CN1443440A - Supergrade hybrid rice breeding parent matching method - Google Patents
Supergrade hybrid rice breeding parent matching method Download PDFInfo
- Publication number
- CN1443440A CN1443440A CN 02110994 CN02110994A CN1443440A CN 1443440 A CN1443440 A CN 1443440A CN 02110994 CN02110994 CN 02110994 CN 02110994 A CN02110994 A CN 02110994A CN 1443440 A CN1443440 A CN 1443440A
- Authority
- CN
- China
- Prior art keywords
- rice
- hybrid
- parent
- osculant
- round
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention uses rice intermedium type sterile line as female parent, uses intermedius type restoring line as male parent for cross breeding rice hybrid species. Said invention also includes that uses hsien rice sterile line and intermedius sterile line as female parent and uses rice hsien-keng crossed duplex haploid (DH) population and hsien-keng crossed recombinant inbred line (RIL) population as male parent to make test cross to obtain four hybrid populations of hybrid F1 generation. By detecting character of F1 and using hisen-keng molecule specific probe to detect keng-type index of F1 correspondent to male parent DH and RIL the F1 character-male-type keng-type index correlation can be obtained.
Description
Technical field
The present invention relates to the paddy rice cross breeding breeding technique, particularly paddy rice cross breeding parent's apolegamy.
Background technology
Crop hybrid superiority widely insider is known.China began to carry out rice heterosis utilization research from 1964, production application hybrid rice at first in the world in 1976.Hybrid rice has covered the rice field in the whole nation about 1/2 at present.Being extended to of hybrid rice promotes China's grain to increase production to have played important function year after year.China's major technique characteristic that rice yield increases substantially since the seventies in 20th century is development and has popularized hybrid rice.
Aspect heterosis utilization, producing at present and going up the hybrid rice that utilizes mainly is the intervarietal cross rice, promptly utilizes the interior different cultivars intermolecular hybrid of subspecies (Xian or round-grained rice) to produce hybrid rice.Studies show that genetic polymorphism is little between the interior kind of subspecies, further the limitation of utilizing is bigger, and this also is that the hybrid rice unit yield is in the main cause of pacing up and down in recent years.
Conventional hybridization technique is the major technique of breeding of hybridized rice always.Aspect heterotic prediction, past, most using based on the genetic distance method of form investigation and the method for some Physiology and biochemistries predicted hybrid vigour, as the complementary band method of isodynamic enzyme, chloroplast complementary method, mitochondrial complementation method or the like, but these methods and resultses differ greatly, from Breeding Application, remain in very big distance.
Paddy rice cross breeding comprises the hybridization of male sterile line/maintenance line, male sterile line/recoverys system hybridization, the former hybridize male sterile line, the maintenance line selfing gets maintenance line, the latter hybridize crossbreed (can be used for land for growing field crops production), recovery be selfing must recover be.
Breeding practice shows, on the basis of indica restorer line, adds a certain proportion of japonica rice genetic constitution, can improve the yield level of hybrid rice greatly.Therefore, enlarge the amphilepsis distance, by wide compatibility be used to prepare super high-yielding Hybrids of Indica and Japonica rice and newly make up, just become the common recognition of most of breeding man.But it is low that general Xian round-grained rice mingles kind of ripening rate, hollow kernels, and the ripe phase is slow partially, and the problem that plant uprises has restricted the development and use of inter-subspecies hybrid rice.Because very difficult formation of the hybrid combination between typical long-grained nonglutinous rice and the Typical Japonica produces adaptable super high-yielding potentiality, and mainly male sterile line and the coordination that recovers system in hybrid rice breeding is used, therefore improve the hybrid rice yield level and should break through at first that (parent is meant and is used to hybridize the father from the method for the initiative of breeding material and parent's combo, maternal), handing over strong heterosis utilization and ideotype to mould the Xian round-grained rice combines, be that (" super hybridized rice " this title has arranged to become social custom in the world, and super high-yielding is arranged for the intension place of super hybridized rice breeding, implications such as ideotype).
Summary of the invention
Technical problem to be solved by this invention is to overcome common Xian round-grained rice to mingle kind of ripening rate on the low side, hollow kernels, and the ripe phase is slow partially, the difficult problem that plant uprises provides super hybridized rice breeding parent matching method, to make full use of hybrid vigour, acquisition output height, the hybrid rice that plant type is desirable.
Its special character of the technical solution used in the present invention be sterile with paddy rice osculant be that maternal, osculant recovers be male parent, be used for selection cross paddy rice cross breeding kind.
The preferred middle indica male sterile line of described osculant male sterile line, the indica recovery was in the middle of osculant recovery system was preferred.
Its special character of another technical scheme that the present invention adopts be sterile with paddy rice indica cms line and osculant be that maternal, paddy rice Xian round-grained rice accompanies each other that to hand over RIL (RIL) colony be male parent for a times monoploid (DH) colony and Xian round-grained rice, carry out test cross, obtain four hybrid populations of first familiar generation; Proterties to described F1 detects, and with Xian round-grained rice molecular specific probe in detecting F1 the round-grained rice type index of corresponding male parent DH, RIL, draw F1 proterties～correlation of japonica type Index of male parent; Choose the hybrid strain that required F1 character pair is answered according to this correlation,, be used for selection cross paddy rice cross breeding kind as parental apolegamy.
In paddy rice Xian round-grained rice accompanied each other a times monomer (DH) colony and RIL (RIL) colony, the Xian round-grained rice attribute of each strain system produced differentiation, promptly has different round-grained rice type indexes.The male parent and described two hybridization of female parent of different round-grained rice type indexes, the F1 generation that obtains, show various trait, comprise characters with plant, Other Main Agronomic Characters, yield traits etc.
Choose the hybrid strain that required F1 character pair is answered according to F1 proterties～correlation of japonica type Index of male parent, be meant from F1 is vertical and close proterties or special procure the proterties consideration, select pairing male parent of its superior (this male parent has pairing round-grained rice type index) and female parent.The present invention adopts indica type female parent of paddy rice and osculant female parent.
From general meaning, the final purpose of the paddy rice cross breeding production of hybrid seeds is to obtain desirable crossbreed seed, is used for the land for growing field crops and produces.Paddy rice cross breeding relates to male sterile line, maintenance line, recovery system.When the system of educating can be used for crossbreed that the land for growing field crops produces, with sterile be maternal, recovery is a paternal hybrid, obtains crossbreed F1 and is used for the land for growing field crops and produces; Recovery is that selfing then is restored and is.The breeding of male sterile line be with sterile be maternal, maintenance is a paternal hybrid, obtains male sterile line; The maintenance line selfing then is maintained.
The parent of breeding of hybridized rice of the present invention is meant maternal male sterile line and male parent recovery system.
The present invention adopts osculant male sterile line and osculant to recover system's hybridization, and " osculant " is meant long-grained nonglutinous rice with japonica rice genetic constitution or the japonica rice with long-grained nonglutinous rice genetic constitution, due to its main genetic origin, and indica and middle japonica-cline type in the middle of osculant comprises.
It is maternal that the present invention adopts paddy rice indica cms line and osculant sterile, and accompany each other a times monoploid (DH) colony and RIL (RIL) colony of Xian round-grained rice is male parent, carries out test cross, obtains four hybrid populations.Test cross is meant that in the present invention described two female parents hybridize one by one with two male parents respectively.
Below correlation technique of the present invention is explained:
1, the Xian round-grained rice structure of a times monoploid (DH) colony and RIL (RIL) colony that accompanies each other---
The structure of DH colony:, train the once-seedling forming method by flower and obtain with typical long-grained nonglutinous rice and the hybridization of wide affine japonica rice (wide affine be meant that a rice varieties and long-grained nonglutinous rice and japonica rice hybridization can both be normally solid).In the enforcement, with " special blue or green " and " 0248 " hybridize F1 for seed → plantation F1 for seed, grow up to and when not blooming, get its flower pesticide after the plant booting and cultivate → spend the zygoid (doubled haploid) of training forming seedling through one step culture method → acquisition haplobiont (sterile) → chromosome doubling → can educate.Train the forming seedling through one step culture method about flower: common anther culture is better to the effect of japonica rice, and the plant regeneration frequency of long-grained nonglutinous rice is lower, and the ratio of indica type individuality is very low in the indica-japonica hybrid anther culture descendant; The inventor is through research for many years, and it is traditional 2 to utilize phenylacetic acid to replace in anther culture, and 4-D sets up high-frequency indica-japonica hybrid once-seedling forming system.
The structure of RIL colony: in the invention process, with " special blue or green " and " 0248 " hybridization obtain F1 for seed → plantation F1 for seed, get F2 (selfed seed) → plantation F2 after the results, F2 has separation, results show the seed on the different individual plants respectively, the seed that is F3 (selfed seed) → collect is planted (F4) respectively, continues to select respectively up to stable (F7-F8).
The DH and the RIL colony (male parent) that make up are used for test cross, and are used for the detection of Xian round-grained rice differentiation attribute, are described round-grained rice type index with its round-grained rice type molecular labeling index of Xian round-grained rice molecular specific probe in detecting promptly.
2, hybrid population's plant type and yield traits are measured---
The plant type factor of each strain system of test cross gained hybrid F1 colony and each Other Main Agronomic Characters and yield traits are carried out field observation and indoor investigation, comprise plant sword-like leave form (leaf is long, leaf is wide, with stem angle) and chlorophyll content, plant height, single-strain tassel number, number of grain per ear, ripening rate, thousand kernel weight, individual plant grain yield or the like, wherein characters with plant is observed in maturation and was carried out in preceding 20 days, and Other Main Agronomic Characters and yield traits are investigated when maturation.
3, Xian round-grained rice molecular specific probe in detecting---
Paddy rice Xian round-grained rice molecular specific probe is meant that can distinguish two rice varieties is identical or different standard DNA fragments, prepares.DNA that extracts from different paddy rice and probe are compared, see whether have the dna fragmentation the same with probe, whether the Xian round-grained rice attribute of differentiating two kinds of paddy rice thus is identical.
95 RFLP probes that are used for Xian round-grained rice friendship colony gene mapping of screening further filtered out 31 probes special to the used parent of the present invention before the present invention was before, were used for each strain system of DH colony and RIL colony is detected.
Extract total DNA of each strain system of DH colony and RIL colony during detection, carry out rflp analysis with reference to people's such as McCouch method, probe mark, molecular hyridization and signal detection adopt the ECL detection system.The restriction enzyme of selecting for use is EcoRI and HindIII.Used probe is provided by Tanksley laboratory, U.S. Cornell University.
By detecting, obtain the round-grained rice type molecular labeling index of each strain system of DH colony and RIL colony.The result shows that the Xian round-grained rice attribute of hybridizing constructed DH colony and RIL colony with typical long-grained nonglutinous rice (round-grained rice type molecular labeling index is 0) and Typical Japonica (round-grained rice type molecular labeling index is 1) takes place by very big the separation.
4, F1 proterties～correlation of japonica type Index of male parent analysis---
Round-grained rice type molecular labeling index with above-mentioned gained DH colony and strain system of RIL colony is an abscissa, each correlated traits value of strain system of correspondence hybrid F1 colony is the ordinate mapping, obtain a series of F1 proterties～correlation of japonica type Index of male parent curve map, therefrom male parent " Xian round-grained rice degree " is the influence of described round-grained rice type molecular labeling exponential size to the filial generation correlated traits as can be seen, and obtains being applicable to the parent's of super hybrid rice breeding apolegamy foundation thus.The present invention is based on above-mentioned foundation, it is maternal adopting osculant sterile, and it is male parent that osculant is recovered, and carries out the super hybrid rice breeding.
The present invention, plants experimentally through production and demonstrates application promise in clinical practice so help to select the hybrid rice variety that strong hybrid vigour combines with ideotype owing to selecting for use paddy rice osculant male sterile line and osculant to recover the parent of system as breeding of hybridized rice.It is maternal that the present invention also adopts indica cms line and osculant to recover, with accompany each other times monoploid DH colony and RIL RIL colony of Xian round-grained rice is male parent, carry out test cross and obtain four hybrid populations of first familiar generation, F1 is detected for making correlated traits, and be described round-grained rice type molecular labeling index with the round-grained rice type index of paddy rice Xian round-grained rice molecular specific probe in detecting DH and RIL, draw F1 proterties～correlation of japonica type Index of male parent, so can propose specific direction to the parental apolegamy of breeding of hybridized rice, instruct the used parent's of breeding of hybridized rice apolegamy and seed selection, breeding is significant for super hybrid rice.
Embodiment
Implement Supergrade hybrid rice breeding parent matching method of the present invention, comprising:
1, typical rice variety " special blue or green ", the typical japonica " 02428 " of selecting the process conventional breeding to obtain hybridized special green grass or young crops and 02428, and special green grass or young crops/02428 F1 of plantation is for hybrid.
2, get F1 seville orange flower medicine utilization flower training forming seedling through one step culture method and carry out anther culture, obtain the DH of doubled haploid colony that 131 strains system isozygotys; F2 is for seed in plantation, and the individuality of the existing difference of option table makes up the recombinant inbred lines RIL of 156 strain systems.
3, utilize the doubled haploid colony and the recombinant inbred lines that make up to be male parent, maintenance line of extensive use in the production " the blue or green B early of association " and the wide affine maintenance line of middle indica " 064B " are hybridized respectively for female parent, obtain blue or green B/DH colony early of association, blue or green B/RIL colony early of association and 064B/DH colony, four hybrid populations of 064B/RIL colony.
4, hand over 95 RFLP probes of colony's gene mapping from the Xian round-grained rice that can be used for that one of inventor Zhuan Jieyun recommends, filter out indica type parent " special blue or green " and 31 special probes of round-grained rice type parent " 0248 " to making up genetic group, with these 31 probes each strain system of DH colony and RIL colony is detected, utilization filters out the special green grass or young crops of indica type parent and round-grained rice type parent 0,248 31 special probes are used to detect hybrid strain, calculate round-grained rice type molecular labeling index [round-grained rice type molecular labeling index=round-grained rice phenotypic marker number/(the indica type reference numerals+round-grained rice phenotypic marker number) of each strain system, its value is 〉=0 ,≤1].
5, compare by the Xian round-grained rice attribute that hybridization F1 output, output is constituted the factor and plant type level of factor and its parent, find when the round-grained rice type index of male parent greater than 0.5～0.6 the time, test cross combination output progressively descends, this downward trend is except descending synchronously with ripening rate, also with the minimizing basically identical of single-strain tassel number, being combined in male parent round-grained rice type index is that 0.2～0.6 o'clock output is higher, and round-grained rice type index is generally higher between the output of 0.2～0.5 middle indica parent hybridization combination.No matter be the indica type female parent or the test cross hybrid of osculant female parent, male parent round-grained rice type index 0.1～0.3 o'clock, plant height is on a declining curve, after 0.3, the plant height of the test cross hybrid of osculant female parent is basicly stable state, and the test cross hybrid of indica type female parent uprises greater than 0.5 o'clock plant at male parent round-grained rice type index.Length of blade, width and the area of test cross hybrid top three leaves of osculant female parent almost is the level variation with the round-grained rice type index increase of male parent; The test cross hybrid of indica type female parent slightly increases with the increase of male parent round-grained rice type index, and width of blade is except that falling three leaves, and the sword-like leave and two leaves that fall are wavy, and slightly increase.According to requirement and of the present invention main points of super hybridized rice, the method for optimizing of osculant/osculant for the apolegamy of super hybridized rice breeding parent proposed to output and plant type.
Claims (4)
1, Supergrade hybrid rice breeding parent matching method, it is characterized in that sterile with paddy rice osculant is that female parent, osculant recovery are male parent, is used for selection cross paddy rice cross breeding kind.
2, the method for claim 1, the described osculant of its feature is sterile to be middle indica male sterile line, it is that middle indica recovers system that osculant is recovered.
3, Supergrade hybrid rice breeding parent matching method, it is characterized in that sterile with paddy rice indica cms line and osculant is that maternal, paddy rice Xian round-grained rice accompanies each other that to hand over RIL (RIL) colony be male parent for a times monoploid (DH) colony and Xian round-grained rice, carry out test cross, obtain four hybrid populations of first familiar generation; Proterties to described F1 detects, and with Xian round-grained rice molecular specific probe in detecting F1 the round-grained rice type index of corresponding male parent DH, RIL, draw F1 proterties～correlation of japonica type Index of male parent; Choose the hybrid strain that required F1 character pair is answered according to this correlation,, be used for selection cross paddy rice cross breeding kind as parental apolegamy.
4, method as claimed in claim 3, indica male sterile line in the middle of it is characterized in that described osculant is sterile and being.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02110994XA CN100364385C (en) | 2002-03-07 | 2002-03-07 | Supergrade hybrid rice breeding parent matching method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02110994XA CN100364385C (en) | 2002-03-07 | 2002-03-07 | Supergrade hybrid rice breeding parent matching method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1443440A true CN1443440A (en) | 2003-09-24 |
| CN100364385C CN100364385C (en) | 2008-01-30 |
Family
ID=27811163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB02110994XA Expired - Fee Related CN100364385C (en) | 2002-03-07 | 2002-03-07 | Supergrade hybrid rice breeding parent matching method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100364385C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103109667A (en) * | 2013-02-07 | 2013-05-22 | 廖殷 | Method for breeding super ratooning rice |
| US8604272B2 (en) | 2006-09-28 | 2013-12-10 | Monsanto Technology Llc | Resistance to gray leaf spot in maize |
| US8766035B2 (en) | 2007-08-29 | 2014-07-01 | Monsanto Technology Llc | Methods and compositions for Goss' wilt resistance in corn |
| CN107047282A (en) * | 2017-01-18 | 2017-08-18 | 浙江省农业科学院 | A kind of selection of Indica-Japonica hybrid rice new varieties |
| CN108207611A (en) * | 2017-11-28 | 2018-06-29 | 袁隆平农业高科技股份有限公司 | A kind of selection of crops hybrid new breed |
-
2002
- 2002-03-07 CN CNB02110994XA patent/CN100364385C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10093990B2 (en) | 2006-09-28 | 2018-10-09 | Monsanto Technology Llc | Resistance to gray leaf spot in maize |
| US8604272B2 (en) | 2006-09-28 | 2013-12-10 | Monsanto Technology Llc | Resistance to gray leaf spot in maize |
| US8766035B2 (en) | 2007-08-29 | 2014-07-01 | Monsanto Technology Llc | Methods and compositions for Goss' wilt resistance in corn |
| US10844399B2 (en) | 2007-08-29 | 2020-11-24 | Monsanto Technology Llc | Methods and compositions for Goss' Wilt resistance in corn |
| CN101821409B (en) * | 2007-08-29 | 2014-08-27 | 孟山都技术公司 | Methods and compositions for breeding for preferred traits |
| US9119365B2 (en) | 2007-08-29 | 2015-09-01 | Monsanto Technology Llc | Methods and compositions for Goss' Wilt resistance in corn |
| US10301644B2 (en) | 2007-08-29 | 2019-05-28 | Monsanto Technology Llc | Methods and compositions for Goss' Wilt resistance in corn |
| US9828610B2 (en) | 2007-08-29 | 2017-11-28 | Monsanto Technology Llc | Methods and compositions for Goss' Wilt resistance in corn |
| CN103109667B (en) * | 2013-02-07 | 2014-07-23 | 廖殷 | Method for breeding super ratooning rice |
| CN103109667A (en) * | 2013-02-07 | 2013-05-22 | 廖殷 | Method for breeding super ratooning rice |
| CN107047282B (en) * | 2017-01-18 | 2019-05-28 | 浙江省农业科学院 | A kind of selection of Indica-Japonica hybrid rice new varieties |
| CN107047282A (en) * | 2017-01-18 | 2017-08-18 | 浙江省农业科学院 | A kind of selection of Indica-Japonica hybrid rice new varieties |
| CN108207611A (en) * | 2017-11-28 | 2018-06-29 | 袁隆平农业高科技股份有限公司 | A kind of selection of crops hybrid new breed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100364385C (en) | 2008-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Altman et al. | Introgression of the Glanded‐Plant and Glandless‐Seed Trait from Gossypium sturtianum Willis into Cultivated Upland Cotton Using Ovule Culture 1 | |
| CN108243943B (en) | Method for rapidly improving scab resistance of wheat in Huanghuai region | |
| CN100420368C (en) | Method for selectively breeding hybrid wheat by two-line method utilizing blue grain as marking character | |
| CN88100355A (en) | The improved route that hybrid rice produces | |
| CN110305980B (en) | Breeding method and application of anti-clubroot high-oleic-acid rape | |
| CN113179945A (en) | Breeding method of high-yield lodging-resistant disease-resistant new wheat variety | |
| CN111903500A (en) | Breeding method of fragrant rice blast resistant restorer cinnamon 5886 with high harvest index | |
| CN109042292A (en) | A kind of hybrid rice seed breeding process of same period rice transplanting parent | |
| CN100364385C (en) | Supergrade hybrid rice breeding parent matching method | |
| CN103355163A (en) | Breeding method of disease-resistant watermelon variety | |
| CN100435624C (en) | Breeding method for chilli pepper nuclear male sterile dual purpose line and nuclear substance male sterile recovery line | |
| CN1075346C (en) | Technique for breeding of hybridized rice | |
| CN1843088A (en) | Long-spike neck sterile line selection and breeding method | |
| CN1732748A (en) | A kind of selection of seeds of hybridized tomato | |
| CN102090324A (en) | High-yield and high-quality breeding method for new corn hybrid variety | |
| McFadden et al. | Assessment of Gossypium sturtianum and G. australe as potential sources of Fusarium wilt resistance to cotton | |
| CN1065109C (en) | Method for breeding of wheat seeds resisting wheat chlorisis | |
| CN102433334A (en) | Closely linked molecular marker for novel gene (SS2) of rice sink source | |
| CN1063011C (en) | Method for selective breeding of wheet seeds resisting wheat chlorisis | |
| CN115336510B (en) | Polymerization breeding method of special multi-resistance wheat variety for white spirit starter propagation | |
| CN1659954A (en) | Method for making seeds through hybridizing among three series of paddy rice | |
| CN104396731B (en) | A kind of method for cultivating in precocious, high yield, high-quality short season cotton new lines 425 | |
| CN1041988C (en) | Breeding method of reversible dual-purpose male nucleus sterile line of rice | |
| CN1062410C (en) | Cultural method for optimum restoring line | |
| CN103733982A (en) | Method for breeding tomato parent pure line resistant to tomato late blight |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080130 Termination date: 20180307 |