ZA200700602B - Method and compositions useful for preventing and/or treating sensitive and/or dry skin - Google Patents

Description

Method and compositions useful for preventing and/or treating sensitive and/or dry skin

The present invention relates to a method of cosmetic treatment and to a composition, in particular a cosmetic and/or dermatological composition, intended more particularly for preventing and/or treating skin described as sensitive and/or dry skin.

In general, sensitive skin is defined by, a specific reactivity of the skin. However, as opposed to skin described as allergic, this reactivity is nébt the result of an immunological process, i.e. it does not occur only in skin that is already sensitized, in response to the presence of an allergen. Its mechanism is said to be aspecific.

This skin reactivity generally results in the manifestation of signs of discomfort in response to the subject coming into contact with a triggering element that may have various origins. It may involve the application of a cosmetic product at the surface of the sensitive skin, food intake, or exposure to abrupt temperature variations, to atmospheric pollution and/or to ultraviolet or infrared rays. Associated factors such as age and the type of skin also exist. Thus, sensitive skin is more common in the case of dry or oily skin than in the case of normal skin.

The appearance of these signs of discomfort, which appear within minutes after the individual has come into contact with the triggering element, is one of the essential characteristics of sensitive skin. These signs are essentially dysaesthetic sensations. The term “dysaesthetic sensations” is intended to mean more or less painful sensations experienced in a region of skin, such as stinging, tingling, itching or pruritus, burning, heating, discomfort, tautness, etc. These subjective signs most commonly exist in the absence of visible chemical signs such as redness and desquamations. At this time, it is known that these skin irritation and intolerance reactions are in particular associated with a release of neuropeptides by the nerve endings in the epidermis and the dermis.

In document EP 737 471, it has been shown that the incorporation of certain alkaline earth metal salts into a cosmetic and/or dermatological composition makes it possible to effectively oppose the release of these neuropeptides and to advantageously obtain a preventive and/or curative effect for sensitive skin. Document EP 806 933 illustrates more particularly the effectiveness of strontium salts for treating irritable skin.

However, there is, to date, still no completely satisfactory solution available for preventing and/or treating this type of skin described as sensitive, and this problem is more particularly exacerbated when this sensitive skin is associated with dry skin. Dry skin essentially manifests itself through a sensation of tautness and/or of tension and it is often associated with a decrease in the level of moisturization of the skin and an impairment of the barrier function, measured through an imperceptible loss of water.

Document WO 02/28402, for its part, describes the fact that probiotic microorganisms can also have a beneficial effect in the regulation of skin hypersensitivity reactions such as inflammatory and allergic reactions that are the result of an immunological process as opposed to the reactivity of sensitive skin. Also reported, in “Probiotics in the management of atopic eczema, Clinical and Experimental

Allergy 20007, Volume 30, pages 1604-1610, is a study concerning the effect of probiotics on immune mechanisms in infants, for instance atopic dermatitis.

As regards document EP 110 550, it proposes using bacterial agents, in particular of the probiotic type, for stabilizing -

and/or regulating the skin ecoflora. These compounds are described therein as being effective for preventing the adhesion of pathogenic flora to the skin.

Unexpectedly, the inventors have noted that these microorganisms, in particular probiotic microorganisms, can prove to be effective, in particular in adults, for treating sensitive skin in particular associated with dry skin, with the proviso that they are combined with an effective amount of at least one divalent inorganic cation.

This effectiveness is all the more unexpected since these two types of active agents were, to date, presumed to act via two completely different methods of action that generally involved a method of administration specific to each of them, i.e. mainly oral for the microorganisms, in particular probiotic microorganisms, and mainly topical for the divalent inorganic cations, and in particular for alkaline earth metal salts. What is more, there was nothing to predict their effectiveness with respect to sensitive skin associated with dry skin.

Consequently, according to a first of its aspects, the present invention relates to a method of cosmetic treatment for preventing and/or treating sensitive and/or dry skin, comprising the oral administration of at least an effective amount of at least one microorganism, in particular probiotic microorganism, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one divalent inorganic cation.

Tt also relates to a method comprising the oral or topical administration of at least an effective amount of at least one microorganism, in particular probiotic microorganism, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one divalent inorganic cation in the form of a salt other than a sulphate.

According to another of its aspects, the present invention also relates to the use of an effective amount of at least one microorganism, in particular probiotic microorganism, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one divalent inorganic cation, for preparing a composition intended for oral administration and useful for preventing and/or treating sensitive and/or dry skin.

According to a first variant, this combination is formulated in the form of a food supplement, or even a foodstuff.

According to a second variant, it is in the form of a cosmetic and/or dermatological composition according to the invention.

The present invention is also directed towards the use, as an agent intended to prevent and/or treat sensitive and/or dry skin, of at least an effective amount of at least one microorganism, in particular probiotic microorganism, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one divalent inorganic cation in the form of a salt other than a sulphate.

According to another of its aspects, the present invention relates to a cosmetic and/or dermatological composition, that is in particular useful for preventing and/or treating sensitive and/or dry skin, comprising, in a physiologically acceptable carrier, at least an effective amount of at least one microorganism, in particular probiotic microorganism, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least two alkaline earth metals in the form of anhydrous or hydrated organic salts, or of chelated complexes.

According to another of its aspects, the present invention

§ relates to a cosmetic and/or dermatological composition, that is in particular useful for preventing and/or treating sensitive and/or dry skin, comprising, in a physiologically acceptable carrier, at least an effective amount of at least one microorganism, in particular probiotic microorganism, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one alkaline earth metal in the form of a salt chosen from bicarbonates, glycerophosphates, nitrates, acetates, hydroxides, o-hydroxy acid salts, such as citrates, tartrates, lactates and malates, or salts of fruit acids, amino acid salts, such as aspartates, arginates and fumarates, or fatty acid salts such as palmitates, oleates, caseinates and behenates.

Sensitive and/or dry skin

As specified above, sensitive skin is different from allergic skin. Its reactivity is not the result of an immunological process and generally only results in dysaesthetic sensations.

For obvious reasons, the lack of visible signs makes it difficult to diagnose sensitive skin. Most commonly, this diagnosis is based on the results of questioning the patient.

This symptomology also has the advantage of making it possible to differentiate between sensitive skin possibly associated with dry skin, and contact irritation or allergy for which, on the other hand, visible inflammatory signs exist.

Consequently, the development of “sensitive skin” products has required the provision of tools for evaluating the sensory reaction of the skin. The first tools were, right from their conception, inspired by the essential characteristic of sensitive skin, i.e. the presence of signs of discomfort induced by a topical application. Thus, the lactic acid “stinging test” was the first test proposed. It is carried out by recording the stinging sensations reported by a volunteer after application of a 10% lactic acid solution to the alar processes of the nose. The individuals who report moderate or strong stinging sensations are called “stingers” and are considered to have sensitive skin. Because of this skin sensitivity to topical product application, these individuals are then selected for testing “sensitive skin” products. More recently, in order to specifically activate the peripheral nerve endings involved in the discomfort and called nociceptors, recently identified as being involved in sensitive skin, new tests have been proposed that as a matter of fact use other discomfort inducers such as capsaicin.

This second type of test, described in application

EP 1 374 913, also constitutes another particularly useful tool for diagnosing sensitive skin.

For the purpose of the present invention, the term “sensitive skin” covers irritable skin and intolerant skin.

Intolerant skin is skin that reacts to various factors, such as the application of cosmetic or dermatological products or of soap, . through sensations of heating, of tautness, of tingling and/or of redness. In general, these signs are associated with erythema and with hyperseborrhoeic or acneic skin, or even skin exhibiting rosacea, with or without sores.

Irritable skin is skin which reacts through pruritus, i.e. through itching or prickling, to various factors such as the environment, emotions, foods, the wind, rubbing, shaving, hard water with a high calcium concentration, temperature variations or wool.

In general, these two types of skin may be associated with dryness of the skin with or without sores or with skin that exhibits erythema.

As specified above, dryness of the skin is often associated with a decrease in the level of moisturization of the skin, evaluated by corneometry, and with an impairment of the

S barrier function, measured through the imperceptible loss of water.

Dry skin essentially manifests itself through a sensation of tautness and/or tension. Said skin is also rough to the touch and appears to be covered with scales. When the skin is slightly dry, these scales are abundant but not very visible to the naked eye. When this condition worsens, there are increasingly fewer of these scales but they are increasingly visible to the naked eye.

The cause of this dryness of the skin may be of the constitutional or acquired type.

In the case of acquired dry skin, the involvement of outside parameters such as exposure to chemical agents, ‘to difficult climatic conditions or to sunlight or alternatively certain therapeutic treatments (retinoids, for example) is determined. Under these outside influences, the skin can then become momentarily and locally dry. This can involve any type of normal and even oily skin.

In the case of constitutional dry skin, two categories can be distinguished: pathological skin and non-pathological skin.

Pathological constitutional dry skin is essentially represented by atopic dermatitis and ichthyoses. It is virtually independent of the outside conditions.

Atopic dermatitis is described as being associated with a deficiency in metabolism of the lipids of the stratum corneum, and in particular of the ceramides. This pathology presents itself in the form of more or less chronic xerosis concerning a large extent of the body, associated with inflammatory and pruriginous exacerbations in plaques.

Ichthyoses are pathologies characterized by a genetic deficiency that affects the keratinization process at various stages. It manifests itself through considerable desquamation in plaques.

Non-pathological constitutional dry skin is dry skin for which the severity can depend on the outside factors already mentioned. Senile skin (characterized by a general decrease in metabolism in the skin with age), fragile skin (very sensitive to outside factors and often accompanied by erythema and rosacea) and common xerosis (of probable genetic origin and manifesting itself predominantly on the face, the limbs and the back of the hands) enter into this skin category.

The compositions and method according to the invention are thus found to be most particularly effective for preventing and/or treating sensitive and/or dry skin, and more particularly skin referred to as reactive, irritable and/or intolerant, acquired dry skin and/or constitutional dry skin.

Microorganisms and in particular probiotic microorganisms

The microorganisms that are suitable for the invention are microorganisms that can be administered without risks to animals or humans.

In particular, in the present invention, at least one “probiotic-type” microorganism is used.

For the purpose of the present invention, the term “probiotic microorganism” is intended to mean a living microorganism that, when it is consumed in an appropriate amount, has a positive effect on the health of its host “joint FAO/WHO

Expert Consultation on Evaluation of Health and Nutritional

Properties of Probiotic in Food Including Powder Milk with

Live Lactic Acid Bacteria, 6 October 2001”, and which can in particular improve the intestinal microbial balance.

For the purpose of the invention, the term “metabolite” denotes any substance that is derived from the metabolism of the microorganisms considered according to the invention and that is also effective for treating sensitive skin.

For the purpose of the invention, the term “fraction” denotes more particularly a fragment of said microorganism that is effective for treating dry skin by analogy with said whole microorganism.

The microorganisms that are suitable for the invention can be chosen in particular from ascomycetes such as Saccharomyces,

Yarrowia, Kluyveromyces, Torulaspora, Schizosaccharomyces pombe, Debaromyces, Candida, Pichia, Aspergillus and

Penicillium, bacteria of the genus Bifidobacterium,

Bacteroides, Fusobacterium, Melissococcus, Propionibacterium,

Enterococcus, Lactococcus, Staphylococcus, Peptostrepococcus,

Bacillus, Pediococcus, Micrococcus, Leuconostoc, Weissella,

Aerococcus, Oenococcus and Lactobacillus and mixtures thereof.

As ascomycetes that are most particularly suitable for the present invention, mention may in particular be made of

Yarrowia lipolitica and Kluyveromyces lactis, along with - 30 Saccharomyces cereviseae, Torulaspora, Schizosaccharomyces pombe, Candida and Pichia.

As regard probiotic microorganisms, the following genus of bacteria and of straim are generally used: - The lactic bacteries like: . Lactobacillus species: acidophilus (LCl, NCFB 1748) ;

amylovorus, casei (Shirota), rhamnosus (strain GG), brevis, crispatus, delbrueckii (subsp bulgaricus, lactis)), fermentum, helveticus, gallinarum, gasseri, johnsonii, paracasei, plantarum, reuteri, rhamnosus, salivarius . Cocci: Enterococcus (faecalis, faecium), Lactococcus lactis {subspp lactis or cremoris), Leuconstoc mesenteroides subsp dextranicum, Pediococcus acidilactici, Sporolactobacillus inulinus, Streptococcus salvarius subsp. Thermophilus. . - The Bifidobacterium species like Bifidobacterium adolescentis, animalis, bifidum, breve, lactis, longum, infantis. - The strain Saccharomyces cerevisiae, and ~ The Dbacteries like Bacillus (cereus var toyo or subtilis), Bacillus coagulans, B licheniformis,

Escherichia coli strain nissle, Propionibacterium freudenreichii.

Specific examples of probiotic microorganisms are

Bifidobacterium bifidum, Bifidobacterium infantis,

Bifidobacterium longum, Lactobacillus acidophilus,

Lactobacillus alimentarius, Lactobacillus casei subsp. Casei,

Lactobacillus casei Shirota, Lactobacillus paracasei,

Lactobacillus curvatus, Lactobacillus delbruckii subsp.

Lactis, Lactobacillus gasseri, Lactobacillus johnsonii,

Lactobacillus reuteri, Lactobacillus rhamnosus (Lactobacillus

GG), Lactobacillus sake, Lactococcus lactis, Streptococcus thermophilus, Staphylococcus carnosus and Staphylococcus xylosus, and mixtures thereof.

The microorganisms may be formulated in powdered form, i.e. in a dry form, or in the form of suspensions or solutions.

If necessary, these microorganisms may be formulated within the compositions according to the invention in an encapsulated form so as to significantly improve their survival time. In such a case, the presence of a capsule can in particular delay or prevent degradation of the microorganism in the gastrointestinal tract.

More particularly, the microorganisms are probiotic microorganisms derived from the group of lactic acid bacteria, such as in particular Lactobacillus and/or

Bifidobacterium. By way of illustration of these lactic acid bacteria, mention may more particularly be made of

Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus casei or

Bifidobacterium bifidum, Bifidobacterium breve,

Bifidobacterium longum, Bifidobacterium animalis,

Bifidobacterium lactis, Bifidobacterium infantis,

Bifidobacterium adolescentis or Bifidobacterium pseudocatenulatum, and mixtures thereof.

The most particularly suitable species are Lactobacillus johnsonii, Lactobacillus paracasei, Bifidobacterium adolescentis, Bifidobacterium longum, and Bifidobacterium lactis NCC 2818 [also entitled (Bb 12) (ATCC27536) ] respectively deposited according to the Treaty of Budapest with the Pasteur Institute (28 rue du Docteur Roux, F-75024

Paris cedex 15) on 30/06/92, 12/01/99, 15/04/99, 15/04/99 and 07/06/2005 under the following designations: CNCM I-1225,

CNCM I-2116, CNCM I-2168, and CNCM I-2170 and CNCM I-3446, and the genus Bifidobacterium longum (BB536) . The

Bifidobacterium lactis (CNCM I-3446) strain can be obtained from Hansen (Chr. Bansen A/S, 10-12 Boege Alle, P.O. Box 407,

DK-2970 Hoersholm, Denmark).

According to a particular embodiment of the invention, the composition comprises at least two microorganisms, in particular probiotic microorganisms, and/or metabolites and/or fractions, that are different. These microorganisms can differ in terms of their nature, for example bacterium and fungus, or else in terms of family, their genus or their species, or only in terms of their strain.

The composition according to the invention may thus comprise at least one microorganism chosen from those mentioned above and a second microorganism that may or may not also be chosen from these microorganisms.

According to a variant of the invention, the composition contains at least one Lactobacillus sp microorganism and at least one Bifidobacterium sp microorganism, in particular in equivalent amounts, and more particularly in a proportion of 101° cfu respectively.

The microorganisms and/or their fractions and/or metabolites can be formulated in an appropriate carrier in an amount of at least 10° cfu/g, in particular at doses ranging from 10° to 10% cfu/g, and more particularly from 107 to 10%? cfu/g of carrier.

In general, the compositions according to the invention, and in particular those intended to be administered orally, can comprise, for the live microorganisms, from 10° to 10'° cfu/g, in particular from 10° to 10*® cfu/g, and more particularly from 107 to 10? cfu/g of microorganisms per gram of carrier, or at equivalent calculated doses for inactive or dead microorganisms or for microorganism fractions or for produced metabolites. The compositions for topical application according to the invention generally comprise from 10° to 10'2 cfu/g, in particular form 10° to 10° cfu/g, and more particularly from 107 to 10° cfu/g of microorganisms, in particular probiotic microorganisms.

When the composition comprises metabolites, the metabolite contents in the compositions correspond substantially to the contents that can be produced by 10° to 10% cfu, in particular 10° to 10% cfu, and more particularly 107 to 10} cfu of live microorganisms per gram of carrier.

In the particular case of the compositions that have to be administered orally, the concentration of microorganism(s), in particular probiotic microorganism(s), can be adjusted so as to correspond to doses (expressed as microorganism equivalent) ranging from 5 X 10° to 10% c¢fu/d, and in particular from 10% to 10%Y%cfu/d.

The microorganism(s) can be included in the composition according to the invention in a live, semi-active or inactivated, or dead form.

It (they) may also be included in the form of fractions of cell components or in the form of metabolites. The microorganism(s), metabolite(s) or fraction(s) can also be introduced in the form of a lyophilized powder or of a culture supernatant and/or, where appropriate, in a concentrated form.

In the particular case of the topical compositions, it may be advantageous to use these microorganisms in inactivated, or even dead, form.

Divalent inorganic cation

According to the invention, one or more divalent inorganic cation (s) may be used.

In particular, in the present invention, at least two, or even three, different divalent inorganic cations are used.

In the context of the present invention, the divalent inorganic cations can be used in various forms. The divalent inorganic cation can thus be in the form of an anhydrous or hydrated inorganic or organic salt, or of a chelated complex.

The salts may, for example, be carbonates, bicarbonates,

sulphates, glycerophosphates, chlorides, nitrates, acetates, hydroxides, oxides, o-hydroxy acid salts (citrates, tartrates, lactates, malates) or salts of fruit acids, or else amino acid salts (aspartate, arginate, fumarate) or fatty acid salts (palmitate, oleate, caseinate, behenate).

According to a particular embodiment, the divalent inorganic cation is chosen from manganese, copper and/or zinc.

According to another particular embodiment, the divalent inorganic cation is an alkaline earth metal. As alkaline earth metals that can be used in the invention, mention may be made of barium, calcium, magnesium, strontium and/or beryllium.

Advantageously, the divalent inorganic cation, and in particular alkaline earth metal, is used, in the present invention, in the form of a salt. In particular, the salt may be chosen from calcium nitrate, strontium nitrate, magnesium gluconate, calcium lactate, strontium gluconate, magnesium lactate, calcium chloride, strontium chloride, magnesium chloride, calcium carbonate, strontium sulphate, magnesium sulphate, calcium glycerophosphate, calcium citrate, magnesium citrate, strontium acetate and magnesium acetate, and mixtures thereof.

According to a particularly advantageous embodiment, at least one divalent inorganic cation chosen from strontium citrate, chloride, gluconate, sulphate, lactate and/or acetate, calcium citrate, chloride, gluconate, sulphonate, lactate and/or acetate and/or magnesium citrate, chloride, gluconate, sulphate, lactate and/or acetate salts, and mixtures thereof, is used.

According to one embodiment, the methods used and the compositions according to the invention use at least two divalent inorganic cations, and in particular two alkaline earth metals, that are in particular different, in the form of organic salts.

The divalent inorganic cation can also be used in the form of a chelated complex, in particular chelated with crystalline or ionized proteins.

The divalent inorganic cation may also be in a specific form that is stored by a microorganism, for example of the yeast type, like selenium.

Thus, the cations can be introduced as they are into the compositions according to the invention, or by means of a compound or mixture of compound(s) known to contain a high concentration of at least one of these cations. For example, as source of metal salts, use may be made of an extract of plants or yeast rich in cations. Similarly, calcium may, for example, be introduced via a milk extract.

The divalent inorganic cation content used in the compositions according to the invention depends, of course, on the form of the cation under consideration, and can be determined by means of simple routine experiments. These daily doses can in particular range from 100 pg to 5 pg, more particularly from 1 mg to 2 g, or even from 10 mg to 1.3 g.

In the compositions intended for oral administration according to the invention, the divalent inorganic cation "concentration can be adjusted so as to correspond to doses ranging from 1 to 3000 mg/day, and in particular from 10 to 2000 mg/day.

By way of illustration of the compositions and methods in accordance with the invention, mention may in particular be made of those that combine at least one magnesium and/or calcium salt, especially organic salt, and in particular chosen from magnesium gluconate, lactate and/or citrate and/or calcium gluconate, lactate and/or citrate and/or strontium nitrate, with at least one lactic acid bacterium, especially chosen from Lactobacillus sp, and more particularly Bifidobacterium lactis “CNCM I-3446".

According to the variants of the invention, the compositions can be administered topically or orally.

The compositions according to the invention may be in any of the pharmaceutical forms normally used, according to the route for use.

The carrier may be of diverse nature according to the type of composition under consideration.

Food or pharmaceutical carriers that are especially suitable include milk, yogurt, cheese, fermented milks, milk-based fermented products, ice creams, fermented cereal-based products, milk-based powders, formulas for children and infants, foods for animals, in particular pets, tablets or lozenges, liquid bacterial suspensions, oral supplements in dry form and oral supplements in liquid form.

In particular, the composition according to the invention may be a food composition for human consumption. It may in particular be complete nutritional foods, drinks, mineral waters, soups, dietetic supplements and replacement foods, nutritional bars, confectionery, milk-based or fermented milk-based products, yogurts, milk-based powders, enteral nutrition products, compositions for children and/or infants, cereal-based products or fermented cereal-based products, ice creams, chocolate, coffee, “culinary” products such as mayonnaise, tomato puree or salad dressings. The composition according to the invention may also be intended for animals.

As regards more particularly the cosmetic products, they may - be aqueous, aqueous-alcoholic or oily solutions, dispersions of the solution type or dispersions of the lotion or serum type, emulsions having a liquid or semi-liquid consistency of the milk type, suspensions or emulsions of the cream, aqueous gel or anhydrous gel type, microemulsions, microcapsules, microparticles, or vesicular dispersions of ionic and/or non- ionic type.

For ingestion, many embodiments of oral compositions and in particular of food supplements are possible. They are formulated by means of the usual methods for producing sugar- coated tablets, gelatine capsules, gels, emulsions, tablets, capsules or solutions. In particular, the active agent(s) according to the invention can be incorporated into any other forms of food supplements or of enriched foods, for example food bars, or compacted or non-compacted powders. The powders can be diluted with water, in a fizzy drink, dairy products or soya-derived products, or can be incorporated into food ‘bars.

The active agents according to the invention can be formulated with the usual excipients and constituents for such oral compositions or food supplements, i.e. in particular fatty and/or aqueous constituents, humectifying agents, thickeners, preserving agents, texturing, flavouring and/or coating agents, antioxidants, preserving agents and dyes that are usual in the food domain.

The formulating agents and excipients for oral compositions, and in particular for food supplements, are known in this domain and are not here the subject of a detailed description.

Of course, the oral compositions according to the invention can contain several other active agents.

As active agents that can be used, mention may be made of vitamins B3, B5, B6, BS, CC, E or PP, carotenoids,

curcuminoids and niacin.

In particular, use may be made of an antioxidant complex comprising vitamins C and E, and at least one carotenoid, in particular a carotenoid chosen from B-carotene, lycopene, astaxanthine, zeaxanthine and lutein, flavonoids such as catechins, hesperidin, proanthocyanidins and anthocyanins.

The composition advantageously comprises at least one prebiotic or a mixture of prebiotics. More particularly, these prebiotics can be chosen from oligosaccharides, produced from glucose, galactose, xylose, maltose, sucrose, lactose, starch, xylan, hemicellulose, inulin, gums, of the acacia type for example, or a mixture thereof. More particularly, the oligosaccharide comprises at least one fructooligosaccharide. More particularly, this prebiotic may comprise a mixture of fructooligosaccharide and of inulin.

The cosmetic and/or dermatological compositions more particularly relating to a topical application can in particular be in the form of aqueous, aqueous-alcoholic or oily solutions, of dispersions of the solution type or dispersions of the lotion or serum type, of emulsions that have a liquid or semi-liquid consistency of the milk type, obtained by dispersion of a fatty phase in an aqueous phase (O/W) or vice versa (W/O), or of suspensions or emulsions that have a soft, semi-solid or ‘solid consistency of the cream, aqueous gel or anhydrous gel type, or else of microemulsions, of microcapsules, of microparticles, or of vesicular dispersions of ionic and/or non-ionic type.

These compositions are prepared according to the usual methods.

These compositions can in particular constitute cleansing, protective, treatment or care creams for the face, for the hands, for the feet, for the major anatomical folds or for the body (for example day creams, night creams, makeup- removing creams, foundation creams, sun creams), makeup products such as fluid foundations, makeup-removing milks, protective or care milks for the body, aftersun milks, skincare lotions, gels or foams, such as cleansing or disinfecting lotions, sun lotions, artificial tanning lotions, bath compositions, deodorant compositions containing a bactericidal agent, aftershave gels or lotions, depilatory creams, or compositions for insect bites.

The compositions according to the invention may also consist of solid preparations constituting soaps or cleansing cakes.

They may also be used for the hair, in the form of aqueous, alcoholic or aqueous-alcoholic solutions, or in the form of creams, dels, emulsions or mousses, Or else in the form of aerosol compositions also containing a pressurized propellant. :

When the composition of the invention is an emulsion, the proportion of the fatty phase can range from 5 to 80% by weight, and preferably from 5 to 50% by weight, relative to the total weight of the composition. The oils, the emulsifiers and the coemulsifiers used in the composition in the form of an emulsion are chosen from those conventionally used in the cosmetics and/or dermatological field. The emulsifier and the coemulsifier may be present, in the composition, in a proportion ranging from 0.3 to 30% by weight, and preferably from 0.5 to 20% by weight, relative to the total weight of the composition.

When the composition of the invention is an oily solution or gel, the fatty phase can represent more than 90% of the total weight of the composition.

In a known manner, the cosmetic and/or dermatological composition of the invention can also contain adjuvants that are normal in the cosmetic, pharmaceutical and/or dermatological field, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preserving agents, antioxidants, solvents, fragrances, fillers, screening agents, bactericides, odour absorbers and dyestuffs. The amounts of these various adjuvants are those conventionally used in the field under consideration and are, for example, from 0.01 to 20% of the total weight of the composition. Depending on their nature, these adjuvants can be introduced into the fatty phase and/or into the aqueous phase.

As fats that can be used in the invention, mention may be made of mineral oils such as, for example, hydrogenated polyisobutene and liquid petroleum jelly, plant oils such as, for example, a liquid fraction of shea butter, sunflower oil and apricot kernel oil, animal oils such as, for example, perhydrosqualene, synthetic oils, in particular purcellin oil, isopropyl myristate and ethylhexyl palmitate, and fluoro oils such as, for example, perfluoropolyethers. Use may also be made of fatty alcohols, and fatty acids such as, for example, stearic acid and such as, for example, waxes, in particular paraffin wax, carnauba wax and beeswax. Use may also be made of silicone compounds such as silicone oils and, for example, cyclomethicone and dimethicone, and waxes, : resins and gums that contain silicone. These compounds may or may not be functionalized.

As emulsifiers that can be used in the invention, mention may, for example, be made of glyceryl stearate, polysorbate 60, the mixture of cetyl stearyl alcohol/oxyethylenated cetyl stearyl alcohol containing 33 mol of ethylene oxide sold under the name Sinnowax AO® by the company Henkel, the mixture of PEG-6/PEG-32/glycol stearate sold under the name

Tefose® 63 by the company Gattefosse, PPG-3 myristyl ether, silicone emulsifiers such as cetyl dimethicone copolyol and sorbitan monostearate or tristearate, PEG-40 stearate, and oxyethylenated (20 EO) sorbitan monostearate.

As solvents that can be used in the invention, mention may be made of lower alcohols, in particular ethanol and isopropanol, and propylene glycol.

As hydrophilic gelling agents, mention may be made of carboxylic polymers such as carbomer, acrylic copolymers such as acrylate/alkyl acrylate copolymers, polyacrylamides and in particular the mixture of polyacrylamide, Cl13-14-isoparaffin and Laureth-7 sold under the name Sepigel 305% by the company

Seppic, polysaccharides for instance cellulose derivatives such as hydroxyalkylcelluloses and in particular hydroxyl- propylcellulose and hydroxyethylcellulose, natural gums such +15 as guar, carob and xanthan gums, and clays.

As lipophilic gelling agents, mention may be made of modified clays such as bentones, metal salts of fatty acids such as aluminium stearates and hydrophobic silica, or else ethylcellulose and polyethylene.

As hydrophilic active agents, use may be made of proteins or protein hydrolysates, amino acids, polyols, in particular C: to Cio polyols such as glycerol, sorbitol, butylene glycol and polyethylene glycol, urea, allantoin, sugars and sugar derivatives, water-soluble vitamins, starch, and bacterial or plant extracts such as those of aloe vera.

As lipophilic active agents, use may be made of retinol (vitamin A) and its derivatives, tocopherol (vitamin E) and its derivatives, ceramides and essential oils.

The active agents according to the invention can also be combined with active agents intended in particular for preventing and/or treating skin conditions.

In addition, the composition of the invention can advantageously contain a spring and/or mineral water, in particular chosen from Vittel water, water from the Vichy basin and Roche Posay water.

The method of cosmetic treatment of the invention can in particular be carried out by applying the cosmetic and/or dermatological compositions as defined above, according to the normal technique for using these compositions. For example: application of creams, gels, sera, lotions, makeup- removing milks or aftersun compositions to the skin or to dry hair, application of a hair lotion to wet hair, application of shampoos, or else application of dentifrice to the gums.

The cosmetic method according to the invention can be carried out by topical administration or by oral administration, daily for example, of the combination according to the : : invention, which may, for example, be formulated in the form of gelatine capsules, gels, lotions, sugar-coated tablets, emulsions, tablets, capsules or oral ampoules, in an appropriate amount and number, according to their form, so that the active agents are administered at a rate of 5 X 10° to 103 cfu per day, in particular 10° to 10!! cfu per day, in terms of microorganisms, or at equivalent doses of partially inactivated or dead microorganisms or of microorganism fractions or of metabolites produced.

According to another embodiment, the administration is repeated until the divalent inorganic cation is administered at doses of the order of 1 to 3000 mg per day, and in particular of 10 to 2000 mg per day.

The method according to the invention can comprise a single administration. According to another embodiment, the administration is repeated, for example, 2 to 3 times daily, over a day or more, and generally over a prolonged period of at least 4 weeks, or even 4 to 15 weeks, with, where appropriate, one or more periods of interruption.

In the description and in the following examples, unless otherwise indicated, the percentages are percentages by weight and the ranges of values written as “between .. and ..” include the upper and lower limits specified. The ingredients are mixed, before they are formulated, in the order and under conditions that are readily determined by those skilled in the art.

The examples hereinafter are given by way of non-limiting illustration of the field of the invention.

Examples of compositions for topical administration

Example 1: Sensitive skin facial lotion

Lactobacillus sp. powder 5.00

Magnesium gluconate 3.00

Calcium lactate 2.00

Antioxidant 0.05

Isopropanol 40.00

Preserving agent 0.30

Water gs 100%

Example 2: Dry and sensitive skin facial care milk

Magnesium chloride 3.00

Calcium ascorbate 3.00

Lactobacillus sp. powder 5.00

Glyceryl stearate 1.00

Cetyl stearyl alcohol/oxyethylenated cetyl stearyl alcohol containing 33 mol EC (Sinnowax RO® sold by the company Henkel) 3.00

Cetyl alcohol 1.00

Dimethicone (DC 200 Fluid® sold by the company Dow Corning) 1.00

Idiquid petroleum jelly 6.00

Isopropyl myristate (Estol IMP 1514% sold by

Unichema) 3.00

Antioxidant 0.05

Glycerol 20.00

Preserving agent 0.30

Water gs 100

Example 3: Sensitive skin facial care gel

Strontium nitrate 4.00

Lactobacillus sp. 5.00

Hydroxypropylcellulose (Klucel H® sold by the company Hercules) 1.00

Vitamin E . 2.50

Antioxidant 0.05

Isopropanol 40.00

Preserving agent 0.30

Water gs 100%

Example 4: Dry and sensitive skin facial care milk

Magnesium ascorbate 3.00

Blackcurrant seed oil 4.00

Borage oil 4.00

Lactobacillus sp. 5.00

Glyceryl stearate 1.00

Cetyl stearyl alcohol/oxyethylenated cetyl stearyl alcohol containing 33 mel EO (Sinnowax AO® sold by the company Henkel) 3.00

Cetyl alcohol 1.00

Dimethicone (DC 200 Fluid® sold by the company Dow Corning) 1.00

Liquid petroleum jelly 6.00

Isopropyl myristate (Estol IPM 1514® sold by

Unichema) 3.00

Glycerol 20.00

Preserving agent 0.30

Water gs 100

Examples of compositions for oral administration

Example 5: Powder stick

Active principle

Lactobacillus sp.

Magnesium citrate

Calcium citrate

Sodium benzoate

Maltodextrin gs 30 g

One stick per day can be taken.

Example 6: Powder stick [Active principle

Lactobacillus sp. J

Bifidobacterium sp.

Calcium citrate

Sodium benzoate

Maltodextrin

One stick per day can be taken.

Example 7: Powder stick (Active principle

Lactobacillus sp.

Bifidobacterium sp.

Xanthan gum

Sodium benzoate

Maltodextrin

One stick per day can be taken.

Example 8: capsule — Tng/capsule] vitaminc | 60

One to three of these capsules can be taken per day.

S

Example 9: capsule rr Tmg/capsule vitaminc [60

One to three capsules can be taken per day.

Example 10: formulation of the sugar-coated tablet type coated tablet

Ee lied I coated tablet (Encompress™ 1 e0

Coating agent [ (shellac 1 5 [Polyvidone [6

This type of sugar-coated tablet can be taken 1 to 3 times per day.

Example 11: formulation of the sugar-coated tablet type coated tablet

Eee lela I coated tablet [Encompress™ | eo [coating agent | 0000 [Polyvinylidome 1 6

This type of sugar-coated tablet can be taken 1 to 3 times per day.

Example 12

Two oral compositions, one based only on a probiotic microorganism (B) and the other combining with this microorganism two alkaline earth metal salts (C) were tested for their effectiveness with respect to skin dryness and sensitivity, with regard to a placebo composition (A). They have the following composition:

A: Maltodextrin.

B: 1 X 10 cfu Lactobacillus paracasei CNCM I-2116 + 1 x 10'® cfu Bifidobacterium lactis (CNCM I-3446).

Cc: 1 x 10% cfu Lactobacillus paracasei CNCM I-2116 + 1 x 10% cfu Bifidobacterium lactis (CNCM I-3446) + 1 g of calcium citrate + 300 mg of magnesium citrate.

The treatment consists of the daily and oral administration of a single treatment unit for a period of eight weeks.

This study was carried out on 33 adult female individuals whose age is between 18 and 50, and who identified following a clinical evaluation (clinical score for dryness of the legs and roughness of the face) and a self-evaluation by means of a questionnaire (validated sensitive skin questionnaire) as individuals with dry and sensitive skin.

These 99 individuals were divided up into three parallel groups of 33 individuals, with two groups receiving the tested products and one group receiving the placebo.

The effect of the two supplements tested is assessed by comparison with the control “placebo” formulation. The results obtained are given in Table I below.

Table I a

D57 and significance based only on according to the versus placebo (1) probiotics (B) invention (C)

EEE

Decrease compared with Dl

Dryness of the legs -34% -42% (p = 0.2)

EE I

Decrease compared with D1

Dryness of the legs -28% (p = 0.2) -36% (p = 0.006)

EEE FN

Increase compared with D1

Urea +29% (p = 0.6) +75% (p = 0.02) (1) Analysis of the contrasts between D1 and D57, between the treatment groups and the placebo group.

Example 13

The oral composition in accordance with the invention, of the preceding example, was tested in terms of skin sensitivity in the individuals considered for the study of Example 10 (evaluation of skin sensitivity by means of a lactic acid test or stinging test).

A reduction in skin sensitivity of approximately -42% (p = 0.6) between D1 and D57 was thus observed in the treated individuals.

Claims (38)

1. Method of cosmetic treatment for preventing and/or treating sensitive and/or dry skin, comprising the oral administration of at least an effective amount of at least one probiotic microorganism chosen from Lactobacillus johnsonii (CNCM 11-1225), Lactobacillus paracasei (CNCM 11-2116), Bifidobacterium adolescentis (CNCM 1I-2168), Bifidobacterium longum (CNCM 1I-2170), Bifidobacterium lactis (CNCM I-3446) and Bifidobacterium longum (BB536), and mixtures thereof, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one divalent inorganic cation.

2. Method according to Claim 1, characterized in that it comprises the administration of at least one divalent inorganic cation in the form of a salt other than a sulphate.

3. Method of cosmetic treatment for preventing and/or treating sensitive and/or dry skin, comprising the oral or topical administration of at least an effective amount of at least one probiotic microorganism chosen from Lactobacillus johnsonii (CNCM I-1225), Lactobacillus paracasei (CNCM 11-2116), Bifidobacterium adolescentis (CNCM I-2168), Bifidobacterium longum (CNCM I-2170), Bifidobacterium lactis (CNCM I-34406) and Bifidobacterium longum (BB536), and mixtures thereof, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one divalent inorganic cation in the form of a salt other than a sulphate. Amended Sheet: 11 April 2008 J

4." Method according to Claim 1, 2 or 3, characterized in that it comprises the administration of at least one divalent inorganic cation in the form of an anhydrous or hydrated organic salt, or of a chelated complex.

5. Methed according to any one of the preceding claims, characterized in that it comprises the administration of at least two microorganisms, at least one being the probiotic type and 1s chosen from Lactobacillus johnsonii (CNCM 1-1225), Lactobacillus paracasei (CNCM 1-2116), Bifidobacterium adolescentis (CNCM I-2168), Bifidobacterium longum (CNCM I-2170), Bifidobacterium lactis (CNCM I-3446) and Bifidobacterium longrum (BB536), and mixtures thereof, and/or fractions and/or metabolites thereof, that are different.

© 6. Method according to any one of the preceding claims, characterized in that the microorganism, and/or a fraction thereof and/or a metabolite thereof, is formulated in a physiologically acceptable carrier in an amount equivalent to at least 10° cfu/g of carrier.

7. Method according to the preceding claim, characterized in that the microorganism, and/or a fraction thereof and/or a metabolite thereof, is formulated in a physiologically acceptable carrier in an amount ranging from 10° to 10%° cfu/g of carrier.

8. Method according to the preceding claim, characterized in that the microorganism, and/or a fraction thereof and/or a metabolite thereof, is formulated in a physiologically acceptable carrier in an amount ranging from 107 to 10%? cfu/g of carrier.

9. Method according to any one of the preceding claims, characterized in that it comprises the Amended Sheet: 11 April 2008 administration of at least two different divalent inorganic cations.

10. Method according to any one of the preceding claims, characterized in that said divalent inorganic cation is an alkaline earth metal.

11. Method according to any one of the preceding claims, characterized in that said alkaline earth metal is chosen from barium, calcium, magnesium, strontium and beryllium, and mixtures thereof.

12. Method according to any one of the preceding claims, characterized in that said cation is in the form of a salt chosen from carbonates, bicarbonates, gluconates, glycerophosphates, chlorides, nitrates, acetates, hydroxides, oxides, oa-hydroxy acid salts or salts of fruit acids, amino acid salts or fatty acid salts.

13. Method according to any one of the preceding claims, characterized in that said divalent inorganic cation is in the form of a salt chosen from calcium gluconates, citrates, lactates, chlorides and acetates, strontium gluconates, citrates, lactates, chlorides and acetates or magnesium gluconates, citrates, lactates, chlorides and acetates, and mixtures thereof.

14. Method according to any one of the preceding claims, characterized in that the total daily content of divalent inorganic cation ranges from 100 pg to 5 g.

15. Method according to the preceding claim, characterized in that the total daily content of divalent inorganic cation ranges from 1 mg to 2 g. Amended Sheet: 11 April 2008

16. Method according to the preceding claim, characterized in that the total daily content of divalent inorganic cation ranges from 10 mg to 1.3 g.

17. Method according to any one of the preceding claims, characterized in that it combines at least one magnesium salt and/or calcium salt with at least one lactic acid bacterium.

18. Method according to the preceding claim, characterized in that the magnesium salt and/or calcium salt are chosen from magnesium gluconate, lactate and/or citrate and/or calcium gluconate, lactate and/or citrate and strontium nitrate.

19. Method according to claim 17 or 18, characterized in that the lactic acid bacterium is chosen from Lactobacillus sp.

20. Method according to any one of the preceding claims, characterized in that the microorganism and/or a fraction thereof and/or a metabolite thereof is administered at a rate of 5 x 10° to 10° cfu per day and in that the divalent inorganic cation is administered at doses of the order of 1 to 3000 mg per day.

21. Method according to the preceding claim, characterized in that the microorganism and/or a fraction thereof and/or a metabolite thereof is administered at a rate of 10° to 10'' cfu per day.

22. Method according to claim 20 or 21, characterized in that the divalent inorganic cation is administrated at doses of the order of 10 to 2000 mg per day. Amended Sheet: 11 April 2008

23. Method according to any one of the preceding claims, characterized in that the combination is in the form of a cosmetic composition.

24. Method according to any one of Claims 1 to 22, characterized in that the combination is in the form of a food composition.

25. Use of an effective amount of at least one probiotic microorganism chosen from Lactobacillus johnsonii (CNCM 11-1225), Lactobacillus paracasei (CNCM I-2116), Bifidobacterium adolescentis (CNCM 11-2168), Bifidobacterium longum (CNCM 11-2170), Bifidobacterium lactis (CNCM I-3446) and Bifidobacterium longum (BB536), and mixtures thereof, and/or a fraction thereof and/or a metabolite thereof, in combination with at least one divalent inorganic cation, for producing a composition intended for oral administration and for treating and/or preventing sensitive skin and/or dry skin.

26. Use of an effective amount of at least one probiotic microorganism chosen from Lactobacillus johnsonii (CNCM I-1225), Lactobacillus paracasei (CNCM I-21106), Bifidobacterium adolescentis (CNCM 11-2168), Bifidobacterium longum (CNCM 1I-2170), Bifidobacterium lactis (CNCM 1I-3446) and Bifidobacterium longum (BB536), and mixtures thereof, and/or a fraction thereof and/or a metabolite thereof, in combination with at least one divalent inorganic cation in the form of a salt other than a sulphate, for producing a composition intended for treating and/or preventing sensitive skin and/or dry skin.

27. Use, as an agent intended for preventing and/or treating sensitive and/or dry skin, of at least an effective amount of at least one probiotic microorganism chosen from [Lactobacillus johnsonii (CNCM I-1225), Lactobacillus paracasei (CNCM 1I-2116), Bifidobacterium Amended Sheet: 11 April 2008

: i WO 2006/000992 PCT/IB2005/052049 k 35 adolescentis (CNCM I-2168), Bifidobacterium longum (CNCM I-2170), Bifidobacterium lactis (CNCM I-3446) and Bifidobacterium longum (BB536), and mixtures thereof, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one divalent inorganic cation in the form of a salt other than a sulphate.

28. Use according to Claim 25, 26 or 27, characterized in that said microorganism is as defined in Claims 6 to 8.

29, Use according to Claim 25, 26 or 27, characterized in that said cation is as defined in Claims 4 and 9 to 16.

30. Use according to any one of Claims 25 to 29, characterized in that the composition 1s a cosmetic composition.

31. Use according to any one of Claims 25 to 29, characterized in that the composition is a food composition.

32. Use according to claim 31, characterized in that the composition is intended for human consumption.

33. Use according to any one of Claims 25 to 29, characterized in that the composition is a dermatological composition.

34. Cosmetic and/or dermatological composition, useful for preventing and/or treating sensitive and/or dry skin, comprising, in a physiologically acceptable carrier, at least an effective amount of at least one probiotic microorganism chosen from Lactobacillus johnsonii (CNCM 11-1225), Lactobacillus paracasei (CNCM I-2116), Bifidobacterium adolescentis (CNCM 1I-2168), Amended Sheet: 11 April 2008

. i WO 2006/000992 PCT/1B2005/052049 i 36 Bifidobacterium longum (CNCM 1-2170), Bifidobacterium lactis (CNCM I-3446) and Bifidobacterium longum (BB536), and mixtures thereof, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least two alkaline earth metals in the form of anhydrous or hydrated organic salts, or of chelated complexes.

35. Composition according to the preceding claim, characterized in that said composition is useful for preventing and/or treating sensitive and/or dry skin.

36. Composition according to Claim 34 or 35, characterized in that said alkaline earth metal is as defined in any one of Claims 11 to 16.

37. Cosmetic and/or dermatological composition, useful for preventing and/or treating sensitive and/or dry skin, comprising, in a physiologically acceptable carrier, at least an effective amount of at least one probiotic microorganism chosen from Lactobacillus johnsonii (CNCM 11-1225), Lactobacillus paracasei (CNCM I-2116), Bifidobacterium adolescentis (CNCM 1I-2168), Bifidobacterium longum (CNCM 11-2170), Bifidobacterium lactis (CNCM I-3446) and Bifidobacterium longum (BB536), and mixtures thereof, and/or a fraction thereof and/or a metabolite thereof, in combination with an effective amount of at least one alkaline earth metal in the form of a salt chosen from bicarbonates, glycerophosphates, nitrates, acetates, hydroxides, o-hydroxy acid salts, or salts of fruit acids, amino acid salts, or fatty acid salts.

38. Composition according to the preceding claim, characterized in that said composition is useful for preventing and/or treating sensitive and/or dry skin. Amended Sheet: 11 April 2008