CN1065109C - Method for breeding of wheat seeds resisting wheat chlorisis - Google Patents
Method for breeding of wheat seeds resisting wheat chlorisis Download PDFInfo
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- CN1065109C CN1065109C CN 96114375 CN96114375A CN1065109C CN 1065109 C CN1065109 C CN 1065109C CN 96114375 CN96114375 CN 96114375 CN 96114375 A CN96114375 A CN 96114375A CN 1065109 C CN1065109 C CN 1065109C
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Abstract
The present invention relates to a new wheat germplasm Y96060 for resisting wheat yellow and dwarf virus disease (BYDY), which is a common wheat-thinopyrum intermedium translocation line obtained by the technology of sexual hybridization and immature embryo cultivation. The new wheat germplasm Y96064 carries the gene for resisting the wheat yellow and dwarf disease on the 2Ai-2 chromosome of thinopyrum intermedium. The resistance of the gene carried on the new wheat germplasm Y96060 is better than that of the resistance gene on the 7Ai-1 chromosome of thinopyrum intermedium. The resistance on the Chinese wheat yellow and dwarf virus GPV, GAV strains and the international PAV strain is high. The translocation line Y96064 of disease resistance can be analyzed by cell heredity, and the chromosome C zonation and hybridization histochemistry technology and the RAPD technology are confirmed.
Description
The present invention is the selection of a kind of anti-yellow stunt of wheat viral disease (BYDV) wheat germplasm, belongs to the biotechnology breeding research field.
Yellow stunt of wheat is the virus disease that is caused by luteovirus (Barley Yellow Dwarf Virus is abbreviated as BYDV), and many in the world wheat main products state often takes a bath agricultural because of the harm of yellow dwarf.According to the U.S. and Australia, the annual evil loss of therefore being critically ill is about 9,500 ten thousand dollars and 3,000 ten thousand dollars; 1976-1993, the annual therefore disease underproduction 5-20% of Chile.NORTHWEST CHINA, northeast and some areas, North China often suffer this evil of being critically ill, serious time underproduction 20-30%.In China spreading trend is arranged in recent years, the harm area expands to the Yellow River and Huai He River, Sichuan, middle and lower reach of Yangtze River Mai Qu.Wheat breeding and production practices prove that most economical, the effective method of preventing and treating the yellow stunt of wheat viral disease is seed selection, popularization disease-resistant variety.Since early fifties begins this disease studied, through extensively identifying, in Triticum, do not find the anti-source of BYDV as yet, the minority disease tolerant variety is only arranged, be difficult to prove effective so utilize in the Triticum genetic resources to carry out the BYDV resistive breeding.The eighties begins that wheat sibling species genus is carried out the BYDV resistance to be identified, finds that tens kinds of wild plants such as couchgrass genus, Agropyron, leymus, goose hat grass genus have the BYDV resistant gene.These genes can suppress the BYDV breeding, thereby to BYDV performance immunity or high anti-.According to research reports, have at least two couples of chromosome 7Ai-1 and 2Ai-2 to contain anti-BYDV gene in the middle couchgrass, and the disease-resistant gene on the 2Ai-2 have stronger resistance than the disease-resistant gene on the 7Ai-1.
Shift the external source resistant gene to common wheat and remove conventional sexual hybridization method, utilize the CSph cryptic mutant, outside the chromosome engineering methods such as wheat list nullisomic, developed the biological technique method-tissue culture that forms since the eighties in addition.The anti-BYDV gene that how middle couchgrass is carried at present imports to common wheat, and the common wheat new germ plasm of creating the first in the world anti-BYDV virus disease is the target that the breeding for disease resistance man pursues always.
Patent applicant of the present invention is in the eighties and the early 1990s, carried out work to the common wheat foreign gene transfer, this achievement paper is delivered in the 8th international wheat genetic association, see also " Proceeding of the Eighth international wheetgenetics symposium (20-25 July 1993, Beijing China P485-488.It is to be female parent with common wheat Wan7107, and wheat-middle couchgrass partial amphidiploid does not have in the awns 4 and is paternal hybrid, and its hybrid F1 backcrosses with 7Wan7107 again, has selected the addition line individual plant with anti-yellow stunt of wheat viral disease from the material of backcrossing.
The objective of the invention is to adopt the method for sexual hybridization and biotechnology, with come from the no awns 4, resistance is better than that 7Ai-1 chromosome carries, the resistant gene that is positioned on the 2Ai-2 chromosome imports wheat, realize the variation of anti-source, enlarge the anti-spectrum of wheat breed, it is single because of anti-source to remedy wheat breed, and resistance is the hidden danger of forfeiture easily.And the further sound technology path that utilizes tissue culture technique to import the external source desirable genes to wheat.
Another object of the present invention is susceptible variety and the hybrid that carries the allos disomic addition line of disease-resistant gene to be carried out rataria cultivate, and by the chromosomal break in the incubation, reclosing and reorganization, creates alien translocation line disease-resistant, genetic stability.The external source chromatin that translocation line contains can divide band and RAPD analysis to differentiate by Chromosomal in situ hybridization, C.
A kind of selection of wheat seeds resisting wheat chlorisis, comprise with common wheat Wan7107 being maternal, in the no awns of wheat-middle couchgrass partial amphidiploid 4 for paternal hybrid, use the wheat-middle couchgrass allos disomic addition line Z of the anti-yellow stunt of wheat viral disease of Wan7107 backcross breeding again
2Use Z
2Be female parent, hybridize for male parent in Shan 7859, and its hybrid rataria carries out tissue culture, makes chromosomal break and reorganization, the anti-yellow stunt of wheat viral disease of seed selection, and have the transposition of chromosome end, chromosome number is the wheat-middle couchgrass translocation line of 2n=42=21 II.
This disease-resistant translocation line Y96060 is the transposition of chromosome end, and chromosome number is the 2n=42=21 II, and genetic stability is good, and economical character is good, is beneficial to the breeding for disease resistance man and utilizes.
Disease-resistant translocation line Y96060 can resist the wheat yellow dwarf virus GPV of China and GAV strain to be, also anti-international PAV strain is.
Disease-resistant translocation line Y96060 identifies that through genetics of resistance its resistant gene is a dominant inheritance, with susceptible variety hybridization F
1Show disease-resistant.
The chromosome of disease-resistant translocation line Y96060 constitutes, and can divide band, in situ hybridization and RAPD technology to obtain confirming by CYTOGENETIC ANALYSIS OF ONE, chromosome C.
Advantage of the present invention and good effect are:
1, realizes the variation of anti-source
Prove that through the inoculation approval test translocation line Y96060 that the present invention obtains is to the wheat yellow dwarf virus GPV of China, GAV strain system, all performance is high anti-to international PAV strain system, is the best common wheat new germ plasm of anti-BYDV in having appeared in the newspapers both at home and abroad at present.
2, translocation line Y96060 of the present invention is better than the disease-resistant germplasm resistance with the anti-BYDV gene of 7Ai-1 chromosome of seed selection.
3, translocation line Y96060 of the present invention is the transposition of chromosome end, and middle couchgrass chromatin content is few, and genetic background is the recommended variety of Chinese wheat main producing region, and economical character is better, is beneficial to the breeding for disease resistance man and utilizes.
4, the tissue culture technique of seed selection process employing of the present invention is quick, easy.
Fig. 1 is the seed selection programme diagram of translocation line Y96060 of the present invention
Fig. 2 shows the 2n=21 II for translocation line of the present invention Y96060 * capital 411 F1 PMC
Fig. 3 is translocation line Y96060 in situ hybridization figure of the present invention
Fig. 4 analyzes collection of illustrative plates for the RAPD of translocation line Y96060 of the present invention
Narrate embodiments of the invention below in conjunction with accompanying drawing:
The selection of a kind of wheat seeds resisting wheat chlorisis Y96060 of the present invention may further comprise the steps:
1, is maternal with common wheat Wan7107, and 4 hybridizes its hybrid F for male parent in the no awns of wheat-middle couchgrass partial amphidiploid
1Use the wheat-middle couchgrass allos disomic addition line Z of the anti-yellow stunt of wheat viral disease of Wan7107 backcross breeding again
2
2, use Z
2Be female parent, hybridize for male parent in Shan 7859, and its hybrid rataria carries out tissue culture, makes spontaneous generation chromosomal break and reorganization, the wheat of the anti-yellow stunt of wheat viral disease of seed selection-middle couchgrass translocation line
(1) 54 rataria is at SD
2On the medium, evoked callus.
(2) nascent callus is at SD
1Successive transfer culture is 2 times on the medium, the green seedling of differentiation on the 1/2MS medium.
Above-mentioned medium SD
1, SD
2Be documented in Acta Genetica Sinica, 23 (5): 376-381, in October, 1996.The MS medium is documented in Murashige, T.﹠F.Shoog (1962) A revisedMedium for growth and bioassays with tobacco tissuecultures.Plant.15:473-497.
(3) obtain regeneration plant 150 strains (on average each rataria regenerate 2.78 strains), transplant survival 123 strains, transplanting survival rate 82%.
3, SC
1Identify and selection for resistance
(1) poison (BYDV GPV strain system) aphid is raised in the field inoculation, observes the record disease resistance after 40 days, records disease-resistant 100 strains, susceptible 23 strains.
(2) select disease-resistant and economical character individual plant 14 strains preferably (code T9417)
(3) select for use susceptible variety capital 411 to carry out test cross (T9417 * capital 411)
4, SC
2Identify and selection for resistance
(1) plantation T9417 selfing strain nursery and test cross combination (T9417 * capital 411)
(2) poison (BYDV GAV strain system) aphid is raised in the field inoculation, observes the record disease resistance after 40 days, selfing strain nursery T9417 ; Record disease-resistant 184 strains, susceptible 32 strains, anti-: feel than greater than 3: 1 test cross combination T9417 * capital 411, record disease-resistant 14 strains, susceptible 9 strains, anti-: the sense ratio was greater than 1: 1.
(3) select to resist: feel plant-Y96060 greater than 3: 1
5, the cytology of disease-resistant translocation line Y96060 and molecular biology identification
Disease-resistant translocation line Y96060 pollen mother cell chromosomes number is 21 II.Root-tip cells chromosome is blockaded with the common wheat genomic DNA, and biotin labeled middle couchgrass genomic DNA carries out in situ hybridization as probe, shows that there is middle couchgrass chromosome segment the dyad end.
6, the economical character of disease-resistant translocation line Y96060
Weak winter habit, long awns, white shell, red grain, in high stalk, in ripe
Claims (1)
1, a kind of selection of wheat seeds resisting wheat chlorisis, comprise with common wheat Wan7107 being maternal, in the no awns of wheat-middle couchgrass partial amphidiploid 4 for paternal hybrid, use the wheat-middle couchgrass allos disomic addition line Z of the anti-yellow stunt of wheat viral disease of Wan7107 backcross breeding again
2It is characterized in that: use Z
2Be female parent, hybridize for male parent in Shan 7859, and its hybrid rataria carries out tissue culture, makes chromosomal break and reorganization, the anti-yellow stunt of wheat viral disease of seed selection, and have the transposition of chromosome end, chromosome number is the wheat-middle couchgrass translocation line of 2n=42=21 II.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96114375 CN1065109C (en) | 1996-12-17 | 1996-12-17 | Method for breeding of wheat seeds resisting wheat chlorisis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96114375 CN1065109C (en) | 1996-12-17 | 1996-12-17 | Method for breeding of wheat seeds resisting wheat chlorisis |
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| Publication Number | Publication Date |
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| CN1185275A CN1185275A (en) | 1998-06-24 |
| CN1065109C true CN1065109C (en) | 2001-05-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96114375 Expired - Fee Related CN1065109C (en) | 1996-12-17 | 1996-12-17 | Method for breeding of wheat seeds resisting wheat chlorisis |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1063011C (en) * | 1996-12-17 | 2001-03-14 | 中国农业科学院作物育种栽培研究所 | Method for selective breeding of wheet seeds resisting wheat chlorisis |
| CN100372934C (en) * | 2003-07-10 | 2008-03-05 | 中国农业科学院作物育种栽培研究所 | Homologous sequence linked with yellow dwarf-resistant gene Bdv2 and use thereof |
| CN100354419C (en) * | 2003-07-25 | 2007-12-12 | 中国农业科学院作物育种栽培研究所 | Wheat yellow dwarf disease candidate resistance gene and its coded protein and uses |
| CN1559186A (en) * | 2004-03-05 | 2005-01-05 | Breeding material and method for breeding by inducing wheat hybrid to producing zygoid plant |
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1996
- 1996-12-17 CN CN 96114375 patent/CN1065109C/en not_active Expired - Fee Related
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