CN105116027B - Propylthiouracil (PTU) concentration analysis device and method based on surface acoustic wave series resonator biology tongue - Google Patents
Propylthiouracil (PTU) concentration analysis device and method based on surface acoustic wave series resonator biology tongue Download PDFInfo
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- CN105116027B CN105116027B CN201510067249.9A CN201510067249A CN105116027B CN 105116027 B CN105116027 B CN 105116027B CN 201510067249 A CN201510067249 A CN 201510067249A CN 105116027 B CN105116027 B CN 105116027B
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Abstract
The present invention relates to a kind of propylthiouracil (PTU) concentration analysis device and method based on surface acoustic wave series resonator biology tongue.Solve the problems, such as existing high using concentration analyzer cost.Device includes collecting work platform, collecting unit, converting unit and processing unit, collecting work platform includes pedestal, disk is provided with pedestal, multiple liquid storage cylinders are provided with disk, the side lower part of disk one is provided with drip chamber, drip chamber bottom is provided with liquid droping head, standing groove is provided with the lower section pedestal of liquid droping head, collecting unit includes cell battery lead plate and SAW resonator, cell battery lead plate is located in standing groove, SAW resonator is connected with converting unit, and converting unit is connected with processing unit.It is an advantage of the invention that a variety of solution concentrations can be detected by simple in construction, low manufacture cost automatically, it is easy to operate, while reduce operating personnel's workload, save the time.Detection process is convenient and swift, and precision is high.
Description
Technical Field
The invention relates to a solution concentration detection technology, in particular to a propylthiouracil concentration analysis device based on a surface acoustic wave series resonator biological tongue and a propylthiouracil concentration analysis method based on the surface acoustic wave series resonator biological tongue.
Background
The concentration of the solution is often required to be detected in a laboratory, and when the concentration of the solution is detected, a special concentration analyzer is usually used for detection, but the price of the concentration analyzer is high, and less concentration analyzers require ten thousand yuan, so that the experiment cost is greatly increased. Therefore, it is necessary to design a concentration detection device with simple structure, fast operation and low cost, and a detection method thereof.
Disclosure of Invention
The invention mainly solves the problem of high cost of a concentration analyzer in the prior art, and provides the propylthiouracil concentration analysis device based on the surface acoustic wave series resonator biological tongue, which has the advantages of simple structure, quickness in operation and low cost.
The invention also provides a method for analyzing the concentration of propylthiouracil based on the surface acoustic wave series resonator biological tongue, which is rapid to operate.
The technical problem of the invention is mainly solved by the following technical scheme: the utility model provides a propylthiouracil concentration analysis device based on biological tongue of surface acoustic wave series resonator, includes acquisition work platform, acquisition unit, conversion unit and processing unit, acquisition work platform includes the pedestal, and the rotation is provided with the disc on the pedestal, is provided with a plurality of stock solution chambeies around the centre of a circle on the disc, is provided with the drip chamber in disc one side lower part, drip chamber upper portion and disc bottom contact are provided with the liquid outlet bottom the stock solution chamber, are provided with first solenoid valve on the liquid outlet, correspond the liquid outlet position at drip chamber top and be provided with the inlet, be provided with the drip head on the drip chamber bottom, be provided with wiper mechanism and drainage mechanism in the drip chamber, be provided with the standing groove on the below pedestal of drip head, the acquisition unit is including the cell electrode board and the surface acoustic wave resonator that are connected, and the cell electrode board is located the standing groove, the resonator is connected with the conversion unit, and the conversion unit is connected with the processing unit. The invention discloses a cell electrode plate detection device, which comprises a cell electrode plate detection device, a surface acoustic wave resonator, a conversion unit, a processing unit and a sound wave signal processing unit. And after detecting the solutions with different concentrations, obtaining output signal-to-noise ratios respectively corresponding to the N digital signals, and then obtaining a solution concentration prediction model according to the fitted straight line. The disc can be placed with solutions with various concentrations, and the disc rotates at intervals according to the setting and adds the solution in one liquid storage cavity into the dropping liquid chamber. The drip chamber drips the solution onto the cell electrode plate, and after the detection of one concentration solution, the residual solution can be drained and the drip chamber can be cleaned. The invention can automatically analyze the concentration of the solution with different concentrations, has simple and convenient operation, does not need operators to always wait aside and manually replace the detection solution, reduces the workload of the operators and saves the time. The drip chamber has a cleaning function, when a solution with one concentration is discharged, the cleaning mechanism can be filled with water for cleaning, and filled with gas for drying, so that a solution with another concentration cannot be mixed with the residual liquid before entering the drip chamber, the concentration of the solution cannot be changed, and the accuracy of final analysis data is ensured.
As a preferable scheme, the cleaning mechanism comprises a water inlet and an air inlet which are communicated with the interior of the drip chamber, the water inlet is connected with the water tank, the air inlet is connected with the air pump, a water inlet electromagnetic valve and an air inlet electromagnetic valve are respectively arranged on the water inlet and the air inlet, the drainage mechanism comprises a drainage port arranged at the bottom of the drip chamber, and a drainage electromagnetic valve is arranged on the drainage port. The cleaning mechanism cleans the drip chamber. Open the outlet during washing, let in the clear water through the water inlet and carry out the water washing, let in the air through the air inlet after the water washing and dry in the dropping liquid chamber, this solution that makes later to add can not mix with the residual liquid before for solution concentration can not change, guarantees the accuracy nature of analysis result.
Preferably, the dropping head is communicated with the dropping liquid chamber through a micro pump. The solution in the dropping chamber is dropped on the cell electrode plate according to the set amount and time by a micro pump.
As a preferred scheme, be provided with the rabbling mechanism that adds oxygen in the drip chamber, the rabbling mechanism that adds oxygen includes the pivot, is provided with the pipeline in the pivot, the pivot is linked together with water inlet and air inlet, is connected with the stirred tube in the pivot, and the intermediate position of stirred tube is provided with the pivot seat, and the stirred tube passes through the pivot seat according to in the pivot, constitutes T type structure, and the stirred tube is hollow sealed tube, and the stirred tube is linked together with the pivot, serves at the end of stirred tube to be provided with a plurality of first gas pockets, is provided with a plurality of second gas pockets on the one side that the other end of stirred tube carried on the back with the first gas pocket. Add oxygen rabbling mechanism with the air let in the indoor solution of dropping liquid, increase the oxygen content of solution, let in the air simultaneously and can drive the stirring pipe and rotate, can stir solution for the better mixture of solution and air increases the oxygen content, and the back on the cell plate electrode is dripped to the solution like this, can make the cell on the cell plate electrode survive better, and the life-span of surviving is longer, has guaranteed the accuracy of test data.
As a preferred scheme, a limiting block is arranged on the top of the drip chamber, the surface of the limiting block is an arc-shaped surface, the surface of the limiting block is close to the side surface of the disc, a ball is arranged on the side surface of the disc corresponding to each liquid storage cavity, and a clamping groove is correspondingly arranged on the surface of the limiting block. The ball imbeds in the disc side, and the ball exposes the part and contacts with the stopper surface, and the disc rotates more smoothly under the ball effect like this, and when the disc was rotatory to target in place, the ball just in time imbeds in the draw-in groove, formed the location for the liquid outlet can better aim at with the inlet.
Preferably, a working electrode, a counter electrode and two reference electrodes are arranged on the cell electrode plate, the front end of the working electrode is circular, the circular part is made of foam copper, a gold-plated layer is further plated on the foam copper, cells are attached to the front end of the working electrode, the front ends of the counter electrode and the reference electrodes are arranged around the front end of the working electrode, and the outer parts of the front ends of the working electrode, the counter electrode and the reference electrodes are coated with a paint coating. The electrode is made of foam copper, the foam copper is of a three-dimensional reticular pore structure which is uniformly distributed, the electrode has a multi-layer stable reticular structure and is tightly connected, and the reticular structure is not easy to deform or collapse. This allows the cells to enter the interior of the copper foam structure, allowing the cells to better adhere to the electrodes. Compared with the copper foam, the gold-plated layer has lower toxicity, and the contact with cells leads to better cell activity and better detection sensitivity.
As a preferable scheme, a rotating motor is vertically arranged on the base, the disc is arranged on a rotating shaft of the rotating motor, a shaft sleeve is arranged outside the rotating shaft of the rotating motor, and the drip chamber is fixed on the shaft sleeve.
A propylthiouracil concentration analysis method based on a surface acoustic wave series resonator biological tongue comprises the following steps:
the method comprises the following steps: putting the propylthiouracil solution into a liquid storage cavity opposite to the drip chamber in the disc, and then injecting the solution in the liquid storage cavity into the drip chamber; the liquid storage cavity is opposite to the drip chamber, namely, the liquid outlet of the liquid storage cavity is aligned with the liquid inlet of the drip chamber.
Step two: dropping liquid on the cell electrode plate by a micro pump, setting the liquid dropping amount of each time to be 0.05ml, setting the liquid dropping interval time to be 3.2 seconds, setting the liquid dropping times to be 120 times, and after the liquid dropping is finished, discharging residual solution in the liquid dropping chamber and cleaning and drying the liquid dropping chamber; and opening the water discharge electromagnetic valve to discharge the residual solution in the dropping liquid chamber, and closing the water discharge electromagnetic valve after discharging. Then open the solenoid valve of intaking and let in clear water, wash in the dropping liquid room, open the drainage solenoid valve again after the washing and carry out the drainage. Closing the water inlet electromagnetic valve, opening the air inlet electromagnetic valve, introducing gas to blow dry the dropping liquid chamber, and finally closing the water drainage electromagnetic valve.
Step three: the acoustic surface wave resonator collects signals on a cell electrode plate and outputs the signals in a frequency form, the conversion unit converts the frequency signals to obtain a frequency curve, then the frequency curve is transmitted to the processing unit, and the processing unit samples the frequency curve to obtain an input value S (t); substituting the input value S (t) into a second-order linear system stochastic resonance model, which is:
and making the second order linear coefficient stochastic resonance model resonate; where x (t) is the vibration particle displacement, omega is the angular frequency, r and omega are the set attenuation coefficient and the frequency of the linear vibration particle respectively, c is the set signal modulation coefficient, b is the set quadratic noise xi 2 Coefficient of (t)Xi (t) is three-branched noise, xi (t) is formed by { -a,0, a }, a is more than 0, the disproportionation process of the noise follows Poisson distribution, and the probability distribution of the noise is p s (a)＝p s (-a)＝q，p s (0) =1-2q, wherein 0 < q < 0.5; noise mean and correlation follow<ξ(t)>＝0，<ξ(t)ξ(t+τ)>＝2qa 2 e -λτ (ii) a λ is the correlation rate, and the straightness of the three-branch noise ξ (t) isObtaining the output signal-to-noise ratio of the solution
Step four: substituting the output signal-to-noise ratio into a thiouracil solution concentration prediction model:
and (4) calculating the concentration of the propylthiouracil solution.
As a preferable scheme, the propythroxypyrimidine solution concentration prediction model is calculated by the following steps:
step a: respectively preparing propylthiouracil solutions with various concentrations, respectively placing the propylthiouracil solutions in the liquid storage cavities of the disks, and simultaneously inputting the corresponding concentration values k of the propylthiouracil solutions into the processor 1 ，k 2 ，...，k N (ii) a And N is the amount of the detection solution.
Step b: injecting the solution in the liquid storage cavity opposite to the drip chamber in the disc into the drip chamber, and obtaining the output signal-to-noise ratio of the solution according to the second step and the third step;
step c: rotating the disc to enable the next liquid storage cavity to be opposite to the drip chamber, repeating the step b until all the solutions on the disc are detected, and finally obtaining the output signal-to-noise ratio SNR corresponding to the solutions by the processor 1 ，SNR 2 ，...，SNR N Then according to the corresponding solution concentration k 1 ，k 2 ，...，k N Using (k) 1 ，SNR 1 )，(k 2 ，SNR 2 )，...，(k N ，SNR N ) Fitting into a straight line, and obtaining a thiouracil propylthiouracil solution concentration prediction model according to the fitted straight line:
therefore, the invention has the advantages that: simple structure, the cost of manufacture is low, can detect multiple solution concentration automatically, and convenient operation is swift, has reduced operating personnel work load simultaneously, the time of having practiced thrift. The detection process is convenient and fast, and the precision is high.
Drawings
FIG. 1 is a block diagram of one construction of the present invention;
FIG. 2 is a schematic structural view of an acquisition workstation according to the present invention;
FIG. 3 is a schematic structural view of an oxygenating stirring mechanism in the present invention;
FIG. 4 is a schematic diagram of a cell electrode plate according to the present invention.
1-acquisition workbench 2-acquisition unit 3-conversion unit 4-processing unit 5-surface acoustic wave resonator 6-cell electrode plate 7-base 8-disc 9-liquid storage cavity 10-drip chamber 11-placement groove 12-liquid outlet 13-liquid outlet electromagnetic valve 14-liquid inlet 15-drip head 16-air inlet 17-air inlet electromagnetic valve 18-water inlet 19-water inlet electromagnetic valve 20-water outlet 21-water discharge electromagnetic valve 22-rotating motor 23-shaft sleeve 24-micro pump 25-rotating shaft 26-stirring pipe 27-rotating shaft seat 28-first air hole 29-second air hole 30-ball 31-working electrode 32-counter electrode 33-reference electrode 34-paint coating 35-card slot
Detailed Description
The technical scheme of the invention is further specifically described by the following embodiments and the accompanying drawings.
Example (b):
the embodiment provides a propylthiouracil concentration analysis device based on a surface acoustic wave series resonator biological tongue, which comprises an acquisition workbench 1, an acquisition unit 2, a conversion unit 3 and a processing unit 4.
As shown in fig. 2, the collecting workbench comprises a base 7, a rotating motor 22 is vertically installed on the base, a disc 8 is arranged on a rotating shaft of the rotating motor, and a shaft sleeve 23 is arranged outside the rotating shaft of the rotating motor. A plurality of liquid storage cavities 9 for placing solution are uniformly arranged on the disc around the circle center, a drip chamber 10 is arranged at the lower part of one side of the disc, the drip chamber is fixedly connected on the shaft sleeve, and the upper part of the drip chamber is contacted with the bottom of the disc. Be provided with the stopper on drip chamber 10 top, the stopper surface is the arcwall face, and the stopper surface is pressed close to with the disc side mutually, corresponds every stock solution chamber position on the disc side and is provided with ball 30, specifically be equipped with the ball hole on stock solution chamber lateral wall, and ball hole opening is less than the inner chamber, and the ball card is downthehole at the ball, is equipped with the spring between ball and ball hole bottom, corresponds at the stopper surface and is provided with draw-in groove 35.
A liquid outlet 12 is arranged at the bottom of the liquid storage cavity, a liquid outlet electromagnetic valve 13 is arranged on the liquid outlet, a liquid inlet 14 is arranged at the upper part of the liquid storage chamber corresponding to the position of the liquid outlet, and the diameter of the liquid inlet is larger than that of the liquid outlet. The bottom of the drip chamber is provided with a drip head 15, and the drip head is provided with a micro pump 24 which is communicated with the interior of the drip chamber through the micro pump.
A cleaning mechanism and a drainage mechanism are arranged in the drip chamber, the cleaning mechanism comprises a water inlet 18 and a gas inlet 16, and the gas inlet and the water inlet are converged into a pipeline and then communicated with the interior of the drip chamber. The water inlet is provided with a water inlet electromagnetic valve 19, the water inlet is connected with the water tank through a pipeline, and the pipeline is provided with a water pump. An air inlet electromagnetic valve 17 is arranged on the air inlet, and the air inlet is connected with the air pump. The drain mechanism includes a drain port 20 provided at the bottom of the drip chamber, and a drain solenoid valve 21 is provided at the drain port.
Still be provided with the rabbling mechanism that adds oxygen in the dropping liquid chamber, as shown in fig. 3, the rabbling mechanism that adds oxygen includes pivot 25, is provided with the pipeline in the pivot, and the pipeline is linked together with water inlet and air inlet in the pivot, is connected with stirring pipe 26 in the pivot, and the intermediate position of stirring pipe is provided with pivot seat 27, and the stirring pipe is installed in the pivot through the pivot seat, constitutes T type structure, and the stirring pipe is hollow seal tube, and the stirring pipe is linked together with the pivot. A plurality of first air holes 28 are arranged at one end of the stirring pipe, and a plurality of second air holes 29 are arranged at the other end of the stirring pipe on the side opposite to the first air holes.
A placing groove 11 is arranged on the base at the lower part of the liquid dropping head, and a water outlet is arranged at the bottom of the placing groove.
The acquisition unit comprises a cell electrode plate 6 and a surface acoustic wave resonator 5, and the cell electrode plate is connected to the surface acoustic wave resonator. During operation, the cell electrode plate is placed in the placing groove of the collecting workbench. As shown in fig. 1, the surface acoustic wave resonator is connected to the conversion unit, the conversion unit is connected to the processing unit, and the surface acoustic wave resonator is connected to a power supply. As shown in FIG. 4, the cell electrode plate is provided with a working electrode 31, a counter electrode 32 and two reference electrodes 33. The front end of the working electrode is circular, the circular portion is made of copper foam, and a gold plating layer is further plated on the copper foam, cells are attached to the front end of the working electrode, the front ends of the counter electrode and the reference electrode are arranged around the front end of the working electrode, and a paint coating 34 is coated on the outer portions of the front ends of the working electrode, the counter electrode and the reference electrode.
The propylthiouracil concentration analysis method based on the acoustic surface wave series resonator biological tongue comprises the following steps:
the method comprises the following steps: the propylthiouracil solution was placed in the reservoir chamber opposite the drip chamber in the disk and the solution in the reservoir chamber was then injected into the drip chamber.
Step two: dropping liquid on the cell electrode plate by a micro pump, wherein the amount of the dropping liquid is set to be 0.05ml per time, the interval time of the dropping liquid is set to be 3.2 seconds, and the dropping frequency is set to be 120 times. After the liquid dropping is finished, the water discharging electromagnetic valve is opened to discharge the residual solution in the liquid dropping chamber, and the water discharging electromagnetic valve is closed after the residual solution is discharged. Then the water inlet electromagnetic valve is opened to introduce clear water, the dropping liquid chamber is washed, and the water discharge electromagnetic valve is opened to discharge water after the dropping liquid chamber is washed. Closing the water inlet electromagnetic valve, opening the air inlet electromagnetic valve, introducing gas to blow dry the dropping liquid chamber, and finally closing the water drainage electromagnetic valve.
Step three: the acoustic surface wave resonator collects signals on a cell electrode plate and outputs the signals in a frequency form, the conversion unit converts the frequency signals to obtain a frequency curve, then the frequency curve is transmitted to the processing unit, and the processing unit samples the frequency curve to obtain an input value S (t); substituting the input value S (t) into a second-order linear system stochastic resonance model which is:
and making the second order linear coefficient stochastic resonance model resonate; where x (t) is the vibration particle displacement, omega is the angular frequency, r and omega are the set attenuation coefficient and the frequency of the linear vibration particle respectively, c is the set signal modulation coefficient, b is the set quadratic noise xi 2 Coefficient of (t), xi (t) is three-way noise, xi (t) is epsilon { -a,0, a }, a > 0, and the disproportionation process of the noise follows Poisson distribution with probability distribution p s (a)＝p s (-a)＝q，p s (0) =1-2q, wherein 0 < q < 0.5; noise mean and correlation follow<ξ(t)>＝0，<ξ(t)ξ(t+τ)>＝2qa 2 e -λτ (ii) a λ is the correlation rate, and the straightness of the three-branch noise ξ (t) isObtaining the output signal-to-noise ratio of the solution
Step four: substituting the output signal-to-noise ratio into a thiouracil solution concentration prediction model:
and calculating the concentration of the propylthiouracil solution.
Wherein the propylthiouracil solution concentration prediction model is calculated by the following steps:
step a: respectively preparing propylthiouracil solutions with various concentrations, respectively placing the propylthiouracil solutions in the liquid storage cavities of the disks, and simultaneously inputting the corresponding concentration values k of the propylthiouracil solutions into the processor 1 ，k 2 ，...，k N ；
Step b: injecting the solution in the liquid storage cavity opposite to the drip chamber in the disc into the drip chamber, and obtaining the output signal-to-noise ratio of the solution according to the second step and the third step;
step c: c, rotating the disc to enable the next liquid storage cavity to be opposite to the dripping liquid cavity, repeating the step b until all the solutions on the disc are detected, and finally obtaining the output signal-to-noise ratio SNR corresponding to the solutions by the processor 1 ，SNR 2 ，...，SNR N Then according to the corresponding solution concentration k 1 ，k 2 ，...，k N Using (k) 1 ，SNR 1 )，(k 2 ，SNR 2 )，...，(k N ，SNR N ) Fitting into a straight line, and obtaining a thiouracil propylthiouracil solution concentration prediction model according to the fitted straight line:
the specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications or additions may be made to the described embodiments or alternatives may be employed by those skilled in the art without departing from the spirit or ambit of the invention as defined in the appended claims.
Although the terms acquisition stage, acquisition unit, conversion unit, processing unit, etc. are used more herein, the possibility of using other terms is not excluded. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed as being without limitation to any additional limitations that may be imposed by the spirit of the present invention.
Claims (9)
1. A propylthiouracil concentration analysis device based on acoustic surface wave series resonator biological tongue is characterized in that: including gathering workstation (1), collection unit (2), conversion unit (3) and processing unit (4), gathering workstation includes base (7), and the rotation is provided with disc (8) on the base erect and be provided with rotating electrical machines (22) on base (7), and disc (8) set up in rotating electrical machines's pivot, are provided with a plurality of stock solution chambeies (9) around the centre of a circle on the disc, are provided with drip chamber (10) in disc one side lower part, drip chamber upper portion and disc bottom contact are provided with liquid outlet (12) bottom the stock solution chamber, are provided with out liquid solenoid valve (13) on the liquid outlet, correspond the liquid outlet position at the drip chamber top and are provided with inlet (14), be provided with drip chamber (15) on the drip chamber bottom, be provided with wiper mechanism and drainage mechanism in the drip chamber, be provided with standing groove (11) on the below base of drip chamber, the collection unit is including cell plate electrode (6) and sound surface wave resonator (5) that are connected, and the cell plate electrode is located the standing groove, the surface acoustic wave resonator is connected with the conversion unit.
2. The apparatus for analyzing the propylthiouracil concentration based on SAW-SER biological tongue as claimed in claim 1, wherein said cleaning mechanism comprises a water inlet (18) and an air inlet (16) communicating with the interior of the drip chamber, the water inlet is connected to the water tank, the air inlet is connected to the air pump, a water inlet solenoid valve (19) and an air inlet solenoid valve (17) are respectively disposed on the water inlet and the air inlet, said water draining mechanism comprises a water draining port (20) disposed at the bottom of the drip chamber, and a water draining solenoid valve (21) is disposed on the water draining port.
3. The device for analyzing the propylthiouracil concentration based on SAW-SER biological tongue as claimed in claim 1, wherein said dripper (15) is connected to the drip chamber (10) through a micropump (24).
4. The apparatus for analyzing propylthiouracil concentration based on SAW-SERS resonator biological tongue as claimed in claim 2, wherein an oxygenation stirring mechanism is disposed in the drip chamber (10), the oxygenation stirring mechanism comprises a rotating shaft (25), a pipeline is disposed in the rotating shaft, the rotating shaft is communicated with the water inlet (18) and the air inlet (16), a stirring pipe (26) is connected to the rotating shaft, a rotating shaft seat (27) is disposed in the middle of the stirring pipe, the stirring pipe is mounted on the rotating shaft through the rotating shaft seat to form a T-shaped structure, the stirring pipe is a hollow sealing pipe, the stirring pipe is communicated with the rotating shaft, a plurality of first air holes (28) are disposed at one end of the stirring pipe, and a plurality of second air holes (29) are disposed at the other end of the stirring pipe and on the side opposite to the first air holes.
5. The apparatus for analyzing propylthiouracil concentration based on SAW-SER biological tongue as claimed in claim 1, 2, 3 or 4, wherein the top of said drip chamber (10) is provided with a limiting block, the surface of the limiting block is arc-shaped, the surface of the limiting block is close to the side of the disc, the side of the disc is provided with a ball (30) corresponding to each reservoir, and the surface of the limiting block is provided with a slot (35).
6. The device for analyzing the propylthiouracil concentration based on SAW-SER biological tongue as claimed in claim 1, 2, 3 or 4, wherein said cellular electrode plate (6) is provided with a working electrode (31), a counter electrode (32) and two reference electrodes (33), the front end of said working electrode is circular, the circular portion is made of copper foam, and a gold plating layer is further plated on said copper foam, the cells are attached to the front end of the working electrode, the front ends of said counter electrode and reference electrodes are disposed around the front end of the working electrode, and the outer portions of the front ends of the working electrode, counter electrode and reference electrodes are coated with a paint coating (34).
7. The apparatus for analyzing propylthiouracil concentration based on SAW-SERS biological tongue as claimed in claim 1, 2, 3 or 4, wherein a shaft sleeve (23) is disposed outside the rotating shaft of the rotating motor, and the drip chamber is fixed on the shaft sleeve.
8. A propylthiouracil concentration analysis method based on surface acoustic wave series resonator biological tongue, using the device in any one of claims 1-7, characterized by comprising the following steps:
the method comprises the following steps: putting the propylthiouracil solution into a liquid storage cavity opposite to the drip chamber in the disc, and then injecting the solution in the liquid storage cavity into the drip chamber;
step two: dropping liquid on the cell electrode plate by a micro pump, setting the liquid dropping amount of each time to be 0.05ml, setting the liquid dropping interval time to be 3.2 seconds, setting the liquid dropping times to be 120 times, and after the liquid dropping is finished, discharging residual solution in the liquid dropping chamber and cleaning and drying the liquid dropping chamber;
step three: the acoustic surface wave resonator collects signals on a cell electrode plate and outputs the signals in a frequency form, the conversion unit converts the frequency signals to obtain a frequency curve, then the frequency curve is transmitted to the processing unit, and the processing unit samples the frequency curve to obtain an input value S (t); substituting the input value S (t) into a second-order linear system stochastic resonance model, which is:
and making the second order linear coefficient stochastic resonance model resonate; where x (t) is vibration particle displacement, omega is angular frequency, r and omega are respectively set attenuation coefficient and linear vibration particle frequency, c is set signal modulation coefficient, b is set quadratic noise xi 2 Coefficient of (t), xi (t) is three-way noise, xi (t) is epsilon { -a,0, a }, a > 0, and the disproportionation process of the noise follows Poisson distribution with probability distribution p s (a)＝p s (-a)＝q，p s (0) =1-2q, wherein 0<q&0.5; noise mean and correlation follow<ξ(t)>＝0，<ξ(t)ξ(t+τ)>＝2qa 2 e -λτ (ii) a λ is the correlation rate, and the straightness of the three-branch noise ξ (t) isObtaining the output signal-to-noise ratio of the solution
Step four: substituting the output signal-to-noise ratio value into a propylthiouracil solution concentration prediction model:
and calculating the concentration of the propylthiouracil solution.
9. The method for analyzing the concentration of propylthiouracil on the basis of the biological tongue of surface acoustic wave series resonator as claimed in claim 8, wherein said propylthiouracil solution concentration prediction model is calculated by the following steps:
step a: respectively preparing propylthiouracil solutions with various concentrations, respectively placing the propylthiouracil solutions in the liquid storage cavities of the disks, and simultaneously inputting the corresponding concentration values k of the propylthiouracil solutions into the processor 1 ,k 2 ,...,k N ；
Step b: injecting the solution in the liquid storage cavity opposite to the drip chamber in the disc into the drip chamber, and obtaining the output signal-to-noise ratio of the solution according to the second step and the third step;
step c: rotating the disc to enable the next liquid storage cavity to be opposite to the drip chamber, repeating the step b until all the solutions on the disc are detected, and finally obtaining the output signal-to-noise ratio SNR corresponding to the solutions by the processor 1 ,SNR 2 ,...,SNR N Then according to the corresponding solution concentration k 1 ,k 2 ,...,k N Using (k) 1 ,SNR 1 ),(k 2 ,SNR 2 ),...,(k N ,SNR N ) Fitting into a straight line, and obtaining a propyridyl oxysulfide solution concentration prediction model according to the fitted straight line:
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| CN204044164U (en) * | 2014-06-23 | 2014-12-24 | 珠海森龙生物科技有限公司 | Mini-clinical biochemical analyzer |
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| CN103117728A (en) * | 2013-03-07 | 2013-05-22 | 浙江工商大学 | Acoustic surface wave resonator |
| CN103163217A (en) * | 2013-03-07 | 2013-06-19 | 浙江工商大学 | Sound surface wave resonator series detection and detection method |
| CN103424525A (en) * | 2013-03-18 | 2013-12-04 | 浙江工商大学 | Milk quality detection equipment and method |
| CN103412004A (en) * | 2013-08-22 | 2013-11-27 | 浙江工商大学 | Method for detecting storage time of citrus sinensis |
| CN104089996A (en) * | 2014-01-17 | 2014-10-08 | 浙江工商大学 | Taste cell sensor using surface acoustic wave resonator and preparation method thereof |
| CN204044164U (en) * | 2014-06-23 | 2014-12-24 | 珠海森龙生物科技有限公司 | Mini-clinical biochemical analyzer |
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