ZA202207465B - Three-dimensional dna nanotweezer for simultaneously detecting ochratoxin a and zearalenone and preparation method and use thereof - Google Patents

Three-dimensional dna nanotweezer for simultaneously detecting ochratoxin a and zearalenone and preparation method and use thereof

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Publication number
ZA202207465B
ZA202207465B ZA2022/07465A ZA202207465A ZA202207465B ZA 202207465 B ZA202207465 B ZA 202207465B ZA 2022/07465 A ZA2022/07465 A ZA 2022/07465A ZA 202207465 A ZA202207465 A ZA 202207465A ZA 202207465 B ZA202207465 B ZA 202207465B
Authority
ZA
South Africa
Prior art keywords
nanotweezer
zearalenone
preparation
simultaneously detecting
dimensional dna
Prior art date
Application number
ZA2022/07465A
Inventor
Zhixian Gao
Shuyue Ren
Ruipeng Chen
Huanying Zhou
Yuan Peng
Jialei Bai
Shuang Li
Dianpeng Han
Kang Qin
Yu Wang
Sen Li
Xuexia Jia
Original Assignee
Inst Of Environmental Medicine And Occupational Medicine Academy Of Military Medicine Academy Of Mil
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Of Environmental Medicine And Occupational Medicine Academy Of Military Medicine Academy Of Mil filed Critical Inst Of Environmental Medicine And Occupational Medicine Academy Of Military Medicine Academy Of Mil
Publication of ZA202207465B publication Critical patent/ZA202207465B/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA2022/07465A 2022-05-09 2022-07-06 Three-dimensional dna nanotweezer for simultaneously detecting ochratoxin a and zearalenone and preparation method and use thereof ZA202207465B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210497473.1A CN114875034A (en) 2022-05-09 2022-05-09 Three-dimensional DNA nano-tweezers for simultaneously detecting ochratoxin A and zearalenone as well as preparation method and application of three-dimensional DNA nano-tweezers

Publications (1)

Publication Number Publication Date
ZA202207465B true ZA202207465B (en) 2022-07-27

Family

ID=82558320

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2022/07465A ZA202207465B (en) 2022-05-09 2022-07-06 Three-dimensional dna nanotweezer for simultaneously detecting ochratoxin a and zearalenone and preparation method and use thereof

Country Status (2)

Country Link
CN (1) CN114875034A (en)
ZA (1) ZA202207465B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116466090B (en) * 2023-03-27 2024-05-31 江南大学 Double-function DNA tweezers nano biosensor and preparation method and application thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103013998B (en) * 2012-11-22 2014-05-28 江南大学 Oligonucleotides aptamer special for distinguishing zearalenone
CN108219783A (en) * 2017-12-27 2018-06-29 温州大学 Fluorescent silver nanocluster, preparation method and its application in Mycotoxin identification of nucleic acid stability
CN110115992B (en) * 2019-06-06 2021-06-01 福州大学 Aptamer functionalized polymer column for specific recognition of mycotoxin and preparation method thereof
CN110261362B (en) * 2019-08-06 2020-08-04 青岛农业大学 Biosensor for simultaneously detecting zearalenone and ochratoxin A as well as preparation method and detection method thereof
CN110484605B (en) * 2019-09-23 2020-07-28 中国人民解放军陆军军医大学第一附属医院 Gene for in-situ detection of microRNA-34 by living cells and preparation method and application thereof
CN113238036B (en) * 2021-02-01 2022-03-11 温州医科大学 Ochratoxin A double-color fluorescence detection method based on hybridization chain reaction and DNA tweezers
CN113552188B (en) * 2021-07-23 2022-06-10 济南大学 Electrochemical biosensor for detecting ochratoxin A based on DNA tetrahedron
CN113804898B (en) * 2021-09-18 2023-07-07 军事科学院军事医学研究院环境医学与作业医学研究所 Fluorescence sensing method and kit for simultaneously detecting cortisol, serum testosterone and creatine kinase isoenzyme
CN114354909A (en) * 2021-12-08 2022-04-15 军事科学院军事医学研究院环境医学与作业医学研究所 DNA origami-aptamer nano-array sensor and preparation method and application thereof

Also Published As

Publication number Publication date
CN114875034A (en) 2022-08-09

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