IL118227A0 - Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers - Google Patents
Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primersInfo
- Publication number
- IL118227A0 IL118227A0 IL11822796A IL11822796A IL118227A0 IL 118227 A0 IL118227 A0 IL 118227A0 IL 11822796 A IL11822796 A IL 11822796A IL 11822796 A IL11822796 A IL 11822796A IL 118227 A0 IL118227 A0 IL 118227A0
- Authority
- IL
- Israel
- Prior art keywords
- preamplification
- exonucleases
- sterilization
- methods
- chain reaction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/319—Exonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/149—Concentration of an enzyme
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US44700095A | 1995-05-22 | 1995-05-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL118227A0 true IL118227A0 (en) | 1996-09-12 |
Family
ID=23774610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL11822796A IL118227A0 (en) | 1995-05-22 | 1996-05-12 | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0744470A1 (xx) |
| JP (1) | JPH09107997A (xx) |
| KR (1) | KR960041377A (xx) |
| CN (1) | CN1140762A (xx) |
| AU (1) | AU5233996A (xx) |
| CA (1) | CA2176193A1 (xx) |
| IL (1) | IL118227A0 (xx) |
| NO (1) | NO962062L (xx) |
| ZA (1) | ZA964057B (xx) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6482590B1 (en) * | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| WO1999034014A2 (en) * | 1997-12-23 | 1999-07-08 | Roche Diagnostics Gmbh | A method for the determination of a nucleic acid |
| US6274353B1 (en) | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| EP1088891B1 (en) * | 1999-09-28 | 2005-01-12 | Roche Diagnostics GmbH | Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro |
| DE10061166A1 (de) * | 2000-11-30 | 2002-06-06 | Attomol Gmbh Molekulare Diagno | Verfahren und Kit zum direkten Nachweis von Nukleotidsequenzen, Aminosäuresequenzen oder Antigenen |
| US8507662B2 (en) | 2001-01-19 | 2013-08-13 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| US8361712B2 (en) | 2007-12-17 | 2013-01-29 | General Electric Company | Contamination-free reagents for nucleic acid amplification |
| EP1275735A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
| EP1277841B1 (en) * | 2001-07-11 | 2009-08-26 | Roche Diagnostics GmbH | New composition and method for hot start nucleic acid amplification |
| CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| ATE431407T1 (de) * | 2002-01-11 | 2009-05-15 | Biospring Ges Fuer Biotechnolo | Verfahren zur herstellung von dna |
| US20030190626A1 (en) * | 2002-04-09 | 2003-10-09 | Vasulinga Ravikumar | Phosphorothioate monoester modified oligomers |
| WO2004003228A1 (en) * | 2002-07-01 | 2004-01-08 | Unisearch Limited | Genotyping method |
| WO2005017173A1 (en) * | 2003-07-29 | 2005-02-24 | Sigma Aldrich Co. | Methods and compositions for amplification of dna |
| EP1502958A1 (en) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | New detection format for hot start real time polymerase chain reaction |
| EP1502961B1 (en) * | 2003-08-01 | 2010-09-08 | Roche Diagnostics GmbH | New detection format for hot start real time polymerase chain reaction |
| DE102005047617A1 (de) * | 2005-10-05 | 2007-04-19 | Qiagen Gmbh | Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft |
| ATE510027T1 (de) | 2006-02-27 | 2011-06-15 | Hoffmann La Roche | Pcr-heissstart durch magnesium-sequestrierung |
| US20130323795A1 (en) | 2007-01-10 | 2013-12-05 | General Electric Company | Endonuclase-assisted isothermal amplification using contamination-free reagents |
| CA2658520C (en) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Nucleic acid amplification in the presence of modified randomers |
| US9909170B2 (en) | 2008-09-05 | 2018-03-06 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US8586310B2 (en) * | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US11111514B2 (en) | 2008-09-05 | 2021-09-07 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| US7910720B2 (en) | 2008-09-09 | 2011-03-22 | Roche Diagnostics Operations, Inc. | Polyanion for improved nucleic acid amplification |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| US9133509B2 (en) | 2012-03-22 | 2015-09-15 | Lgc Genomics Limited | Polymerase chain reaction detection system |
| RS55868B1 (sr) * | 2012-03-22 | 2017-08-31 | Lgc Genomics Ltd | Sistem za detekciju lančane reakcije polimeraze pomoću oligonukleotida koji sadrže fosforotioatnu grupu |
| GB2515990B (en) | 2013-03-06 | 2016-05-04 | Lgc Genomics Ltd | Polymerase chain reaction detection system |
| WO2015022359A1 (en) * | 2013-08-13 | 2015-02-19 | General Electric Company | Endonuclease-assisted isothermal amplification using contamination-free reagents |
| US10619199B2 (en) * | 2015-03-06 | 2020-04-14 | Sigma-Aldrich Co. Llc | Nucleic acid amplification and library preparation |
| US11396674B2 (en) * | 2019-02-22 | 2022-07-26 | Gianluca ROMA | Multiplex nucleic acid amplification and library preparation |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2663949A1 (fr) * | 1990-06-29 | 1992-01-03 | Genset Sa | Perfectionnements a un procede d'amplification enzymatique in vitro. |
| US5518900A (en) * | 1993-01-15 | 1996-05-21 | Molecular Tool, Inc. | Method for generating single-stranded DNA molecules |
| CA2101119A1 (en) * | 1992-08-17 | 1994-02-18 | George T. Walker | Exonuclease decontamination method |
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
-
1996
- 1996-05-09 CA CA002176193A patent/CA2176193A1/en not_active Abandoned
- 1996-05-12 IL IL11822796A patent/IL118227A0/xx unknown
- 1996-05-16 AU AU52339/96A patent/AU5233996A/en not_active Abandoned
- 1996-05-21 NO NO962062A patent/NO962062L/no unknown
- 1996-05-21 ZA ZA9604057A patent/ZA964057B/xx unknown
- 1996-05-21 KR KR1019960017155A patent/KR960041377A/ko not_active Application Discontinuation
- 1996-05-21 EP EP96303584A patent/EP0744470A1/en not_active Withdrawn
- 1996-05-21 JP JP8125929A patent/JPH09107997A/ja active Pending
- 1996-05-22 CN CN96110078A patent/CN1140762A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ZA964057B (en) | 1997-11-21 |
| JPH09107997A (ja) | 1997-04-28 |
| CN1140762A (zh) | 1997-01-22 |
| AU5233996A (en) | 1996-12-05 |
| EP0744470A1 (en) | 1996-11-27 |
| NO962062L (no) | 1996-11-25 |
| KR960041377A (ko) | 1996-12-19 |
| CA2176193A1 (en) | 1996-11-23 |
| NO962062D0 (no) | 1996-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL118227A0 (en) | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers | |
| AU4322989A (en) | Methods for generating single stranded dna by the polymerase chain reaction | |
| EP0870832A4 (en) | NEW DNA POLYMERASE | |
| AU4332399A (en) | Rna polymerase chain reaction | |
| AU4337089A (en) | Structure-independent dna amplification by the polymerase chain reaction | |
| EP0521108A4 (en) | Site specific cleavage of single-stranded dna | |
| HUP9802024A3 (en) | Altered thermostable dna polymerases for sequencing | |
| AU2277195A (en) | Purified dna polymerase from bacillus stearothermophilus | |
| HUP0103214A3 (en) | Method of dna vaccination | |
| AU2786799A (en) | Sonic method of enhancing chemical reactions | |
| HUP0003163A3 (en) | Treating sewage or like sludge | |
| AU675251B2 (en) | Flow-through hybridization assay for oligonucleotide sequences | |
| HUP0204007A3 (en) | Method for biological effluent treatment | |
| AU4897896A (en) | Primers for the pcr amplification of metastatic sequences | |
| GB9007137D0 (en) | Selective inhibition of dna polymerase | |
| EP0438292A3 (en) | Method of preparing primers for polymerase chain reaction and dna amplification using said primers | |
| AU6366100A (en) | Novel methods and reagents for the treatment of osteoarthritis | |
| GB9526218D0 (en) | DNA Sequences | |
| AU1426392A (en) | 5' and 3' polymerase chain reaction walking from known dna sequences | |
| HUP0000640A2 (en) | Process and device for the biological purification of sewage | |
| KR100245951B1 (en) | Process for the selection of tem-like beta-lactamase-producing strains by using of polymerase chain reaction | |
| EP0774517A4 (en) | METHOD FOR INDIVIDUAL DETECTION BY POLYMERASE CHAIN REACTION WITH MI-PRIMER | |
| AU6006196A (en) | Materials and methods for the polymerase chain reaction | |
| GB9719359D0 (en) | The Use of DNA Sequences | |
| WO1999051531A8 (en) | Waste treatment system and method |