CN107389420A - A kind of cell enrichment separation method - Google Patents
A kind of cell enrichment separation method Download PDFInfo
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- CN107389420A CN107389420A CN201710659341.3A CN201710659341A CN107389420A CN 107389420 A CN107389420 A CN 107389420A CN 201710659341 A CN201710659341 A CN 201710659341A CN 107389420 A CN107389420 A CN 107389420A
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Abstract
The invention provides a kind of cell enrichment separation method, including：1), first kind magnetic bead and biological specimen are combined, biological specimen includes first kind cell and the second class cell, and first kind magnetic bead is combined with first order antibody, and first kind cell is combined with first kind magnetic bead；2), capture magnetic head is placed in in the biological specimen that first kind magnetic bead is combined, the absorption of first kind cell is on capture magnet arrangement；3), first kind cell is discharged from capture magnetic head assembly, by the first kind cell clearance in biological specimen；4), the second class magnetic bead is added in the biological specimen for having removed first kind cell, the second class cell is combined with the second class magnetic bead；5), capture magnetic head is placed in in the biological specimen that the second class magnetic bead is combined, the absorption of the second class cell is on capture magnet arrangement；6), the second class cell is discharged from capture magnetic head assembly.Detection sensitivity and purity of the present invention are high, and the analysis of the several genes mark available for unicellular or a small amount of cell.
Description
Technical field
The invention belongs to technical field of cell separation, more particularly to a kind of cell enrichment separation method.
Background technology
Circulating tumor cell (CTC) is research tumour diffusion mechanism and tumour early detection, prevention and clinical improvement curative effect
Important symbol thing because its negligible amounts for being present in blood, the requirement to detection technique is very high, and on market today
Technology can not all meet requirement of the in the market as conventional detection project in terms of detection sensitivity and reasonable price.In the market compared with
Ripe detection technique is with positive magnesphere or the exclusive use of negative magnesphere mostly, and it is in detection sensitivity
Be restricted with terms of the subsequent gene analysis of cell, for example, the detection sensitivity of single positive magnesphere by
The target cell purity of limitation and capture is not high, and single negative magnesphere is similarly subjected to non-in terms of the purity of target cell
Often big limitation.
The content of the invention
For the drawbacks described above in background technology, it is a primary object of the present invention to provide a kind of cell enrichment separation side
Method, positive magnesphere and negative magnesphere are combined, improve target cell detection sensitivity, purity and detection
Stability.
In order to achieve the above object, the present invention adopts the following technical scheme that：A kind of cell enrichment separation method, methods described
Including：
1), first kind magnetic bead and biological specimen are combined, the biological specimen includes first kind cell and the second class
Cell, the first kind magnetic bead are combined with first order antibody, by the first order antibody by the first kind cell and first
Class magnetic bead is combined；
2), capture magnetic head is placed in in the biological specimen that the first kind magnetic bead is combined, the first kind cell is inhaled
It is attached on capture magnet arrangement；
3), first kind cell is discharged from capture magnetic head assembly, removes the first kind cell in the biological specimen；
4), the second class magnetic bead is combined with having removed the biological specimen of first kind cell, the second class magnetic bead combines
There is second order antibody, be combined the second class cell with the second class magnetic bead by the second order antibody；
5), capture magnetic head is placed in in the biological specimen that the second class magnetic bead is combined, the second class cell is inhaled
It is attached on capture magnet arrangement；
6), the second class cell is discharged from capture magnetic head assembly；
When the first kind magnetic bead is negative magnetic bead, the second class magnetic bead is positive magnetic bead, and the first kind cell is
Non-target cell, the second class cell are target cell.
As it is further preferably, in the step 3), after first kind cell is discharged from capture magnetic head, caught described
Magnet is obtained to be placed in again with the biological specimen that the first kind magnetic bead is combined, adsorbing the first kind cell again.This operation
Can repeatedly, until reaching the purpose of completely or nearly fully erased first kind cell.
As it is further preferably, in the step 6), after the second class cell is discharged from capture magnetic head, caught described
Magnet is obtained to be placed in again with the second class cell that second magnetic bead is combined, adsorbing the second class cell again.This operation
Also it can be repeated several times, the second class target cell captured to greatest extent until obtaining.
As it is further preferably, the step 3) or 6) in, the capture head surface is provided with magnetosheath, described first
Or second class cell absorption capture magnetic head assembly magnetosheath on.
As it is further preferably, will remaining cell and positive enrichment are collected into after step 5) capture target cell point
It is not collected on container or slide, makees cellular identification, counting or gene recombination analysis.
As it is further preferably, the tumour target cell is used for the more of cell count and unicellular or a small amount of cell
Gene marker is analyzed.
As further preferably, the biological specimen is blood sample or biological fluid, and the non-target cell is including white
Cell and red blood cell, the target cell include circulating tumor target cell.
As further preferably, the blood sample includes former blood sample, the blood sample or close of erythrocyte splitting
Spend gradient cell separating treatment and remove the blood sample after red blood cell.
As it is further preferably, in addition to：When the first kind magnetic bead is positive magnetic bead, second magnetic bead is feminine gender
Magnetic bead, the first order antibody are that target cell identifies antibody, and the secondary antibody is that non-target cell identifies antibody.
As further preferably, the first order antibody is selected from CD45, CD14 and erythrocyte antibody (EA), second class
Antibody is selected from EpCAM, Muc1 and keratin antibody.
The beneficial effects of the invention are as follows：Negative magnesphere and positive magnesphere are combined by the present invention, can be right
Biological specimen first carries out carrying out negative richness again after positive enrichment with magnetic bead or positive enrichment with magnetic bead after negative enrichment with magnetic bead
Collection；Such as：Negative magnetic bead and biological specimen are first combined by the present invention, make the first order antibody and biological specimen on negative magnetic bead
In non-target cell be combined, recycle capture magnetic head absorption non-target cell, by the non-target cell and tumor target in biological specimen
Cell is sufficiently separated；Positive magnetic bead is added in the biological specimen containing the tumour target cell again, after culture, positive magnetic bead
It will be combined with tumour target cell, and now carry out positive enrichment with magnetic bead, and purer positive tumor target cell will be obtained.Therefore,
The present invention can remove non-target cell, such as haemocyte (leucocyte and red blood cell), then divide from a large amount of cells in biological specimen
From tumour cell therein is extracted, its background cells amount greatly reduces, and the purity of the tumour cell finally obtained improves first mate.
Make follow-up cell and genetic analysis using such a product, be beneficial to obtain optimal analysis result.Thus the inventive method
Improve target cell detection sensitivity, purity and the stability of detection.For another example：The present invention can be first by positive magnetic bead and biological sample
Originally it is combined, is combined the tumour target cell in antibody and biological specimen on positive magnetic bead, recycles capture magnetic head absorption
Tumour target cell, the non-target cell in biological specimen is separated with tumour target cell；Negative magnetic bead is added to again described non-
In target cell, negative magnetic bead will be combined with non-target cell, now be carried out negative enrichment with magnetic bead, be obtained non-target cell.Above-mentioned sun
Property the target cell that obtains of enrichment collect counting and carry out cell and genetic analysis, feminine gender enrichment and positive enrichment are remaining thin
Born of the same parents can make cell and the genetic analysis of slide etc., can so play the effect of detection without selection analysis of whole blood cells.
Brief description of the drawings
Fig. 1 a-1b are the cell enrichment separation method of positive enrichment with magnetic bead after the first negative enrichment with magnetic bead of the embodiment of the present invention 1
Schematic flow sheet.
Fig. 1 c are the analysis schematic diagram of the multiple mark of tumour target cell.
Fig. 2 is the cell enrichment separation method flow of negative enrichment with magnetic bead after the first positive enrichment with magnetic bead of the embodiment of the present invention 2
Schematic diagram.
Fig. 3 a-3d are repeatedly the situation schematic diagram that magnetic material is removed in negative enrichment.
Fig. 4 a-4b are the situation schematic diagram of negative enrichment individual cells.
Fig. 5 a-5b are removing situation schematic diagram of the negative concentration method to the target cell of non-magnetic material.
Embodiment
The present invention overcomes existing circulating tumor cell detection technique and examined by providing a kind of cell enrichment separation method
Survey sensitivity and not high in terms of the purity of target cell the defects of.
In order to solve the above problems, the main thought of the embodiment of the present invention is：
Cell enrichment separation method of the embodiment of the present invention, methods described include：
1), first kind magnetic bead and biological specimen are combined, the biological specimen includes first kind cell and the second class
Cell, the first kind magnetic bead are combined with first order antibody, by the first order antibody by the first kind cell and first
Class magnetic bead is combined；
2), capture magnetic head is placed in in the biological specimen that the first kind magnetic bead is combined, the first kind cell is inhaled
It is attached on capture magnet arrangement；
3), first kind cell is discharged from capture magnetic head assembly, removes the first kind cell in the biological specimen；
4), the second class magnetic bead is combined with having removed the biological specimen of first kind cell, the second class magnetic bead combines
There is second order antibody, be combined the second class cell with the second class magnetic bead by the second order antibody；
5), capture magnetic head is placed in in the biological specimen that the second class magnetic bead is combined, the second class cell is inhaled
It is attached on capture magnet arrangement；
6), the second class cell is discharged from capture magnetic head assembly；
When the first kind magnetic bead is negative magnetic bead, the second class magnetic bead is positive magnetic bead, and the first kind cell is
Non-target cell, the second class cell are target cell.
Generally, blood sample after negative magnetic bead combines processing, all will by most red blood cell and leucocyte
With reference to being removed, the embodiment of the present invention can be used repeatable self-reacting device and repeatedly be adsorbed to operate above-mentioned capture magnetic head, can
To reach the degree of the red blood cell and leucocyte fully and completely removed in blood sample.At this moment, positive magnetic bead is added to feminine gender
In sample after enrichment with magnetic bead, positive magnetic bead is attached on magnetic head, or part is attached on cell and then is re-incorporated on magnetic head,
By dead slow speed scanning motion of the magnetic head in sample solution, the target cell in sample solution is adsorbed onto on magnetic head, and turned
Move on in specific collection vessel, the polygenes analysis of markers with unicellular or a small amount of cell is counted for target cell.This hair
Bright embodiment has many advantages, first, being operated more than, whole blood cells are made with the detection and analysis of target cell, the spirit of detection
Sensitivity is very high；On the other hand, the cell for genetic analysis, due to being the non-specific cell number after negative enrichment processing
Amount is considerably less even without being enriched with the positive target cell of extraction sample again therefrom, its purity is very high.Feminine gender enrichment
With cell analysis of the remaining cell after positive be enriched with slide is made, have to prevent and lose what positive enrichment mark was omitted
Target cell.The cell that positive enrichment is extracted makees to count and polygenes detection and analysis, and purity is high, and analysis result is relatively reliable.It is comprehensive
The result of negative and positive enrichment is closed, target cell and analysis gene information is counted, can reach complete equivalent to whole blood cell analysis
Whole effect.Meanwhile target cell purity significantly improves, analysis to the multiple mark such as the gene in downstream provides more excellent
More property.Sample after feminine gender processing, is handled by positive enrichment with magnetic bead, in remaining cell, it is also possible to which existing can not be positive
Property the target cell arrested of magnetic bead, at this moment, by these, to measure few cell, all centrifugation on slide, carries out cell and gene
Horizontal analysis, can obtain more comprehensive target cell (such as：Blood circulation tumour cell etc.) information.Through feminine gender enrichment or sun
Property enrichment after can some remaining free magnetic beads, filtration method can be used to remove the free magnetic bead.
Present invention method, available for the enrichment and separation of cell in sample, also available for other biological molecule into
Point (such as：Nucleic acid and protein) separation and extraction.
In order to which the objects, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiments, to the present invention
It is further elaborated.Specific data involved by specific example described herein are only to explain the present invention, not
For limiting the present invention.
Embodiment 1
As shown in Fig. 1 a-1b, the cell enrichment separation method of the embodiment of the present invention 1, comprise the following steps：
Negative magnetic bead is added in biological specimen solution tank, above-mentioned negative magnetic bead is combined with antibody, above-mentioned biological specimen
Comprising tumour target cell and non-target cell to be captured, above-mentioned antibody is combined with non-target cell；Above-mentioned biological specimen is former blood
Liquid sample, above-mentioned antibody are CD45, and above-mentioned non-target cell includes red blood cell and leucocyte；
Capture magnetic head is placed in in the biological specimen that above-mentioned negative magnetic bead is combined, by the magnetic head in sample solution
Dead slow speed scanning motion, the non-target cell in sample solution is adsorbed onto on magnetic head, and is transferred in collection vessel；Thus,
Non-target cell in above-mentioned biological specimen separates with tumour target cell；In addition, in order to thoroughly remove the red blood cell in blood sample
And leucocyte, above-mentioned capture magnetic head can be repeatedly placed in biological specimen, repeatedly adsorb non-target cell, such as be chosen as 3
Secondary absorption；Above-mentioned capture magnetic head can catch the self-reacting devices such as instrument, Micron Technology Silicon Valley to realize the automation behaviour of capture magnet by magnetic
Make, manual operations also can be selected certainly.The cover that nonmagnetic substance can be selected is enclosed on above-mentioned capture magnetic head, avoids magnetic bead, non-
Target cell be captured magnetic head magnetic field absorption when directly contact on magnetic head, can so reach that can effectively to collect non-target thin
Born of the same parents, and the purpose of non-target cell is not damaged.
Next positive magnetic bead is added in the above-mentioned biological sample solution for removing non-target cell again, above-mentioned positive magnetic
Pearl is combined with antibody molecule EpCAM, at the same will capture magnetic head be placed in in above-mentioned biological specimen solution, by the magnetic head in sample
Dead slow speed scanning motion in solution, the tumour target cell in sample solution is adsorbed onto on magnetic head, and is transferred to collection vessel
In, it can be further used for the polygenes analysis of markers of target cell counting and unicellular or a small amount of cell.Positive enrichment with magnetic bead
To Magnetic Materials in free magnetic bead can be used filter remove.
The target cell that above-mentioned positive enrichment with magnetic bead obtains, DNA and RNA multiple gene mark by gene magnification, can be analyzed
The analysis of will thing, or directly carry out the analysis of the protein markers of cell, the analysis of eucaryotic cell structure and physical characteristic, external source body
Analysis of mark etc., as illustrated in figure 1 c.
Sample and negative magnetic bead mixed processing can be removed most of negative cells by the present embodiment after enrichment, then in this base
On plinth, positive enrichment target cell can not only improve detection target cell sensitivity, while target cell its purity that positive enrichment obtains
Also it can greatly improve, plus being counted to remaining cell after enrichment and genetic analysis, compensate for because positive magnetic bead antibody
Limitation, the analysis result of comprehensive positive enrichment with magnetic bead result and remaining cell, it is counted and cell analysis, genetic analysis knot
Fruit all more comprehensively, close to whole blood cell analysis.
Embodiment 2
As shown in Fig. 2 the cell enrichment separation method of the embodiment of the present invention 2, comprises the following steps：
Positive magnetic bead is added in biological specimen solution tank, above-mentioned positive magnetic bead is combined with antibody, above-mentioned biological specimen
Comprising tumour target cell and non-target cell to be captured, above-mentioned antibody is combined with tumour target cell；Above-mentioned biological specimen is original
Blood sample, above-mentioned antibody are that the above-mentioned non-target cells of Muc1 include red blood cell and leucocyte；
Capture magnetic head is placed in in the biological specimen that above-mentioned positive magnetic bead is combined, by the magnetic head in sample solution
Dead slow speed scanning motion, the tumour target cell in sample solution is adsorbed onto on magnetic head, and is transferred in collection vessel；Obtain
Target cell collect counting and carry out cell and genetic analysis.Thus, non-target cell and tumour in above-mentioned biological specimen
Target cell separates；Above-mentioned capture magnetic head can catch the self-reacting devices such as instrument, Micron Technology Silicon Valley to realize the automatic of capture magnet by magnetic
Change operation, manual operations also can be selected certainly.
Next negative magnetic bead is added in the above-mentioned biological sample solution for eliminating tumour target cell again, above-mentioned feminine gender
Magnetic bead is combined with antibody CD14, at the same will capture magnetic head be placed in in above-mentioned biological specimen solution, it is molten in sample by the magnetic head
Dead slow speed scanning motion in liquid, the non-target cell in sample solution is adsorbed onto on magnetic head, and is transferred in collection vessel, is removed
After removing most of non-target cell, remaining cell, which can be transferred to, carries out cell and genetic analysis on slide, can equally play
The effect of whole blood cell analysis detects.
Embodiment 3
The present embodiment is similar to Example 1, and difference is：Biological specimen is the blood sample of erythrocyte splitting；It is cloudy
Property the antibody that combines of magnetic bead be erythrocyte antibody (EA), the antibody of positive magnetic bead combination is keratin antibody.
Embodiment 4
The present embodiment is similar to Example 2, and difference is：Biological specimen is the blood sample or close of erythrocyte splitting
Spend gradient cell separating treatment and remove the blood sample after red blood cell.The antibody that negative magnetic bead combines is erythrocyte antibody (EA), positive
The antibody that magnetic bead combines is keratin antibody.
Comparative example 1
Negative magnetic bead is put into biological specimen groove, by capturing the multiple circulation absorption of magnetic head, to reach multiple the moon
Property enrichment processing, the magnetic material of the overwhelming majority can be eliminated, such as shown in Fig. 3 a-3d, after 3 negative enrichment processing,
More than 99.99% magnetic material is disposed of (shown in 3b-3d), in addition, after negative enrichment processing, the magnetic material in sample is basic
On the positive enrichment in later stage will not be had any impact.Repeatable self-reacting device can be used in the present embodiment to operate
Capture magnetic head is stated repeatedly to adsorb.
Comparative example 2
Fig. 4 a-4b are the situation schematic diagram of negative enrichment individual cells.It will be put with the good individual cells of negative marked by magnetic bead
Enter into different sample slots, by negative magnesphere, these single cells can be eliminated, and illustrate the present embodiment energy
Enough to remove even single non-specific cell, guarantee is not brought to follow-up processing step for example：In positive enrichment with magnetic bead, protect
The completeness of negative enrichment with magnetic bead is demonstrate,proved.
Comparative example 3
Fig. 5 a-5b are clear situation schematic diagram of the negative concentration method to the target cell of non-magnetic material.By negative magnetic bead and sun
Property mixing with cells, in principle, magnetic bead will not combine with positive cell.By negative magnesphere, most of magnetic can be removed
Pearl, but it is unclear unless the cell of this material.Thus, negative enrichment with magnetic bead only removes the cell and material that negative magnetic bead combines, and
Positive cell is not influenceed, negative concentration method is unclear except the target cell of non-magnetic material, as shown in Fig. 5 a-5b.
Technical scheme in above-mentioned the embodiment of the present application, at least has the following technical effect that or advantage：
Negative magnesphere and positive magnesphere are combined by the present invention, can first carry out negative magnetic to biological specimen
Negative enrichment is carried out again after positive enrichment with magnetic bead or positive enrichment with magnetic bead after pearl enrichment；Such as：The present invention is first by feminine gender
Magnetic bead and biological specimen are combined, and are combined the non-target cell in first order antibody and biological specimen on negative magnetic bead, then
Using magnetic head absorption non-target cell is captured, the non-target cell in biological specimen is separated with tumour target cell；Again by positive magnetic bead
It is added in the tumour target cell, after culture, positive magnetic bead will be combined with tumour target cell, and it is rich now to carry out positive magnetic bead
Collection, purer positive tumor target cell will be obtained.Therefore, the present invention can remove non-from a large amount of cells in biological specimen
Target cell, such as haemocyte (leucocyte and red blood cell), then separation and Extraction tumour cell therein, its background cells amount subtract significantly
Few, the purity of the tumour cell finally obtained improves first mate.Make follow-up cell and genetic analysis using such a product, will have
Beneficial to the analysis result that acquisition is optimal.Thus the inventive method improves target cell detection sensitivity, purity and the stabilization of detection
Property.For another example：Positive magnetic bead and biological specimen first can be combined by the present invention, be made in the antibody and biological specimen on positive magnetic bead
Tumour target cell be combined, recycle capture magnetic head absorption tumour target cell, by the non-target cell in biological specimen with it is swollen
Knurl target cell separates；Negative magnetic bead is added in the non-target cell again, negative magnetic bead will be combined with non-target cell, now
Negative enrichment with magnetic bead is carried out, obtains non-target cell.The target cell that above-mentioned positive enrichment obtains collects counting and carries out cell
And genetic analysis, the cell that feminine gender enrichment obtains can make cell and the genetic analysis of slide etc., it is thin can equally to play whole blood
Born of the same parents analyze the effect of detection.
Although preferred embodiments of the present invention have been described, but those skilled in the art once know basic creation
Property concept, then can make other change and modification to these embodiments.So appended claims be intended to be construed to include it is excellent
Select embodiment and fall into having altered and changing for the scope of the invention.Obviously, those skilled in the art can be to the present invention
Carry out various changes and modification without departing from the spirit and scope of the present invention.So, if these modifications and variations of the present invention
Belong within the scope of the claims in the present invention and its equivalent technologies, then the present invention is also intended to exist comprising these changes and modification
It is interior.
Claims (10)
- A kind of 1. cell enrichment separation method, it is characterised in that：Methods described includes：1), first kind magnetic bead and biological specimen are combined, the biological specimen includes first kind cell and the second class cell, The first kind magnetic bead is combined with first order antibody, by the first order antibody by the first kind cell and first kind magnetic bead It is combined；2), capture magnetic head is placed in in the biological specimen that the first kind magnetic bead is combined, the first kind cell absorption exists Capture on magnet arrangement；3), first kind cell is discharged from capture magnetic head assembly, removes the first kind cell in the biological specimen；4), the second class magnetic bead is combined with having removed the biological specimen of first kind cell, the second class magnetic bead is combined with Two antibody-likes, the second class cell is combined with the second class magnetic bead by the second order antibody；5), capture magnetic head is placed in in the biological specimen that the second class magnetic bead is combined, the second class cell absorption exists Capture on magnet arrangement；6), the second class cell is discharged from capture magnetic head assembly；When the first kind magnetic bead is negative magnetic bead, the second class magnetic bead is positive magnetic bead, and the first kind cell is non-target Cell, the second class cell are target cell.
- 2. cell enrichment separation method according to claim 1, it is characterised in that：It is in the step 3), the first kind is thin After born of the same parents discharge from capture magnetic head, the capture magnet is placed in in the biological specimen that the first kind magnetic bead is combined again, The first kind cell is adsorbed again.
- 3. cell enrichment separation method according to claim 1, it is characterised in that：It is in the step 6), the second class is thin After born of the same parents discharge from capture magnetic head, the capture magnet is placed in in the second class cell that second magnetic bead is combined again, The second class cell is adsorbed again.
- 4. cell enrichment separation method according to claim 1, it is characterised in that：The step 3) or 6) in, it is described to catch Obtain head surface and be provided with magnetosheath, the absorption of the described first or second class cell is on the magnetosheath of capture magnetic head assembly.
- 5. cell enrichment separation method according to claim 1, it is characterised in that：Methods described also includes：Will be through step 5) target cell that remaining cell and positive enrichment are collected into after capturing is collected on container or slide respectively, makees cell mirror Fixed, counting or gene recombination analysis.
- 6. cell enrichment separation method according to claim 1, it is characterised in that：Methods described also includes：By the target Cell is used for the polygenes analysis of markers of cell count and unicellular or a small amount of cell.
- 7. cell enrichment separation method according to claim 1, it is characterised in that：The biological specimen be blood sample or Biological fluid, the non-target cell include leucocyte and red blood cell, and the target cell includes tumour target cell.
- 8. cell enrichment separation method according to claim 7, it is characterised in that：The blood sample includes former blood sample Originally, the blood sample of erythrocyte splitting or density gradient cell separating treatment remove the blood sample after red blood cell.
- 9. cell enrichment separation method according to claim 1, it is characterised in that：Also include：The first kind magnetic bead is During positive magnetic bead, second magnetic bead is negative magnetic bead, and the first order antibody is that target cell identifies antibody, the secondary antibody Antibody is identified for non-target cell.
- 10. the cell enrichment separation method according to claim 1 or 9, it is characterised in that：The first order antibody is selected from CD45, CD14 and erythrocyte antibody (EA), the second order antibody are selected from EpCAM, Muc1 and keratin antibody.
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Cited By (4)
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| CN108865654A (en) * | 2018-06-29 | 2018-11-23 | 苏州百源基因技术有限公司 | A kind of cell sorting device and method for separating |
| CN108977404A (en) * | 2018-07-14 | 2018-12-11 | 北京航空航天大学青岛研究院 | Sizing screening method based on immunomagnetic beads label cell |
| CN109752530A (en) * | 2019-01-31 | 2019-05-14 | 宜昌美光硅谷生命科技股份有限公司 | A kind of liquid phase cellular immunity identification method and its application |
| CN112823202A (en) * | 2018-08-24 | 2021-05-18 | 深圳华大生命科学研究院 | Cell and biomolecule separation and extraction device and test system |
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