CN201984016U - Photoelectric sensor for detecting blue-green algae - Google Patents
Photoelectric sensor for detecting blue-green algae Download PDFInfo
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- CN201984016U CN201984016U CN2010206718319U CN201020671831U CN201984016U CN 201984016 U CN201984016 U CN 201984016U CN 2010206718319 U CN2010206718319 U CN 2010206718319U CN 201020671831 U CN201020671831 U CN 201020671831U CN 201984016 U CN201984016 U CN 201984016U
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Abstract
The utility model relates to a photoelectric sensor for detecting blue-green algae, and a light source and a focusing lens group are arranged in an inner hole of a light source component; a flow chamber is positioned on the right side of a first focusing lens or a lens group, a second focusing lens is arranged in the direction which is vertical to the light beam propagation direction, the second focusing lens is placed in a lens base, a long-pass color filter and a photoelectric detector are placed in a detection component, and the light source component, a flow chamber seat, the lens base and the detection component are connected with a baseplate respectively through threads; a light-emitting diode is adopted as the light source; and a light beam emitted by the light-emitting diode passes through the first focusing lens or the lens group to form an oval light spot which is irradiated to a detection region in the flow chamber. The photoelectric sensor is suitable for field measurement analytical methods of the blue-green algae, and can realize measurement on cells of the blue-green algae, which are contained in a sample to be measured, one by one; not only the concentration of the blue-green algae can be precisely obtained, but also the analysis on different physiological periods of the blue-green algae can be realized, and the early warning on growth status of the blue-green algae in a water area is benefited.
Description
Technical field
The utility model relates to a kind of photoelectric sensor, and especially a kind of water quality composition detects, particularly the photoelectric sensor that blue-green algae detects in the water.
Background technology
The lake is one of human most important water resource, also is China's population and industrial accumulation area on every side in the lake.Because the influence of mankind's activity, this year, the eutrophication in lake was on the rise, and Resource Properties such as Taihu Lake, big-and-middle-sized lake, Chaohu, Dian Chi, Dongting Lake, Hongchehu Lake, Baiyang Lake are on the hazard.The direct result of eutrophication is exactly the generation of blue-green alga bloom, and the blue-green alga bloom of Taihu Lake outburst in 2008 has had a strong impact on peripheral resident's normal life.How before the blue-green alga bloom outburst, to carry out early warning accurately and become extremely important.It is practicable approach that blue-green algae content in the water is carried out continuous monitoring.
Monitoring to blue-green algae all is to judge its concentration by detecting the absorption intensity or the fluorescence intensity of blue-green algae under specific wavelength at present, and its technology is:
1. utilize the phycocyanin in the blue-green algae that the absorption characteristic of the light of specific wavelength is carried out the absorbance log measurement to determine blue-green algae concentration, the quantitative test foundation is a Lambert-Beer's law, we are simply referred to as absorption process, as Fig. 1, comprise light source 1, sample cell 2, optical receiver 3, illumination beam 4, transmitted light beam 5.
2. utilize the fluorescence of phycocyanin specific wavelength of stimulated emission after absorbing light is shone, determine blue-green algae concentration, abbreviate fluorescence method as according to the size of detected fluorescence volume.The excitation wavelength peak value of phycocyanin is at 621nm, and the peak value of emission wavelength is at 646nm, is that the light source with this wave band shines mostly in the prior art.Fig. 2 has provided the synoptic diagram of fluorescence method, comprises light source 1, sample cell 2, illumination beam 6, fluorescent light beam 7, detector 8, collecting lens 9.Fig. 3 is another embodiment of fluorescence method, and ultimate principle is the same, all is the concentration of judging blue-green algae by the size of fluorescence intensity, comprises light source 1, sample cell 2, illumination beam 6, fluorescent light beam 7, detector 8, color filter 10.
The defective of said method is:
1. what detect is the overall of a sample, but not the individuality of each blue-green algae cell, intensity variations amount (absorbing light or fluorescence) can not be directly corresponding with blue-green algae concentration, need realize by the calibration system of complexity, conventional method is a sediments microscope inspection, waste time and energy very much, and same detecting instrument all needs to calibrate again to different waters.
2. because the blue-green algae fluorescence intensity of different physiology phases is different, can cause the inaccurate of concentration calibration.For example: the blue-green algae cell fluorescence amount in growth period is greater than the blue-green algae cell fluorescence amount in maturity stage, so low concentration growth period the blue-green algae cell and the maturity stage blue-green algae cell of high concentration just may obtain same fluorescence volume, this moment, calibration lost meaning.
3. the just concentration by the blue-green algae cell that detects can't judge that the early warning effect is relatively poor to the physiology phase of the blue-green algae in waters.
The best method that addresses the above problem is to adopt flow cytometer that sample to be tested is measured, but flow cytometer is bulky, heavy, cost an arm and a leg, and need outside machine, carry out sample process, only be adapted at lab investigation, can not carry out on-the-spot test and on-line real time monitoring.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, provides a kind of blue-green algae to detect photoelectric sensor, can realize the contained blue-green algae cell of sample to be tested is measured one by one; Not only accurately obtain the concentration of blue-green algae, and can analyze, help the upgrowth situation of blue-green algae in the waters is given warning in advance the different physiology phase of blue-green algae.
The technical scheme that provides according to the utility model, a kind of blue-green algae detects photoelectric sensor and comprises light source, first condenser lens or lens combination, flow chamber, second condenser lens, long logical color filter and photodetector, described light source is positioned at first condenser lens or the lens combination left side, and light source and focus lens group place the endoporus of light source assembly; Flow chamber is placed in the flow chamber seat, and flow chamber is positioned at first condenser lens or lens combination the right; By described flow chamber center, on direction, set gradually second condenser lens, long logical color filter, photodetector perpendicular to beam propagation, long logical color filter and photodetector are placed in the probe assembly, second condenser lens is placed in the lens mount; Described light source assembly, flow chamber seat, lens mount and probe assembly are connected with substrate respectively with screw thread, are characterised in that described light source adopts light emitting diode; The light beam that described light emitting diode sends shines detection zone in the flow chamber through first condenser lens or oval hot spot of lens combination formation, the blue-green algae cell that flows through in the detection zone passes through second condenser lens through the fluorescence that the irradiation back produces, enter photodetector by long logical color filter again and form electric signal, can obtain concentration of blue-green algae and the upgrowth situation of blue-green algae by analysis to these electric signal.
Described flow chamber profile is the guide hole of rectangular parallelepiped, and this guide hole is divided into three zones: commutating zone, accelerating region, detection zone; Described detection zone is that the rayed zone is square hole or the rectangular opening that a length of side is 200um～400um; The part of the inlet flaring of described square hole or rectangular opening forms accelerating region, and the part that sheath fluid passage and sample liquid passage are nested is a commutating zone.
Described sample liquid channel outlet is the circular hole of diameter 0.3mm.
Described light source adopts light emitting diode (LED).
Described photodetector adopts photodiode (PD).
The wavelength of described light source is 620 ± 5nm, and the wavelength of fluorescence that blue-green algae sends is 640nm～850nm.
The utility model has the advantages that:
1. solve the calibration problem of traditional instrument.Blue-green algae cell number contained in the sample to be tested to designated volume is counted one by one, obtains blue-green algae concentration accurately, and need not obtain blue-green algae concentration by the scaling system of complexity, and measurement result is more accurate.
2. when measuring concentration, can analyze, the blue algae growth situation in waters is given warning in advance the different physiology phase of blue-green algae in the sample.
3. with the LED illumination, greatly reduce instrument cost and size, help the field condition operation.
Description of drawings
Fig. 1 is existing transmission beam method schematic diagram.
Fig. 2 is existing fluorescence method schematic diagram.
Fig. 3 is a blue-green algae fluorescence detecting sensor schematic diagram of using optical fiber.
Fig. 4 is the systematic schematic diagram that adopts the utility model to detect.
Fig. 5 is the utility model photoelectric sensor use principle figure.
Fig. 6 (a) is the flow chamber sectional view.
Fig. 6 (b) is the A-A sectional view of Fig. 6 (a).
Fig. 7 is the detection synoptic diagram of detection zone in the flow chamber.
Fig. 8 is the utility model one-piece construction schematic perspective view.
Embodiment
The utility model is described in further detail below in conjunction with drawings and Examples.
As Fig. 4～shown in Figure 8: comprise light source 1, flow chamber 12, first condenser lens or lens combination 16, second condenser lens 7, long logical color filter 10, photodetector 8, substrate 20, probe assembly 60, lens mount 50, flow chamber seat 40, light source assembly 30, signal Processing and data analysis unit 200 and suction sample and liquid road control module 300.
Described light irradiation unit 11 comprises light source 1, first condenser lens or lens combination 16, forms a focal beam spot and shines on the sample flow that flows through in the flow chamber; Described light irradiation unit comprises a red LED at least as light source, and its wavelength is at 620nm ± 5nm.Light irradiation unit is placed in the light source assembly 30.
Described flow chamber 12 is made by optically transparent material, in open a guide hole, two kinds of flow of liquid mistakes are arranged in guide hole, a kind of is the sample to be tested liquid that contains the blue-green algae cell, another is a sheath fluid, sample liquid passes through blue-green algae cell contained in the sample liquid in the dirty via flow of the parcel of sheath fluid chamber singly.Flow chamber 12 usefulness glue are connected in the flow chamber seat 40.
Described light-receiving probe unit 13 comprises second condenser lens 7, long logical color filter 10, photodetector 8.The light signal that described light-receiving probe unit 13 produces after the particle in the sample flow is subjected to shining is collected on the detector, and carries out opto-electronic conversion.Wherein second condenser lens 7 is arranged in lens mount 50, and long logical color filter 10 and photodetector 8 are arranged in probe assembly 60.
As Fig. 5, shown in Figure 8: suppose that the light that light source sends propagates from left to right, described light source 1 is positioned at first condenser lens or lens combination 16 left sides, and light source 1 and focus lens group 16 place the endoporus of light source assembly 30; Flow chamber 12 is placed in the flow chamber seat 40, and flow chamber 12 is positioned at first condenser lens or lens combination 16 the right; Described flow chamber 12 belows, second condenser lens 7 is set on the direction perpendicular to beam propagation, second condenser lens 7 is placed in the lens mount 50, described condenser lens 7 belows set gradually long logical color filter 10, photodetector 8, long logical color filter 10 and photodetector 8 are placed in the probe assembly 60, and photodetector 8 is connected with data analysis unit 200 with signal Processing; Described light source assembly 30, flow chamber seat 40, lens mount 50 and probe assembly 60 usefulness screw threads are connected with substrate 20.The right-hand direction of beam propagation that is meant as herein described, below are the directions that direction of beam propagation clockwise rotates 90 degree.
Described light source 1 light beam shines detection zones 123 in the flow chamber 12 through first condenser lens or oval hot spot 14 of lens combination 16 formation, and the fluorescence of described flow chamber 12 enters photodetector 8 by long logical color filter 10 again by second condenser lens 7.
As Fig. 6 (a), shown in Fig. 6 (b): as described in flow chamber 12 be that the profile that an optically transparent material is made is rectangular parallelepiped or other cubical guide holes, this guide hole is divided into three zones: commutating zone 121, accelerating region 122, detection zone 123.Wherein detection zone 123, and just the rayed zone is square hole or the rectangular opening (this paper is called for short square hole) that a length of side is 200um～400um, as Fig. 6 (a) (b) shown in.The inlet flaring of described square hole is the sheath fluid passage, parallel insertion sample liquid passage in the sheath fluid passage, sample liquid channel outlet is in described square hole inlet flaring zone, with the square hole inlet over against, described sample liquid channel outlet is the circular hole of diameter 0.3mm.The part of the inlet flaring of square hole forms accelerating region 122, and the part that sheath fluid passage and sample liquid passage are nested is a commutating zone 121.The sheath fluid stream of flow chamber 12 internal flows will satisfy laminar flow condition, i.e. Reynolds number＜2300.Sheath stream carries out fluid focus to sample flow, and sample flow is compressed to width less than 2 blue-green algae cells, so that blue-green algae cell passing through one by one makes each detection incident only detect a cell.Sheath fluid forms laminar flow in commutating zone 121, in accelerating region 122, sample liquid is focused on gradually, until the sample flow that in detection zone sample hydraulic pressure is shortened into about 20um, the blue-green algae cell 15 in the sample liquid just can only pass through the irradiation of detection zone irradiates light one by one like this, as shown in Figure 7.
In order to reduce cost and to reduce size, make system can be applicable to on-the-spot the detection, the utility model adopts LED as light source 1, and the LED wavelength is 620 ± 5nm.The fluorescence excitation spectrum of phycocyanin is near 620nm, the light beam that LED sends is through first condenser lens (group) 16, forming one is about 20um at cell flow direction (shown in the arrow), perpendicular to cell flow and the direction of beam propagation on for the oval hot spot 14 about 200um shines on the cell, as shown in Figure 7.Focal beam spot in the size requirements of flow chamber center is: less than 2 blue-green algae diameters, be not less than hole width in the flow chamber on the direction that flows perpendicular to beam propagation and sample at the same time on the sample flow direction.On perpendicular to the direction of beam propagation with second condenser lens 7 of a large-numerical aperture with phosphor collection to photodetector 8, what photodetector 8 of the present utility model adopted is photoelectric diode.In order to eliminate bias light, obtain good signal-to-noise, before photodetector 8, increase a long logical color filter 10, bias light (mainly being irradiates light) is suppressed to greatest extent, allow fluorescence pass through simultaneously.
Shown in Figure 4: the utility model and signal Processing and data analysis unit 200, suction sample and liquid road control module 300 are formed the system of blue-green algae cell detection.Light-receiving probe unit 13 output terminals of described photoelectric sensor 100 are connected with data analysis unit 200 input ends with signal Processing, and described suction sample is connected with the flow chamber 12 of photoelectric sensor 100 with liquid road control module 300.
Blue-green algae cell 15 is by the irradiation of detection zone irradiates light, through producing fluorescence after the irradiation, fluorescence signal is received by photodetection unit 13, form electric signal through after the opto-electronic conversion, detection zone is whenever by a blue-green algae cell, electric impulse signal of photodetection unit 13 outputs is the fluorescence intensity correspondence of cell therewith, these electric impulse signals are sent to signal Processing and data analysis unit 200, the fluorescence signal of signal Processing and 200 pairs of blue-green algaes of data analysis unit carries out Filtering Processing, then the statistical information of sample is analyzed.The corresponding pulse of blue-green algae cell, the amplitude of pulse has reflected the fluorescence volume size of cell, has so just realized the detection of individual cells in the sample.Detect up to ten thousand blue-green algae cells, forming statistical property is exactly the general characteristic of this sample: the total number of pulse is exactly the blue-green algae concentration of sample divided by tested volume, and the amplitude of all pulses in the sample is carried out the growth and development state that statistical study can obtain this sample blue-green algae.
Utilize the utility model, not only can carry out quantitative measurment, further, can carry out analysis and judgement, in order to judge the upgrowth situation of waters, tested sample place blue-green algae the different physiology phase of blue-green algae to the concentration of blue-green algae in the waters.
Claims (5)
1. a blue-green algae detects photoelectric sensor, comprise light source (1), first condenser lens or lens combination (16), flow chamber (12), second condenser lens (7), long logical color filter (10) and photodetector (8), described light source (1) is positioned at first condenser lens or lens combination (16) left side, and light source (1) and focus lens group (16) place the endoporus of light source assembly (30); Flow chamber (12) is placed in the flow chamber seat (40), and flow chamber (12) is positioned at first condenser lens or lens combination (16) the right; By described flow chamber (12) center, on direction, set gradually second condenser lens (7), long logical color filter (10), photodetector (8) perpendicular to beam propagation, long logical color filter (10) and photodetector (8) are placed in the probe assembly (60), and second condenser lens (7) is placed in the lens mount (50); Described light source assembly (30), flow chamber seat (40), lens mount (50) and probe assembly (60) are connected with substrate (20) respectively with screw thread, it is characterized in that described light source (1) adopts light emitting diode; The light beam that described light emitting diode sends shines the interior detection zone of flow chamber (12) (123) through first condenser lens or lens combination (a 16) oval hot spot of formation (14).
2. a kind of according to claim 1 blue-green algae detects photoelectric sensor, and it is characterized in that: described flow chamber (12) profile is the guide hole of rectangular parallelepiped, and this guide hole is divided into three zones: commutating zone (121), accelerating region (122), detection zone (123); Described detection zone (123) is that the rayed zone is square hole or the rectangular opening that a length of side is 200um～400um; The part of the inlet flaring of described square hole or rectangular opening forms accelerating region (122), and the part that sheath fluid passage and sample liquid passage are nested is commutating zone (121).
3. detect photoelectric sensor as a kind of blue-green algae as described in the claim 2, it is characterized in that: described sample liquid channel outlet is the circular hole of diameter 0.3mm.
4. a kind of according to claim 1 blue-green algae detects photoelectric sensor, it is characterized in that: described photodetector (8) adopts photodiode.
5. a kind of according to claim 1 blue-green algae detects photoelectric sensor, and it is characterized in that: the wavelength of described light source (1) is 620 ± 5nm, and the printing opacity wavelength is 640nm～850nm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010206718319U CN201984016U (en) | 2010-12-21 | 2010-12-21 | Photoelectric sensor for detecting blue-green algae |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010206718319U CN201984016U (en) | 2010-12-21 | 2010-12-21 | Photoelectric sensor for detecting blue-green algae |
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| CN201984016U true CN201984016U (en) | 2011-09-21 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507418A (en) * | 2011-10-26 | 2012-06-20 | 长春迪瑞医疗科技股份有限公司 | Particle imaging chamber |
| GB2504981A (en) * | 2012-08-16 | 2014-02-19 | Natural Enviromental Res Council | Sensing apparatus and method for measuring algal growth |
| CN109891212A (en) * | 2016-10-24 | 2019-06-14 | 皇家飞利浦有限公司 | Optical particle detectors |
-
2010
- 2010-12-21 CN CN2010206718319U patent/CN201984016U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507418A (en) * | 2011-10-26 | 2012-06-20 | 长春迪瑞医疗科技股份有限公司 | Particle imaging chamber |
| GB2504981A (en) * | 2012-08-16 | 2014-02-19 | Natural Enviromental Res Council | Sensing apparatus and method for measuring algal growth |
| CN109891212A (en) * | 2016-10-24 | 2019-06-14 | 皇家飞利浦有限公司 | Optical particle detectors |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110921 Termination date: 20121221 |