CN1028472C - Breeding method for hybrid rice - Google Patents
Breeding method for hybrid rice Download PDFInfo
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- CN1028472C CN1028472C CN 92103511 CN92103511A CN1028472C CN 1028472 C CN1028472 C CN 1028472C CN 92103511 CN92103511 CN 92103511 CN 92103511 A CN92103511 A CN 92103511A CN 1028472 C CN1028472 C CN 1028472C
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- high resistance
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- hybridize
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Abstract
The present invention relates to a method for breeding Shanyoukang 65 of bacterial blight hybrid rice with high resistance. A DV85 derived resistant source is hybridized with Minghui 63 to breed restoring-line Nannongkang 65 which is hybridized with Zhenshan 97A to prepare hybrid species of Shanyoukang 65, which are used for production. Identification indicates that the shape and the growth characteristics of plants of the Nannongkang 65 are approximate to those of Shanyou 63, bacterial blight with high resistance is displayed in a blight region, and yield is increased by 20 to 50%. The present invention is suitable for the popularization and the application of hybrid rice regions in the south in China and has the advantages of saving agricultural cost and working hours and reducing pesticide pollution.
Description
The present invention relates to the selection of the new combination of hybrid rice of high resistance to hoja blanca.
More than 100,000,000 mu of Yangtze river basin medium rice area, hybridization long-grained nonglutinous rice proportion is bigger, and applied combination is single.Account for more than 90% as Jiangsu, Anhui two provinces " Shanyou 63 "." Shanyou 63 " also has as late rice or early rice in southern most of area, is the combination that present China hybrid rice is used the area maximum.This makes up high yield, adaptability is strong, has made outstanding contributions over past ten years on Rice Production.But because its easy infection bacterial leaf-blight, output is high and unstable, especially retransmits the district at province bacterial leaf-blight Chang Faqu such as Soviet Union, Anhui, Hubei Province, Henan, has been difficult to use on producing.Along with agricultural production develops to efficient, low consumption direction, the new combination of the hybrid rice of seed selection high resistance to hoja blanca has become key subjects in the breeding.Domestic and international many breeding work persons have made big quantity research for this reason, seed selection in recent years the recovery system of many bacterial blight-resistings as IR system (comprising bright extensive 63), special green grass or young crops etc.Yet how because of still not disease-resistant or middle bacterial blight-resistings such as the hybrid combination Shanyou 63 that utilizes this incomplete dominant gene of Xa-4 to prepare, Shanyougui 33 (see " rice in China kind and pedigree thereof " that Shanghai science tech publishing house publishes in March, 1991 P.197-209).
The objective of the invention is to solve in the present production the not a great problem of bacterial blight-resisting of hybrid rice improved seeds " Shanyou 63 ", a kind of breeding method of high resistance to hoja blanca is provided, breeding high resistance to white leaf-blast of hybrid rice newly makes up, make it both to keep the merit of " Shanyou 63 ", again can high resistance to hoja blanca, go up large-area applications for producing.
The breeding method of high resistance to white leaf-blast of hybrid rice new varieties comprises and selects disease-resistant material, the transformation of anti-source is fastened to the good recovery of comprehensive proterties, then in the preparing hybrid kind.
With coming (TN No. one in the platform
1) be maternal, the DV of bacterial blight-resisting
85Hybridize anti-source " TN in the middle of breeding for male parent
1/ DV
85".
With the anti-source " TN in centre
1/ DV
85" be maternal, bright extensive 63 hybridize for male parent, breed high resistance to hoja blanca and recover system's " Nan Nong resists 65 ".
With male sterile line Zhenshan 97a or imperial Mortopl A or Xieqingzao A is maternal, recovers system " Nan Nong anti-65 " and hybridizes for male parent, preparing hybrid kind " Shan excellent anti-65 " or " special excellent anti-65 " or " assist excellent resist 65 ".
Middle anti-source " TN
1/ DV
85" hybridize with bright extensive 63 after, and carry out backcrossing for 3-4 time, breed high resistance to hoja blanca and recover system's " Nan Nong resists 65 ".
Be that example is narrated advantage of the present invention and good effect with " Shan excellent anti-65 " below:
1, high resistance to hoja blanca.The hybrid rice that the present invention breeds newly makes up " Shan excellent anti-65 " and not only has " Shanyou 63 " good integral economic trait, and the performance high resistance to hoja blanca.In 1 day sick level of strong fungus strain " Zhejiang 73 " inoculation offspring is 0.7 grade, is 3.9 grades and contrast " Shanyou 63 " sick level.
2, high yield.Because " Shan excellent anti-65 " newly makes up bacterial blight-resisting, apparent fitness value is wide, and yielding ability is good.At bacterial leaf-blight Chang Faqu, " Shan excellent anti-65 " because high resistance to hoja blanca, anaphase blade is delicate and pretty, and production ratio " Shanyou 63 " (CK) improves 20-50%." Shan excellent anti-65 " because high resistance to hoja blanca has overcome " Shanyou 63 " unique defective on producing, its integral economic trait also is better than other hybrid rice combination.
Accompanying drawing is: high resistance to hoja blanca hybrid rice breeding line spectrogram.
Be described in detail the embodiment of the invention below:
The used bacterial blight-resisting material of the present invention DV
85For introducing the anti-source long-grained nonglutinous rice (seeing " rice in China kind and pedigree thereof " .P.276) in the rice varieties. this kind contains Xa-
5.Xa-
7The disease-resistant gene of two different in kinds, performance high resistance to hoja blanca, and disease-resistant stable, high stalk, drape over one's shoulders leaf, integral economic trait is poor, directly utilizes relatively more difficult in breeding for disease resistance.With DV
85With coming (TN No. one in the susceptible variety platform
1) hybridization, transformation becomes semi-short-stalked, the anti-source TN in the centre of high resistance to hoja blanca
1/ DV
85With the anti-source TN in centre
1/ DV
85When being booting, affiliated strain carries out the leaf-cutting inoculation with bacterial strain 300,000,000/ml concentration such as bacterial leaf-blight KS-6-6, Zhejiang 173, the leaf-cutting position is for falling a leaf, minority is for falling two leaves, every strain inoculation 1-3 leaf, inoculating the number of blade in 2 years respectively is 618,129, inoculate back 20 days and pluck the inoculation blade, scab linear measure longimetry and the analysis expanded, the scab average length is respectively 4.65 ± 0.192cm, 3.03 ± 0.206cm.Middle anti-source TN
1/ DV
85Not only showing it has stronger resistance, and has good transformation effect as anti-source donor.
With DV
85Anti-source " TN derives
1/ DV
85" be maternal, hybridize with male parent bright extensive 63, carry out again backcrossing for 3-4 time, breeding recovery is kind " Nan Nong anti-65 "." Nan Nong anti-65 " not only has bright extensive 63 as recovering the integral economic trait advantage that system is had, and resistance, and disease-resistant performance is expressed under male sterile line genetic background effectively, and coordinate force is good, and pollen amount is big, and the florescence is long, the favourable production of hybrid seeds.
Table one:
The total grain of kind plant height individual plant fringe spike length ripening rate thousand kernel weight bacterial leaf-blight
Cm number (cm) % Zhejiang 173
Nan Nong anti-65 103.6 13.43 22.3 137.3 78.77 27.9 0.6
Bright extensive 63 101.3 14.73 21.4 101.9 78.57 28.6 2.1
With male sterile line Zhenshan 97a or imperial Mortopl A or Xieqingzao A is female parent, and recovers system " Nan Nong anti-65 " male parent and hybridizes combo, thereby breed novel combination high resistance to white leaf-blast of hybrid rice kind." Shan excellent anti-65 " plant forms and fertility characteristic are close with " Shanyou 63 ", and be 130-140 days the time of infertility, about plant height 110cm, plant type is concentrated, and blade is bigger, and sword-like leave is soft slightly, tillering ability is strong, property in great numbers is good, and anaphase blade is delicate and pretty, and the big grain of fringe is big, thousand kernel weight 28-31g, rice matter is more excellent, wide adaptability, and yielding ability is good.
Implementing best way of the present invention is with anti-source material DV
85Be male parent, to come (TN No. one in the platform
1) hybridize filial generation " TN for female parent
1/ DV
85" be middle anti-source; Again with TN
1/ DV
85Be female parent, with bright extensive 63 paternal hybrids, backcross and breed for 3-4 time then " Nan Nong anti-65 ", be female parent with male sterile line Zhenshan 97a or imperial Mortopl A or Xieqingzao A at last, to recover system " Nan Nong anti-65 " is that male parent is carried out hybrid seeding, directly applies to large tracts of land production thereby obtain the high resistance to white leaf-blast of hybrid rice new varieties.
Be example with Shan excellent anti-65 below:
Jiangpu, Jiangsu Province county belongs to bacterial leaf-blight Chang Faqu, plants experimentally " Shan excellent anti-65 " in 1991, and test identifies and show that " Shan excellent anti-65 " compares the significantly volume increase (table two, table three) according to (CK):
Table two:
The effective fringe ten thousand of kind plant height cm/every fringe grain husk of mu is spent the solid number of the every fringe of number
Shan excellent anti-65 112.3 16.38 157.5 148.7
Shanyou 63 (CK1) 111.4 16.92 145.4 119.9
Shanyougui 33(CK2) 103.0 16.74 142.7 138.2
Table three:
Kind ripening rate % thousand kernel weight g bacterial leaf-blight level Zhejiang 173 output Kg/ mus
Shan excellent anti-65 93.9 28.6 0.7 697.5
Shanyou 63 (CK1) 83.1 28.1 3.9 576.1
Shanyougui 33(CK2) 89.0 27.3 2.7 631.6
The Jianhu County, Jiangsu Province also belongs to bacterial leaf-blight Chang Faqu, and " Shan excellent anti-65 " planted experimentally in production in 1991, and the result shows that (CK) same significantly volume increase (table four, table five) is shone in " Shan excellent anti-65 " performance comparison:
Table four:
The effective fringe ten thousand of kind plant height cm/every grain husk of mu is spent the solid number of the every fringe of number
Shan excellent anti-65 111.5 16.89 153.4 149.5
Shanyou 63 (CK
1) 110.0 16.74 144.4 118.7
Shanyougui 33(CK
2) 102.0 16.74 141.7 137.2
Table five:
Kind ripening rate % thousand kernel weight (g) bacterial leaf-blight level Zhejiang 173 output Kg/ mus
Shan excellent anti-65 95.1 29.2 0.7 705.6
Shanyou 63 (CK
1) 84.2 27.1 3.9 580.1
Shanyougui 33(CK
2) 89.0 27.3 2.7 632.7
Excellent anti-65 seeding techniques of Shan are identical with Shanyou 63 with culture technique.
Claims (4)
1, a kind of breeding method of high resistance to white leaf-blast of hybrid rice comprises and selects disease-resistant material, and the transformation of anti-source is fastened to the good recovery of comprehensive proterties, and preparing hybrid kind then is characterized in that:
(a), with coming (TN No. one in the platform
1) be maternal, the DV of bacterial blight-resisting
85Be male parent, hybridize, anti-source " TN in the middle of breeding
1/ D
85";
(b), with middle anti-source " TN
1/85" be maternal, bright extensive 63 hybridize for male parent, breed the recovery system " Nan Nong resists 65 " of high resistance to hoja blanca;
(c), be maternal with the male sterile line Zhenshan 97a, recover system " Nan Nong anti-65 " and hybridize, preparing hybrid kind " Shan is excellent to resist 65 " for male parent.
2, breeding method according to claim 1 is characterized in that: A is maternal with male sterile line dragon Mortopl, recovers system " Nan Nong anti-65 " and hybridizes for male parent, preparing hybrid kind " special excellent anti-65 ".
3, breeding method according to claim 1 is characterized in that: with the male sterile line Xieqingzao A is maternal, recovers system " Nan Nong anti-65 " and hybridizes for male parent, and the preparing hybrid kind " assists excellent anti-65 ".
4, breeding method according to claim 1, anti-source " TN in the middle of it is characterized in that
1/ DV
85" hybridize with bright extensive 63 after, carry out 3-4 again and hand over and time to backcross, breed high resistance to hoja blanca and recover system " Nan Nong anti-65 ".
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92103511 CN1028472C (en) | 1992-05-16 | 1992-05-16 | Breeding method for hybrid rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92103511 CN1028472C (en) | 1992-05-16 | 1992-05-16 | Breeding method for hybrid rice |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1066165A CN1066165A (en) | 1992-11-18 |
| CN1028472C true CN1028472C (en) | 1995-05-24 |
Family
ID=4940247
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92103511 Expired - Fee Related CN1028472C (en) | 1992-05-16 | 1992-05-16 | Breeding method for hybrid rice |
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|---|---|
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100589695C (en) * | 2007-06-15 | 2010-02-17 | 广东省农业科学院作物研究所 | Slective seeding method for super-high yield peanut |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1039769C (en) * | 1993-11-10 | 1998-09-16 | 湖南杂交小麦研究中心 | Method for prodn. of double-linear hybrid rice seeds |
| CN1075345C (en) * | 1996-09-18 | 2001-11-28 | 南京农业大学 | Breeding method for high resistance to white leaf-blast of hybrid rice |
| CN101796918A (en) * | 2009-03-12 | 2010-08-11 | 江苏丘陵地区镇江农业科学研究所 | Breeding and seed production methods of quality multi-resistant high-yield hybrid rice |
| CN116602205A (en) * | 2023-05-25 | 2023-08-18 | 福建省农业科学院水稻研究所 | Breeding method of new disease-resistant rice material |
-
1992
- 1992-05-16 CN CN 92103511 patent/CN1028472C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100589695C (en) * | 2007-06-15 | 2010-02-17 | 广东省农业科学院作物研究所 | Slective seeding method for super-high yield peanut |
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| Publication number | Publication date |
|---|---|
| CN1066165A (en) | 1992-11-18 |
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