CN105475121B - A kind of selection of high temperature resistant disease resisting rice two-line sterile line - Google Patents
A kind of selection of high temperature resistant disease resisting rice two-line sterile line Download PDFInfo
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- CN105475121B CN105475121B CN201610067488.9A CN201610067488A CN105475121B CN 105475121 B CN105475121 B CN 105475121B CN 201610067488 A CN201610067488 A CN 201610067488A CN 105475121 B CN105475121 B CN 105475121B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of selection of high temperature resistant disease resisting rice two-line sterile line, step is：1) it is female parent with two-line sterile line of rice S, the glutinous farm variety C of high temperature resistant round-grained rice is paternal hybrid, is indicated with S × C；2) plantation of the 2nd season is female parent with S × C, with the three of blast resisting and false smut be maintainer B is that male parent is reestablished diplomatic relations, and harvest is reestablished diplomatic relations seed (S × C) × B after reestablishing diplomatic relations；3) reestablish diplomatic relations Offspring F2-F4；4) reestablish diplomatic relations offspring F5, and hybridizing preparation test cross for two-line sterile line single plant with F5 combines；Rice pocket is cut after test cross sowing adds generation；F6 is for Single-plant selection and the same F2-F4 of Jia Dai methods；5) reestablish diplomatic relations offspring F7, and the two-line sterile line of choosing is stayed to finalize the design almost.It can be selected using this method solid preferable under high temperature, and the two-line sterile line of rice of energy blast resisting and false smut, it is the low disadvantage with disease resistance difference of rice varieties high temperature setting percentage that existing two, which can be improved, is conducive to the double-linear hybrid rice kind for cultivating stable high yield.
Description
Technical field
The invention belongs to agricultural breeding field, " two line method " Parents In Hybrid Rice selection and breeding are may be directly applied to.
Background technology
Currently, " two line method " hybrid rice variety is quickly grown in China middle and lower reach of Yangtze River basin, in the ecological region planting face
Product has been more than " three line method " hybrid rice variety.But in Upper Yangtze River high temperature dry season region, " two line method " hybrid rice variety institute
Accounting example is also small compared with " three line method " cenospecies, this is primarily due to existing " two systems " hybrid rice variety high temperature resistant and disease resistance
Also it is not very strong, constrains the further development of double-linear hybrid rice kind.The high temperature resistant and disease resistance of hybrid rice variety
Related with parent, parent directly determines the high temperature resistant of Hybrid and disease-resistant level.
Existing two-line sterile line deposits defect both ways：First, it is that existing two-line sterile line heat-resisting ability is poor, causes
High temperature dry season area's hybrid rice variety setting percentage is low, influences hybrid yield；Second is existing two-line sterile line blast resisting
It is weak with false smut ability, gently then cause the rice underproduction, it is heavy then have no harvest.
Therefore, the high temperature resistant of double-linear hybrid rice kind can be improved by cultivating high temperature resistant and disease-resistant two-line sterile line
And disease resistance.
Invention content
Place in view of the above-mentioned deficiencies in the prior art, the present invention provides a kind of two systems of high temperature resistant disease resisting rice not
Educate the selection for being.
In order to solve the above-mentioned technical problem, present invention employs following technical solutions：
A kind of selection of high temperature resistant disease resisting rice two-line sterile line, this method comprises the following steps：
1) it is female parent with existing two-line sterile line of rice S, the glutinous farm variety C of high temperature resistant round-grained rice is paternal hybrid, obtains F1 generation,
It is indicated with S × C；
2) plantation of the 2nd season is female parent with S × C, with the three of blast resisting and false smut be maintainer B is that male parent is reestablished diplomatic relations, multiple
Seed (S × C) × B that reestablishes diplomatic relations is harvested after friendship；
3) reestablishing diplomatic relations, Offspring F2-F4 is in Hainan and Chongqing rice disease identification base is shuttled plus generation；It reflects in Chongqing rice disease
Determine base add for when selection economical character is good, Anther Abortion thoroughly, do not send out rice blast and the single plant of false smut, remove the filling of rice pocket cold water
It irrigates and adds generation；Hainan add for when, sowed in early or mid November, according to fertility-sensitive period mean temperature and control infertility after beginning next year fringe
Line fertility chooses good economical character, reasonable anther fertility and the single plant bagging that do not fall ill, it is ripe after sowing；
4) the offspring F5 that reestablishes diplomatic relations starts in Chongqing positive season to be test cross male parent with self-fertile two-line sterile line R26, not with F5 two systems of generation
Educate in based material economical character is good, Anther Abortion thoroughly, the single plant hybridization of not sending out rice blast and false smut prepare test cross combination；It surveys
Rice pocket, which is cut, after friendship combination sowing adds generation；Single-plant selection and Jia Dai method same F2-F4 of the F6 generations in Hainan；
5) the offspring F7 that reestablishes diplomatic relations starts, respectively in the positive season rice disease identification base in Chongqing and high temperature dry season breed of variety base
1 year is planted in the two-line sterile line test cross combination prepared in Chongqing positive season；After maturation, the rate schedule of test cross combination is counted respectively
Existing, rice blast and false smut incidence eliminate corresponding two-line sterile line；At this point, the two-line sterile line of choosing is stayed to finalize the design almost.
The beneficial effects of the invention are as follows：Using this method can select under high temperature it is solid preferably, and can blast resisting and
The two-line sterile line of rice of false smut, made of the sterile line maternal preparation Hybrid Rice Combinations also have the advantages that it is above-mentioned, can be with
It is the low disadvantage with disease resistance difference of rice varieties high temperature setting percentage to improve existing two, is conducive to rice high yield stable yields.
Description of the drawings
Fig. 1 is a kind of flow chart of the selection of high temperature resistant disease resisting rice two-line sterile line.
Specific implementation mode
A kind of selection of high temperature resistant disease resisting rice two-line sterile line, flow is as shown in Figure 1, this method includes as follows
Step：
1) it is female parent with existing two-line sterile line of rice S, the glutinous farm variety C of high temperature resistant round-grained rice is paternal hybrid, obtains F1 generation,
It is indicated with S × C；
2) plantation of the 2nd season is female parent with S × C, with the three of blast resisting and false smut be maintainer B is that male parent is reestablished diplomatic relations, multiple
Seed (S × C) × B that reestablishes diplomatic relations is harvested after friendship；
3) reestablishing diplomatic relations, Offspring F2-F4 is in Hainan and Chongqing rice disease identification base is shuttled plus generation；In Chongqing anti-disease enzyme base
Ground add for when selection economical character is good, Anther Abortion thoroughly, do not send out rice blast and the single plant of false smut, remove rice pocket cold water irrigation and add
Generation；Hainan plus for when, sowed in early or mid November, according to fertility-sensitive period mean temperature and control sterile line after beginning next year fringe
Fertility chooses good economical character, reasonable anther fertility and the single plant bagging that do not fall ill, it is ripe after sowing；
4) the offspring F5 that reestablishes diplomatic relations starts in Chongqing positive season to be test cross male parent with self-fertile two-line sterile line R26, not with F5 two systems of generation
Educate in based material economical character is good, Anther Abortion thoroughly, the single plant hybridization of not sending out rice blast and false smut prepare test cross combination；It surveys
Rice pocket, which is cut, after acceptance kind adds generation；Single-plant selection and Jia Dai method same F2-F4 of the F6 generations in Hainan；
5) the offspring F7 that reestablishes diplomatic relations starts, respectively in the positive season rice disease identification base in Chongqing and high temperature dry season breed of variety base
1 year is planted in the two-line sterile line test cross combination prepared in Chongqing positive season；After maturation, the rate schedule of test cross combination is counted respectively
Existing, rice blast and false smut incidence eliminate corresponding two-line sterile line；At this point, the two-line sterile line of choosing is stayed to finalize the design almost.
Embodiment
It is maternal (S), the glutinous agriculture of the heat-resisting round-grained rice in Chongqing that in July, 2010 extensively accounts for 63-4S in Chongqing positive season with two-line sterile line
Family's kind " long-lived glutinous rice " (C) combines for male parent preparing hybrid, and this season harvests 25, F1 (S × C) seed.In September, 2010 is in sea
Nanling water sowing F1 (S × C) 25 transplants 20 plants after seedling.With F1 (S × C) to be maternal after beginning fringe, self-fertile blast resisting and rice
The three of bent disease are that long-grained nonglutinous rice maintainer Q6A (B) reestablishes diplomatic relations for male parent, and this season harvests seed of reestablishing diplomatic relations【(S×C)×B】30.
30 seeds of reestablishing diplomatic relations are sowed in March, 2011 Chongqing Resistance screening base, transplant 20 plants, choose that hybrid vigour is strong, agriculture
3 good, disease-free single plants of skill character are mixed to receive 2100, F1 generation seed.In F2 generations, are transplanted into 200 rows in Hainan in by the end of November, 2011,
10 caves/row, while transplanting Y58S and depth 08S and being compareed as fertility.When reinstating instrument record proving ground on January 1st, 2012 per
Temperature.After on March 15th, 2012 F2 groups beginning fringe, it is good that by cave economical character is selected to Y58S, every 1 time on the 3rd of depth 08S and F2 groups
Single plant list fringe bagging is selfed, and number and bagging date are indicated on sack, until March 27 is only.
Sack and tassel are cut and bring back laboratory by April 20th, 2012.Statistics 4 bagging Y58S's and depth 08S is solid
Rate, it is F3 generations to choose F2 strain sowing of the bagging same day setting percentage less than Y58S and depth 08S and retain, and eliminates the high single plant of setting percentage
Tassel.
F3 seeds and Y58S, depth 08S are seeded in Chongqing rice disease and identify base by May 1st, 2012, are pressed May 20
The single transplantation of seedlings of 10 caves/row, other same crop fields of management measure.Into after boot stage, 2 Chongqing locality rice blast and false smut are sprayed
Spore cultivation liquid induces rice blast and false smut.The selection economical character of August 18 days is good, Anther Abortion thoroughly, do not send out rice blast and
The single plant of false smut, removes F3 and control rice pocket cold water irrigation adds generation, records per daily mean temperature.By rice pocket seed before October 25
F4 has been withheld, and eliminates the single plant that setting percentage is higher than Y58S and depth 08S.
F4 seeds are seeded in Hainan by November 15th, 2012, obtain F5 generations in same way as described above.
F5 seeds are seeded in Chongqing rice disease and identify base by April 29th, 2013, are chosen after entering beginning ear period by the end of June
Economical character is good, Anther Abortion is thorough, does not send out rice blast and the single plant R26 test crosses of false smut.After test cross combination sowing immediately
Menu strain in resettlement, with cold water irrigation plus for acquisition F6.Add for the same F2-F4 of method.
On November 17th, 2013 sows F6 in Hainan, is added with F2-F4 single plants and obtains F7 generations for method.
On March 12nd, 2014 sows the test cross prepared with R26 the half 6-7 months in 2013 in Chongqing rice disease identification base
Seed is combined, while sowing remaining half test cross in high temperature dry season breed of variety base and combining seed.In by the end of April, 2014 in rice
F7 is sowed for two-line sterile line material in Disease identification base.
August identifies base and high temperature dry season breed of variety base investigation statistics in -6 days on the 5th in rice disease respectively within 2014
Breeding time, rice blast, false smut incidence, setting percentage and the economic flow rate (table 1) of the 20 caves R26 test cross combinations prepared.It washes in a pan
Eliminate that 50% or more rice blast incidence, 30% or more false smut incidence, 60% or less setting percentage, economic flow rate be low, breeding time
Long test cross combines corresponding two-line sterile line material.Remaining two-line sterile line selects sterile line, material shaping in being.
Table 1:20 cave test crosses combine statistical data
Using above method success selection and breeding Q1S, which has that big fringe, moderate growth duration, disease resistance be strong, agronomy
Shape is good, has the features such as higher coordinate force with R26.
Finally illustrate, the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although with reference to compared with
Good embodiment describes the invention in detail, it will be understood by those of ordinary skill in the art that, it can be to the skill of the present invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the right of invention.
Claims (1)
1. a kind of selection of high temperature resistant disease resisting rice two-line sterile line, which is characterized in that this method comprises the following steps：
1) it is female parent with existing two-line sterile line of rice S, the glutinous farm variety C of high temperature resistant round-grained rice is paternal hybrid, obtains F1 generation, uses S
× C is indicated；
2) plantation of the 2nd season is female parent with S × C, with the three of blast resisting and false smut be maintainer B is that male parent is reestablished diplomatic relations, after reestablishing diplomatic relations
Harvest seed (S × C) × B that reestablishes diplomatic relations；
3) reestablishing diplomatic relations, Offspring F2-F4 is in Hainan and Chongqing rice disease identification base is shuttled plus generation；In Chongqing, rice disease identifies base
Ground add for when selection economical character is good, Anther Abortion thoroughly, do not send out rice blast and the single plant of false smut, remove rice pocket cold water irrigation and add
Generation；Hainan add for when, sowed in early or mid November, according to mean temperature when fertility-sensitive period and control sterile line after beginning next year fringe
Fertility chooses good economical character, reasonable anther fertility and the single plant bagging that do not fall ill, it is ripe after sowing；
4) the offspring F5 that reestablishes diplomatic relations starts in Chongqing positive season to be test cross male parent with self-fertile two-line sterile line R26, with F5 for two-line sterile line
Economical character is good in material, Anther Abortion is thorough, do not send out rice blast and test cross combination is prepared in the hybridization of the single plant of false smut；Hybridization group
Rice pocket, which is cut, after conjunction sowing adds generation；In F6 generations, are in the Single-plant selection in Hainan and the Single-plant selection of the same F2-F4 of Jia Dai methods and plus Dai Fang
Method；
5) the offspring F7 that reestablishes diplomatic relations starts, and is planted respectively in the positive season rice disease identification base in Chongqing and high temperature dry season breed of variety base
Upper one year is in the two-line sterile line test cross combination prepared in Chongqing positive season；After maturation, yield performance, the rice of test cross combination are counted respectively
Seasonal febrile diseases and false smut incidence eliminate corresponding two-line sterile line；At this point, the two-line sterile line of choosing is stayed to shape.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105918110A (en) * | 2016-05-10 | 2016-09-07 | 江苏农林职业技术学院 | Cultivation method for aromatic high-quality rice sterile line |
| CN106332776A (en) * | 2016-11-16 | 2017-01-18 | 重庆市农业科学院 | Method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice |
| CN106665365B (en) * | 2017-02-24 | 2018-08-07 | 吉林省寒土地农业科技有限公司 | A kind of heat-resisting Japonica two line hybrid rice new method |
| CN108967187A (en) * | 2018-09-29 | 2018-12-11 | 江西省超级水稻研究发展中心 | A kind of rice chalky grain rate high temperature insensitiveness germplasm selection |
| CN111436365B (en) * | 2020-03-25 | 2023-08-18 | 上海天谷生物科技股份有限公司 | Cultivation method of water-saving drought-resistant rice two-line sterile line |
| CN113229140A (en) * | 2021-06-04 | 2021-08-10 | 广西星霞农业科技有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant two-line sterile line of rice |
| CN115176699A (en) * | 2022-08-01 | 2022-10-14 | 湖南仕叶生态农业科技发展有限公司 | Rice cultivation technology |
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| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
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| CN104839010A (en) * | 2015-05-21 | 2015-08-19 | 福建金山都发展有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line |
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Patent Citations (3)
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| CN102405827A (en) * | 2011-08-16 | 2012-04-11 | 连云港市农业科学院 | Rapid breeding method of waxy rice dual-purpose genic male sterile line |
| CN104663423A (en) * | 2015-02-05 | 2015-06-03 | 广德县农业科学研究所 | Method for breeding thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S |
| CN104839010A (en) * | 2015-05-21 | 2015-08-19 | 福建金山都发展有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line |
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