CN109297597B - Phased-difference double-path spectral domain OCT device and method capable of eliminating OCT conjugate mirror image - Google Patents
Phased-difference double-path spectral domain OCT device and method capable of eliminating OCT conjugate mirror image Download PDFInfo
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- CN109297597B CN109297597B CN201811328605.8A CN201811328605A CN109297597B CN 109297597 B CN109297597 B CN 109297597B CN 201811328605 A CN201811328605 A CN 201811328605A CN 109297597 B CN109297597 B CN 109297597B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
- G01J3/26—Generating the spectrum; Monochromators using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
Abstract
The invention relates to a phased difference double-path spectrum domain OCT device capable of eliminating an OCT conjugate mirror image, which comprises a super-radiation light-emitting diode, a collimator, a focusing objective lens, a first beam splitter, a second beam splitter, a third beam splitter, a fourth beam splitter, a first reflector, a sample, a first spectrometer and a second spectrometer; the phase difference of the interference signals is stable, and the imaging depth is doubled without reducing the imaging speed of the system.
Description
Technical Field
The invention relates to a phased difference dual-path spectral domain OCT device and method capable of eliminating OCT conjugate mirror images.
Background
The interference signal generated by the spectral domain optical coherence tomography system is an interference signal of a complex domain and has a real part and an imaginary part, however, the spectrometer of the conventional spectral domain OCT can only collect real part information of the interference signal of the sample. Because of the absence of interference signals, mixing occurs when performing a fast fourier transform, so that two images exist when the system is imaged, namely a real image and a conjugate image. In the traditional method for removing the conjugate mirror image, most of phase shifting methods adopt a data acquisition card to control voltage output so as to drive piezoelectric ceramics to carry out phase shifting. The accuracy and stability of the phase obtained by the method are greatly affected by the performance of piezoelectric ceramics, and signals are required to be acquired at least twice at the same position, so that the imaging speed is influenced, and real-time imaging is not facilitated; the 3×3 optical fiber coupler method is to obtain two phase interference signals by using a 3×3 optical fiber coupler, and can obtain an interference signal with a fixed phase difference, but the 3×3 optical fiber coupler is complicated to manufacture and cannot output two phase difference interference signals with equal interference intensity.
Disclosure of Invention
Therefore, the present invention is directed to a phased differential dual-path spectrum domain OCT device capable of eliminating an OCT conjugate image, which can generate two interference signals with a phase difference of 90 ° at one time, collect the two interference signals synchronously using two spectrometers, and then remove the conjugate image by combining a two-phase de-conjugate image method.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the phased difference double-path spectrum domain OCT device capable of eliminating OCT conjugate mirror image includes super-radiation light-emitting diode, collimator, focusing objective lens, first beam splitter, second beam splitter, third beam splitter, fourth beam splitter, first reflector, sample, first spectrometer and second spectrometer; the light emitted by the super-radiation light-emitting diode is collimated into a beam of parallel light by a collimator; the parallel light is focused by a focusing objective lens, and two beams of light with equal success rate are split by a first beam splitter after being focused, wherein one beam is sample light, and the other beam is reference light; the sample light irradiates the sample, and the reference light irradiates the first reflecting mirror; the back scattered light of the sample is split into an A-port sample light and a B-port sample light with equal success rate through a fourth beam splitter, and the light reflected by the first reflector is split into an A-port reference light and a B-port reference light with equal success rate through a second beam splitter; when the optical path difference of the sample light and the reference light at the port A is within the coherent range of the light source and coincides with the first beam splitter, an interference signal is generated; when the optical path difference of the sample light and the reference light at the port B is within the coherent range of the light source and coincides with the position of the third beam splitter, an interference signal is generated; the interference signal of the A port is emitted into the first spectrometer, and the interference signal of the B port is emitted into the second spectrometer.
Further, the device also comprises a first collecting mirror and a second collecting mirror; the interference signal generated by the port A is introduced into a spectrometer through a first acquisition mirror; the interference signal generated by the port B is introduced into the spectrometer through the second acquisition mirror.
Further, the first spectrometer comprises a first cylindrical lens, a first slit, a second cylindrical lens, a first reflecting mirror, a third cylindrical lens, a first reflecting type line diffraction grating and a first linear array camera; the second spectrometer comprises a sixth cylindrical lens, a second slit, a fifth cylindrical lens, a third reflecting mirror, a fourth cylindrical lens, a second reflection type reticle diffraction grating and a second linear array camera.
Further, the device also comprises an upper computer, wherein the upper computer, the first linear array camera and the second linear array camera are controlled to synchronously collect interference signals with the two phase difference of the sample of 90 degrees.
Further, a control method of a phased differential dual-path spectral domain OCT apparatus capable of eliminating an OCT conjugate image is characterized by comprising the steps of:
step S1, light emitted by a super-radiation light-emitting diode is collimated into a beam of parallel light by a collimator;
s2, focusing the parallel light through a focusing objective lens, and dividing the focused parallel light into two beams with equal success rate through a first beam splitter, wherein one beam is sample light and the other beam is reference light; the sample light irradiates the sample, and the reference light irradiates the first reflecting mirror;
s3, splitting the back scattered light of the sample into an A-port sample light and a B-port sample light with equal success rate through a fourth beam splitter, and splitting the light reflected by the first reflector into an A-port reference light and a B-port reference light with equal success rate through a second beam splitter; when the optical path difference of the sample light and the reference light at the port A is within the coherent range of the light source and coincides with the first beam splitter, an interference signal is generated; when the optical path difference of the sample light and the reference light at the port B is within the coherent range of the light source and coincides with the position of the third beam splitter, an interference signal is generated;
s4, adjusting a third beam splitter and a fourth beam splitter to enable the phase difference between the interference signals of the port A and the interference signals of the port B to be 90 degrees, and injecting the interference signals of the port A into a first spectrometer and the interference signals of the port B into a second spectrometer;
s5, the interference signal enters a spectrometer, is unfolded according to wavelength through a reflection type reticle diffraction grating and is captured by a linear array camera; the interference signal captured by the linear array camera is shown as formula (1):
wherein DC is a direct current signal, and AC is an auto-coherent signal of each layer of the sample arm，Is a function of the light intensity distribution of the light source, "> and />Is the optical path of the sample arm, < >>Is the optical path of the reference arm, ">Wave number;
s6, carrying out signal reconstruction on interference signals with different phases captured by the first linear array camera and the second linear array camera to obtain interference signals of a complex domain;
step S7: and carrying out Fourier transform on the interference signals in the complex domain, and removing the conjugate mirror image to obtain the depth information of the sample.
Further, the step S6 specifically includes:
step S61 simplifying the formula (1) into (4)
（4）
wherein ,for the phase of the interference signal of the respective reflection layer, < >>The phase difference of interference signals of the port A and the port B is obtained;
step S62: the formula expression of the interference signal captured by the linear array camera at the A port is shown as the formula (5):
（5）
the formula of the interference signal captured by the linear array camera at the port B is shown as formula (6):
（6）
after the direct current signals of the reference arm and the sample arm are collected and the direct current signals are deducted, formulas (5) and (6) can be expressed as follows:
（7）
the intensity and phase of the interference signal at each wavelength are calculated by equation (6):
（8）
（9）
step S43: the reconstructed interference signal is expressed as:
（10）。
compared with the prior art, the invention has the following beneficial effects:
according to the invention, two interference signals with the phase difference of 90 degrees can be obtained without a phase shifter, the phase difference of the interference signals is not influenced by the performance of the phase shifter, the anti-interference capability is high, and meanwhile, two spectrometers are adopted to synchronously acquire interference information of the two phases, so that the imaging speed of the system is not reduced.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a graph of the A-port interference spectrum and FFT results in an embodiment of the invention;
FIG. 3 is a graph of the B-port interference spectrum and FFT results in an embodiment of the invention;
FIG. 4 is a graph of the real and imaginary parts of the reconstructed interference signal in an embodiment of the invention;
FIG. 5 is a diagram of the result of the FFT of the reconstructed interference signal in an embodiment of the present invention;
in the figure: the device comprises a 1-super-radiation light emitting diode, a 2-collimator, a 3-focusing objective lens, a 4-first beam splitter, a 5-first collecting lens, a 6-first cylindrical lens, a 7-first slit, an 8-second cylindrical lens, a 9-first reflecting mirror, a 10-third cylindrical lens, a 11-first linear array camera, a 12-first reflecting type line diffraction grating, a 13-second beam splitter, a 14-second reflecting mirror, a 15-upper computer, a 16-second linear array camera, a 17-fourth cylindrical lens, a 18-second reflecting type line diffraction grating, a 19-third reflecting mirror, a 20-fifth cylindrical lens, a 21-second slit, a 22-sixth cylindrical lens, a 23-second collecting lens, a 24-third beam splitter, a 25-sample and a 26-fourth beam splitter.
Detailed Description
The invention will be further described with reference to the accompanying drawings and examples.
Referring to fig. 1, the present invention provides a dual-spectrum domain OCT apparatus capable of eliminating the phasing difference of OCT conjugate mirror, which includes a superradiation led 1, a collimator 2, a focusing objective lens 3, a first beam splitter 4, a second beam splitter 13, a third beam splitter 24, a fourth beam splitter 26, a first reflector 9, a sample 25, a first spectrometer, and a second spectrometer; the light emitted by the super-radiation light-emitting diode 1 is collimated into a beam of parallel light by the collimator 2; the parallel light is focused by a focusing objective lens 3, and is split into two beams with equal success rate by a first beam splitter 4 after being focused, wherein one beam is sample light, and the other beam is reference light; the sample light is directed to the sample 25 and the reference light is directed to the first mirror 9; the back scattered light of the sample 25 is split into an A-port sample light and a B-port sample light with equal success rate through a fourth beam splitter 26, and the light reflected by the first reflector 9 is split into an A-port reference light and a B-port reference light with equal success rate through a second beam splitter 13; when the optical path difference of the sample light and the reference light at the port A is within the coherent range of the light source and coincides with the first beam splitter 4, an interference signal is generated; generating an interference signal when the optical path difference of the sample light and the reference light at the port B is within the coherent range of the light source and coincides with the position of the third beam splitter 24; the interference signal of the A port is emitted into the first spectrometer, and the interference signal of the B port is emitted into the second spectrometer.
In an embodiment of the invention, the device further comprises a first collection mirror 5 and a second collection mirror 23; the interference signal generated by the port A is introduced into a spectrometer through a first acquisition 5 mirror; the interference signal generated by the port B is introduced into the spectrometer through a second collection mirror 23.
In an embodiment of the present invention, the first spectrometer includes a first cylindrical lens 6, a first slit 7, a second cylindrical lens 8, a first reflecting mirror 9, a third cylindrical lens 10, a first reflective reticle diffraction grating 12, and a first line camera 11; the second spectrometer comprises a sixth cylindrical lens 22, a second slit 21, a fifth cylindrical lens 20, a third reflecting mirror 19, a fourth cylindrical lens 17, a second reflective reticle diffraction grating 18 and a second linear array camera 16.
In an embodiment of the present invention, the apparatus further includes a host computer 15, where the host computer 15, the first line camera 11 and the second line camera 16 are controlled to synchronously collect interference signals with two phase differences of the samples being 90 °.
In an embodiment of the present invention, a control method of a phased-difference dual-path spectral domain OCT apparatus capable of eliminating an OCT conjugate image, includes the steps of:
step S1, light emitted by a super-radiation light-emitting diode 1 is collimated into a beam of parallel light by a collimator 2;
s2, focusing the parallel light through a focusing objective lens 3, and dividing the focused parallel light into two beams with equal success rate through a first beam splitter 4, wherein one beam is sample light and the other beam is reference light; the sample light is directed to the sample 25 and the reference light is directed to the first mirror 9;
step S3, the back scattered light of the sample 25 is split into the A-port sample light and the B-port sample light with equal success rate through the fourth beam splitter 26, and the light reflected by the first reflector 9 is split into the A-port reference light and the B-port reference light with equal success rate through the second beam splitter; when the optical path difference of the sample light and the reference light at the port A is within the coherent range of the light source and coincides with the first beam splitter 4, an interference signal is generated; generating an interference signal when the optical path difference of the sample light and the reference light at the port B is within the coherent range of the light source and coincides with the position of the third beam splitter 24;
step S4, adjusting the third beam splitter 24 and the fourth beam splitter 26 to enable the phase difference between the A-port interference signal and the B-port interference signal to be 90 degrees, and injecting the A-port interference signal into the first spectrometer and the B-port interference signal into the second spectrometer;
s5, the interference signal enters a spectrometer, is unfolded according to wavelength through a reflection type reticle diffraction grating and is captured by a linear array camera; the interference signal captured by the linear array camera is shown as formula (1):
wherein DC is a direct current signal, AC is an auto-coherent signal of each layer of the sample arm,is a function of the light intensity distribution of the light source, "> and />Is the optical path of the sample arm, < >>Is the optical path of the reference arm, ">Wave number;
s6, carrying out signal reconstruction on interference signals with different phases captured by the first linear array camera and the second linear array camera to obtain interference signals of a complex domain;
step S7: and carrying out Fourier transform on the interference signals in the complex domain, and removing the conjugate mirror image to obtain the depth information of the sample.
In an embodiment of the present invention, the step S6 specifically includes:
step S61 simplifying the formula (1) into (4)
（4）
wherein ,for the phase of the interference signal of the respective reflection layer, < >>The phase difference of interference signals of the port A and the port B is obtained;
step S62: the formula expression of the interference signal captured by the linear array camera at the A port is shown as the formula (5):
（5）
the formula of the interference signal captured by the linear array camera at the port B is shown as formula (6):
（6）
after the direct current signals of the reference arm and the sample arm are collected and the direct current signals are deducted, formulas (5) and (6) can be expressed as follows:
（7）
the intensity and phase of the interference signal at each wavelength are calculated by equation (6):
（8）
（9）
step S43: the reconstructed interference signal is expressed as:
（10）。
the foregoing description is only of the preferred embodiments of the invention, and all changes and modifications that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (3)
1. The phased difference double-path spectrum domain OCT device capable of eliminating OCT conjugate mirror image is characterized by comprising the following components: the device comprises a super-radiation light-emitting diode, a collimator, a focusing objective lens, a first beam splitter, a second beam splitter, a third beam splitter, a fourth beam splitter, a first reflector, a sample, a first spectrometer and a second spectrometer; the light emitted by the super-radiation light-emitting diode is collimated into a beam of parallel light by a collimator; the parallel light is focused by a focusing objective lens, and two beams of light with equal success rate are split by a first beam splitter after being focused, wherein one beam is sample light, and the other beam is reference light; the sample light irradiates the sample, and the reference light irradiates the first reflecting mirror; the back scattered light of the sample is split into an A-port sample light and a B-port sample light with equal success rate through a fourth beam splitter, and the light reflected by the first reflector is split into an A-port reference light and a B-port reference light with equal success rate through a second beam splitter; when the optical path difference of the sample light and the reference light at the port A is within the coherent range of the light source and coincides with the first beam splitter, an interference signal is generated; when the optical path difference of the sample light and the reference light at the port B is within the coherent range of the light source and coincides with the position of the third beam splitter, an interference signal is generated; the interference signal of the A port is injected into a first spectrometer, and the interference signal of the B port is injected into a second spectrometer;
the first spectrometer comprises a first cylindrical lens, a first slit, a second cylindrical lens, a first reflecting mirror, a third cylindrical lens, a first reflecting type reticle diffraction grating and a first linear array camera; the second spectrometer comprises a sixth cylindrical lens, a second slit, a fifth cylindrical lens, a third reflecting mirror, a fourth cylindrical lens, a second reflection type reticle diffraction grating and a second linear array camera;
the device also comprises an upper computer, wherein the upper computer, the first linear array camera and the second linear array camera are used for controlling the two linear array cameras to synchronously collect interference signals with the two phase differences of the samples of 90 degrees;
wherein the control method of the OCT device comprises the following steps:
step S1, light emitted by a super-radiation light-emitting diode is collimated into a beam of parallel light by a collimator;
s2, focusing the parallel light through a focusing objective lens, and dividing the focused parallel light into two beams with equal success rate through a first beam splitter, wherein one beam is sample light and the other beam is reference light; the sample light irradiates the sample, and the reference light irradiates the first reflecting mirror;
step S3: the back scattered light of the sample is split into an A-port sample light and a B-port sample light with equal success rate through a fourth beam splitter, and the light reflected by the first reflector is split into an A-port reference light and a B-port reference light with equal success rate through a second beam splitter; when the optical path difference of the sample light and the reference light at the port A is within the coherent range of the light source and coincides with the first beam splitter, an interference signal is generated; when the optical path difference of the sample light and the reference light at the port B is within the coherent range of the light source and coincides with the position of the third beam splitter, an interference signal is generated;
step S4: the third beam splitter and the fourth beam splitter are adjusted to enable the phase difference between the interference signals of the port A and the interference signals of the port B to be 90 degrees, the interference signals of the port A are injected into the first spectrometer, and the interference signals of the port B are injected into the second spectrometer;
step S5: the interference signal enters a spectrometer, is unfolded according to the wavelength through a reflection type line diffraction grating and is captured by a linear array camera; the interference signal captured by the linear array camera is shown as formula (1):
I(k)＝DC+AC+∑ n A nr (k)exp[-j2k(z n -z r )] (1)
DC ＝I rr (k)+∑ n I nn (k)(2)
AC＝∑ n≠m A nm (k)exp[-j2k(z n -z m )] (3)
wherein DC is a direct current signal, AC is an auto-coherent signal of each layer of the sample arm, A nr Is the light intensity distribution function of the light source, z n and zm Is the optical path, z, of the sample arm r Is the optical path of the reference arm, k is the wave number;
step S6: carrying out signal reconstruction on interference signals with different phases captured by a first linear array camera and a second linear array camera to obtain interference signals of a complex domain;
step S7: and carrying out Fourier transform on the interference signals in the complex domain, and removing the conjugate mirror image to obtain the depth information of the sample.
2. The phased differential dual path spectral domain OCT apparatus according to claim 1, wherein the OCT conjugate mirror is eliminated, wherein: the device also comprises a first collecting mirror and a second collecting mirror; the interference signal generated by the port A is introduced into a spectrometer through a first acquisition mirror; the interference signal generated by the port B is introduced into the spectrometer through the second acquisition mirror.
3. The phased differential dual path spectral domain OCT apparatus according to claim 1, wherein the OCT conjugate mirror is eliminated, wherein: the step S6 specifically includes:
step S61: simplifying formula (1) into formula (4)
wherein ,phase-combining interference signals for each reflecting layerBit (s)/(s)>The phase difference of interference signals of the port A and the port B is obtained;
step S62: the formula expression of the interference signal captured by the linear array camera at the A port is shown as the formula (5):
the formula of the interference signal captured by the linear array camera at the port B is shown as formula (6):
after the direct current signals of the reference arm and the sample arm are collected and the direct current signals are deducted, formulas (5) and (6) can be expressed as follows:
the intensity and phase of the interference signal at each wavelength are calculated by equation (6):
A nr (k)＝sqrt(I 1 2 +I 2 2 ) (8)
step S43: the reconstructed interference signal is expressed as:
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6002480A (en) * | 1997-06-02 | 1999-12-14 | Izatt; Joseph A. | Depth-resolved spectroscopic optical coherence tomography |
| CN108742511A (en) * | 2018-07-09 | 2018-11-06 | 中国科学院苏州生物医学工程技术研究所 | Spectral coverage OCT and the confocal synchronous scanning system of line |
| CN208937183U (en) * | 2018-11-09 | 2019-06-04 | 福州大学 | A kind of OCT conjugation mirror image of eliminating determines difference two-way spectral coverage OCT device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6002480A (en) * | 1997-06-02 | 1999-12-14 | Izatt; Joseph A. | Depth-resolved spectroscopic optical coherence tomography |
| CN108742511A (en) * | 2018-07-09 | 2018-11-06 | 中国科学院苏州生物医学工程技术研究所 | Spectral coverage OCT and the confocal synchronous scanning system of line |
| CN208937183U (en) * | 2018-11-09 | 2019-06-04 | 福州大学 | A kind of OCT conjugation mirror image of eliminating determines difference two-way spectral coverage OCT device |
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