ZA202307267B - Use of resveratrol in cell electrotransfection and electrotransfection solution - Google Patents
Use of resveratrol in cell electrotransfection and electrotransfection solutionInfo
- Publication number
- ZA202307267B ZA202307267B ZA2023/07267A ZA202307267A ZA202307267B ZA 202307267 B ZA202307267 B ZA 202307267B ZA 2023/07267 A ZA2023/07267 A ZA 2023/07267A ZA 202307267 A ZA202307267 A ZA 202307267A ZA 202307267 B ZA202307267 B ZA 202307267B
- Authority
- ZA
- South Africa
- Prior art keywords
- electrotransfection
- resveratrol
- cell
- solution
- cells
- Prior art date
Links
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 title abstract 5
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 title abstract 5
- 229940016667 resveratrol Drugs 0.000 title abstract 5
- 235000021283 resveratrol Nutrition 0.000 title abstract 5
- 210000004027 cell Anatomy 0.000 abstract 4
- 241001465754 Metazoa Species 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The present invention belongs to the technical field of biology. Specifically disclosed is the use of resveratrol in cell electrotransfection, and further disclosed are an electrotransfection solution containing resveratrol and a method for cell electrotransfection. By means of the present invention, resveratrol is added to electrotransfected cells, and tests prove that the electrotransfection efficiency of fibroblasts can be improved, and the objective of improving the electrotransfection efficiency without influencing cell growth can be achieved. Resveratrol is naturally harmless, is safe to cells, and provides a safeguard for the use thereof in the birth of transgenic animals.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011568176.9A CN112725377B (en) | 2020-12-25 | 2020-12-25 | Application of resveratrol in electroporation transfected cells and electrotransfection liquid |
| PCT/CN2021/141333 WO2022135584A1 (en) | 2020-12-25 | 2021-12-24 | Use of resveratrol in cell electrotransfection and electrotransfection solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA202307267B true ZA202307267B (en) | 2024-02-28 |
Family
ID=75616623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA2023/07267A ZA202307267B (en) | 2020-12-25 | 2023-07-20 | Use of resveratrol in cell electrotransfection and electrotransfection solution |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN112725377B (en) |
| WO (1) | WO2022135584A1 (en) |
| ZA (1) | ZA202307267B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112725377B (en) * | 2020-12-25 | 2023-09-29 | 中国农业大学 | Application of resveratrol in electroporation transfected cells and electrotransfection liquid |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2192174B1 (en) * | 2008-11-21 | 2015-11-11 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Reprogramming cells toward a pluripotent state |
| GB201521101D0 (en) * | 2015-11-30 | 2016-01-13 | Koninklijke Nederlandse Akademie Van Wetenschappen | Transduction buffer |
| CN112725377B (en) * | 2020-12-25 | 2023-09-29 | 中国农业大学 | Application of resveratrol in electroporation transfected cells and electrotransfection liquid |
-
2020
- 2020-12-25 CN CN202011568176.9A patent/CN112725377B/en active Active
-
2021
- 2021-12-24 WO PCT/CN2021/141333 patent/WO2022135584A1/en active Application Filing
-
2023
- 2023-07-20 ZA ZA2023/07267A patent/ZA202307267B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022135584A1 (en) | 2022-06-30 |
| CN112725377B (en) | 2023-09-29 |
| CN112725377A (en) | 2021-04-30 |
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