ZA865787B - An alkaline protease a process for the preparation of hybrid vectors and genetically transformed microorganisms - Google Patents

An alkaline protease a process for the preparation of hybrid vectors and genetically transformed microorganisms

Info

Publication number
ZA865787B
ZA865787B ZA865787A ZA865787A ZA865787B ZA 865787 B ZA865787 B ZA 865787B ZA 865787 A ZA865787 A ZA 865787A ZA 865787 A ZA865787 A ZA 865787A ZA 865787 B ZA865787 B ZA 865787B
Authority
ZA
South Africa
Prior art keywords
preparation
alkaline protease
genetically transformed
hybrid vectors
transformed microorganisms
Prior art date
Application number
ZA865787A
Inventor
Harald Berger
Friedhelm Bartnik
Werner Goebel
Juergen Kreft
Original Assignee
Henkel Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6277629&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ZA865787(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Henkel Kgaa filed Critical Henkel Kgaa
Publication of ZA865787B publication Critical patent/ZA865787B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A structural gene which codes for the protease subtilisin Carlsberg or its fragments with proteolytic activity or variants with proteolytic activity, including leader sequences thereof, is described. Also described are hybrid plasmids and microorganism strains which contain this structural gene, and a process for separating these microorganism strains from inactive strains. <IMAGE>
ZA865787A 1985-08-03 1986-08-01 An alkaline protease a process for the preparation of hybrid vectors and genetically transformed microorganisms ZA865787B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853527913 DE3527913A1 (en) 1985-08-03 1985-08-03 ALKALINE PROTEASE, METHOD FOR PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS

Publications (1)

Publication Number Publication Date
ZA865787B true ZA865787B (en) 1987-03-25

Family

ID=6277629

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA865787A ZA865787B (en) 1985-08-03 1986-08-01 An alkaline protease a process for the preparation of hybrid vectors and genetically transformed microorganisms

Country Status (7)

Country Link
EP (1) EP0214435B1 (en)
JP (1) JPS6232888A (en)
AT (1) ATE78868T1 (en)
AU (1) AU604640B2 (en)
DE (2) DE3527913A1 (en)
DK (1) DK362686A (en)
ZA (1) ZA865787B (en)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK564086A (en) * 1986-11-25 1988-06-17 Novo Industri As ENZYMATIC DETERGENT ADDITIVE
WO1988006624A2 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Molecular cloning and expression of genes encoding proteolytic enzymes
JPH0787790B2 (en) * 1987-03-04 1995-09-27 三菱瓦斯化学株式会社 METHOD FOR PRODUCING NOVEL GENE DNA AND ALKALINE PROTEASE
JPH0783716B2 (en) * 1987-11-27 1995-09-13 昭和電工株式会社 Gene system encoding alkaline protease
DE3821491A1 (en) * 1988-06-25 1989-12-28 Henkel Kgaa HYBRID PLASMIDE FOR THE PRODUCTION OF SUBTILISIN CARLSBERG IN BACILLUS
DE3824827A1 (en) * 1988-07-21 1990-02-01 Henkel Kgaa PLASMIDES FOR GENERATING ALPHA AMYLASE IN BACILLUS
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
EP0493398B1 (en) * 1989-08-25 1999-12-08 Henkel Research Corporation Alkaline proteolytic enzyme and method of production
WO1991002803A1 (en) * 1989-08-25 1991-03-07 Henkel Research Corporation A method of suppressing chromosomal gene expression
DE3928046A1 (en) * 1989-08-25 1991-02-28 Henkel Kgaa HYBRID PLASMIDE FOR THE PRODUCTION OF SUBTILISIN CARLSBERG IN BACILLUS
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
US5275945A (en) * 1991-10-08 1994-01-04 Vista Chemical Company Alkaline proteases stable in heavy-duty detergent liquids
DE4231726A1 (en) * 1992-09-23 1994-03-24 Cognis Bio Umwelt Mutated subtilisin-like serine proteases
KR100561826B1 (en) 1996-11-04 2006-03-16 노보자임스 에이/에스 Subtilase variants and compositions
DE69739020D1 (en) 1996-11-04 2008-11-13 Novozymes As SUBTILASE VARIANTS AND COMPOUNDS
EP1009815B1 (en) 1997-08-29 2008-01-30 Novozymes A/S Protease variants and compositions
WO1999011769A1 (en) 1997-08-29 1999-03-11 Novo Nordisk A/S Protease variants and compositions
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
BR9814236A (en) 1997-11-21 2000-10-03 Novo Nordisk As Subtilase enzyme, its variant, isolated DNA sequence, expression vector, host microbial cell, process to produce a subtilase or a subtilase variant, composition, and use of a subtilase or a subtilase variant.
ATE504651T1 (en) 1998-12-18 2011-04-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AN ADDITIONAL AMINO ACID RESIDUE IN AN ACTIVE LOOP REGION
WO2000071685A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 132 and 133
ATE407201T1 (en) 1999-05-20 2008-09-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESIDUE BETWEEN POSITIONS 126 AND 127
EP1183342B2 (en) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
DE60039693D1 (en) 1999-05-20 2008-09-11 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESISTANCE BETWEEN POSITIONS 125 AND 126
EP1183341B2 (en) 1999-05-20 2012-05-02 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128
AU4392700A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 129 and 130
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US7507569B2 (en) 2003-05-07 2009-03-24 Novozymes A/S Variant subtilisin enzymes (subtilases)
JP4880469B2 (en) 2003-10-23 2012-02-22 ノボザイムス アクティーゼルスカブ Protease with improved stability in detergents
US20100029538A1 (en) 2006-04-14 2010-02-04 Anna-Liisa Auterinen One-Step Treatment of Textiles
JP2009534022A (en) 2006-04-20 2009-09-24 ノボザイムス アクティーゼルスカブ A sabinase variant with improved detergency against egg stains
KR101522042B1 (en) 2007-04-30 2015-05-20 다니스코 유에스 인크. Use of protein hydrolysates to stabilize metalloprotease detergent formulations
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
EP2606146A1 (en) 2010-08-19 2013-06-26 Novozymes A/S Induced sporulation screening method
GB201212932D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
GB201212934D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
CN116322625A (en) 2020-08-24 2023-06-23 诺维信公司 Oral care compositions comprising levanase

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
HU182981B (en) * 1978-07-04 1984-03-28 Novo Industri As Process for producing protease consentrate
DE2925427C2 (en) * 1979-06-23 1983-12-22 Henkel KGaA, 4000 Düsseldorf Process for the production of protease
DE3380878D1 (en) * 1982-11-01 1989-12-28 Miles Inc Method of heterologous cloning of gene in bacillus microorganism
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
JPS6030685A (en) * 1983-07-29 1985-02-16 Lion Corp Recombinant dna, its preparation, bacillus subtilis containing the same, and preparation of protease using said bacillus subtilis
JPS6112287A (en) * 1984-06-26 1986-01-20 Lion Corp Recombinant dna, its preparation, bacterial strain containing same, preparation of exocytic secretion enzyme using same, and dna for promoting secretion of exocytic enzyme
US4617265A (en) * 1984-09-19 1986-10-14 Board Of Regents, University Of Texas System Colony blot assay for enterotoxigenic bacteria

Also Published As

Publication number Publication date
DE3527913A1 (en) 1987-02-12
AU604640B2 (en) 1991-01-03
DK362686D0 (en) 1986-07-30
ATE78868T1 (en) 1992-08-15
DE3686217D1 (en) 1992-09-03
EP0214435B1 (en) 1992-07-29
JPS6232888A (en) 1987-02-12
AU6078986A (en) 1987-02-05
DK362686A (en) 1987-02-04
EP0214435A2 (en) 1987-03-18
EP0214435A3 (en) 1990-01-17

Similar Documents

Publication Publication Date Title
ZA865787B (en) An alkaline protease a process for the preparation of hybrid vectors and genetically transformed microorganisms
GR3032790T3 (en) Materials and methods for hypersecretion of amino acids
BG49049A3 (en) Method for clonalling and exposure of genetics coding enzyms
AU3015489A (en) Process for integration of a chosen gene on the chromosome of a bacterium and bacterium obtained by the said process
ATE123520T1 (en) KEX2 ENDOPROTEASE AND METHOD FOR THE PRODUCTION THEREOF.
AU5910086A (en) Bacterial enzymes
AU7505796A (en) Hyperthermostable protease gene
CA2093950A1 (en) Subtilisin-like protease from aspergillus niger
DE3666838D1 (en) Plasmid vectors for expression in escherichia coli and/or bacillus subtilis
EP0219034A3 (en) Process for the preparation of mesophilic microorganisms containing d-hydantoinase active at a higher temperature
EP0409521A3 (en) Improved enzymatic oxidation of ceph c to glutaryl-7-amino-cephalosporanic acid
DK0396436T3 (en) Yeast strains for the production of mature heterologous proteins, especially hirudin
EP0092235A3 (en) Amylase-negative, asporogenous mutant of bacillus subtilis useful as a host in a host-vector system
GB2166743B (en) Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression
GR3002696T3 (en) Process for production of isulin-like growth factor i and plasmid for production thereof
IT8423190A1 (en) PROCEDURE FOR THE PRODUCTION OF NEUTRAL PROTEASE
ZA936507B (en) Process for the production of protease inhibitors
EP0121569A4 (en) Novel vector.
ATE66250T1 (en) EXPRESSION PLASMIDS USABLE IN BACILLUS SUBTILIS.
DK251385A (en) CULTURE OF BACILLUS SUBTILIS MUTANT AND PROCEDURES FOR PRODUCING THEREFORE AND PROCEDURES FOR USING IT
IE872279L (en) Crystalline n-(s-3-methylheptanoyl)-d-gamma-glutamyl-glycyl¹-d-alanine.