CN102331411A - Blood cell analyzer with blue semiconductor laser - Google Patents
Blood cell analyzer with blue semiconductor laser Download PDFInfo
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- CN102331411A CN102331411A CN201110191878A CN201110191878A CN102331411A CN 102331411 A CN102331411 A CN 102331411A CN 201110191878 A CN201110191878 A CN 201110191878A CN 201110191878 A CN201110191878 A CN 201110191878A CN 102331411 A CN102331411 A CN 102331411A
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Abstract
The invention relates to a blood cell analyzer with a blue semiconductor laser. The blood cell analyzer comprises an analyzer shell, wherein a sample preparation part is arranged on the lower part of the interior of the analyzer shell; a photoelectric sensor is arranged at the top of the interior of the analyzer shell and used for sampling and detecting blood cells which are input by the sample preparation part; a light irradiation unit internally comprises at least one blue semiconductor laser light source; elliptical light spots which are formed after focusing of the light irradiation unit are irradiated on the blood cells, and side scattered lights and fluorescent lights are generated; and a data processing part processes electric signals of a first photoelectric detector and a second photoelectric detector and then outputs a white blood cell detection analysis result. During sampling analysis, forward scattered lights do not participate in cell classification and are not received and detected, so the structure of a detection system is simplified, production cost and maintenance cost are reduced, the stability of the system is improved, the system is compact in structure, convenient to install and use, safe and reliable, and detection accuracy is improved.
Description
Technical field
The present invention relates to a kind of blood cell analyzer, especially a kind of blood cell analyzer with blue semiconductor laser specifically is used for leucocyte is carried out the blood cell analyzer of differential count, belongs to the technical field of blood cell analyzer.
Background technology
Blood cell in the human peripheral blood has three types to be respectively red blood cell, leucocyte and blood platelet, and wherein leucocyte is divided into five types, is respectively eosinophil, neutrophil leucocyte, basophilic granulocyte, lymphocyte, monocyte.In early days blood cell being analyzed all is to rely on manual work to carry out artificial cognition at microscopically through morphology; Coulter had invented a kind of instrument that can analyze automatically blood cell afterwards; Its principle is: particle flux can cause the conductivity at these two ends, hole to change when crossing a full of liquid aperture; The rate of change of conductivity and the volume of particle are proportional; The otherness that so just can utilize the blood cell volume is divided into three types with the blood cell of peripheral blood: leucocyte, red blood cell and blood platelet, because leucocyte volume ratio red blood cell is big from volume, erythrocyte volume is bigger than blood platelet.
Yet the Coulter principle but can't be with leukocytic five sub-category separately, and overlapping because leukocytic five sub-category have on volume, difference is on inner structure.After flow cytometer has been invented by Becton Dikinson company, utilize the principle of optical scattering can the inside result of cell be distinguished, the nineties in last century, a lot of companies were incorporated into low cytometric analysis in the blood cell analyzer one after another; Formation and the modern times five classification blood cell analyzer that leucocyte is carried out five classification, there are Beckman Coulter, ABBOTT in famous company; Sysmex; Siemens, ABX, Mindray.
Patent US6228652 has disclosed a kind of blood cell analyzer device based on flow cytometer, and it utilizes forward direction low angle scattering (LMALS) and forward direction high angle scattered light (UMALS) that leucocyte is carried out the part classification.Patent US2009310122 has disclosed other a kind of blood cell analyzer, utilizes forward scattering light, side scattered light and fluorescence that leucocyte is carried out five classification.Patent US5631165 has disclosed other a kind of five classification blood cell analyzers, has utilized multi-angle polarization and depolarization scattered light to carry out leucocyte five classification.
Along with the development of semiconductor laser (Laser Diode) technology, the little characteristics of long volume of its life-span make it, and place of gas laser instrument and solid state laser appear in flow cytometer and the blood cell analyzer gradually.Patent US6713019 has disclosed a kind of five classification blood cell analyzers that adopt red laser diode as light source.Patent US5788927 also is used in the five classification blood cell analyzers a kind of red laser diode as light source.Above-mentioned blood cell analyzer producer, the SF-3000 of Sysmex company, XT-2000i, XE-2100i have adopted red laser diode as light source; The ADVIA series of Siemens has adopted red laser diode as one of light source; The BC-5500 of Mindray, BC-5300 serial analysis appearance have also adopted red laser diode as light source.
The red laser diode technical development is ripe, and low price is well to select as the driving source of scattered light.If yet need excite fluorescent dye in the analytic system; Then the red laser effect is not good; Because this wave band fluorescent dye than blue wave band lack many; And (Stock shift) is very little for the wavelength that ejects and the difference of irradiates light, is unfavorable for fluorescence and irradiates light are separated, and causes the signal to noise ratio (S/N ratio) of system not high; This also is that most of flow cytometers do not adopt ruddiness as forward scattering and sidewise scattered radiation source, and adopts the reason of the blue light of short-wave band as the radiation source of fluorescence.
Summary of the invention
The objective of the invention is to overcome the deficiency that exists in the prior art, a kind of blood cell analyzer with blue semiconductor laser is provided, its compact conformation; Prolonged serviceable life, improved the signal to noise ratio (S/N ratio) of fluorescence signal, accuracy of detection is high; Applied widely, safe and reliable.
According to technical scheme provided by the invention, said blood cell analyzer with blue semiconductor laser comprises the analyser housing, and the bottom in the said analyser housing is provided with sample preparation portion; Top in the said analyser housing is provided with the photoelectric sensor that is used for the blood cell of sample preparation portion input is carried out sample detecting.
Said photoelectric sensor comprises the flow chamber that allows sheath fluid and sample flow to lead to, and said sample liquid is when flowing in flow chamber under the parcel of sheath fluid, and the blood cell in the sample liquid can pass through the detection zone of flow chamber end one by one; Flow chamber is provided with light irradiation unit and photodetection unit corresponding to the end that detection zone is set; During blood cell in the focal beam spot irradiating and detecting district that said light irradiation unit forms; The photodetection unit can receive scattered light and the fluorescence that produces through blood cell, and said photodetection unit converts scattered light that receives and fluorescence the output of into blood cell analytic signal.
Said flow chamber comprises the chamber body of being processed by optically transparent material, and said chamber body is provided with commutating zone corresponding to the other end that detection zone is set, and the center of said commutating zone is provided with sample liquid input field; Said commutating zone is connected with detection zone through accelerating region.
Hole formation detection zone is detected through being provided with in the center of an end in the body of said chamber, and the axis in said detection hole and the axis of chamber body are located along the same line; Said detection hole is rectangular or square, and the aperture of detecting the hole is 200 μ m ~ 400 μ m.
Said light irradiation unit comprises at least one blue semiconductor LASER Light Source; Be provided with the laser alignment lens and first condenser lens on the light ray propagation direction of said blue semiconductor LASER Light Source successively, the light that the blue semiconductor LASER Light Source penetrates focuses on the center that back focus is positioned at detection zone through the laser alignment lens and first condenser lens.
Said photodetection unit comprises second condenser lens, and said second condenser lens is positioned on the light ray propagation direction perpendicular to light irradiation unit; Second condenser lens is assembled light path and is provided with dichroic beamsplitter, and the reflected light path of said dichroic beamsplitter is provided with second photodetector, and the transmitted light path of dichroic beamsplitter is provided with first photodetector.
Be provided with the long logical color filter and first diaphragm between said first photodetector and dichroic beamsplitter, the contiguous dichroic beamsplitter of the logical color filter of said length; Be provided with second diaphragm between said second photodetector and dichroic beamsplitter.
Said light irradiation unit is radiated at the hot spot ovalize of detection zone center, and the corresponding oval short-axis direction of said hot spot is the flow direction of blood cell in the sample liquid, and said oval minor axis length is less than two leukocytic diameters; The corresponding oval major axis of hot spot is less than the detection zone width.
The output terminal of said photoelectric sensor links to each other with data processing section.
Said sample is prepared part and is comprised the first sampling fixed head and the second sampling fixed head that vertically is distributed in the analyser housing bottom; The said first sampling fixed head is provided with guide rail, and said guide rail is provided with the blood sample sampling thief, and said blood sample sampling thief can move on guide rail; The below of guide rail is provided with reaction tank, and the second sampling fixed head is provided with and is used for sending first syringe in reagent and the fluorochrome sucting reaction pond and with the mixed liquor in the reaction tank into second syringe in the photoelectric sensor.
Advantage of the present invention: inject sheath fluid and sample liquid simultaneously through sample preparation portion in the flow chamber, sample liquid gets into detection zone under sheath fluid parcel and compression, and blood cell passes through detection zone one by one; Comprise at least one blue semiconductor LASER Light Source in the light irradiation unit; The oval hot spot that light irradiation unit focuses on back formation is radiated on the blood cell; And generation side scattered light and fluorescence; Said fluorescence receives through first photodetector and converts electric signal into, and side scattered light receives through second photodetector and converts electric signal into; Data processing division is handled back output leucocyte check and analysis result according to the electric signal of first photodetector and second photodetector; During sampling analysis, forward scattering light is not participated in cytological classification, forward scattering light is not received and surveys, and has simplified the detection system structure; Reduce production and maintenance cost, made system stability improve compact conformation simultaneously; Easy to install, improved accuracy of detection, safe and reliable.
Description of drawings
Fig. 1 is a structured flowchart of the present invention.
Fig. 2 is user mode figure of the present invention.
Fig. 3 is a shape assumption diagram of the present invention.
Fig. 4 is a cut-away view of the present invention.
Fig. 5 is the rear view of Fig. 4.
Fig. 6 is the structured flowchart of photoelectric sensor of the present invention.
Fig. 7 is the structural representation of flow chamber of the present invention.
Fig. 8 is that the A-A of Fig. 7 is to cut-open view.
Fig. 9 is the detection synoptic diagram of flow chamber detection zone of the present invention.
Figure 10 is the functional schematic of dichroic beamsplitter of the present invention.
Figure 11 is a kind of excitation spectrum and spectral radiation curves of bluish violet fluorochrome.
Figure 12 is that the fluorescence of fluorochrome shown in Figure 11 extracts synoptic diagram.
The structural representation that Figure 13 realizes for optical sensing implement body of the present invention.
Figure 14 is the concrete structural representation of realizing in optical sensor internal radiation of the present invention unit.
Figure 15 is flow chamber of the present invention and the concrete structural representation of realizing of light-receiving probe unit.
Description of reference numerals: 1-analyser main frame; The 2-computing machine; The 3-connecting line; 10-analyser housing; The 11-light irradiation unit; The 12-flow chamber; 13-light-receiving probe unit; The 14-hot spot; The 15-blood cell; 20-blood sample sampling thief; The 20a-sampling location; The 20b-response location; The 21-guide rail; The 22-first sampling fixed head; The 30-photoelectric sensor; The 31-substrate; The 32-light source assembly; 33-flow chamber seat; The 34-lens mount; 35-dichroic assembly; 36-first probe assembly; 37-second probe assembly; The 40-reaction tank; 41-sample preparation portion; 51-first syringe; 52-second syringe; The 53-second sampling fixed head; 111-blue semiconductor light source; 112-laser alignment lens; 113-first focus lens group; The 121-commutating zone; The 122-accelerating region; The 123-detection zone; 124-detects the hole; 125-chamber body; 126-sample liquid input field; 131-second condenser lens; The 132-dichroic beamsplitter; The long logical color filter of 133-; 134-first diaphragm; 135-first photodetector; 136-second diaphragm; 137-second photodetector; The 200-data processing section; The 201-sampling needle; 341-first knob; 342-second knob and 343-the 3rd knob.
Embodiment
Below in conjunction with concrete accompanying drawing and embodiment the present invention is described further.
As depicted in figs. 1 and 2: the analyser main frame 1 that the said leucocyte that is used in the blood cell 15 carries out statistic of classification comprises sample preparation portion 41; Said sample preparation portion 41 is used for blood sample and reagent are hatched; To reach the purpose that cell lysis and pair cell carry out fluorescent dye; The blood sample that will hatch be sent in the photoelectric sensor 30 in the analyser main frame 1 after reagent, fluorochrome mix; Leucocyte in 30 pairs of above-mentioned blood samples of photoelectric sensor shines the back and produces side scattered light and fluorescence, and exports after converting said side scattered light and fluorescence into corresponding electric signal, and the signal of photoelectric sensor 30 outputs is input in the data processing section 200; After data processing section 200 statistical study, form the general characteristic of blood sample.Data processing section 200 can be computing machine 2, and said computing machine 2 links to each other with analyser main frame 1 through connecting line 3, forms analytic system.
Like Fig. 3 ~ shown in Figure 5: said analyser main frame 1 comprises analyser housing 10, comprises the first sampling fixed head 22 and the second sampling fixed head 53 that vertically are distributed in analyser housing 10 bottoms in the said analyser housing 10; The said first sampling fixed head 22 is provided with guide rail 21, and said guide rail 21 is provided with blood sample sampling thief 20, and said blood sample sampling thief 20 can move on guide rail 21; The below of guide rail 21 is provided with reaction tank 40, the second sampling fixed heads 53 and is provided with and is used for sending first syringe 51 in reagent and the fluorochrome sucting reaction pond 40 and with the mixed liquors in the reaction tank 40 into the second interior syringe 52 of photoelectric sensor 30.Blood sample sampling thief 20 comprises sampling needle 201, and blood sample sampling thief 20 has sampling location 20a and response location 20b on guide rail 21; When initial, blood sample sampling thief 20 is 20a in the sampling location of guide rail 21, and blood sample sampling thief 20 will be sent in the reaction tank 40 by the blood sample of sucking-off from test tube through sampling needle 201.Blood sample sampling thief 20 is hatched at the reagent that reaction tank 40 neutralizations add in advance, to reach the density that cell lysis and pair cell carry out fluorescent dye.After blood sample is hatched in reaction tank 40; First syringe 51 is through delivering to reagent and fluorochrome suction in the reaction tank 40; After the dyeing, second syringe 52 is sent into the blood sample in the reaction tank 40 in the photoelectric sensor 30, detects through 30 pairs of blood cells 15 of photoelectric sensor.
Like Fig. 1 and shown in Figure 6: said photoelectric sensor 30 comprises light irradiation unit 11, flow chamber 12 and photo detecting unit 13; Allow the circulation of sheath fluid and sample liquid in the said flow chamber 12, when in flow chamber 12, flowing under the parcel of sample liquid at sheath fluid, the cell in the sample liquid can pass through the detection zone 123 of flow chamber 12 ends one by one.Said light irradiation unit 11 and photo detecting unit 13 all are positioned at flow chamber 12 corresponding to the end that detection zone 123 is set, and photo detecting unit 13 is positioned on the optical propagation direction perpendicular to light irradiation unit 11.Focus on light irradiation unit 11 optical propagation directions on the blood cell in the hot spot 14 irradiating and detecting districts 123 that form; Hot spot 14 produces scattered light and fluorescence through blood cell; Photo detecting unit 13 can receive the scattered light and the fluorescence of generation; And convert the scattered light of said reception and fluorescence into the blood cell analytic signal and output to data processing division 200, said data processing division 200 can be accomplished the detection to five types of cells in the leucocyte according to the blood cell analytic signal.Flow chamber 12 is provided with sample preparation portion 41, and sheath fluid and sample liquid through sample preparation portion 41 can control circulation in flow chamber 12 enable to satisfy test requirements.
Like Fig. 7 ~ shown in Figure 10: said flow chamber 12 comprises chamber body 125, rectangular parallelepiped or the cube structure of said chamber body 125 for being processed by optically transparent material.Be respectively equipped with commutating zone 121, accelerating region 122 and detection zone 123 in the chamber body 125, said commutating zone 121 lays respectively at the two ends in the chamber body 125 with detection zone 123, and commutating zone 121 is connected with detection zone 123 through accelerating region 122.The axis of commutating zone 121, accelerating region 122 and detection zone 123 and the axis of chamber body 125 are located along the same line.Detect hole 124 formation detection zones 123 through being provided with in the chamber body 125, the axis in said detection hole 124 and the axis of chamber body 125 are located along the same line, and it is rectangular or square detecting hole 124, and the aperture of detecting hole 124 is 200 μ m ~ 400 μ m.The center of commutating zone 121 is provided with sample liquid input field 126; In chamber body 125, import sample liquid through sample liquid input field 126; Commutating zone 121 is the sheath fluid input field corresponding to the outside of sample liquid input field 126, thereby can wrap up sample liquid through commutating zone 121 back sheath fluids.The outlet of sample liquid input field 126 is the circular hole of diameter 0.3mm; The outlet of commutating zone 121 is an accelerating region 122; Said accelerating region 122 is funnel-form, accelerating region 122 corresponding to the aperture, end in proximity detection district 123 less than the aperture of accelerating region 122 corresponding to contiguous commutating zone 121.The sheath flow that flows in the chamber body 125 will satisfy laminar flow condition, and promptly Reynolds number is less than 2300, and the laminar flow condition of sheath flow can be controlled realization through sample preparation portion 41.Sheath flow and sample liquid are through behind the commutating zone 121; Sheath flow parcel sample liquid is assembled; When getting in the detection zone 123 through accelerating region 122 backs; The sheath flow is compressed to the width less than 2 blood cells with sample liquid, so that the blood cell in the sample liquid can get into one by one and pass through detection zone 123, thereby can realize the detection of single blood cell at every turn.
As shown in Figure 6: said light irradiation unit 11 comprises at least one blue semiconductor LASER Light Source 111, and the bright dipping end of said blue semiconductor LASER Light Source 111 or optical propagation direction end are provided with the laser alignment lens 112 and first condenser lens 113.The laser beam of sending in the blue semiconductor LASER Light Source 111 is through behind laser alignment lens 112 collimations; Focus on respectively on mutually perpendicular both direction through first condenser lens 113, blue semiconductor LASER Light Source 111 focuses on the center that back focus is positioned at the detection zone 123 of flow chamber 12 again.Simultaneously, the hot spot 14 that blue semiconductor LASER Light Source 111 forms in detection zone 123 is oval, and said oval-shaped minor axis is the flow direction of blood cell, and minor axis length is less than two leukocytic diameters; Hot spot 14 is corresponding to forming oval major axis dimension less than the aperture of detecting hole 124, otherwise can shine on the interior rib of flow chamber 12, produces a large amount of parasitic lights, is unfavorable for the detection of signal.
Said photodetection unit 13 comprises second condenser lens 131; Produce fluorescence and scattered light after in flow chamber 12, detecting the irradiation that the blood cell that flows through in the hole 124 receives oval hot spot; Said fluorescence and scattered light are collected by second condenser lens 131 on perpendicular to the optical propagation direction of blue semiconductor LASER Light Source 111, and second condenser lens, 131 collected angular ranges are 70 ~ 100 degree.On the convergence light path of second condenser lens 131, dichroic beamsplitter 132 is set, dichroic beamsplitter 132 can be with the fluorescence transmission of collecting, and can be with the scattered light reflection output of collecting.Therefore, on the fluorescence transmitted light path of dichroic beamsplitter 132, be provided with first photodetector 135, on the reflected light path of dichroic beamsplitter 132, be provided with second photodetector 137.Because fluorescence signal is very faint, in order to reduce the bias light of fluorescence, before getting into first photodetector 135, filter out, to improve the contrast of fluorescence signal with the composition of a long logical color filter 133 with non-fluorescence; Be provided with second diaphragm 134 with logical color filter 133 of duration and 135 of first photodetectors, can eliminate parasitic light, improve signal to noise ratio (S/N ratio) through second diaphragm 134.132 of second photodetector 137 and dichroic beamsplitters are provided with second diaphragm 136.After first photodetector 135 and second photodetector 137 receive fluorescence signal and scattered light signal respectively, convert corresponding light signal into blood cell and analyze electric signal output, and with in the said blood cell analytic signal input data processing division 200.Fluorescence that hot spot 14 is produced according to five types of cells in the blood cell and scattered light different, data processing division 200 can be exported corresponding leukocyte analysis structure.Said second photodetector 137 can adopt photodiode, and first photodetector 135 can adopt photomultiplier.
Fluorescence signal is very faint with respect to scattered light and parasitic light; From scattered light and background miscellaneous light, fluorescence being extracted the synergy that needs dichroscope 132 and long logical color filter 133---scattered light and veiling glare filtering that wavelength is short slightly perhaps reflect away, and the fluorescence that wavelength is long slightly passes through.If wavelength of fluorescence and irradiates light difference of wavelength (stocks shift) then be easy to realize that if stocks shift is very little, then filter effect is very poor.
On blue light and royal purple optical band; The stocks shift of fluorescent dye can be very big; Figure 11 provides excitation spectrum curve and the spectral radiation curves of a kind of fluorescent dye Calcein Violet; Can see that therefrom its stocks shift of this royal purple optical excitation dyestuff reaches 50nm, a kind of feasible fluorescence extraction scheme is with color filter shown in figure 12, and the difficulty of this color filter is also little.And the Stocks shift of the used red fluorescence dyestuff of XT-2000i of Sysmex company and XE-2100 needs the very big color filter of steepness can well extract fluorescence only less than 20nm.Therefore for eliminating fluorescence background, adopt blue laser more to have superiority.Blue semiconductor LASER Light Source 1 of the present invention is a 405nm blue violet semiconductor laser.
Like Figure 13 and shown in Figure 14: be concrete implementation structure synoptic diagram of the present invention.The overall optical electric transducer is positioned on the substrate 31.One end of said substrate 31 is provided with light source assembly 32; Light source assembly 32 comprises blue semiconductor LASER Light Source 111, laser alignment lens 112 and first condenser lens 113; Said light source assembly 32 1 sides are equipped with flow chamber 12 through flow chamber seat 33, and the detection zone 123 of flow chamber 12 is positioned on the propagation light path of light source assembly 32.A side of corresponding flow chamber seat 33 is provided with lens mount 34 on the substrate 31, and said lens mount 34 is perpendicular to light source assembly 32 optical propagation directions; Be provided with second condenser lens 131 in the lens mount 34, lens mount 34 can be done the adjusting of three translational degree of freedom.Substrate 31 is provided with first probe assembly 36 corresponding to the other end that light source assembly 32 and flow chamber seat 33 are set; Said first probe assembly 36 comprises long logical color filter 133, second diaphragm 134 and first photoelectric sensor 8; 34 of first probe assembly 36 and lens mounts are provided with dichroic assembly 35; Be provided with dichroic beamsplitter 132 in the said dichroic assembly 35, the fluorescence printing opacity light path of dichroic beamsplitter 132 is corresponding with first probe assembly 36; The scattered light reflected light path of dichroic beamsplitter 132 is provided with second detector assembly, 37, the second detector assemblies 37 and comprises second diaphragm 136 and second photodetector 137.Wherein light source assembly 32 can be rotated adjusting at substrate 31, with optical system for alignment; Flow chamber seat 33 can carry out one-dimensional translation on substrate regulates, and regulates direction perpendicular to the laser propagation direction; Lens mount 34 is connected with flow chamber seat 33, and can on flow chamber seat 33, carry out three-dimensional translating and regulate; First detector assembly is fixed on the substrate 31 with screw thread; The dichroic assembly is fixed on the substrate 31 with screw thread; Second detector assembly 37 can carry out two-dimensional translation on substrate 31 regulates.Shown in figure 15: as can to carry out three-dimensional translating through first knob 341, second knob 342 and the 3rd knob 343 and regulate.
Like Fig. 1 ~ shown in Figure 15: during use, the light irradiation unit 11 in the adjustment optical sensor 30 and the position of flow chamber 12 make the focal beam spot 14 of light irradiation unit 11 be positioned at detection zone 123 central parts of flow chamber 12.During work, in flow chamber 12, inject sheath fluid and sample liquid through sample preparation portion 41, sample liquid is through in the 126 input flow chambers 12 of sample liquid input field, and sheath fluid is positioned at the outside of sample liquid, can form sheath fluid parcel sample liquid.Behind commutating zone 121 and accelerating region 122, sheath fluid is gone into the indentation of sample hydraulic pressure in the detection zone 123, makes the blood cell in the sample liquid can pass through detection zone 123 one by one.Blue semiconductor LASER Light Source 111 in the light irradiation unit 11 forms focal beam spot 14 through the laser alignment lens 112 and first condenser lens 113 and shines on detection zone 123 hot spot 14 ovalizes.When having blood cell to pass through in the detection zone 123, blood cell can produce side scattered light and fluorescence respectively through after the irradiation of oval hot spot 14.The fluorescence that produces is collected through second condenser lens 131, dichroic beamsplitter 132, long logical color filter 133, first diaphragm 134 and first photodetector 135, and first photodetector 135 converts the fluorescence signal of collecting into electric signal output; Produced simultaneously sideswipe light is collected through second condenser lens 131, dichroic beamsplitter 132, second diaphragm 9 and second photodetector 10, and second photodetector 10 is electric signal output with the side scattered light conversion of signals of collecting.Data processing division 200 receives the electric signal of first photodetector 135 and 10 outputs of second photodetector simultaneously, and different fluorescence and the scattered lights of hot spot 14 generations detected leucocyte according to five classification cells in the different leucocytes; Realized the detection of single blood cell in the sample liquid.After detecting last blood cell, forming statistical property is exactly the general characteristic of this sample liquid.
Inject sheath fluid and sample liquid simultaneously through sample preparation portion 41 in the flow chamber 12 of the present invention, sample liquid gets into detection zone 123 under sheath fluid parcel and compression, and blood cell is one by one through detection zone 123; Comprise at least one blue semiconductor LASER Light Source 111 in the light irradiation unit 11; The oval hot spot 14 that light irradiation unit 11 focuses on back formation is radiated on the blood cell; And generation side scattered light and fluorescence; Said fluorescence receives through first photodetector 135 and converts electric signal into, and side scattered light receives through second photodetector 10 and converts electric signal into; Data processing division 200 is handled back output leucocyte check and analysis result according to the electric signal of first photodetector 135 and second photodetector 10; During sampling analysis, forward scattering light is not participated in cytological classification, forward scattering light is not received and surveys, and has simplified the detection system structure; Reduce production and maintenance cost, made system stability improve compact conformation simultaneously; Easy to install, improved accuracy of detection, safe and reliable.
Claims (10)
1. the blood cell analyzer with blue semiconductor laser comprises analyser housing (10), and the bottom in the said analyser housing (10) is provided with sample preparation portion (41); It is characterized in that: the top in the said analyser housing (10) is provided with the photoelectric sensor (30) that is used for the blood cell (15) of sample preparation portion (41) input is carried out sample detecting.
2. the blood cell analyzer with blue semiconductor laser according to claim 1; It is characterized in that: said photoelectric sensor (30) comprises the flow chamber (12) that allows sheath fluid and sample flow to lead to; Said sample liquid is when flowing in flow chamber (12) under the parcel of sheath fluid, and the blood cell (15) in the sample liquid can pass through the detection zone (123) of flow chamber (12) end one by one; Flow chamber (12) is provided with light irradiation unit (11) and photodetection unit (13) corresponding to the end that detection zone (123) is set; During blood cell in focal beam spot (14) the irradiating and detecting district (123) that said light irradiation unit (11) forms; Photodetection unit (13) can receive scattered light and the fluorescence that produces through blood cell, and said photodetection unit (13) converts scattered light that receives and fluorescence the output of into blood cell analytic signal.
3. the blood cell analyzer with blue semiconductor laser according to claim 2; It is characterized in that: said flow chamber (12) comprises the chamber body of being processed by optically transparent material (125); Said chamber body (125) is provided with commutating zone (121) corresponding to the other end that detection zone (123) is set, and the center of said commutating zone (121) is provided with sample liquid input field (126); Said commutating zone (121) is connected with detection zone (123) through accelerating region (122).
4. the blood cell analyzer with blue semiconductor laser according to claim 2; It is characterized in that: hole (124) formation detection zone (123) is detected through being provided with in the center of an end in the said chamber body (125), and the axis of the axis in said detection hole (124) and chamber body (125) is located along the same line; Said detection hole (124) is rectangular or square, and the aperture of detecting hole (124) is 200 μ m ~ 400 μ m.
5. the blood cell analyzer with blue semiconductor laser according to claim 2; It is characterized in that: said light irradiation unit (11) comprises at least one blue semiconductor LASER Light Source (111); Be provided with laser alignment lens (112) and first condenser lens (113) on the light ray propagation direction of said blue semiconductor LASER Light Source (111) successively, the light that blue semiconductor LASER Light Source (111) penetrates focuses on the center that back focus is positioned at detection zone (123) through laser alignment lens (112) and first condenser lens (113).
6. the blood cell analyzer with blue semiconductor laser according to claim 2; It is characterized in that: said photodetection unit (13) comprises second condenser lens (131), and said second condenser lens (131) is positioned on the light ray propagation direction perpendicular to light irradiation unit (11); Second condenser lens (131) is assembled light path and is provided with dichroic beamsplitter (132); The reflected light path of said dichroic beamsplitter (132) is provided with second photodetector (137), and the transmitted light path of dichroic beamsplitter (132) is provided with first photodetector (135).
7. the blood cell analyzer with blue semiconductor laser according to claim 6; It is characterized in that: be provided with long logical color filter (133) and first diaphragm (134) between said first photodetector (135) and dichroic beamsplitter (132), said length is led to the contiguous dichroic beamsplitter (132) of color filter (133); Be provided with second diaphragm (136) between said second photodetector (137) and dichroic beamsplitter (132).
8. the blood cell analyzer with blue semiconductor laser according to claim 2; It is characterized in that: said light irradiation unit (11) is radiated at hot spot (14) ovalize of detection zone (123) center; The flow direction that the corresponding oval short-axis direction of said hot spot (14) is a blood cell in the sample liquid, said oval minor axis length is less than two leukocytic diameters; The corresponding oval major axis of hot spot (14) is less than detection zone (123) width.
9. the blood cell analyzer with blue semiconductor laser according to claim 1 is characterized in that: the output terminal of said photoelectric sensor (30) links to each other with data processing section (200).
10. the blood cell analyzer with blue semiconductor laser according to claim 1 is characterized in that: said sample is prepared part (41) and is comprised the first sampling fixed head (22) and the second sampling fixed head (53) that vertically is distributed in analyser housing (10) bottom; The said first sampling fixed head (22) is provided with guide rail (21), and said guide rail (21) is provided with blood sample sampling thief (20), and said blood sample sampling thief (20) can be gone up at guide rail (21) and move; The below of guide rail (21) is provided with reaction tank (40), and the second sampling fixed head (53) is provided with and is used for sending first syringe (51) in reagent and the fluorochrome sucting reaction pond (40) and with the mixed liquor in the reaction tank (40) into second syringe (52) in the photoelectric sensor (30).
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| CN201110191878A CN102331411A (en) | 2011-07-08 | 2011-07-08 | Blood cell analyzer with blue semiconductor laser |
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| CN201110191878A CN102331411A (en) | 2011-07-08 | 2011-07-08 | Blood cell analyzer with blue semiconductor laser |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103439523A (en) * | 2013-09-09 | 2013-12-11 | 陶靖 | Device and method for sample treatment and particle analysis |
| CN103823073A (en) * | 2013-06-03 | 2014-05-28 | 陶靖 | Automatic device for sample treatment and particle analysis, and method for sample treatment and particle analysis |
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| CN103823073A (en) * | 2013-06-03 | 2014-05-28 | 陶靖 | Automatic device for sample treatment and particle analysis, and method for sample treatment and particle analysis |
| CN103439523A (en) * | 2013-09-09 | 2013-12-11 | 陶靖 | Device and method for sample treatment and particle analysis |
| CN103940793A (en) * | 2014-03-28 | 2014-07-23 | 北京理工大学 | Fluorescence detection system and detection method |
| CN103940793B (en) * | 2014-03-28 | 2016-05-04 | 北京理工大学 | Fluorescence detecting system and detection method |
| CN114062231A (en) * | 2015-10-28 | 2022-02-18 | 国立大学法人东京大学 | Analysis device |
| WO2020146967A1 (en) * | 2019-01-14 | 2020-07-23 | 深圳迈瑞生物医疗电子股份有限公司 | Optical test device for sample |
| CN113015903B (en) * | 2019-06-11 | 2024-05-14 | 深圳迈瑞生物医疗电子股份有限公司 | Sample detection method and sample analyzer |
| CN113015903A (en) * | 2019-06-11 | 2021-06-22 | 深圳迈瑞生物医疗电子股份有限公司 | Sample detection method and sample analyzer |
| WO2021042307A1 (en) * | 2019-09-04 | 2021-03-11 | 深圳迈瑞生物医疗电子股份有限公司 | Blood testing method and blood analysis system |
| CN110964630A (en) * | 2019-12-18 | 2020-04-07 | 武汉友芝友医疗科技股份有限公司 | Device and method for detecting liquid separation completeness in cell filter |
| CN113624644A (en) * | 2020-05-08 | 2021-11-09 | 深圳迈瑞生物医疗电子股份有限公司 | Optical detection system and blood cell analyzer |
| CN111982790A (en) * | 2020-08-24 | 2020-11-24 | 深圳唯公生物科技有限公司 | Weak light analysis system and method for improving performance of cell and particle analyzer |
| CN114216850A (en) * | 2021-11-04 | 2022-03-22 | 万贝医疗健康科技(上海)有限公司 | Lung cancer cell activity state assessment equipment after radiotherapy and chemotherapy |
| WO2024007774A1 (en) * | 2022-07-06 | 2024-01-11 | 深圳迈瑞生物医疗电子股份有限公司 | Particle detection device and sample analyzer |
| WO2024007773A1 (en) * | 2022-07-06 | 2024-01-11 | 深圳迈瑞生物医疗电子股份有限公司 | Particle detection device and sample analyzer |
| WO2024007775A1 (en) * | 2022-07-06 | 2024-01-11 | 深圳迈瑞生物医疗电子股份有限公司 | Particle detection device and sample analyzer |
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