KR20130080667A - Insecticidal composition containing piper spp. and lavandula spp - Google Patents

Abstract

PURPOSE: A composition containing a piper genus plant and a lavandula genus plant extract for killing insects is provided to be effectively used as an effective and environment-friendly pest controlling agent since the composition has an excellent pest controlling activity with respect to pests severely damaging a greenhouse cultivation land or an outdoor package. CONSTITUTION: A composition for pest control comprises at least one selected from the group consisting of an extract of Piper species, an extract of Lavandula species, and a mixed extract thereof as an active ingredient. The mixed extract of Piper species and Lavandular species is a mixture of Piper species and Lavandular species or a mixture of extracts of Piper species and Lavandular species. The extract of Piper species, the extract of Lavandula species, or the mixed extract thereof, is at least one selected from the group consisting of a plant oil extracted from Piper species, Lavandula species, or a mixture thereof by using a steam distillation method and a crude extract that is obtained by extracting with at least one solvent selected from the group consisting of water, a C1-C4 linear or branched alcohol, chloroform, and hexane. [Reference numerals] (AA) Control group; (BB) Emulsion composition of a Piper spp. extract; (CC) Emulsion composition of lavender essential oil; (DD) Emulsion composition of lavender and Piper spp. mixture; (EE) Water treatment; (FF,GG,HH) Sample (dilute 100 times/0.3% (v/v))

Description

Insecticidal composition containing pepper plant and Lavandula plant extracts Insecticidal composition containing Piper spp. and lavandula spp.}

The present invention is a pepper genus ( Piper spp .) Plant extract, Lavandula spp .) A pest control composition comprising a plant extract, and at least one extract selected from the group consisting of a mixture of a plant of the genus Pepper and Lavandula as an active ingredient, a pest control product comprising the same, and the pest control product It relates to a method for controlling pests comprising the step of applying the composition to a crop.

Plutella xylostella is a very small pest belonging to the family Lepidoptera, which adds mainly cruciferous crops such as cabbage, radish and cabbage, and is also called parachute by some farms. Chinese cabbage moth is a global pest that is widely distributed from tropical or subtropical regions of Southeast Asia such as the Philippines and Thailand to high latitudes of northern Canada, and the young insects (larvae) are cuticles on the back of the leaves of host crops such as cabbage, cabbage and radish. Dig into and leave only the epidermis to cool the flesh. In particular, when this pest occurs a lot during the seedling period of the crop, it eats young leaves and leaves only leaf veins, which inhibits the growth of cabbage and leads to significant deaths.

Chinese cabbage moth began to be a problem in the mid 1980s, and it started to occur in vegetable cultivation complex near some cities. The main reason for the increase in the occurrence of Chinese cabbage moths is that the growing area of cabbage, cabbage, radish, etc., which are the main vegetables of cruciferous plants, has recently increased, and the feeding conditions have been improved as the planting of cruciferous vegetables through the cultivation of facilities such as vinyl houses is carried out year-round. However, as the cultivation of cruciferous fruits and vegetables has become simple and continuous, natural enemies have been reduced due to the intensive spraying of drugs for pest control of vegetables such as aphids. It is developed and the drug control effect is reduced. Damage due to the increase in the occurrence of the Chinese cabbage moth is increasing.

Spodoptera exigua , also known as the beet thief moth, is a pest belonging to the Lepidoptera night moth family. Pabambang moth is a pest that causes sugar beet in Sariwon, Hwanghae Province in 1926. Since it was recorded for the first time in Korea, there have been few reports of occurrence and damage, but since 1986, the amount has increased rapidly, mainly in field crops in the southern regions. In particular, the most serious pests in green onion cultivation have been reported to be incapable of growing green onions. Although it is the largest pest of leeks, it is an omnivorous pest that affects almost all plants such as vegetables, flowers, crops, crops, and weeds. In particular, the pest has a strong resistance to drugs and can move over a long distance, and the amount of occurrence is increasing recently. It is anticipated that the occurrence of beetroot moth will be further expanded at the present time when there is no proper control against beetroot moth.

In general, the damage caused by pabam moths is when young larvae spawn 20 to 50 eggs on a leaf surface and hatch young larvae. It enters and leaves only the epidermis from the inside, and when it is 4-5 years old, it appears as a form of perforation through a large hole in the whole leaf.

Blue chestnut moth is known as a pest that is highly resistant to drugs and is recognized as a pest that is difficult to control in Korea. In young larvae, the age of 1-2 is relatively sensitive to drugs. After 3 years of age, homeless larvae become more resistant to drugs and, on the other hand, are added to the stem and are less likely to be exposed to drugs. In most cases, the drug against pabam moth is sprayed in the middle of July, early August and early September, 10 days after the adult stage, and chlorfenapyr, bifenthrinth, etofenprox emulsion, yotokon oil, diadia Nine kinds of pesticides, including pliers uron liquid dehydrating agent, chlorfenapyr emulsion, silafurouphene, mep emulsion, hexapromuron liquid dehydrating agent, flufenoxuron dispersing liquid preparation, bifenthrin, grofoaming agent, It is registered.

Tobacco Seminar ( Spodoptera) litura (Fabricius) did not cause any damage to crops except in some parts of Gyeongnam until the early 1980s, but since the late 1980s, the density of wintering adults has increased due to the increase in plant cultivation area. Natural pests have become a major pest in recent years for the elimination of natural enemies. Tobacco germinating moth is a pest that feeds on vegetables such as cabbages, beans, radishes, potatoes, strawberries, and other crops such as roses and carnations, and adds about 40 and more than 100 species of plants. The pests occur throughout the year in warming facilities, especially in the southern regions, and are estimated to pass five generations a year. Adult development peaks are estimated in early May, mid-June, late July, late August, and late September. Eggs are 7 days, larvae 1 to 6 years old 13, pupa 10 to 13 days, the adult lifespan is 10 to 15 days, spawning eggs 1,800 eggs in egg yolk, the eggs are covered with scales and the like. Young larvae damage the back of the leaf or shoots, and the grown larvae sneak into the ground during the day or lie under the fallen leaves, and then add leaves and buds at night. Should be.

In order to control tobacco castor moths in Korea, grofohydrate, bifenthrin and grofohydrate, etofenprox emulsion, etofenprox and hydrophobic, chlorfenapyr, bifenthrin, hexafururon, and grofohydrate are registered. have. When controlling drugs, the emergence of resistant individuals is difficult, which makes it difficult to control them. The most important thing in drug control is the spraying of young larvae when they occur. Other biological control agents include parasites (cocoa bees, nibble, cane bees), predators (13 species including wasps), microorganisms (nuclear polyhedron virus (NPV), bacteria (Bt), fungi), nematodes, etc.) .

Considering the extent of damage and drug resistance of the pests as described above, it can be said that the development of drugs that are not resistant to all these pests and exhibit effective pesticidal activity.

The inventors of the present invention have been concerned that the pests of plant extracts of the pepper and plant of the genus Lavandula against agricultural pests such as Chinese cabbage moth, pabam moth and tobacco germweed moth are severely damaged against various crops as agricultural pests and have strong resistance to drugs. Confirmed that the extract has excellent pesticidal activity to complete the present invention.

Thus, one example of the present invention is a pepper genus ( Piper spp .) Plant extract, Lavandula spp .) It provides a pest control composition containing a plant extract, and at least one extract selected from the group consisting of a mixture of the plant of the genus Pepper and Lavandula as an active ingredient and pest control products comprising the same.

Another example provides a pest control method comprising applying the pest control composition to a crop.

One example of the present invention

Pepper Piper spp . ) Extracts of plants,

Lavandula spp .) extracts of plants, and

Mixed extracts of the genus Peppercorn and Lavandula

It relates to a pest control composition comprising at least one selected from the group consisting of as an active ingredient.

The pest control composition is a natural insecticide or repellent composition, and pesticidal activity and / or repelling activity against one or more species selected from the group consisting of moths such as agricultural pests, for example, Chinese cabbage moth, green chestnut moth, tobacco tobacco moth Characterized in having a.

As used herein, 'mixed extract of the plant of the genus Peppera and Lavandula' is an extract of a mixture of the plant of the genus Lavandula and the plant of the genus Lavandula (hereinafter 'extract of the plant mixture') or extract of the plant of the genus Pepper. And a mixture of extracts of plants of the genus Lavandula (hereinafter 'mixture of plant extracts').

In addition, unless otherwise specified in the present specification, 'plant extract' is meant to include crude extracts obtained by extracting the above plants with an extraction solvent, concentrates of the extracts, dried products and plant essential oils obtained from the plants.

The crude extract is extracted from the plant (pepper plant, Lavandula plant or a mixture thereof) with at least one extraction solvent selected from the group consisting of water, linear or branched alcohols having 1 to 4 carbon atoms, chloroform, hexane and the like. It may be obtained by. Specifically, the extractant may be methanol, ethanol, or an aqueous solution thereof, and more specifically 30 to 100% (w / v) methanol or ethanol aqueous solution.

In addition, the crude extract may be obtained in the form of a crude extract itself, or a concentrate obtained by concentrating the crude extract in a conventional manner and dried in a conventional manner. In addition, the plant essential oil may be an essential oil obtained by steam distillation from the plant (pepper plant, Lavandula plant or a mixture thereof).

In one embodiment, pepper crude extract obtained by reflux cooling the pepper, pepper essential oil obtained by treating the pepper by steam distillation method, and / or lavender essential oil obtained by treating the lavandula plant by steam distillation method may be used as an active ingredient. .

Moths, specifically Chinese cabbage moths, green chestnut moths, tobacco germ moths, etc., are pests that damage many crops harvested from plant cultivation or outdoor packaging, causing severe damage to leaves during the larval stage. This is one of the pests that have a lot of resistance to the drug and cause lasting damage.

Thus, the pest control composition of the present invention has an excellent control (insect) effect against moth pests such as Chinese cabbage moth, green chestnut moth, tobacco germweed moth, and among them, insect pests and / or repelling effect to the larvae of extreme damage Is excellent, and more specifically, the insecticidal and / or repellent effect is excellent against the larvae early in development. In addition, the pest control composition of the present invention is not only pesticides and / or even in the same class of moths, such as the moth moth, moth, moth, leaf pattern moth, tent moth, American white moth, etc. It exerts an insecticidal and / or repelling effect on mosquitoes such as mosquito larvae in addition to moths.

Meanwhile, the plant of the genus Pepper used in the present invention is a Piperaceae plant, which has a strong irritation, aroma, and is mainly produced in India, and is cultivated in East India, West India, Brazil, USA, Taiwan, Indonesia, Malay Peninsula, China, etc. do. The amount of irritant contained in the pepper fruit is about 6 to 13% by weight and the amount of pepper essential oil is about 2 to 3% by weight. Pepper fruit has been used to prescribe cholera, indigestion, diarrhea, various gastrointestinal diseases, stroke and arthritis. Pepper species have been widely used for the treatment of fragrances, gastrointestinal tracts, teeth and other diseases, making them a safe plant to be used as a good plant candidate for insecticide development.

Pepper plants that can be used in the present invention is pepper ( Piper nigrum L.), Pepper ( Piper kadzura (Choisy) Ohwi, Pimenta dioica ), Piper betle Piper betel ), Brazilian pepper ( Schinus) terebinthifolius Raddi), white pepper (Piper album L.), etc., the tinnitus of pepper is called reef, cooked, octopus, black pepper (Black pepper). The herbal name is pepper (Piperis Nigri Fructus). Pepper lanterns are known as wind lanterns, rocky scents, and Japanese peppers. The name of the loom pepper is called Chavica Betel, Artanthe Hixagona. Brazilian pepper is called pink pepper or pink pepper. Finally, white pepper is also known as white pepper. In the present invention, the above plant species can be used. Extraction site of the pepper plant may be at least one site selected from the group consisting of whole plants or fruits (seeds), bark, leaves, stems and the like.

Lavandula plant used in the present invention spp .) is an evergreen perennial herb of Lamiaceae. It is native to the Mediterranean coast and is mainly used for herbal tea, aromatherapy and ornamentals. Plants of the genus Lavandula (lavender) are known to have effects such as pain relief, mental stability, sterilization. Lavandula genus plants that can be used in the present invention is Lavandula ( Lavandula) angustifolia Mill.), Lavandula officinalis Chaix, Lavandula vera vera DC.) Or the like may be one or more selected from the group consisting of. The extraction target site of the plant of the genus Lavandula may be one or more sites selected from the group consisting of whole plants or leaves, flowers, stems, seeds, bark and the like.

The plant extract of the present invention is prepared by a conventional plant extraction method, and may be used for extraction by using all or part of dried or undried plants as it is or by grinding. After the extraction, it may be subjected to conventional filtration and / or drying procedures. In addition, the extract may be obtained by additionally separating and purifying by using various conventional chromatographic methods.

Preparation of the plant extract of the present invention may be by any extraction method for extracting the active ingredient from a plant known in the art, for example, steam distillation method using water and / or organic solvent, cold immersion method, warm immersion method, distillation method, One or more methods selected from the group consisting of hot water extraction method, hot water extraction method, reflux extraction method, leaching method, compression method, supercritical gas extraction method, steam distillation method, etc. may be used, but are not limited thereto. For example, when the reflux extraction method is used, extraction may be performed once to five times, for example, twice at a temperature of about 60 to 10 ° C., for example, about 80 ° C. for 2 hours to 4 hours, such as about 3 hours. In addition, in the case of using the cold acupuncture method, 1 to 20 times, for example, about 10 times (volume) of an extraction solvent is administered at room temperature, followed by chilling for about 3 days to extract 1 to 5 times, for example, 2 times. can do.

The pest control composition may include an extract of the plant of the genus of the genus or the extract of the plant of the genus Lavandula as an active ingredient, but is extracted after mixing the plant of the genus Pepper and the plant of Lavandula (extract of the plant mixture) And / or a mixed extract such as a mixture of a plant of the genus Pepper and the extract of the plant of Lavandula (a mixture of plant extracts) as an active ingredient, a marked synergistic effect occurs than when used as a single extract. More preferred (see Experimental Example 2 and Tables 4-6).

When the extract of the plant mixture is used as the mixed extract, the mixing ratio of the plant of the genus Pepper and the plant of Lavandula may be 10: 1 to 1: 1 (pepper plant weight: Lavandula plant weight) by weight. Specifically, 4: 1 to 2: 1. In addition, when a mixture of plant extracts is used as a mixed extract, the mixing ratio of the plant extract of the genus Pepper and the plant extract of the genus Lavandula is 10: 1 to 2: 1 (the weight of the genus Peppermint: Lavandula plant extract) ), Specifically, 5: 1 to 3: 1.

The pest control composition may be used in addition to the active ingredient in combination with conventional insecticides, acaricides and / or fungicides, and may further include excipients or carriers used in these pesticides, sterilization, acaricides. The excipient or carrier may be a conventional material for enhancing ease of use, economy, formulation or insecticidal effect, and examples thereof include flavors, emulsifiers, alcohols and dispersants, but are not limited thereto.

In another example, pest control products (eg, pesticides or repellents) are provided comprising the above pest control composition.

The pest control product may be formulated in a suitable form according to the purpose and use, for example, granules, powders, solutions, aerosols, sprays, extracts, pastes, liquid extracts, emulsions, suspensions, It may be a capsule, a liquid hydrating agent, a granulating hydrating agent, a hydrating powder, a powder, a fine powder, an oil, a gelling agent, a fumigating agent, a fumigating agent, and the like. desirable.

The contents of the plant of the genus P. vanilla and Lavandula as the active ingredient in the pest control product can be appropriately adjusted according to the purpose of use, the product type, the target pest, the target crop, and the like. For example, when each plant extract as an active ingredient is included alone, the concentration of the plant extract of the genus pepper in the product is at least 0.035% (w / w) (such as 0.035 to 90% (w / w)), specifically 0.15% (w / w) or more (eg, 0.15 to 90% (w / w)), more specifically 0.3% (w / w) or more (eg, 0.3 to 90% (w / w)), lavandulas The concentration of the genus plant extract is 0.3% (w / w) or more (eg 0.3 to 90% (w / w)), specifically 1% (w / w) or more (eg 1 to 90% (w / w)) (See Tables 1-3). In addition, when the mixed extract of the plant of the genus Pepper and Lavandula is included as an active ingredient, each plant extract may exhibit excellent control effect even at a lower concentration, so the concentration of the pepper plant extract in the product in the product 0.01% (w / w) or more (e.g. 0.01 to 90% (w / w)), specifically 0.03% (w / w) or more (e.g. 0.03 to 90% (w / w)), lavandulas The concentration of the genus plant extract may be 0.3% (w / w) or more (eg, 0.3 to 90% (w / w)) (see Tables 4 to 6).

When the pest control product includes a mixed extract as an active ingredient, the plant of the genus Pepper and the extracts of the genus Lavandula may be contained in the same container, or contained in a separate container or in a separate container and may be commercialized in a mixed form when used. .

In another example, there is provided a pest control method comprising applying the pest control composition to a crop.

The pest control composition may be diluted to an appropriate concentration during use.

At this time, in the case of using a composition comprising a pepper plant extract as an active ingredient, based on the composition of the pepper plant extract concentration of 30% (w / w), 1 to 800 times by volume, specifically 1 to 200 by volume In addition, even more dilution with 50 to 200 by volume, more specifically 50 to 100 by volume exhibits excellent control effect (see Experimental Example 1 and Tables 1 to 3). Thus, from 1 to about 2500 volume pears, specifically from 1 to about 650 volume pears, more specifically from 50 to about 650 volume pears, more specifically from 50 to about 350, based on the pepper plant extract (100% (w / w)) Diluted by pear can be used.

In addition, in the case of using a composition comprising a plant extract of the genus Lavandula as an active ingredient, excellent control effect even when diluted to 1 to 100 times by volume based on the composition of the Lavandula genus plant extract concentration is 30% (w / w) (See Experimental example 1 and Tables 1-3). Therefore, it can be used diluted to 1 to 350 times by volume based on Lavandula plant extract (100% (w / w)).

In addition, when using a composition comprising a mixture of pepper plant extract and Lavandula plant extract as an active ingredient, pepper plant extract (based on the composition of the pepper plant extract concentration of 30% (w / w)) is Dilution to 1 to 1600 volume fold, specifically 1 to 1000 volume fold, and more specifically 1 to 800 volume fold, shows increased control activity than when used as a single extract (see Experimental Example 2 and Tables 4 to 6). ), Lavandula genus plant extract (based on the composition of Lavandula genus plant concentration is 30% (w / w)) shows a higher control activity than when used as a single extract even when diluted to 1 to 200 vol. Experimental Example 2 and Tables 4 to 6). Therefore, in the case of using a composition comprising a mixture of pepper plant extract and Lavandula plant extract as an active ingredient, pepper plant extract (100% (w / w)) is 1 to about 5000 volume fold, specifically Diluted to 1 to 3500 times by volume, more specifically 1 to 2500 times by volume, and plant extracts of the genus Lavandula (100% (w / w)) is diluted to about 1 to 650 by volume, can be used by mixing them.

In addition, when using a composition comprising an extract of a mixture of the plant of the genus Pepper and the plant of Lavandula (extract concentration 30% (w / w)) as an active ingredient, 1 to 500 volume, specifically 1 to 250 volume Even when diluting to pears, more specifically to 1 to 100 volume pears, shows excellent control activity, the extract (100% (w / w)) of the mixture of pepper and Lavandula plants is 1 to 1600 volume folds, To 1 to 800 volume fold, more specifically, can be used diluted to about 1 to 350 volume fold.

As the dilution solvent, one or more selected from the group consisting of water, alcohols, and excipients may be used, for example, water may be used.

The pest control method may be applicable to a method for spraying or applying the composition for controlling pests directly to crops and / or soil in a facility, or fumigating. In particular, when using fumigation and fumigant, it is appropriate to use the amount of 30 ~ 120 mg per unit area (m ² ), 50 ~ 120 mg per unit space (m ³ ) based on the amount of the active ingredient. However, since its amount is differently applied depending on the growth conditions and size of the crop, it is not limited to the above range.

In addition, the crop applied to the insecticidal action according to the present invention is not particularly limited, and for example, at least one selected from the group consisting of cabbage, lettuce, red pepper, cucumber, soybean, chicory, tomato, eggplant, pumpkin, paprika, melon, melon and the like. Can be.

The pest control composition according to the present invention exhibits excellent insecticidal activity against pests that are severely damaged in plant cultivation or outdoor packaging, and more preferably has excellent pesticidal activity against Chinese cabbage moth, pabam moth, and / or tobacco germ moth. Therefore, it can be usefully used as an effective and environmentally friendly pest control means.

1 is a picture showing the inhibitory effect of Pabam moth feeding on pepper extract, lavender essential oil and a mixture of lavender essential oil and pepper extract.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

[ Example  One]

Preparation of Plant Extracts

Pepper in plant (Piper nigrum ) purchased 10 kg from Paldo ginseng herb concentrate (water source), which specializes in herbal medicine, and crushed finely, and extracted by boiling water twice at 80 ° C for 3 hours using a reflux condenser. At this time, the extraction solvent was used as industrial alcohol (95% (v / v) ethanol), and after extracting the crude powder to the volume of 5 times the volume of the crude powder. After extraction twice, it was filtered using Whatman No. 2 filter paper, and concentrated in a rotary vacuum concentrator (EYELA autojack NAJ-160, Japan) maintained at 45 ° C for the filtrate, and used for the following pesticidal activity experiments. In this manner, 1.2 kg of ethanol crude extract of black pepper was obtained, and the yield was 12%.

Lavandula genus extract was used to purchase lavender essential oil, and 1kg from Hanbit Spice Co., Ltd., which specializes in plant oils, was used for pesticidal activity experiments.

[ Example  2]

Preparation of Single Extract Formulations and Mixed Extract Formulations

(1) Preparation of emulsion composition of pepper crude extract (alone composition)

30 g of the concentrated pepper crude extract prepared in Example 1, 20 g of an emulsifier (castor oil, ethoxylated), and 50 g of ethanol (fermented alcohol, 95% (v / v)) were mixed and stirred at about 200 rpm or more for about 20 minutes. An emulsion composition of pepper extract was prepared. The initial dispersibility of the emulsion was 3DHW / 20 ℃ -30 ℃, 20-300 times, and the emulsion stability was stable for 3DHW / 20 ℃ -30 ℃, 20-300 times / 24hr. Stability for the formulations ranged from -10 ° C to 54 ° C.

The initial dispersibility refers to the degree to which the composition is initially dispersed when diluted with a solvent such as water, and has the same meaning as the hardness of the initial dilution water. As described above, the hardness of water was measured when diluted 20-300 times at a temperature of 20 ° C-30 ° C. The hardness of water was measured using a method of measuring the hardness of water by chelate titration, and the resulting 3DHW means that the Hardness of Water (HW) was 3 degrees (Analytical Chemistry / Jung Ki / Free Academy / 1997 / P. 202, 139, 152, 141, 281-283).

The method of measuring the emulsion stability also measured the hardness of the water, the results show that there is no change in hardness stably after 24 hours after the initial dispersibility measurement.

The formulation stability means the storage temperature of the composition, which means that it can be stored stably without altering the composition when stored at a temperature of -10 ℃ to 54 ℃.

(2) Preparation of an emulsion composition of lavender essential oil (alone composition)

30 g of lavender essential oil prepared in Example 1, 20 g of emulsifier (Castor oil, ethoxylated), and 50 g of ethanol (fermented alcohol, 95% (v / v)) are mixed and stirred at about 200 rpm or more for about 20 minutes to emulsion of lavender essential oil. The composition was prepared. The initial dispersibility for the emulsion was 3DHW / 20 ℃ -30 ℃, 20-300 times, and the emulsion stability showed stability for 3DHW / 20 ℃ -30 ℃, 20-300 times / 24hr. Stability for the formulations ranged from -10 ° C to 54 ° C.

(3) Preparation of Mixed Composition of Black Pepper Extract and Lavender Essential Oil

40 g of the pepper extract prepared in Example 1 and 15 g of an emulsifier (Polyoxyalkylene styrenated phenyl ether; concentrated); weight average molecular weight: 1000; specific gravity: 1.095-1.105; pH 5.0-7.0) were mixed first, and then, in Example 1 10g of the prepared lavender and 35g of ethanol were mixed to prepare a mixed composition of lavender essential oil extract and black pepper crude extract while warming and stirring.

[ Experimental Example ]

Preparation of assay pests for use in experiments

(1) Preparation of Chinese Cabbage Moth Larva

Plutella xylostella is a susceptible strain and was raised in the laboratory with no pesticides, temperature 25 ± 1 ℃, relative humidity 40-60%, and photoperiod 16: 8 (L: D Larvae were bred using young rapeseed seedling under the conditions of), and larvae grown at 2 to 3 years of age were used for the activity assay.

(2) Preparation of the beetle moth larvae

Spodoptera exigua uses a susceptible strain and is a silkworm breeding strain in the laboratory without the need for pesticides. Temperature 25 ± 1 ° C, relative humidity 60-70%, and photoperiod 16: 8 (L: D). Under the conditions of feeding artificial feed (mixed kidney beans, malt powder) were fed to feed, and larvae grown 2 to 3 years old was used for the activity test.

(3) Preparation of tobacco castor larvae

Tobacco giant seminar room (Spodoptera litura (Fabricius) is a susceptible strain, which was raised in the laboratory with no pesticides, and had a temperature of 25 ± 1 ° C, a relative humidity of 60 to 70%, and photoperiod 16: 8 (L: D). Under the conditions, artificial feed (mixed kidney beans and malt powder) was fed and fed, and larvae grown at 2 to 3 years of age were used for the activity assay.

[ Experimental Example  One]

Insecticidal activity of the extract alone composition

Insecticidal activity of the cabbage myth moth of the pepper extract was examined by the above method: 1 ml of the alone emulsion composition (pepper extract concentration 30% (w / w)) of the pepper extract prepared in Example 2 was diluted with water. Diluted 100 to 1600 times by volume ratio (see Table 1) and prepared in a 100 ml beaker with a final treatment concentration of pepper extract in the dilution of 0.3% (v / v) to 0.01875% (v / v). The prepared larvae of Chinese cabbage moths were placed in a clean white gauze, and soaked for 10 seconds in 10 beakers at each dilution concentration in a beaker containing the emulsion composition of the pepper extract prepared above. After that, a 5cm diameter hole was drilled on the top plate, and a fine gauze was put on the hole where the larvae were cut into smaller pieces than the size of the petri dishes. Put it up. The experiment was repeated three times, the progress was observed for 48 hours and the results are shown in Table 1 below. As confirmed in Table 1, the emulsion composition of the pepper extract showed more than 80% insecticidal activity from 100 to 800 times dilution, and showed a tendency to decrease slightly at 1000 and 1600 times dilution.

Insecticidal activity of black pepper extract against pabamung moth and tobacco germweed moth was examined by the following method: 1 ml of the alone emulsion composition (pepper extract concentration 30% (w / w)) of the pepper extract prepared in Example 2 Was diluted 100-fold by volume using water as a diluting solvent, and prepared in a 100 ml beaker so that the final treatment concentration of the pepper extract in the diluent was 0.3% (v / v). About 10 larvae of the prepared beetroot moth was placed in a clean white gauze prepared in a beaker containing the sole emulsion composition of the previously prepared pepper extract was immersed for 30 seconds. After that, a 5cm diameter hole was drilled on the top plate, and a fine gauze was put on the hole where the larvae were cut into smaller pieces than the size of the petri dishes. Put it up. The experiment was repeated three times, and the results were observed for 48 hours, and the results are shown in Table 2 and Table 3 below. As can be seen from Table 2 and Table 3, the emulsion composition of the pepper extract diluted 100 vol. Times (pepper extract concentration: 0.3% (v / v) was 75% and about 41% for Pabam moth and Tobacco coax, respectively). Insecticidal activity was shown.

Chinese cabbage moth insecticidal activity against lavender essential oil was 100 times based on the volume ratio of 200 ml of lavender essential oil emulsion composition (Lavender essential oil concentration 30% (w / w)) as a diluent solvent. Except for the use of pears and dilutions of 400 times, the test was carried out in the same manner as the pesticidal activity test of the pepper extract described above. The results obtained are shown in Table 1. As shown in Table 1, the emulsion composition of lavender essential oil (lavender essential oil concentration 30% (w / w)) was pesticidal activity of 78%, 34%, and 32% at 100-, 200-, and 400-fold dilutions, respectively. Indicated.

Insecticidal activity of green chestnut moth and tobacco germweed moth on lavender essential oil was determined by volume using 1 ml of an emulsion composition (lavender essential oil concentration 30% (w / w)) of lavender essential oil obtained in Example 2 as a diluent solution. The test was carried out in the same manner as the pesticidal activity test of the pepper extract described above, except that a sample having a final lavender essential oil concentration of 0.3% (v / v) was diluted 100-fold. In the experiment, the control group was treated with water, and the experiment was performed in three repetitions. The results obtained are shown in Tables 2 and 3 below. As confirmed in Table 2 and Table 3, lavender essential oil showed insecticidal activity of about 40% and 9.5%, respectively, against the beetroot moth and tobacco germweed moth at a concentration of 0.3% (v / v).

Insecticidal activity of single composition against Chinese cabbage moth (48 hours) Dilution factor / active ingredient concentration Mortality rate (Mean ± SD,%) Lavender essential oil emulsion composition 100 times / 0.3% (v / v) 78 ± 6.78 200 times / 0.15% (v / v) 34 ± 8.99 400 times / 0.075% (v / v) 32 ± 2.36 Emulsion composition of pepper extract 100 times / 0.3% (v / v) 100 ± 0.00 200 times / 0.15% (v / v) 100 ± 0.00 400 times / 0.075% (v / v) 89 ± 5.05 800 times / 0.0375% (v / v) 87 ± 7.04 1,000 times / 0.03% (v / v) 55 ± 7.07 1,600 times / 0.01875% (v / v) 35 ± 7.07 Control group 3.6 ± 5.05

Insecticidal activity of the single composition against pabam moth (48 hours elapsed) Sample (100 times dilution / 0.3% (v / v)) Insecticidal rate (Mean ± SD,%) Emulsion composition of pepper extract 75.0 ± 7.07 Lavender essential oil emulsion composition 40.0 ± 0.00 Control group 0.0 ± 0.00

Insecticidal Activity of the Single Composition against Tobacco Taraxia (48 Hours) Sample (100 times dilution / 0.3% (v / v)) Insecticidal rate (Mean ± SD,%) Emulsion composition of pepper extract 40.8 ± 1.18 Lavender essential oil emulsion composition 9.5 ± 0.64 Control group 0.0 ± 0.00

[ Experimental Example  2]

Insecticidal Activity of the Mixed Composition

Insecticidal activity against the cabbage myth moth of the mixed composition of pepper extract and lavender essential oil was tested by the following method: In the case of lavender essential oil, 200 times the dilution rate of the insecticidal activity against the cabbage myth moth was relatively low in the previous experiment. Pepper extracts were also selected 800 times, 1000 times, and 1600 times dilution ratios (relative volume) was found to be relatively low insecticidal activity. Using a mixture of lavender essential oil and pepper extract of the selected dilution ratio as shown in Table 4 below, the insecticidal activity against the Chinese cabbage moth was tested in the same manner as in Experimental Example 1. The results are shown in Table 4.

Insecticidal activity against cabbage myth moths of lavender essential oil emulsion composition and pepper extract emulsion composition after diluting each concentration sample Final dilution rate (lavender / pepper) Insecticidal rate (Mean ± SD,%) Emulsion composition of lavender essential oil and
Emulsion composition of pepper extract 200 volume / 800 volume 96 ± 5.1 200 volume / 1,000 volume 84 ± 1.9 200 volumes / 1,600 volumes 59 ± 5.1 Control group 0 ± 0.0

As can be seen from Table 4, 50-fold dilution of lavender essential oil emulsion composition (lavender essential oil concentration: 0.3% (v / v)) and 50-fold dilution of emulsion composition of pepper extract (pepper extract concentration: 0.075% ( v / v)) 50 ml of the mixed composition, the active ingredient concentration in the mixed composition is lavender essential oil 0.15% (v / v), pepper extract is 0.0375% (v / v). This mixed composition showed a pesticidal activity of 96% against Chinese cabbage moth. Also, 50 ml of 100-fold dilution of lavender essential oil emulsion composition (lavender essential oil concentration: 0.3% (v / v)) and 50 ml of 500-fold dilution of pepper extract emulsion pepper extract concentration: 0.06% (v / v)) In the mixed composition, the active ingredient concentration in the mixed composition was 0.15% (v / v) of lavender essential oil and 0.03% (v / v) of black pepper composition, which showed 84% insecticidal activity against Chinese cabbage moth. This result is a significantly elevated effect compared to the pesticidal activity of the single mixture corresponding to each concentration. However, a mixed composition of 50 ml of 100-fold dilution (lavender essential oil concentration: 0.3% (v / v)) and 50 ml of 800-fold dilution (pepper extract concentration: 0.0375% (v / v)) of the emulsion composition of pepper extract In the case of about 59% of the insecticidal activity showed a tendency to decrease the effect somewhat. At this time, the active ingredient concentration of the lavender composition is 0.15% (v / v) of lavender essential oil, 0.01875% (v / v) of the pepper composition. As a whole, it was confirmed that the insecticidal activity was 50% or less when treated with each component alone, but showed a significant synergistic effect when they were mixed and treated.

Insecticidal activity assay of green chestnut moth on the mixed composition of the crude crude extract and lavender essential oil prepared in Example 2 (3) was diluted 100 times, 250 times and 500 times by volume ratio with 1 ml of the mixed composition as a diluting solvent. The experiment was repeated three times, and the control group was treated with water to compare the insecticidal activity with the experiment. The progress was observed for 48 hours after treatment. At this time, the active ingredient concentration used in the final dilution is 0.1 times (v / v) lavender essential oil in the case of 100 times dilution, 0.4% (v / v) pepper extract. In the case of 250-fold dilution and 500-fold dilution, lavender essential oil was 0.04% (v / v) and 0.02% (v / v), respectively, and pepper extracts were 0.16% (v / v) and 0.08% (v / v), respectively. to be. The results of the treatment are shown in Table 5 below.

Insecticidal Activity of Mixed Formulations against Pabamoth Moth sample Dilution factor Insecticidal rate (Mean ± SD,%) Lavender and Pepper Mix Formulation 100 × 73.3 ± 0.05 250 × 65.4 ± 0.12 500 × 52.1 ± 0.08 Control group 0.0 ± 0.00

As confirmed in Table 5, the insecticidal activity against the larvae of Pabam moth of the mixture was 73% at 100-fold dilution, 65% at 250-fold dilution, and 52% at 500-fold dilution, respectively.

Insecticidal activity assay of Tobacco Germinate on the mixed composition of the crude crude extract and lavender essential oil prepared in Example 2 (3) is 100 times, 250 times, and 500 times the volume ratio of 1 ml of the mixed composition as a diluent solvent. Three repetitions were performed by diluting, and the control group was treated with water to compare the insecticidal activity with the above experiment. Observation after treatment was observed for 48 hours. At this time, the active ingredient concentration used in the final dilution is 0.1 times (v / v) lavender essential oil in the case of 100 times dilution, 0.4% (v / v) pepper extract. In the case of 250-fold dilution and 500-fold dilution, lavender essential oil was 0.04% (v / v) and 0.02% (v / v), respectively, and pepper extracts were 0.16% (v / v) and 0.08% (v / v), respectively. to be. The results of the treatment are shown in Table 6 below.

Insecticidal Activity of Mixed Formulations on Tobacco Germinants sample Dilution factor Insecticidal rate (Mean ± SD,%) Lavender and Pepper
Mixed formulation 100 × 75.8 ± 0.15 250 × 55.9 ± 0.12 500 × 22.7 ± 0.09 Control group 0.0 ± 0.00

As confirmed in Table 6, the insecticidal activity against the larvae of tobacco germweed moth of the mixture was found to be 75% when diluted 100 times, 55% when diluted 250 times, and 22% when diluted 500 times.

In summary, in the case of Chinese cabbage myth moth, there is some insecticidal activity even when lavender essential oil and pepper extract are used alone, but when the two components are mixed at a concentration of 50% or less insecticidal effect, the synergistic effect is remarkable. It was confirmed to represent. In the case of pabam moth, the pepper extract showed about 75% insecticidal activity at 100-fold dilution when each component was used alone, and it was judged that the pepper extract alone had excellent insecticidal activity. In the case of tobacco spider moth, excellent insecticidal activity was not obtained when pepper extract and lavender essential oil were treated alone, but when they were mixed, it showed a markedly increased insecticidal activity.

[ Experimental Example  3]

feeding  Inhibitory activity test

After spraying about 100 persimmon larvae (2 to 3 years old) to each cabbage, which is a host plant, and spraying about 14 ml of each composition prepared in Example 2 per pot, and then for three days from the time of spraying The degree of Chinese cabbage feeding was observed. The obtained results are shown in Fig. As shown in Figure 1, the feeding was less as confirmed in the emulsion composition of the pepper extract, in contrast, the feeding occurred in the control group treated only with water. In addition, it can be seen that feeding is not very vigorous in the emulsion composition of lavender essential oil compared to the control, and no feeding occurs in the lavender and pepper mixture emulsion composition.

Claims (10)

Pepper Piper spp . ) Extracts of plants,
Lavandula spp .) extracts of plants, and
Mixed extracts of the genus Peppercorn and Lavandula
At least one selected from the group consisting of as an active ingredient;
The mixed extract of the plant of the genus Pepper and the plant of Lavandula is an extract of the mixture of the plant of the genus Lavandula and the mixture of the plant of the genus Lavandula and the extract of the plant of the genus Lavandula;
The extract of the plant of the genus Pepper, the extract of the plant of the genus Lavandula, or the mixture of the mixture of the plant of the genus Lavandula and the plant of the genus Lavandula is from the plant of the genus Pepper, the plant of the genus Lavandula, or the mixture of the plant of the genus Lavandula and
Plant essential oils extracted by steam distillation, and
Crude extract obtained by extraction with at least one solvent selected from the group consisting of water, linear or branched alcohols having 1 to 4 carbon atoms, chloroform, and hexane
At least one selected from the group consisting of,
Pest control composition. The method of claim 1,
The Piper spp . ) Pepper plant (Piper nigrum L.), Piper kadzura (Choisy) Ohwi, Pimenta dioica ), Piper betle Piper betel ), Brazilian pepper ( Schinus terebinthifolius Raddi), and one or more plants selected from the group consisting of White pepper ( Piper album L.),
Lavandula spp .) The plant is Lavandula angustifolia Mill.), Lavandula officinalis Chaix), and at least one plant selected from the group consisting of Lavandula vera DC.,
Pest control composition. The method of claim 1,
The extract of the mixture of the plant of the genus Lavandula and the plant of the genus Lavandula is a mixture of the plant and the genus Lavandula in a ratio of 10: 1 to 1: 1 (pepper plant weight: Lavandula plant weight) by weight An extract obtained from the mixture,
The mixture of the extract of the plant of the genus of the genus Lavandula and the extract of the plant of the genus Lavandula is 10: 1 to 2: 1 by weight of the extract of the plant of the genus Lavandula and the extract of the genus Lavandula Mixed with the ratio of the extract weight of the plant),
Pest control composition. 4. The method according to any one of claims 1 to 3,
The pest control composition exhibits pesticidal activity to at least one selected from the group consisting of Chinese cabbage moth, pabam moth, and tobacco germ moth,
Pest control composition. A pesticide product comprising the composition for controlling pests according to any one of claims 1 to 3. The method of claim 5,
The insecticide product is to show insecticidal activity to at least one selected from the group consisting of Chinese cabbage moth, pabam moth, and tobacco germ moth,
Insecticide products. The method of claim 5,
The pesticide products include granules, powders, liquids, aerosols, sprays, extracts, pastes, liquid extracts, emulsions, suspensions, capsules, liquid hydrating agents, granulating hydrating agents, hydrating agents, powders, granules, oils, Formulated in gel, smoke, or fumigant,
Insecticide products. A pest control method comprising the step of applying a composition for controlling pests of any one of claims 1 to 3 to the crop. 9. The method of claim 8,
The pest is at least one selected from the group consisting of Chinese cabbage moth, pabam moth, tobacco tobacco moth, pest control method. 9. The method of claim 8,
The target crop is at least one selected from the group consisting of cabbage, lettuce, pepper, cucumber, soybean, chicory, tomato, eggplant, pumpkin, paprika, melon, and melon,
Pest control method.

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