WO2020146967A1

WO2020146967A1 - Optical test device for sample

Info

Publication number: WO2020146967A1
Application number: PCT/CN2019/071543
Authority: WO
Inventors: 汪东生
Original assignee: 深圳迈瑞生物医疗电子股份有限公司
Priority date: 2019-01-14
Filing date: 2019-01-14
Publication date: 2020-07-23
Also published as: WO2020147255A1; CN113196039A

Abstract

An optical test device for a sample, comprising: a flow chamber (6) for allowing cells in a sample to be tested to pass through one by one; a light source (1) for irradiating the cells passing through the flow chamber (6); a first forward scattered light signal collection assembly (20) for collecting forward scattered light within a first angular range, the forward scattered light within the first angular range being reflected light which is generated by irradiating, by means of the light source (1), the cells passing through the flow chamber (6); and a second forward scattered light signal collection assembly (30) for directly collecting forward scattered light within a second angular range, the forward scattered light within the second angular range being light which is generated by irradiating, by means of the light source (1), the cells passing through the flow chamber (6).

Description

Sample optical detection device

Technical field

The invention relates to a sample optical detection device.

Background technique

Most blood cell analyzers use the principle of laser scattering for measurement. The principle is: irradiate the laser on the cell, collect the forward scattered light, side scattered light (90 degree scattered light) and side fluorescence (90 degree scattered light) produced by the cells after being irradiated. Degree fluorescence) to classify and count cells.

Figure 1 is an optical detection device of a blood cell analyzer. Cells pass through the flow chamber one by one under the action of sheath flow. When the light emitted by the laser light source is collimated by the lens, it irradiates the cells passing through the flow chamber, and the light irradiates the cells. It will scatter to the surroundings. After collecting the forward scattered light through a collecting lens, it passes through an aperture to limit the angle of the forward scattered light that finally reaches the photodetector. For example, the forward scattered light is limited to a low angle (or Speaking of small angle) forward scattered light-this angle of forward scattered light is generally used to measure cell volume; at the same time, the side light is collected through another collecting lens in the direction perpendicular to the light irradiated to the cell. The side light is reflected and refracted by the dichroic mirror, and the side scattered light in the side light is reflected when passing through the dichroic mirror, and then reaches the corresponding photodetector-the side scattered light is generally used To measure the surface complexity of the cell, the side fluorescence in the side light is refracted or transmitted, and then passes through a filter to reach the corresponding photodetector. The side fluorescence is generally used to measure the nucleic acid content in the cell.

The optical detection device in Figure 1 only has three measurement channels—that is, a low-angle forward scattered light channel, a side scattered light channel, and a side fluorescence channel. Therefore, the cells can only be measured based on the signals obtained by these three measurement channels. Classification and counting, which will limit the further scoring and counting of cells to a certain extent, that is, it is impossible to perform more dimensional and more detailed classification and counting, which reduces the classification ability of abnormal cells; The angular forward scattered light channel replaces or adds a high-angle (or large-angle) scattered light channel. You can directly use the photodetector target surface to receive large-angle forward scattered light, but the signal-to-noise ratio obtained in this way is very poor. Therefore, in order to ensure signal quality, technicians usually use a complex combination of multiple lenses to collect large-angle forward scattered light and then emit it to the corresponding photodetector. This approach will greatly increase the cost of the device; in addition, optical detection The size of the device is generally too large. This is due to the selection of the optical path structure. For example, the forward scattered light channel is generally designed as a refractive optical path structure, so this will cause the size of the optical detection device to be too large, especially for the current direction. The scattered light channel is used to collect scattered light in multiple angle ranges (such as low angle and high angle, etc.).

technical problem

The present invention mainly provides a sample optical detection device, which is described below.

Technical solution

The sample optical detection device of an embodiment includes:

Flow chamber for the cells in the sample to be tested to pass through one by one;

A light source for illuminating the cells passing through the flow chamber;

The first forward scattered light signal collection component is used to collect forward scattered light in a first angular range, and the forward scattered light is the reflected light generated by the light source irradiating cells passing through the flow cell;

The second forward scattered light signal collecting component is used to directly collect forward scattered light in the second angular range, and the forward scattered light is light generated by the light source irradiating cells passing through the flow cell.

In an embodiment, the sample optical detection device further includes a reflection light blocking component, which is arranged on the optical path of the forward scattered light generated by the cells passing through the flow cell irradiated by the light source, for reflecting forward scattered light in the first angular range To the first forward scattered light signal collection component, and allow the forward scattered light of the second angle range to directly enter the second forward scattered light signal collection component.

In an embodiment, the reflective light blocking component includes a reflecting mirror for reflecting forward scattered light in a first angular range to the first forward scattered light signal collection component.

In one embodiment, the reflector is elliptical.

In an embodiment, the reflective light-shielding component further includes a light-shielding strip for shielding stray light other than the forward scattered light in the second angular range; wherein the reflector is arranged on the light-shielding strip.

In one embodiment, the reflector is arranged on the light-shielding strip in such a way that its long axis coincides with the light-shielding strip.

In an embodiment, the sample optical detection device further includes a lens assembly, which is arranged on the optical path of the forward scattered light generated by the cells passing through the flow cell irradiated by the light source, and is used to collect the light in the first and second angular ranges The light is scattered forward and emitted to the reflective light blocking component.

In an embodiment, the lens assembly includes one aspheric lens and one spherical lens, or, multiple aspheric lenses, or multiple spherical lenses, or, one aspheric lens and multiple spherical lenses.

In an embodiment, when the transparent component includes a spherical lens, the effective data aperture of the spherical lens closest to the flow chamber is at least 0.34.

In an embodiment, the sample optical detection device further includes a straight-blocking diaphragm, which is arranged between the lens assembly and the reflective light-blocking assembly, and is used to block the direct-angle light in the forward scattered light emitted by the lens assembly, And/or, the forward scattered light emitted by the lens assembly is limited to the first angle range and the second angle range.

In an embodiment, the first forward scattered light signal collection component includes a first angle limiting diaphragm, a stray light blocking diaphragm, and a photodetector which are sequentially arranged; the first angle limiting diaphragm is used to transmit the reflected light The forward scattered light is limited in the first angular range and converges on the stray light stop, the stray light stop is used to shield the stray light of the forward scattered light in the first angular range, and the photodetector is used To convert the collected forward scattered light in the first angular range into an electrical signal.

In an embodiment, the second forward scattered light signal collection component includes a stray light blocking diaphragm and a photodetector arranged in sequence, and the stray light blocking diaphragm is used to shield the forward scattered light in the second angular range. Stray light, the photodetector is used to convert the collected forward scattered light in the second angular range into an electrical signal.

In an embodiment, the sample optical detection device further includes a third forward scattered light signal collection component for collecting forward scattered light in a third angular range, and the forward scattered light is irradiated by the light source through the flow chamber. Light produced by cells and reflected at least once.

In an embodiment, the third forward scattered light signal collection component includes a mirror, a third angular range aperture diaphragm, and a photodetector which are sequentially arranged; the mirror is used to irradiate the light source through the flow cell The forward scattered light of the third angular range generated by the cell is reflected to the third angular range aperture diaphragm, and the third angular range aperture diaphragm is used to limit the forward scattered light in the third angular range, and the photoelectric The detector is used to convert the collected forward scattered light in the third angular range into an electrical signal.

In an embodiment, the first angle range is a low angle range, and/or the second angle range is a medium angle range, and/or the third angle range is a high angle range.

In an embodiment, the first angle range and the second angle range are continuous ranges.

In an embodiment, the first angle range, the second angle range, and the third angle range are continuous ranges.

In an embodiment, the first angle range is 0 to 10 degrees or 1 degree to 10 degrees; and/or, the second angle range is 10 degrees to 20 degrees; and/or, the third angle range It is 20 degrees to 70 degrees.

In an embodiment, the sample optical detection device further includes a light source shaping component for collimating the light beam emitted by the light source and making it converge on the cells passing through the flow chamber.

In an embodiment, the light source shaping assembly includes a collimating lens and a first cylindrical lens arranged in sequence, the collimating lens is used to collimate the light beam emitted by the light source, and the first cylindrical lens is used for To make the light beam converge at the center of the flow chamber in the direction in which the cells pass.

In an embodiment, the sample optical detection device further includes a second cylindrical mirror arranged on the exit light path of the first cylindrical mirror, for converging the light beam in a direction perpendicular to the cell passing through, so that the scattered light Are illuminated into the straight stop diaphragm.

In an embodiment, the sample optical detection device further includes an optical isolator disposed between the collimating lens and the first cylindrical mirror to suppress feedback light.

In an embodiment, the sample optical detection device further includes:

The side scattered light signal collection component is used to collect the side scattered light generated by the light source irradiating cells passing through the flow chamber; and/or,

The lateral fluorescence signal collection component is used to collect the lateral fluorescence generated by the light source irradiating cells passing through the flow chamber.

Beneficial effect

According to the sample optical detection device of the foregoing embodiment, the first forward scattered light signal collection component collects the reflected forward scattered light in the first angular range, and the second forward scattered light signal collection component directly collects or collects the refracted forward scattered light. Or the transmitted forward scattered light, through the design of this optical structure, the longitudinal size of the optical path can be compressed, so that the designed sample optical detection device can be miniaturized. .

BRIEF DESCRIPTION

Figure 1 is a schematic structural diagram of an optical detection device of a blood cell analyzer;

2 is a schematic diagram of the structure of a sample optical detection device in an embodiment;

FIG. 3 is a schematic diagram for explaining the structure of a first forward scattered light signal collection component and a second forward scattered light signal collection component;

4 is a schematic diagram for explaining the structure of the reflective light blocking component;

FIG. 5 is a schematic structural diagram of a sample optical detection device including a lens assembly in an embodiment;

Figure 6 is a schematic diagram of a structure for a lens assembly;

FIG. 7 is a schematic structural diagram of a sample optical detection device including a straight diaphragm in an embodiment;

8 is a schematic structural diagram of a sample optical detection device including a third forward scattered light signal collection component in an embodiment;

FIG. 9 is a schematic diagram for explaining the structure of a third forward scattered light signal collecting component;

10 is a schematic structural diagram of a sample optical detection device including a side scattered light signal collection component and a side fluorescence signal collection component in an embodiment;

FIG. 11 is a schematic diagram for explaining the structure of the side scattered light signal collecting component and the side fluorescent signal collecting component;

Fig. 12 is a schematic structural diagram of a sample optical detection device including a light source shaping component in an embodiment;

FIG. 13 is a schematic structural diagram of a sample optical detection device according to another embodiment.

Embodiments of the invention

detailed description

The present invention will be further described in detail below through specific embodiments and drawings. Among them, similar elements in different embodiments use related similar element numbers. In the following embodiments, many detailed descriptions are used to make this application better understood. However, those skilled in the art can easily realize that some of the features can be omitted under different circumstances, or can be replaced by other elements, materials, and methods. In some cases, some operations related to this application are not shown or described in the specification. This is to prevent the core part of this application from being overwhelmed by excessive descriptions. For those skilled in the art, these are described in detail. Relevant operations are not necessary. They can fully understand the relevant operations based on the description in the manual and general technical knowledge in the field.

In addition, the features, operations, or features described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be sequentially exchanged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and the drawings are only for the purpose of clearly describing a certain embodiment, and are not meant to be a necessary order, unless it is otherwise stated that a certain order must be followed.

The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the described objects and do not have any sequence or technical meaning. The "connection" and "connection" mentioned in this application include direct and indirect connection (connection) unless otherwise specified.

2, an embodiment of the present invention provides a sample optical detection device, the sample optical detection device includes a light source 1, a flow chamber 6, a first forward scattered light signal collection component 20 and a second forward scattered light signal The collection component 30 is described in detail below.

The flow chamber 6 is used for the cells in the sample to be tested to pass one by one. For example, after the blood cells in the sample to be tested are dissolved or stained with some reagents, the sheath flow technology is used to make the blood cells queue through the flow chamber 6 one by one. The Y-axis direction in the figure is the direction of blood cell movement in the sample. It should be noted that the Y-axis direction in the figure is a direction perpendicular to the paper.

The light source 1 is used to illuminate the cells passing through the flow chamber 6. In one embodiment, the light source 1 is a laser, such as a helium-neon laser or a semiconductor laser. When the light emitted by the light source 1 irradiates the cells in the flow chamber 6, it will scatter to the surroundings. In an embodiment of the present invention, the first forward scattered light signal collection component 20 and the second forward scattered light signal collection component 30 are respectively used to collect forward scattered light in the first angular range and forward scattered light in the second angular range, This will be explained in detail below.

The first forward scattered light signal collection component 20 is used to collect forward scattered light in a first angular range, and the forward scattered light is the reflected light generated by the light source 1 irradiating the cells passing through the flow cell 6. 3, in one embodiment, the first forward scattered light signal collection component 20 includes a first angle limiting diaphragm 21, a stray light blocking diaphragm 22, and a photodetector 23 arranged in sequence; the first angle limiting diaphragm is used To limit the reflected forward scattered light in the first angular range and converge on the stray light stop 22, the stray light stop 22 is used to shield the stray light of the forward scattered light in the first angular range, and photodetection The device 23 is used to convert the collected forward scattered light in the first angular range into an electrical signal. In an embodiment, the first angle range is a low angle range, for example, the first angle range is 0 to 10 degrees or 1 degree to 10 degrees.

The second forward scattered light signal collection component 30 is used to collect forward scattered light in a second angular range, and the forward scattered light is light generated by the light source 1 irradiating cells passing through the flow cell 6. In one embodiment, the second forward scattered light signal collection component 30 includes a stray light blocking diaphragm 31 and a photodetector 32 which are sequentially arranged, and the stray light blocking diaphragm 31 is used to shield the forward scattered light in the second angular range. Stray light, the photodetector 32 is used to convert the collected forward scattered light in the second angular range into an electrical signal. In an embodiment, the second angle range is a middle angle range, for example, the second angle range is 10 degrees to 20 degrees. In an embodiment, the first angle range and the second angle range are continuous ranges.

The first forward scattered light signal collection component 20 collects the reflected forward scattered light in the first angular range, and the second forward scattered light signal collection component 30 directly collects or collects the refracted or transmitted forward scattered light, Through the design of this optical structure, the longitudinal dimension of the optical path (the Z axis direction in the figure) can be compressed, so that the designed sample optical detection device can be miniaturized.

In an embodiment of the present invention, a reflective light blocking component is used to spatially separate the forward scattered light in the first angular range and the second angular range, and one is reflected to the first forward scattered light signal collection component 20 , One is allowed to pass through to the second forward scattered light signal collection component 30, which will be described in detail below.

The sample optical detection device in an embodiment further includes a reflective light blocking component 10, which is disposed on the optical path of the forward scattered light generated by the light source 1 irradiating the cells passing through the flow chamber 6, and is used to adjust the first angle range The forward scattered light is reflected to the first forward scattered light signal collection component 20, and the forward scattered light in the second angular range is allowed to directly enter the second forward scattered light signal collection component 30. Please refer to FIG. 4(a) There are many implementations of the reflective light blocking assembly 10. For example, in one embodiment, the reflective light blocking assembly 10 includes a mirror 11 for reflecting forward scattered light in a first angular range to the first A forward scattered light signal collection component 20. In one embodiment, the reflector 11 may be elliptical, that is, the reflector 11 is an elliptical aperture reflector. The placement angle of the reflector 11 can be 45 degrees. At this time, the mirror surface is parallel to the Y axis in the figure. Of course, the placement angle of the reflector 11 can also be adjusted according to the optical path spatial layout, and is not limited to only 45 degrees. Please refer to FIG. 4( b ). In one embodiment, the reflective light blocking component 10 includes a light blocking strip 12 for shielding stray light except for the forward scattered light in the second angle range. In one embodiment, the reflector 11 is arranged on the light-shielding strip 12. For example, when the reflector 11 is elliptical, the reflector 11 is arranged on the light-shielding strip 12 in such a way that its long axis coincides with the light-shielding strip 12, as shown in Figure 4 (c ) As shown. The shading strip 12 here acts as a support for the reflector 11, and the other is to shield stray light except for the forward scattered light in the second angle range, which can effectively ensure the signal quality of the forward scattered light in the second angle range. .

In order to further improve the signal quality of the forward scattered light in the first angle range and the second angle range, please refer to FIG. 5. In an embodiment, the sample optical detection device may further include a lens assembly 13, which is arranged in the light source 1 to illuminate The forward scattered light generated by the cells in the flow chamber 6 is used to collect the forward scattered light in the first angular range and the second angular range on the optical path, and emit the forward scattered light to the reflective light blocking assembly 10. For example, in one embodiment, the lens assembly 13 includes one aspheric lens and one spherical lens, or, multiple aspheric lenses, or multiple spherical lenses, or, one aspheric lens and multiple spherical lenses. Please refer to FIG. 6, an example in which the lens assembly 13 can be composed of two spherical lenses 14 and 15 in a specific embodiment. In an embodiment, when the lens assembly 13 includes a spherical lens, the effective data aperture of the spherical lens closest to the flow chamber 6 is at least 0.34. The introduction of the lens assembly 13 can ensure that the system aberrations are effectively corrected, and that the signal spot can be effectively collected by the two measurement channels of the first forward scattered light signal collection component 20 and the second forward scattered light signal collection component 30 . In addition, the combination of the lens assembly 13 and the reflective light blocking assembly 10 can effectively ensure the signal quality of the forward scattered light in the first angular range.

Referring to FIG. 7, in order to further improve the signal quality of the forward scattered light in the first angle range and the second angle range, in an embodiment, the sample optical detection device may further include a straight stop 16 arranged at the lens assembly 13 and Reflective light blocking components 10 between. The straight-blocking diaphragm 16 has many functions. For example, the straight-blocking diaphragm 16 can block the direct angle of 0 degree light in the forward scattered light emitted by the lens assembly 13-this can prevent signal saturation, and/or can prevent the lens The forward scattered light emitted by the component 13 is limited to the first angle range and the second angle range.

The above is some descriptions of how the sample optical detection device can collect forward scattered light in the first angle range and the second angle range. In some embodiments, the sample optical detection device can also collect forward scattered light in the third angle range. For example, in an embodiment, the third angle range is a high angle range, for example, the third angle range is 20 degrees to 70 degrees. In some embodiments, the first angle range, the second angle range, and the third angle range are continuous ranges. The following specifically describes how the sample optical detection device collects forward scattered light in the third angular range.

Considering that the optical path space behind the flow chamber 6 in the sample optical detection device is relatively tight-for example, it is provided with a first forward scattered light signal collection component 20 and a second forward scattered light signal collection component 30, and even a lens Components 13 and so on. Therefore, if a photodetector is used to directly collect forward scattered light, it cannot guarantee that the forward scattered light in the third angle range (for example, high angle range) can be completely collected. Therefore, the inventor considers the optical path structure for refraction To convert the forward scattered light in the third angle range (for example, the high angle range), so that the forward scattered light in the third angle range (for example, the high angle range) can be effectively collected.

Therefore, please refer to FIG. 8. In one embodiment, the sample optical detection device further includes a third forward scattered light signal collection component 40 for collecting forward scattered light in a third angular range, and the forward scattered light is irradiated by the light source 1 The light generated by the cells of the flow chamber 6 and reflected at least once. Referring to FIG. 9, in an embodiment, the third forward scattered light signal collection component 40 includes a mirror 41, a third angular range aperture stop 42 and a photodetector 43 arranged in sequence; the mirror 41 is used to connect the light source 1 The forward scattered light of the third angular range generated by the cells irradiated through the flow cell 6 is reflected to the third angular range aperture stop 42, and the third angular range aperture stop 42 is used to limit the forward scattered light to the third angular range The photodetector 43 is used to convert the collected forward scattered light in the third angular range into an electrical signal. It should be noted that the sample optical detection device shown in FIG. 9 is only an example drawn to illustrate the third forward scattered light signal collection component 40, which does not represent the sample optical detection device including the third forward scattered light signal collection component 40 The detection device can only have the structure shown in FIG. 9.

The above is some description of the collection of forward scattered light by the sample optical detection device. In some embodiments, the sample optical detection device of the present invention can also realize the detection of side light, such as side scattered light and/or side fluorescence. Collection is described in detail below with reference to Figure 10 and Figure 11.

The sample optical detection device of an embodiment further includes a side scattered light signal collection assembly 50 for collecting side scattered light generated by the light source 1 irradiating the cells passing through the flow chamber 6. In one embodiment, the side scattered light signal collection component 50 includes a side small aperture diaphragm 51 and a photodetector 52 arranged in sequence. The side small aperture diaphragm 51 is used to process lateral scattered light, and the photodetector 52 receives The lateral dispersion light processed by the lateral aperture diaphragm 51 is converted into an electrical signal.

The sample optical detection device of an embodiment further includes a lateral fluorescence signal collecting assembly 60 for collecting lateral fluorescence generated by the light source 1 irradiating cells passing through the flow chamber 6. In one embodiment, the lateral fluorescence signal collection component 60 includes a fluorescent aperture diaphragm 61, a filter 62, and a photodetector 63 arranged in sequence. The fluorescent aperture diaphragm 61 is used to process lateral fluorescence, and the processed lateral The fluorescence passes through the filter 62 and reaches the photodetector 63, which is used to convert the lateral fluorescence into an electrical signal.

In one embodiment, a collecting lens 7 can be set on the path of the side light of the flow chamber 6 to collect the side light (including side scattered light and side fluorescence), and the dichroic mirror 8 The side scattered light is reflected to the side scattered light signal collection component 50—for example, the dichroic mirror 8 reflects and focuses the side scattered light on the side aperture diaphragm 51, and then enters the photodetector 52; the side fluorescence is transparent Pass the dichroic mirror 8 and enter the lateral fluorescence signal collection component 60-for example, the lateral fluorescence is focused on the fluorescence aperture diaphragm 61 through the dichroic mirror 8 and then reaches the photodetector 63 after passing through the filter 62 .

In the above description of the collection of the side light by the sample optical detection device, the side scattered light and the side fluorescence in an embodiment may be 70 degrees to 110 degrees of light.

It should be noted that FIGS. 10 and 11 are an example in which the sample optical detection device includes both a side scattered light signal collection component 50 and a side fluorescence signal collection component 60. In some embodiments, the sample optical detection device may only It includes one of a side scattered light signal collecting component 50 and a side fluorescent signal collecting component 60.

The above is some explanations that the forward scattered light, side scattered light and side fluorescence generated by the cells passing through the flow chamber 6 from the light source 1 are collected by the corresponding measurement channels. In some embodiments, the light path between the light source 1 and the flow chamber 6 Some devices for improving the optical signal can also be provided. For example, please refer to FIG. 12. In one embodiment, the sample optical detection device may also include a light source shaping component 9 for collimating the light beam emitted by the light source 1 and converging it to the flow Cells of chamber 6. Referring to FIG. 13, in an embodiment, the light source shaping component 9 includes a collimating lens 2, an optical isolator 3, a first cylindrical mirror 4, and a second cylindrical mirror 5 arranged in sequence; the collimating lens 2 is used to align the light source 1 The emitted light beam is collimated, and the optical isolator 3 is used to suppress the feedback light and prevent the reflected light from the subsequent optical devices from entering the light source 1 and affecting the light source 1. The first cylindrical mirror 4 is used to make the light beam pass through the cell Direction converges at the center of the flow chamber, for example, the first cylindrical mirror 4 makes the light beam converge at the center of the flow chamber in the Y-axis direction in the figure; the second cylindrical mirror 5 is used to direct the light beam perpendicular to The cells converge in the passing direction. For example, the second cylindrical mirror 5 is used to converge the light beam in the X-axis direction in the figure, so that all scattered light is irradiated into the straight stop 16. Those skilled in the art can understand that the components in the light source shaping assembly 9 are not necessary in some cases, such as the optical isolator 3 and the second cylindrical mirror 5, etc.

To sum up, the five measurement channels in the sample optical detection device are respectively described. In specific implementation, several of the five measurement channels can be introduced according to requirements. For example, Figure 13 above is a schematic diagram of a sample optical detection device with five measurement channels. Take Figure 13 as an example below to explain the working process and principles of these five measurement channels.

In the figure, the flow direction of the sample cells (that is, the direction through the flow chamber 6) is the Y-axis direction, that is, the direction perpendicular to the paper. The light emitted from the light source 1 passes through the collimator lens 2 to become an equal beam, passes through the optical isolator 3, and then passes through the first cylindrical mirror 4 to make the light beam converge in the direction in which the sample cells flow, that is, in the Y-axis direction. The center of the flow chamber to illuminate the cells passing through the flow chamber 6. The main function of the optical isolator 3 is to suppress the feedback light-the feedback light mainly comes from the reflection of the optical components behind the optical isolator 3-enters the light source 1, preventing the output power of the light source 1 from fluctuating, and ensuring the optical baseline stability.

The second cylindrical lens 5, spherical lenses 14 and 15 will make the beam converge in the X-axis direction. For example, by designing the second cylindrical lens 5, spherical lenses 14 and 15 to make the size of the beam in the X-axis direction Compressed to be smaller than the horizontal size of the straight bar of the straight stop 16 to ensure that the direct light can be effectively blocked by the straight stop 16 to prevent signal saturation; it should be noted that the straight bar of the straight stop 16 is A straight bar along the Y-axis direction, that is, the axial direction of the straight bar is the Y-axis direction, and the lateral direction is the X-axis direction.

When the processed blood cells pass through the flow chamber 6 one by one, the light beam from the light source 1 will finally irradiate the cells passing through the flow chamber 6, producing forward scattered light in the first angular range (the low-angle forward scattered light may be used as an example below. ), the forward scattered light in the second angle range (the forward scattered light at the middle angle may be taken as an example below), the forward scattered light in the third angle range (the forward scattered light at a high angle may be taken as an example below), Side scattered light and side fluorescence:

The low-angle forward scattered light and the medium-angle forward scattered light are collected by spherical lenses 14 and 15, and then the low-angle forward scattered light is reflected by the reflective light blocking component 10 (such as its elliptical aperture mirror) to the first The angle limiting aperture 21 (for example, the low angle limiting aperture) converges at the stray light stop 22, and then enters the photodetector 23; while the forward scattered light of the medium angle is reflected by the light blocking component 10 (for example, it is blocked Strip 12, the shading strip 12 is arranged along the plane direction composed of the X axis and the Z axis, and the effective shielding direction is the X axis direction), which is effectively shielded from the stray light of the forward scattered light at the middle angle, and then converges on the shield The stray light diaphragm 31 then enters the photodetector 32;

The high-angle forward scattered light is reflected by the reflector 41 to the third angular range aperture stop 42 (for example, the high-angle aperture stop), and then enters the photodetector 43;

The side scattered light and the side fluorescence are collected by the collecting lens 7 at the same time, and are spatially separated when passing through the dichroic mirror 8, wherein the side scattered light is reflected and focused on the side small hole through the front surface of the dichroic mirror 8. The diaphragm 51 then enters the photodetector 52; the lateral fluorescence passes through the dichroic mirror 8 and then focuses on the fluorescent aperture diaphragm 61, and then passes through the filter 62 to reach the photodetector 63.

The above are some descriptions of the sample optical detection device of the present invention. The present invention uses lens components to collect low- and mid-angle forward scattered light in forward scattered light, and uses reflective light blocking components to spatially separate low-angle forward scattered light and mid-angle forward scattered light, which not only ensures low-angle The signal quality of forward scattered light and mid-angle forward scattered light, while reducing the longitudinal size of the optical structure, is very conducive to miniaturization; in the compact optical path structure of forward scattered light, the present invention uses the mirror to face the high angle The forward scattered light is collected, which can replace the traditional classification of eosinophils through the side scattered light signal; the present invention adds a medium-angle forward scattered light measurement channel and High-angle forward scattered light measurement channels, through these three measurement channels, some cells can be classified and counted. For example, through these three measurement channels, platelets can be removed from red blood cells without using fluorescent or spheronizing reagents. Classification; the present invention uses a collection lens set on the side light path of the flow chamber to depolarize and collect the fluorescent signal of the side light, and separate the depolarized side scattered light and the fluorescent signal through the wavelength selection of the dichroic mirror . The present invention realizes the optical path structure for receiving scattered light from 0 degree or 1 degree to 110 degrees, and there is no lost angle in the middle.

The above uses specific examples to illustrate the present invention, which are only used to help understand the present invention and not to limit the present invention. For those of ordinary skill in the art, according to the idea of the present invention, the above-mentioned specific embodiments can be changed.

Claims

A sample optical detection device, characterized in that it comprises:

Flow chamber for the cells in the sample to be tested to pass through one by one;

A light source for illuminating the cells passing through the flow chamber;

The first forward scattered light signal collection component is used to collect forward scattered light in a first angular range, and the forward scattered light is the reflected light generated by the light source irradiating cells passing through the flow cell;

The second forward scattered light signal collecting component is used to directly collect forward scattered light in the second angular range, and the forward scattered light is light generated by the light source irradiating cells passing through the flow cell.
The sample optical detection device according to claim 1, further comprising a reflective light blocking component, which is arranged on the optical path of the forward scattered light generated by the cells passing through the flow cell irradiated by the light source, for adjusting the first angle The forward scattered light in the range is reflected to the first forward scattered light signal collection component, and the forward scattered light in the second angular range is allowed to directly enter the second forward scattered light signal collection component.
The sample optical detection device according to claim 2, wherein the reflective light blocking component comprises a mirror for reflecting forward scattered light in the first angular range to the first forward scattered light signal collection Components.
The sample optical inspection device of claim 3, wherein the reflector is elliptical.
The sample optical inspection device according to claim 3 or 4, wherein the reflective light blocking component further comprises a light shielding strip for shielding stray light except for the forward scattered light in the second angular range; wherein The reflecting mirror is arranged on the shading strip.
5. The sample optical inspection device according to claim 5, wherein the reflector is arranged on the light shielding strip in such a way that its long axis coincides with the light shielding strip.
The sample optical detection device according to any one of claims 2 to 6, further comprising a lens assembly, which is arranged on the optical path where the light source irradiates the forward scattered light generated by the cells passing through the flow chamber for The forward scattered light in the first angle range and the second angle range is collected and emitted to the reflective light blocking component.
The sample optical inspection device according to claim 7, wherein the lens assembly includes an aspheric lens and a spherical lens, or multiple aspheric lenses, or multiple spherical lenses, or, an aspheric lens Lens and multiple spherical lenses.
8. The sample optical detection device according to claim 8, wherein when the transparent component includes a spherical lens, the effective data aperture of the spherical lens closest to the flow chamber is at least 0.34.
The sample optical detection device according to claim 7, further comprising a straight blocking diaphragm, which is arranged between the lens assembly and the reflective light blocking assembly, and is used to block the forward scattered light emitted from the lens assembly. The light at a direct angle, and/or the forward scattered light emitted by the lens assembly is limited to the first angle range and the second angle range.
The sample optical detection device according to claim 1, wherein the first forward scattered light signal collection component comprises a first angle limiting diaphragm, a stray light blocking diaphragm, and a photodetector that are sequentially arranged; The first angle limiting diaphragm is used to limit the reflected forward scattered light in a first angular range and converge on the stray light blocking diaphragm, and the stray light blocking diaphragm is used to shield the forward direction of the first angular range Scattered stray light, the photodetector is used to convert the collected forward scattered light in the first angular range into an electrical signal.
The sample optical detection device according to claim 1, wherein the second forward scattered light signal collection component comprises a stray light blocking diaphragm and a photodetector arranged in sequence, and the stray light blocking diaphragm is used for The stray light of the forward scattered light in the second angular range is shielded, and the photodetector is used to convert the collected forward scattered light in the second angular range into an electric signal.
The sample optical detection device of claim 1, further comprising a third forward scattered light signal collection component for collecting forward scattered light in a third angular range, and the forward scattered light is the light source Irradiate the light generated by the cells passing through the flow chamber and reflected at least once.
The sample optical detection device according to claim 13, wherein the third forward scattered light signal collection component comprises a mirror, a third angular range aperture diaphragm, and a photodetector arranged in sequence; the mirror The forward scattered light in the third angular range generated by the light source irradiating the cells passing through the flow chamber is reflected to the third angular range aperture diaphragm, and the third angular range aperture diaphragm is used for forward scattering The light is limited to a third angular range, and the photodetector is used to convert the collected forward scattered light of the third angular range into an electrical signal.
The sample optical detection device according to any one of claims 1 to 14, wherein the first angle range is a low angle range, and/or the second angle range is a medium angle range, and/ Or, the third angle range is a high angle range.
The sample optical inspection device of claim 15, wherein the first angular range and the second angular range are continuous ranges.
The sample optical inspection device according to claim 15, wherein the first angle range, the second angle range and the third angle range are continuous ranges.
The sample optical detection device according to claim 15, wherein the first angle range is 0 to 10 degrees or 1 degree to 10 degrees; and/or, the second angle range is 10 degrees to 20 degrees And/or, the third angle range is 20 degrees to 70 degrees.
5. The sample optical detection device of claim 1, further comprising a light source shaping component for collimating the light beam emitted by the light source and making it converge on the cells passing through the flow chamber.
The sample optical inspection device according to any one of claims 1 to 19, wherein the light source shaping assembly comprises a collimating lens and a first cylindrical lens arranged in sequence, and the collimating lens is used to The light beam emitted by the light source is collimated, and the first cylindrical mirror is used for converging the light beam at the center of the flow chamber in the direction in which the cells pass.
The sample optical detection device according to claim 20, further comprising a second cylindrical mirror arranged on the exit light path of the first cylindrical mirror for directing the light beam in a direction perpendicular to the cell passing direction Convergence, so that all scattered light is irradiated into the straight stop.
22. The sample optical inspection device of claim 20, further comprising an optical isolator disposed between the collimating lens and the first cylindrical mirror to suppress feedback light.
5. The sample optical detection device of claim 1, further comprising:

The side scattered light signal collection component is used to collect the side scattered light generated by the light source irradiating cells passing through the flow chamber; and/or,

The lateral fluorescence signal collection component is used to collect the lateral fluorescence generated by the light source irradiating cells passing through the flow chamber.