CN104833805A - Circulating tumor cell detection and identification kit and application thereof - Google Patents
Circulating tumor cell detection and identification kit and application thereof Download PDFInfo
- Publication number
- CN104833805A CN104833805A CN201510102012.XA CN201510102012A CN104833805A CN 104833805 A CN104833805 A CN 104833805A CN 201510102012 A CN201510102012 A CN 201510102012A CN 104833805 A CN104833805 A CN 104833805A
- Authority
- CN
- China
- Prior art keywords
- cell
- identification
- kit
- circulating tumor
- tumor cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a circulating tumor cell detection and identification kit and an application thereof and belongs to the field of biological and medical technology. The kit includes: a cell enrichment and separation and extraction reagent, a tumor cell identification reagent and a buffer solution. The kit is mainly characterized in that both a fluorescent staining marker based on fluorescent staining and cell morphology based on photopic vision dyeing can be observed at the same time, thereby increasing the accuracy rate of tumor cell identification. Through a cell surface marker and a cytoplasm marker for enriching targeted cells, the sensitivity of cell enrichment and separation and tumor cell extraction are increased. The kit is accurate in cell identification, is low in false positivity, is good in repeatability and is high in sensitivity, and is suitable for various applications in scientific research and clinical treatment.
Description
Technical field
The invention belongs to biotechnology and medical domain, be specifically related to a kind of circulating tumor cell identification kit and application thereof.
Background technology
Blood circulation tumour cell is present in blood with lower quantity, could effective determination and analysis after generally needing enrichment.Cell category in blood is various, the tumour cell in absolutely accurate ground qualification blood, and is not easy.Now, more generally, by the accreditation of professional domain, the tumour cell of qualification epithelial cell origin.The existing circulating tumor cell indentifying substance in market, be all separately for fluorescent dye or the dyeing of photopic vision light, cell enrichment methods is also the mark depending on cell surface.This invention is introduced and a kind ofly or can be carried out separately the circulating tumor cell indentifying substance of fluorescent dye observation and photopic vision light dyeing observation simultaneously, the mark used during cell enrichment comprises the mark of cell surface simultaneously and cytoplasmic mark, or only uses one wherein.
Summary of the invention
The object of the invention is to the deficiency for market today technical products, proposing one can multi-functional qualification blood circulation tumour cell, multiple markers enrich target cells, and improves the kit of the sensitivity that circulating tumor cell detects.
The technical solution used in the present invention is:
A kind of circulating tumor cell identification kit, described kit comprises erythrocyte lysing buffer, Premeabilisation of cells agent, cell enrichment reagents, cell fixative, cellular identification reagent and damping fluid and cell photopic vision light, fluorescent dyeing reagent and damping fluid, can carry out fluorescence and photopic vision light dyeing observation to the cell of same sample simultaneously.
The application of a kind of circulating tumor cell detection and identification kit, described kit can do fluorescence and the staining analysis of photopic vision light to cell simultaneously, cell surface marker and tenuigenin mark is relied on to carry out enrich target cells, high sensitivity enrichment and detection blood circulation tumour cell, may be used for the molecular marker of fluorescence labeling identification cell and the tumour cell of photopic vision light identification cellular prion protein.
Further, the reagent of the morphosis of described photopic vision light identification circulating tumor cell, comprises rare cell enrichment, separation and Extraction reagent, erythrocyte cracked liquid, Premeabilisation of cells damping fluid, cell immobile liquid, cleaning buffer solution, fluorescence labeling identification antibody and photopic vision light dyeing liquor etc.
Preferably, described rare cell enrichment, separation and Extraction reagent comprises antibody magnetic bead, dilution, the confining liquid of specific cells combination or claims embedding liquid, magnetic post and magnet arrangement.
Preferably, described erythrocyte cracked liquid is the red blood cell in lysed sample, and does not damage the reagent of other karyocytes.
Preferably, described Premeabilisation of cells damping fluid allows cell membrane carry out antibody infiltration but not ruptured cell.
Preferably, described cell immobile liquid refers to fixed cell, allows cell membrane have crack, is convenient to follow-up dyeing process.
Preferably, described cleaning buffer solution is for cleaning cell, changes different reagent treatment, but does not destroy cell.
Preferably, described fluorescence labeling identification antibody comprises the antibody reagent of the identification of cell of various mark.
Preferably, described photopic vision light dyeing liquor comprises dyeing liquor and the damping fluid of various optical effect.This
The beneficial effect of the invention is:
This reagent of the present invention is combined by fluorescent marker and photopic vision light observation eucaryotic cell structure, improves the degree of accuracy of qualification blood circulation tumour cell, obviously reduces the generation that false positive detects; By the method for cell surface marker and tenuigenin mark enrich target cells, significantly improve the sensitivity that circulating tumor cell detects, particularly successfully use multiple enriched antibody enrichment with magnetic bead target cell, significantly improve tumour cell detection sensitivity, simultaneously, on the basis of scientific research field and clinical test results, with immunological identification keratin, leukocyte common antigen (LCA) CD45, nucleus adds that cellular morphology dyeing reaches the object of the tumour cell effectively identifying epithelial cell origin in blood.This reagent manufacture compensate for market product weak point, an optimization, standardized kit, can with the use of the object of person, coordinate and select relevant market product, reach high rare cell enrichment, separation and Extraction efficiency, effectively identify the tumour cell of the epithelial origin in blood, marrow and body fluid, accuracy rate is high.Exist with general cellular identification reagent difference, except having fluorescent biological labels index, also have the cellular prion protein indexs such as photopic vision light dyeing as with reference to standard, significantly reduce false positive rate, improve the qualification accuracy rate of circulating tumor cell.And the operation steps of this kit is few, simple and convenient.
Accompanying drawing explanation
Fig. 1 uses the circulating tumor cell figure of fluorescence and the different cellular morphology of photopic vision light identification and analysis;
Fig. 2 uses the circulating tumor cell figure of fluorescence keratin expression different from photopic vision light identification and analysis;
Fig. 3 uses fluorescence and photopic vision light identification and analysis to differentiate single circulating tumor cell and multiple circulating tumor cell figure;
The identification and analysis figure of the tumour cell in Fig. 4 marrow.
Embodiment
Embodiment 1
Below in conjunction with accompanying drawing, the present invention is described further.
A kind of circulating tumor cell detects and identification kit, and kit comprises erythrocyte lysing buffer, Premeabilisation of cells agent, cell enrichment reagents, cell fixative, cellular identification reagent and damping fluid and cell photopic vision light, fluorescent dyeing reagent and damping fluid.The application of this circulating tumor cell identification kit, described kit can high sensitivity enrichment and detect blood circulation tumour cell, may be used for the molecular marker of fluorescence labeling identification cell and the tumour cell of photopic vision light identification cellular prion protein.The tumour cell of photopic vision light identification cellular prion protein comprises rare cell enrichment, separation and Extraction reagent, erythrocyte cracked liquid, Premeabilisation of cells damping fluid, cell immobile liquid, cleaning buffer solution, fluorescence labeling identification antibody and photopic vision light dyeing liquor etc.Rare cell enrichment, separation and Extraction reagent comprises antibody magnetic bead, dilution, confining liquid, magnetic post and the magnet arrangement that specific cells combines.Erythrocyte cracked liquid is the red blood cell in lysed sample, and does not damage the reagent of other karyocytes.Premeabilisation of cells damping fluid allows cell membrane have infiltration but not destroy cell.Cell immobile liquid refers to fixed cell, allows cell membrane have breach, is convenient to follow-up dyeing process.Cleaning buffer solution is for cleaning cell, changes different reagent treatment, but does not destroy cell.Fluorescence labeling identification antibody comprises the antibody reagent of the identification of cell of various mark.Photopic vision light dyeing liquor comprises various optical results dyeing liquor and cleaning buffer solution.
Cell image after dyeing as Figure 1-4.Fig. 1 is the rare tumour cell in the blood utilizing this kit to detect, applies the authentication method of this kit, from the blood cell of complexity, and the effectively tumour cell of qualification epithelial cell origin.Except having fluorescent marker keratin, haemocyte common antigen and nuclear Fluorescence Identification, the analysis of the light of photopic vision simultaneously shows complete, variform eucaryotic cell structure form.
Fig. 2 utilizes this kit, effectively detects the blood circulation tumour cell analyzing different keratin expression.
Fig. 3 utilizes this kit, effectively detects and analyzes single and multiple blood circulation tumour cells.
Fig. 4 utilizes this kit can rare tumour cell (keratin is positive, nucleus positive, and haemocyte common antigen is negative) effectively in identification and analysis marrow.
Embodiment 2
The detection of tumour cell and target cell qualification (referring to figs. 1 through Fig. 3) in breast cancer disease human blood
1. extract 10 milliliters of blood in EDTA heparin tube.Under this sample can be kept at 4 degree, 2 days effectively.
2. erythrocyte splitting process
2.1 regulate sample volume and erythrocyte cracked liquid volume, make final erythrocyte cracked liquid concentration be 1 times.
2.2 slight mixing samples incubated at room temperature 5-10 minute.
2.3 add PBS damping fluid to 50 milliliters, under room temperature, and centrifugal treating 700g 10 minutes.
2.4 carefully remove supernatant, do not lose the cell lump collected bottom test tube.
2.5 dilutions adding 450 millilambdas, slightly process, make cell become uniform cell suspending liquid soon.
3. target cell concentration
The 3.1 Premeabilisation of cells liquid respectively adding 100 millilambdas, cell immobile liquid, antibody magnetic bead, cell covering liquid.
After 3.2 abundant mixing, incubated at room temperature 45 minutes.
3.3 add dilution buffer to 10 milliliter, under room temperature, and centrifugal 300g 10 minutes.
3.4 use dilution, Eddy diffusion cell lump.
Above-mentioned cell suspending liquid is joined the magnetic post putting into magnetic field by 3.5, and by magnetic post.
3.6 wash a magnetic post with the dilution of 0.5 milliliter.
Magnetic bead is left magnetic field by 3.7, makes magnetic post discharge the cell combined.
4. sectioning cells
The cell obtained is passed through room temperature, 800rpm, the centrifugal treating of 3 minutes by 4.1, centrifugal on microslide.
4.2 removing supernatants, at centrifugal 1000rpm, 1 minute.
4.3 room temperatures or 37 degree of dry microslides 1 hour.
5. cell dyeing, qualification
5.1 fix above-mentioned microslide 10 minutes with immobile liquid.
Under 5.2 room temperatures, air oxygen detrition microslide 30 minutes.
5.3 clean microslide 1 time with cleaning buffer solution.
5.4 removing cleaning fluids, add fluorescent-labeled antibody dyeing liquor, dyeing 30-60 minute.
5.5 clean 1 time with cleaning buffer solution.
5.6, with photopic vision light dyeing liquor, dye and process.
5.7 clean 1 time with damping fluid.
5.8 use DAPI embedding medium mounting.
5.9 use fluorescence microscopy Analysis and Identification cell.
Embodiment 3
The detection of rare tumour cell and cellular identification (with reference to Fig. 4) in breast cancer patients marrow
1. bone marrow extraction liquid is in EDTA heparin tube, with the osteocomma of 70 millimicrons of filter screen filtration fragmentations.
2. erythrocyte splitting process
2.1 regulate sample volume and erythrocyte cracked liquid volume, make final erythrocyte cracked liquid concentration be 1 times.
2.2 slight mixing samples incubated at room temperature 5-10 minute.
2.3 add PBS damping fluid to 50 milliliters, under room temperature, and centrifugal treating 700g 10 minutes.
2.4 carefully remove supernatant, do not lose the cell lump collected bottom test tube.
2.5 dilutions adding 450 millilambdas, slightly process, make cell become uniform cell suspending liquid soon.
3. target cell concentration
The 3.1 Premeabilisation of cells liquid respectively adding 100 millilambdas, cell immobile liquid, antibody magnetic bead, cell covering liquid.
After 3.2 abundant mixing, incubated at room temperature 45 minutes.
3.3 add dilution buffer to 10 milliliter, under room temperature, and centrifugal 300g 10 minutes.
3.4 use dilution, Eddy diffusion cell lump.
Above-mentioned cell suspending liquid is joined the magnetic post putting into magnetic field by 3.5, and by magnetic post.
3.6 wash a magnetic post with the dilution of 0.5 milliliter.
Magnetic bead is left magnetic field by 3.7, makes magnetic post discharge the cell combined.
4. sectioning cells
The cell obtained is passed through room temperature, 800rpm, the centrifugal treating of 3 minutes by 4.1, centrifugal on microslide.
4.2 removing supernatants, at centrifugal 1000rpm, 1 minute.
4.2 room temperatures or 37 degree of dry microslides 1 hour.
5. cell dyeing, qualification
5.1 fix above-mentioned microslide 10 minutes with immobile liquid.
Under 5.2 room temperatures, air oxygen detrition microslide 30 minutes.
5.3 clean microslide 1 time with cleaning buffer solution.
5.4 removing cleaning fluids, add fluorescent-labeled antibody dyeing liquor, dyeing 30-60 minute.
5.5 clean 1 time with cleaning buffer solution.
5.6, with photopic vision light dyeing liquor, dye and process.
5.7 clean 1 time with damping fluid.
5.8 use DAPI embedding medium mounting.
5.9 use fluorescence microscopy Analysis and Identification cell.
This reagent of the present invention can be identified blood circulation tumour cell and improve the sensitivity of circulating tumor cell detection, particularly successfully with multiple enriched antibody enrichment with magnetic bead target cell, significantly improve tumour cell detection sensitivity, simultaneously, on the basis of scientific research field and clinical test results, with immunological identification keratin, leukocyte common antigen (LCA) CD45, nucleus adds that cellular morphology dyeing reaches, and effectively identifies the object of the tumour cell of epithelial cell origin in blood.Make up market product weak point, be the standardized kit of an optimization simultaneously, can with the use of the object of person, coordinate and select relevant market product, reach high rare cell enrichment, separation and Extraction efficiency, the tumour cell of the epithelial origin in effective qualification blood and marrow, accuracy rate is high.Exist with general cellular identification reagent difference, except having fluorescent biological labels index, also have the cellular prion protein indexs such as photopic vision light dyeing as with reference to standard, avoid false positive, improve accuracy rate.And the operation steps of this kit is few, simple and convenient.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (10)
1. a circulating tumor cell detects and identification kit, it is characterized in that, described kit comprises erythrocyte lysing buffer, Premeabilisation of cells agent, cell enrichment reagents, cell fixative, cellular identification reagent and damping fluid and cell photopic vision light staining reagent, fluorescent dyeing reagent and damping fluid, can carry out fluorescence and photopic vision light dyeing observation to the cell of same sample simultaneously.
2. the application of a circulating tumor cell detection according to claim 1 and identification kit, it is characterized in that, described kit can high sensitivity enrichment and detect blood circulation tumour cell or relevant target cells, may be used for the molecular marker of fluorescence labeling identification cell and the tumour cell of photopic vision light identification cellular prion protein.
3. the application of circulating tumor cell detection according to claim 2 and identification kit, it is characterized in that, can utilize cell surface and or cytoplasmic mark come enrichment, separation and extraction target cell, contained constituent comprises rare cell enrichment, separation and Extraction reagent, erythrocyte cracked liquid, Premeabilisation of cells damping fluid, cell immobile liquid, cleaning buffer solution, fluorescence labeling identification antibody and photopic vision light dyeing liquor etc.
4. the application of circulating tumor cell detection according to claim 3 and identification kit, it is characterized in that, described rare cell enrichment, separation and Extraction reagent comprises antibody magnetic bead, dilution, the confining liquid of specific cells combination or claims embedding liquid, magnetic post and magnet arrangement.
5. the application of circulating tumor cell detection according to claim 3 and identification kit, it is characterized in that, described erythrocyte cracked liquid is the red blood cell in lysed sample, and does not damage the reagent of other karyocytes.
6. circulating tumor cell according to claim 3 detects and the application of identification kit, it is characterized in that, the process of described Premeabilisation of cells damping fluid allows target cell membrane have to permeate antibody effect but not ruptured cell.
7. the application of circulating tumor cell detection according to claim 3 and identification kit, it is characterized in that, described cell immobile liquid refers to fixed cell, allows cell membrane have crack, is convenient to follow-up dyeing process.
8. circulating tumor cell according to claim 3 detects and the application of identification kit, and it is characterized in that, described cleaning buffer solution is for cleaning cell, changes different reagent treatment, but not ruptured cell.
9. circulating tumor cell according to claim 3 detects and the application of identification kit, it is characterized in that, described fluorescence labeling identification antibody comprise various fluorescence labeling, for the identification of the antibody reagent of cell.
10. the application of circulating tumor cell detection according to claim 3 and identification kit, it is characterized in that, described photopic vision light dyeing liquor comprise that various potential of hydrogen is debugged, there is specified chemical feature, do not affect fluorescent dye effect, the dyeing liquor with specific photopic vision light effect and cleaning buffer solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510102012.XA CN104833805A (en) | 2015-03-09 | 2015-03-09 | Circulating tumor cell detection and identification kit and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510102012.XA CN104833805A (en) | 2015-03-09 | 2015-03-09 | Circulating tumor cell detection and identification kit and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104833805A true CN104833805A (en) | 2015-08-12 |
Family
ID=53811817
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510102012.XA Pending CN104833805A (en) | 2015-03-09 | 2015-03-09 | Circulating tumor cell detection and identification kit and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104833805A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645726A (en) * | 2016-10-09 | 2017-05-10 | 天津普拉德生物科技有限公司 | Rapid detection kit for CTCs (circulating tumor cells) and preparation and application methods thereof |
| CN109752530A (en) * | 2019-01-31 | 2019-05-14 | 宜昌美光硅谷生命科技股份有限公司 | A kind of liquid phase cellular immunity identification method and its application |
| CN111024656A (en) * | 2019-09-10 | 2020-04-17 | 夏青青 | Cell autophagy staining detection kit and operation method thereof |
| CN111394416A (en) * | 2020-03-24 | 2020-07-10 | 三峡医学检验实验室(湖北)有限公司 | Cell liquid phase staining identification method with extremely small cell amount |
| CN113567672A (en) * | 2021-07-26 | 2021-10-29 | 江南大学附属医院 | Kit for detecting cancer cells in ascites or peritoneal lavage fluid |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101226118A (en) * | 2007-01-19 | 2008-07-23 | 中国医学科学院肿瘤研究所 | Cytochemical staining method being compatible with immunofluorescence analysis and uses thereof |
| CN101587043A (en) * | 2008-05-20 | 2009-11-25 | 林平 | Integrated method for enriching and detecting rare cell in biological fluid sample |
| CN102174465A (en) * | 2011-01-12 | 2011-09-07 | 武汉格蓝丽富科技有限公司 | Method for separating enriched target cells from tissues |
| CN102313813A (en) * | 2008-05-20 | 2012-01-11 | 北京莱尔生物医药科技有限公司 | Integration method for enriching and detecting rare cells from biological fluid samples |
| WO2012012803A2 (en) * | 2010-07-23 | 2012-01-26 | Advanced Cell Technology, Inc. | Methods for detection of rare subpopulations of cells and highly purified compositions of cells |
| CN102690786A (en) * | 2012-06-05 | 2012-09-26 | 武汉格蓝丽富科技有限公司 | Cell enriching, separating and extracting method and instrument and single cell analysis method |
| WO2014036951A1 (en) * | 2012-09-07 | 2014-03-13 | 中国科学院化学研究所 | Method and device for specifically capturing circulating tumor cell by utilizing surface of fractal structure and application thereof |
-
2015
- 2015-03-09 CN CN201510102012.XA patent/CN104833805A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101226118A (en) * | 2007-01-19 | 2008-07-23 | 中国医学科学院肿瘤研究所 | Cytochemical staining method being compatible with immunofluorescence analysis and uses thereof |
| CN101587043A (en) * | 2008-05-20 | 2009-11-25 | 林平 | Integrated method for enriching and detecting rare cell in biological fluid sample |
| CN102313813A (en) * | 2008-05-20 | 2012-01-11 | 北京莱尔生物医药科技有限公司 | Integration method for enriching and detecting rare cells from biological fluid samples |
| WO2012012803A2 (en) * | 2010-07-23 | 2012-01-26 | Advanced Cell Technology, Inc. | Methods for detection of rare subpopulations of cells and highly purified compositions of cells |
| CN102174465A (en) * | 2011-01-12 | 2011-09-07 | 武汉格蓝丽富科技有限公司 | Method for separating enriched target cells from tissues |
| CN102690786A (en) * | 2012-06-05 | 2012-09-26 | 武汉格蓝丽富科技有限公司 | Cell enriching, separating and extracting method and instrument and single cell analysis method |
| WO2014036951A1 (en) * | 2012-09-07 | 2014-03-13 | 中国科学院化学研究所 | Method and device for specifically capturing circulating tumor cell by utilizing surface of fractal structure and application thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645726A (en) * | 2016-10-09 | 2017-05-10 | 天津普拉德生物科技有限公司 | Rapid detection kit for CTCs (circulating tumor cells) and preparation and application methods thereof |
| CN109752530A (en) * | 2019-01-31 | 2019-05-14 | 宜昌美光硅谷生命科技股份有限公司 | A kind of liquid phase cellular immunity identification method and its application |
| CN111024656A (en) * | 2019-09-10 | 2020-04-17 | 夏青青 | Cell autophagy staining detection kit and operation method thereof |
| CN111394416A (en) * | 2020-03-24 | 2020-07-10 | 三峡医学检验实验室(湖北)有限公司 | Cell liquid phase staining identification method with extremely small cell amount |
| CN113567672A (en) * | 2021-07-26 | 2021-10-29 | 江南大学附属医院 | Kit for detecting cancer cells in ascites or peritoneal lavage fluid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Franquesa et al. | Update on controls for isolation and quantification methodology of extracellular vesicles derived from adipose tissue mesenchymal stem cells | |
| US11098274B2 (en) | Method and device for detecting circulating tumor cell | |
| CN101587043B (en) | Integrated method for enriching and detecting rare cell in biological fluid sample | |
| CN104007257B (en) | Method for detecting non-humoral rare karyotes, and kit thereof | |
| CN105954246B (en) | Method and kit for detecting free rare tumor cells in human biological fluid sample | |
| CN105785005A (en) | Circulating tumor cell detection kit and application thereof | |
| CN104833805A (en) | Circulating tumor cell detection and identification kit and application thereof | |
| US11969739B2 (en) | Apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells | |
| CN108698846A (en) | The composition and method of rare cells for identification | |
| JP2016527907A (en) | Selective delivery of substances to cells | |
| CN109187146B (en) | Human body cell full-form immunofluorescence staining method and kit | |
| CN106399250A (en) | Method and kit for separating exosome | |
| Kim et al. | A microchip filter device incorporating slit arrays and 3-D flow for detection of circulating tumor cells using CAV1-EpCAM conjugated microbeads | |
| CN103031276A (en) | Method for obtaining circulating tumor single-cell | |
| US11371982B2 (en) | Method of predicting patient prognosis using rare cells | |
| CN106970225B (en) | A kind of kit and its application for combining 8 probe identification circulating tumor cells of CEP using CD45 immunofluorescences | |
| CN110431423A (en) | Suffer from the method for cancer object Overall survival and progression free survival phase using circulation cancer associated macrophages like cell (CAML) prediction | |
| JP2020030180A (en) | Hemolytic agent for blood sample | |
| CN102313813B (en) | Integration method for enriching and detecting rare cells from biological fluid samples | |
| US11135598B2 (en) | Apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells | |
| CN102174465A (en) | Method for separating enriched target cells from tissues | |
| CN111596053B (en) | Application of TPN molecules in preparation of circulating tumor cell detection reagent, detection reagent and kit | |
| WO2017149564A1 (en) | Method for detecting or separating/obtaining circulating tumor cell employing cell proliferation method | |
| US11241699B2 (en) | Apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells | |
| JP2019056678A (en) | Target cell detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150812 |