AU6078986A - An alkaline protease, a process for the preparation of hybrid vectors and genetically transformed microorganisms - Google Patents
An alkaline protease, a process for the preparation of hybrid vectors and genetically transformed microorganismsInfo
- Publication number
- AU6078986A AU6078986A AU60789/86A AU6078986A AU6078986A AU 6078986 A AU6078986 A AU 6078986A AU 60789/86 A AU60789/86 A AU 60789/86A AU 6078986 A AU6078986 A AU 6078986A AU 6078986 A AU6078986 A AU 6078986A
- Authority
- AU
- Australia
- Prior art keywords
- preparation
- alkaline protease
- genetically transformed
- hybrid vectors
- transformed microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A structural gene which codes for the protease subtilisin Carlsberg or its fragments with proteolytic activity or variants with proteolytic activity, including leader sequences thereof, is described. Also described are hybrid plasmids and microorganism strains which contain this structural gene, and a process for separating these microorganism strains from inactive strains. <IMAGE>
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3527913 | 1985-08-03 | ||
DE19853527913 DE3527913A1 (en) | 1985-08-03 | 1985-08-03 | ALKALINE PROTEASE, METHOD FOR PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS |
Publications (2)
Publication Number | Publication Date |
---|---|
AU6078986A true AU6078986A (en) | 1987-02-05 |
AU604640B2 AU604640B2 (en) | 1991-01-03 |
Family
ID=6277629
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU60789/86A Ceased AU604640B2 (en) | 1985-08-03 | 1986-08-01 | An alkaline protease, a process for the preparation of hybrid vectors and genetically transformed microorganisms |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0214435B1 (en) |
JP (1) | JPS6232888A (en) |
AT (1) | ATE78868T1 (en) |
AU (1) | AU604640B2 (en) |
DE (2) | DE3527913A1 (en) |
DK (1) | DK362686A (en) |
ZA (1) | ZA865787B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU604476B2 (en) * | 1986-01-15 | 1990-12-20 | Amgen, Inc. | Thermally stable and ph stable subtilisin analogs and method for production thereof |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK564086A (en) * | 1986-11-25 | 1988-06-17 | Novo Industri As | ENZYMATIC DETERGENT ADDITIVE |
AU620026B2 (en) * | 1987-02-27 | 1992-02-13 | Genencor International, Inc. | Molecular cloning and expression of genes encoding proteolytic enzymes |
JPH0787790B2 (en) * | 1987-03-04 | 1995-09-27 | 三菱瓦斯化学株式会社 | METHOD FOR PRODUCING NOVEL GENE DNA AND ALKALINE PROTEASE |
JPH0783716B2 (en) * | 1987-11-27 | 1995-09-13 | 昭和電工株式会社 | Gene system encoding alkaline protease |
DE3821491A1 (en) * | 1988-06-25 | 1989-12-28 | Henkel Kgaa | HYBRID PLASMIDE FOR THE PRODUCTION OF SUBTILISIN CARLSBERG IN BACILLUS |
DE3824827A1 (en) * | 1988-07-21 | 1990-02-01 | Henkel Kgaa | PLASMIDES FOR GENERATING ALPHA AMYLASE IN BACILLUS |
US5665587A (en) | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
US5658871A (en) * | 1989-07-07 | 1997-08-19 | Lever Brothers Company, Division Of Conopco, Inc. | Microbial lipase muteins and detergent compositions comprising same |
WO1991002803A1 (en) * | 1989-08-25 | 1991-03-07 | Henkel Research Corporation | A method of suppressing chromosomal gene expression |
DE3928046A1 (en) * | 1989-08-25 | 1991-02-28 | Henkel Kgaa | HYBRID PLASMIDE FOR THE PRODUCTION OF SUBTILISIN CARLSBERG IN BACILLUS |
EP0493398B1 (en) * | 1989-08-25 | 1999-12-08 | Henkel Research Corporation | Alkaline proteolytic enzyme and method of production |
US5482849A (en) * | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
US5275945A (en) * | 1991-10-08 | 1994-01-04 | Vista Chemical Company | Alkaline proteases stable in heavy-duty detergent liquids |
DE4231726A1 (en) * | 1992-09-23 | 1994-03-24 | Cognis Bio Umwelt | Mutated subtilisin-like serine proteases |
JP4044143B2 (en) | 1996-11-04 | 2008-02-06 | ノボザイムス アクティーゼルスカブ | Subtilase variants and compositions |
KR100591553B1 (en) | 1996-11-04 | 2006-06-19 | 노보자임스 에이/에스 | Subtilase variants and composition |
CN1148444C (en) | 1997-08-29 | 2004-05-05 | 诺沃奇梅兹有限公司 | Protease variants and compositions |
KR20010023468A (en) | 1997-08-29 | 2001-03-26 | 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 | Protease variants and compositions |
US6773907B2 (en) | 1997-11-21 | 2004-08-10 | Peter Kamp Hansen | Subtilase enzymes |
CA2310454C (en) | 1997-11-21 | 2012-01-24 | Novo Nordisk A/S | Protease variants and compositions |
EP1803817B1 (en) | 1998-12-18 | 2011-04-06 | Novozymes A/S | Subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid residue in an active site loop region |
DE60040282D1 (en) | 1999-05-20 | 2008-10-30 | Novozymes As | SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 132 AND 133 |
WO2000071690A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129 |
AU4393100A (en) | 1999-05-20 | 2000-12-12 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126 |
WO2000071687A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130 |
EP1183340B1 (en) | 1999-05-20 | 2008-09-03 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127 |
DE60040285D1 (en) | 1999-05-20 | 2008-10-30 | Novozymes As | SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN ITEM 127 AND 128 |
US6727085B2 (en) | 1999-12-15 | 2004-04-27 | Fanoe Tina Sejersgaard | Subtilase variants having an improved wash performance on egg stains |
US6777218B1 (en) | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
CN102766545B (en) | 2003-05-07 | 2014-08-06 | 诺维信公司 | Variant subtilisin enzymes (subtilases) |
CN102994486A (en) | 2003-10-23 | 2013-03-27 | 诺维信公司 | Protease with improved stability in detergents |
MX2008012999A (en) | 2006-04-14 | 2008-10-17 | Genencor Int | One-step treatment of textiles. |
JP2009534022A (en) | 2006-04-20 | 2009-09-24 | ノボザイムス アクティーゼルスカブ | A sabinase variant with improved detergency against egg stains |
JP5427776B2 (en) | 2007-04-30 | 2014-02-26 | ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン | Use of protein hydrolysates to stabilize metalloprotease detergent formulations |
SG148934A1 (en) | 2007-06-11 | 2009-01-29 | Novozymes As | A process for combined biopolishing and bleach clean-up |
EP2606146A1 (en) | 2010-08-19 | 2013-06-26 | Novozymes A/S | Induced sporulation screening method |
GB201212932D0 (en) * | 2012-07-20 | 2012-09-05 | Dupont Nutrition Biosci Aps | Method |
GB201212934D0 (en) * | 2012-07-20 | 2012-09-05 | Dupont Nutrition Biosci Aps | Method |
WO2020099490A1 (en) | 2018-11-14 | 2020-05-22 | Novozymes A/S | Oral care composition comprising enzymes |
CN116322625A (en) | 2020-08-24 | 2023-06-23 | 诺维信公司 | Oral care compositions comprising levanase |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3557002A (en) * | 1967-11-15 | 1971-01-19 | Procter & Gamble | Stabilized aqueous enzyme preparation |
GB1240058A (en) * | 1968-04-12 | 1971-07-21 | Procter & Gamble | Enzyme-containing detergent compositions |
CA1142105A (en) * | 1978-07-04 | 1983-03-01 | Peter Tang | Protease product of reduced allergenicity |
DE2925427C2 (en) * | 1979-06-23 | 1983-12-22 | Henkel KGaA, 4000 Düsseldorf | Process for the production of protease |
EP0108301B2 (en) * | 1982-11-01 | 1993-09-01 | SOLVAY ENZYMES, INC. (a Delaware corporation) | Method of heterologous cloning of gene in bacillus microorganism |
IE81141B1 (en) * | 1983-06-24 | 2000-04-05 | Genencor Int | Procaryotic carbonyl hydrolases |
JPS6030685A (en) * | 1983-07-29 | 1985-02-16 | Lion Corp | Recombinant dna, its preparation, bacillus subtilis containing the same, and preparation of protease using said bacillus subtilis |
JPS6112287A (en) * | 1984-06-26 | 1986-01-20 | Lion Corp | Recombinant dna, its preparation, bacterial strain containing same, preparation of exocytic secretion enzyme using same, and dna for promoting secretion of exocytic enzyme |
US4617265A (en) * | 1984-09-19 | 1986-10-14 | Board Of Regents, University Of Texas System | Colony blot assay for enterotoxigenic bacteria |
-
1985
- 1985-08-03 DE DE19853527913 patent/DE3527913A1/en not_active Withdrawn
-
1986
- 1986-07-26 AT AT86110342T patent/ATE78868T1/en not_active IP Right Cessation
- 1986-07-26 DE DE8686110342T patent/DE3686217D1/en not_active Revoked
- 1986-07-26 EP EP86110342A patent/EP0214435B1/en not_active Expired - Lifetime
- 1986-07-30 DK DK362686A patent/DK362686A/en not_active Application Discontinuation
- 1986-08-01 ZA ZA865787A patent/ZA865787B/en unknown
- 1986-08-01 AU AU60789/86A patent/AU604640B2/en not_active Ceased
- 1986-08-04 JP JP61184101A patent/JPS6232888A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU604476B2 (en) * | 1986-01-15 | 1990-12-20 | Amgen, Inc. | Thermally stable and ph stable subtilisin analogs and method for production thereof |
Also Published As
Publication number | Publication date |
---|---|
DK362686D0 (en) | 1986-07-30 |
EP0214435B1 (en) | 1992-07-29 |
EP0214435A3 (en) | 1990-01-17 |
ATE78868T1 (en) | 1992-08-15 |
AU604640B2 (en) | 1991-01-03 |
DE3686217D1 (en) | 1992-09-03 |
DE3527913A1 (en) | 1987-02-12 |
ZA865787B (en) | 1987-03-25 |
DK362686A (en) | 1987-02-04 |
EP0214435A2 (en) | 1987-03-18 |
JPS6232888A (en) | 1987-02-12 |
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