KR100852398B1 - Composition for controlling noxious animals in horticultural crops and using method thereof - Google Patents

Abstract

A composition for controlling noxious animals in horticultural crops is provided to inhibit resistance against agricultural chemicals, and harmfulness and residual toxicity to human and domestic animals, and ensure post harvesting safety, so that the composition is applicable to the horticultural crops during the harvesting time. A composition for controlling noxious animals including snail, earthworm and leech in horticultural crops contains (a) 100 parts by weight of the extracts of a mixture containing at least one tree selected from Thea sinensis and Camellis japonica, and at least one selected from Azadirachta indica, Allium scorodorpasum var. viviparum Regel(garlic) and Zingiber officinale(ginger), which are prepared by extracting the mixture with water, C1-C4 alcohol, ethylacetate or hexane, and (b) 10-200 parts by weight of at least one compound selected from saponin and catechin. Further, the 35-65 parts by weight of saponin and the 10-30 parts by weight of catechin are comprised in the10-200 parts by weight of at least one compound.

Description

Composition for controlling noxious plants containing plant extracts and method for controlling phytotoxic animals using the same {composition for controlling noxious animals in horticultural crops and using method}

1 is a photograph showing the control effect of a snail treated with a composition according to an embodiment of the present invention, the left photograph shows an untreated sphere, the right photograph shows a composition treatment sphere of the present invention diluted 100 times the composition of the present invention. .

Figure 2 is a photograph showing the control effect of the earthworm treated with a composition according to an embodiment of the present invention, the left photograph shows an untreated sphere, the right photograph shows a composition treatment sphere of the present invention diluted 100 times the composition of the present invention. .

Figure 3 is a photograph showing the control effect of the leech treated with the composition according to an embodiment of the present invention, the left photograph shows an untreated sphere, the right photograph shows a composition treatment sphere of the present invention diluted 100 times the composition of the present invention. .

The present invention relates to a control composition of a plant harmful animal and a method of using the same to control harmful animals such as snails, earthworms and leeches, which are problematic when horticultural crops are grown.

Most of the snails that cause various horticultural crops are caused by silk snails and slugs. Snails are one of the major harmful animals that lower productivity and marketability. Up to now, snail control has been used a variety of synthetic insecticides, but the excessive use of synthetic insecticides as well as the generation of resistant harmful animals, as well as environmental hazards and residual toxicity has been pointed out as a social problem. Recently, the demand for eco-friendly agricultural products, which guarantees the safety of agricultural products, is rapidly increasing. Therefore, researches are being actively conducted to control harmful animals (snails, earthworms, leeches) without using synthetic pesticides in order to meet the social demands for environmental conservation. have.

Korean Patent Registration No. 10-0638266 is a mixture of extracts such as Goryun, red ginseng, branch vein, night country, camellia, Jinrang, Sagunja, white paper, and Yeoro, and the plant extract concentrated mixture is prepared from rice bran, Chinese cabbage moth, spotted mite, tobacco spider Techniques for use as insecticides of moths, leafworms, oyster flies, greenhouse dusts, blue worms, green chestnut moths, carob moths, slugs, aphids, rice weevils, raspberry moths, or moths moths are disclosed.

In Korean Patent Publication No. 2005-0115521, etc., various insecticidal plant extract compositions prepared by combining one or two or more of mulberry extract (Nim oil), baekeuneun extract, cypress extract, baekyang extract, castor extract There are reports of various pest control effects using.

Research into biological pesticides such as natural pesticides and natural microorganisms to replace the chemical pesticides, but most natural pesticides and natural microorganisms have a weak control effect or extremely limited effect, and the price is also expensive Since it is not effective and economically unfavorable to farmers, it has not succeeded as a substitute for chemical pesticides to realize eco-friendly agriculture.

It is an object of the present invention to provide a pest control composition of horticultural crops and a method of using the same, which are harmless to the environment and human beings, and can guarantee the safety of agricultural products while effectively controlling the pests of horticultural crops.

Still another object of the present invention is a pest control composition of a horticultural crop which can produce safe agricultural products as a safe control agent that can replace chemical pesticides by controlling the pests of plants to a level similar to that of spraying synthetic insecticides, and It provides a way to use it.

Another object of the present invention is to control harmful animals even if the mixed treatment with conventional chemical pesticides or spraying in the middle of the treatment of chemical pesticides can be harmful pest control composition of horticultural crops that can contribute to reducing the number of spraying chemical pesticides and use thereof To provide a way.

In order to achieve the above technical problem, the present invention is a) one or more plant extracts selected from the group consisting of tea, camellia, mulberry, garlic, and ginger; And

b) provides a composition for controlling harmful animals of horticultural crops comprising at least one compound selected from the group consisting of caffeine, saponins and catechins. Preferably the pest is a snail, earthworm or leech. The plant extract is selected from the group consisting of water, alcohol having 1 to 4 carbon atoms, ethyl acetate, hexane, and mixtures thereof, at least one plant selected from the group consisting of tea tree, camellia, mulberry, garlic, and ginger. The solvent extraction may be one or more solvents.

The present invention also includes diluting the horticultural crop harmful animal composition to 10 times (volume ratio) to 500 times (volume ratio), preferably 50 times (volume ratio) to 500 times (volume ratio) and spraying the harmful animals. To provide a method of controlling harmful animals to horticultural crops. Preferably, the spacing of the horticultural crops after spraying 3 to 4 times with a treatment interval of 3 to 7 Repeated spraying periodically.

Horticultural crops of the present invention (snail, earthworm, leech) When spraying the composition for controlling the foliage on the mollusk snail, earthworm. It strongly stimulates the skin of the body surface such as leech, and the biological protection function is sharply lowered. The insecticidal mechanism shows insecticidal effect, repellent effect, and feeding inhibition effect on snails, earthworms and leeches.

The main component of the composition for controlling pests of horticultural crops according to the present invention is caffeine (alkanoid), saponin (terpenoid), and catechin (polyphenol) which are secondary metabolites of plants. It is suitable, and there is no harmful and no residual toxicity to humans and livestock, so the safety of agricultural products is guaranteed, so it is possible to process during the harvest season of horticultural crops. When spraying these compositions, the harmful effects of horticultural crops (snail, earthworm, leech) are effective for up to 7 days after spraying, and they are safe for crops and can control snails. It is a substance. In addition, it is possible to control harmful pesticides (snails, earthworms, leeches) even if mixed with chemical pesticides or spraying in the middle of the treatment can greatly contribute to reducing the frequency of spraying chemical pesticides.

Hereinafter, the present invention will be described in more detail.

The components of the composition for controlling horticultural crops according to the present invention are a) plant extracts and b) alkanoids, terpenoids, or polyphenols which are secondary metabolites of plants. Examples of the secondary metabolite include caffeine as alkanoids, saponin as terpenoids, and catechin as polyphenols.

In the case of caffeine (CAS No. 58-08-2), the US Department of Agricultural Research (ARS) Hollingsworth's team announced that they killed nearly all snails and slugs in two days with 1-2% caffeine solution (Hollingsworth, RG, Armstrong, JW & Campbell, E. Caffeine as a repellent for slugs and snails.Nature, 417, 915-916, 2002). Catechin (catechin) is a unit that forms a condensation of the tannin component of tea is a flavonoid that combines with various vegetable oil components and acts as a burst to rupture worms such as linear and cyclic animals. In addition, steroids saponin (steroids saponin) is known as a terpenoid-based material to increase the resistance to various pests.

The mixing ratio of the a) plant extract and the b) compound in the composition for controlling the present invention may be 10 to 200 parts by weight of the compound based on 100 parts by weight of the plant extract. In addition, the content of the compound may be selected from the group consisting of caffeine, saponin, and catechin, the content of each compound, 20 parts by weight or more of caffeine, preferably 20 parts by weight based on 100 parts by weight of the plant extract Parts to 200 parts by weight, more preferably 20 to 40 parts by weight, saponin is 35 parts by weight or more, preferably 35 parts by weight to 200 parts by weight, more preferably 35 to 65 parts by weight, and catechin is 10 parts by weight or more , Preferably from 10 parts by weight to 200 parts by weight, more preferably from 10 to 30 parts by weight.

The plant extract may be at least one plant selected from the group consisting of tea tree, camellia, mulberry, garlic, and ginger, and preferably comprises at least one selected from the group consisting of tea tree and camellia. It may be good, and optionally, may further contain one or more selected from the group consisting of mulberry, garlic and ginger. Most preferably, all five of these plants are included. The plant includes all of the leaves, branches, roots, etc., Preferably, the tea, camellia, and mulberry tree is preferably to use the leaves, ginger is the diameter, garlic is good to use the diameter.

Thea sinensis is an evergreen tree belonging to the tea tree family of the dicotyledonous plant with the location of plant classification. Most of the green leaves of tea are occupied by water (75 to 80%), but in solids the soluble component is less than 50%. Soluble ingredients are water-soluble substances and the tannins of tea that give us flavor are polyphenols (catechins), amino acids, caffeine, and sugars. Among them, polyphenols are called 10 to 18% of tea tannin or catechin. 50% fiber, 15% protein (including enzymes), 7% carbohydrate (3.2-6.4% pectin), 0.9-2.3% sugar, 0.3-0.8% glucose + fructose, 7% lipid, 1.6-1.6 free amino acids and peptides 5%, 2-4% caffeine, 5% minerals and 0.5% organic acids, including other nucleic acids, saponins, fragrance components, and the like.

Camellia japonica , like the Camellia japonica , is an evergreen tree belonging to the Tea Tree family of the dicotyledonous plant. It mainly uses undried oil squeezed from seeds for food.

Mulberry ( Azadirachta indica ) is a deciduous broad-leaved arboreous tree, with fruit as (spring lotus), 葉 (leaf) as (leaves), flower as (flowers), softened, nets (roots) and 樹皮 (bark) as It is called medicinal. The medicinal herb is dried in the sun or in the sun after drying in the autumn. The ingredient contains Toosendanin, which is an active ingredient of a roundworm. The leaves are harvested in summer and autumn and dried in the sun. The leaves contain quercitrin and rutin. Softening is a flower and contains flavonoid glycosides myricetin-3-l-arabinoside, quercetin-3-galactoside and astragalin, and it is sprayed with softening on the sweat band (Hanjin). It can be collected from year to year, but it is good from late spring and early summer, and it is dried in the sun by cutting the stems, cutting the roots and peeling them, and it contains triterpenoids.

The special ingredients contained in garlic ( Allium scorodorpasum var.viviparum Regel ) are known to have strong bactericidal and tonic effects as irritants such as diallyl sulfide and allyl propyll sulfide. Like this, garlic is used as a spice due to its peculiar smell. This is because alliin, a type of sulfur amino acid, is decomposed by allinase enzyme and becomes allicin.

Ginger ( Zingiber officinale ) is a very important vegetable and is widely used in edible and medicinal industries around the world. Its use is for reproduction, pickling, medicinal and industrial purposes. The caliber contains 80.8% moisture, 2.3% protein, 1.0% fat, 12.3% carbohydrates, 2.4% fiber, and 1.2% ash. Raw meat is mainly used for various kinds of pickling materials, including root and leaf ginger, and pickling for salting. Dried ginger is widely used for food, medicinal and industrial purposes. Ginger's unique fragrance and spicy taste are important for appetite and toxic neutralization.

The above plant extract mixtures have a wide range of insecticidal, repelling and feeding-inhibiting effects. Secondary metabolites of plants are widely used in various industries due to their types and functions. In particular, the functions of secondary metabolites such as catechins, polyphenols, saponins, terpenoids, and caffeine, alkanoids, can effectively control various pests as well as snails, earthworms and leeches, which are harmful to agricultural land. It can be developed as a biopesticide.

The plant extract of the present invention comprises at least one plant selected from the group consisting of tea tree, camellia, mulberry, garlic, and ginger in the group consisting of water, alcohol having 1 to 4 carbon atoms, ethyl acetate, hexane and mixtures thereof. The solvent extraction may be one or more solvents selected. The solvent-extracted plant extract is 10 to 20 parts by weight of tea tree and / or 10 to 20 parts by weight of camellia, or additionally 3 to 10 parts by weight of mulberry and / or 5 to 15 parts by weight of garlic and / or ginger Solvent extraction may be 3 to 10 parts by weight. In the plant extract of the present invention, when two or more kinds of plant extracts are mixed, extracts obtained by extracting these plants, respectively, may be mixed, or a mixed extract obtained by extracting these plants together may be used.

The extraction solvent may be more preferably 50 to 99% ethanol dilution water or methanol dilution water, and most preferably 50 to 99% ethanol dilution water.

Plant extract of the present invention may be prepared according to a conventional method for producing a plant extract, for example, but may be by cold extraction, hot extraction, heat extraction or reflux extraction method, but is not limited thereto. When the extraction solvent is water, it may be by hot water extraction, and when the extraction solvent is alcohol, it may be by reflux extraction. In addition, the extract extracted with the solvent may further perform a fractionation process with a solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, chloroform and mixtures thereof after performing the extraction process, the solvent during the fractionation Can be used one or more. The extract preparation temperature may be 4 to 120 ℃, but is not limited to this, the extraction time is not particularly limited, but may be 10 minutes to 300 minutes, but is not limited thereto. The extraction process may be performed using a conventional extraction device, an ultrasonic mill extractor or a fractionator, but is not limited thereto. The prepared extract may then be concentrated by filtration under reduced pressure or drying to remove the solvent, filtration and drying under reduced pressure, and the filtration under reduced pressure may be carried out by low pressure vacuum concentration using a vacuum concentrator. It may be performed by a lyophilization method, but is not limited thereto.

In one specific embodiment of the present invention, the plant extract may be prepared by extracting the plants under reflux by adding ethanol dilution water, filtering the obtained extract using filter paper, concentrating by vacuum reduction, and freeze-drying.

In another embodiment of the present invention, the composition for controlling horticultural crops according to the present invention is diluted by 10 times (volume ratio) to 500 times (volume ratio), preferably 50 times (volume ratio) to 500 times (volume ratio). Provided are methods for controlling horticultural crops, including spraying on harmful animals.

3 to 4 times the spacing after the application to the foliage of the horticultural crops 3 to 7 days Periodic repeated spraying is preferred. As a specific example, plant extracts including tea, camellia, mulberry, garlic, and ginger extracts were prepared as a stock solution, 50%, catechin 10%, caffeine 15%, and saponin 25%, diluted 100-fold, and then diluted at 5-day intervals. Spraying leaves 3-4 times late in the afternoon or early morning without sunlight.

Spraying the present invention on the foliage of horticultural crops being cultivated periodically can reduce the use of synthetic insecticides and produce safer agricultural products by controlling harmful animals (snails, earthworms, leeches) to a level similar to that of spraying synthetic insecticides.

Horticultural crops of the present invention (snail, earthworm, leech) When spraying the composition for controlling the foliage on the mollusk snail, earthworm. It strongly stimulates the skin of the body surface such as leech, and the biological protection function is sharply lowered. The insecticidal mechanism shows insecticidal effect, repellent effect, and feeding inhibition effect on snails, earthworms and leeches.

The present invention is based on the expression of insecticidal, repellent and feeding inhibitory effects by various plant extract mixtures, caffeine (alkanoid), which mainly exhibits eating inhibitory effects, and saponins (terpenoids) that facilitate the adsorption to the skin of plants and pests. And catechins (polyphenols), which have repelling and insecticidal effects, are composed of secondary metabolites. Horticultural crops Harmful animals (snails, earthworms, leeches) When spraying the control composition on the foliage, they come into contact with and penetrate the body surface of snails, earthworms, leeches, etc. , Leeches.

The present invention allows commercially available products to be formulated, preferably as concentrates, and end-users can typically use dilute formulations. The compositions according to the present invention are all applied to the plants by themselves and applied to essential plants and It may be advantageously formulated into flowable compositions, suspensions, microsuspensions, suspoemulsions, wettable powders, granule concentrates, microemulsions and the like, which provide.

Such control compositions generally comprise 0.1 to 99%, in particular 10 to 75%, more particularly 20 to 60%, of the active substance, with the remainder of the formulation being any surfactant (s) and any other known in the art. Solid and / or liquid carriers with inert ingredients such as protective colloids, adhesives, thickeners, thixotropic agents, penetrants, preservatives, stabilizers, antifoams, cryoprotectants, metal ion sequestrants, dyes, pigments, colorants and polymers (Eg, water). In addition, the compositions of the present invention may further comprise a carrier of natural or synthetic, organic or inorganic material which is further combined with the active material to facilitate plant, seed or soil application.

For the purpose of illustrating the present invention in more detail with reference to the following examples, the protection scope of the present invention is not intended to be limited to the following examples.

Example 1. Preparation of Control Composition

1-1: Preparation of Plant Extract

The preparation method of the plant extract and the final composition according to the present invention is as follows:

1) As a material used for the plant extract of the present invention, tea leaves, camellia leaves, mulberry leaves, garlic garlic, ginger diameter were put in a drier and dried at 70 ° C. for 48 hours.

2) Each dried sample was cut into 1 cm or less using a power cutter.

3) The pulverized tea tree, camellia, mulberry, garlic and ginger were mixed at the mixing ratios shown in Table 1 below, respectively.

4) 1 kg of the mixture was immersed in 2 L of ethanol, and extracted at room temperature for 48 hours.

5) The extract was filtered and concentrated under reduced pressure to prepare a concentrate by adjusting the filtrate to 10 to 15% by weight.

1-2: Preparation of Control Composition

Several final mixed compositions were prepared by mixing caffeine, saponins, and catechins in a weight ratio as shown in Table 1 below in a plant extract concentrate obtained in Example 1-2 in a weight ratio.

The pest control effect of horticultural crops is more effective by adding caffeine, saponin, catechin and the like to the plant extract concentrate.

Therefore, as shown in Table 1 below, the stock solution is formulated and packaged to prepare a stock solution composition for controlling harmful animals (snails, earthworms, leeches) composed of the present invention, when spraying the mixed composition and dilution factor in consideration of the control effect. Spraying in consideration of effective control of pests of horticultural crops. Most preferably, it is diluted 100-fold with the combination of sample 7 and spread the leaves to the leaves 3-4 times a sufficient amount every 7 days. Water was used as the extender for final content control.

[Table 1] Composition for the control containing five plant extracts

[Table 2] Composition for the control containing three plant extracts

[Table 3] Composition for control containing two plant extracts

Example  2. Control of harmful animals by treatment composition

In order to test the harmful control effect of the 77 mixed compositions prepared in Example 1-2, each composition was diluted 100, 500, 1,000 times, and the basic test was carried out by spraying a sufficient amount so that the target animal contacted.

Snails, earthworms and leeches were collected from cabbage fields, lawns and buttercup fields, respectively, and then purified for 2 days in indoor breeding. 20 animals were used. Snails and earthworms were sprayed to make direct contact. It was sprayed by the method. The results of the investigation of the control effect at 5 days after spraying are shown in Tables 4 to 6 below.

[Table 4] Control Effect of Five Plant Extract-Containing Compositions

TABLE 5

TABLE 6

As can be seen in Tables 4 to 6, in the case of tea and camellia extracts, when these two components are mixed together, or when each of them or a mixture of these mixtures with other components was shown to exhibit excellent control effect. In addition, in the case of mulberry, garlic, ginger extract showed a characteristic that the control effect is increased when used as a mixed composition mixed with tea or camellia extract rather than a single treatment. Therefore, in order to effectively control harmful animals such as snails, earthworms, leeches and the like, at least one of the tea extract and camellia extract is preferably treated with a plant extract mixed composition comprising at least 15% by weight each. have.

In addition, as a result of analyzing the combination of the mixture (samples 1 to 7) and the compound containing all five plant extracts, the control effect was the best when all three species (saponin, catechin, caffeine) were mixed In the case of two species, saponin + catechin> caffeine + saponin> caffeine + catechin was found in order, and in one case, saponin> catechin> saponin. The best combination was the test treated with 100 times the caffeine, saponin, and catechin all added to the five mixtures. Overall, over 80% of all treatments were rated for practical control.

In addition, as can be seen from the comparison of Samples 29 to 35 with other samples, it was found that the composition containing the tea and / or camellia extract showed a superior control effect than the composition not containing it.

Example 3 Control Effect of Snail by Composition Treatment

The occurrence of snails (silk snails, slugs) during the growth of Chinese cabbage is very fatal. After diluting the sample 7 composition of Example 1-2 to 100 times in the early or developing snail period, the level is 150-200 ml per m 2. When sprayed so that the snails are in contact with the silk snail and slug as shown in Figure 1 excellent control.

Figure 1 shows the control effect of the snail 3 days after the treatment of the sample 7 of Example 1, the left picture shows the untreated tool, the right picture shows a 100-fold dilution treatment.

Example 4. Effect of snail control over time by composition treatment

In the actual packaging test, the control effect on the snail of Sample 7 of Example 1-2, which was the most effective among the mixed compositions, was examined. Chinese cabbage was prepared in open field, and diluted 100-fold dilutions of the composition for controlling harmful animals once, twice, and three times at 60-day intervals were applied to the leaves. The control group was treated with untreated and synthetic insecticides, and the synthetic insecticides were treated with namethox granules of metaaldehyde components having a manned insecticidal mechanism in the soil. The insecticidal effect by the control composition and the decoy insecticide effect by the synthetic insecticide was analyzed. On the 5th day after the single spraying, the control effect of 91.3% and 90.7% of the synthetic insecticide treatment and the treatment of the composition was 87.7% and 81.7%, respectively. It was. On the 15th day, 80.0% and 67.3% of the synthetic insecticide treatments and the composition treatments were effective.

On the 5th day after 2 times of spraying every 5 days, the control effect was 94.7% and 93.0% in the synthetic insecticide treatment and the composition treatment, respectively. On the 10th day, the control value was 92.3% in the synthetic insecticide treatment. 87.7%. On the 15th day, the control value was 83.3% in the synthetic insecticide treatment group and 71.0% in the composition treatment treatment group.

On the 5th day after 3 times of spraying every 5 days, the control effect was 97.3% and 95.0% in the synthetic insecticide treatment and the composition treatment, respectively. In the 10th day, 95.3% and 92.3% in the synthetic insecticide treatment and the treatment It was. On the 15th day, the control effect of the synthetic insecticide treatment and the composition treatment showed 86.0% and 79.7% control effect, respectively.

The results obtained above are summarized in Table 7 below.

[Table 7] Comparison of snail control effect over time by the composition treatment

Control value = (occurrence rate of untreated group-incidence rate of treated group) / incidence rate of untreated group] X 100

Example 5 Control Effect of Earthworms by Composition Treatment

Earthworms that occur on golf courses are a major cause of increased golfers' dissatisfaction and aversion. After diluting the sample 7 composition of Example 1 by 100 times at the beginning or the generation of earthworms, the earthworms were excellently controlled as shown in FIG. In particular, lawn worms are not exposed to the outside, so effective treatment can be achieved by adhering to the treatment volume. Figure 2 is a photograph showing the control effect of the earthworm three days after the treatment of the sample 7 composition of Example 1, the left side is an untreated sphere, the right side is a 100-fold dilution treatment.

Example 6 Earthworm Control Effect Over Time by Composition Treatment

In the actual packaging test, the control effect on the earthworm of the sample 7 composition of Example 1-2, which was the most effective among the mixed compositions, was examined. In order to effectively control the grassworms, the amount of the treated composition is an important requirement. The grass to be tested is Western grass. In this test, the amount of treated water was 2,000 ml per 1 m 2, and the spray was applied so that the stock solution of the composition for control was diluted 100-fold. The control group was treated with untreated and synthetic insecticides, and the synthetic insecticides were treated with soil cap mocap, a soil pesticide. The insecticidal effect of the composition for control and synthetic insecticide was investigated and analyzed. In the case of the synthetic insecticide treatment, all of the treatments died at 5, 10, and 15 days after treatment. The control composition showed 95.0, 92.3, and 88.3% control effects in the 5th, 10th, and 15th days after the single spray, respectively, and the 5th, 10th, and 15th days after the 2 sprays every 5 days. 96.7, 95.0, 93.0% showed control effect. After 5 times, 10 days, and 15 days after spraying every 5 days, 97.3, 95.7 and 94.3% of the control were found, respectively. .

The results obtained above are summarized in Table 8 below.

Table 8 Comparison of Earthworm Control Effects Over Time by Composition Treatment

Control value = (occurrence rate of untreated section-incidence of treated section) / incidence rate of untreated section] X 100

Example 7 Control Effect of Leech by Composition Treatment

Buttercup, grown under freshwater conditions, is severely damaged by aquatic organisms such as leeches and snails. In particular, leeches are strongly adhered to crops and the damage is severe. Since leeches inhabit fresh water conditions, the freshwater depth is adjusted to 1 cm in a 1m2 area so that leeches in the buttercup field having a quantity of 1 kg (1 L) may be 100 times the sample 7 composition of Example 1 above. 10 g was weighed on the m 2 area and sprayed evenly on the surface of the water to control the leeches as shown in FIG. 3. Figure 3 is a photograph showing the control effect of leeches on the third day after the composition treatment, the left side is untreated and the right side is 100 times diluted treatment.

Example 8 Effect of Leech Control over Time by Composition Treatment

The fresh water level of the hydroponic cultivated buttercup field grown about 60 cm long was sprayed so that the stock solution of the composition 7 for control of Example 7 was diluted 100-fold once, twice, and three times at 5-day intervals. The control effect on leeches was examined using the untreated group as a control.

On the 5th day after the single spray, the composition treatment showed 73.7% control, and on the 10th and 15th days, 75.0% control and 70.3% control, respectively. In the case of 5 days after two sprays every 5 days, the composition treatment showed 92.7% control, and on day 10 and 15, 85.3% control and 79.7% control, respectively. On the other hand, after 5 times of spraying every 5 days, 95.0% control value was shown in the composition treatment, and on day 10 and 15, 90.3% control and 84.7% respectively. Therefore, leech was effective in proportion to the number of treatment of the composition for the control, and about 15 days after treatment showed a tendency to slightly reduce the control effect in the pretreatment.

The results obtained above are summarized in Table 9 below.

Table 9 Comparison of the leech control effect over time by the composition treatment

Control value = (occurrence rate of untreated section-incidence rate of treated section) / incidence rate of untreated section] X 100

Example 9 Safety of Various Crops by Treatment of Compositions

The composition for the control should be excellent to control the harmful animals such as snails, earthworms, leeches and at the same time must ensure the safety of the crop. Therefore, in order to examine the effect on various crops, the composition 7 for the control composition of Sample 7 of Example 1-2 was diluted and sprayed to 100 times and 50 times. After 1 month, 3 days, 5 days, and 10 days after the application of the crown tube, as shown in Table 10, the composition is very safe for crops, so it is suitable for use in environmentally friendly crop cultivation.

[Table 10] Comparison of crop safety by treatment

* Degree of damage: Moon valuation (0; no damage, 1; 1-10% damage, 2; 11-30% damage, 3; 31-50% damage, 4; 51-80% damage, 5; 81% or more) damage)

Example 10. Relative Mobility Comparison of Snails, Earthworms and Leeches by Composition Treatment

It is possible to effectively control harmful animals such as snails, earthworms, leeches, etc., which are targeted by the control composition, by causing intense skin irritation. Earthworms, snails, and leeches have many differences, such as damage crops, habitats, and animal classifications. Is known to have a wide range of effects despite some differences between pests. Therefore, in order to find out the skin irritation effect on the snails, earthworms, leeches that are harmful animals, the sample solution of Example 7 for the control of Example 1 was diluted and sprayed to 100 times and 50 times.

At 10, 20, 30, and 60 minutes after spraying, the relative motility value (RM value) was evaluated by comparison with the untreated group, respectively. As a result, the snails began to show vigorous motility at 10 minutes of treatment in both 50- and 100-fold treatments, reaching a peak at 20 minutes. After that, the skin stimulation started to kill at 30 minutes due to damage and the mobility became extremely dull. At 60 minutes, all were killed to show excellent control effect. In the case of earthworms and leeches, the relative kinetic response patterns with concentration and time-lapse were very similar. Earthworms were more sensitive to skin irritation because of their faster motility than snails and leeches, and leeches showed rapid movement over time, resulting in longer locomotion than snails and earthworms, but at about 90% of 60 minutes Control effect was shown.

The results are summarized in Table 11 below.

TABLE 11 Relative Mobility Comparison of Snails, Earthworms and Leeches by Composition Treatment

* The RM value represents the relative motility value, and the degree of motility is expressed as large; +++, medium; ++, small; +, no;-. (Minor motility of the untreated area is considered "no".)

Caffeine (alkanoid), saponin (terpenoid), and catechin (polyphenol), which are the main components of the present invention, can be easily purchased and can be manufactured even in farms by a simple process of dissolving in water at a relatively low concentration. There is no problem of resistance when spraying synthetic synthetic insecticides for controlling harmful animals (snails, earthworms, leeches) of horticultural crops, and it is possible to solve the problems of harmfulness and residual toxicity to humans and livestock, which ensures safety after harvesting. The horticultural crop can also be processed during the harvest season.

Claims (10)

a) at least one plant selected from the group consisting of tea and camellia, and at least one plant mixture extract selected from the group consisting of mulberry, garlic and ginger; And b) saponins, and at least one compound selected from the group consisting of catechins Composition for controlling harmful animals of horticultural crops. The composition of claim 1, comprising 10 to 200 parts by weight of compound b) based on 100 parts by weight of the plant extract a). The composition of claim 1, wherein the pest of the horticultural crop is one or more selected from the group consisting of snails, earthworms, and leeches. The composition of claim 1, wherein the plant extract a) is solvent extracted using at least one selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate, and hexane. The composition of claim 1, wherein the compound b) comprises 35 to 65 parts by weight of saponin and 10 to 30 parts by weight of catechin, based on 100 parts by weight of the plant extract a). According to claim 2, wherein the plant extract a) is 10 to 20 parts by weight of tea tree, 10 to 20 parts by weight of camellia, 3 to 10 parts by weight of mulberry tree, 5 to 15 parts by weight of garlic, and 3 to 10 parts by weight of ginger A composition for controlling horticultural crops, wherein the mixture is solvent extracted. A method for controlling horticultural crops, comprising diluting the composition for controlling horticultural crops according to any one of claims 1 to 6 to 10 times (volume ratio) to 500 times (volume ratio) and spraying the harmful animals. Way. The method of controlling a horticultural crop noxious animal according to claim 7, wherein the horticultural crop is sprayed on the foliage of the horticultural crop three to four times periodically after the treatment interval is 3 to 7 days. According to any one of claims 1 to 6, wherein the b) the compound further comprises caffeine, the composition for controlling horticultural crop pests comprising caffeine in 20 to 40 parts by weight based on 100 parts by weight of the plant extract a). A method for controlling a horticultural crop harmful animal, comprising diluting the composition for controlling a horticultural crop harmful animal according to claim 9 to 10 times (volume ratio) to 500 times (volume ratio).

Images