significance of the biomass changes can be calculated by applying the Student's t test (parameters: bilateral, unequal variation).
Two different types of experimental procedures are performed: -Procedure 1). 3-4 independent transgenic lines (= events) are evaluated per transgenic construct (22-30 plants per construct) and the biomass yield can be evaluated as described above.
-Procedure 2.) Up to five lines can be evaluated per transgenic construct at successive experimental levels (up to 4). Only the constructs that show a positive performance are subjected to the next experimental level. Usually, at the first level, five plants can be evaluated per construct and at subsequent levels 30-60 plants can be evaluated. The yield of the biomass can be evaluated as described above. The data of this type of experiment (Procedure 2) are indicated for the constructs that show the highest biomass yield, at least in two successive experimental levels.
The production of biomass can be measured by weighing the rosettes of the plants. The biomass increase can be calculated as the ratio between the average weight of the transgenic plants and the average weight of the control plants of the same experiment. The average biomass increase of the transgenic ones can be indicated (value of significance <0.3 and increase of biomass> 5% (proportion> 0.05)).
The yield of the seeds can be measured by collecting all the seeds of a plant and measuring the weight of a thousand grains. Various methods are known in the art.
IV. Evaluation procedure for pest control The person skilled in the art knows suitable methods for the inoculation and evaluation of infections for different plant species and types of pathogens. The following are examples that do not limit the present invention.
IV.A. Fungicidal control of rice fungus caused by Pyrícularía oryzae (Protective action) The leaves of the rice seedlings grown in pots are sprayed until fully soaked with an aqueous suspension, which contains the concentration of the active ingredient described above. The plants are allowed to air dry. The next day, the plants are inoculated with an aqueous spore suspension of Pyrícularía oryzae containing 1x106 spores / ml. The test plants are transferred immediately to a humid chamber. After 6 days at 22-24 ° C and relative atmospheric humidity close to 100%, the extent of the fungal attack on the leaves is visually assessed as% of the diseased area of the leaf.
IV.B Evaluation of susceptibility to soybean rust The fungus of soybean rust is a wild isolate of Brazil.
The plants are inoculated with P.pachyrhizi.
In order to obtain an adequate spore material for the inoculation, the soybean leaves that had been infected with the soybean rust 15-20 days before are removed 2-3 days before the inoculation and transferred to agar plates ( 1% agar in H20). The leaves are placed with the top side on the agar, which allows the fungus to grow through the tissue and produce very young spores. For the inoculation solution, the spores are detached from the leaves and added to a Tween-H20 solution. The spore count is performed with a light microscope using a Thoma counting chamber. For the inoculation of the plants, the spore suspension is added to a spray container of compressed air and applied uniformly to the plants or leaves until the surface of the leaves is considerably moistened. For microscopy, a density of 10x105 spores / ml is used. The inoculated plants are placed for 24 hours in a greenhouse chamber with an average temperature of 22 ° C and > 90% atmospheric humidity. The inoculated leaves are incubated in the same conditions in a Petri dish closed in 0.5% plant agar. The following cultivation process is carried out in a chamber with an average temperature of 250 and > 70% atmospheric humidity.
For the evaluation of the pathogenic development, the inoculated leaves of the plants are stained with blue aniline.
Blue aniline staining is used to detect fluorescent substances. During defensive reactions in interactions between hosts and in non-host interactions, substances such as phenols, callose or lignin accumulate or are produced, and are incorporated into the cell wall either locally in the papillae or in the cell complete (hypersensitivity reaction, HR). Complexes associated with blue aniline are formed, which generates, in the case of callose, yellow fluorescence. The sheet material is transferred to Falcon plates or tubes containing solution II that removes the dye (ethanol / acetic acid 6/1) and incubated in a water bath at 90 ° C for 10-15 minutes. Immediately afterwards the solution II that removes the tincture is removed and the leaves are washed 2x with water. For staining, the leaves are incubated for 1.5-2 hours in a staining solution (0.05% blue aniline = methyl blue, 0.067 M dipotassium acid phosphate) and analyzed immediately after microscopy.
The different types of interaction are evaluated (counted) by microscopy. An Olympus BX61 UV microscope (incident light) and a Longpath UV filter (excitation: 375/15, lightning splitter: 405 LP) are used. After staining with blue aniline, the spores look blue under UV light. Papillae can be recognized under fungal imprisonment by green / yellow staining. The hypersensitivity reaction (HR) is characterized by a total cellular fluorescence.
IV.C Evaluation of susceptibility to Phytophthora infestans Resistance to Phytophthora infestans can be evaluated, for example, in potatoes. Three different isolates of P. infestans are obtained from Plant Research International B.V. (Wageningen, the Netherlands).
Disease trials; leaf shedding For the leaf shedding test, leaves of plants grown for 6 to 12 weeks in the greenhouse are placed in pieces of floristry foam saturated with water, approximately 35x4x4 cm, and placed on a tray (40 cm wide, 60 cm long and 6 cm high) with perforated bottom. Each leaf is inoculated with two droplets (25 μm each) of sporangiospore solution on the abaxial side. Then, the tray is placed in a plastic bag on top of a tray, in which is placed a filter paper saturated with water, and incubated in a room of air conditioning at 17 ° C and a photoperiod of 16hs / 8hs day / night with fluorescent light (Philips TLD50W / 84HF and OSRAM L58W / 21-840). After 6 to 9 days, the leaves are evaluated to determine the development of disease symptoms caused by P. infestans.
Evaluation: It is considered that plants with leaves that clearly showed sporulation lesions 6 to 9 days after inoculation have a susceptible phenotype, while plants with leaves that showed no visible symptoms or necrosis on the side of the inoculation in the absence of a clear Sporulation are considered resistant.
IV.D Evaluation of susceptibility to Peronospora parasitica and Erysiphe cichoracearum The control of pathogenic fungi can be measured in Arabidopsis plants, for example by inoculation with the biotrophic fungus Peronospora parasitica or Erysiphe cichoracearum. a) Peronospora parasitic The plants of 5 to 8 weeks are sprayed with a suspension of spores (conidial spores, approximately 106 spores / ml).
The inoculated plants are covered with a plastic bag and kept overnight in conditions of humidity and darkness at 16 in a refrigerator. A day later, the plastic bag is opened for the first time and then, for example 6 hours later, it is completely removed. Six days after the inoculation, the plants are again placed in a plastic bag overnight. This induced sporulation. The next day, the leaves are checked to determine the appearance of Conidioforas. During the following days, the intracellular growth of the fungus results in mild chlorosis to severe necrosis in the leaves. These symptoms are quantified and evaluated to determine their importance. b) Erysiphe cichoracearum This biotrophic fungus is grown in Arabidopsis plants. To achieve infection, a soft, small brush is used to collect the Conidiophores from the infected leaves and transfer them to the leaves of 4-week-old plants. Then, these plants are incubated for 7 days at 20 ° C. Subsequently, the new Conidiophores will be visible and during the following days the chlorosis and necrosis will be visible. These symptoms are quantified and evaluated to determine their importance.
V. Results: The cultivated plants treated according to the method of the invention show greater health of the plant.
SAW. Procedure for evaluating plants subjected to the process of the present invention Experiments were carried out using carboxamide compounds BOSCALID and N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, subsequently referred to as COMPOUND 2 .
SOY In 2008, soybeans were grown in the BASF experimental station in Campiñas, San Antonio de Posse, Sao Paulo, Brazil. Soybean was planted at a planting rate of 300,000 plants per ha. The space between the rows was 45 cm. The size of the plot was 10 m2.
COMPOUND 2 was applied twice in growth stages 55/61 (BBCH) and 65/71 (BBCH) as an experimental emulsion concentrate (EC) containing 62.5 g of active ingredient per liter with a product ratio of 0.48 l / ha and 0.8 l / ha. The formulation was applied in a total spray volume of 150 l / ha.
The infection with Asian soybean rust (Phakopsora pachyrhizi) was evaluated 20 days after the last treatment when calculating the infected area of the leaf in 10 randomly selected plants per plot (Tab 1). Efficacy was calculated as% decrease in infected area of the leaf in the treatments compared to the untreated control: E = (1 -a / b) · 100 a corresponds to the infected area of the leaf of the plants treated in% and b corresponds to the infected leaf area of the plants (control) not treated in% An efficacy of 0 means that the infected area of the leaf of the treated plants corresponds to that of the untreated control plants; an efficacy of 100 means that the treated plants showed a reduction in the infected area of the leaf of 100%, that is, it was not possible to detect infection of the Asian soybean rust.
In addition, the test was harvested and the grain yield and thousand grain weight (TGW) were measured (Tab 1).
Tab. Ex VI-1: Effectiveness of COMPOUND 2 against soybean rust and effect of yield * 95% Control Infection (infected area of the leaf) As shown in table 1, COMPOUND 2 has a good activity against Asian soybean rust. When treating a variety of glyphosate-tolerant transgenic soy with COMPOUND 2, this activity increases more than anticipated by the individual effects of COMPOUND 2 and the transgenic variety, respectively, in the control of soybean rust.
In addition, the treatment with COMPOUND 2 results in higher grain yield compared to the untreated control. The weight of the harvested grain of the treated soybean also increases compared to the untreated one. With respect to the effectiveness against soybean rust, when the variety of transgenic soy is treated, the yield and grain weight is much higher than anticipated by the combination of the individual effects of both the treatment with pyraclostrobin and the transgenic variety .
Therefore, synergistic effects for disease control and grain yield can be observed when combining treatment with COMPOUND 2 with a variety of transgenic soybeans.
CORN In 2008, corn was grown in the BASF experimental station in Campinas, San Antonio de Posse, San Pablo, Brazil. The variety DKB 390 was sown at a planting rate of 60,000 plants per ha. The space between the rows was 80 cm. The size of the plot was 30 m2.
COMPOUND 2 was applied once the spikes emerged (growth stage 51/55, BBCH) as an experimental emulsion concentrate (EC) containing 62.5 g of active ingredient per liter with a dosage rate of 0.8 l / ha. The formulations were applied in a total spray volume of 200 l / ha.
The infection with common rust (Puccinia sorghi) was evaluated 28 days after treatment with COMPOUND 2 (Tab.2) when calculating the infected area of the leaf in 10 plants per plot chosen at random. Efficacy was calculated as% decrease in infected area of the leaf in the treatments compared to the untreated control: E = (1-a / b) > 100 a corresponds to the infected area of the leaf of the plants treated in% and b corresponds to the infected leaf area of the plants (control) not treated in% An efficacy of 0 means that the infected area of the leaf of the treated plants corresponds to that of the untreated control plants; an efficiency of 100 means that the treated plants showed a reduction in the infected area of the leaf of 100%, that is to say, it was not possible to detect infection of common rust.
The retention by green leaves in treated and control plants was evaluated by estimating the green leaf area 28 days after treatment in 10 plants per plot, chosen at random During maturity, the plants were harvested and the grain yield and thousand grain weight (TGW) were measured (Tab.2).
Tab. Ex. VI-2: Effectiveness of COMPOUND 2 against common rust, effect on retention of green leaf tissue, grain yield and grain weight.
* Control infection 8.8% (infected area of the leaf).
As shown in table 1, COMPOUND 2 has good activity against common rust in corn. This activity is increased when transgenic corn varieties tolerant to glyphosate and / or resistant to insects are treated with COMPOUND 2 more than what can be foreseen for COMPOUND 2 and the transgenic varieties, respectively, in the control of common rust. Plants treated with COMPOUND 2 also show increased green leaf area compared to control plants. Similarly, the transgenic plants treated with COMPOUND 2 show an increase in green leaf tissue that is greater than can be expected from the combination of the effects that can be observed when using a transgenic variety or treating conventional corn with the COMPOUND 2.
In addition, the treatment with COMPOUND 2 results in higher grain yield compared to the untreated control. The weight of the grain Harvested from the treated corn also increases compared to the untreated control. The increase in yield and grain weight is much greater when the transgenic maize variety is treated than expected from the combination of the individual effects of both the COMPOUND 2 treatment and the transgenic variety. Therefore, synergistic effects for disease control and grain yield can be observed when combining treatment with COMPOUND 2 with a variety of transgenic maize.
RICE In 2008, imidazolinone-tolerant rice (Clearfield ™) was grown in Washington, 7033 Highway 103, LA, USA. The CL 161 variety was planted at a rate of 134 kg / ha. The space between the rows was 18 cm. The size of the plot was 27.5 m2.
COMPOUND 2 was applied once sprouted (growth stage 32/34, BBCH) as an emulsion experimental concentrate (EC) containing 62.5 g of active ingredient per liter with a dosage rate of 0.8 l / ha. The formulation was applied in a total spray volume of 187 l / ha.
The infection with Rhizoctonia solani was evaluated 77 days after the treatment with COMPOUND 2 (Tab.3) when calculating the infected area of the leaf and frequency of infection in 10 plants per plot chosen at random. Efficacy was calculated as% decrease in infected area of the leaf in the treatments compared to the untreated control: E = (1-a / b) «100 a corresponds to the infected area of the leaf of the plants treated in% and b corresponds to the infected leaf area of the plants (control) not treated in% An efficacy of 0 means that the infected area of the leaf of the treated plants corresponds to that of the untreated control plants; an efficacy of 100 means that the treated plants showed a reduction in the infected leaf area of 100%, ie no infection with Rhizoctonia solani could be detected.
During maturity, the plants were harvested and grain yield was measured (Tab 3).
Tab. Ex. VI-3: Efficacy of COMPOUND 2 against Rhizoctonia and effect of performance As shown in Table 3, COMPOUND 2 is active against Rhizoctonia in rice. This activity is higher in the imidazolinone tolerant rice variety when treated with COMPOUND 2 compared to a variety that does not have this herbicide tolerance trait.
In addition, treatment with COMPOUND 2 results in higher grain yield compared to the untreated control. The increase in grain yield is greater when the Clearfield ™ variety is treated than in a conventional variety.
The increase in efficacy in disease control and yield in the herbicide tolerant CL 161 variety is greater than that predicted by the effects of the treatment with COMPOUND 2 in a conventional rice variety and the herbicide tolerance trait in the CL 161 variety with respect to disease control and yield. Therefore, synergistic effects for disease control and grain yield can be observed when combining treatment with COMPOUND 2 with an imidazolinone tolerance trait.
COLZA OLEAGINOSA In 2002, oilseed rape was grown in Verrie in France. The Colosse variety was sown at a sowing rate of 3 kg / ha. The space between the rows was 17 cm. The size of the plot was 30 m2.
BOSCALID was applied once in the growth stage 16 (BBCH) using the commercially available Cantus (WG) formulation containing 500 g of active ingredient per kg with a dosage rate of 0.5 l / ha. The formulation was diluted in a total spray volume of 300 l / ha.
Infection with Leptosphaeria maculans 209 days after treatment with BOSCALID was evaluated (Tab.4) in the growth stage 75 (BBCH) of the culture. The stems of 50 plants were classified and the number of plants without symptoms (H1), less severe symptoms (H2), severe symptoms (H3) and very severe symptoms (H4) were counted. An index of diseases calculated as the average of the number of heavy plants through the four classes: (1 * Number of plants in H1 +2 * Number of plants in H2 + 3 * Number of plants in H3 + N0 of plants in H4) / Total number of plants evaluated The retention of green leaves in treated and control plants was evaluated by estimating the green leaf area 28 days after treatment in 10 plants per plot, chosen at random.
During maturity, the plants were harvested and grain yield was measured (Tab 4).
Tab. Ex. VI-4: Efficacy of BOSCALID against Leptosphaeria maculans (LEPTMA) and effect on yield Infection rate in control 49% As shown in table 4 BOSCALID has good activity against Leptosphaeria maculans in oilseed rape. The activity is greater is treated a variety of oilseed rape tolerant to herbicides with BOSCALID than expected from the combination of the effect of the BOSCALID treatment and the herbicide tolerant trait (resistance to imidazolinone), respectively, in the control of Leptosphaeria.
In addition, treatment with BOSCALID results in a higher grain yield compared to the untreated control. The increase in grain yield is greater when treating the herbicide-tolerant oilseed rape variety than expected for the combination of individual effects of both the BOSCALID treatment and the herbicide tolerance trait.
Therefore, synergistic effects for disease control and grain yield can be observed when treatment with BUSCALID is combined with a variety of oil-tolerant oilseed rape.

Claims (1)

  1. CLAIMS A method for controlling pests and / or increasing the health of the plants of a cultivated plant compared to the respective control, characterized in that it comprises the application of a pesticide to a plant with at least one modification, parts of said plant, propagation material plant or its growth locus, wherein the pesticide is selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1H- pyrazole-4-carboxamide, bixafen, penflufen, fluopyram, sedaxane, isopyrazam, pentiopyrad, benodanil, carboxy, fenfuram, flutolanil, furametpir, mepronil, oxycarboxine and thifluzamide. The method according to claim 1, characterized in that the pesticide is selected from the group consisting of boscalid, N- (3 ', 4', 5'-trifluorobiphenyl-2-yl) -3-difluoromethyl-1-methyl-1 H-pyrazole-4-carboxamide, bixafen, N- [2- (1, 3-dimethylbutyl) -phenyl] -1, 3-dimethyl-5-fluoro-1 H -pyrazole-4-carboxamide, fluopyram, sedaxane, isopyrazam and pentiopy. The method according to any of claims 1 or 2, characterized in that "higher plant health" means an increase, compared to the respective control, in a trait selected from the group consisting of yield, vigor of the plant, vigor early, effect of greater greenness, quality, tolerance to environmental stress, herbicide tolerance, insect resistance, fungal resistance or viral resistance or bacterial resistance, resistance to antibiotics, fine chemical content advantageous for application in the field of industry food for humans and / or animals, the cosmetic industry or the pharmaceutical industry, efficiency in the use of nutrients, use of absorbed nutrients, quality of the fiber, color and sterility of the male and / or "greater health of the plant" means an alteration or modification, in comparison with the respective control, in a feature selected from the group consisting of maturation, restoration, of fertility and color. The method according to claim 1 or 2, characterized in that the cultivated plant shows at least one of the following properties: tolerance to herbicides, insect resistance, fungal resistance or viral resistance or bacterial resistance, tolerance to stress, alteration of the maturation, modification of the content of the chemical products present in the plant cultivated, modification of nutrient uptake, antibiotic resistance and sterility of the male compared to the corresponding control plant, respectively. The method according to any of claims 1 to 4, characterized in that the plant is tolerant to the action of the herbicides. The method according to any of claims 1 to 5, characterized in that the plant is tolerant to the action of glyphosate. The method according to any of claims 1 to 5, characterized in that the plant is tolerant to the action of glufosinate. The method according to any of claims 1 to 5, characterized in that the plant is tolerant to the action of the imidazolinone herbicides. The method according to any of claims 1 to 5, characterized in that the plant is tolerant to the action of dicamba. The method according to any of claims 1 to 5, characterized in that the plant is capable of synthesizing at least one toxin that acts selectively derived from Bacillus spp. The method according to any of claims 1 to 5, characterized in that the plant is capable of synthesizing at least one toxin that acts selectively of Bacillus thuringiensis. The method according to any of claims 1 to 5, characterized in that the plant is capable of synthesizing one or more delta-endotoxin toxins that act selectively of Bacillus thuringiensis. The method according to any of claims 1 to 12, characterized in that the pesticide is applied to the plant propagation material of the cultivated plant. The method according to any of claims 1 to 12, characterized in that the treatments are carried out by applying at least one pesticide to the plant with at least one modification or its habitat. Seed of a cultivated plant as defined in any of claims 3 to 12 with at least one property as defined in claim 3 or 4 characterized in that it is treated with a pesticide as defined in claim 1 or 2. A composition characterized in that it comprises a pesticide as defined in claim 1 or 2 and a cultivated plant or its parts or cells. A method according to any of claims 1 to 14, the seed according to claim 15 or the composition according to the claim 16, characterized in that the cultivated plant is a transgenic plant. A method according to any of claims 1 to 14, a seed as defined in claim 15 or a composition as defined in claim 16, characterized in that the cultivated plant is a modified plant. A method for the production of an agricultural product characterized in that it comprises the application of a pesticide as defined in claim 1 or 2, to a plant cultivated with at least one modification, parts of said plant, plant propagation materials or its locus of growth, and the production of the agricultural product from said plant or parts of said plant or plant propagation material. The method according to claim 19, characterized in that the plant is defined as in any of claims 3, 5 to 12, 17 or 18. Use of a composition as defined in claim 16, characterized in that it is for the production of an agricultural product. Use of a pesticide as defined in claim 1 or 2, characterized in that it is for controlling pests and / or increasing the health of a cultivated plant as compared to the respective control. Use of a pesticide as defined in claim 1 or 2, characterized in that it is for controlling pests and / or increasing the health of the plants of a transgenic plant compared to the respective control.
MX2011004274A 2008-10-21 2009-10-21 Use of carboxamides on cultivated plants. MX343039B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08167079 2008-10-21
PCT/EP2009/063781 WO2010046380A2 (en) 2008-10-21 2009-10-21 Use of carboxamides on cultivated plants

Publications (2)

Publication Number Publication Date
MX2011004274A true MX2011004274A (en) 2011-05-24
MX343039B MX343039B (en) 2016-10-21

Family

ID=40380378

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011004274A MX343039B (en) 2008-10-21 2009-10-21 Use of carboxamides on cultivated plants.

Country Status (10)

Country Link
US (3) US20110203018A1 (en)
EP (1) EP2361015A2 (en)
JP (1) JP2012506373A (en)
KR (1) KR20110073610A (en)
BR (1) BRPI0914500A2 (en)
CA (1) CA2739153C (en)
EA (2) EA023545B1 (en)
MX (1) MX343039B (en)
UA (1) UA106052C2 (en)
WO (1) WO2010046380A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009211416A1 (en) * 2008-02-05 2009-08-13 Basf Se Plant health composition
CN101965128B (en) * 2008-02-05 2014-10-01 巴斯夫欧洲公司 Plant health composition
EP2105049A1 (en) * 2008-03-28 2009-09-30 Bayer CropScience AG Method of plant growth promotion using amide compounds
JP2013512934A (en) 2009-12-08 2013-04-18 ビーエーエスエフ ソシエタス・ヨーロピア Pesticide mixture
JP5706179B2 (en) * 2010-08-20 2015-04-22 住友化学株式会社 Harmful arthropod control composition and harmful arthropod control method
CA2827304C (en) * 2011-02-17 2018-11-13 Bayer Intellectual Property Gmbh Use of sdhi fungicides on conventionally bred asr-tolerant, stem canker resistant and/or frog-eye leaf spot resistant soybean varieties
US9510594B2 (en) 2011-02-17 2016-12-06 Bayer Intellectual Property Gmbh Use of SDHI fungicides on conventionally bred ASR-tolerant, stem canker resistant and/or frog-eye leaf spot resistant soybean varieties
WO2012125468A2 (en) * 2011-03-11 2012-09-20 Syngenta Participations Ag Enhanced seed treatments using oils
EP2524596A1 (en) * 2011-05-18 2012-11-21 Basf Se Seed treatment uses
JP6420027B2 (en) * 2013-05-28 2018-11-07 日産化学株式会社 Composition for controlling plant diseases and method for controlling plant diseases
CN103518725A (en) * 2013-10-14 2014-01-22 安徽丰乐农化有限责任公司 Bactericide taking boscalid as effective component and for rice field, wheat field and corn field
US10058542B1 (en) 2014-09-12 2018-08-28 Thioredoxin Systems Ab Composition comprising selenazol or thiazolone derivatives and silver and method of treatment therewith
BR112017021450B1 (en) 2015-04-07 2021-12-28 Basf Agrochemical Products B.V. PEST CONTROL METHODS, PLANT HEALTH IMPROVEMENT METHOD AND COATED SEED
CN105746527A (en) * 2016-04-22 2016-07-13 南京华洲药业有限公司 Fungicidal composition containing isopyrazam and boscalid and application thereof
EP3054014A3 (en) * 2016-05-10 2016-11-23 BASF Plant Science Company GmbH Use of a fungicide on transgenic plants
WO2019068810A1 (en) * 2017-10-06 2019-04-11 Bayer Aktiengesellschaft Use of compositions comprising fluopyram for enhancing antioxidative fitness of plants
CN109379920B (en) * 2018-09-21 2021-11-02 湖南省植物保护研究所 Method for rapidly breaking barnyard grass seed dormancy and improving germination rate

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8320535D0 (en) * 1983-07-29 1983-09-01 Elliott T J Mushroom mutant strain
IL103614A (en) * 1991-11-22 1998-09-24 Basf Ag Carboxamides for controlling botrytis and certain novel such compounds
DE4231517A1 (en) * 1992-09-21 1994-03-24 Basf Ag Carboxylic acid anilides, process for their preparation and compositions containing them for controlling harmful fungi
DE19735224A1 (en) * 1997-08-15 1999-02-18 Basf Ag New bi:phenyl-amide derivatives are active against wide range of phytopathogenic fungi
MY138097A (en) * 2000-03-22 2009-04-30 Du Pont Insecticidal anthranilamides
DE10136065A1 (en) * 2001-07-25 2003-02-13 Bayer Cropscience Ag pyrazolylcarboxanilides
TW200724033A (en) * 2001-09-21 2007-07-01 Du Pont Anthranilamide arthropodicide treatment
DE10215292A1 (en) * 2002-02-19 2003-08-28 Bayer Cropscience Ag New N-biphenylyl-1-methyl-3-(di- or trifluoromethyl)-1H-pyrazole-4-carboxamides, useful as microbicides, especially fungicides and bactericides for protection of plants or materials such as wood
DE10229595A1 (en) * 2002-07-02 2004-01-15 Bayer Cropscience Ag phenylbenzamides
OA19202A (en) * 2002-08-12 2006-10-13 Trinity Bay Equipment Holdings, LLC Protector assembly for flexible pipe coils and method of using same.
DE10303589A1 (en) * 2003-01-29 2004-08-12 Bayer Cropscience Ag pyrazolylcarboxanilides
DE10325438A1 (en) * 2003-05-21 2004-12-09 Bayer Cropscience Ag Difluormethylbenzanilide
EP2929779A3 (en) * 2003-12-15 2016-06-01 Monsanto Technology LLC Corn plant mon88017 and compositions and methods for detection thereof
DE502005009089D1 (en) * 2004-06-18 2010-04-08 Basf Se 1-METHYL-3-TRIFLUOROMETHYL-PYRAZOLE-4-CARBOXYLIC ACID (ORTHO-PHENYL) -ANILIDES AND THEIR USE AS FUNGICIDES
EP1761498A1 (en) * 2004-06-18 2007-03-14 Basf Aktiengesellschaft 1-methyl-3-difluoromethyl-pyrazol-4-carbonic acid-(ortho-phenyl)-anilides, and use thereof as a fungicide
DE102004029972A1 (en) * 2004-06-21 2006-01-05 Bayer Cropscience Ag Mordant for combating phytopathogenic fungi
US8455719B2 (en) * 2004-08-16 2013-06-04 Cropdesign N.V. Method for increasing seed yield or biomass by expressing RNA binding proteins in transgenic plants
CA2588243C (en) * 2004-09-29 2013-06-11 Pioneer Hi-Bred International, Inc. Corn event das-59122-7 and methods for detection thereof
GB0422401D0 (en) * 2004-10-08 2004-11-10 Syngenta Participations Ag Fungicidal compositions
DE102004049761A1 (en) * 2004-10-12 2006-04-13 Bayer Cropscience Ag Fungicidal drug combinations
AR051865A1 (en) * 2004-12-24 2007-02-14 Cropdesign Nv PLANTS HAVING GREATER PERFORMANCE AND METHOD FOR PRODUCERS
DE102005007160A1 (en) * 2005-02-16 2006-08-24 Basf Ag Pyrazolecarboxylic acid anilides, process for their preparation and compositions containing them for controlling harmful fungi
DE102005009458A1 (en) * 2005-03-02 2006-09-07 Bayer Cropscience Ag pyrazolylcarboxanilides
CA2602768A1 (en) * 2005-03-25 2006-09-28 Cropdesign N.V. Plants having increased yield and a method for making the same
DE102005015850A1 (en) * 2005-04-07 2006-10-12 Bayer Cropscience Ag Synergistic fungicidal drug combinations
PL1876889T3 (en) * 2005-04-25 2011-04-29 Syngenta Participations Ag Fungicidal aqueous compositions for seed treatment
DE102005022147A1 (en) * 2005-04-28 2006-11-02 Bayer Cropscience Ag Active agent combination, useful to e.g. combat animal parasites and phytopathogenic fungus, comprises a carboxyamide compound and/or at least an active agent e.g. acetylcholine-receptor-agonist/antagonist
DK1912503T3 (en) * 2005-08-05 2014-11-03 Basf Se FUNGICIDE MIXTURES CONTAINING SUBSTITUTED 1-METHYL-PYRAZOL-4-YLCARBOXYL ACIDANILIDES
CN101272688B (en) * 2005-08-09 2013-01-30 先正达参股股份有限公司 Method for controlling phytopathogenic organisms
AR057091A1 (en) * 2005-08-24 2007-11-14 Pioneer Hi Bred Int COMPOSITIONS THAT PROVIDE TOLERANCE TO MULTIPLE HERBICIDES AND METHODS TO USE THEM
US8853492B2 (en) * 2005-11-07 2014-10-07 Cropdesign N.V. Plants having improved growth characteristics and a method for making the same
MX2008012707A (en) * 2006-04-06 2008-10-10 Syngenta Participations Ag Fungicidal compositions.
DE102006024925A1 (en) * 2006-05-24 2007-11-29 Bayer Cropscience Ag Fungicidal drug combinations
US7884262B2 (en) * 2006-06-06 2011-02-08 Monsanto Technology Llc Modified DMO enzyme and methods of its use
DE102006030739A1 (en) * 2006-06-30 2008-01-03 Bayer Cropscience Ag Synergistic insecticidal and fungicidal mixtures
US8207092B2 (en) * 2006-10-16 2012-06-26 Monsanto Technology Llc Methods and compositions for improving plant health
EP1922927A1 (en) * 2006-10-26 2008-05-21 Syngeta Participations AG A method for controlling soybean rust
KR20090111336A (en) * 2007-01-19 2009-10-26 바스프 에스이 Fungicidal mixtures of 1-methylpyrazole-4-ylcarboxylic acid anilides and azolopyrimidinylamines
KR20100113157A (en) * 2008-02-05 2010-10-20 바스프 에스이 Pesticidal mixtures
CN101965128B (en) * 2008-02-05 2014-10-01 巴斯夫欧洲公司 Plant health composition
EP2039772A2 (en) * 2009-01-06 2009-03-25 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants introduction
AU2010212955A1 (en) * 2009-02-11 2011-09-01 Basf Se Pesticidal mixtures

Also Published As

Publication number Publication date
EA201300778A1 (en) 2013-11-29
KR20110073610A (en) 2011-06-29
CA2739153A1 (en) 2010-04-29
EA201100624A1 (en) 2011-12-30
CA2739153C (en) 2019-09-10
MX343039B (en) 2016-10-21
US20110203018A1 (en) 2011-08-18
JP2012506373A (en) 2012-03-15
US20140357485A1 (en) 2014-12-04
US20180116218A1 (en) 2018-05-03
EA023545B1 (en) 2016-06-30
EP2361015A2 (en) 2011-08-31
EA019439B1 (en) 2014-03-31
BRPI0914500A2 (en) 2015-08-11
WO2010046380A3 (en) 2011-04-14
UA106052C2 (en) 2014-07-25
WO2010046380A2 (en) 2010-04-29

Similar Documents

Publication Publication Date Title
CA2739153C (en) Use of carboxamides on cultivated plants
WO2010046422A2 (en) Use of auxin type herbicides on cultivated plants
WO2010046387A2 (en) Use of a respiration complex iii inhibitor on cultivated plants
CN101668860B (en) Plant produtivity enhancement by combining chemical agents with transgenic modifications
WO2010046417A1 (en) Use of growth inhibitor herbicide on cultivated plants
WO2010046463A1 (en) Use of selected insecticides on cultivated plants
EP3458591A1 (en) Dual transit peptides for targeting polypeptides
TW201031329A (en) Active compound combinations
KR100500751B1 (en) Method for protecting plants
WO2010046437A2 (en) Use of acetyl coa carboxylase inhibitors on cultivated plants
EP2822387A1 (en) Agrochemical compositions
WO2010046434A1 (en) Use of bleacher herbicides on cultivated plants
WO2010046419A2 (en) Use of acetolactate synthase inhibitors on cultivated plants
US20170121731A1 (en) New insecticidal protein
WO2010046389A2 (en) Use of oomycetes fungicide on cultivated plants
WO2010046430A2 (en) Use of photosystem ii inhibitors on cultivated plants
WO2010046420A2 (en) Use of protoporphyrinogen oxidase inhibitors on cultivated plants
WO2010046385A2 (en) Use of a sterol biosynthesis inhibitors on cultivated plants
WO2010046423A2 (en) Use of sulfonylurea herbicides on cultivated plants
WO2010046427A1 (en) Use of neonicotinoides on cultivated plants
WO2010046378A2 (en) Use of carboxylic amide fungicides on transgenic plants
WO2020246327A1 (en) Plant disease prevention method
WO2010046421A1 (en) Use of GABA-gated chloride channel antagonists on cultivated plants
WO2010015634A1 (en) Method for screening for a plant protectant
WO2010046382A2 (en) Use of sodium channel modulators on cultivated plants

Legal Events

Date Code Title Description
FG Grant or registration