JP2009250906A - Analyzing device and sample liquid analyzing method using it - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an analyzing device capable of directly sampling a sample liquid, which is obtained using a puncture implement or a sample injection implement, in both cases using the puncture element and the sample injection element. <P>SOLUTION: The sample liquid is sampled from a first injection port (14) for the sample liquid obtained using the puncture element while the sample liquid is injected in a second injection port (201) for the sample liquid obtained using the sample injection element. The sample liquid sampled from the first injection port (14) is transferred to a holding chamber (5) through a first capillary cavity (4). The sample liquid injected in the second injection port (201) is transferred to the holding chamber (5) through a second capillary cavity (202). <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an analytical device used in analyzing biological fluids electrically or optically.

  Conventionally, as a method of electrochemically analyzing a biological fluid using an analytical device, as a biosensor for analyzing a specific component in a sample solution, for example, glucose in blood and glucose carried in the sensor Some measure the blood glucose level by measuring the current value obtained by reaction with a reagent such as oxidase.

As an optical analysis method, a method of analysis using an analysis device in which a liquid channel is formed is known.
Analytical devices can control fluids using a rotating device with a horizontal axis, and use centrifugal force to quantify samples, separate cytoplasmic materials, transfer and distribute separated fluids, Since the sample can be sent to a test section that performs mixing and stirring, various biochemical analyzes can be performed.

Conventional sampling methods include those found in Patent Document 1 and Patent Document 2.
Patent Document 1 uses a centrifugal force transfer type biosensor 310 as shown in FIG. 14, and a sample is collected from an injection port 313 into a capillary tube 312 by capillary force, and then subjected to centrifugal force. Then, the sample in the capillary 312 is transferred to the receiving cavity 317 via the first channel 314, reacted with the reagent in the receiving cavity 317, and centrifuged, and then only the solution component is supplied to the second cavity 316. Sampled by the capillary force of the core 318, the reaction state is optically read.

  Patent Document 2 uses a centrifugal transfer biosensor 400 shown in FIG. 15, and a sample is transferred from an inlet port 409 to an outlet port 410 with a capillary force, and each capillary 404a to 404f is filled with a sample solution. Thereafter, the centrifugal force generated by the rotation of the biosensor 400 distributes the sample solution in each capillary tube at the positions of the respective vent holes 406a to 406g, and passes through the connecting microconduit 407a to 407f to the next processing chamber ( (Not shown).

As a means for supplying a sample to the biosensors 310 and 400 serving as these analytical devices, it is common to use a shape that allows the sample to be sucked from the sample inlet by capillary force. By bringing a small amount of blood obtained by puncturing a fingertip or earlobe with a puncture tool into contact with the inlet 313 or the inlet port 409, blood is sucked into the analysis device by capillary force. In addition, since blood is sucked by capillary force until it completely fills the capillary, it is supplied to the analysis device by designing so that the capillary communicating with the sample inlet is filled by sucking a specified amount of sample. Generally, a method of making a certain amount of sample to be processed is taken.
Japanese National Publication No. 4-504758 JP-T-2004-529333

  However, in such a conventional configuration, when injecting from a sample injector such as a syringe, dropper or pipette, the tip of the sample injector is brought into contact with the sample inlet of the analytical device, and the sample is brought into contact with the surface tension. It is necessary to suck the sample by capillary force by spotting a small amount that can be held outside the inlet several times. Alternatively, the sample needs to be aspirated by dropping the sample from a sample injection tool onto a sheet-shaped test piece made of plastic or glass and bringing the sample injection port of the analytical device into contact therewith. The work becomes very complicated. In particular, the injection mode with the sample injection tool is often seen in hospitals, clinical laboratories, and research institutions, and the demand from users is high.

  The present invention solves the above-mentioned problems, and the sample injected from the sample injection tool can be sucked so as not to leak from the injection port, and the sample can be quantified by a rotating operation, together with a method using a conventional puncture tool, An object of the present invention is to provide an analytical device capable of collecting a sample from an injection tool.

  According to a first aspect of the present invention, there is provided an analytical device for collecting a sample liquid by capillary force via a first inlet connected to the first inlet and a first inlet for collecting the sample liquid. A first capillary cavity that is capable of receiving, and a holding chamber for receiving sample liquid in the first capillary cavity that is in communication with the first capillary cavity and that is transferred by centrifugal force generated by rotation about an axis. A second inlet for collecting a sample solution different from the first inlet, and a second inlet connected to the second inlet and the holding chamber And a second capillary cavity capable of collecting a sample solution by capillary force via the.

The analysis device according to claim 2 of the present invention is characterized in that, in claim 1, the first capillary cavity and the second capillary cavity are connected.
According to a third aspect of the present invention, there is provided the analytical device according to the first aspect, wherein the first capillary cavity is provided with a cavity continuously communicating with the atmosphere through a gap that does not generate capillary force on one side surface. Features.

The analysis device according to claim 4 of the present invention is characterized in that, in claim 1, the first injection port is opened at the tip of the convex shape.
The analysis device according to claim 5 of the present invention is characterized in that, in claim 1, the bottom surface of the second inlet is an inclined surface so that the sample liquid can easily flow toward the second capillary cavity. And

The analysis device according to claim 6 of the present invention is characterized in that, in claim 1, a recess is provided in a joint portion between the second inlet and the second capillary cavity.
The analysis device according to claim 7 of the present invention is characterized in that, in claim 1, a filter for separating blood cells and plasma is provided at the second inlet.

The analyzing device according to claim 8 of the present invention is characterized in that, in claim 2, the second capillary cavity has a siphon structure.
The analyzing device according to claim 9 of the present invention is characterized in that, in claim 1, the second inlet is connected to the overflow chamber via the overflow channel.

  According to a tenth aspect of the present invention, there is provided a sample liquid analyzing method, comprising: a first capillary cavity connected to a first inlet for collecting a sample liquid and capable of collecting the sample liquid by capillary force; a first capillary cavity; A holding chamber for receiving the sample liquid in the first capillary cavity that is communicated and transferred by centrifugal force generated by rotation around the axis, and for collecting a sample liquid different from the first inlet In an analytical device having a second inlet, and a second capillary cavity connected to the second inlet and the holding chamber and capable of collecting a sample liquid by capillary force through the second inlet In the sample liquid analysis method, when the sample liquid is directly injected into the analysis device, the sample liquid is injected from the first inlet and supplied to the holding chamber, and the sample liquid is supplied via the sample injector. When entering, it is injected from the second inlet, supplied to the holding chamber through the second capillary cavity, and accessed and read the sample liquid transferred from the holding chamber to the measuring chamber. To do.

  In the sample liquid analysis method according to claim 11 of the present invention, a first capillary cavity connected to a first inlet for collecting a sample liquid and capable of collecting the sample liquid by capillary force, a first capillary cavity, A holding chamber for receiving the sample liquid in the first capillary cavity that is communicated and transferred by centrifugal force generated by rotation around the axis, and for collecting a sample liquid different from the first inlet An analytical device having a second inlet, and a second capillary cavity connected to the second inlet and the holding chamber and capable of collecting a sample solution by capillary force through the second inlet. An analysis apparatus to be mounted, wherein the analysis device is held in a state where the first injection port faces the axial center side and rotates around the axial center; and the first injection port or the first injection device 2 inlets The sample liquid collected and held in at least the first capillary cavity of the first capillary cavity and the second capillary cavity is retained by the centrifugal force generated by the rotation by the rotation driving means. Analyzing means for accessing and reading the sample liquid transferred from the holding chamber to the measurement chamber after being transferred to the chamber.

  According to this configuration, the sample injected from the sample injection tool can be sucked so as not to leak from the second injection port, and the sample can be quantified by a rotating operation. Together with the method using the conventional puncture tool, the sample injection tool It is possible to provide an analytical device capable of collecting a plurality of samples.

Embodiments of an analysis device, an analysis method using the same, and a sample liquid injection method according to the present invention will be described with reference to FIGS.
1 and 2 show an analysis device 3 according to an embodiment of the present invention.

  The analysis device 3 is formed by bonding an upper substrate 1 and a lower substrate 2, and a microchannel structure having a fine uneven shape is formed on one surface of the lower substrate 2, and a sample solution Each function works such as transfer of liquid and holding a predetermined amount of liquid. The upper substrate 1 and the lower substrate 2 are bonded to each other by a well-known bonding method such as ultrasonic bonding or UV bonding. After bonding, the upper substrate 1 and the lower substrate 2 can be opened and closed around the shaft 1a in order to prevent scattering of the sample liquid. A protective cap 41 is attached.

  The first inlet 14 is formed in the upper substrate 1 so as to close the groove-shaped first capillary cavity 4 formed in the lower substrate 2 and the opening in the longitudinal direction of the first capillary cavity 4. It is the opening of the entrance side comprised with the convex-shaped protrusion part 4b (refer FIG. 4).

  The second inlet 201 includes a recess 201b formed in the lower substrate 2 and a hole 201a formed in the upper substrate 1 and communicating with the recess 201b. The opening of the hole 201a in use is covered and closed by a lid 41a formed on the protective cap 41.

  FIG. 4 is a perspective view as seen from the periphery of the first injection port 14 of the analysis device 3. The first injection port 14 is a protruding protrusion protruding from one side surface of the analysis device main body. By making it 4b, it becomes easy to spot blood by a fingertip or the like, and there is an effect of preventing the blood from adhering to the position of the finger other than the first inlet 14 when the spot is spotted.

  FIG. 3 shows a state in which one analysis device 3 is set on the rotor 101 of the analyzer 100, and the rotor 101 is rotated around the axis 11 by the rotation drive means (not shown) on the analyzer side. It is rotationally driven in a predetermined direction. The analysis device 3 is set on the rotor 101 so that the first inlet 14 faces the direction of the axis 11.

  As shown in FIG. 4, a recess 12 is formed around the first inlet 14 on one side of the analysis device 3. When viewed in a state where the recess 12 is set in the rotor 101, only the shaft center 11 side opens, and further, the recess 12 is recessed toward the outer peripheral direction from the shaft center 11.

  The concave portion 12 has a gently curved structure so that the cross-sectional area of the opening on the axial center side of the concave portion is equal to or larger than the cross-sectional area of the opening on the outer peripheral side of the concave portion. By forming the sample liquid, the sample liquid adhering to the periphery of the first inlet 14 is surely transferred to the back of the recess 12 by the centrifugal force accompanying the rotation of the rotor 101 and further transferred to the lowest position of the recess 12. It becomes easy and it can collect without scattering outside the recessed part 12.

  As shown in FIG. 5, in the analyzer 100, the rotor 101 and the shaft 120 are fixed via a rotor holding member 121, and are driven by couplings 105a and 105b attached to the motor 104, the shaft 120, and the motor shaft, respectively. It is connected. The shaft 120 is rotatably supported by ball bearings 109 a and 109 b attached to the bearing holding member 122. The motor 104 rotates in a desired direction in accordance with a drive command from a central processing unit 301 configured by a CPU or the like by a current applied to the motor 104 via a drive control unit 302 configured by a driver IC or the like. It rotates at the rotation speed. A balancer 102 is arranged on the rotor 101 for balancing with the analyzing device 3 during rotation. The rotating part including the rotor 101 and the analysis device 3 is in a space sealed by the upper housing 106a and the lower housing 106b, and the inside of the housing space is heated by the heaters 112a to 112d.

  In the following, a specific method for measuring a sample solution in the analysis apparatus 100 of the present invention will be described with reference to FIG. 6 for the procedure until the analysis device 3 is inserted into the analysis apparatus. Will be described.

  The user injects the sample solution into the analysis device 3 from the first injection port 14 or the second injection port described later before attaching the analysis device 3 to the analysis apparatus 100. Then, the user opens the door 111 provided on the upper housing 106a as shown in FIG. When the door 111 is opened, the clamshell type analysis device holding member 125 rotates about the shaft 125a at the base end, and the tip of the analysis device holding member 125 is opened to the position opened by the door 111. The state shown in FIG.

  By inserting the analysis device 3 into the analysis device holding member 125 and closing the door 111, the analysis device holding member 125 returns to the position indicated by the phantom line, and the analysis device 3 is moved to a predetermined position on the rotor 101. Hold in position.

  Then, the user operates the operation unit 308 provided with an operation button for instructing the start of measurement and starts measurement of the sample liquid component. The central processing unit 301 interprets a command from the user, and the drive control unit 302 drives the motor 104 to rotate or stop the analysis device 3, and finally uses the centrifugal force or capillary force to analyze Is introduced into the measurement chamber 7 of the analytical device 3 (see FIG. 2).

  Prior to introduction into the measurement chamber 7, the plasma is colored by causing an enzyme reaction with a reagent (not shown) composed of an enzyme, a dye, a buffer and the like disposed in the holding chamber 5 of the analytical device 3. . At this time, melting and stirring can be promoted by changing the rotational speed of the motor 104 from, for example, 500 rpm to 1500 rpm and applying acceleration, or repeating clockwise, counterclockwise and forward and reverse rotational motions. The colored reaction solution is transferred to the measurement chamber 7 and irradiated with the light source 108, and the transmitted light is detected by the detector 107. Absorbance is obtained from the ratio of reflected light to incident light, and the concentration of a specific component is calculated by the central processing unit 301 based on a calibration curve held in the memory unit 309 and displayed on the display unit 307.

  In addition, when the sample liquid is a blood sample, it is generally temperature-dependent and affects the measurement time and measurement accuracy, so at least the temperature after starting the reagent reaction is constant (30 ° C. to 37 ° C.). Preferably there is. Therefore, in the analysis apparatus 100, the heater 112 is controlled by the temperature control unit 306 based on the detection result of the temperature data processing unit 305 of the temperature sensor 129 so that the temperature becomes about 37 ° C. at the start of the reaction with the reagent. The air temperature is controlled. Thus, by making the air temperature in the housing constant, it becomes possible to uniformly heat the analyzing device 3 without unevenness.

  The analysis apparatus 100 transfers the liquid in the analysis device 3 by using the centrifugal force generated by the rotation around the axis according to the configuration of the chamber and the flow path in the analysis device 3 according to the application. Or a centrifuge that centrifuges.

  The shape of the analysis device 3 may be a fan shape, a cube shape, or other shapes, and a plurality of these analysis devices 3 may be mounted on the rotor 101 simultaneously.

Next, the microchannel configuration of the analysis device 3 of the first embodiment and the sample liquid transfer process will be described in detail.
FIG. 7 shows a microchannel configuration of the analytical device 3 shown in FIGS. FIG. 8 shows the process of transfer from the sample liquid injection to the measurement chamber when the sample liquid is injected from the first inlet 14. FIG. 9 shows an example of the capillary cavity and the cross-sectional shape of the cavity.

  As shown in FIG. 7, the microchannel configuration of the analysis device 3 holds a first injection port 14 for collecting a sample solution and a predetermined amount of the sample solution injected into the first injection port 14. And a first capillary cavity 4 for siphoning, a second capillary cavity 202 having a siphon structure, a cavity 15 for exhausting air in the first capillary cavity 4, and an analysis reagent (not shown) are held. The holding chamber 5, the measurement chamber 7 for measuring the mixture of the sample solution and the analysis reagent, the flow channel 6 communicating with the holding chamber 5 and the measurement chamber 7, the measurement chamber 7, and the air opening hole 9 are communicated. It is comprised with the flow path 8.

  Here, the depth of the first capillary cavity 4, the flow path 6, and the flow path 8 is 50 μm to 300 μm, but if the sample liquid flows by capillary force, it is not limited to this dimension. Absent. The holding chamber 5, the measurement chamber 7, and the cavities 15 and 16 are formed with a depth of 0.3 mm to 5 mm. This is based on the conditions for measuring the amount of sample solution and absorbance (optical path length, measurement). The wavelength, the reaction concentration of the sample solution, the type of reagent, etc.).

  In order to cause the sample liquid to flow by capillary force, the wall surface of the first capillary cavity 4, the flow path 6, and the flow path 8 is subjected to hydrophilic treatment. As the hydrophilic treatment method, plasma, corona, ozone, fluorine, and the like are used. Examples thereof include a surface treatment method using a gas and a surface treatment with a surfactant or a hydrophilic polymer. Here, hydrophilic means that the contact angle with water is less than 90 °, more preferably less than 40 °.

− Transfer process when sample liquid is introduced into the first inlet 14 −
The transfer process in this case is executed as shown in FIG.
In order to supply the sample solution to the analysis device 3, the sample solution is spotted on the first inlet 14 from one side surface of the analysis device 3 before the analysis device 3 is set in the analysis apparatus 100. . Immediately after the spotting, the sample solution is injected into the first capillary cavity 4 and the second capillary cavity 202 of the siphon structure by a predetermined amount by capillary action as shown in FIG.

  At this time, since the cavity 15 for discharging the air in the first capillary cavity 4 is provided on the side surface of the first capillary cavity 4, the sample liquid is preceded by the side surface portion of the first capillary cavity 4. Instead of the flowing capillary flow, the central portion of the first capillary cavity 4 becomes a capillary flow that flows first, and fills the first capillary cavity 4. Therefore, the sample liquid to be spotted on the first inlet 14 is insufficient during the filling of the first first capillary cavity 4, or the sample liquid is separated from the first inlet 14 during the filling. Even in this case, the sample liquid flows through the central portion of the first capillary cavity 4 in advance by spotting again from the first inlet, and comes into contact with the central portion of the sample liquid held in the capillary cavity. Since the air is filled while being discharged in the direction of the side surface of the cavity 15, no bubbles are generated, and the first capillary cavity 4 can be spotted repeatedly until the predetermined amount of sample liquid can be held. It becomes possible.

  As shown in FIG. 9, the first capillary cavity 4 and the cavity 15 are formed on the side surface on one side of the rectangular first capillary cavity 4 formed in the lower substrate 2, and the first capillary cavity has a thickness dimension of a first capillary cavity. A cavity 15 that is larger than the cross-sectional dimension of 4 is provided. The configuration of the first capillary cavity 4 and the cavity 15 is not limited to this.

  In the configuration shown in FIG. 9, the flow of the sample liquid into the cavity 15 can be suppressed by making the sectional dimension of the cavity 15 in the thickness direction 50 μm or more larger than the sectional dimension of the first capillary cavity 4. The upper limit value of the cross-sectional dimension in the thickness direction of the cavity 15 is not particularly specified, but the upper substrate 1 needs to have rigidity in order to maintain the cross-sectional dimension in the thickness direction of the capillary cavity. It is desirable that the distance from the cavity 15 to the cavity 15 is about 0.5 to 1 mm. In addition, it is necessary to perform a hydrophilic treatment in order to exert a capillary action on the first capillary cavity 4, but it is desirable that the hydrophilic treatment be performed only on the wall surface of the first capillary cavity 4. If the hydrophilic treatment is performed, the sample solution flows into the cavity 15.

  After the first and second capillary cavities 4 and 202 are filled with the sample solution, the analysis device 3 with the protective cap 41 closed as shown in FIG. By rotating the analysis device 3 by the driving means, the sample liquid in the first and second capillary cavities 4 and 202 is held in the holding chamber in which the analysis reagent is supported in advance as shown in FIG. 8B. 5 is transferred by centrifugal force. At this time, the sample liquid in the second capillary cavity 202 is also slightly transferred to the overflow chamber 204.

  The sample liquid that has flowed into the holding chamber 5 is mixed with the analysis reagent carried in the holding chamber 5 by swinging due to the rotation acceleration of the analyzer 100 or diffusion of the liquid while the rotation is stopped. It is also possible to mix by applying an external force that directly vibrates itself.

  Next, when the mixing of the reagent and the sample solution reaches a predetermined level, the sample solution in the holding chamber 5 reaches the entrance of the measurement chamber 7 through the channel 6 by capillary force as shown in FIG. Be transported.

  Next, with the rotation of the analyzer 100, as shown in FIG. 8 (d), the sample liquid in the flow path 6 is transferred into the measurement chamber 7, and measurement means (not shown) attached to the analyzer 100 is installed. Thus, the concentration of the component can be measured by measuring the reaction state between the sample solution and the analytical reagent by measuring absorbance or the like.

− Transfer process when the sample liquid is introduced into the second inlet 201 −
The transfer process in this case is executed as shown in FIG. Further, FIG. 11A used for the following description shows a cross-sectional view taken along line AA in FIG. 7, and FIG. 11B shows a cross-sectional view taken along line BB in FIG.

  Before setting the analysis device 3 in the analysis apparatus 100, in this case, the user uses a sample injection tool such as a syringe, dropper or pipette in the hole 201a of the second injection port 201 which is largely opened. To spot. Immediately after the spotting, as shown in FIG. 10A, the sample solution is injected into the first capillary cavity 4 and the second capillary cavity 202 by a predetermined amount by capillary action, and the excess sample solution is injected into the second injection. It remains at the entrance 201.

  At this time, the second injection port 201 has the depression 205 and the inclined surfaces 206a, 206b, and 206c that face the second capillary cavity 202 as shown in FIGS. The liquid naturally moves toward the second capillary cavity 202, and the sample liquid can be transferred to the second capillary cavity 202 and the first capillary cavity 4 by capillary force.

  After the first and second capillary cavities 4 and 202 are filled with the sample solution, the analysis device 3 with the protective cap 41 closed as shown in FIG. As shown in FIG. 10B, the sample liquid in the first and second capillary cavities 4 and 202 is rotated by the driving means of the analyzer 100 so that the holding chamber 5 in which the analysis reagent is supported in advance. It is transferred by centrifugal force. At this time, excess sample liquid in the second capillary cavity 202 is also transferred to the overflow chamber 204 through the overflow channel 203. Since a relatively large amount of excess sample liquid is discharged in the overflow chamber 204, a water absorbing member such as absorbent cotton or a filter paper filter may be disposed in the overflow chamber 204 in order to prevent liquid leakage at the time of disposal. .

  12 and 13 show an example in which the filter member 207 is sandwiched between the upper substrate 1 and the lower substrate 2 so that the filter member 207 is disposed between the hole 201a and the recess 201b of the second inlet 201. Is shown. When the sample is blood, the filter member 207 is formed of a glass filter or the like that allows only plasma to pass through and does not pass blood cells, so that the blood injected from the second inlet 201 is analyzed using plasma, the first As for the blood spotted from the inlet 4, the analysis using whole blood is used, so that the analysis device 3 having the same form can be used for different applications by simply changing the reagent in the holding chamber 5. .

Regarding the subsequent transfer of the reagent solution, FIG. 10 (c) is the same as the description of FIG. 8 (c), and FIG. 10 (d) is the same as the description of FIG. 8 (d). Omitted.
It should be noted that the quantitative property of the sample liquid transferred to the holding chamber 5 can be further improved by providing an air opening hole communicating with the apex of the second capillary cavity 202.

  The rotation drive means in the claims includes the motor 104, the shaft 120, the coupling 105 for connecting the motor 104 and the shaft 120, the rotor 101, the rotor holding member 121, and the drive control unit 302 that controls the motor 104 in FIG. And a central processing unit 301 that controls the drive control unit 302.

  The analysis means in the claims includes the detector 107, the light source 108, the signal processing unit 304 for converting the transmitted light detected by the detector 107 into an electrical signal, and the light source control unit 303 for controlling the output light of the light source 108. The central processing unit 301 controls the signal processing unit 304 and the light source control unit 303 to calculate the absorbance.

  The present invention can contribute to the improvement of biological fluid analysis work.

FIG. 2 is an external view of an analytical device according to an embodiment of the present invention. Exploded perspective view of the analysis device of the same embodiment External perspective view showing an image in which the analyzing device of the embodiment is mounted on the rotor of the analyzing apparatus The perspective view which looked at the device for analysis of the embodiment from the peripheral part of the 1st inlet Central cross-sectional view of the analyzer with the analysis device of the embodiment mounted Central cross-sectional view of the analyzer during mounting of the analysis device of the same embodiment Plan view showing a microchannel configuration of the analysis device of the same embodiment Process drawing of sample solution injection process to filling of measurement chamber when sample solution is injected from first inlet of analysis device of same embodiment Sectional drawing which shows the capillary cavity and the example of a shape of a cavity in the analytical device of the embodiment Process drawing of sample liquid injection process to filling of measurement chamber from second injection port of analytical device of same embodiment AA sectional view of FIG. 7 and BB sectional view of FIG. The disassembled perspective view at the time of arrange | positioning a filter to the 2nd inlet of the device for analysis of the embodiment Plan view when a filter is arranged at the second inlet of the analytical device of the same embodiment Configuration diagram of a conventional centrifugal transfer biosensor The figure explaining sample liquid distribution of the centrifugal transfer type biosensor of the conventional example

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Upper substrate 2 Lower substrate 3 Analytical device 4 1st capillary cavity 5 Holding chamber 6 Flow path 7 Measurement chamber 8 Flow path 9 Atmospheric opening 11 Axis center 14 First inlet 15 Cavity 41 Protective cap 100 Analyzer 101 Rotor 102 Balancer 104 Motor 105 Coupling 106 Housing 107 Detector 108 Light source 109 Ball bearing 111 Doors 112a to 112d Heater 120 Shaft 121 Rotor holding member 122 Bearing holding member 125 Analytical device holding member 129 Temperature sensor 201 Second inlet 202 Second capillary cavity 203 Overflow channel 204 Overflow chamber 205 Indents 206a, 206b, 206c Inclined surface 207 Filter

Claims (11)

A first inlet for collecting the sample liquid, a first capillary cavity connected to the first inlet and capable of collecting the sample liquid by capillary force via the first inlet; An analytical device comprising: a holding chamber for receiving a sample liquid in the first capillary cavity that is communicated with the capillary cavity of the first capillary cavity and transferred by centrifugal force generated by rotation about an axis;
A second inlet for collecting a sample solution different from the first inlet;
An analytical device having a second inlet and a second capillary cavity connected to the holding chamber and capable of collecting a sample solution by capillary force through the second inlet. The analytical device according to claim 1, wherein the first capillary cavity and the second capillary cavity are connected. The analytical device according to claim 1, wherein the first capillary cavity is provided with a cavity communicating with the atmosphere through a gap that does not generate capillary force continuously on one side surface thereof. The analytical device according to claim 1, wherein the first inlet is opened at a convex tip. The analytical device according to claim 1, wherein the bottom surface of the second inlet has an inclined surface so that the sample liquid can easily flow toward the second capillary cavity. The analytical device according to claim 1, wherein a depression is provided in a joint portion between the second inlet and the second capillary cavity. The analytical device according to claim 1, wherein a filter for separating blood cells and plasma is provided at the second inlet. The analytical device according to claim 2, wherein the second capillary cavity has a siphon structure. The analytical device according to claim 1, wherein the second inlet is connected to the overflow chamber via an overflow channel. A first capillary cavity connected to a first inlet for collecting the sample liquid and capable of collecting the sample liquid by capillary force; and a centrifugal force generated by rotation around the axis in communication with the first capillary cavity. A holding chamber for receiving the sample liquid in the first capillary cavity to be transferred; a second inlet for collecting a sample liquid different from the first inlet; a second inlet; A sample liquid analysis method in an analytical device having a second capillary cavity connected to a holding chamber and capable of collecting a sample liquid by capillary force through a second inlet,
When injecting the sample solution directly into the analytical device, the sample solution is injected from the first inlet and supplied to the holding chamber,
When injecting the sample liquid through the sample injector, the sample liquid is injected from the second inlet and supplied to the holding chamber through the second capillary cavity.
A sample liquid analysis method for accessing and reading a sample liquid transferred from the holding chamber to the measurement chamber. A first capillary cavity connected to a first inlet for collecting the sample liquid and capable of collecting the sample liquid by capillary force; and a centrifugal force generated by rotation around the axis in communication with the first capillary cavity. A holding chamber for receiving the sample liquid in the first capillary cavity to be transferred; a second inlet for collecting a sample liquid different from the first inlet; a second inlet; An analysis apparatus to which an analysis device having a second capillary cavity connected to a holding chamber and having a second capillary cavity capable of collecting a sample liquid by capillary force through a second inlet is mounted,
Rotation drive means for holding the analytical device in a state where the first injection port faces the axial center side and rotating around the axial center;
The sample liquid collected from the first inlet or the second inlet and held in at least the first capillary cavity of the first capillary cavity and the second capillary cavity is rotated by the rotation driving means. And an analysis unit that accesses and reads the sample liquid transferred from the holding chamber to the measurement chamber after being transferred to the holding chamber by the centrifugal force generated along with.

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