JPS61274265A - Automatic analyzer - Google Patents

Abstract

PURPOSE:To enable a blood analysis in a short time, by performing a drive control of a reactor holder to make a continuous measurement and analysis of all the contents of a plurality of reactors. CONSTITUTION:With a rotary drive control, a holder B transfers a reactor A1 to the first reagent distribution position beta through a counterclockwise scan movement after the end of a sample distribution at the sample distribution position alpha and further does so clockwise to the position epsilon advanced by one step from the sample distribution position alpha after the end of the distribution work of the first reagent at the same position beta corresponding to the measuring item. With a normal/opposite scan operation, the reactor A1 is transferred intermittently by one step to the second reagent distribution position gamma. An optical measuring device F is made up of a closed type cylinder rotation chamber 10 and a light source chamber 12 containing a light source 11 and rotates reciprocatively at a required speed with a required angle irrespective of the rotary operation of the holder B. The angle of rotation is controlled to ensure a scan operation at an angle theta between the first reagent distribution position betaand the second reagent distribution position gamma.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an automatic analyzer that easily performs biochemical analysis and immunological analysis, and particularly relates to an improvement of a so-called single-multi type automatic analyzer.

[Prior art and its problems]

In recent years, with the aim of improving blood testing in the region,
Various small, low-cost automatic analyzers that can easily perform blood tests even in small hospitals have been proposed, and most of them are of the so-called single type or single multi type.

Single-type automatic analyzers continuously analyze different measurement items in one reaction line, and since the reaction container is cleaned by a separate means, there is less risk of carryover, but the light wavelength corresponding to the measurement item is The problem was that switching between the two was complicated and it took a long time to obtain the analysis results.

In addition, most of the single-multi type automatic analyzers are equipped with a reaction container cleaning device, but since different items are analyzed in the same reaction container, carryover in the reaction container is likely to occur. However, the device still had the problem that the separation chamber in which the cleaning device was installed was large and complicated, making it expensive.

[Purpose of the invention]

The present invention was devised in view of the current situation, and its purpose is to use a single method that allows analysis of different items in a short time, and also eliminates the risk of carryover occurring in the reaction vessel. The aim is to provide a small, low-cost automatic analyzer that combines the advantages of the single-multi method and the single-multi method.

[Structure of the invention]

In order to achieve the above object, the present invention includes an automatic analyzer that holds a plurality of reaction containers at a predetermined sample dispensing position into which a required amount of blood sample in the sample container is dispensed at required intervals. A turret-shaped reaction container holder, a reagent container holder that holds a plurality of reagent bottles containing reagents corresponding to measurement items, and a reagent container holder that aspirates the required amount of reagent corresponding to the measurement item from the reagent container and distributes it to a predetermined amount. a first reagent dispensing device and a second reagent dispensing device for dispensing into a corresponding reaction container at a pouring position, and an optical measurement device for optically measuring a reacted blood sample in the reaction container at a predetermined position; The holder scans and transfers the reaction container into which the blood specimen has been dispensed at the sample dispensing position to the first reagent dispensing position, and dispenses the first reagent corresponding to the measurement item at the first reagent dispensing position. The reaction container is then driven and controlled to be reversed and scanned to a position one pitch ahead of the sample dispensing position.

〔Example〕

Hereinafter, the present invention will be described in detail based on an embodiment shown in the accompanying drawings.

The automatic analyzer X according to this embodiment is a single-multi type automatic analyzer, and roughly includes a turret-shaped sampler S, a turret-shaped reagent supply device R, and the sampler S and reagent supply device R. and a reaction vessel holder B juxtaposed between the two.

The sampler S has a plurality of sample containers 1 containing a required amount of the specimen (serum) to be measured, and each sample container 1 is held at equal intervals in a turret-shaped sample holder 2, and is driven by a drive (not shown). The device intermittently transports it to the dispensing position at predetermined timing.

When a predetermined sample container 1 is transferred to the dispensing position in this way, the sample in the same container 1 is transferred to a plurality of reactions at a pull dispensing position (to be described later) via the sample dispensing device C. The required amount is dispensed into A1 of the containers.

Further, in the reagent supply device R, reagent bottles 5 containing a required amount of a reagent such as a first reagent or a second reagent corresponding to a measurement item are held in a turret-shaped bottle holder 6 in a radial manner when viewed from a plane. The holder 6 is driven and controlled by a drive device (not shown) so that the reagent bottle 5 containing the reagent corresponding to the measurement item is transferred forward and backward to each dispensing position.

That is, the bottle holder 6 dispenses a first reagent corresponding to a measurement item via a first reagent dispensing device D1 into a reaction vessel A3 which has arrived at the first dispensing position by rotationally controlling a reaction vessel holder B (to be described later). While aspirating and dispensing the required amount, the first
The second reagent dispensing device D2, which is disposed opposite to the reagent dispensing device D1, corresponds to the measurement item in the reaction container A4 that has arrived at the second dispensing position γ by controlling the rotation of the reaction container holder B. The dispensing devices DI, Dz and the reaction container holder B are driven and controlled in synchronization with each other to aspirate and dispense a required amount of the second reagent. Further, the reagent suction operation of the second reagent dispensing device D2 is controlled to operate with a time difference from that of the first reagent dispensing device D1. This means that the reagent bottle 5a corresponding to the measurement item is transferred to the first dispensing position β,
When stopped, the reagent in the reagent bottle 5b that is stopped at the second partial pressure position does not match the analysis item of the blood sample in the reaction container A4 that has arrived at the second dispensing position. In this case, this is so as not to interfere with controlling the rotation of the bottle holder 6 so as to transfer the matching second reagent to the same position.

Note that the sample dispensing device C and the first and second reagent dispensing devices D1. D2 is suction pump, pipette, and pipette)
1 sample suction position or each reagent suction position, and an elevating device that guides the pipette up and down. After aspirating the reagent, it rises and rotates to the sample discharge position α or the first or second reagent discharge position β, γ, and then descends again to the sample or first reagent discharge position. The second reagent is discharged into the reaction container A3 or A4, and then further ascended and transferred to the pipette cleaning section, after which each of the above operations is repeated in sequence,
The configurations and functions of these parts and the drive control device are similar to those of known sample dispensing devices and reagent dispensing devices, so detailed explanation thereof will be omitted here.

The reversing container holder B is formed in a ring shape 9, and as described above, the required number of reaction containers A can be attached and removed in bottomed holes 20 opened at equal intervals as shown in FIG. accommodated as possible. There is still no hole 20 in this reaction vessel holder B.
A light guiding hole 21 is formed through the holder side wall in a horizontal direction perpendicular to the long axis direction of the opening 20, and the axis of each of these light guiding holes 21 is aligned with the light source light at the optical measurement position. It is opened to match the axis.

The reaction container holder B configured in this manner is rotationally controlled by a drive device not shown. In other words, the holder B scans and moves the reaction vessel A1, which has completed length/pull dispensing at the sample dispensing position α, in the counterclockwise direction in FIG. Next, the reaction vessel Al in which the dispensing work of the first reagent corresponding to the measurement item has been completed is
The rotational drive is controlled so as to scan and transport the sample in the clockwise direction of the drawing to a position ε which is one step ahead of the sample dispensing position α. By this forward and reverse scanning operation, the reaction container Al is intermittently transferred two steps at a time in the direction of the second reagent dispensing position r.

In addition, E in FIG. 1 indicates a stirring device, which stirs and mixes the blood sample, etc. in the reaction container A2 in the stirring position, and its structure and operation are the same as those of known stirring devices, so a detailed explanation thereof will be provided. is omitted here.

The optical measuring device F consists of a closed cylindrical rotary wheel with zero weight and a light source 1.
1 and a light source chamber L2 having a built-in light source chamber L2. The cylindrical rotation chamber 10 has an outer diameter slightly smaller than the inner diameter of the reaction vessel holder B, is coaxial with the holder B, and rotates at a required angle and at a required speed regardless of the rotational operation of the holder B. Rotates back and forth. This rotation angle is at least the first
The rotation is controlled to perform a scanning operation at an angle θ between the reagent dispensing position β and the second reagent dispensing position γ. Inside the cylindrical rotating chamber 10, which is driven and controlled in this manner, there are reflecting mirrors [4] arranged on the Rowland circle 13, and a mirror [4] disposed at a predetermined wavelength position of the light source light reflected by the reflecting mirror [4]. A plurality of light receiving devices (three in the figure, but the number is not limited thereto).

) and a diffraction grating device 16 comprising a light receiving element body 15.
In the peripheral wall 17 of the same room 10, where the light source light enters, a light source light entrance hole 18 is opened to penetrate the peripheral wall 17, and the axis of the opening 18 is as follows.
It is arranged so as to be coaxial with the axis of the light guiding hole 21 . The light source chamber 12 in which the light source 11 is installed is connected to the chamber along the outer circumferential wall of the holter B by a connecting body (not shown) installed from the cylindrical rotation chamber 10 bypassing the reaction vessel holter B. 10 so as to rotate together.

Therefore, the reaction liquid in each reaction container A held in the reaction container holder B is subjected to optical measurement corresponding to the measurement item by the integral rotation of the light source chamber 12 and the cylindrical rotation chamber 10.

Since this optical measurement is similar to the known optical measurement means using a diffraction grating, a detailed explanation thereof will be omitted here.

In the above embodiment, the light source chamber L2 is configured to rotate along the outer circumferential wall of the reaction vessel holder B, and the cylindrical rotation chamber 10 is configured to rotate along the inner circumferential wall of the holder B. Although described above, the same effect can be obtained even if the arrangement is reversed.

In addition, in this invention, after the measurement of the reaction vessel A is completed,
It goes without saying that the reaction container holder B may be removed from the hole 20 of the reaction container holder B and cleaned with another cleaning device, or that the reaction container holder B may be detachably attached to the entire reaction container holder B.

〔Effect of the invention〕

As explained above, the present invention includes a talent-like reaction container holder that holds reaction containers at intervals of a plurality of locations into which a required amount of blood specimen in a sample container is dispensed at a predetermined sample dispensing position; A reagent container holder that holds multiple reagent bottles containing reagents corresponding to a measurement item, and a reagent container holder that aspirates the required amount of reagent corresponding to a measurement item from the reagent container and dispenses it into the corresponding reaction container at a predetermined reagent dispensing position. a first reagent dispensing device and a second reagent dispensing device for dispensing, and an optical measurement device for optically measuring a reacted blood sample in a reaction container at a predetermined position,
The reaction container holder scans and transfers the reaction container into which the blood specimen has been dispensed at the sample dispensing position to a first reagent dispensing position, and the first reagent corresponding to the measurement item is disposed at the first reagent dispensing position. The automatic analyzer is configured such that the drive is controlled to invert and scan the dispensed reaction container to a position one pitch ahead of the sample dispensing position, and the reaction container after measurement is transferred to the machine. Since it is designed to be reused after being cleaned using an external cleaning device or manually, it is possible to continuously measure and analyze the contents of multiple reaction vessels held in the reaction vessel holder. In addition to being able to perform blood analysis on multiple blood samples in a short time, the entire device is significantly smaller and has a simple configuration, which allows it to be provided at low cost and with the same size. Since different items are not analyzed in different reaction vessels, there is no risk of carryover occurring, and the reliability of measurement accuracy is greatly improved.

[Brief explanation of the drawing]

The drawings show a single-multi type automatic analyzer according to an embodiment of the present invention, in which FIG. 1 is a plan view schematically showing the components of the entire device, and FIG. 2 is a plan view showing the main parts of the device. FIG. 3 is a partially cutaway perspective view. X...Automatic analyzer A...Reaction container B--Reaction container holder C...Sample dispensing device DI...First
Reagent dispensing device D2...Second reagent dispensing device F...Optical measurement device l...Sample container 5a
, 5b... Reagent bottle 6... Bottle holder α
...Sample dispensing position β...First reagent dispensing position γ...Second reagent dispensing position ε...Position one pitch ahead of the sample dispensing position

Claims (1)

[Claims] A turret-shaped reaction container holder that holds a plurality of reaction containers at required intervals into which a predetermined amount of blood specimen in the sample container is dispensed at a predetermined sample dispensing position, and a reagent containing reagents corresponding to measurement items. A reagent container holder that holds a plurality of bottles, a first reagent dispensing device that aspirates a required amount of a reagent corresponding to a measurement item from a reagent container, and dispenses it into a corresponding reaction container at a predetermined reagent dispensing position, and a second reagent dispensing device. The reaction container holder includes a reagent dispensing device and an optical measurement device that optically measures a reacted blood sample in a reaction container at a predetermined position, and the reaction container holder is configured to hold a reaction container into which the blood sample has been dispensed at the sample dispensing position. Scan transfer to the first reagent dispensing position, and invert the reaction container into which the first reagent corresponding to the measurement item has been dispensed at the first reagent dispensing position to a position one pitch ahead of the sample dispensing position. An automatic analyzer characterized in that the drive is controlled so as to scan and transport.