WO2009120043A3 - Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells - Google Patents

Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells

Info

Publication number
WO2009120043A3
WO2009120043A3 PCT/KR2009/001581 KR2009001581W WO2009120043A3 WO 2009120043 A3 WO2009120043 A3 WO 2009120043A3 KR 2009001581 W KR2009001581 W KR 2009001581W WO 2009120043 A3 WO2009120043 A3 WO 2009120043A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
cd45
cells
mesenchymal stem
stem cells
cell culture
Prior art date
Application number
PCT/KR2009/001581
Other languages
French (fr)
Korean (ko)
Other versions
WO2009120043A2 (en )
Inventor
손영숙
홍현숙
안우성
Original Assignee
경희대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0663Bone marrow mesenchymal stem cells (BM-MSC)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0665Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/11Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1157Monocytes, macrophages

Abstract

The present invention relates to a use for CD45+ cells or a CD45+ cell culture fluid for promoting the growth of mesenchymal stem cells; a composition for promoting the growth of mesenchymal stem cells comprising CD45+ cells or a CD45+ cell culture fluid; and a culturing method for promoting the growth of mesenchymal stem cells by adding CD45+ cells or a CD45+ cell culture fluid to mesenchymal stem cells and allowing culturing to proceed. If culturing is carried out by adding CD45+ cells or a CD45+ cell culture fluid to mesenchymal stem cells, then the colony-forming ability of the mesenchymal stem cells is increased by at least three times as compared with culturing of mesenchymal stem cells alone. Moreover, CD29+ cells which have been cultured with treatment by CD45+ cells or a CD45+ cell culture fluid maintain, unchanged, the morphological characteristics of mesenchymal stem cells, cell markers, and inherent characteristics such as pluripotency. Thus CD45+ cells or a CD45+ cell culture fluid can be used to promote the growth of mesenchymal stem cells, and a culturing method in which CD45+ cells or a CD45+ cell culture fluid is added to mesenchymal stem cells and culturing is allowed to proceed can be used to grow mesenchymal stem cells in sufficient number to allow them to be used in cell therapy.
PCT/KR2009/001581 2008-03-28 2009-03-27 Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells WO2009120043A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR10-2008-0029244 2008-03-28
KR20080029244 2008-03-28

Publications (2)

Publication Number Publication Date
WO2009120043A2 true WO2009120043A2 (en) 2009-10-01
WO2009120043A3 true true WO2009120043A3 (en) 2009-12-30

Family

ID=41114475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001581 WO2009120043A3 (en) 2008-03-28 2009-03-27 Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells

Country Status (2)

Country Link
KR (1) KR101077042B1 (en)
WO (1) WO2009120043A3 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101373100B1 (en) * 2013-05-03 2014-03-13 재단법인 통합의료진흥원 Compostions for promoting proliferation of bone marrow stem cell comprising angelica extract

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005113749A2 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Stem cell populations and methods of use
KR20060016133A (en) * 2006-02-02 2006-02-21 김병수 The development of human placenta stromal cells to promote differentiation of human embryonic stem cells and tembryoid bodieso to hemopoietic stem cells and the culture technique for differentiation of human embryonic stem cells and embryoid bodies to hemopoietic stem cells
KR20060058476A (en) * 2004-11-25 2006-05-30 고려대학교 산학협력단 Method for promoting the differentiation of embryonic bodies to hemopoietic stem cells using human bone marrow stromal cells
KR20070001171A (en) * 2004-02-06 2007-01-03 테라다임, 인코포레이티드 Compositions and methods relating to culturing neural stem cells with bone marrow stromal cells
WO2007027156A1 (en) * 2005-09-02 2007-03-08 Agency For Science, Technology And Research Method of deriving mesenchymal stem cells
US20070092967A1 (en) * 2003-11-11 2007-04-26 Hoon Han Method of isolating and culturing mesenchymal stem cell derived from umbilical cord blood

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070092967A1 (en) * 2003-11-11 2007-04-26 Hoon Han Method of isolating and culturing mesenchymal stem cell derived from umbilical cord blood
KR20070001171A (en) * 2004-02-06 2007-01-03 테라다임, 인코포레이티드 Compositions and methods relating to culturing neural stem cells with bone marrow stromal cells
US20050265980A1 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Cell culture environments for the serum-free expansion of mesenchymal stem cells
WO2005113749A2 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Stem cell populations and methods of use
KR20060058476A (en) * 2004-11-25 2006-05-30 고려대학교 산학협력단 Method for promoting the differentiation of embryonic bodies to hemopoietic stem cells using human bone marrow stromal cells
WO2007027156A1 (en) * 2005-09-02 2007-03-08 Agency For Science, Technology And Research Method of deriving mesenchymal stem cells
KR20060016133A (en) * 2006-02-02 2006-02-21 김병수 The development of human placenta stromal cells to promote differentiation of human embryonic stem cells and tembryoid bodieso to hemopoietic stem cells and the culture technique for differentiation of human embryonic stem cells and embryoid bodies to hemopoietic stem cells

Also Published As

Publication number Publication date Type
KR101077042B1 (en) 2011-10-26 grant
WO2009120043A2 (en) 2009-10-01 application
KR20090103828A (en) 2009-10-01 application

Similar Documents

Publication Publication Date Title
Ruibal et al. Phylogeny of rock-inhabiting fungi related to Dothideomycetes
De-Bashan et al. Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense
Dang et al. Microbial surface colonization and biofilm development in marine environments
Sakai et al. Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage ‘Rice Cluster I’, and proposal of the new archaeal order Methanocellales ord. nov.
Antal et al. Production of H2 by sulphur‐deprived cells of the unicellular cyanobacteria Gloeocapsa alpicola and Synechocystis sp. PCC 6803 during dark incubation with methane or at various extracellular pH
Blair et al. Light and growth medium effect on Chlorella vulgaris biomass production
Cho et al. Enhancing microalgal biomass productivity by engineering a microalgal–bacterial community
Tate et al. The effects of plant growth substances and mixed cultures on growth and metabolite production of green algae Chlorella sp.: a review
Qi et al. Study of the siderophore‐producing Trichoderma asperellum Q1 on cucumber growth promotion under salt stress
Dissanayaka et al. Characterization of dental pulp stem cells isolated from canine premolars
Zhu Microalgal culture strategies for biofuel production: a review
Tang et al. Effect of dilution rate on the microbial structure of a mesophilic butyrate-degrading methanogenic community during continuous cultivation
Dhami et al. Synergistic role of bacterial urease and carbonic anhydrase in carbonate mineralization
Ebrahimian et al. Lipid production in mixotrophic cultivation of Chlorella vulgaris in a mixture of primary and secondary municipal wastewater
Miura et al. Evaluation of marine sediments as microbial sources for methane production from brown algae under high salinity
Ziervogel et al. Changes in the spectrum and rates of extracellular enzyme activities in seawater following aggregate formation
Tsuji et al. An application of wastewater treatment in a cold environment and stable lipase production of Antarctic basidiomycetous yeast Mrakia blollopis
Correia et al. Combining hypoxia and bioreactor hydrodynamics boosts induced pluripotent stem cell differentiation towards cardiomyocytes
Tan et al. Immunomodulative effects of mesenchymal stem cells derived from human embryonic stem cells in vivo and in vitro
Karlsson et al. Archaeal abundance in relation to root and fungal exudation rates
Parshina et al. Methanospirillum stamsii sp. nov., a psychrotolerant, hydrogenotrophic, methanogenic archaeon isolated from an anaerobic expanded granular sludge bed bioreactor operated at low temperature
Guo et al. Microalgae cultivation using an aquaculture wastewater as growth medium for biomass and biofuel production
Santos et al. Microalgal symbiosis in biotechnology
WO2008118820A3 (en) Somatic cell reprogramming
Sato et al. Seasonal variation of biomass and oil production of the oleaginous diatom Fistulifera sp. in outdoor vertical bubble column and raceway-type bioreactors

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09723740

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase in:

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09723740

Country of ref document: EP

Kind code of ref document: A2