CN105475121A - Breeding method for high-temperature-resistant and disease-resistant rice two-line sterile line - Google Patents
Breeding method for high-temperature-resistant and disease-resistant rice two-line sterile line Download PDFInfo
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- CN105475121A CN105475121A CN201610067488.9A CN201610067488A CN105475121A CN 105475121 A CN105475121 A CN 105475121A CN 201610067488 A CN201610067488 A CN 201610067488A CN 105475121 A CN105475121 A CN 105475121A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a breeding method for a high-temperature-resistant and disease-resistant rice two-line sterile line. The breeding method includes the steps that the rice two-line sterile line S is used as a female parent, a high-temperature-resistant glutinous rice farm variety C is used as a male parent and hybridized with the female parent, and the generation F1 is expressed through S*C; the generation S*C is used as a female parent during planting in the second season, a three-line maintainer line B resistant to rice blast and false smut is used as a male parent and is subjected to composite hybridization with the female parent, and composite hybridization seeds (S*C)*B are harvested after composite hybridization; the generations F2-F4 are subjected to composite hybridization; the generation F5 is subjected to composite hybridization, and a generation F5 two-line sterile line single plant is hybridized to prepare a hybridization testing combination; after hybridization testing seeds are harvested, rice bags are cut for generation adding; the single plant selecting and generation adding method of the generation F6 is the same as that of the generations F2-F4; the generation F7 is subjected to composite hybridization, and the selected two-line sterile line is basically set. The rice two-line sterile line capable of seeding well at high temperature and capable of resisting rice blast and false smut can be bred through the method, the defects that an existing two-line rice variety is low in high-temperature seeding rate and poor in disease resistance can be overcome, and a high and stable yield two-line hybrid rice variety can be cultivated easily.
Description
Technical field
The invention belongs to agricultural breeding field, the seed selection of " two line method " Parents In Hybrid Rice can be directly applied to.
Background technology
At present, " two line method " hybrid rice variety in the development of basin, China middle and lower reach of Yangtze River rapidly, has exceeded " three line method " hybrid rice variety at this ecological region planting area.But in Upper Yangtze River high temperature dry season region, " two line method " hybrid rice variety proportion is also little compared with " three line method " crossbreed, this mainly because existing " two are " hybrid rice variety is high temperature resistant and resistance against diseases is not also very strong, constrains further developing of double-linear hybrid rice kind.Hybrid rice variety high temperature resistant relevant with parent with resistance against diseases, parent directly determines the high temperature resistant and disease-resistant level of Hybrid.
Existing two-line sterile line deposits defect both ways: the first, is that existing two-line sterile line heat-resisting ability is poor, causes at high temperature dry season district's hybrid rice variety ripening rate low, affects hybrid yield; Second is that existing two-line sterile line blast resisting and false smut ability are weak, gently then causes the paddy rice underproduction, heavy then have no harvest.
Therefore, the high temperature resistant of double-linear hybrid rice kind and resistance against diseases can be improved by cultivating high temperature resistant and disease-resistant two-line sterile line.
Summary of the invention
For above-mentioned the deficiencies in the prior art part, the invention provides a kind of selection of high temperature resistant disease resisting rice two-line sterile line.
In order to solve the problems of the technologies described above, present invention employs following technical scheme:
A selection for high temperature resistant disease resisting rice two-line sterile line, the method comprises the steps:
1) with existing two-line sterile line of rice S for female parent, the glutinous farm variety C of high temperature resistant round-grained rice is paternal hybrid, obtain F1 generation, represent with S × C;
2) plantation of the 2nd season be maternal with S × C, be maintainer B is that male parent is reestablished diplomatic relations by three of blast resisting and false smut, gathers in the crops seed (S × C) × B that reestablishes diplomatic relations after reestablishing diplomatic relations;
3) Offspring F2-F4 that reestablishes diplomatic relations shuttles back and forth in Hainan and Chongqing rice disease qualification base and adds generation; Add in Chongqing rice disease qualification base for time select that economical character is good, individual plant that Anther Abortion thoroughly, does not send out rice blast and false smut, remove rice pocket cold water irrigation and add generation; Hainan add for time, in early or mid November sowing, according to the individual plant bagging that fertility-sensitive period mean temperature and contrast male sterile line fertility are chosen good, the reasonable flower pesticide fertility of economical character and do not fallen ill after beginning next year fringe, sowing after ripe;
4) it is test cross male parent that the offspring F5 that reestablishes diplomatic relations starts the positive season self-fertile two-line sterile line R26 in Chongqing, hybridizes prepare test cross and combine with the individual plant that F5 is good for economical character in two-line sterile line material, Anther Abortion thoroughly, does not send out rice blast and false smut; Cut rice pocket after test cross combination sowing and add generation; F6 is for the Single-plant selection in Hainan and the same F2-F4 of Jia Dai method;
5) the offspring F7 that reestablishes diplomatic relations starts, the two-line sterile line test cross combination of positive season rice disease qualification base and high temperature dry season breed of variety base plantation upper one year of positive season preparation in Chongqing in Chongqing respectively; After maturation, add up the output performance of test cross combination, rice blast and the false smut incidence of disease respectively, eliminate corresponding two-line sterile line; Now, the two-line sterile line of choosing is stayed to finalize the design almost.
The invention has the beneficial effects as follows: better solid under adopting this method can select high temperature, and the two-line sterile line of rice of energy blast resisting and false smut, the Hybrid Rice Combinations making maternal preparation of this male sterile line also has above-mentioned advantage, the shortcoming that existing two are the low and disease resistance difference of rice varieties high temperature ripening rate can be improved, be conducive to rice high yield stable yields.
Accompanying drawing explanation
Fig. 1 is a kind of flow chart of selection of high temperature resistant disease resisting rice two-line sterile line.
Embodiment
A selection for high temperature resistant disease resisting rice two-line sterile line, as shown in Figure 1, the method comprises the steps: its flow process
1) with existing two-line sterile line of rice S for female parent, the glutinous farm variety C of high temperature resistant round-grained rice is paternal hybrid, obtain F1 generation, represent with S × C;
2) plantation of the 2nd season be maternal with S × C, be maintainer B is that male parent is reestablished diplomatic relations by three of blast resisting and false smut, gathers in the crops seed (S × C) × B that reestablishes diplomatic relations after reestablishing diplomatic relations;
3) Offspring F2-F4 that reestablishes diplomatic relations shuttles back and forth in Hainan and Chongqing rice disease qualification base and adds generation; Add in anti-disease enzyme base, Chongqing for time select that economical character is good, individual plant that Anther Abortion thoroughly, does not send out rice blast and false smut, remove rice pocket cold water irrigation and add generation; Add in Hainan for time, in early or mid November sowing, according to the individual plant bagging that fertility-sensitive period mean temperature and contrast male sterile line fertility are chosen good, the reasonable flower pesticide fertility of economical character and do not fallen ill after beginning next year fringe, sowing after ripe;
4) it is test cross male parent that the offspring F5 that reestablishes diplomatic relations starts the positive season self-fertile two-line sterile line R26 in Chongqing, hybridizes prepare test cross and combine with the individual plant that F5 is good for economical character in two-line sterile line material, Anther Abortion thoroughly, does not send out rice blast and false smut; Cut rice pocket after test cross sowing and add generation; F6 is for the Single-plant selection in Hainan and the same F2-F4 of Jia Dai method;
5) the offspring F7 that reestablishes diplomatic relations starts, the two-line sterile line test cross combination of positive season rice disease qualification base and high temperature dry season breed of variety base plantation upper one year of positive season preparation in Chongqing in Chongqing respectively; After maturation, add up the output performance of test cross combination, rice blast and the false smut incidence of disease respectively, eliminate corresponding two-line sterile line; Now, the two-line sterile line of choosing is stayed to finalize the design almost.
Embodiment
In July, 2010, positive season extensively accounted for 63-4S for maternal (S) with two-line sterile line in Chongqing, the glutinous farm variety of the heat-resisting round-grained rice in Chongqing " long-lived glutinous rice " (C) is the combination of male parent preparing hybrid, this season results 25, F1 (S × C) seed.In September, 2010, at Lingshui, Hainan sowing F1 (S × C) 25, transplants 20 strains after seedling.Be maternal with F1 (S × C) after beginning fringe, three of self-fertile blast resisting and false smut is long-grained nonglutinous rice maintainer Q6A (B) for male parent is reestablished diplomatic relations, and this season results are reestablished diplomatic relations 30, seed [(S × C) × B].
30 seeds of reestablishing diplomatic relations are sowed in March, 2011 Chongqing Resistance screening base, transplant 20 strains, choose that hybrid vigour is strong, economical character good, disease-free 3 individual plants are mixed receives 2100, F1 generation seed.In Hainan, in F2 generation, is transplanted 200 row in by the end of November, 2011,10 caves/OK, transplant Y58S and dark 08S simultaneously and contrast as fertility.Reinstate on January 1st, 2012 instrument record proving ground per time temperature.After on March 15th, 2012 F2 colony beginning fringe, by cave, the selfing of economical character good individual plant list fringe bagging is selected 1 time on the every 3rd to Y58S, dark 08S and F2 colony, sack indicates numbering and bagging date, stopped to March 27.
Sack and tassel are cut and are brought back laboratory by April 20th, 2012.Add up the ripening rate of 4 bagging Y58S and dark 08S, choose bagging ripening rate on the same day lower than the F2 strain sowing retention of Y58S and dark 08S and F3 generation, eliminate the individual plant tassel that ripening rate is high.
F3 seed and Y58S, dark 08S are seeded in rice disease qualification base, Chongqing by May 1st, 2012, and May 20 pressed the single transplantation of seedlings of 10 cave/row, the same land for growing field crops of other control measures.After entering booting stage, spray 2 local rice blast in Chongqing and false smut Spore cultivation liquid, bring out rice blast and false smut.August 18 selected the individual plant that economical character is good, Anther Abortion thoroughly, does not send out rice blast and false smut, removed F3 and contrast rice pocket cold water irrigation to add generation, recorded every daily mean temperature.Before October 25, rice pocket seed F4 was withheld, eliminated the individual plant of ripening rate higher than Y58S and dark 08S.
F4 planting seed in Hainan, is obtained F5 generation by above-mentioned same method by November 15th, 2012.
F5 planting seed in Chongqing rice disease qualification base, is entered beginning fringe after date and chooses the individual plant R26 test cross that economical character is good, Anther Abortion thoroughly, does not send out rice blast and false smut by April 29th, 2013 by the end of June.Menu strain in resettlement immediately after test cross combination sowing, adds generation acquisition F6 with cold water irrigation.Add for the same F2-F4 of method.
On November 17th, 2013, at Hainan sowing F6, adds with F2-F4 individual plant and obtains F7 generation for method.
The test cross combination seed that on March 12nd, 2014 prepared with R26 in the Chongqing rice disease qualification base sowing half 6-7 month in 2013, simultaneously at high temperature dry season breed of variety base sowing residue half test cross combination seed.F7 is sowed for two-line sterile line material in by the end of April, 2014 in rice disease qualification base.
The breeding time of combining with the test cross that R26 prepares in rice disease qualification base and investigation statistics 20 cave, high temperature dry season breed of variety base respectively-6 days on the 5th August in 2014, rice blast, the false smut incidence of disease, ripening rate and economic flow rate (table 1).The superseded rice blast incidence of disease more than 50%, the false smut incidence of disease more than 30%, ripening rate less than 60%, the test cross that economic flow rate is low, breeding time is long combine corresponding two-line sterile line material.Residue two-line sterile line selects male sterile line, material shaping in being namely.
Table 1:20 cave test cross combination statistical data
Utilize said method success seed selection Q1S, this male sterile line has large fringe, moderate growth duration, disease resistance is strong, economical character good, have the features such as higher coordinate force with R26.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (1)
1. a selection for high temperature resistant disease resisting rice two-line sterile line, it is characterized in that, the method comprises the steps:
1) with existing two-line sterile line of rice S for female parent, the glutinous farm variety C of high temperature resistant round-grained rice is paternal hybrid, obtain F1 generation, represent with S × C;
2) plantation of the 2nd season be maternal with S × C, be maintainer B is that male parent is reestablished diplomatic relations by three of blast resisting and false smut, gathers in the crops seed (S × C) × B that reestablishes diplomatic relations after reestablishing diplomatic relations;
3) Offspring F2-F4 that reestablishes diplomatic relations shuttles back and forth in Hainan and Chongqing rice disease qualification base and adds generation; Add in Chongqing rice disease qualification base for time select that economical character is good, individual plant that Anther Abortion thoroughly, does not send out rice blast and false smut, remove rice pocket cold water irrigation and add generation; Hainan add for time, in early or mid November sowing, according to the individual plant bagging that mean temperature during fertility-sensitive period and contrast male sterile line fertility are chosen good, the reasonable flower pesticide fertility of economical character and do not fallen ill after beginning next year fringe, sowing after ripe;
4) it is test cross male parent that the offspring F5 that reestablishes diplomatic relations starts the positive season self-fertile two-line sterile line R26 in Chongqing, hybridizes prepare test cross and combine with the individual plant that F5 is good for economical character in two-line sterile line material, Anther Abortion thoroughly, does not send out rice blast and false smut; Cut rice pocket after hybrid combination sowing and add generation; F6 is for the Single-plant selection in Hainan and the same F2-F4 of Jia Dai method;
5) the offspring F7 that reestablishes diplomatic relations starts, the two-line sterile line test cross combination of positive season rice disease qualification base and high temperature dry season breed of variety base plantation upper one year of positive season preparation in Chongqing in Chongqing respectively; After maturation, add up the output performance of test cross combination, rice blast and the false smut incidence of disease respectively, eliminate corresponding two-line sterile line; Now, the two-line sterile line of choosing is stayed to finalize the design almost.
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Cited By (7)
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| CN105918110A (en) * | 2016-05-10 | 2016-09-07 | 江苏农林职业技术学院 | Cultivation method for aromatic high-quality rice sterile line |
| CN106332776A (en) * | 2016-11-16 | 2017-01-18 | 重庆市农业科学院 | Method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice |
| CN106665365A (en) * | 2017-02-24 | 2017-05-17 | 张瑞明 | Novel thermal-resistant japonica rice breeding method |
| CN108967187A (en) * | 2018-09-29 | 2018-12-11 | 江西省超级水稻研究发展中心 | A kind of rice chalky grain rate high temperature insensitiveness germplasm selection |
| CN111436365A (en) * | 2020-03-25 | 2020-07-24 | 上海天谷生物科技股份有限公司 | Water-saving drought-resistant rice two-line sterile line cultivation method |
| CN113229140A (en) * | 2021-06-04 | 2021-08-10 | 广西星霞农业科技有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant two-line sterile line of rice |
| CN115176699A (en) * | 2022-08-01 | 2022-10-14 | 湖南仕叶生态农业科技发展有限公司 | Rice cultivation technology |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105918110A (en) * | 2016-05-10 | 2016-09-07 | 江苏农林职业技术学院 | Cultivation method for aromatic high-quality rice sterile line |
| CN106332776A (en) * | 2016-11-16 | 2017-01-18 | 重庆市农业科学院 | Method for breeding high-quality disease-resistant wild-abortion cytoplasm male sterile rice |
| CN106665365A (en) * | 2017-02-24 | 2017-05-17 | 张瑞明 | Novel thermal-resistant japonica rice breeding method |
| CN108967187A (en) * | 2018-09-29 | 2018-12-11 | 江西省超级水稻研究发展中心 | A kind of rice chalky grain rate high temperature insensitiveness germplasm selection |
| CN111436365A (en) * | 2020-03-25 | 2020-07-24 | 上海天谷生物科技股份有限公司 | Water-saving drought-resistant rice two-line sterile line cultivation method |
| CN111436365B (en) * | 2020-03-25 | 2023-08-18 | 上海天谷生物科技股份有限公司 | Cultivation method of water-saving drought-resistant rice two-line sterile line |
| CN113229140A (en) * | 2021-06-04 | 2021-08-10 | 广西星霞农业科技有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant two-line sterile line of rice |
| CN115176699A (en) * | 2022-08-01 | 2022-10-14 | 湖南仕叶生态农业科技发展有限公司 | Rice cultivation technology |
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