JPH08224240A - Fluorescent diagnosing device - Google Patents

Abstract

PURPOSE: To provide a fluorescent diagnosing device capable of measuring the fluorescent spectrum of a lesion part without generating bleeding in endoscopic fashion and performing the viable inspection of lesion tissue more surely than ever. CONSTITUTION: Cups 7a, 7b for vital inspection are provided at the tip of a lengthy coil sheath 14 insertible to the channel of an endoscope, and they are opened/closed by the operation of a hand side operating part via an operating wire 11. Optical fibers 3 whose tip faces 3A are mounted on the feet of the cups 7a, 7b are inserted to the coil sheath 14, and their rear terminals are connected to an analyzer 4, and when exciting light from a laser 17 is transmitted and the cups 7a, 7b are opened, target tissue is irradiated with the exciting light from the tip faces 3A, and also, fluorescence from the target tissue is fetched, and it is inputted to a spectroscope 18 which separates the fluorescence, and it is judged whether a part is a normal part or the lesion part by the analysis of a spectrum waveform by a computer 5, and a result is displayed on a color monitor 6.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fluorescence diagnostic apparatus for irradiating light endoscopically and observing and biopsying a lesion such as cancer from autofluorescence from a target tissue.

[0002]

2. Description of the Related Art In recent years, autofluorescence from a living body or fluorescence of a drug injected into a living body is detected as a two-dimensional image, and from the fluorescence image, degeneration of living tissue or a disease state such as cancer (for example, type of disease or infiltration). Range) technology for diagnosing
57 and 5042494.

When light is applied to living tissue, fluorescence having a wavelength longer than that of the excitation light is generated. Examples of fluorescent substances in the living body include NADH (nicotinamide adenine nucleotide), FMN (flavin mononucleotide), pyridine nucleotide, and the like, and recently, the correlation between these endogenous substances and diseases is becoming clear. .

As a technique for endoscopically diagnosing a lesion from such fluorescence, Japanese Patent Application No. Hei 5 (1999) filed by the present applicant.
There is -304427. This is to perform imaging by utilizing the fact that autofluorescence generated when tissue is irradiated with 442 nm blue excitation light has a difference in spectrum between normal and abnormal. In this conventional example, the images of the green and red regions of autofluorescence are compared and calculated, and the cancerous part is displayed in pseudo color.

Further, Japanese Unexamined Patent Publication No. 6-38967 discloses a method of removing the tissue by discriminating it from autofluorescence of a lesion such as cancer and fluorescence of HpD. This precedent is
An optical fiber is incorporated into a scalpel or a biopsy needle to diagnose and metastasize cancer during surgery.

[0006]

A method of excising tissue from the fluorescence spectrum during laparotomy has been shown. However, in this prior example, the tissue spectrum was not measured endoscopically and the tissue was not biopsied.
Further, in this prior art example, an optical probe is attached to the tip of a scalpel or a biopsy needle. In laparotomy, the scalpel and biopsy can be lightly pressed to measure the spectrum and then resected, but if a scalpel or biopsy needle is inserted endoscopically, bleeding may occur with the scalpel or needle. is there.

In the fluorescence measurement, bleeding becomes particularly noisy, and it is considered that the fluorescence cannot be detected due to the strong absorption of hemoglobin in blood.

The present invention has been made in view of the above points, and provides a fluorescence diagnostic apparatus which can endoscopically measure a fluorescence spectrum of a lesion portion without bleeding and can more reliably biopsy a lesion tissue. The purpose is to provide.

[0009]

[Means and Actions for Solving Problems] An elongated insertion part that can be inserted into a channel of an endoscope for observing an organ in a body cavity,
An openable and closable cup for collecting tissue provided at the tip of the insertion section, an operation section for opening and closing the cup provided on the proximal side of the insertion section, and an operation section incorporated in the insertion section, A biopsy forceps having an optical fiber for irradiating the transmitted excitation light and capable of taking in fluorescence from the target tissue side in a state where the light emitting end is arranged near the root and the cup is opened; A light source device for injecting excitation light into a fiber; a spectroscope for detecting the fluorescence through the optical fiber and decomposing it into a spectrum; an analyzing device for analyzing the spectrum and diagnosing the property of a target tissue; A display device for displaying analysis information by the device; and irradiating excitation light from the distal end surface of the optical fiber to the target tissue side to be biopsied before bleeding by opening the cup, Since the fluorescence from the target tissue can be captured, it is possible to determine whether the target tissue is a normal site or a lesion site by analyzing the spectral waveform of the captured fluorescence. By performing the biopsy only on the site, the diseased tissue can be more surely biopsied.

[0010]

Embodiments of the present invention will be specifically described below with reference to the drawings. FIG. 1 shows a fluorescence diagnostic apparatus 1 according to the first embodiment of the present invention. The fluorescence diagnostic apparatus 1 according to the first embodiment uses forceps having a biopsy function and an optical diagnostic function,
More specifically, a biopsy forceps having an optical probe is used.

A fluorescence diagnostic apparatus 1 of the first embodiment shown in FIG.
Is a biopsy forceps 2 with an optical diagnostic function as a biopsy forceps having a function of biopsying the site of the target tissue while determining the property of the target tissue by fluorescence, and is incorporated in the biopsy forceps 2 with an optical diagnostic function. The analyzer 4 which supplies the excitation light to the optical fiber 3 and detects the fluorescence from the target tissue, and the data from the analyzer 4 are analyzed to analyze the tissue properties (cancer, polyp, normal, etc.). A computer 5 for discriminating the result, a color monitor 6 as display means for displaying the result, an endoscope (not shown) having a channel through which the biopsy forceps 2 with an optical diagnostic function is inserted, and its peripheral devices (endoscope). And a light source device for supplying illumination light).

The biopsy forceps 2 with an optical diagnostic function comprises a pair of cups 7a for picking up a target tissue at the tip of an elongated forceps insertion portion which is inserted into a channel of an endoscope.
7b is provided to form a biopsy section, and an operation member 10 provided with a finger hook 9 for opening and closing the cups 7a and 7b is provided on the operation section on the rear end side of the forceps insertion section. And an operating slider 8 that is slidable are formed.

In order to open and close the cups 7a and 7b in conjunction with the movement of the operating slider 8, a wire 11 for transmitting a force is inserted into the forceps insertion portion, and the rear end of the wire 11 is the operating slider. 8 is fixed, and the tip of the wire 11 opens and closes the cups 7a and 7b.
It is connected to the cups 7a and 7b, and is connected to the links 12a and 12b forming a link mechanism via a joint portion 13.

The forceps insertion portion is formed of a coil sheath 14 made of, for example, a tightly wound coil.
The wire 11 and the optical fiber 3 are inserted inside, and the coil sheath 14 protects the wire 11 and the optical fiber 3 in a bendable state. The rear end side of the coil sheath 13 is fixed to the distal end side of a sheath fixing member 15 provided with a branch portion that branches obliquely rearward, and the operating member 10 provided with a finger hook 9 on the rear side of the sheath fixing member 15, There is provided an operating slider 8 which is slidable with respect to the operating member 10 and has a rear end of the wire 11 fixed.

Further, the optical fiber 3 is inserted into a hollow hole provided in a branch portion which is branched obliquely rearward of the sheath fixing member 15, and is extended from the branch portion to the rear end side.
It is connected to the analyzer 4 via 6.

The analyzer 4 has a laser (device) 17 as a light source for exciting the fluorescence of the tissue, and a spectroscope 18 for separating the fluorescence from the tissue into a spectrum.
In the state where the connector cable 16 is connected, the end face of the optical fiber 3 and the laser 17 face each other, and a dichroic mirror 19 for separating the laser light and the fluorescence is arranged in the optical path between them, and the laser light emitted from the laser 17 is To Penetrate. The fluorescence transmitted by the optical fiber 3 is reflected by the dichroic mirror 19 and is incident on the opposing spectroscope 18 through the filter 20 that cuts the excitation light. The laser 17 is formed of, for example, a He-Cd laser, and this He-Cd laser generates laser light having a wavelength of 442 nm as excitation light.

In this embodiment, the tip side of the optical fiber 3 incorporated in the biopsy forceps 2 with an optical diagnostic function is attached so that the tip surface 3A of the optical fiber 3 is located at the roots of the cups 7a and 7b. In this state, when the cups 7a and 7b are opened, the tip surface 3A of the optical fiber 3 is exposed. The tissue side to be biopsied is characterized by having a structure capable of irradiating excitation light from the distal end surface 3A and taking in fluorescence from the tissue side (in other words, by biopsy procedure). Fluorescence observation can be performed before bleeding).

Next, the operation will be described. First, the biopsy forceps 2 with an optical diagnostic function is introduced into a body cavity through an endoscope channel (not shown) that is previously inserted into the body cavity. Then, the cups 7a and 7b at the tip of the biopsy forceps 2 with an optical diagnostic function are brought close to the tissue that is considered to be a lesion, and the operation slider 8 is used.
Slide in the direction opposite to the finger hook 9 to move the cup 7a,
Open 7b. Further open, the cup 7a, 7b
The tip end surface 3A of the optical fiber 3 at the root of is contacted with the tissue.

At this time, the excitation light from the laser 17 is incident on the optical fiber 3 to irradiate the tissue. The optical fiber 3 receives the autofluorescence from the tissue, and the dichroic mirror 1
The fluorescence is reflected at 9 and the excitation light is cut by the filter 20, and then enters the spectroscope 18. That is, the dichroic mirror 19 transmits the excitation light and reflects the fluorescence having a longer wavelength.

The spectrum is separated by the spectroscope 18, and the spectrum intensity is sent to the computer 5 side, and the computer 5 calculates the spectrum waveform intensity in the entire wavelength range of fluorescence, and at the same time, the normal site and the abnormal site such as a lesion site. The spectral wavelength intensities at the two wavelengths that show a marked difference are analyzed, and the analysis result as to whether the target tissue is a normal site or an abnormal site such as a lesion is displayed on the color monitor 6. The surgeon can perform a biopsy procedure (with bleeding) if the abnormal site is highly likely to be an abnormal site, so that the diseased tissue can be biopsied more reliably, and the biopsy can be performed from many sites unnecessarily. Since it is not necessary to carry out a test, the patient's pain can be reduced.

According to this embodiment, the following effects can be obtained. Since it can be determined by the actual biopsy procedure whether the target tissue is normal or abnormal before bleeding, it is possible to realize an environment in which the diseased tissue can be biopsied more reliably.

Next, a fluorescence diagnostic apparatus of a second embodiment for performing fluorescence diagnosis using an endoscope without using biopsy forceps will be described with reference to FIG. The apparatus shown in FIGS. 2 to 4 has a function of auxiliary light source means or auxiliary light source. First, the background will be described.

In the conventional example, an excitation light source and a high-sensitivity camera therefor are connected during fluorescence observation, and during normal observation, switching is performed after connecting to a white light source and a camera therefor. Since weak fluorescence was detected in the fluorescence observation, the observation was performed in close proximity. However, when it is inserted into a wide space such as the stomach, the distance between the eyepiece and the tissue becomes large, resulting in a dark image, and orientation is difficult to attach. Also, when bleeding, there was a problem that it became dark due to the absorption of light.

In view of this, the present embodiment realizes a good orientation that enables imaging with reflected light without changing the conditions for fluorescence observation (such as camera sensitivity and laser intensity) even in such a case. In order to achieve this object, a fluorescence diagnostic apparatus provided with an auxiliary light source means has the following configuration.

The fluorescence diagnostic apparatus 21 of the second embodiment shown in FIG.
Is a laser 22 for exciting fluorescence and a laser diode (hereinafter abbreviated as LD) which is an auxiliary light source during fluorescence observation.
And a light source device 24 in which 23 is incorporated.
Light guide 25 for irradiating light from the inside of the body cavity
An endoscope 27 having a built-in image guide 26 for transmitting the fluorescence and reflected light as a two-dimensional image image.
A camera 28 for converting the fluorescent image and the reflected light image obtained from the endoscope 27 into a video signal, a processing device 29 for processing the video signal, and a color monitor 30 as a display device.

The light source device 24 generates a laser 22 for generating excitation light and an L for generating illumination light for imaging with reflected light.
D23, a driver 31 for driving the LD 23, a controller 32 for controlling the driver 31 and the laser 22, lenses 33, 34, 35, 36 for guiding these lights to the light guide 25, light of the laser 22, and LD2.
And a dichroic mirror 37 that forms a combining means for combining the three lights by transmitting and reflecting. The laser 22 is formed of, for example, a He-Cd laser.
The e-Cd laser emits laser light having a wavelength of 442 nm as excitation light.

The endoscope 27 is formed with an elongated insertion portion 41 that can be inserted into the body cavity 38, an operation portion 42 formed at the rear end of the insertion portion 41, and a rear end of the operation portion 42. And a light guide cable 44 extending from the side of the operation unit 42, and the connector 45 at the end of the light guide cable 44 can be detachably connected to the light source device 24. it can.

The light guide 25 is inserted into the insertion portion 41, the rear end side of the light guide 25 is inserted into the light guide cable 44, and the connector 45 is connected to the light source device 24.
The laser light from the laser 22 is supplied to the end face by connecting to the end face, the laser light is transmitted, and the excitation light is transmitted from the end face on the distal end side of the insertion portion 41 to the tissue 47 side facing through the illumination lens 46 that diffuses further. As a laser beam.

An observation window is provided adjacent to the illumination window to which the illumination lens 46 is attached, and an objective lens 48 is attached to the observation window to form an image on the side of the tissue 47 irradiated with laser light. Connect to the image position. The front end face of the image guide 26 is arranged at this image forming position, and this image is transmitted and transmitted to the rear end face on the eyepiece 43 side. An eyepiece lens 49 is arranged in the eyepiece portion 43 so as to face the rear end surface. A detachable camera 28 can arrive at the eyepiece 43.

In the camera 28, the eyepiece lens 4
9, an excitation light cut filter 50 that blocks excitation light that excites fluorescence, a lens 51 that forms an image through the excitation light cut filter 50, and a plurality of filters that pass a specific wavelength of fluorescence. 52a (only one filter 52a arranged in the optical path is shown in FIG. 2), and the rotary filter 5
The motor 5 for rotating the rotary filter 52 in order to switch the plurality of two filters 52a to be arranged in the optical path.
3, an image intensifier 54 that multiplies the fluorescence that has passed through the filter 52a in the optical path, and a CCD 55 that converts the image of the fluorescence that has been multiplied by the image intensifier 54 into an electrical video signal. .

The processing device 29 is composed of a timing roller 56 for driving and controlling the motor 53 and switching the filter 52a, and an image processing device 57 for processing the signal of the CCD 55 and performing image processing for making it easy to see the lesion. It The image processing device 57 also performs signal processing for displaying the image captured by the CCD 55 through the rotary filter 52 in the pseudo color as a color component image during orientation, that is, when the LD 23 is turned on.

The light emission by the LD 23 can be performed by an operator at the time of orientation such as confirmation of the observation position and positioning. More specifically, by operating a switch or the like, the excitation light is independently turned on and off (lighting,
You can turn it off.

The light from the LD 23 is reflected by the rotary filter 5
It matches the wavelength (for example, the green and red wavelengths) of the specific fluorescence extracted by the filter 52a incorporated in the No. 2 filter.
Further, the illuminance of the light is low, and in a typical use state during fluorescence observation by the laser 22, reflected light that is substantially equal to the intensity of specific fluorescence transmitted through the filter 52a incorporated in the rotary filter 52 is filtered during orientation. It is a light source with low illuminance that is incident on the light 52a.

Next, the operation will be described. At the time of fluorescence observation, the laser light of the wavelength which becomes the excitation light is emitted from the laser 22 to the lenses 33,
The light enters the light guide 25 of the endoscope 27 through 4, 35, and is diffused and illuminated by the illuminating lens 46 that diffuses in front of the illuminating lens 46 in the body cavity 38. At this time, fluorescence is generated from the tissue 47 side, and the fluorescence is incident on the camera 28 through the objective lens 48, the image guide 26, and the eyepiece lens 49.

That is, in the camera 28, only the fluorescence is passed by the excitation light cut filter 50, and the specific fluorescence is extracted by the filter (for example, green or red filter) 52a incorporated in the rotary filter 52, and this is imaged. After amplifying the tensioner 54 by several thousand to tens of thousands times,
It is received by the CCD camera 55. At this time, the rotary filter 52
The motor 52 switches the filter 52a incorporated in the rotary filter 52, and the video signals obtained by the respective filters 52a are subjected to processing such as weighting of each signal so that the lesion is emphasized by the image processing device 57. , Are displayed in pseudo color on the color monitor 30, for example.

On the other hand, at the time of orientation, the controller 32 controls the LD 23 that emits light with a wavelength equal to the transmission intensity of the filter 52a incorporated in the rotary filter 52.
And driver 31 to turn on the light, and dichroic mirror 37
It is reflected by and is combined with the light of the laser 22.

Similar to the above, these lights are guided to the endoscope 27, and the reflected light is received by the objective lens 48 and the image guide 26. Since the wavelength of the LD 23 passes through the excitation light cut filter 50 and the rotation filter 52, the image irradiated on the LD 23 is multiplied by the image intensifier 54 as a reflected light image and is received by the CCD 55. Then, since the inside of the body cavity is displayed in pseudo color on the color monitor 30 by the image of the reflected light, the orientation can be easily attached by observing the image.

Therefore, this embodiment has the following effects. During fluorescent observation, when the fluorescent image becomes dark due to widening of the space or bleeding, by illuminating with a low-illumination light source such as LD, you can observe the inside of the body cavity without burning to the image intensifier. You can do it, and it will be easier to orient. An LED may be used instead of the LD 23.

Modifications of the embodiment of FIG. 2 are shown in FIGS. 3 and 4, respectively. The modified examples of FIGS. 3 and 4 irradiate the laser 22 as light in the blue region and the LDs 48 and 52 as light in the green and red regions into the body cavity, and detect the reflected light as RGB video signals. 3 shows the structure of the light source device 24 ', and FIG. 4 shows the structure of the camera 28' because it enables observation close to normal white light.

A light source device 24 'shown in FIG. 3 uses an LD 58 which emits light in a green region in place of the LD 23 in the light source device 24 in FIG. 2, and further includes a red region between the lens 36 and the dichroic mirror 37. A dichroic mirror 59 that reflects light and transmits the other light is disposed, and an LD 60 and a lens 61 that face the dichroic mirror 59 and generate light in a red region are disposed. The LDs 58 and 60 are driven by the driver 31,
Both emit light of low illuminance.

In this modification, since the wavelength of the laser 22 is light in the blue region, the dichroic mirror 37 arranged between the lenses 34 and 35 transmits blue light and reflects light having a longer wavelength. Is.

In this structure, the respective lights of the LDs 58 and 60 are combined by transmitting and reflecting by the lenses 36 and 61 and the dichroic mirror 59, and the light of the laser 22 is further combined by the dichroic mirror 37 through the lenses 33 and 34,
The lens 35 collects the light and allows it to enter the light guide 25.

The controller 32 has a switch 3
2a is connected, and the controller 32 operates the LDs 58 and 60 by turning on the switch 32a. In other words, the switch 32a causes L
It is possible to control operation / non-operation of D58 and D60. Other configurations are similar to those of the light source device 24 of FIG.

On the other hand, in the camera 28 'shown in FIG. 4, a dichroic mirror 64 that reflects the wavelength of the blue region of the laser 22 and transmits a longer wavelength, and a light that has passed through the dichroic mirror 64 are further added. A dichroic mirror 65 that reflects green light and transmits longer wavelengths, a CCD 66 that converts the image in the blue region into a video signal, an image intensifier 67 that multiplies the image in the green region, and a video signal It has a CCD 68 for converting it into a signal, an image intensifier 69 for multiplying the image in the red region, and a CCD 70 for converting it into a video signal.

Further, the lenses 71 to 75 transfer the image from the endoscope 27 to the CCD 66 and the image intensifier 6.
It is projected on 7,69. In addition, the filters 76, 7
7 includes the wavelength of the LD 58 and transmits a specific wavelength in the green region, and 7 includes the wavelength of the LD 60 and transmits a specific wavelength in the red region.

The signals photoelectrically converted by the CCDs 66, 68, 70 are input to the image processing device 57, and image processing is performed so that a lesion can be easily identified during fluorescence observation, and CCDs 66, 68 during orientation, The output signal of 70 is regarded as a color component image signal, signal processing is performed to form a color image, and the color signal is displayed on the color monitor 30.

Next, the operation will be described. The fluorescence observation is almost the same as in FIG. 2, but in the embodiment of FIG. 2, specific wavelengths of green and red are obtained by the rotary filter, whereas in the modified examples of FIGS. 3 and 4, the dichroic mirror 65 and the filter are used. 7
6 and 77 to divide into specific wavelengths, and image intensifiers 67 (for green), 69 (for red) and CC
Received with D68 (for green) and 70 (for red).

Then, for example, the same portions in the image images received by the CCDs 68 and 70 are compared,
Change the color of the part that is likely to be an abnormal part, such as a lesion, and change the color or display color depending on the result that is judged to be the part that is likely to be a lesion. Display in pseudo color, for example. For example, the part that is judged to be normal is displayed in white, and the part that is judged to have a possibility of being a lesion is colored red or green, and the higher the probability, the higher the saturation is displayed. Doing so makes it easier to see the potential lesions.

On the other hand, at the time of orientation, the user turns on the switch 32a and causes the driver 31 to cause the LDs 58 and 60 to emit light according to an instruction from the controller 32.

The light from the LDs 58 and 60 is incident on the light guide 25 together with the light from the laser 22 to irradiate an organ or the like in the body cavity. The reflected light is received by the image guide 26, and the image in the blue region is projected on the CCD 66 by the eyepiece lens 49, the lenses 71 to 75, the dichroic mirrors 64 and 65, and the filters 76 and 77, and the image in the green region is image intensifier. Multiplied by 67, CCD6
8, the image in the red area is multiplied by the image intensifier 69 and projected on the CCD 70. The image obtained by each of these is processed by the image processing circuit 57 as an RGB signal and displayed in color on the color monitor 30.

Since the display in this case has almost the same condition as that of the color display by the image pickup under the normal white illumination, it can be observed with the color tone when observed under the white illumination.

This modified example has the following effects. Blue,
By illuminating and receiving the three primary colors of green and red, it is possible to discriminate colors similar to white light observation, so that orientation is further improved.

Next, a third embodiment of the present invention will be described. This embodiment is a fluorescence diagnostic apparatus capable of easily discriminating a difference between a lesion area, a bleeding area, a shadow, etc. by showing both an autofluorescence image and a reflection light source by excitation light on a monitor at the same time. First, the background will be described.

Conventionally, during fluorescence observation, images of wavelengths in the green and red regions of the autofluorescence spectrum were photographed and processed and displayed between the images. Further, the conventional example of Japanese Patent Publication No. 3-58729 discloses that a fluorescence image is standardized with an excitation image.

During fluorescence observation, lesions, bleeding, and shadows are darker than normal, making it difficult to distinguish them. On the other hand, as a method of correcting various fluctuations of fluorescence, a method of standardizing a fluorescence image with a reflection light source has been proposed. In the reflection light source, a lesion part is bright, but a bleeding part and a shadow are still dark.

When the standardization is performed in this state, the bleeding part and the shadow part are standardized by a dark image, and the part is noisy and the image quality is poor.

Therefore, in this embodiment, for the purpose of providing a fluorescence diagnostic apparatus capable of easily distinguishing a lesion area from a bleeding area and a shadow, in order to achieve this purpose, a fluorescence image and a reflected light image are obtained. By displaying both of them as two images on the same monitor, it is possible to easily distinguish the lesion area from the bleeding area and the shadow. This will be specifically described below with reference to FIG.

The fluorescence diagnostic apparatus 81 shown in FIG.
2, a light source device 82 having a built-in light source, a light guide 25 that guides the excitation light emitted from the light source device 82 and irradiates the target tissue inside the body cavity, and an autofluorescence and reflected light image from the tissue. An endoscope 27 having a built-in image guide 26, image intensifiers 67 and 69 for picking up the autofluorescent image, CCDs 68 and 70, and a CCD 66 for picking up the reflected light image, and its optical system (lens 71).
~ 75, dichroic mirrors 64, 65, filter 7
6, 77), and a built-in camera 83, and an image processing device 5 for processing to generate a video signal corresponding to an autofluorescence image.
7, a CCU 84 for driving the reflected image CCD 66 to generate a video signal corresponding to the reflected image, a superimpose circuit 85 for superimposing the two video signals, and a superimpose circuit 85. The auto-fluorescent image 86 and the reflected light image 87 are arranged side by side on the display screen by the video signal.

Next, the operation of this embodiment will be described. First,
Excitation light is emitted from the light source device 82 through the light guide 25 of the endoscope 27, and autofluorescence and reflected light from the tissue are detected by the camera 83 through the image guide 26. At this time, the reflected light of the excitation light is photographed by the CCD 66, and the image intensifier 6 detects the specific wavelengths of the autofluorescent green and red.
7, 69 and CCD 68, 70. Then, after the reflected light image and the fluorescent image are converted into video signals, they are combined by the superimposing circuit 85 and displayed side by side on the monitor 30.

According to this embodiment, the following effects can be obtained. Since the lesion area, the bleeding area, and the shadow are detected as dark images in the fluorescence image, it is difficult to distinguish them. However,
In the reflected light image, the lesion area has a bright reflection image as in the normal area, whereas the bleeding area and the shadow are dark. Therefore, by displaying both the fluorescent image and the reflected image on the monitor at the same time, it is easy to make a distinction. Further, since it is easy to see the biopsy forceps in reflected light imaging, it is considered that a highly accurate biopsy can be performed.

Although the image guide 26 is formed by using the fiber bundle in the endoscope 27 of FIG. 2, for example, in the case of an endoscope in which an image guide or an optical image transmitting means is formed by a relay optical system. Applicable. In addition,
Different embodiments and the like may be configured by partially combining the above-described embodiments and the like, and these embodiments and the like also belong to the present invention.

[Additional Notes] 2. A fluorescence image of a two-dimensional image connected to the endoscope, which has a light source for generating excitation light for generating tissue fluorescence, an endoscope having an image guide for detecting fluorescence from tissue as a two-dimensional image In a fluorescence diagnostic device comprising a camera having an image pickup unit for picking up a fluorescent image of at least one specific wavelength among the above, the illumination light having a wavelength including a part of the at least one specific wavelength is generated in the light source. A low illuminance light source is incorporated, and a synthesizing unit for synthesizing the excitation light and the illumination light, and the illumination light is turned on and off independently of the excitation light.
A fluorescence diagnostic device comprising a control device capable of performing F.

3. The low-illuminance light source has a wavelength in a region including green and red wavelengths, the excitation light has a wavelength included in a blue wavelength band, and the imaging unit corresponds to each of the blue, green, and red wavelengths. The fluorescence diagnostic apparatus according to appendix 2, which captures an image.

4. The fluorescence diagnostic apparatus according to Appendix 2, wherein the low-illuminance light source is an LED or an LD. 5. 3. The fluorescence diagnostic apparatus according to appendix 2, wherein the synthesizing means is a dichroic mirror that reflects and transmits depending on the wavelength of light.

6. The fluorescence diagnostic apparatus according to appendix 2, wherein the excitation light is light of 442 nm emitted from a He-Cd laser.

7. An endoscope having a light source for generating excitation light for generating tissue fluorescence, an endoscope having an image guide for irradiating the excitation light into a body cavity and detecting fluorescence from a tissue as a two-dimensional image, and the endoscope. And a camera having an image pickup means for picking up at least one fluorescence image of a specific wavelength out of the fluorescence image of the two-dimensional image, the reflected light from the tissue due to the excitation light in the camera. A second image pickup means for picking up the image as a two-dimensional image, and a superimpose means for combining both the fluorescent image and the reflected light image on the same screen, and a display means for displaying the superimposed image. Fluorescence diagnostic device.

[0067]

As described above, according to the present invention, a slender insertion portion that can be inserted into a channel of an endoscope for observing an organ in a body cavity and a tissue provided at the tip of the insertion portion are collected. A cup that can be opened and closed, an operation unit that opens and closes the cup provided on the proximal side of the insertion unit, and is incorporated into the insertion unit, and the light emission end is arranged near the root of the cup. In the opened state, a biopsy forceps having an optical fiber for irradiating the transmitted excitation light and capable of taking in fluorescence from the target tissue side; a light source device for making the excitation light incident on the optical fiber; A spectroscope that detects fluorescence through the optical fiber and decomposes it into a spectrum;
Since the fluorescence diagnostic apparatus is composed of an analyzing device for analyzing the spectrum and diagnosing the property of the target tissue; and a display device for displaying analysis information by the analyzing device; When the excitation light is irradiated from the tip surface of the optical fiber before the actual biopsy, and the fluorescence can be captured to make a fluorescence diagnosis of whether or not there is a lesion, and the result shows that there is a high possibility that it is a lesion. Since the biopsy can be actually performed, the lesion can be more surely biopsied.

[Brief description of drawings]

FIG. 1 is a configuration diagram of a fluorescence diagnostic apparatus according to a first embodiment of the present invention.

FIG. 2 is a configuration diagram of a fluorescence diagnostic apparatus according to a second embodiment of the present invention.

FIG. 3 is a configuration diagram of a light source device in a modification of the second embodiment.

FIG. 4 is a configuration diagram of a camera according to a modification of the second embodiment.

FIG. 5 is a configuration diagram of a fluorescence diagnostic apparatus according to a third embodiment of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Fluorescence diagnostic device 2 ... Biopsy forceps with optical diagnostic function 3 ... Optical fiber 3A ... Tip surface 4 ... Analytical device 5 ... Computer 6 ... Color monitor 7a, 7b ... Cup 8 ... Operation slider 9 ... Finger rest 10 ... Operation member 11 ... Operation wire 12a, 12b ... Link 13 ... Joint part 14 ... Coil sheath 15 ... Sheath fixing member 16 ... Relay cable 17 ... Laser 18 ... Spectrometer 19 ... Dichroic mirror 20 ... Filter 21 ... Fluorescence diagnostic device 23 ... LD 25 ... Light Guide 26 ... Image guide 27 ... Endoscope 28 ... Camera 29 ... Processing device 30 ... Color monitor 57 ... Image processing device

Claims (1)

[Claims] 1. An elongated insertion part that can be inserted into a channel of an endoscope for observing an organ in a body cavity, and an openable and closable cup for collecting tissue provided at the tip of the insertion part,
An operating part for opening and closing the cup provided on the proximal side of the insertion part, and a built-in inside the insertion part, in which the light emission end is arranged near the base of the cup and the cup is opened, the transmitted excitation is A biopsy forceps having an optical fiber that irradiates light and can capture fluorescence from the target tissue side; a light source device that makes excitation light incident on the optical fiber;
A spectroscope that detects the fluorescence through the optical fiber and decomposes it into a spectrum; an analyzer that analyzes the spectrum and diagnoses the properties of the target tissue; and a display that displays analysis information by the analyzer. Fluorescence diagnostic device composed of.