WO2009120043A3 - Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells - Google Patents

Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells

Info

Publication number
WO2009120043A3
WO2009120043A3 PCT/KR2009/001581 KR2009001581W WO2009120043A3 WO 2009120043 A3 WO2009120043 A3 WO 2009120043A3 KR 2009001581 W KR2009001581 W KR 2009001581W WO 2009120043 A3 WO2009120043 A3 WO 2009120043A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
cells
mesenchymal
stem
cell
culture
Prior art date
Application number
PCT/KR2009/001581
Other languages
French (fr)
Korean (ko)
Other versions
WO2009120043A2 (en )
Inventor
손영숙
홍현숙
안우성
Original Assignee
경희대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0663Bone marrow mesenchymal stem cells (BM-MSC)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0665Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/11Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1157Monocytes, macrophages

Abstract

The present invention relates to a use for CD45+ cells or a CD45+ cell culture fluid for promoting the growth of mesenchymal stem cells; a composition for promoting the growth of mesenchymal stem cells comprising CD45+ cells or a CD45+ cell culture fluid; and a culturing method for promoting the growth of mesenchymal stem cells by adding CD45+ cells or a CD45+ cell culture fluid to mesenchymal stem cells and allowing culturing to proceed. If culturing is carried out by adding CD45+ cells or a CD45+ cell culture fluid to mesenchymal stem cells, then the colony-forming ability of the mesenchymal stem cells is increased by at least three times as compared with culturing of mesenchymal stem cells alone. Moreover, CD29+ cells which have been cultured with treatment by CD45+ cells or a CD45+ cell culture fluid maintain, unchanged, the morphological characteristics of mesenchymal stem cells, cell markers, and inherent characteristics such as pluripotency. Thus CD45+ cells or a CD45+ cell culture fluid can be used to promote the growth of mesenchymal stem cells, and a culturing method in which CD45+ cells or a CD45+ cell culture fluid is added to mesenchymal stem cells and culturing is allowed to proceed can be used to grow mesenchymal stem cells in sufficient number to allow them to be used in cell therapy.
PCT/KR2009/001581 2008-03-28 2009-03-27 Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells WO2009120043A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR10-2008-0029244 2008-03-28
KR20080029244 2008-03-28

Publications (2)

Publication Number Publication Date
WO2009120043A2 true WO2009120043A2 (en) 2009-10-01
WO2009120043A3 true true WO2009120043A3 (en) 2009-12-30

Family

ID=41114475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001581 WO2009120043A3 (en) 2008-03-28 2009-03-27 Use for cd45+ cells or a cd45+ cell culture fluid for promoting the growth of mesenchymal stem cells

Country Status (2)

Country Link
KR (1) KR101077042B1 (en)
WO (1) WO2009120043A3 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101373100B1 (en) * 2013-05-03 2014-03-13 재단법인 통합의료진흥원 Compostions for promoting proliferation of bone marrow stem cell comprising angelica extract

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005113749A2 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Stem cell populations and methods of use
KR20060016133A (en) * 2006-02-02 2006-02-21 김병수 The development of human placenta stromal cells to promote differentiation of human embryonic stem cells and tembryoid bodieso to hemopoietic stem cells and the culture technique for differentiation of human embryonic stem cells and embryoid bodies to hemopoietic stem cells
KR20060058476A (en) * 2004-11-25 2006-05-30 고려대학교 산학협력단 Method for promoting the differentiation of embryonic bodies to hemopoietic stem cells using human bone marrow stromal cells
KR20070001171A (en) * 2004-02-06 2007-01-03 테라다임, 인코포레이티드 Compositions and methods relating to culturing neural stem cells with bone marrow stromal cells
WO2007027156A1 (en) * 2005-09-02 2007-03-08 Agency For Science, Technology And Research Method of deriving mesenchymal stem cells
US20070092967A1 (en) * 2003-11-11 2007-04-26 Hoon Han Method of isolating and culturing mesenchymal stem cell derived from umbilical cord blood

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070092967A1 (en) * 2003-11-11 2007-04-26 Hoon Han Method of isolating and culturing mesenchymal stem cell derived from umbilical cord blood
KR20070001171A (en) * 2004-02-06 2007-01-03 테라다임, 인코포레이티드 Compositions and methods relating to culturing neural stem cells with bone marrow stromal cells
US20050265980A1 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Cell culture environments for the serum-free expansion of mesenchymal stem cells
WO2005113749A2 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Stem cell populations and methods of use
KR20060058476A (en) * 2004-11-25 2006-05-30 고려대학교 산학협력단 Method for promoting the differentiation of embryonic bodies to hemopoietic stem cells using human bone marrow stromal cells
WO2007027156A1 (en) * 2005-09-02 2007-03-08 Agency For Science, Technology And Research Method of deriving mesenchymal stem cells
KR20060016133A (en) * 2006-02-02 2006-02-21 김병수 The development of human placenta stromal cells to promote differentiation of human embryonic stem cells and tembryoid bodieso to hemopoietic stem cells and the culture technique for differentiation of human embryonic stem cells and embryoid bodies to hemopoietic stem cells

Also Published As

Publication number Publication date Type
KR101077042B1 (en) 2011-10-26 grant
WO2009120043A2 (en) 2009-10-01 application
KR20090103828A (en) 2009-10-01 application

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