CN109363639A - A kind of high speed Noncontact optoacoustic imaging system based on optical path modulation combination sensitivity compensation - Google Patents
A kind of high speed Noncontact optoacoustic imaging system based on optical path modulation combination sensitivity compensation Download PDFInfo
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- CN109363639A CN109363639A CN201811344245.0A CN201811344245A CN109363639A CN 109363639 A CN109363639 A CN 109363639A CN 201811344245 A CN201811344245 A CN 201811344245A CN 109363639 A CN109363639 A CN 109363639A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0093—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy
- A61B5/0095—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy by applying light and detecting acoustic waves, i.e. photoacoustic measurements
Abstract
The invention discloses a kind of high speed Noncontact optoacoustic imaging systems based on optical path modulation combination sensitivity compensation, it is characterised in that: including laser emitting part, reference arm, sample end part, detection end part and data processing division.The present invention triggers the multiple laser pulses of laser sending by issuing multiple pulse signals at equal intervals, sample is excited, the light path of reference arm is modulated simultaneously, make multiple photoacoustic signals being excited at least one except low sensitivity region, and the photoacoustic signal except muting sensitivity is selected to carry out sensitivity compensation, to improve acquisition speed.
Description
Technical field
The present invention relates to sports equipment field more particularly to a kind of high speed based on optical path modulation combination sensitivity compensation are non-
Contact photoacoustic imaging system.
Background technique
Photoacoustic imaging is a kind of imaging mode of Noninvasive, it combines the high contrast of pure optical image technology and pure
The advantages that high-resolution of ultrasonic imaging technique, high-penetration depth.Photoacoustic imaging using biological tissue light absorption otherness into
Row imaging, it can be provided to people about the structure of biological tissue, metabolism, function, molecule comparison, Hemodynamics and base
Because of the relevant information of the various dimensions such as expression, a kind of novel imaging method is provided for field of biomedicine.
Currently, contactless optical acoustic imaging can be divided into according to the difference of the photoacoustic signal detection mode in photoacoustic imaging
With contact photoacoustic imaging.
Contact photoacoustic imaging is the detection method based on acoustic pressure, is mainly carried out using PZT and PVDF PZT (piezoelectric transducer)
The detection of photoacoustic signal.Contact type measurement detector and sample surfaces will be close to.In addition, it is also necessary to consider energy converter and sample room
Acoustic impedance match problem.This is because, due to sample surfaces injustice, causing to press when PZT (piezoelectric transducer) and sample are directly contacted
There can be air layer between electric transducer and sample.The acoustic impedance very different of air and energy converter, ultrasonic wave is in two media
Interface on can generate strong reflection photoacoustic signal is caused to be decayed.Therefore, contact type measurement is needed using couplant.With sample
The signal detection inconvenience for contacting, the two features of couplant coupled signal being needed to make contact optoacoustic, limits its extensive use.
Contactless optical acoustic imaging is the detection method based on optical interference, passes through sample table caused by detection photoacoustic signal
The vibration velocity in face or displacement carry out photoacoustic signal detection.It solves the problems, such as acoustic coupling medium in principle, expands light
The application range of acoustic imaging.Heterodyne method is a kind of method of interferometry, it makes itself and survey by the frequency of change reference signal
It measures and generates a difference on the frequency between signal, after reference signal and measuring signal interference, phase tune is contained in interference signal phase
Measured phase can be obtained by carrying out demodulation to interference signal in item (carrier wave) processed and tested quantifier.But it is because of structure
It is complicated therefore be not widely applied.Homodyne interferometer is fairly simple compared with heterodyne ineterferometer, it is easy to accomplish Noncontact optoacoustic signal
Acquisition.But in order to obtain peak response, zero balancing needs the triggering collection photoacoustic signal when interference signal crosses zero curve.
Interference signal is crossed zero curve and is determined by outside environmental elements, unpredictable, therefore photoacoustic imaging speed is slower.
Entitled " In vivo photoacoustic imaging of blood vessels using a homodyne
The periodical of interferometer with zero-crossing triggering " disclose describe it is a kind of based on zero balancing
Noncontact optoacoustic imaging system, this method are based on Michelson's interferometer, using zero balancing realize the excitation of photoacoustic signal with
Processing.The systematic comparison is simple, and preferably still acquisition speed is excessively slow for system sensitivity and imaging resolution, and practicability is poor.
Entitled " Phase sensitive absolute amplitude detection of surface
The periodical of vibrations using homodyneinterferometry without active stabilization " is public
Open describe it is a kind of based on zero balancing go forward side by side line sensitivity compensation surface vibration detection method.This method passes through measurement vibration letter
The actual measurement amplitude of phase locating for number, computing system transient sensitivity and compensation vibration signal, because being acquired without zero cross fired.
But this method fails to consider the problem that low sensitivity region (close to 0) Calculation of Sensitivity is big by noise jamming, in the region
It is easy to cause overcompensation or undercompensation, leads to image quality decrease.
Summary of the invention
In order to solve the above-mentioned technical problem, the object of the present invention is to provide one kind to be based on optical path modulation combination sensitivity compensation
High speed Noncontact optoacoustic imaging system, guarantee image matter, improve acquisition speed.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
A kind of high speed Noncontact optoacoustic imaging system based on optical path modulation combination sensitivity compensation, including lasing fluorescence portion
Divide, reference arm, sample end part, detect end part and data processing section；
The laser emitting part includes long coherence light source, first annular device and coupler, the light of the long coherence light source
Beam enters coupler coupling through first annular device；
Coupler is connect with reference arm, sample end part respectively, and light beam is divided into two-way light after coupler, enters ginseng all the way
Arm is examined, enters sample end part all the way；
The reference arm includes the first lens, the second lens and the first reflecting mirror；
The sample end part successively includes focusing lens, dichroic mirror, the second reflecting mirror, X-Y scanning galvanometer, condenser lens
And sample stage；
The detection end part includes the second circulator and third circulator, and second circulator and third circulator divide
It is not connected with first annular device and coupler, first second circulator and third circulator are also connected with balance detection
Device, the balanced detector connect DAQ high-speed data acquisition card, the DAQ high-speed data acquisition card connection through high-pass filter
AO module, the signal that a channel of the AO module directly acquires balanced detector output is used for PA sensitivity compensation, another
A acquisition channel is described by the extraction of the signal progress high frequency photoacoustic signal exported using high-pass filter to balanced detector
AO module connecting laser, the laser that the laser issues through third reflecting mirror, focusing lens, condenser lens, dichroic mirror with
Detect light in sample it is photosynthetic be light beam, then focus on sample table through the second reflecting mirror, X-Y scanning galvanometer condenser lens again
Face is to excite photoacoustic signal；
The DAQ high-speed data acquisition card is connect with data processing section；
Wherein the acquisition mode of the DAQ high-speed data acquisition card is to change based on homodyne interferometer and to it
Into the AO control module issues the multiple triggering of pulse signal at equal intervals lasers and issues multiple laser pulses, carries out to sample
Excitation, while being modulated the light path of reference arm, make multiple photoacoustic signals being excited at least one in muting sensitivity area
Except domain, and the photoacoustic signal except muting sensitivity is selected to carry out sensitivity compensation, the specific method is as follows:
S1: in photoacoustic imaging system, sample is irradiated by the short-pulse laser focused, and sample interior local absorption body is to sharp
The absorption of light energy generates ultrasonic wave by thermal-elastic expansion, and ultrasonic signal, which travels to sample surfaces, causes sample surfaces vibration to produce
Raw change in displacement ε (t), DAQ high-speed data acquisition card (13) detect change in displacement, and the signal after balance detection is reduced to
By formula 1 it is found thatFor the sensitivity of system detection vibration, wherein φ (t) is variable quantity, remaining is
Constant with the following method, judges the time t before ε (t) arrival by D (t) waveform to acquire φ (t)p, formula 1 at this time
High frequency signal is 0, therefore can be acquired
Meanwhile formula 1 is available by high-pass filtering
It can be in the hope of surface vibration caused by photoacoustic signal in conjunction with formula 2 and formula 3
The ε (t) acquired is the original signal that photoacoustic signal leads to surface vibration, with sensitivity
It is unrelated；
S2: formula 3 is that high-pass filter eliminates result after low frequency component in S1；
S3: reference arm is modulated: derives the phase i.e. φ (t before knowing photoacoustic signal arrival sample by S1p) certainly
Determine system sensitivity, and the most sensitive spot of whole system i.e. so-called orthogonal points (QPs) at the zero point of interference signal, to guarantee
Three photoacoustic signals have one to leave low sensitivity region, need to be modulated reference arm, and modulation is displaced by piezoelectric ceramics
The movement of platform PZT (29) realizes that reflecting mirror (3) is fixed on piezoelectric ceramics displacement platform PZT (29), and AO module (15) exports mould
The movement of quasi- voltage control piezoelectric ceramics displacement platform PZT (29), the movement speed of piezoelectric ceramics displacement platform PZT (29) are according to sharp
Encourage pulse frequency decision, the reflecting mirror being fixedly connected within the photo-acoustic excitation time three times with piezoelectric ceramics displacement platform PZT (29)
(3) distance for being controlled mobile 330nm (λ/4), since the wavelength of long coherence light source (1) is 1300nm, according to principle of interference light
After path difference change one wavelength corresponding phase 2 π of change, the mobile 330nm of reference arm, reference light back and forth changes reference arm light path
660nm, 660nm correspond to the half of 1300nm, this will make φ (tp) π phase change is introduced, so, three optoacoustic letters being excited
At least one PA oscillation can choose the photoacoustic signal and by algorithm compensation described in S1 to avoid low sensitivity region in number
Sensitivity.In short, excited three times in each scan position while reference arm being cooperated to modulate, can not reduced in conjunction with respective algorithms
The quick scanning of photoacoustic imaging is realized while detectivity.
Further, the AO module triggers laser and DAQ high-speed data acquisition card simultaneously, and then accomplishes more
The Quick Acquisition of road signal.
A kind of imaging of high speed Noncontact optoacoustic imaging system based on optical path modulation combination sensitivity compensation is also disclosed
Method includes the following steps:
A, coherent optical detection photoacoustic signal: long coherence light source issues coherent light, through first annular device and passes through coupler point
For two-way light, light focuses on sample table through focusing lens, dichroic mirror, the second reflecting mirror, X-Y scanning galvanometer and condenser lens all the way
Face, the backtracking after sample surfaces reflect, another way light enter the first lens with reference to arm section, second thoroughly as reference light
Mirror, through reflecting mirror backtracking；The two-way light of backtracking is again split into two-beam after coupler couples, all the way through the second warp
Circulator enters balanced detector, enters balanced detector through third circulator all the way；
The interference signal that two output ends of the second circulator and third circulator export in b, step a is sent to balance
Detector, two channels of high-speed data acquisition DAQ equipment are separately input to after photoelectric conversion, this data collecting card passes through AO mould
Block trigger data acquisition, after the signal that a channel of DAQ high-speed data acquisition card directly acquires balanced detector output is used for
The PA sensitivity compensation in face, and another acquisition channel is by carrying out the signal that balanced detector exports using high-pass filter
The extraction of high frequency photoacoustic signal；For acquiring every time, AO module continuously issues 3 pulse signals at equal intervals, three pulse signals
Effect, which is respectively as follows:, is repeatedly triggered to laser and is excited multiple equally spaced photoacoustic signals and first pulse signal
Three equally spaced photoacoustic signals that trigger data acquisition card excites three pulses after making data collecting card be excited carry out
Photoacoustic signal acquisition；
C excites optoacoustic: in the laser irradiation to sample that laser projects, three times to same point excitation, sample absorbs laser
Photoacoustic signal is generated after energy and causes tissue vibration, thus generates micro-displacement, micro-displacement is passed to liquid table by liquid level
Face is simultaneously amplified, and the detection light in step a detects photoacoustic signal by detecting the micro-displacement of liquid surface, wherein laser
It with guarantee excitation and is visited through the sample in reflecting mirror, focusing lens, condenser lens, dichroic mirror and detection light is photosynthetic for light beam-
Survey is same position, focuses on sample surfaces by reflecting mirror, X-Y scanning galvanometer condenser lens to excite photoacoustic signal；
D, signal acquisition: signal acquisition is controlled by AO module, and AO module is simultaneously to laser and DAQ high-speed data acquisition card
It carries out while triggering, and then accomplish the Quick Acquisition of multiple signals；
The collected data of e, DAQ data collecting card finally transfer to data processing section to carry out data processing.
Compared with prior art, advantageous effects of the invention:
The present invention uses the photoacoustic imaging of non-contact full light, is not necessarily to and sample with the detection of contact photoacoustic imaging ratioing signal
Contact, expands photoacoustic imaging application range；
The present invention is realized fast by the improvement to zero balancing in the case where the simplification for the system that ensure that and sensitivity
Speed scanning, this undoubtedly greatly improves acquisition rate, is obviously improved system availability；
It needs to acquire in zero point before getting rid of, by compensation method, realization is not necessarily non-to be acquired in zero point, is improved and is adopted
Collect speed, while acquiring every time acquire three respectively by being modulated to reference arm all in worst position in order to prevent
Secondary, worst acquisition position is avoided in guarantee at least once.
Detailed description of the invention
The invention will be further described for explanation with reference to the accompanying drawing.
Fig. 1 is that the present invention is based on the structures of the high speed Noncontact optoacoustic imaging system of optical path modulation combination sensitivity compensation to show
It is intended to；
Fig. 2 is typical digitlization interference signal demonstration graph；
Description of symbols: 1, long coherence light source；2, first annular device；3, the first reflecting mirror；4, the second lens；5, first
Lens；6, coupler；7, the second circulator；8, third circulator；9, the third lens；10, the 4th lens；11, the 4th reflecting mirror；
12, balanced detector；13, DAQ high-speed data acquisition card；14, high-pass filter；15, AO module；16, laser；17, third
Reflecting mirror；18, the first focusing lens；19, the first condenser lens；20, the second focusing lens；21, dichroic mirror；22, the second reflection
Mirror；23, X-Y scanning galvanometer；24, sample stage；25, the 5th lens；26, the 6th lens；27, the 5th reflecting mirror；28- second is focused
Lens；29, piezoelectric ceramics displacement platform PZT.
Specific embodiment
As shown in Figure 1, a kind of high speed Noncontact optoacoustic imaging system based on optical path modulation combination sensitivity compensation, including
Laser emitting part, reference arm, sample end part, detection end part and data processing section；
The laser emitting part includes long coherence light source 1, first annular device 2 and coupler 6, the long coherence light source 1
Light beam through first annular device 2 enter coupler 6 couple；
Coupler 6 is connect with reference arm, sample end part respectively, and light beam is divided into two-way light after coupler 6, is entered all the way
Reference arm enters sample end part all the way；
The reference arm includes the first lens 5, the second lens 4 and the first reflecting mirror 3；
The sample end part successively includes the second focusing lens 20, dichroic mirror 21, the second reflecting mirror 22, X-Y scanning vibration
Mirror 23, the second condenser lens 28 and sample stage 24；
The detection end part includes the second circulator 7 and third circulator 8, and second circulator 7 and third are annular
Device 8 is connected with first annular device 2 and coupler respectively, and second circulator 7 and third circulator 8 are also connected with balanced detector
12, the balanced detector 12 connects DAQ high-speed data acquisition card 13, the DAQ high-speed data acquisition through high-pass filter 14
One channel of card 13 directly acquires the signal of the output of balanced detector 12 for PA sensitivity compensation, another acquisition channel is adopted
Collect high-pass filter 14 and the extraction that 12 filtered signals are used for high frequency photoacoustic signal, the AO module are exported to balanced detector
15 connecting lasers 16 and DAQ high-speed data acquisition card 13, the AO module 15 issue the synchronous triggering laser of start pulse signal
Device 16 issues laser pulse and DAQ high-speed data acquisition card 13 acquires.The laser that the laser 16 issues is through third reflecting mirror
17, it is light beam that the first focusing lens 18, the first condenser lens 19, dichroic mirror 21 are photosynthetic with the sample in detection light, then again
Sample surfaces are focused on through the second reflecting mirror 22,23 second condenser lens 28 of X-Y scanning galvanometer to excite photoacoustic signal；Described
7 side of second ring device also sets gradually the 5th lens 25, the 6th lens 26 and the 5th reflecting mirror 27.The third circulator 8 one
Side also sets gradually the third lens 9, the 4th lens 10 and the 4th reflecting mirror 11.
The DAQ high-speed data acquisition card 13 is connect with data processing section；
Wherein the acquisition mode of the DAQ high-speed data acquisition card 13 is to change based on homodyne interferometer and to it
Issue the multiple triggering of pulse signals at equal intervals lasers into, the AO control module 15 and issue multiple laser pulses, to sample into
Row excitation, while being modulated the light path of reference arm, make multiple photoacoustic signals being excited at least one in muting sensitivity
Except region, and the photoacoustic signal except muting sensitivity is selected to carry out sensitivity compensation, the specific method is as follows:
S1: in photoacoustic imaging system, sample is irradiated by the short-pulse laser focused, and sample interior local absorption body is to sharp
The absorption of light energy generates ultrasonic wave by thermal-elastic expansion, and ultrasonic signal, which travels to sample surfaces, causes sample surfaces vibration to produce
Raw change in displacement ε (t), DAQ high-speed data acquisition card 13 detect change in displacement, and the signal after balance detection is reduced to
First item is related with low frequency environments condition variation, and Section 2 is the high-frequency signal of photoacoustic signal.Signal overall performance
To be superimposed high frequency oscillation signal caused by g (t) on tempolabile signal as shown in two a of figure.
By formula 1 it is found thatFor the sensitivity of system detection vibration, wherein φ (t) is variable quantity, remaining is
Constant with the following method, judges the time t before ε (t) arrival by D (t) waveform to acquire φ (t)p, formula 1 at this time
High frequency signal only remains low frequency part for 0, therefore can acquire
Meanwhile formula 1 is available by high-pass filtering
It can be in the hope of surface vibration caused by photoacoustic signal in conjunction with formula 2 and formula 3
The ε (t) acquired is the original signal that photoacoustic signal leads to surface vibration, with sensitivity
It is unrelated；
S2: formula 3 is that high-pass filter 14 eliminates result after low frequency component in S1；
S3: reference arm is modulated: derives the phase i.e. φ (t before knowing photoacoustic signal arrival sample by S1p) certainly
Determine system sensitivity, and the most sensitive spot of whole system i.e. so-called orthogonal points QPs at the zero point of interference signal, to guarantee three
A photoacoustic signal has one to leave low sensitivity region, needs to be modulated reference arm, and modulation passes through piezoelectric ceramics displacement platform
The movement of PZT29 realizes that reflecting mirror 3 is fixed on piezoelectric ceramics displacement platform PZT29, and AO module 15 exports analog voltage control
The movement of piezoelectric ceramics displacement platform PZT29, the movement speed of piezoelectric ceramics displacement platform PZT29 are determined according to driving pulse frequency
, the reflecting mirror 3 being fixedly connected within the photo-acoustic excitation time three times with piezoelectric ceramics displacement platform PZT29 is controlled mobile 330nm
It is corresponding to change a wavelength according to principle of interference optical path difference since the wavelength of long coherence light source 1 is 1300nm for the distance of (λ/4)
After 2 π of phase change, the mobile 330nm of reference arm, reference light makes reference arm light path change 660nm back and forth, and 660nm corresponds to 1300nm
Half, this will make φ (tp) π phase change is introduced, so, at least one PA vibrates in three photoacoustic signals being excited
The photoacoustic signal can be chosen and by algorithm compensation sensitivity described in S1 to avoid low sensitivity region.In short, at each
Scan position excites three times while reference arm being cooperated to modulate, can realization while not reducing detectivity in conjunction with respective algorithms
The quick scanning of photoacoustic imaging.
The AO module 15 triggers laser 16 and DAQ high-speed data acquisition card 13 simultaneously, and then accomplishes multichannel
The synchronous acquisition of signal.
A kind of imaging method of the high speed Noncontact optoacoustic imaging system based on optical path modulation combination sensitivity compensation, including
Following steps:
A, coherent optical detection photoacoustic signal: long coherence light source 1 issues coherent light, through first annular device 2 and passes through coupler 6
It is divided into two-way light, light is poly- through the second focusing lens 20, dichroic mirror 21, the second reflecting mirror 22, X-Y scanning galvanometer 23 and second all the way
Focus lens 28 focus on sample surfaces, and the backtracking after sample surfaces reflect, another way light enters reference arm as reference light
Partial the first lens 5, the second lens 4, through 3 backtracking of reflecting mirror；The two-way light of backtracking through coupler 6 coupling after again
It is secondary to be divided into two bundles light, enter balanced detector 12 through circulator 7 through second all the way, enters balance through third circulator 8 all the way and visit
Survey device 12；
The interference signal that two output ends of the second circulator 7 and third circulator 8 export in b, step a is sent to flat
Weigh detector 12, and two channels of high-speed data acquisition DAQ13 equipment are separately input to after photoelectric conversion, this data collecting card is logical
15 trigger data acquisition of AO module is crossed, a channel of DAQ high-speed data acquisition card 13 directly acquires the output of balanced detector 12
Signal be used for subsequent PA sensitivity compensation, and another acquisition channel acquisition high-pass filter 14 to balanced detector export
12 filtered signals are used for the extraction of high frequency photoacoustic signal；For acquiring every time, AO module (15) continuously issues 3 at equal intervals
Pulse signal, three pulse signal effects, which are respectively as follows:, repeatedly to be triggered laser 16 and excites multiple equally spaced optoacoustics
Signal and first pulse signal trigger data acquisition card three pulses are excited after so that data collecting card is excited three
A equally spaced photoacoustic signal carries out photoacoustic signal acquisition；
C excites optoacoustic: in the laser irradiation to sample that laser 16 projects, three times to same point excitation, sample, which absorbs, to swash
Photoacoustic signal is generated after light energy and causes tissue vibration, thus generates micro-displacement, micro-displacement is passed to liquid by liquid level
Surface is simultaneously amplified, and the detection light in step a detects photoacoustic signal by detecting the micro-displacement of liquid surface, wherein swashing
Light is a branch of through reflecting mirror 17, the first focusing lens 18, the first condenser lens 19, dichroic mirror 21 photosynthetic with the sample in detection light
Light-is focused with guaranteeing that excitation and detection are same position by reflecting mirror 22,23 second condenser lens 28 of X-Y scanning galvanometer
In sample surfaces to excite photoacoustic signal；
D, signal acquisition: signal acquisition is controlled by AO module 15, and AO module 15 is simultaneously to laser 16 and DAQ high-speed data
Capture card 13 is carried out while being triggered, and then accomplishes the Quick Acquisition of multiple signals；
The collected data of e, DAQ data collecting card finally transfer to data processing section to carry out data processing.
During acquisition, 3 generations, three photoacoustic signals are excited altogether to same sampled point, since locating phase difference causes
Sensitivity difference, the photoacoustic spectrum difference Fig. 2 (b) extracted after high-pass filtering.PA1 and PA3 is in muting sensitivity area after compensation
Other than domain, thus it is identical through algorithm compensation amplitude, and PA2 is located at low sensitivity region, sensitivity is close to zero and dry by noise
Disturbing leads to sensitivity compensation result distortion map 2 (c).
Embodiment described above is only that preferred embodiment of the invention is described, and is not carried out to the scope of the present invention
It limits, without departing from the spirit of the design of the present invention, those of ordinary skill in the art make technical solution of the present invention
Various changes and improvements, should all fall into claims of the present invention determine protection scope in.
Claims (3)
1. a kind of high speed Noncontact optoacoustic imaging system based on optical path modulation combination sensitivity compensation, it is characterised in that: including
Laser emitting part, reference arm, sample end part, detection end part and data processing section；
The laser emitting part includes long coherence light source (1), first annular device (2) and coupler (6), the long coherence light source
(1) light beam is coupled through first annular device (2) into coupler (6)；
Coupler (6) is connect with reference arm, sample end part respectively, and light beam is divided into two-way light after coupler (6), is entered all the way
Reference arm enters sample end part all the way；
The reference arm includes the first lens (5), the second lens (4) and the first reflecting mirror (3)；
The sample end part successively includes the second focusing lens (20), dichroic mirror (21), the second reflecting mirror (22), X-Y scanning
Galvanometer (23), the second condenser lens (28) and sample stage (24)；
The detection end part includes the second circulator (7) and third circulator (8), second circulator (7) and third ring
Shape device (8) is connected with first annular device (2) and coupler respectively, and second circulator (7) and third circulator (8) are also connected with
Balanced detector (12), the balanced detector (12) connect DAQ high-speed data acquisition card (13) through high-pass filter (14), institute
A channel for stating DAQ high-speed data acquisition card (13) directly acquires the signal of balanced detector (12) output for PA sensitivity
Compensation, another acquisition channel acquire high-pass filter (14) and are used for high frequency to balanced detector output (12) filtered signal
The extraction of photoacoustic signal, AO module (15) connecting laser (16) and DAQ high-speed data acquisition card (13), the AO module
(15) synchronous triggering laser (16) the sending laser pulse of start pulse signal is issued to adopt with DAQ high-speed data acquisition card (13)
Collection.The laser that the laser (16) issues is through third reflecting mirror (17), the first focusing lens (18), the first condenser lens
(19), it is light beam that dichroic mirror (21) is photosynthetic with the sample in detection light, then again through the second reflecting mirror (22), X-Y scanning vibration
(23) second condenser lens (28) of mirror focuses on sample surfaces to excite photoacoustic signal；
The DAQ high-speed data acquisition card (13) connect with data processing section；
Wherein the acquisition mode of the DAQ high-speed data acquisition card (13) is to change based on homodyne interferometer and to it
Into the AO control module (15) issues the multiple triggering of pulse signal at equal intervals lasers and issues multiple laser pulses, to sample
Excited, while the light path of reference arm be modulated, make multiple photoacoustic signals being excited at least one low sensitive
It spends except region, and the photoacoustic signal except muting sensitivity is selected to carry out sensitivity compensation, the specific method is as follows:
S1: in photoacoustic imaging system, sample is irradiated by the short-pulse laser focused, and sample interior local absorption body is to laser energy
The absorption of amount generates ultrasonic wave by thermal-elastic expansion, and ultrasonic signal, which travels to sample surfaces, causes sample surfaces vibration to generate position
Variation ε (t) is moved, DAQ high-speed data acquisition card (13) detects change in displacement, and the signal after balance detection is reduced to
By formula 1 it is found thatFor the sensitivity of system detection vibration, wherein φ (t) is variable quantity, remaining is normal
Amount with the following method, judges the time t before ε (t) arrival by D (t) waveform to acquire φ (t)p, at this time in formula 1
High-frequency signal is 0, therefore can be acquired
Meanwhile formula 1 is available by high-pass filtering
It can be in the hope of surface vibration caused by photoacoustic signal in conjunction with formula 2 and formula 3The ε acquired
(t) lead to the original signal of surface vibration for photoacoustic signal, it is unrelated with sensitivity；
S2: formula 3 is that high-pass filter (14) eliminate result after low frequency component in S1；
S3: reference arm is modulated: derives the phase i.e. φ (t before knowing photoacoustic signal arrival sample by S1p) decision systems
Sensitivity, and the most sensitive spot of whole system i.e. so-called orthogonal points (QPs) at the zero point of interference signal, to guarantee three light
Acoustical signal has one to leave low sensitivity region, needs to be modulated reference arm, and modulation passes through piezoelectric ceramics displacement platform PZT
(29) movement realizes that reflecting mirror (3) is fixed on piezoelectric ceramics displacement platform PZT (29), AO module (15) output simulation electricity
The movement of voltage-controlled piezoelectric ceramics displacement platform PZT (29) processed, the movement speed of piezoelectric ceramics displacement platform PZT (29) are according to excitation arteries and veins
Rush frequency decision, reflecting mirror (3) quilt being fixedly connected within the photo-acoustic excitation time three times with piezoelectric ceramics displacement platform PZT (29)
The distance for controlling mobile 330nm (λ/4) changes since the wavelength of long coherence light source (1) is 1300nm according to principle of interference optical path difference
Becoming a wavelength corresponding phase and changes 2 π, after reference arm moves 330nm, reference light makes reference arm light path change 660nm back and forth,
660nm corresponds to the half of 1300nm, this will make φ (tp) π phase change is introduced, so, in three photoacoustic signals being excited extremely
Rare PA oscillation can choose the photoacoustic signal and sensitive by algorithm compensation described in S1 to avoid low sensitivity region
Degree.
2. the high speed Noncontact optoacoustic imaging system according to claim 1 based on optical path modulation combination sensitivity compensation,
It is characterized by: the AO module (15) carries out while triggering with DAQ high-speed data acquisition card (13) to laser (16), in turn
Accomplish the synchronous acquisition of multiple signals.
3. system is imaged in the high speed Noncontact optoacoustic according to claim 1 or 2 based on optical path modulation combination sensitivity compensation
The imaging method of system, characterized by the following steps:
A, coherent optical detection photoacoustic signal: long coherence light source (1) issues coherent light, through first annular device (2) and passes through coupler
(6) it is divided into two-way light, light is through the second focusing lens (20), dichroic mirror (21), the second reflecting mirror (22), X-Y scanning galvanometer all the way
(23) and the second condenser lens (28) focuses on sample surfaces, the backtracking after sample surfaces reflect, and another way light is as ginseng
It examines light and enters the first lens (5) with reference to arm section, the second lens (4), through reflecting mirror (3) backtracking；The two of backtracking
Road light is again split into two-beam after coupler (6) couple, and enters balanced detector (12) through circulator (7) through second all the way,
Enter balanced detector (12) through third circulator (8) all the way；
The interference signal that two output ends of the second circulator (7) and third circulator (8) export in b, step a is sent to flat
It weighs detector (12), two channels of high-speed data acquisition DAQ (13) equipment, the acquisition of this data is separately input to after photoelectric conversion
For card by AO module (15) trigger data acquisition, a channel of DAQ high-speed data acquisition card (13) directly acquires balance detection
The signal of device (12) output is used for subsequent PA sensitivity compensation, and another acquisition channel is by utilizing high-pass filter (14)
The extraction of high frequency photoacoustic signal is carried out to the signal of balanced detector output (12)；For acquiring every time, AO module (15) is continuous
3 pulse signals at equal intervals are issued, three pulse signal effects, which are respectively as follows:, repeatedly to be triggered and excited more to laser (16)
A equally spaced photoacoustic signal and first pulse signal trigger data acquisition card so that data collecting card is excited after to three
Three equally spaced photoacoustic signals that pulse is excited carry out photoacoustic signal acquisition；
C excites optoacoustic: in the laser irradiation to sample that laser (16) projects, three times to same point excitation, sample absorbs laser
Photoacoustic signal is generated after energy and causes tissue vibration, thus generates micro-displacement, micro-displacement is passed to liquid table by liquid level
Face is simultaneously amplified, and the detection light in step a detects photoacoustic signal by detecting the micro-displacement of liquid surface, wherein laser
It is photosynthetic through reflecting mirror (17), the first focusing lens (18), the first condenser lens (19), dichroic mirror (21) and the sample in detection light
It is light beam-to guarantee that excitation and detection are same position, is focused by reflecting mirror (22), X-Y scanning galvanometer (23) second
Lens (28) focus on sample surfaces to excite photoacoustic signal；
D, signal acquisition: signal acquisition is controlled by AO module (15), and AO module (15) is simultaneously to laser (16) and DAQ high speed number
It carries out while triggering according to capture card (13), and then accomplish the Quick Acquisition of multiple signals；
The collected data of e, DAQ data collecting card finally transfer to data processing section to carry out data processing.
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