ZA964057B - Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers. - Google Patents

Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers.

Info

Publication number
ZA964057B
ZA964057B ZA9604057A ZA964057A ZA964057B ZA 964057 B ZA964057 B ZA 964057B ZA 9604057 A ZA9604057 A ZA 9604057A ZA 964057 A ZA964057 A ZA 964057A ZA 964057 B ZA964057 B ZA 964057B
Authority
ZA
South Africa
Prior art keywords
preamplification
exonucleases
sterilization
methods
chain reaction
Prior art date
Application number
ZA9604057A
Other languages
English (en)
Inventor
John Wesley Backus
David Robert Patterson
John William Hender Sutherland
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Publication of ZA964057B publication Critical patent/ZA964057B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/149—Concentration of an enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
ZA9604057A 1995-05-22 1996-05-21 Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers. ZA964057B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44700095A 1995-05-22 1995-05-22

Publications (1)

Publication Number Publication Date
ZA964057B true ZA964057B (en) 1997-11-21

Family

ID=23774610

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA9604057A ZA964057B (en) 1995-05-22 1996-05-21 Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers.

Country Status (9)

Country Link
EP (1) EP0744470A1 (xx)
JP (1) JPH09107997A (xx)
KR (1) KR960041377A (xx)
CN (1) CN1140762A (xx)
AU (1) AU5233996A (xx)
CA (1) CA2176193A1 (xx)
IL (1) IL118227A0 (xx)
NO (1) NO962062L (xx)
ZA (1) ZA964057B (xx)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
US6274353B1 (en) 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
EP1088891B1 (en) 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro
DE10061166A1 (de) * 2000-11-30 2002-06-06 Attomol Gmbh Molekulare Diagno Verfahren und Kit zum direkten Nachweis von Nukleotidsequenzen, Aminosäuresequenzen oder Antigenen
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US8361712B2 (en) 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
EP1277841B1 (en) * 2001-07-11 2009-08-26 Roche Diagnostics GmbH New composition and method for hot start nucleic acid amplification
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
CA2409775C (en) * 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
EP1327682B1 (de) * 2002-01-11 2009-05-13 BioSpring Gesellschaft für Biotechnologie mbH Verfahren zur Herstellung von DNA
US20030190626A1 (en) * 2002-04-09 2003-10-09 Vasulinga Ravikumar Phosphorothioate monoester modified oligomers
WO2004003228A1 (en) * 2002-07-01 2004-01-08 Unisearch Limited Genotyping method
AU2003257013A1 (en) * 2003-07-29 2005-03-07 Sigma Aldrich Co. Methods and compositions for amplification of dna
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
EP1502961B1 (en) * 2003-08-01 2010-09-08 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
DE102005047617A1 (de) * 2005-10-05 2007-04-19 Qiagen Gmbh Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft
ES2364983T3 (es) 2006-02-27 2011-09-20 F. Hoffmann-La Roche Ag Reacción en cadena de polimerasa con arranque en caliente por secuestro de magnesio.
US20130323795A1 (en) 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
US8586310B2 (en) * 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US9909170B2 (en) 2008-09-05 2018-03-06 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US11111514B2 (en) 2008-09-05 2021-09-07 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
PL2828399T3 (pl) * 2012-03-22 2017-07-31 Lgc Genomics Limited Układ wykrywający do reakcji łańcuchowej polimerazy z użyciem oligonukleotydów zawierających grupę tiofosforanową
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
GB2515990B (en) 2013-03-06 2016-05-04 Lgc Genomics Ltd Polymerase chain reaction detection system
JP6786390B2 (ja) * 2013-08-13 2020-11-25 ゼネラル・エレクトリック・カンパニイ コンタミネーションフリー試薬を使用したエンドヌクレアーゼ支援等温増幅
WO2016144810A1 (en) * 2015-03-06 2016-09-15 Sigma-Aldrich Co. Llc Nucleic acid amplification and library preparation
US11396674B2 (en) * 2019-02-22 2022-07-26 Gianluca ROMA Multiplex nucleic acid amplification and library preparation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2663949A1 (fr) * 1990-06-29 1992-01-03 Genset Sa Perfectionnements a un procede d'amplification enzymatique in vitro.
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
CA2101119A1 (en) * 1992-08-17 1994-02-18 George T. Walker Exonuclease decontamination method
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody

Also Published As

Publication number Publication date
AU5233996A (en) 1996-12-05
EP0744470A1 (en) 1996-11-27
JPH09107997A (ja) 1997-04-28
NO962062D0 (no) 1996-05-21
CN1140762A (zh) 1997-01-22
IL118227A0 (en) 1996-09-12
KR960041377A (ko) 1996-12-19
NO962062L (no) 1996-11-25
CA2176193A1 (en) 1996-11-23

Similar Documents

Publication Publication Date Title
ZA964057B (en) Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers.
AU4322989A (en) Methods for generating single stranded dna by the polymerase chain reaction
AU4332399A (en) Rna polymerase chain reaction
EP0870832A4 (en) NEW DNA POLYMERASE
CA2153898A1 (en) Method for generating single-stranded dna molecules
EP0605003A3 (en) Test procedure for the detection of the presence of bacteria.
HUP9802024A3 (en) Altered thermostable dna polymerases for sequencing
AU2277195A (en) Purified dna polymerase from bacillus stearothermophilus
IL127142A0 (en) Immune cells having predefined biological specificity
EP0377832A3 (en) Process for the treatment of biomasses, e.g. sewage sludge, manure, etc.
AU6123899A (en) Supports for immobilizing dna or the like
EP0656070A4 (en) NUCLEIC ACID SAMPLES FOR LEGIONELLA GENERATIONS.
AU675251B2 (en) Flow-through hybridization assay for oligonucleotide sequences
HUP0204007A3 (en) Method for biological effluent treatment
AU4897896A (en) Primers for the pcr amplification of metastatic sequences
LTIP1519A (en) Solution, set, process for elongation of the oligonucleotidic primer, process of dna amplification
EP0438292A3 (en) Method of preparing primers for polymerase chain reaction and dna amplification using said primers
IL129165A0 (en) Thermostable DNA polymerase from anaerocellum thermophilum
WO2001040521A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
AU7027500A (en) Chimeric thermostable dna polymerases
AU1426392A (en) 5' and 3' polymerase chain reaction walking from known dna sequences
HK1043157A1 (zh) 使用聚合酶鏈反應測定念珠菌
AU1774301A (en) Methods for treating wastewater sludge
WO2000029622A3 (en) Nucleic acids containing single nucleotide polymorphisms and methods of use thereof
AU1030500A (en) Method for the treatment of sewage sludge, liquid manure or the like by electrofloatation