CN108732135A - A kind of blood cell and analysis of protein device - Google Patents
A kind of blood cell and analysis of protein device Download PDFInfo
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- CN108732135A CN108732135A CN201810416554.8A CN201810416554A CN108732135A CN 108732135 A CN108732135 A CN 108732135A CN 201810416554 A CN201810416554 A CN 201810416554A CN 108732135 A CN108732135 A CN 108732135A
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- detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
Abstract
The present invention provides a kind of analytical equipment that blood cell is integrated with Protein Detection, including blood cell detection module and Protein Detection module, its Protein Detection module includes detection cup holder and reagent bottle frame, the detection cup holder is for carrying detection cup, the detection cup freely can be picked and placeed or be replaced from detection cup holder according to demand, and analytical equipment of the invention is easy to operate, as a result accurately, pollution rate is small, and production cost and use cost are low.
Description
Technical field
The present invention relates to medical instruments fields, more particularly to a kind of blood cell and analysis of protein device.
Background technology
Blood routine examination is most basic one in human body " three important inspections ".Blood is by liquid and tangible cell two
Most of composition, and blood routine examination be blood cellular portions.Blood cell analysis device includes three classification haemocytes point
Analyzer and five classification cellanalyzers.Three classification cellanalyzers are to carry out blood cell point using Kurt impedance principle
Analysis, is that leucocyte is divided into cellule group (lymphocyte), cell,intermediate mass (acidophil, basophilic by certain dilution
And monokaryon) and maxicell group (neutrophil leucocyte), five classify is divided into neutrophil leucocyte, lymphocyte, acidophil granules leucocyte
Cell, basophilic granulocyte, monocyte.
In recent years, the development based on inflammation detection technique, blood routine are analyzed in conjunction with a part of Protein Detection, can effectively be referred to
Clinical diagnosis is led, individual equipment is used to the detection of specific protein in whole blood sample at present, carries out the craft of a series of complex
Operation could be completed once to test, and its testing result is whole blood albumen concentration, if to be converted into clinical required blood
Clear concentration then needs whole blood HCT (hematocrit values：That is volume ratio of the red blood cell in blood sample) this parameter, and it is special
The analyzer for measuring specific protein is the HCT values that can not measure each whole blood sample, can only provide a fixed HCT value
(about 40%), calculates albumen concentration in serum, there are the errors in methodology, can not eliminate according to it.Therefore, it develops
Detection can be synchronized to blood cell analysis and specific protein, testing process is simple, the instrument of accurate result is obtained, for blood
For the clinical detection assays of liquid cell detection and specific protein, has highly important meaning.
Invention content
In view of the foregoing deficiencies of prior art, the purpose of the present invention is to provide a kind of detection simplicity, results accurately,
Solution at low cost.
A kind of blood cell of present invention offer and analysis of protein device, including blood cell detection module and Protein Detection mould
Block, wherein the Protein Detection module includes detection cup holder and reagent bottle frame, and the detection cup holder is for carrying detection cup, institute
Stating detection cup freely can pick and place or replace from detection cup holder.
Blood cell provided by the invention and Protein Detection integrated analysis device, wherein the reagent bottle frame is tried for carrying
Agent bottle,
Blood cell provided by the invention and Protein Detection integrated analysis device, further include a sampling component, described to adopt
Sample component includes sampling needle, horizontal movement unit and vertical and straight movement unit.
Blood cell provided by the invention and Protein Detection integrated analysis device, wherein the detection cup frame and reagent bottle frame
It is arranged side by side, is located on the horizontal movement path of sampling needle.
It is provided on blood cell and analysis of protein device provided by the invention, blood cell and analysis of protein device fuselage
One cavity corresponding with Protein Detection block size, to accommodate Protein Detection module；The cavity is preferably arranged on machine
Body lower part.
Blood cell and analysis of protein device provided by the invention, the Protein Detection module can be fixed on inside cavity
Or free movement is to inside cavity or outside, while movement, Protein Detection mould detection cup holder in the block and reagent bottle frame are also same
Shi Yundong, the Protein Detection module and the connection type of blood cell and analysis of protein device be straight-line guidance structure connect or
Rotate-stationary mode mechanism connects；The straight-line guidance structure is sliding rail, the linear guide, is still more preferably the linear guide, institute
It is preferably arc guide rail to state rotate-stationary mode structure.
Blood cell and analysis of protein device provided by the invention further comprises an optical system, the optics
System includes light source, optical telescopic sleeve, scattering light sensation optical element and transmitted light photosensitive element, and the light source is located at the one of detection cup holder
Side, optical telescopic sleeve are wrapped in light source surface, and scattering light sensation optical element and transmitted light photosensitive element are respectively positioned on opposite with light source position
The detection cup holder other side, light source passes through from detection cup, and light intensity signal is acquired via photosensitive element.
Protein Detection module provided by the invention further comprises a thermostat module, the thermostatically-controlled equipment packet
Containing temperature sensor, heating element and temperature switch, the heating element is located at detection cup holder bottom, and outside Protein Detection module
Shell phase connects, and the heating element for being heated to sample in detection cup, according to temperature sensor monitors examine by temperature switch
Determination opens or closes heating device after surveying cup temperature.
Protein Detection module provided by the invention, further comprises a liquid channel system, which includes dilution
Liquid reagent barrel, syringe unit and discharging of waste liquid unit, syringe are connected with sampling needle, and moving back and forth control by syringe adopts
Sample is directed to the absorption and discharge of blood sample, after quantitative sample is emitted into detection cup by sampling needle, moves directly to blood inspection
It surveys and carries out next step detection in module, liquid channel system does not include any specific detection cup detergent line and device.
Blood cell and analysis of protein device provided by the invention, wherein the detection cup holder and reagent bottle frame can be held
The number for carrying detection cup and reagent bottle can be one or more, and due to the limitation of equipment volume, preferably 1-5 is a, detection cup
Frame number is more preferably 1, and reagent bottle frame number is more preferably 1, and reagent bottle can freely pick and place and more from reagent bottle frame
It changes.
Blood cell and analysis of protein device provided by the invention, it is mixed that Protein Detection module further comprises a liquid
Even device, the liquid blending device are located at detection cup holder side, and the side is the right opposite of relative temperature sensor,
Including motor and magnet, magnet are connected on motor rotation axis, motor band moving magnet rotates, and further driving is located in detection cup
Stirrer rotate mixing liquid.
Blood cell and analysis of protein device provided by the invention can be used for detecting the protein content in sample, with prediction
Human health status.
The wherein described albumen is selected from CRP, human serum amyloid A serum amyloid A protein (SAA), PCT
Index in (Procalcitonin, procalcitonin) glycosylated hemoglobin, cardiac muscle five, index and inflammation in kidney.
The present invention further provides blood cell and analysis of protein device, the Protein Detection module can be with free movement
To inside cavity or outside, or it is fixed on inside cavity, while driving Protein Detection mould detection cup holder in the block and reagent bottle frame
Movement.
The present invention further provides blood cell and analysis of protein device, the detection cup holder or reagent cup holder and blood
The connection type of cell analysis apparatus is that straight-line guidance structure connects or rotate-stationary mode mechanism connects, the straight-line guidance structure
Selected from sliding rail, the linear guide, preferably the linear guide, the rotate-stationary mode structure is selected from arc guide rail.
Blood cell and analysis of protein device provided by the invention, wherein the blood cell detection module includes fluid path system
System, reaction tank, sampling component and signal detection component.
The wherein described liquid channel system includes the diluent reagent bucket, valve, syringe, sampling needle, swab, vacuum chamber, gives up
Liquid pump and waste liquid barrel, above-mentioned component are linked in sequence by pipeline.
The reaction tank includes WBC impedances pond, RBC impedances pond, HGB detection modules, and wherein HGB detection modules are surrounded on
WBC impedances pond side, WBC impedances pond and RBC impedances pond are arranged side by side.
The sampling component and Protein Detection module samples component share, including horizontal movement component, vertical and straight movement component,
Sampling needle；Wherein horizontal movement component is loaded on apparatus frame；It is another to have vertical and straight movement component on horizontal movement component, it can be with
Integral level moves forward and backward, and sampling needle is loaded on vertical and straight movement component.
The signal detection component is the ponds WBC impedance component, and it includes two electrodes, are located inside and outside impedance pond, in
Between be connected to by aperture.
Blood cell and analysis of protein device provided by the invention comprising following detecting step：
1, sampling needle draw blood sample；
2, sampling needle level carries blood sample and moves to reach above detection cup, and blood sample is quantitatively discharged；
3, sampling needle leaves detection cup, is moved respectively to the ponds WBC, the ponds RBC top, declines discharge and draw corresponding blood sample
Sheet, reagent, mixed liquor complete blood routine and divide sample；
4, sampling needle is moved to above the reagent bottle of Protein Detection module, after declining the reagent drawn used in Protein Detection,
It is moved to above detection cup, declines discharge reagent；
5, after starting detection, each reaction tank starts simultaneously at reaction, and after the completion of detection, instrument provides blood routine and albumen simultaneously
Testing result.
In technical solutions according to the invention, detection cup is for loading blood sample or the blood sample containing reagent
This, according to detection demand, the quantity of detection cup can be one or more；Further, in certain embodiments, detection cup
It is disposable, without cleaning and safeguards, can be replaced according to different samples.
In embodiments of the invention, reagent bottle frame is for carrying reagent bottle, further, in certain embodiments
In, the number of reagent bottle can be one, two or more.
Further, in certain embodiments, one or more stirrers can be placed in sample cup, further
, the stirrer is magnetic stir bar.
In embodiments of the invention, Protein Detection mould it is in the block heating and temperature control device be used for ensure sample cup and its
Mixed liquor is included to be reacted and detected in steady temperature.
It in embodiments of the invention, can people when Protein Detection mould detection cup holder in the block is fixed on inside cavity
Hand is stretched into instrument the replacement for being detected cup by work.
The present invention does not limit the concrete structure and function of blood cell detection module, and one on the basis of present inventive concept
Shear deformation is all in the scope of the present invention.
Beneficial effects of the present invention are：
1. primary blood sampling can complete blood cell analysis and analysis of protein, easy to operate；
2. analysis of protein result is accurate, the unknown Protein Detection error brought of HCT values is eliminated；
3. detection cup, reagent bottle are removable and replace, it is suitable for different Protein Detection projects, measure the item extension spirit
It is living, have a wide range of application；
4. instrument internal is not necessarily to configure the cleaning system of Protein Detection module, space is greatly saved, reduces instrument life
Produce cost；
5. instrument internal need not clean test cup every time, reagent need not be also filled in corresponding pipeline, therefore, no
Extra consumption reagent is needed, the cost of user's reagent consumption is reduced；
6. independent detection cup, it is possible to prevente effectively from carrying the result mistake come in contaminated zone between sample.
Description of the drawings
Fig. 1 is the dimensional structure diagram that blood cell is integrated with Protein Detection in the embodiment of the present invention；
Fig. 2 is the structural schematic diagram of the detection components in Protein Detection device in the present invention；
Fig. 3 is the cross-sectional view of Protein Detection module in the present invention；
Fig. 4 is the structural schematic diagram of Protein Detection mould thermostat module in the block and apparatus for combining liquids in the present invention；
Fig. 5 is the internal structure schematic diagram that blood cell is integrated with Protein Detection in reality of the present invention；
Fig. 6 is the structural schematic diagram of liquid channel system in the present invention；
Reference numeral：
1, blood cell detection module；011, RBC impedances pond；012, WBC impedances pond；013, HGB detection modules；2, albumen
Detection module；021, reagent bottle frame；022, cup holder is detected；0221, optical telescopic sleeve；0222, light source；0223, detection cup deck；
0224, transmitted light photosensitive element；0225, light sensation optical element is scattered；023, thermostat module；0231, temperature sensor；024, albumen
Detection device pedestal；3, synchronous belt；4, the second motor is driven；5, sampling needle；6, horizontal movement unit；7, vertical and straight movement unit；
8, the linear guide；9, driving pulley；10, belt support frame；11, driven pulley；12, first motor mounting bracket；13, the first electricity
Machine；14, magnet；15, magnet fixed seat；16, diluent reagent bucket；17, the first syringe；18, the of the first three-way magnetic valve
Two passways；19, the first passage mouth of the first three-way magnetic valve；20, the third channel mouth of the first three-way magnetic valve；21, second
The second channel mouth of three-way magnetic valve；22, the third channel mouth of the second three-way magnetic valve；23, the first of the second three-way magnetic valve
Passway；24, the second syringe；25, the inlet of swab；26, the liquid outlet of swab；27, the first solenoid valve；28, the second electricity
Magnet valve；29, vacuum chamber；30, waste drains pump；31, waste liquid barrel.
Specific implementation mode
Embodiments of the present invention are illustrated by specific specific example below in conjunction with attached drawing, those skilled in the art can be by
Content disclosed by this specification understands other advantages and effect of the present invention.The present invention can also be by addition different specific
Embodiment is embodied or practiced, it should be understood that under the premise of without departing from essence of the present invention, according to scope of the invention institute
The equivalent change or modification that the structure, feature and principle stated is done, within the scope of the present invention.
It should be further understood that the combination connection relation between one or more equipment/device mentioned in the present invention is not
Repel there may also be other equipment/device before and after the unit equipment/device or specifically mention at these two equipment/
Other equipment/device can also be inserted between device, unless otherwise indicated, relativeness is altered or modified, in no essence
In the case of changing technology contents, when being also considered as the enforceable scope of the present invention.
Embodiment one
Referring to Fig.1, a kind of blood cell and protein analyzer, including installation shell, the mounting shell body is interior to be equipped with blood
Cell detection module 1, Protein Detection module 2 and sampling component, the Protein Detection device include the inspection for placing detection cup
Cup holder 022 and the reagent bottle frame 021 for placing reagent bottle are surveyed, cup holder is detected and reagent bottle frame is arranged side by side；The set of samples
Part is located at the top of blood test device and Protein Detection device, for drawing and being discharged sample liquid.Above-mentioned blood cell and albumen
In analyzer, 1~5 detection cup, preferably 1 can be placed by stating detection cup holder, and reagent bottle frame can place 1~5 reagent
Bottle, preferably 1, the detection cup freely can be picked and placeed or be replaced from detection cup holder, and reagent bottle can be from reagent bottle frame
It freely picks and places or replaces.
With reference to Fig. 2, the concrete structure of Protein Detection device is：Including Systems for optical inspection, Systems for optical inspection includes light
Source 0222, optical telescopic sleeve 0221, scattering light sensation optical element 0225, detection cup deck 0223 and transmitted light photosensitive element 0224, light
Source is located at the side of detection cup holder, and optical telescopic sleeve is socketed on light source surface, scatters light sensation optical element and transmitted light photosensitive element is equal
Positioned at the detection cup holder other side opposite with light source position；Transmitted light photosensitive element face light source is arranged, for acquiring through inspection
The transmitted ray of cup is surveyed, scattering light sensation optical element is set to the surrounding of transmitted light photosensitive element, for acquiring through detection cup
Scattering light.The power of light sensation optical element and transmitted light photosensitive element according to acquisition light signal is scattered, to detect albumen sample liquid
Concentration.As shown in Figure 3,4, Protein Detection device further includes thermostat module 023, thermostat module include temperature sensor 0231,
Heating element and temperature switch, the heating element are located at immediately below detection cup holder, are used for and the contact of detection cup bottom is to detection
Cup mixing liquid heats, and temperature sensor is close to detect wall of cup, and temperature switch is controlled according to temperature sensor testing result
Opening and closing.Thermostat module can make mixed liquor of the detection cup in it be reacted and be detected in steady temperature, ensure that detection was met
Accuracy.
In above-mentioned Protein Detection device, detection cup is used to accommodate the reaction reagent of Protein Detection, is located at Protein Detection device
Carrier and light source base on, workflow when Protein Detection is that sampling needle is quantitative, and blood sample is added into detection cup,
Reagent is added, while heating element works, temperature sensor monitors detect cup temperature, closing temperature when being higher than set temperature
Switch, temperature switch is opened when higher than set temperature, and temperature determination is returned after detecting cup temperature according to temperature sensor monitors
Open or close heating device, light source is passed through from detection cup when detection, light intensity signal is acquired via photosensitive element, into one
Step is converted into the concentration of target protein.
Further include the liquid blending device being arranged in detection cup holder side, liquid blending as shown in figure 3, in the present embodiment
Device includes first motor mounting bracket 12, first motor 13, magnet 14, magnetic stir bar and magnet fixed seat 15, and magnet is solid
On the output shaft of motor, magnetic stir bar drives magnetic for being arranged in detection cup, by the rotation of first motor for Dingan County
Power stirrer rotates.
As shown in figure 5, in the present embodiment, the blood test device includes reaction tank and signal detection component, reaction tank
Including WBC impedances pond 012, RBC impedances pond 011 and HGB detection modules 013, the HGB detection modules are surrounded on WBC impedances
Pond side, WBC impedances pond and RBC impedances pond are arranged side by side.
As shown in Fig. 1 and Fig. 5, in the present embodiment, the sampling component includes sampling needle 5, horizontal movement unit 6 and vertical
Moving cell 7, the horizontal movement unit are mounted on installation shell, and vertical and straight movement unit is installed on horizontal movement unit,
Sampling needle is installed on vertical and straight movement unit；By horizontal movement unit sampling needle is moved in the horizontal direction, by perpendicular
Straight moving cell enables sampling needle to be moved in vertical direction, and sampling component is drawn in the position of blood sample, Protein Detection device
Detection cup is divided at blood, detection reagent draws position in Protein Detection device, sampling component divides position and the set of samples of blood in the ponds WBC
Part divides the position of blood to be arranged successively along the motion track of the horizontal direction of sampling needle in the ponds RBC, when ensure that suction sample and detection
Accurately positioning, ensure that entire detection process safely and effectively carries out.
With reference to Fig. 6, the structure of liquid channel system is：Liquid channel system includes the dilution bucket 16 for passing sequentially through pipeline connection, note
Emitter and discharging of waste liquid module, syringe and waste liquid module can be controlled with sampling needle by pipeline connection by syringe
The waste liquid after sampling needle cleaning can be discharged by discharging of waste liquid module in absorption and discharge of the sampling needle to blood sample.Also wrap
The first three-way magnetic valve, the second three-way magnetic valve, the first solenoid valve 27 and second solenoid valve 28 are included, discharging of waste liquid module includes true
Empty room 29, waste drains pump 30 and waste liquid barrel 31, syringe include the first syringe 17 and the second syringe 24；First three-way magnetic valve
First passage mouth 19 be connected to diluent reagent bucket by pipeline, the second channel mouth 18 of the first three-way magnetic valve passes through pipeline
It is connected to the first syringe, the third channel mouth 20 of the first three-way magnetic valve is connected to by pipeline and the second syringe input port；
The first passage mouth 23 of second three-way magnetic valve is connected to by pipeline and the second syringe delivery outlet, and the of the second three-way magnetic valve
Two passways 21 are connected to after the first solenoid valve with the inlet of swab, and the third channel mouth 22 of the second three-way magnetic valve passes through
Pipeline is connected to sampling needle input port；The inlet 25 of swab is sequentially communicated vacuum chamber, waste drains pump and is given up after second solenoid valve
Liquid bucket.Fluid path workflow is when Protein Detection：Solenoid valve control syringe does advancing movement, discharges the quantitative liquid in sampling needle
Body enters Protein Detection cup, sampling needle continue movement complete blood testing module divide sample and dilution after, solenoid valve control injection
The dilution that device extracts in dilution bucket enters sampling needle cleaning part, is cleaned to sampling needle, and waste liquid is generated negative by waste drains pump
Pressure is extracted from pipeline and, and passes through vacuum chamber and waste drains pump successively, after being collected into waste liquid barrel, sampling needle is moved to examination
The top position of agent bottle declines and draws reagent, is moved to the top of Protein Detection cup, and solenoid valve control syringe does propulsion fortune
Dynamic, the liquid discharged in sampling needle enters in Protein Detection cup, starts to react, and after the completion of reaction, detection cup can be filled from analysis
It sets internal take out to clean, disposable detection cup can also be used, be not necessarily in whole device for protein detection system setting specially
Cleaning system, space is greatly saved, reduces instrument production cost, while examination need not be filled in corresponding pipeline
Agent therefore, there is no need to extra consumption reagent, reduce user's reagent consumption cost, independent detection cup, it is possible to prevente effectively from
The result mistake come in contaminated zone is carried between sample.
As shown in figure 3, in the present embodiment, the installation housing side is equipped with a switching door, in Protein Detection bottom of device
Rail plate 8 is installed, drive the second motor of driving 4 of rail plate, belt pulley (including driving pulley 9, synchronous belt 3 and
Driven pulley 11) and belt support frame 10 that belt pulley and rail plate link together, pass through motor and controls belt pulley
Movement, belt pulley movement so that drive rail plate rail plate movement, and then realize Protein Detection device pull out or
The action of installation shell is promoted, in the present embodiment, Protein Detection device is also securable in mounting shell body.Above-mentioned blood cell
Be equipped with the accommodating chamber for accommodating the Protein Detection device and in protein analyzer, in mounting shell body, the size of accommodating chamber and
Protein Detection device matches, and accommodating chamber is preferably provided at lower part in mounting shell body, and the corresponding switching door setting is being installed
Housing side is close to lower area.It, can be manually by hand when Protein Detection mould detection cup holder in the block is fixed on inside accommodating chamber
It stretches into instrument and is detected the replacement of cup.The present invention does not limit the concrete structure and function of blood cell detection module,
A shear deformation on the basis of present inventive concept is all in the scope of the present invention.
Blood cell and protein analyzer provided by the invention can be used for detecting the protein content in sample, to predict people
Body health status.The wherein described albumen be selected from CRP, human serum amyloid A serum amyloidAprotein (SAA),
PCT (Procalcitonin, procalcitonin) glycosylated hemoglobin, cardiac muscle five, kidney, inflammation.
In technical solutions according to the invention, detection cup is used to load blood sample or the blood sample containing reagent,
According to detection demand, the quantity of detection cup can be one or more；Further, in certain embodiments, detection cup is
Disposably, it is not necessarily to cleaning and safeguards, can be replaced according to different samples.
It is as follows to albumen and blood cell detecting step in the present embodiment：(by taking CRP as an example)
1. reagent bottle to be manually put into the CRP reagent bottle loading assemblies stretched out outside instrument, all reagent bottles are moved to
On the motion path of sampling needle unit, reagent is drawn for sampling needle unit；
2. detection cup to be manually put into the CRP loading assemblies stretched out outside instrument, detection cup is moved to sampling needle unit
Motion path on, draw blood sample to be measured and reagent for sampling needle unit；
3. reagent bottle and detection cup are below sampling needle unit motion path, position is adjacent；
4. by the bottom-up sampling needle list for being inserted in the original position immediately ahead of machine of the heparin tube equipped with blood sample, ensure
Blood sample liquid level or less is submerged in sampling needle unit bottom suction sample hole；Connected syringe starts drawing liquid after sampling needle unit, will determine
It measures in blood sample sucking sampling needle unit；
5. upper lift under the action of up and down motion unit of sampling needle unit, leaves heparin tube；
6. sampling needle unit carries blood sample horizontal rearward movement, reach right over CRP detection cups；
7. sampling needle unit drops to needle mouth and submerges in CRP detection cups below liquid level and quantitative blood sample is discharged；
8. sampling needle unit is lifted off CRP detection cups；
9. sampling needle unit is moved horizontally to WBC impedances pond, RBC impedances pond top successively, declines discharge and draw corresponding
Blood sample, reagent, mixed liquor complete blood routine and divide sample；
10. sampling needle unit horizontal is moved to above CRP reagent bottles position, declines and draw detection reagent used；
11. sampling needle unit is moved horizontally to after rising above CRP detection cups, decline discharge detection agents useful for same again；
12. module where detection cup starts to react, and starts detection；
13. after the completion of detection, instrument provides blood routine and CRP results simultaneously.Contents of the wherein CRP in serum can be by
True HCT parameters that blood routine measures are corrected；
Embodiment two
Linear motion guide rail described in embodiment one and the pivot structure that can be changed to rotation in horizontal plane, can also be fixed on instrument
It is motionless inside device, the replacement that instrument internal is detected cup and reagent bottle is manually stretched by human hand；
The integration mode of Protein Detection module of the present invention is not limited to above-mentioned realization method.
Claims (10)
1. a kind of blood cell and analysis of protein device, including blood cell detection module and Protein Detection module, the albumen
Detection module includes detection cup holder and reagent bottle frame, and the detection cup holder is for carrying detection cup, it is characterised in that：The detection
Cup freely can be picked and placeed or be replaced from detection cup holder.
2. blood cell according to claim 1 and analysis of protein device, it is characterised in that：It further include a set of samples
Part, the sampling component include sampling needle, horizontal movement unit and vertical and straight movement unit；The detection cup holder and reagent bottle frame are
It is arranged side by side, is more preferably located on the horizontal movement path of sampling needle.
3. blood cell according to claim 1 and analysis of protein device, it is characterised in that：The blood cell and albumen
There are one cavitys corresponding with Protein Detection block size for setting on analytical equipment fuselage, to accommodate Protein Detection module；Institute
It states cavity and is preferably arranged on underbelly.
4. blood cell according to claim 1 and analysis of protein device, it is characterised in that：The Protein Detection module can
To be fixed on inside cavity or free movement to inside cavity or outside, while movement, Protein Detection mould detection cup in the block
Frame also moves simultaneously with reagent bottle frame；The Protein Detection module and the connection type of blood cell and analysis of protein device are straight
Line guide frame connects or the connection of rotate-stationary mode mechanism；The straight-line guidance structure be sliding rail, the linear guide, further it is excellent
It is selected as the linear guide, the rotate-stationary mode mechanism is preferably arc guide rail；The detection cup holder can carry of detection cup
Number is one or more, and preferably 1-5 is a, more preferably 1.
5. blood cell according to claim 1 and analysis of protein device, it is characterised in that：The reagent bottle frame can
The number for carrying reagent bottle is one or more, and preferably 1-5, further preferably 1, the reagent bottle can be from reagent
It freely picks and places or replaces on bottle stand.
6. the blood cell according to any one of claim 1-5 and analysis of protein device, it is characterised in that：The egg
White detection module also includes an optical system, and the optical system includes light source, optical telescopic sleeve, scattering light sensation optical element and thoroughly
Light sensation optical element is penetrated, the light source is located at the side of detection cup holder, and optical telescopic sleeve is wrapped in light source surface, scatters light sensation optical element
It is respectively positioned on the detection cup holder other side opposite with light source position with transmitted light photosensitive element, light source is passed through from detection cup, via
Photosensitive element acquires light intensity signal；The preferably described Protein Detection module also includes a thermostat module, the constant temperature mould
Block includes temperature sensor, heating element and temperature switch, and the heating element is located at detection cup holder bottom, with Protein Detection mould
Block shell is connected, and the heating element for being heated to sample in detection cup, according to temperature sensor supervise by temperature switch
Determination opens or closes heating device after surveying detection cup temperature；The further preferably described Protein Detection module also includes one
Liquid channel system, the liquid channel system include diluent reagent bucket, syringe unit and discharging of waste liquid unit, syringe and sampling needle phase
Even, absorption and discharge of the control sampling needle to blood sample are moved back and forth by syringe, quantitative sample is emitted by sampling needle
After in detection cup, move directly to carry out next step detection in blood testing module, liquid channel system does not include any specific inspection
Survey cup detergent line and device.
7. the blood cell according to any one of claim 1-6 and analysis of protein device, it is characterised in that：The egg
White detection module also includes a liquid blending device, and the liquid blending device is located at detection cup holder side, the side
For the right opposite of relative temperature sensor, including motor, magnet and stirrer, magnet are connected on motor rotation axis, motor band
Moving magnet rotates, and further driving is located at the stirrer rotation mixing liquid in detection cup.
8. blood cell according to claim 1 and analysis of protein device, it is characterised in that：The Protein Detection module can be used for
Detect CRP, SAA, PCT, glycosylated hemoglobin, myocardium five, renal function.
9. the blood cell according to any one of claim 1-8 and analysis of protein device, it is characterised in that：The blood
Liquid cell detection module includes liquid channel system, reaction tank, sampling component and signal detection component；The preferably described liquid channel system packet
The diluent reagent bucket, valve, syringe, sampling needle, swab, vacuum chamber, waste drains pump and waste liquid barrel are included, above-mentioned component passes through pipe
Road is linked in sequence；The preferably described reaction tank includes WBC impedances pond, RBC impedances pond, HGB detection modules, wherein HGB detections
Module is surrounded on WBC impedances pond side, and WBC impedances pond and RBC impedances pond are arranged side by side；The preferably described sampling component and albumen
Detection module sampling component shares, and wherein for horizontal movement component loaded on apparatus frame, vertical and straight movement component is mounted in horizontal movement
On component, it can be moved forward and backward with integral level；Sampling needle is loaded on vertical and straight movement component.
10. the detection method of a kind of whole blood and albumen, which is characterized in that include the following steps：
1), sampling needle draw blood sample；
2), sampling needle level carries blood sample and moves to reach above detection cup, and blood sample is quantitatively discharged；
3), sampling needle leaves detection cup, is moved respectively to the ponds WBC, above the ponds RBC, decline discharge and draw corresponding blood sample,
Reagent, mixed liquor complete blood routine and divide sample；
4), sampling needle is moved to above the reagent bottle of Protein Detection module, mobile after declining the reagent drawn used in Protein Detection
Above to detection cup, decline discharge reagent；
5) after, starting detection, each reaction tank starts simultaneously at reaction, and after the completion of detection, instrument provides blood routine and albumen inspection simultaneously
It surveys.
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| CN109782001A (en) * | 2019-03-27 | 2019-05-21 | 苏州康和顺医疗技术有限公司 | A kind of household protein analyzer stirring system and its stirring means |
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| CN113466473A (en) * | 2020-03-31 | 2021-10-01 | 深圳市帝迈生物技术有限公司 | Sample analyzer and sample analysis method and system |
| CN113495087A (en) * | 2020-04-03 | 2021-10-12 | 深圳市帝迈生物技术有限公司 | POCT blood cell analyzer and detection method thereof |
| CN113495086A (en) * | 2020-04-03 | 2021-10-12 | 深圳市帝迈生物技术有限公司 | POCT blood cell analyzer and kit |
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| CN113917165A (en) * | 2020-07-10 | 2022-01-11 | 深圳市帝迈生物技术有限公司 | POCT sample analyzer and detection method thereof |
| CN113686760A (en) * | 2021-02-03 | 2021-11-23 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
| CN113686759A (en) * | 2021-02-03 | 2021-11-23 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
| CN113686759B (en) * | 2021-02-03 | 2023-11-24 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
| WO2023122973A1 (en) * | 2021-12-28 | 2023-07-06 | 深圳迈瑞动物医疗科技股份有限公司 | Multifunctional sample analysis device |
| CN114264804A (en) * | 2022-03-03 | 2022-04-01 | 深圳市帝迈生物技术有限公司 | Sample analyzer and detection method thereof |
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