KR102540101B1 - Control compositions for crop pests capable of water and soil treatment And Methods for preparing the same - Google Patents

Abstract

The present invention is effective in controlling crop pests that can be treated with water and soil, and is effective in mass production. Water surface treatment and soil treatment for insect pest control, which are formulated in a certain form by mixing tea tree extract and neem extract with sulfur powder, diatomaceous earth powder, and auxiliary agents, in a composition containing two or more main components such as camphor oil and cinnamon oil as raw materials. It relates to a control composition for possible crop pests and a manufacturing method thereof.

Description

Control compositions for crop pests capable of water and soil treatment And Methods for preparing the same}

The present invention relates to a control composition for crop pests capable of water treatment and soil treatment and a method for producing the same, and more particularly, to a control composition for crop pests capable of water treatment and soil treatment, effective for controlling crop pests capable of water treatment and soil treatment, and neem extract capable of mass production, In a composition containing two or more main components, such as plant extracts (mulberry, yangmyeong aju, fig tree, clove, tea tree extract, etc.), sulfur, deris extract, camphor tree oil, cinnamon oil, tea tree extract and neem extract, sulfur powder, diatomaceous earth powder And it relates to a control composition for crop pests capable of water surface treatment and soil treatment for insect pest management, which is formulated and formulated into a certain form by mixing an auxiliary, and a method of manufacturing the same.

Various diseases and pests occurring in crops not only directly reduce yield, but also adversely affect quality. In order to prevent these pests, the development and use of new pesticides have been continuously researched, and even now, each country is concentrating on developing new pesticides.

As for pest control methods, there are largely organic synthetic pesticide control methods and recently emerging biological control methods. It has become a threat to health, and pesticides remain in agricultural products, raising serious concerns about food safety. In addition, problems such as ecosystem disturbance and increase in resistant harmful organisms are also being caused.

As the background technology of the present invention, Korean Patent Registration No. 10-0638266 relates to "a concentrated mixture of plant extracts having insecticidal activity", which is related to coriander, red ginseng, side vein, yaguk, camellia, jinrang, sagunja, baekji, yero, etc. By mixing all the extracts, the concentrated mixture of plant extracts was prepared to prevent rice gnats, cabbage moths, spotted mites, tobacco weed moths, thrips, oyster flies, greenhouse whiteflies, green worms, nightshade moths, deep-eating moths, slugs, aphids, rice water weevils, Ihwamyeong moths, A technique for using it as an insecticide for gnat moth is disclosed.

In addition, Korean Patent Publication No. 2005-0115521 relates to "a natural pesticide as a biological pesticide using plant extracts", which is one of mercury extract (neem oil), white root extract, arborvitae extract, white poplar extract, and castor extract. There are reports on various insect control effects using various insecticidal plant extract compositions prepared by combining two or more species.

Korean Patent Registration No. 1996152 relates to 'a sulfur granular wettable composition with excellent water disintegration and dispersion stability and a manufacturing method thereof', containing 55 to 65% by weight of sulfur-bentonite powder having a particle size of 800 to 1,000 mesh and 5 to 8% by weight of a dispersant. A sulfur granular hydration agent composition with improved disintegration in water and dispersion stability, containing 8 to 12% by weight of a dispersing aid, 8 to 12% by weight of a binder, and 12 to 16% by weight of an extender, sulfur having a particle size of 800 to 1,000 mesh -Bentonite powder is formed by mixing bentonite with molten sulfur, forming into pellets, cooling, first dry grinding, adding surfactant, thickener and water, and then second wet grinding, sulfur-bentonite liquid phase with a particle size of 800 to 1,000 mesh It is prepared by lyophilization after forming a hydration liquid, and the dispersant is a maleic acid-di-isobutylene copolymer, a maleic acid-isobutylene copolymer, a naphthalene sulfonic acid formaldehyde condensate, and a carboxylated A mixture of two or more selected from the group consisting of naphthalene sulfonate formaldehyde condensate, the dispersing aid is at least one selected from the group consisting of KCl, sodium sulfonate and urea, and the binder is calcium lignin Sulfonate or sodium lignosulfonate, the bulking agent is diatomaceous earth, the particle size of the composition is 1 to 25 mm, the disintegratability in water is that the composition is dispersed in water within 15 seconds, and the dispersion stability is in water A sulfur granular hydration agent composition characterized in that the dispersed composition has a 325 mesh pass rate of 93% or more has been proposed.

Biological pesticides, such as natural pesticides and natural enemy microorganisms, are being actively studied to replace the above chemical pesticides, but most natural pesticides and natural enemy microorganisms have weak control effects or extremely limited effects, and are expensive. As a result, it is not effective and economically viable, so it has not succeeded as a substitute for chemical pesticides to realize eco-friendly agriculture.

Korean Patent Registration No. 10-0638266 is "Plant Extract Concentrated Mixture with Insecticidal Activity" Korean Patent Publication No. 2005-0115521 "Natural insecticide as a biopesticide using plant extract" 3) Republic of Korea Patent Registration No. 1996152 'Sulfur granular hydration agent composition with excellent water disintegration and dispersion stability and manufacturing method thereof'

The present invention is intended to solve the above problems, and the control composition for crop pests capable of effective water treatment and soil treatment is effective and mass production is possible, and economical water treatment and soil treatment are possible for crop pests, and their Its purpose is to provide a manufacturing method.

The present invention is tea tree extract. It is intended to provide a control composition for crop pests capable of water treatment and soil treatment, characterized in that it is formulated in a certain form by mixing neem extract with sulfur powder, diatomaceous earth powder and an auxiliary agent.

In addition, the water surface treatment and soil treatment characterized in that the tea tree is concentrated and extracted by repeatedly washing the tea tree seeds, pulverizing them with a grinder, mixing and extracting the solvent, mixing 90-96% alcohol and concentrating under reduced pressure. It is intended to provide a control composition for possible crop pests.

In addition, the adjuvant is to provide a control composition for crop pests capable of water treatment and soil treatment, characterized in that any one selected from the group consisting of surfactants and water.

In addition, based on 100 parts by weight of the granules, 9 to 11 parts by weight of tea tree extract, 4 to 6 parts by weight of neem extract, 23 to 35 parts by weight of sulfur, 45 to 60 parts by weight of diatomaceous earth and 4 to 6 parts by weight of auxiliary agents are mixed. It is intended to provide a control composition for crop pests capable of water treatment and soil treatment.

In addition, (a) crushing the tea tree and mixing the extracted extract with sulfur powder and diatomaceous earth powder; (b) mixing neem extract; (c) mixing the adjuvant; (d) to provide a method for producing a control composition for crop pests capable of water treatment and soil treatment, characterized in that it comprises the step of formulating and drying in a certain form using a molding machine.

In addition, the water surface treatment and soil treatment characterized in that the tea tree is concentrated and extracted by repeatedly washing the tea tree seeds, pulverizing them with a grinder, mixing and extracting the solvent, mixing 90-96% alcohol and concentrating under reduced pressure. It is intended to provide a method for producing a control composition for possible crop pests.

In addition, the adjuvant is to provide a method for producing a control composition for crop pests capable of water treatment and soil treatment, characterized in that any one selected from the group consisting of surfactants and water.

In addition, based on 100 parts by weight of the granules, 9 to 11 parts by weight of tea tree extract, 4 to 6 parts by weight of neem extract, 23 to 35 parts by weight of sulfur, 45 to 60 parts by weight of diatomaceous earth and 4 to 6 parts by weight of auxiliary agents are mixed. It is intended to provide a method for producing a control composition for crop pests capable of water treatment and soil treatment.

The control composition for crop pests capable of water treatment and soil treatment of the present invention and its manufacturing method are effective in controlling crop pests capable of water treatment and soil treatment, and are economically very useful because mass production is possible.

The following drawings attached to this specification illustrate a preferred embodiment of the present invention, and together with the detailed description of the present invention serve to further understand the technical idea of the present invention, the present invention is limited to those described in the accompanying drawings. It should not be construed as limiting.
1 is a flow chart schematically showing a method for preparing a control composition for crop pests capable of water treatment and soil treatment of the present invention.
Figure 2 is a diagram showing the manufacturing process of tea tree seed extract in the present invention.

Below, the present invention will be described in detail with reference to the embodiments presented in the accompanying drawings, but the presented embodiments are illustrative for a clear understanding of the present invention, and the present invention is not limited thereto.

Hereinafter, the technical configuration of the present invention according to a preferred embodiment will be described in detail.

The present invention is a surface treatment for insect pest control using two or more main components such as neem extract, plant extracts (mulberry, Yangmyeongju, cinnamon tree, cloves, tea tree extract, etc.), sulfur, deris extract, camphor tree oil, and cinnamon oil as raw materials. It relates to a control composition for crop pests capable of soil treatment and a method for manufacturing the same.

The manufacturing method of the control composition for crop pests capable of water treatment and soil treatment of the present invention is described in detail in order as follows.

1 is a flow chart schematically showing a method for preparing a control composition for crop pests capable of water treatment and soil treatment of the present invention.

First, the tea tree is pulverized and the extracted extract is mixed with sulfur powder and diatomaceous earth powder. (a).

In the present invention, tea tree extract is used as a plant extract.

The tea tree is an evergreen broad-leaved shrub, and its scientific name is Camellia sinensis . Camellia means the genus Camellia. In Korea , Camellia sinensis var. sinensis is cultivated in Jeolla-do, Gyeongsang-do and Jeju-do. Camellia sinensis var. assamica is cultivated in India and Sri Lanka.

It is a subtropical evergreen shrub that grows as a small tree depending on the environment. Trim to a height of about 1 meter for the convenience of harvesting. Branches diverge a lot. First-year branches are white and have fine hairs, and second-year branches are grayish-brown and hairless. Leaves are usually alternate, lanceolate, oblong, with green leaf veins on the surface, gray-green veins protruding from the back, and no hairs on both sides. Flowers bloom in October-November, 3-5cm in diameter, white, fragrant, 1-3 axillary or hanging at the end of branches. The fruits ripen in the fall of the following year, and the seeds are round and hard.

The seeds of the tea tree are also used for cosmetics and food, and the oil squeezed from the seeds is used as fertilizer or livestock feed and as a substitute for soap.

Figure 2 is a diagram showing the manufacturing process of tea tree seed extract in the present invention.

As shown in FIG. 2, the tea tree extract is concentrated and extracted by repeating the process of washing the tea tree seeds, pulverizing them with a grinder, mixing and extracting the solvent, mixing 90-96% alcohol and concentrating under reduced pressure.

After 100 g of tea tree seeds were pulverized with a pulverizer, a 20-fold solvent of the pulverized sample was put into a flask containing the pulverized sample. After that, each sample is placed in a shaker set at an extraction temperature of 50 ° C and extracted over night. The extraction process is finished and each sample is filtered using gauze and filter paper. proceed with

<Experimental Example 1> The sample after the filtration process was concentrated under reduced pressure using alcohol and water, and the results shown in Tables 1 and 2 were shown.

Sample classification Heating Temp.(℃) Cooling Temp.(℃) Amount of concentrated sample (ml) water 50~55℃ 10℃ 200ml 50% alcohol 40~45℃ 10℃ 190ml 75% alcohol 40~45℃ 10℃ 195ml Alcohol 95% 40℃ 10℃ 180ml

The sample after the filtration process was concentrated under reduced pressure to obtain 1/10 vol. When concentrated to such an extent, it is concentrated as shown in Table 1.

Check the final concentration and yield for each sample. sample name tea tree seeds Alcohol 95% extract Alcohol 75% extract Alcohol 50% extract water extract Primary extract (ml) 1,700 1,800 1,750 1,850 Concentrate (ml) 190 195 190 200 ^※ Yield (%) 11.2 10.8 10.9 10.8

※ Yield (%): concentrate/primary extract × 100

As a result of the concentration test, the use of 95% alcohol showed the highest yield with 11.2%.

<Experimental Example 2>

As a result of concentration loss, crude saponin analysis was performed, and the results are shown in Table 3.

※ Analysis method: The Shibata method (1965) was applied to the analysis of saponin, and the Shibata method is an analysis method accepted in scientific journals related to medicinal crops, including Korea J. Medicinal Crop Sci.

After accurately measuring the weight of 2 g of the sample, 100 ml of 80% methanol solution was added and extracted for 4 hours in a water bath at 70 ° C. The extract was concentrated under reduced pressure to remove methanol, and the concentrated extract was dissolved again in 50 ml of distilled water, put into a separatory funnel, and degreased twice with 70 ml of Ethyl Ether. The water layer was extracted three times with 70 ml of saturated butanol (n-Butyl Alcohol), and the butanol layer was concentrated under reduced pressure. Thereafter, the final concentrate was dried at 105 ° C to remove moisture, and then the amount of crude saponin was quantified.

sample name Sample weight (g) flat bottom
Weight of flask (g)
A After final concentration drying
Weight of flask (g)
B saponin
content (%) Alcohol 95% extract 2.0336 190.1327 191.1810 51.5490 Alcohol 75% extract 2.0556 183.1429 183.3905 12.0451 Alcohol 50% extract 2.0480 160.6860 160.9233 11.5869 water extract 2.0600 182.6627 182.7041 2.0097

※ Content (%): (Final The weight of the flask after concentration and drying (B) - the weight of the original flat bottom flask (A) / the weight of the sample × 100

It was confirmed that the active ingredient content of the composition obtained by extraction with 95% alcohol was less than 4 times and as much as 25 times different from other samples.

-Establishment of optimal extraction conditions according to the extraction process and analysis results of main components of tea tree seeds

Solvent: 95% fermented alcohol

Solvent input: 20 times the sample weight

Extraction condition: 12 hours or more at 50℃ (O/N), Shaking incubation

Concentration method and conditions: concentration under reduced pressure (Heating Temp.: 40℃ / Cooling Temp.: 10℃)

In the present invention, tea tree extract can be mixed with a solid carrier to form various formulations, and sulfur and diatomaceous earth are used as the solid carrier used in the present invention.

Sulfur is one of the natural substances used by humans to control pests and diseases from crops. According to ancient European books, around the 9th century BC, Homer described sulfur as a substance that pests shun. As a fungicide, sulfur began to be used in earnest to control grape powdery mildew as wine production decreased by up to 80% due to grape powdery mildew in France in the 1800s. has come to be widely used.

Pure sulfur is a very stable, insoluble, crystalline yellow solid. Sulfur is an organic material that is allowed to be used in the United States, Europe, and Korea. In addition, it is currently allowed as a raw material for eco-friendly organic materials that can be used for the purpose of controlling eco-friendly organic diseases and pests in Korea, and products containing sulfur are sold.

In Korea, studies have been reported that the quality of fruits and vegetables and garlic is improved or the disease control effect is achieved when powdered sulfur is applied directly to the soil.

Thereafter, the neem extract is mixed with the mixture of the tea tree extract, sulfur, and diatomaceous earth (b).

Neem belongs to the Meliaceae family and its scientific name is Azadirachta indica .

As a semi-evergreen tree, it grows a lot in subtropical and tropical regions, and more than 50% of them live in India. White flowers bloom in February-March, and the green fruits that run after the flowers wither become yellow when they ripen in July-August and are a favorite of birds. It is resistant to drying and has high flame resistance. The fruit is edible and is also used as a folk medicine.

Neem bark is cold, bitter, astringent, removes poison, and purifies blood. Neem oil extracted from the fruit has a unique fragrance and is not used for food.

The fruit has a bitter taste and is used as a medicine for hemorrhoids and an insect repellent. The seeds are also bitter in taste and used as an insect repellent and have detoxification. 45% of the oil is extracted from the seeds. It is a bitter oil with a smell similar to garlic and sulfur. It is rich in fatty acids and contains vitamin E and essential amino acids.

The main component, azadirachtin, affects the growth and reproduction of insects and is usefully used as a biological pesticide.

Thereafter, the adjuvant is mixed with the mixture up to step (b) (c).

An adjuvant may be mixed with the mixture and diluted to an appropriate concentration for use.

The adjuvant is used as a diluting solvent, and at least one selected from the group consisting of water and surfactant may be used, for example, water may be used.

Surfactants may include alkyl sulfates, alkyl sulfonates, alkyl arylsulfonates, alkyl aryl ethers and their polyoxy ethylene derivatives, polyethylene glycol ethers, polyhydric alcohol esters and sugar alcohol derivatives.

Then, it is formulated into a certain shape using a molding machine and dried (d).

For mixing, 9 to 11 parts by weight of tea tree extract, 4 to 6 parts by weight of neem extract, 23 to 35 parts by weight of sulfur, 45 to 60 parts by weight of diatomaceous earth, and 4 to 6 parts by weight of auxiliary are mixed based on 100 parts by weight of the granule.

Granular Formulation Mixture Composition Examples division Mixed composition (planned composition) note formulation 1 Tea tree extract 20%+ Neem cake 30%+ Glucose 20%+ Corn starch 10%+ Dolomite 20% formulation 2 Tea tree extract 20%+ Neem cake 20%+ Soybean meal 30%+ Sulfur 15%+ Dolomite 15% formulation 3 tea tree extract 20%+ neem cake 50%+ glucose 10%+ sulfur 10%+ diatomaceous earth 10% formulation 4 tea tree extract 20%+ neem cake 50%+ diatomaceous earth 20%+ supplement 10% formulation 5 tea tree extract 15%+ neem cake 40%+ sulfur 10%+ diatomaceous earth 20%+ supplement 10% formulation 6 10% tea tree extract + 50% neem cake + 15% sulfur + 25% diatomaceous earth formulation 7 10% tea tree extract + 50% neem cake + 15% sulfur + 25% diatomaceous earth formulation 8 20% tea tree extract + 50% neem cake + 10% sulfur + 20% diatomaceous earth formulation 9 30% tea tree extract + 45% neem cake + 10% sulfur + 15% diatomaceous earth formulation 10 10% tea tree extract + 10% neem extract + 30% sulfur + 50% diatomaceous earth formulation 11 20% tea tree extract + 5% neem extract + 25% sulfur + 50% diatomaceous earth formulation 12 30% tea tree extract + 5% neem extract + 20% sulfur + 45% diatomaceous earth formulation 13 10% tea tree extract + 35% neem cake + 30% sulfur + 25% diatomaceous earth formulation 14 Tea tree extract 10%+ Neem extract 5%+ Sulfur 30%+ Diatomaceous earth 50%+ Supplements 5%

In the process of formulation research, “formulation 14”, a granular formulation in the form of pellets (diameter less than 5 mm) that can be sprayed with a fertilizer spreader and manually as a control agent for soil rhizosphere pests through a mixed composition (plan) was selected as the final composition. .

<Experimental Example 3>

An indoor test of efficacy and effectiveness was conducted on the target pest (snail snail).

This is to verify the efficacy of the water surface treatment formulation through the test of the water surface treatment control effect on snails of the mixed composition samples. After confirming that snails were active to some extent, samples for each combination were treated and the parasite rate was investigated before treatment, 1 hour, 3 hours, and 24 hours after treatment through lunar observation.

Control effect according to test agent treatment amount (0.36g/0.36㎡) test material number of treatments
(number of animals) Reproductive rate (%) Prevention (%) 1 hour after treatment 3 hours after treatment 24 hours after processing untreated 40 100 100 100 - formulation 1 40 90 5 0 100 formulation 2 40 60 0 0 100 formulation 3 40 40 0 0 100 formulation 4 40 65 0 0 100 formulation 5 40 87.5 5 0 100 formulation 6 40 95 5 0 100 formulation 7 40 90 5 0 100 test material number of treatments
(number of animals) Reproductive rate (%) Prevention (%) 1 hour after treatment 3 hours after treatment 24 hours after processing untreated 20 (100) (95) (95) - formulation 8 20 95 55 5 95 formulation 9 20 90 80 0 100 formulation 10 20 100 70 0 100 formulation 11 20 85 20 0 100 formulation 12 20 75 5 0 100 test material number of treatments
(number of animals) Reproductive rate (%) Prevention (%) 1 hour after treatment 3 hours after treatment 24 hours after processing untreated 20 (100) (100) (90) - formulation 13 20 15 15 0 100 formulation 14 20 85 70 0 100

※ Control value (%) = (control group parasite rate - test group parasite rate / control group parasite rate) × 100

※ Usage = 1kg/10a (1,000㎡)

<Experimental Example 4>

Efficacy effect field verification test ( in vivo ) experiment on target pests

Lettuce small pointed slug drug control effect test

-Test Methods

Target pest: Small pointed slug ( Deroceras reticulatum )

Test crop (variety): Lettuce (Seonpung No. 2)

processing details

test material Main raw material input amount
(%) drug efficacy test Dilution factor and dosage When and how to process formulation 14 10% tea tree extract + 5% neem extract + 30% sulfur + 50% diatomaceous earth + 5% supplement 2kg/10a Soil treatment before planting
(10/10) untreated - - -

- Effect of drug control on lettuce small pointed slugs (25 days after planting) test material Damaged leaf ratio (%) significant difference
(DMRT) control
(%) Ⅰrepeat Ⅱ Repeat Ⅲ Repeat average formulation 14 5.8 9.0 7.3 7.4 b 57.0 untreated 14.8 20.9 15.8 17.2 a -

C.V.(%) 10.6

Through this test, it was confirmed that the test material, formulation 14, showed statistical significance compared to the untreated group with a control effect of 57.0% on the 25th day after planting against lettuce small snails.

<Experimental Example 5>

Red pepper flower yellow thrips drug control effect test

- Test Methods

Target pest: Yellow flower thrips ( Frankliniella occidentalis )

Test crop (variety): red pepper (green pepper)

processing contents

test material Main raw material input amount
(%) drug efficacy test Dilution factor and dosage When and how to process formulation 14 10% tea tree extract + 5% neem extract + 30% sulfur + 50% diatomaceous earth + 5% supplement 2kg/10a Soil admixture before planting untreated - - -

- Drug control effect against yellow pepper flower thrips (56 days after drug treatment) test material Insect number (mary) significant difference
(DMRT) control
(%) Ⅰrepeat Ⅱ Repeat Ⅲ Repeat average formulation 14 21.0 44.0 38.0 34.3 b 56.2 untreated 88.0 65.0 82.0 78.3 a -

C.V.(%) 28.9

- Effect of drug control on yellow pepper flower thrips (63 days after drug treatment) test material Insect number (mary) significant difference
(DMRT) control
(%) Ⅰrepeat Ⅱ Repeat Ⅲ Repeat average formulation 14 29.0 43.0 39. 37.0 b 52.4 untreated 85.0 70.0 78.0 77.7 a -

C.V.(%) 18.0

Through this test, the test material formulation 14 showed statistical significance compared to the untreated group, with the control effect against pepper yellow thrips being 56.2% after 56 days and 52.4% after 63 days after planting.

The control composition for crop pests capable of water treatment and soil treatment of the present invention as described above and its manufacturing method are effective in controlling crop pests capable of water treatment and soil treatment, and are economically very useful because mass production is possible. there is.

So far, the present invention has been described in detail with reference to the presented embodiments, but those skilled in the art can make various modifications and variations without departing from the technical spirit of the present invention with reference to the presented embodiments. will be. The present invention is not limited by these variations and modifications, but is limited only by the claims appended below.

Claims (8)

In the control composition for crop pests capable of water treatment and soil treatment, which is prepared by mixing tea tree extract and neem extract with sulfur powder, diatomaceous earth powder and an auxiliary agent and formulating in a certain form,
The tea plant is extracted by washing the tea tree seeds, pulverizing them with a grinder, mixing the solvent, extracting, mixing 90-96% alcohol and concentrating under reduced pressure by repeating the process,
The adjuvant is any one selected from the group consisting of a surfactant and water,
Surface treatment characterized by mixing 9 to 11 parts by weight of tea tree extract, 4 to 6 parts by weight of neem extract, 23 to 35 parts by weight of sulfur, 45 to 60 parts by weight of diatomaceous earth and 4 to 6 parts by weight of auxiliary based on 100 parts by weight of the granule And a control composition for crop pests capable of soil treatment. delete delete delete (a) crushing tea trees and mixing the extracted extract with sulfur powder and diatomaceous earth powder;
(b) mixing neem extract;
(c) mixing the adjuvant;
In the method for producing a control composition for crop pests capable of water treatment and soil treatment comprising the steps of formulating and drying in a certain form using a molding machine,
The tea plant is extracted by washing the tea tree seeds, pulverizing them with a grinder, mixing the solvent, extracting, mixing 90-96% alcohol and concentrating under reduced pressure by repeating the process,
The adjuvant is any one selected from the group consisting of a surfactant and water,
Surface treatment characterized by mixing 9 to 11 parts by weight of tea tree extract, 4 to 6 parts by weight of neem extract, 23 to 35 parts by weight of sulfur, 45 to 60 parts by weight of diatomaceous earth and 4 to 6 parts by weight of auxiliary based on 100 parts by weight of the granule And a method for producing a control composition for crop pests capable of soil treatment.
delete delete delete

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