ZA200505412B - Cyclic nucleotide phosphodiestierase inhibitors, preperation and uses - Google Patents

Cyclic nucleotide phosphodiestierase inhibitors, preperation and uses Download PDF

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ZA200505412B
ZA200505412B ZA200505412A ZA200505412A ZA200505412B ZA 200505412 B ZA200505412 B ZA 200505412B ZA 200505412 A ZA200505412 A ZA 200505412A ZA 200505412 A ZA200505412 A ZA 200505412A ZA 200505412 B ZA200505412 B ZA 200505412B
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benzodiazepin
dihydro
dimethoxy
methyl
group
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ZA200505412A
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Jean-Jacques Bourguignon
Claire Lugnier
Yan Lagouge
Jean-Paul Macher
Dominique Schultz
Pierre Raboisson
Mustapha Abarghaz
Patrick Wagner
Cesare Mondadori
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Neuro 3D
Univ Pasteur
Centre Nat Rech Scient
Forenap
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Publication of ZA200505412B publication Critical patent/ZA200505412B/en

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Description

Cyclic nucleotide phosphodiesterase inhibitors, preparation and uses
The invention concerns the use of PDE2 inhibitors for treating disorders of the central and peripheral nervous system, a method for therapeutic treatment by administering to an animal said inhibitors. More specifically, the invention concerns novel benzodiazepine derivatives and their uses in therapeutics more particularly for treating pathologies involving activity of a cyclic nucleotide phosphodiesterase. The invention also concerns methods for preparing same and novel synthesis intermediates.
The compounds whose synthesis is described in the present invention are novel and possess very interesting pharmacological properties : they are inhibitors of cyclic nucleotide phosphodiesterases and more particularly of ¢GS-PDE (cGMP-Stimulated
PDEs or phosphodiesterase type 2 (PDE2) and, as such, they have very interesting therapeutic applications.
The functions of most tissues are modulated by endogenous substances such as hormones, transmitters, etc. or by exogenous substances. The biological effect of some of these substances is transmitted inside the cell by enzymatic effectors, such as adenylate cyclase or guanylate cyclase. Stimulation of said enzymes results in an elevation of intracellular levels of cyclic AMP (cAMP) or cyclic GMP (cGMP), second messengers involved in regulating many cellular activities. These cyclic nucleotides are degraded by a family of enzymes — the phosphodiesterases (PDE) — comprising at least seven groups.
One of them, PDE2, hydrolyzes both cAMP and cGMP and can be activated by cGMP. In physiological conditions PDE2 responds to high cGMP concentrations by increasing the hydrolysis of cAMP. This group is called PDE2 and is present in many tissues (adipocytes, adrenals, brain, heart, liver, lung, blood vessels, etc.). PDE2 inhibitors are capable of increasing or maintaining intracellular levels of both cAMP and cGMP and as such, find uses in the treatment of various pathologies.
The applicant has now demonstrated that certain benzodiazepines have inhibitory effects on cyclic nucleotide phosphodiesterases, particularly inhibition of PDE2. The invention also describes novel compounds exhibiting potent inhibitory activity towards isoforms, in particular a weak action on PDE3. Said selectivity can also extend to other enzymes, such as adenosine deaminase. Thus, the invention also describes novel compounds having potent inhibitory activity towards PDE2, and preferentially displaying an excellent selectivity profile on PDE2 in comparison with adenosine deaminase. Moreover, preferred compounds according to the invention have important central effects (anticonvulsant, anxiolytic, sedative, antidepressant) or peripheral effects (antitheumatismal, against auto-inflammatory diseases, against age-related liver dysfunction), and advantageously are devoid of memory impairing effects.
A first need exists to the use of at least one phosphodiesterase 2 inhibitor for preparing a pharmaceutical composition for treating pathologies of the nervous system (central and peripheral), particularly central.
More specifically, the pathologies are those due to a deregulation of the function of a neurotransmitter or a deficiency in the release of a neurotransmitter (eg., dopamine, norepinephrine, acetylcholine, etc.), in particular dopamine, such as more specifically a pathology selected in the group consisting of depression, schizophrenia, anxiety, bipolar disorder, attention deficit disorders, sleep disorders, OCD - obsessive compulsive disorder, fibromyalgia, Tourette’s syndrome, pharmacodependence (to drugs, medication, alcohol, etc.), epilepsy, Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, multiple sclerosis, obesity and Lewy body dementia.
PDE2 inhibitors can also be used according to the invention for treating other disorders involving the peripheral nervous system and peripheral organs in general, in particular pathologies of the type reduced natriuria, acute renal failure, hepatic dysfunction, acute hepatic failure, in particular due to age, and pathologies due to or involving dysfunctions of prolactin secretion, such as restless legs syndrome, rheumatismal, allergic or auto-inflammatory disorders, such as rheumatoid arthritis, rhinitis and asthma.
A particular need exists to use PDE2 inhibitors for preparing a medicament for treating central or peripheral nervous system disorders, chronic or acute, or peripheral use of said inhibitors as vasoconstrictors.
A particular need exists to use, the PDE2 inhibitors to treat anxiety, depression or schizophrenia.
AMENDED SHEET
Inhibitors of the activity or the expression of type PDE2 phosphodiesterase which are particularly useful according to the invention are compounds which have selective PDE2 inhibitory activity, that is to say, they have less inhibitory activity towards other phosphodiesterases and particularly PDE1, PDE3, PDE4 and PDES.
Some of the PDE? inhibitors are selected in particular in the scope of the invention for their selective inhibition of PDE2 relative to adenosine deaminase, meaning that they have more potent inhibitory activity for PDE2 than for adenosine deaminase.
Preferably, the PDE2 inhibitors used in the invention can be selected in the group consisting of 1,4-benzodiazepine derivatives.
In this context, the invention also describes novel compounds having potent
PDE2 inhibitory activity. More particularly, the invention thus has as object compounds represented by general formula (I)
Ro Ri z
R N
8 or
R; —=N Rs
Rs R (1) 5 Ts in which : . Z represents an oxygen, sulfur atom or a NR; group, 20 . Ry is the hydrogen atom, a (C;-C¢) alkyl group, a (C¢-C;s) aryl group or a (C;-
Cs)alkyl(Ce-C,s)aryl or (C-C)g)aryl(C,-Cs)alkyl group, . R, is a hydrogen atom, a (C;-Cs) alkyl group, a (C¢-Cis) aryl group or a (C;-
Ce)alkyl(Ce-Cg)aryl or (Ce-Cis)aryl(C;-Cy)alkyl group,
R; and R; taken together can optionally form a linear or branched hydrocarbon chain having from 2 to 6 carbon atoms, possibly containing one or several other double bonds and/or possibly interrupted by an oxygen, sulfur or nitrogen atom,
. R3 and Ry, which are the same or different, represent the hydrogen atom, a (C;-Ci2) alkyl, (C3-Cg) cycloalkyl, (Ce-Cis) aryl, (Cs-Cig)aryl(Ci-Cq)alkyl, (C,-C,2)alkyl(Ce- :
Cis)aryl group or a (Cs-Ci3) heterocycle, aromatic or not, containing 1 to 3 heteroatoms, aNO,, CF; CN, NR’R”’, SR’, OR’, COOR’, CONR’R”’ or NHCOR’R’’ group, R’ and
R’’, independently of each other, being selected in the group consisting of the hydrogen atom, a (C;-C¢) alkyl, (C3-C¢) cycloalkyl, (C¢-Cj2) aryl group, and a (Cs-Ci2) heterocycle, aromatic or not, containing 1 to 3 heteroatoms; 10. Rs represents a phenyl group substituted at least in position 3, a naphthyl group, a (Cs-
Cis) heterocycle, aromatic or not, containing 1 to 3 heteroatoms, selected in the group consisting of the pyridyl, isoquinolyl, quinolyl and piperazinyl group, provided that when Rs is a naphthyl group substituted in position 6, then the latter is not attached to the rest of the molecule in position 2, or when Rs is a pyridyl group, then it is not attached to the rest of the molecule in position 4, or when Rs is a tetrahydro 1,2,3,4- isoquinolyl group, then it is not attached to the rest of the molecule in position 2, when Rs represents a phenyl group substituted at least in position 3, said substituent being selected in the group consisting of : an alkyl, halogenoalkyl, cycloalkyl, alkenyl, alkynyl, aralkyl, aryl, heterocycle, heterocycloalkyl group, a OH, =O, NO,, NH,, CN,
CF; COR’, COOR’, (C;-Cglalkoxy, (di)(C,-Ce)alkylamino, NHCOR’, CONR’R”’ group, in which R’ and R’ are defined as hereinabove, CHO, CONH2, phenyl optionally substituted, in particular by an acetyl group, by a halogen atom (Cl), by a
CONH2 group or by a CN group, prop-1-ynyl optionally substituted, in particular by a benzyloxy or tert-butyl carbamate group, hex-1-ynyl optionally substituted, in particular by a CN or NH2 group, pentyl optionally substituted, in particular by a CONH2, hexyl, piperidinyl optionally substituted, in particular by a prop-1-ynyl, benzylaminomethyl, acetamide (CH3CONH), aminomethyl, NH2CS-, 4-phenyl-1, 3-thiazol-2-yl, -
CONHBenzyl, -COOEthyl, -CONHiPropyl, -CONH-(CH2)n-CONH?2 (n representing a whole number from 1 to 6), -CONR’R”’, with R’ and R’’, which are the same or different, representing a C1-C6 alkyl group or a hydrogen atom, -(4-benzylpyperazin-1-yl)carbonyl, -CONH-(CH2)n- phenyl (n representing a whole number from 1 to 6), imidazolyl, piperazinyl optionally substituted, in particular by a phenyl group,
. R7 and Rg, independently of each other, are selected in the group consisting of the hydrogen atom, a halogen atom or a OR group, in which Rjo represents a hydrogen atom, a (C,-Cq) alkyl, (C3-C) cycloalkyl, (Cs-C)2) aryl group, or a (Cs-C;) heterocycle, 5 aromatic or not, comprising 1 to 3 heteroatoms, at least one of the groups R; and Rs representing a OR jo group such as defined hereinabove, . Rg and Rs, independently of each other, are selected in the group consisting of the hydrogen atom, a halogen atom, an alkyl, cycloalkyl, alkenyl, alkynyl group, an aryl, aralkyl, heterocycle, heterocycloalkyl group and a OR 4 group, Ro being such as defined hereinabove, the alkyl, cycloalkyl, alkenyl, alkynyl, aralkyl, aryl, phenyl, naphthyl, heterocycle, heterocycloalkyl group or the hydrocarbon chain defined earlier being optionally substituted by one or more substituents, which are the same or different, preferably selected in the group consisting of a halogen atom, an alkyl, halogenoalkyl, cycloalkyl, alkenyl, alkynyl, aralkyl, aryl, heterocycle, heterocycloalkyl group, a OH, =0, NO,
NH, CN, CF;, COR’, COOR’, (C;-Cg)alkoxy, (di)(C,-Cg)alkylamino, NHCOR’ and
CONR’R”’ group, in which R’ and R’’ are such as defined hereinabove, the substituents also being optionally substituted, and the salts of compounds represented by formula (I), with the exception of compounds represented by formula (I) in which - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, RS is a phenyl group substituted at least in position 3 by a methoxy group, - Rl is an alkyl group or a hydrogen atom, R3 and R’3 are hydrogen atoms, R6 and RY are hydrogen atoms, RS is a phenyl group substituted only in position 3 by a chlorine or bromine atom, - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, RS is a phenyl group substituted at least in position 3 by a CH2OH group, - Rl is a hydrogen atom, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, R5 is a phenyl group substituted only in position 3 by a CF3 group,
- Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, RS is a phenyl group substituted in positions 3 and 5 by a CF3 group, - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, R7 and R8 are methoxy groups, R5 is a phenyl group substituted in position 3 by a phenyl group, - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, R7 and R8 are methoxy groups, RS is a phenyl group substituted in position 3 by a phenylethynyl group.
The invention also concerns pharmaceutical compositions comprising one or more compounds represented by general formula (I) such as defined hereinabove, and a pharmaceutically acceptable vehicle or excipient.
The invention further concerns the use of compounds represented by general formula (I) such as defined hereinabove for preparing a pharmaceutical composition intended for the inhibition of a cyclic nucleotide phosphodiesterase, in particular phosphodiesterase 2 (PDE2). More particularly, the invention concerns the use of the above compounds for treating pathologies involving a deregulation of intracellular levels of cyclic AMP and/or cyclic GMP.
In the spirit of the invention, the term “alkyl” designates a linear or branched hydrocarbon group advantageously containing from 1 to 12 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, zert-butyl, pentyl, neopentyl, n-hexyl, n- decyl, n-dodecyl, etc. C;-Cq groups are preferred. The alkyl groups may be substituted by an aryl group such as defined hereinbelow, in which case it is called an arylalkyl (or aralkyl) group. Benzyl and phenethyl are specific examples of arylalkyl groups.
The term “cycloalkyl” denotes a cyclic hydrocarbon system, which may advantageously contain from 3 to 6 carbon atoms and be mono- or poly-cyclic.
Examples include cyclopropyl and cyclohexyl groups in particular. “Aryl” groups are mono-, bi- or tri-cyclic aromatic hydrocarbon systems, preferably monocyclic or bicyclic aromatic hydrocarbon systems containing from 6 to 18 carbon atoms, even more preferably 6 carbon atoms. Examples include phenyl, naphthyl and biphenyl groups.
“Heterocycle” groups denote hydrocarbon systems, aromatic or not, containing one or more cyclic heteroatoms. Preferably they are cyclic hydrocarbon systems containing from 5 to 18 carbon atoms and one or more cyclic heteroatoms, particularly from 1 to 3 or 4 cyclic heteroatoms chosen from among N, O and S. Preferred aromatic heterocyclic groups (heteroaryls) include in particular thienyl, benzothienyl, benzofuryl, pyridyl, pyrimidinyl, pyridazinyl, isoquinolyl, quinolyl, thiazolyl, furyl, pyranyl, pyrrolyl, 2H-pyrrolyl, imidazolyl, benzymidazolyl, pyrazolyl, isothiazolyl, isoxazolyl and indolyl groups. Preferred nonaromatic heterocyclic groups include in particular the morpholino, piperidinyl, piperazinyl and pyrrolidinyl groups.
The aryl and heterocycle groups may possibly be substituted by an alkyl, alkenyl or alkynyl group. An aryl or a heterocycle substituted by an alkyl group is called an alkylaryl or alkylheterocycle group. Examples of alkylaryl groups include in particular tolyl, mesythyl and xylyl. An aryl or a heterocycle substituted by an alkeny! group is referred to as an alkenylaryl or alkenylheterocycle group. Examples of alkenylaryl groups include in particular the cinnamyl group. An aryl or a heterocycle substituted by an alkynyl group is called an alkynylaryl or alkynylheterocycle group.
The aryl and heterocycle groups may also be substituted by a group independently selected from aryl or heterocycle groups, themselves optionally substituted by one or more substituents preferably selected in the group consisting of a halogen atom and a NO,, CN, CF;, OR’, COR’, COOR’, alkoxy, NHCOR’ or
CONR’R”’ group, R’ and R’’ being such as defined hereinabove.
Specific examples of aryl and heterocycle groups substituted by an aryl or heterocycle group are the benzothienyl, benzofuryl, furylphenyl, benzyloxynaphthyl, pyridylphenyl, phenylphenyl and thienylphenyl groups. As noted, the hereinabove groups may be substituted. In this respect one example is the phenyl groups substituted by a phenyl group itself substituted by a halogen atom, a NO,, CF3, methoxy or methyl group. “Alkenyl” groups are linear or branched hydrocarbon functions containing one or more double bonds. Advantageously they contain from 2 to 6 carbon atoms and, preferably, 1 or 2 double bonds. Alkenyl groups may be substituted by an aryl group such as defined hereinabove, in which case it is called an arylalkenyl group. “Alkynyl” groups are linear or branched hydrocarbon functions containing one or more triple bonds. Advantageously they contain from 2 to 6 carbon atoms and,
preferably, 1 or 2 double bonds. Alkynyl groups may be substituted by an aryl group such as defined hereinabove, in which case it is called an arylalkynyl group. “Alkoxy” groups correspond to the alkyl and cycloalkyl groups defined hereinabove linked to the nucleus by an —O- (ether) bond. Methoxy and ethoxy groups are especially preferred. “Halogen” designates a fluorine, chlorine, bromine or iodine atom. “Heteroatom” is an atom selected from O, N and S.
More particularly, the invention has as its object compounds represented by general formula (I) hereinabove in which Rs is a phenyl group substituted at least in position 3 such as defined hereinabove. Said compounds possess inhibitory properties that are especially marked and preferential for phosphodiesterase 2.
The substituent groups may be selected, for example, in the group consisting of :
CHO, CN, CONH2, NO2, CF3, NH2, halogen atom (Cl), (C1-C6) alkyl, phenyl optionally substituted, in particular by an acetyl group, by a halogen atom (CI), by a
CONH2 group or by a CN, prop-1-ynyl optionally substituted, in particular by a benzyloxy or tert-butyl carbamate group, hex-1-ynyl optionally substituted, in particular by a CN or NH2 group, pentyl optionally substituted, in particular by a CONH2, hexyl, piperidinyl group optionally substituted, in particular by a prop-1-ynyl, benzylaminomethyl, acetamide (CH3CONH), aminomethyl, NH2CS-, 4-phenyl-1, 3- thiazol-2-yl, -CONHBenzyl, -COOEthyl, -CONHiPropyl, -CONH-(CH2)n-CONH2 group (n representing a whole number from 1 to 6), -CONR’R”’, with R’ and R”’, which are the same or different, representing a C1-C6 alkyl group or a hydrogen atom, -(4- benzylpyperazin-1-yl)carbonyl, -CONH-(CH2)n-phenyl (n representing a whole number from 1 to 6), imidazolyl, piperazinyl optionally substituted, in particular by a phenyl group.
Among compounds represented by formula (I) wherein RS is a phenyl group substituted at least in position 3, one can also cite compounds represented by formula (I) in which
RS is a phenyl group substituted in positions 3 and 4, in particular by a hydrocarbon chain possibly containing at least one heteroatom, like oxygen, such as the methylenedioxy (-O-CH2-O-) chain forming a ring with the phenyl group to which it is attached.
Another particular object of the invention is compounds represented by general formula (I) hereinabove in which RS is the 3-pyridyl, 4-isoquinolyl, piperazinyl group optionally substituted, in particular in position 4 by an aryl group, such as phenyl.
Another particular object of the invention is compounds represented by general formula (I) hereinabove in which Z represents a sulfur atom or ~NR2, preferably with
R2 forming a ring of the imidazole type with R1.
Particular compounds according to the invention are those in which : - Z is the oxygen atom and/or - Rj and Rg, independently of each other, represent a OR group in which Ryo is a (C;-Cs) alkyl group, preferably an ethyl or methyl group, advantageously methyl, and/or - Rj; and Rg both represent an ethoxy or methoxy group, advantageously methoxy, or one represents a hydrogen atom and the other an ethoxy or methoxy group, advantageously methyl, and/or - Rg and Ry, which are the same or different, represent the hydrogen atom, a halogen atom, a phenyl group, a (C;-C¢) alkyl group or a OR;o group in which Rj is a (C;-C¢) alkyl group, preferably an ethyl or methyl group, and/or - Rj and Rj’, which are the same or different, represent a hydrogen atom, and/or - Ry is a (C;-Cs) alkyl, (Cs-Ci3) aryl, such as phenyl, (Cs-Cig)aryl(C;-Cs)alkyl, such as benzyl optionally substituted, or a (C,-C;3)alkyl(Cs-C,g)aryl group.
A particular family of compounds is represented by compounds having general formula (I) such as defined hereinabove in which R3 and Rj’ represent the hydrogen atom.
Another family comprises compounds having general formula (I) in which Z is the oxygen atom, R; and Rs, independently of each other, represent a OR; group in which Ry is a (C;-Cs) alkyl group, R; represents the hydrogen atom or a (C1-Ce) alkyl group, Rg and Rg represent the hydrogen atom and Rj; and Rj’ represent the hydrogen atom.
Another family comprises compounds having general formula (I) in which Z is the oxygen atom, R; and Rg, independently of each other, represent a OR, group in which R; is a (C;-Cg) alkyl group, Re and Ry, which are the same or different, represent the hydrogen atom, a halogen atom or a (C,-Cs) alkyl group and R, represents a (C,-Ci2) alkyl, aryl or (Cs-Cis)aryl(C,-Cyg)alkyl group, optionally substituted by one or more substituents, which are the same or different, selected in the group consisting of a halogen atom, an alkyl, CF;, (C,-Cs) alkoxy group.
According to a particular aspect of the invention, the compounds represented by general formula (I) hereinabove are those in which at least one of the groups R7 and RS, advantageously both, represents a OR group where Ro represents a (C;-C¢) alkyl or (Cs-Cs) cycloalkyl group. Preferably, in the compounds represented by general formula (I) according to the invention and in the particular families mentioned hereinabove, at least one of the groups R; and Rg represents, independently of each another, a methoxy or ethoxy group, advantageously methoxy, more preferably, they both represent a methoxy or ethoxy group, advantageously methoxy.
Preferably, in the compounds represented by general formula (I) according to the invention and in the particular families mentioned hereinabove, the groups Rj; and Ry’, which are the same or different, represent a hydrogen atom or a methyl, ethyl or n- propyl group. According to a particularly advantageous variant, in the compounds represented by general formula (I) according to the invention and in the particular families mentioned hereinabove, the groups Rj and Rs’ represent a hydrogen atom.
As indicated, in the compounds represented by general formula (I) according to the invention and in the particular families mentioned hereinabove, R; advantageously represents a hydrogen atom or a (C,-C;) alkyl, (Ce-C)3) aryl (for example : phenyl), (Cs-
Cis)aryl(C,-Cs)alkyl (for example : benzyl), (C;-Ci2)alkyl(Ce-C)g)aryl group, said group optionally being substituted.
As indicated, in the compounds represented by general formula (I) according to the invention and in the particular families mentioned hereinabove, Rs is advantageously a phenyl group substituted at least in position 3.
According to a first variant of the invention, Rs is a phenyl group substituted by :
(a) one or more OR’ groups, in particular methoxy or ethoxy, or (b) a COR’ group, in particular acetyl or aldehyde, or (c) a CONR’R’’group, in particular CONH2, or (d) a CN group, or (e) a trifluoromethyl group, or 63) an alkyl group, for example methyl, or alkynyl group, for example hexynyl or propynyl, or (2) an aryl group or heterocycle, in particular a phenyl, furyl, pyridyl, piperidine, thiazole or thienyl group, said aryl or heterocycle itself optionally being substituted by one or more groups preferably selected from groups (a)-(g).
Especially preferred compounds are selected from the following compounds: 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 3a 7,8-dimethoxy-[5-(3-trifluoromethyl)phenyl]-1,3-dihydro-2 H-1,4-benzodiazepin-2-one, 3d 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1F-1,4-benzodiazepin-5-yl)-benzonitrile, 4a 3-[1-(4-chlorobenzyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4¢ 3-[1-(3,4-chlorobenzyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4d 3-[7,8-dimethoxy-1-(4-methoxybenzyl)-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4e 3-[1-(3-chlorobenzyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4f 3-{7,8-dimethoxy-2-0x0-1-[3-(trifluoromethyl)benzyl]-2,3-dihydro-1H-1,4- benzodiazepin-5-yl]-benzonitrile, 4g 3-[1-(2-chlorobenzyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4h 3-{7,8-dimethoxy-2-oxo-1-[4-(trifluoromethyl)benzyl]-2,3-dihydro-1H-1,4- benzodiazepin-5-yl]-benzonitrile, 4i
3-[7,8-dimethoxy-2-0x0-1-(2-phenylethyl)-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4j : 3-(1-ethyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 4k 3-+(7,8-dimethoxy-1-propyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 41 3-(1-benzyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 4m ethyl[5-(3-cyanophenyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-1- yllacetate, 4n 7,8-dimethoxy- 1-methyl-[ 5-(3-trifluoromethyl)phenyl]-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 4p 7,8-dimethoxy-1-ethy!-5-[3-(trifluoromethyl)phenyl]-1,3-dihydro-2H-1 4- benzodiazepin-2-one, 4q 5-[3-(trifluoromethyl)phenyl]-7,8-dimethoxy-1-n-propyl-1,3-dihydro-1,4- benzodiazepin-2-one, 4r 1-benzyl-5-[3-(trifluoromethyl)phenyl]-7,8-dimethoxy-1,3-dihydro-1,4-benzodiazepin- 2-one, 4s 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-benzamide,
Sa 3-(6-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sb 3-(7,8-dimethoxy-1-methyl-2-0x0-6-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sc 3-(9-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sd 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, 5e 3-(7,8-dimethoxy-1-propyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, 5f 3-(1-ethyl-7,8-dimethoxy-2-0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, Sg 3-(1-benzyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, 5h ethyl {5-[3-(aminocarbonyl)phenyl]-7,8-dimethoxy-2-ox0-2,3-dihydro-1H-1,4- benzodiazepin-1-yl} acetate, Si : 3-(7,8-dimethoxy-1,3-dimethyl-2-0x0-2,3-dihydro- 1H-1,4-benzodiazepin-5-yl) benzamide, 5j 3-[3-(3,4-dichlorobenzyl)-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzamide, Sk 3-(8-methoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, 51 3-(7,8-dimethoxy-1-methyl-2-0x0-9-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sm 3-(6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, Sn 3-(6,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide,
So tert-butyl-3-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1 H-1,4-benzodiazepin-5- yl)phenyl}propynylcarbamate, 6a 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4-benzodiazepin- 2-one, 6b 7,8-dimethoxy-1-methyl-5-[3-(3-piperidin-1-ylprop-1-ynyl)phenyl]-1,3-dihydro-2 H-1,4- benzodiazepin-2-one, 6¢ 6-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyllhex-5-ynenitrile, 6d 7,8-dimethoxy-5-(3’-hexylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2- one, 6e 5-[3-(3-aminopropyl)phenyl-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one trifluoroacetate, 6h 6-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyl}hexanamide, 6i 5-(4'-chloro-1,1"-biphenyl-3-yl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 6j 5-{3-[3-(benzyloxy)prop-1-ynyl]phenyl}-1-ethyl-7,8-dimethoxy-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 6k 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-3-carbonitrile, 61
3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carbonitrile, 6m 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carboxamide, 6n
3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-3-carboxamide, 60 3-[3-(3,4-dichlorobenzyl)-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl]benzonitrile, 7b 7,8-dimethoxy-1,3-dimethyl-5-(3-trifluoromethylphenyl)-1,3-dihydro-2H-1,4-
benzodiazepin-2-one, 7c 3-(7,8-dimethoxy-1,3-dimethyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 7d 5-[3-(aminomethy!)pheny!]-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1 4- benzodiazepin-2-one, 8a
N-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzyl]acetamide, 8b 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)thiobenzamide, 9a 7,8-dimethoxy-1-methyl-5-[3-(4-phenyl-1,3-thiazol-2-yl)phenyl]-1,3-dihydro-2H-1,4-
benzodiazepin-2-one, 9b 5-(3-cyanophenyl)-7,8-dimethoxy-1,3-dihydro-2H-1,4-benzodiazepin-2-thione, 10d 3-(8,9-dimethoxy-4H-imidazo[ 1,2-a][ 1,4]benzodiazepin-6-yl)benzonitrile, 11a 3-(8,9-dimethoxy-4H-imidazo[1,2-a][1,4]benzodiazepin-6-yl)benzamide, 11b 3-(7,8-dimethoxy-2-methylamino-1,3-dihydro-3H-1,4-benzodiazepin-5-yl)benzonitrile,
12a 7,8-dimethoxy-1-methyl-5-(3-pyridyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17b 7,8-dimethoxy-1-methyl-5-(3-nitrophenyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17¢ 5-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-2- benzonitrile, 17d
5-(3-acetylphenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17e 5-(4-isoquinolinyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17f 7,8-dimethoxy-5-(3-hydroxymethylphenyl)- 1-methyl-3-propyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 17h
5-(3-aminophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 17i 5-(3,4-dichlorophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17j 7,8-dimethoxy-1-methyl-5-(3-methylphenyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17k. 5-(3-formylphenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 171 5-[3-(benzylaminomethyl)phenyl]-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one hydrochloride, 17m
N-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) phenyl]acetamide, 17n 7,8-dimethoxy-1-methyl-5-(3,4-methylenedioxyphenyl)-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 170 3-(7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1 H-1,4-benzodiazepin-5-yl)benzonitrile, 22b 3-(6-bromo-7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 23b 3-(9-bromo-8-hydroxy-7-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 23d 3-(6-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1 H-1,4-benzodiazepin-5- yl)benzonitrile, 24b 3-(7,8-dimethoxy-1-methyl-2-0x0-6-phenyl-2,3-dihydro-1 H-1,4-benzodiazepin-5- yl)benzonitrile, 25b 3-(7,8-dimethoxy-1-methyl-2-0x0-9-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 25a tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-9-yl)phenyl]prop-2-ynylcarbamate,25¢
Methyl(2E)-3-[5-(cyanophenyl)-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-9-yl)phenyl]acrulate, 25d tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-6-yl)phenyl]prop-2-ynylcarbamate, 25e [9-(3-aminoethynyl)-7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin- 5-yl]benzonitrile, 25f
[6-(3-aminoethynyl)-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin- 5-yl]benzonitrile, 25g : 3-(8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28a 3-(6-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28b 3-(7-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28¢ 6-methoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28d 7-methoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28e 9-bromo-7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28f 3-(6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28g 3-(7-bromo-6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28h 3-(8-methoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 29a 3-(6,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 29b 3-(7-bromo-6,8-dimethoxy- 1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 29¢ 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) methyl benzoate, 34a 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzoic acid, 35a 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)N- isopropylbenzamide, 36a
N-benzyl-3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) benzamide, 36b N-(6-amino-6-oxohexyl)-3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzamide, 36¢ 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-N,N- dimethylbenzamide, 36d 5-{3-[(4-benzylpyperazin-1-yl)carbonyl]phenyl} 7,8-dimethoxy-1-methyl-2-0x0-2,3- dihydro-1H-1,4-benzodiazepin-2-one, 36e 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-N-(3- phenylpropyl)benzamide, 36f
Particularly preferred compounds are selected from the following compounds : 3-(1-benzyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 4m : 7,8-dimethoxy-1-methyl-[ 5-(3-trifluoromethyl)phenyt]-1,3-dihydro-2 H-1,4- benzodiazepin-2-one, 4p 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-benzamide,
Sa 3-(6-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sb tert-butyl-3-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyl]propynylcarbamate, 6a 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4-benzodiazepin- 2-one, 6b 6-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- ybDphenyl]hex-5-ynenitrile, 6d 7,8-dimethoxy-5-(3’-hexylphenyl)-1-N-methyl-1,3-dihydro-2 H-1,4-benzodiazepin-2- one, 6e 5-(4'-chloro-1,1'-biphenyl-3-yl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 6j 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carbonitrile, 6m 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carboxamide, 6n 3-(3,4-dichlorobenzyl)-1-ethyl-7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 7a 7,8-dimethoxy-1-methyl-5-[3-(4-phenyl-1,3-thiazol-2-yl)phenyl]-1,3-dihydro-2H-1 4- benzodiazepin-2-one, 9b 7,8-dimethoxy-1-methyl-5-(3-pyridyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17b
The compounds according to the invention may be in the form of salts, particularly acid or base salts, preferably compatible with pharmaceutical use. Among the pharmaceutically acceptable acids, non-limiting examples include hydrochloric,
hydrobromic, sulfuric, phosphoric, acetic, trifluoroacetic, lactic, pyruvic, malonic, succinic, glutaric, fumaric, tartaric, maleic, citric, ascorbic, methane or ethane sulfonic, camphoric acids, etc. Among the pharmaceutically acceptable bases, non-limiting examples include sodium hydroxide, potassium hydroxide, triethylamine, tert- butylamine, etc.
The invention also relates to a composition, in particular pharmaceutical, comprising a compound such as defined hereinabove, in particular in association with a pharmaceutically acceptable vehicle or excipient.
The PDE2 inhibitors, the compounds represented by formula (I) or the compositions according to the invention may be administered in different ways and in different forms. For instance, they may be administered systemically, by the oral route, by inhalation or by injection, such as for example by the intravenous, intramuscular, subcutaneous, transdermal, intra-arterial route, etc., the intravenous, intramuscular, subcutaneous, oral and inhalation routes being preferred. For injections, the compounds are generally prepared in the form of liquid suspensions, which can be injected through syringes or by infusion, for instance. In this respect, the compounds are generally dissolved in pharmaceutically compatible saline, physiologic, isotonic, buffered solutions and the like, known to those skilled in the art. For instance, the compositions may contain one or more agents or vehicles selected from among dispersives, solubilizers, stabilizers, preservatives, and the like. Agents or vehicles that may be used in the liquid and/or injectable formulations comprise in particular methylcellulose, hydroxymethylcellulose, carboxymethylcellulose, polysorbate 80, mannitol, gelatin, lactose, vegetable oils, acacia and the like.
The compounds may also be administered in the form of gels, oils, tablets, suppositories, powders, capsules, gelules, aerosols, and the like, possibly by means of pharmaceutical forms or devices allowing extended and/or delayed release. For this type of formulation, an agent such as cellulose, carbonates or starches is advantageously used.
It is understood that the injection rate and/or injected dose may be adapted by those skilled in the art according to the patient, the pathology, the mode of administration, etc. Typically, the compounds are administered at doses ranging from 0.1 pg to 100 mg/kg of body weight, more generally from 0.01 to 10 mg/kg, typically between 0.1 and 10 mg/kg. Furthermore, repeated injections may be given, as the case may be. Also, in the case of chronic treatments, delayed or sustained release systems may be advantageous.
The compounds according to the invention can act in particular on phosphodiesterase type PDE2. Thus, the inventive compounds can be (selective) inhibitors of PDE2, that is to say, they show less inhibitory activity towards the other phosphodiesterases and in particular PDE1, PDE3, PDE4 and PDE5. Some of the inventive compounds exhibit an inhibitory profile specific of PDE2, including with respect to adenosine deaminase, and, as such, also have advantageous therapeutic properties.
The PDE2 inhibitor compounds represented by formula (I) according to the invention are of particular interest in treating pathologies involving the central nervous system, in particular due to a deregulation of the function of a neurotransmitter or a deficiency in the release of a neurotransmitter (eg., dopamine, norepinephrine, acetylcholine, etc.), such as more specifically for the treatment of a pathology selected in the group consisting of depression, schizophrenia, anxiety, bipolar disorder, attention deficit disorders, sleep disorders, OCD - obsessive compulsive disorder, fibromyalgia,
Tourette’s syndrome, pharmacodependence (drugs, medications, alcohol, etc.), epilepsy,
Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, multiple sclerosis, obesity and Lewy body dementia.
The PDE?2 inhibitor compounds of the invention are particularly interesting in the treatment of other disorders involving the peripheral nervous system and peripheral organs in general, in particular pathologies of the type reduced natriuria, acute renal failure, hepatic dysfunction, acute hepatic failure, in particular due to age, and pathologies due to or involving dysfunctions of prolactin release, such as restless legs syndrome, rheumatismal, allergic or auto-inflammatory disorders, such as rheumatoid arthritis, rhinitis and asthma.
A particular object of the invention is therefore based on the use of compounds such as described hereinabove for preparing a medicament for treating chronic or acute disorders of the central or peripheral nervous system, or peripheral use of said compounds as vasoconstrictors.
The invention also concerns the use of the compounds as anxiolytic, anticonvulsant, sedative agents or for the treatment of memory or cognitive impairment, in particular mild cognitive impairment.
The invention further concerns the use of the hereinabove compounds for the : treatment of neurodegenerative diseases.
In the spirit of the invention, the term treatment designates a preventive or a curative treatment, which can be used alone or in combination with other agents or treatments. Moreover, it can be a treatment of chronic or acute disorders.
The invention also has as object the use of the hereinabove compounds for the treatment of obesity.
The preferred compounds of the invention advantageously show potent inhibitory activity towards PDE2. The preferred compounds of the invention further display an advantageous selectivity profile, in particular a weak activity on PDE3.
The inventive compounds can be prepared from commercially available products, by using a combination of chemical reactions known to those skilled in the art
Legends of figures
Figures 1 to 7 depict the synthetic routes of compounds represented by formula (I) according to the invention.
Figure 1: Synthesis of 1,4 benzodiazepinones and corresponding imidazobenzodiazepines, by Route A (Friedel Crafts reaction using a nitrile and
AICIl3/BCl; as Lewis acid), with Rjo=H, CN, Br, CFs.
Figure 2 : Synthesis of 1,4 benzodiazepinones by Route B (Friedel Crafts reaction using an acid chloride and SnCl, as Lewis acid), with Ryo? CN.
Figure 3 : Route C, via the iminochloride 16 of the benzodiazepinone.
Figure 4 : Synthesis and other substitutions of benzodiazepinones 4.
Figure 5: Regioselective halogenation of the benzodiazepinone catechol. Direct halogenation is also possible (in the presence of AcOH, NXS) in position Rg on a benzophenone of type 2 (diagram 1) which leads after ring formation to a benzodiazepinone of type (23a).
Figure 6 : Derivatives substituted on the benzodiazepinone benzo ring.
Figure 7 : Formation of substituted phenyl meta carboxamide compounds.
Figures 8 and 9 : Results of the elevated plus maze test carried out with an inventive } compound.
Figures 10 and 11 : Results of the swim test carried out with an inventive compound.
Figures 12 and 13: Results of the light/dark test carried out with an inventive compound.
Concerning the methods of preparation of compounds represented by formula (I), and according to a first method shown in Figure 1, the compounds represented by general formula (I) according to the invention can be obtained by carrying out the following steps starting from a compound represented by general formula 1. - Formation of ortho-aminobenzophenones 2 :
The Friedel Crafts reaction starting with a compound of general formula 1 ) NH,
MeO 1 in the presence of a compound of the substituted benzonitrile type, preferably in a halogenated solvent of the type C,;H4Cl,, in the presence of a mixture of Lewis acids such as AICIl3/BCl; (Friedel Crafts reaction), followed by hydrolysis of the imine formed in the presence of hydrochloric acid, which leads to a compound of formula 2 in which
Ryo represents the RS substituent groups, such as defined hereinabove, or is such as defined in Figure 1,
MeO NH,
OL
- Construction of the benzodiazepinone ring, 3 and derivatives 10-11-12
Route 1 carried out by heating the compound of general formula 2 under reflux in the presence of a-aminoacid ester hydrochloride and pyridine at a temperature comprised between 100°C and 150°C leads to formation of a compound having general formula 3. Route 2 carried out by addition of an acetyl halogenide of the type bromoacetyl bromide, followed by ring formation in the presence of ammonia gas in a hydroxylated solvent of the type methanol, leads to the compound having general formula 3 in which Rg is such as defined hereinabove.
[0]
MeO ~ es JL
Ro 3
Reaction of a compound of type 3 with the Lawesson reagent in toluene under reflux, can convert a compound represented by formula (I) in which Z is an oxygen atom to a compound represented by formula (I) in which Z is a sulfur atom and thus form a compound of type 10.
Transformation a compound represented by formula (I) in which Z is a sulfur atom to a compound represented by formula (I) in which Z represents NR; can be carried out in particular by reacting the sulfated compound 10 obtained in the previous step, in the presence of an amine of formula NH;R;3 or by a compound of formula (NH,)(R1)(CH2)2(OEt),, Ry; and R;3 representing a substituent group such as defined hereinabove. - Other substitutions and transformations of benzodiazepinones 3
Reaction in the presence of an alkyl halogenide, preferably in a solvent of the type
DMF in the presence of NaH, leads to formation of an N-alkylated compound of general formula 4 in which R; and Rg are such as defined hereinabove.
Ry 0
MeO " i
Rio 4
Optionally, transformation of a compound having formula 4 (Rj = 3-CN) to compound 5 is accomplished by oxidation of the aromatic nitrile function, by reaction with HO, and NaOH at 50°C in ethanol.
Optionally, transformation of a compound having formula 4 (Ry; = 3-Br) to comopund 6 is accomplished by palladium coupling in the presence of an aryl boronic acid, or a monosubstituted or monofunctionalized alkyne and a base K;PO4, K,COs, triethylamine according to the reaction partners. The Pd(0) or Pd(II) complex is of the type Pd(PPhs)4 or PACly, in a solvent of the type DMF, EtOH.
Optionally, transformation of a compound having formula 4 (Rj = 3-CN) to compound 8 is accomplished by reduction of the nitrile function by hydrogenation in methanol in the presence of Raney nickel.
Optionally, transformation of a compound having formula 4 to compound 7 is accomplished by alkylation on carbon 3 by reaction of a base, preferably Bulli, in a solvent of the type THF, and addition of an electrophile of the type alkyl, cycloalkyl, benzyl bromide or chloride, substituted or not.
According to a second method illustrated in Figure 2, compounds represented by general formula (I) can be prepared by a method comprising the following steps :
Reaction of a compound represented by general formula (II)
pe
Ne an in which R; and Rg are such as defined hereinabove, with an acylating agent, such as a compound of the type benzoyl chloride substituted at least in position 3, in the presence of a Lewis acid, in particular in the presence of SnCl,, in a halogenated solvent of the type CH,Cl, leads to formation of a benzophenone of formula 30
R, ges
J Ro 30 in which R; and Rg are such as defined hereinabove and Rj is a substituent group on the phenyl.
Reaction of the compound of formula 30 in the presence of CH;COOH and
HNO: at room temperature leads to formation of a nitrated compound of formula 31
R, NO, as
Roo 31
The hydrogenation reaction in the presence of a catalyst of the type Pd/C in methanol gives a compound of type 2
RO NH,
Rio 2
Carrying out route 1 or route 2 starting with a compound of type 2 leads to a compound of type 3.
According to another embodiment (Figure 3), compounds represented by general formula (I) according to the invention in which Z is an oxygen atom can be prepared from a compound represented by general formula 13.
Reaction of a compound of general formula 13 :
MeO i now © 13 in the presence of an alkyl halogenide, preferably in a solvent of the type DMF in the presence of NaH, leads to an N-alkylated compound of general formula 14 in which
R, is such as defined hereinabove
By
MeO
Pow © 14
Heating the compound represented by general formula 14 under reflux in the presence of an o-aminoacid ester hydrochloride and pyridine, followed by ring formation in acidic medium, for example in the presence of acetic acid, at a temperature comprised between 100°C and 150°C, leads to a comopund represented by general formula 15 in which R; is such as defined hereinabove.
Roo
MeO "
A
© 15
Reaction of the compound represented by general formula 15 in the presence of dimethylaniline (or dimethylaminopyridine) and phosphorus oxyhalogenide (preferably
POCIs), preferably at a temperature comprised between 80°C and 150°C in anhydrous
CHCl; medium and in a sealed tube, leads to the formation of an iminochloride compound represented by general formula 16.
R
2 fo)
MeO
Bee cl 16
Couplings with a boronic acid of general formula Rs-B(OH); in which Rs is such as defined hereinabove, in the presence of a base of the type K3PO,, K,CO; and a Pd(0) complex of the type Pd(PPhs)s, in a solvent of the type DMF, EtOH, leads to the formation of a compound represented by general formula 17.
After catalytic hydrogenation of the suitably substituted or protected nitrocatechols 18, compounds 22, which correspond to general formuia (I), are prepared according to the routes described earlier, with Rg and R; being defined as in Figure 5.
Ri—O 7 18 0 ir
CN 22
Reaction of a compound 22 in the presence of N-bromo or N-chloro or N-iodo succinimide, in a solvent of the type CH,Cl,, and an acid of the type acetic acid leads to a compound represented by general formula 23, with Rg and R; which are defined as in
Figure 5 and in this example Rg or Rg represent a halogen atom.
Ry H 0
R—O } BY
R—O =N
R, Q
Reaction of compound 23 in the presence of iodomethane, preferably in a solvent of the type DMF in the presence of NaH, leads to the formation of a compound represented by general formula 24 in which Rg or Rg represent a halogen atom.
R, | o
MeO } "
MeO =N
Ti
CN 24
Palladium couplings in the presence of an aryl boronic acid, or a monosubstituted or monofunctionalized alkyne and a base K;POs, K;CO;, triethylamine according to the reaction partners. The Pd(0) or Pd(Il) complex of the type Pd(PPh;)s or PdCl,, in a solvent of the type DMF, EtOH leads to the formation of compounds represented by general formula 25.
Compounds 29 corresponding to general formula I but with different substitutions or trisubstitutions on the benzodiazepine ring, were prepared according to a method described in Figure 1 and as illustrated in Figure 6.
According to another embodiment (Figure 7), compounds represented by general formula (I) according to the invention in which Z is an oxygen atom can be prepared from a compound 2a
Reaction of the compound 2a:
MeO NH, .
MeO
CN 2a by heating in the presence of a base of the type NaOH, KOH, preferably in a solvent of the alcoholic type such as methanol, ethanol, glycerol, leads to the formation of compound 32a
HO NH, “x
MeO
COOH 32a
Heating the compound of general formula 32a under reflux in the presence of an a-aminoacid ester hydrochloride and pyridine, at a temperature preferably comprised between 100°C and 150°C, leads to the formation of a compound 33a
Oo
Xr XL,
COOH 334
Reaction of a compound 33a in the presence of methyl iodide, preferably in a solvent of the type DMF in the presence of NaH, leads to the formation of a compound represented by general formula 34a \ 0
MeO | " or
COOMe 34a
Reaction of the compound 34a by heating in the presence of a base of the type
NaOH, KOH, preferably in a solvent of the alcoholic type such as methanol, ethanol, glycerol, leads to the formation of compound 35a : : \ ©
MeO " 0
Cl COOH 35a
Reaction of compound 35a with a primary or secondary amine, in the presence of a base of the type N-methyl morpholine, BOP in a solvent of the type DMF leads to the formation of amides represented by general formula 36, with Rs and R;; which are defined hereinabove. \ o
MeO "
Cl po
N, 6 36
Another object of the invention is based on a method of treatment of a pathology related to a disorder of the central or peripheral nervous system, in particular central, comprising administering to an animal, preferably a human, a PDE2 inhibitor compound, preferably a selective PDE2 inhibitor compound, such as described hereinabove. In particular, the pathologies are those identified hereinabove. The PDE2 inhibitors are preferably 1,4-benzodiazepine derivatives and in particular compounds represented by formula (I).
The invention is illustrated by the following examples, which are given for purposes of illustration and not by way of limitation.
EXAMPLES
EXAMPLE 1 : SYNTHESIS OF COMPOUNDS REPRESENTED BY FORMULA {M - Synthesis of benzophenones of type 2. 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile, 2a
At 0°C under an inert atmosphere, 2.0 g (13.06 mmoles) of 3,4-dimethoxyaniline dissolved in 17 ml of 1,2-dichloroethane, 2.5 g (19.51 mmoles) of isophthalonitrile, and 1.92 g (14.40 mmoles) of AICI; were added to a solution of 14.4 ml of borine tribromide (IM/CH,Cl,, 14.4 mmoles). The reaction was stirred at room temperature for 30 minutes, then the dichloromethane was evaporated. The reaction was heated under reflux for 16 hours, then cocled. 14 m! ¢f 1 M HC! at 0°C were added and the reaction was stirred at 80°C for 2 hours. After adding 50 ml of water, the reaction was extracted with 3 x 100 ml of CH,Cl,. The organic phases were dried on Na;SOs, filtered, evaporated to dryness and purified by chromatography on silica gel (EtOAc/hexane, 1:3). Yield : 61%. 'H NMR (CDCl;, 300 MHz) : d 3.66 (s, 3H, OCH3), 3.92 (s, 3H, OCH3), 6.17-6.48 (m, 3H, NH; + 1H Ar), 6.74 (s, 1H Ar), 7.56-7.91 (m, 4H Ar). (2-amino-4,5-dimethoxyphenyl)(3-bromophenyl)methanone, 2b
By replacing isophthalonitrile in example 2a by 3-bromobenzonitrile and proceeding in the same manner, the above product was obtained. Yield : 50%. '"H NMR (CDCl3, 200
MHz): d 3.67 (s, 3H, OCH3), 3.92 (s, 3H, OCH3), 6.20-6.24 (m, 3H, NH; + 1H Ar), 6.86 (s, 1H Ar), 7.29-7.37 (m, 1H Ar), 7.51-7.55 (m, 1H Ar), 7.61-7.65 (m, 1H Ar), 7.75-7.78 (m, 1H Ar). (2-amino-4,5-dimethoxyphenyl)(phenyl)methanone, 2¢
By replacing isophthalonitrile in example 2a by benzonitrile and proceeding in the same manner, the above product was obtained. Yield : 57%. 'H NMR (CDCls, 200 MHz) : d 3.66 (s, 3H, OCH3), 3.91 (s, 3H, OCHj), 6.21 (m, 3H, NH; + 1H Ar), 6.94 (s, 1H Ar), 7.45-7.64 (m, SH Ar).
(2-amino-4,5-dimethoxyphenyl)([3-(trifluoromethyl)phenyl]methanone, 2d
By replacing isophthalonitrile in example 2a by 3-(trifluoromethyl)benzonitrile and proceeding in the same manner, the above product was obtained. Yield : 60%. 'H NMR (CDCl3, 200 MHz) : d 3.66 (s, 3H, OCH3), 3.93 (s, 3H, OCH3), 6.21-6.31 (m, 3H, NH; + 1H Ar), 6.74 (s, 1H Ar), 7.57-7.83 (m, 4H Ar). (2-amino-4,5-diethoxyphenyl)(phenyl)methanone, 2e
By replacing isophthalonitrile in example 2a by benzonitrile, and 3,4-dimethoxyaniline by 3,4-diethoxyaniline and proceeding in the same manner, the above product was obtained. Yield : 35%. 'H NMR (CDCl3;, 300 MHz) : d 1.32 (t, 3H, -CH3), 1.48 (t, 3H, -
CH3), 3.85 (q, 2H, OCH;), 4.10 (q, 2H, OCH;), 6.19 (s, 1H Ar), 6.23 (s, 2H exchangeable, -NH,), 6.99 (s, 1H Ar), 7.42-7.62 (m, SH Ar). - Formation of benzodiazepinones of type 3. 3-(7,8-dimethoxy-2-o0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 3a
To a solution of 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile 2a (2.0 g, 7.09 mmoles) in dichloromethane (15 ml) at 0-5°C, bromoacetate bromide (0.76 ml, 8.72 mmoles) was added and then 10% Na;COs (8.5 ml) was added dropwise. The reaction was stirred for 1 hour at this temperature. The two phases were separated, the organic phase was washed with 10 ml of water, dried on Na,SO, filtered, evaporated to dryness (2.8 g). At 0°C with a CaCl; tube, the solid so obtained (2.8 g, 6.94 mmoles) was stirred in NH3 (7N)YMeOH (90 ml) for 3 hours then at room temperature for 1 hour. The mixture was heated under reflux for 3 hours and the precipitate filtered (1.78 g). Yield : 80%. 'H
NMR (CDCl3, 300 MHz) : d 3.75 (s, 3H, OCH3), 3.98 (s, 3H, OCH3), 4.50 (broad s, 2H;
CH,), 6.60 (s, 1H Ar), 6.65 (s, 1H Ar), 7.50-7.95 (m, 4H Ar), 9.04 (broad s, 1H, NH). 5-(3-bromophenyl)-7,8-dimethoxy-1,3-dihydro-2 H-1,4-benzodiazepin-2-one, 3b
By replacing 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile 2a in example 3a by (2- amino-4,5-dimethoxyphenyl)(3-bromophenyl)methanone 2b and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCl, 200 MHz) : d 3.75 (s, 3H, OCH3;), 3.97 (s, 3H, OCH};), 4.32 (broad s, 2H, CH;), 6.61 (s, 1H, 1H Ar), : 6.68 (s, 1H Ar), 7.22-7.30 (m, 1H Ar), 7.46-7.50 (m, 1H Ar), 7.57-7.61 (m, 1H Ar), 7.79-7.80 (m, 1H Ar), 8.83 (s, IH, NH). 7,8-dimethoxy-S-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 3c
By replacing 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile 2a in example 3a by (2- amino-4,5-dimethoxyphenyl)(phenyl)methanone 2¢ and proceeding in the same manner, the above product was obtained. Yield : 85%. 'H NMR (CDCls, 200 MHz) : d 3.72 (s, 3H, OCH3), 3.95 (s, 3H, OCHj3), 4.21 (broad s, 2H, CH3), 6.64 (s, 1H, 1H Ar), 6.70 (s, 1H Ar), 7.37-7.59 (m, SH Ar), 9.40 (s, 1H, NH). 7,8-dimethoxy-[5-(3-trifluoromethyl)phenyl]-1,3-dihydro-2 H-1,4-benzodiazepin-2- one, 3d
By replacing 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile 2a in example 3a by (2- amino-4,5-dimethoxyphenyl)([3-(trifluoromethyl)phenyljmethanone 2d and proceeding in the same manner, the above product was obtained. Yield : 80%. 'H NMR (CDCls, 300 MHz) : d 3.73 (s, 3H, OCH3), 3.98 (s, 3H, OCH3), 4.35 (broad s, 2H, CH), 6.62 (s, 1H, 1H Ar), 6.67 (s, 1H Ar), 7.50-7.55 (m, 1H Ar), 7.71-7.73 (m, 1H Ar), 7.78-7.81 (m, 1H Ar), 7.91 (m, 1H Ar), 8.67 (s, 1H, NH). 7,8-diethoxy-5S-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 3e
By replacing 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile 2a in example 3a by (2- amino-4,5-diethoxyphenyl)(phenyl)methanone 2e and proceeding in the same manner, the above product was obtained. Yield : 60%. MP : 233-236°C. '"H NMR (CDCl3, 200
MHz): d 1.39 (t, 3H, CH), 1.54 (t, 3H, CH3), 3.94 (q, 2H, OCH3), 4.18 (q, 2H, OCH,), 4.35 (s, 2H, CHy), 6.66 (s, 1H Ar), 6.74 (s, 1H Ar), 7.36-7.63 (m, SH Ar), 9.51 (s, 1H exchangeable, -NH).
- Alkylation of the nitrogen of type 4. : 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile, 4a
Iodomethane (0.42 ml, 6.72 mmoles) was added to a cold mixture of toluene (10 ml) and
Aliquat 336 (34 ml). Next, 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin- 5-yl)-benzonitrile 3a (1.13 g, 3.36 mmoles) and an aqueous solution of 50% sodium hydroxide (4 ml) were added with stirring. The reaction mixture was allowed to return to room temperature and stirred for 4 hours. The reaction was diluted with a 50:50 mixture of dichloromethane/water (200 ml). The phases were separated and the aqueous phase was extracted once with dichloromethane. The organic phases were pooled and dried on sodium sulfate, then filtered, evaporated to dryness and chromatographed. (eluent : CH,Cly/Et,0, 1:1). A white solid was obtained (1.08 g). Yield : 96%. '"H NMR (DMSO0-dg, 200 MHz) : d 3.41 (s, 3H, NCH3), 3.76 (s, 3H, OCHj3), 3.99 (s, 3H, OCH3), 4.30 (AB system, ?d = 1.0, Jag = 10 Hz, 2H, CH), 6.60 (s, 1H Ar), 6.80 (s, 1H Ar), 7.49-7.96 (m, 4H Ar). 5-(3-bromophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2 H-1,4-benzodiazepin-2- one, 4b
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4a by 5-(3-bromophenyl)-7,8-dimethoxy-1,3-dihydro-2H- 1,4-benzodiazepin-2-one 3b and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCl3, 200 MHz) : d 3.40 (s, 3H, NCH), 3.77 (s, 3H,
OCH3), 3.99 (s, 3H, OCH3), 4.30 (AB system, 7d = 1.1, Jap = 10 Hz, 2H, CH), 6.66 (s, 1H Ar), 6.78 (s, 1H Ar), 7.26 (m, 1H Ar), 7.55 (m, 2H Ar), 7.84 (m, 1H Ar). 3-[1-(4-chlorobenzyl)-7,8-dimethoxy-2-0xo-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4¢
By replacing iodomethane in example 4a by 4-chlorobenzyl bromide and proceeding in the same manner, the above product was obtained. Yield : 75%. '"H NMR (CDCls, 300
MHz) : d 3.71 (s, 3H, OCH3), 3.88 (s, 3H, OCH3), 4.40 (AB system, 2d = 1.0, Jog = 10
Hz, 2H, CH3), 5.10 (AB system, 2d = 0.8, Jag = 15 Hz, 2H, NCH,), 6.46 (s, 1H Ar), 6.82 (s, 1H Ar), 6.97-7.00 (m, 2H Ar), 7.10-7.13 (m, 2H Ar), 7.46-7.55 (m, 2H Ar), 7.73-7.79 (m, 2H Ar). 3-[1-(3,4-chlorobenzyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl]-benzonitrile, 4d
By replacing iodomethane in example 4a by 3,4-dichlorobenzyl chloride and proceeding in the same manner, the above product was obtained. Yield : 65%. '"H NMR (CDCls, 300 MHz) : d 3.73 (s, 3H, OCH3), 3.90 (s, 3H, OCHj), 4.40 (AB system, 2d = 1.0, Jag = 10 Hz, 2H, CHa), 5.10 (AB system, ?d = 0.8, Jag = 15 Hz, 2H, NCH), 6.52 (s, 1H Ar), 6.80 (s, 1H Ar), 6.93-6.95 (d, 1H Ar), 7.09 (s, 1H Ar), 7.23-7.26 (d, 1H Ar), 7.51-7.53 (t, 1H Ar), 7.64-7.67 (d, 1H Ar), 7.75-7.77 (d, 1H Ar), 7.86 (s, 1H Ar). 3-[7,8-dimethoxy-1-(4-methoxybenzyl)-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl]-benzonitrile, 4e
By replacing iodomethane in example 4a by 4-methoxybenzyl chloride and proceeding in the same manner, the above product was obtained. Yield : 60%. 'H NMR (CDCl, 300 MHz) : d 3.70 (s, 3H, OCH3), 3.74 (s, 3H, OCHj3), 4.40 (AB system, 2d = 1.0, Jag = 10 Hz, 2H, CH3), 3.90 (s, 3H, OCHj3), 5.10 (AB system, ?d = 0.9, Jag = 15 Hz, 2H,
NCH), 6.42 (s, 1H Ar), 6.65-6.68 (d, 2H Ar), 6.87 (s, 1H Ar), 6.95-6.97 (d, 2H Ar), 7.45-7.50 (t, 1H Ar), 7.62 (s, 1H Ar), 7.61-7.65 (d, 1H Ar), 7.68-7.70 (d, 1H Ar). 3-[1-(3-chlorobenzyl)-7,8-dimethoxy-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-S-yl}- benzonitrile, 4f
By replacing iodomethane in example 4a by 3-chlorobenzyl bromide and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCls, 300
MHz) : d 3.72 (s, 3H, OCHjs), 3.89 (s, 3H, OCH3), 4.40 (AB system, 2d = 1.0, Jag = 10
Hz, 2H, CH;), 5.10 (AB system, 2d = 0.9, Jag = 15 Hz, 2H, NCH_), 6.49 (s, 1H Ar), 6.81 (s, 1H Ar), 6.99-7.09 (m, 2H Ar), 7.11-7.18 (t, 1H Ar), 7.20 (m, 1H Ar), 7.48-7.54 (t, 1H Ar), 7.69-7.74 (m, 3H Ar). 3-{7,8-dimethoxy-2-oxo-1-[3-(triflucromethyl)benzyl]-2,3-dihydro-1H-1,4- benzodiazepin-5-yl]-benzonitrile, 4g
By replacing iodomethane in example 4a by 3-trifluoromethyl)benzyl chloride and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCls, 300 MHz) : d 3.72 (s, 3H, OCH3), 3.88 (s, 3H, OCHj3), 4.40 (AB system, 7d = 1.0, Jag = 10 Hz, 2H, CH), 5.20 (AB system, ? d = 0.8, Jog = 15 Hz, 2H, NCH), 6.50 (s, 1H Ar), 6.81 (s, 1H Ar), 7.29-7.31 (m, 3H Ar), 7.46-7.52 (m, 2H Ar), 7.69-7.75 (m, 3H Ar). 3-[1-(2-chlorobenzyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4h
By replacing iodomethane in example 4a by 2-chlorobenzyl bromide and proceeding in the same manner, the above product was obtained. Yield : 60%. '"H NMR (CDCls, 300
MHz) :d 3.70 (s, 3H, OCHj;), 3.92 (s, 3H, OCH3), 4.40 (AB system, 7d = 1.0, Jag = 10
Hz, 2H, CH,), 5.30 (AB system, ?d = 0.6, Jag = 15 Hz, 2H, NCH,), 6.45 (s, 1H Ar), 6.88 (s, 1H Ar), 6.95-6.96 (m, 2H Ar), 7.13-7.18 (m, 1H Ar), 7.28-7.30 (m, 1H Ar), 7.48-7.51 (m, 1H Ar), 7.54 (s, 1H Ar), 7.66-7.74 (m, 2H Ar). 3-{7,8-dimethoxy-2-o0xo0-1-[4-(trifluoromethyl)benzyl]-2,3-dihydro-1H-1,4- benzodiazepin-5-yl}-benzonitrile, 4i
By replacing iodomethane in example 4a by (4-trifluoromethyl)benzyl bromide and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCls, 300 MHz) : d 3.47 (s, 3H, OCH3), 3.71 (s, 3H, OCHj3), 4.40 (AB system, ?d = 1.0, Jag = 10 Hz, 2H, CH), 5.20 (AB system, ?d = 0.8, Jag = 15 Hz, 2H, NCH>), 6.48 (s, 1H Ar), 6.82 (s, 1H Ar), 7.17-7.20 (m, 2H Ar), 7.40-7.47 (m, 4H Ar), 7.73 (m, 1H
Ar), 7.87 (m, 1H Ar).
3-[7,8-dimethoxy-2-0x0-1-(2-phenylethyl)-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4j :
By replacing iodomethane in example 4a by benzyl 2-bromoethylbenzene and proceeding in the same manner, the above product was obtained. Yield : 65%. 'H NMR (CDCl3, 300 MHz) : d 2.88-2.94 (m, 2H, Ph-CH,), 3.74 (s, 3H, OCH3), 3.92 (s, 3H,
OCHj), 4.15 (AB system, 7d = 0.8, Jog = 15 Hz, 2H, NCH), 4.40 (AB system, 7d = 0.8, Jag = 14 Hz, 2H, CH3), 6.50 (s, 1H Ar), 6.75 (s, 1H Ar), 7.08-7.12 (m, SH Ar), 7.47-7.50 (t, 1H Ar), 7.53 (s, 1H Ar), 7.67 (d, 1H Ar), 7.72 (d, 1H Ar). 3-(1-ethyl-7,8-dimethoxy-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 4k 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-benzonitrile 3a (200 mg, 0.65 mmole) was added to a cold mixture of DMF (4 ml) and 60% NaH (29 mg, 0.71 mmole). The solution was stirred at room temperature for 30 minutes, then iodoethane (67 pL, 0.84 mmole) was added at 0°C. The solution was stirred at room temperature for 1 hour. 30 ml of a water/ice mixture was added and the aqueous phase was extracted with 3 x 30 ml! of Et;O. The organic phases were pooled, dried on sodium sulfate, filtered, evaporated to dryness and chromatographed on silica gel (eluent:
EtOAc). A white solid was obtained (110 mg). Yield : 48%. 'H NMR (CDCl, 200
MHz) : d 1.13-1.20 (t, 3H, -CH3), 3.66-3.78 (m, 1H, NCH), 3.80 (s, 3H, OCH3), 4.02 (s, 3H, OCH3), 4.29-4.40 (m, 1H, NCH), 4.34 (AB system, 2d = 1.0, Jag = 10 Hz, 2H, 25S CH2), 6.61 (s, 1H Ar), 6.90 (s, 1H Ar), 7.57-7.61 (t, 1H Ar), 7.77-7.80 (d, 1H Ar), 7.93-7.99 (m, 2H Ar). 3-(7,8-dimethoxy-1-propyl-2-oxo-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 41
By replacing iodoethane in example 4k by iodopropane and proceeding in the same manner, the above product was obtained. Yield : 49%. 'H NMR (CDCl;, 200 MHz) : d 0.75-0.82 (t, 3H, -CH3), 1.42-1.58 (m, 2H, CH;-CH3), 3.48-3.58 (m, 1H, NCH), 3.80 (s,
3H, OCH3), 3.83 (AB system, 2d = 1.0, Jag = 10 Hz, 2H, CH2), 4.02 (s, 3H, OCH3), 4.32-4.46 (m, 1H, NCH3), 4.83 (AB system, ?d = 1.0, Jag = 10 Hz, 2H, CH2), 6.61 (S, 1H Ar), 6.89 (s, 1H Ar), 7.57-7.61 (t, 1H Ar), 7.77-7.80 (d, 1H Ar), 7.94-8.00 (m, 2H
Ar). 3-(1-benzyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 4m
By replacing iodoethane in example 4k by benzyl bromide and proceeding in the same manner, the above product was obtained. Yield : 49%. '"H NMR (CDCls, 200 MHz): d 3.69 (s, 3H, OCHs3), 3.88 (s, 3H, OCH3), 4.40 (AB system, 2d = 1.0, Jog = 10 Hz, 2H,
CH2), 5.13 (AB system, ?d = 0.85, Jog = 15 Hz, 2H, CH2), 6.43 (s, 1H Ar), 6.86 (s, 1H
Ar), 7.01-7.26 (m, 4H Ar), 7.44-7.55 (m, 2H Ar), 7.66-7.71 (mm, 2H Ar).
Ethyl|5-(3-cyanophenyl)-7,8-dimethoxy-2-o0xe-2,3-dihydro-1H-1,4-benzodiazepin-1- yl]acetate, 4n
By replacing iodoethane in example 4k by ethyl bromoacetate and proceeding in the same manner, the above product was obtained. Yield : 55%. 'H NMR (CDCl;, 200
MHz): d 1.22 (t, 3H, CHj3), 3.75 (s, 3H, OCHj3), 3.94 (s, 3H, OCH3), 4.18 (m , 2H,
OCH), 4.36 (AB system, ?d = 0.93, Jag = 10 Hz, 2H, CH2), 4.51 (s, 2H, NCH), 6.58 (s, 1H Ar), 6.83 (s, 1H Ar), 7.49-7.57 (t, 1H Ar), 7.72-7.76 (d, 1H Ar), 7.92-7.97 (m, 2H Ar). 7,8-dimethoxy-1-ethyl-S-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 40
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4a by 7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-14- benzodiazepin-2-one 3¢ and iodomethane by iodoethane, and proceeding in the same manner, the above product was obtained. Yield : 95%. 'H NMR (CDCl3, 200 MHz) : d 1.11 (t, 3H CHj3), 3.58-3.70 (m, 1H NCH), 3.75 (s, 3H, OCH3), 3.97 (s, 3H, OCH3), 4.27 (AB system, 7d = 0.98, Jag = 10 Hz, 2H, CH), 4.29-4.39 (m, 1H NCH}), 6.68 (s, 1H Ar), 6.84 (s, 1H Ar), 7.39-7.45 (m, 3H Ar), 7.62-7.65 (m, 2H Ar).
7,8-dimethoxy-1-methyl-[5-(3-trifluoromethyl)phenyl}-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 4p :
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4a by 7,8-dimethoxy-[5-(3-trifluoromethyl)phenyl]-1,3- dihydro-2H-1,4-benzodiazepin-2-one 3d and proceeding in the same manner, the above product was obtained. Yield : 95%. '"H NMR (CDCl, 200 MHz) : d 3.42 (s, 3H, NCH3), 3.81 (s, 3H, OCH3), 4.00 (s, 3H, OCH3), 4.30 (AB system, ?d = 1.0, Jag = 10 Hz, 2H,
CHa), 6.65 (s, 1H Ar), 6.81 (s, 1H Ar), 7.52-7.57 (m, 1H Ar), 7.72-7.74 (m, 1H Ar), 7.88-7.91 (m, 1H Ar), 7.95 (s, 1H Ar). 7,8-dimethoxy-1-ethyl-5-[3-(trifluoromethyl)phenyl]-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 4q
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4a by 7,8-dimethoxy-[5-(3-trifluoromethyl)phenyl]-1,3- dihydro-2H-1,4-benzodiazepin-2-one 3d, and iodomethane by iodoethane, and proceeding in the same manner, the above product was obtained. Yield : 71%. 'H NMR (CDCl; 300MHz) : d 1.14 (t, 3H, -CH3), 3.76 (s, 3H, OCH3), 3.99 (s, 3H, OCHj3), 4.00 (ABX system, 7d = 0.61, Jax = Jgx = 13.9, 2H, -NCH3), 4.31 (AB system, ?d = 1.01,
Jas = 10, 2H, CH,), 6.62 (s, 1H Ar), 6.87 (s, 1H Ar), 7.51-7.57 (t, 1H Ar), 7.71-7.74 (d, 1H Ar), 7.80-7.85 (d, 1H Ar), 7.96 (s, |H Ar). 5-[3-(trifluoromethyl)phenyl]-7,8-dimethoxy-1-n-propyl-1,3-dihydro-1,4- benzodiazepin-2-one, 4r
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 3a in example 4a by 7,8-dimethoxy-5-(3-trifluoromethylphenyl)-1,3- dihydro-1,4-benzodiazepin-2-one 3d and iodomethane by bromopropane, and proceeding in the same manner, the above product was obtained. Yield : 75%. MP : 135-137°C. '"H NMR (CDCl;, 200 MHz) : d 0.74-0.82 (m, 3H, CH,CH3), 1.49-1.63 (m, 2H, CH,CHs3), 3.49-3.62 (m, 1H, CH), 3.78 (s, 3H, OCH3), 4.01 (s, 3H, OCH3), 4.34
(AB system, ?d = 1.00, Jap = 10.0, 2H, CH},), 4.31-4.42 (m, 1H, CH), 6.65 (s, 1H Ar), 7.89 (s, 1H Ar), 7.53-7.99 (m, 4H Ar). 1-benzyl-5-[3-(trifluoromethyl)phenyl]-7,8-dimethoxy-1,3-dihydro-1,4- benzodiazepin-2-one, 4s
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- ylbenzonitrile 3a in example 4a by 7,8-dimethoxy-5-(3-trifluoromethylphenyl)-1,3- dihydro-1,4-benzodiazepin-2-one 3d and iodomethane by benzyl bromide, and proceeding in the same manner, the above product was obtained. Yield : 80%. MP : 175-178°C. '"H NMR (CDCl3, 200 MHz) : d 3.71 (s, 3H, OCH), 3.90-3.98 (m, 4H,
OCH3+CH), 4.92-4.97 (m, 1H, CH), 5.19 (AB system, ?d = 0.80, Jag = 15, 2H, CH3), 6.51 (s, 1H Ar), 6.88 (s, 1H Ar), 7.08-7.76 (mm, SH Ar). - Oxidation of the nitrile function of type 5. 3-(7,8-dimethoxy-1-methyl-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzamide, 5a
Aqueous H»0; (30% m/m in water, 7.6 ml) and NaOH (0.5 M, 10 ml) were added dropwise to a solution of 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-14- benzodiazepin-S-yl)-benzonitrile 4a (7.5 g, 22.4 mmoles) in ethanol (100 ml). The mixture was stirred at 60°C for 2 hours then cooled to room temperature. A saturated
Na;S,0;3 solution (10 ml) was then added and the mixture was stirred for 15 minutes.
The ethanol was evaporated, the reaction medium was diluted with water (100 ml) and extracted with EtOAc (3 x 100 ml). The pooled organic phases were dried on Na;SQ,, filtered and evaporated to dryness. Recrystallization was in ethanol. A white solid was obtained. Yield : 75%. 'H NMR (CDCL3, 300 MHz) : O 3.42 (s, 3H, NCH3), 3.74 (s, 3H, OCH3), 3.99 (s, 3H, OCH3), 4.33 (AB system, OC = 1.0, Jag = 10 Hz, 2H, CH,), 5.89 (s, 1H, NHH), 6.39 (s, 1H, NHH), 6.65 (s, 1H Ar), 6.80 (s, 1H Ar), 7.47-7.50 (t, 1H Ar), 7.77 (d, 1H Ar), 7.95 (d, 1H Ar), 8.15 (s, 1H Ar).
3-(6-bromo-7,8-dimethoxy-1-methyl-2-0xo-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, 5b
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- ylbenzonitrile 4a in example Sa by 3-(6-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3- dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 24b and proceeding in the same manner, the above product was obtained. Yield : 65%. 'H NMR (CDCl;, 200 MHz) : d 3.39 (s, 3H, NCH3), 3.86 (s, 3H, OCHj3), 4.00 (s, 3H, OCH3), 4.30 (AB system, 7d = 0.9, Jap = 10 Hz, 2H, NCH), 6.83 (s, 1H Ar), 7.44 (t, 1H Ar), 7.63 (d, 1H Ar), 7.86 (d, 1H Ar), 8.00 (s, 1H Ar). 3-(7,8-dimethoxy-1-methyl-2-oxo0-6-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, 5c
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(7,8-dimethoxy-1-methyl-2-0x0-6-phenyl-2,3- dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 25b and proceeding in the same manner, the above product was obtained. Yield : 95%. '"H NMR (DMSO-dg, 300 MHz) : d 3.39 (s, 3H, NCH3), 3.46 (s, 3H, OCHj3), 4.02 (s, 3H, OCH3), 4.40 (AB system, 7d = 0.5, Jag = 10 Hz, 2H, NCH), 6.82-6.85 (m, 2H Ar), 7.00-7.15 (m, 4H Ar), 7.24-7.26 (m, 2H Ar), 7.45 (s, 1H Ar), 7.62-7.64 (d, 1H Ar), 10.21 (s, 2H, NH3). 3-(9-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sd
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(9-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3- dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 24d and proceeding in the same manner, the above product was obtained. Yield : 60%. 'H NMR (DMSO-ds, 200
MHz): d 3.18 (s, 3H, NCH3), 3.74 (s, 3H, OCHj3), 3.89 (s, 3H, OCH3), 4.20 (AB system, 2d = 0.8, Jap = 11 Hz, 2H, NCH;), 6.87 (s, 1H Ar), 7.46 (broad s, 1H, NH), 7.54-7.61 (t, 1H Ar), 7.85-7.89 (d, 1H Ar), 8.03-8.20 (m, 3H, 2H Ar and NH).
3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, Se
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(7,8-dimethoxy-2-ox0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)-benzonitrile 3a and proceeding in the same manner, the above product was obtained. Yield : 67%. 'H NMR (CDCl, 200 MHz) : d 3.19 (s, 3H,
OCHa), 3.44 (s, 3H, OCH»), 3.73 (s, 2H, CH), 6.14 (s, 1H Ar), 6.22 (broad s, 2H,
NH,), 6.34 (s, 1H Ar), 6.95-7.02 (t, 1H Ar), 7.20-7.24 (d, 1H Ar), 7.50-7.54 (d, 1H
Ar), 7.62 (s, 1H Ar), 9.70 (s, 1H exchangeable, NH). 3-(7,8-dimethoxy-1-propyl-2-oxo-2,3-dihydro-1H-1,4-benzodiazepin-5- yhbenzamide, 5f
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(7,8-dimethoxy-1-propyl-2-oxo-2,3-dihydro-1H- 1,4-benzodiazepin-5-yl)benzonitrile 41 and proceeding in the same manner, the above product was obtained. Yield : 50%. 'H NMR (DMSO-dg, 200 MHz) : d 0.61 (t, 3H,
CH), 1.28-1.42 (m, 2H, CH,CH3), 3.38-3.45 (m, 1H, NCH3), 3.64 (s, 3H, OCH3), 3.92 (s, 3H, OCH3), 4.16 (AB system, ?d = 0.79, Jag = 10 Hz, 2H, CH;), 4.21-4.27 (m, 1H,
NCH,), 6.68 (s, 1H Ar), 7.19 (s, 1H Ar), 7.43-7.58 (m, 2H, 1H NH, 1H Ar), 7.69-7.74 (d, 1H Ar), 8.00-8.17 (m, 3H, 1H NH;, 2H Ar). 3-(1-ethyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide,
Sg
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(7,8-dimethoxy-1-ethyl-2-oxo-2,3-dihydro-1H- 1,4-benzodiazepin-5-yl)benzonitrile 4k and proceeding in the same manner, the above product was obtained. Yield : 53%. 'H NMR (DMSO-ds, 200 MHz) : d 0.96 (t, 3H,
CHjy), 3.33-3.42 (m, 1H, NCH), 3.64 (s, 3H, OCH3), 3.92 (s, 3H, OCHzs), 4.14 (AB system, ?d = 0.79, Jag = 10 Hz, 2H, CH,), 4.21-4.28 (m, 1H, NCH;), 6.67 (s, 1H Ar), 7.16 (s, 1H Ar), 7.43 (s, 1H NH; exchangeable), 7.50-7.58 (t, 1H Ar), 7.71-7.75 (d, 1H
Ar), 8.00-8.14 (m, 2H, 1H NH, 1H Ar).
3-(1-benzyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sh :
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(1-benzyl-7,8-dimethoxy-2-o0x0-2,3-dihydro-1H- 1,4-benzodiazepin-5-yl)benzonitrile 4m and proceeding in the same manner, the above product was obtained. Yield : 38%. 'H NMR (CDCl;, 300 MHz) : d 3.68 (s, 3H, OCH3), 3.87 (s, 3H, OCHj;), 4.42 (AB system, ?d = 0.96, Jag = 10 Hz, 2H, CH), 5.16 (AB system, ?d =0.73 Jag = 15 Hz, 2H, NCH), 5.66 (s, 1H NH3), 6.17 (s, 1H NH), 6.51 (s, 1H Ar), 7.09-7.19 (m, 6H Ar), 7.46-7.49 (d, 2H Ar), 7.89 (s, 1H Ar), 7.99-8.01 (d, 1H, 1H Ar).
Ethyl{5-[3-(aminocarbonyl)phenyl]-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-1-yl}acetate, Si
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by ethyl[5-(3-cyanophenyl)-7,8-dimethoxy-2-0x0-2,3- dihydro-1H-1,4-benzodiazepin-1-yl]acetate 4n and proceeding in the same manner, the above product was obtained. Yield : 44%. 'H NMR (CDCl3, 300 MHz) : d 1.22-1.27 (t, 3H CH), 3.75 (s, 3H, OCH3), 3.96 (s, 3H, OCH3), 4.15-4.24 (m, 2H CH,-CH3), 4.38 (AB system, 7d = 0.90, J,3 = 10 Hz, 2H, CHj), 4.50-4.57 (m, 2H NCH), 5.70 (s, IH
NH), 6.65 (s, 1H NH), 6.85 (s, 1H Ar), 7.50-7.55 (t, 1H Ar), 7.80-7.82 (d, 1H Ar), 7.97-7.99 (d, 1H Ar), 8.17 (s, 1H Ar). 3-(7,8-dimethoxy-1,3-dimethyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) benzamide, Sj
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(7,8-dimethoxy-1,3-dimethyl-2-0x0-2,3-dihydro- 1H-1,4-benzodiazepin-5-yl)benzonitrile 7d and proceeding in the same manner, the above product was obtained. Yield : 44%. 'H NMR (CDCls, 200 MHz) : d 1.75-1.78 (d, 3H CH3), 3.46 (s, 3H, NCH3), 3.76-3.80 (m, 4H, CH and OCH3), 4.01 (s, 3H OCH,),
5.68 (s, 1H exchangeable NH), 7.18 (s, 1H exchangeable NH), 6.72 (s, 1H Ar), 6.82 (s, 1H Ar), 7.48-7.56 (t, 1H Ar), 7.79-7.81 (d, 1H Ar), 7.93-7.95 (d, 1H Ar), 8.15 (s, 1H
Ar). 3-[3-(3,4-dichlorobenzyl)-7,8-dimethoxy-1-methyl-2-o0xo0-2,3-dihydro-1H-1,4- benzodiazepin-S-yl]benzamide, Sk
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example Sa by 7,8-dimethoxy-1,3-dimethyl-5-(3- trifluoromethylphenyl)-1,3-dihydro-2H-1,4-benzodiazepin-2-one 7¢ and proceeding in the same manner, the above product was obtained. Yield : 58%. 'H NMR (CDCl3, 200
MHz) : d 3.42 (s, 3H, NCH3), 3.50-3.54 (m, 2H CHa), 3.71 (s, 4H, CH and OCH), 3.97 (s, 3H OCHs), 6.01 (s, 1H exchangeable NH), 6.15 (s, 1H exchangeable NH), 6.60 (s, 1H Ar), 6.77 (s, 1H Ar), 7.21-7.50 (m, 4H Ar), 7.76-7.80 (d, 1H Ar), 7.89-7.92 (d, 1H
Ar), 8.03 (s, 1H Ar). 3-(8-methoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-S-yl)benzamide, 51
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(8-methoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzonitrile 29a and proceeding in the same manner, the above product was obtained. Yield : 60%. 'H NMR (CDCl;, 300 MHz) : d 3.43 (s, 3H,
NCH,), 3.92 (s, 3H, OCHj3), 4.32 (AB system, ?d = 0.99 Jag = 10 Hz, 2H, CH_), 5.70 (s, 1H exchangeable NH), 6.28 (s, 1H exchangeable NH), 6.75-6.78 (d, 1H Ar), 6.84 (s, 1H
Ar), 7.19-7.22 (d, 1H Ar), 7.47-7.52 (t, 1H Ar), 7.76-7.78 (d, 1H Ar), 7.94-7.96 (d, 1H
Ar), 8.09 (s, 1H Ar). 3-(7,8-dimethoxy-1-methyl-2-oxo0-9-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sm
By replacing 3-(7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3-(7,8-dimethoxy-1-methyl-2-0x0-9-phenyl-2 3- dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 25a and proceeding in the same manner, the above product was obtained. Yield : 75%. 'H NMR (CDCl3, 200 MHz) : d 2.44 (s, 3H, NCH3), 3.62 (s, 3H, OCH3), 3.77 (s, 3H, OCH3),4.41 (AB system, 7d = 0.5,
Jap = 10 Hz, 2H, NCH), 6.74 (s, 1H Ar), 7.44-7.57 (m, 6H Ar), 7.88-7.98 (m, 2H Ar), 8.25 (s, 1H Ar), 10.19 (s, 2H, NH_). 3-(6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, Sn
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example Sa by 3-(6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzonitrile 28g and proceeding in the same manner, the above product was obtained. Yield : 80%. 'H NMR (DMSO-dg, 300 MHz) : d 3.42 (s, 3H,
OCH3;), 3.85 (s, 3H, OCH3),4.17 (AB system, 2d = 0.7, Jag = 11 Hz, 2H, NCH), 6.44- 6.45 (m, 2H Ar), 7.35-7.88 (m, 4H Ar), 8.03 (s, 2H, NH3), 10.39 (s, 1H, NH). 3-(6,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, So
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example Sa by 3-(6,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H- 1,4-benzodiazepin-5-yl)benzonitrile 29b and proceeding in the same manner, the above product was obtained. Yield : 73%. 'H NMR (CDCl;, 300 MHz) : d 3.37 (s, 3H,
NCHys), 3.48 (s, 3H, OCH3), 3.90 (s, 3H, OCH3),4.28 (AB system, ?d = 0.8, Jap = 9 Hz, 2H, NCH,), 6.33 (s, 2H Ar), 6.44 (s, 2H Ar), 7.39-8.01 (m, 4H Ar). - Palladium couplings of type 6.
Tert-butyl-3-[3-(7,8-dimethoxy-1-methyl-2-o0xo0-2,3-dihydro-1H-1,4-benzodiazepin- 5-yl)phenyl]propynylcarbamate, 6a
A mixture of 100 mg (0.257 mmole) of 5-(3-bromophenyl)-7,8-dimethoxy-1-methyl- 1,3-dihydro-2H-1,4-benzodiazepin-2-one 4b, 200 mg (1.3 mmoles) of tert-butyl prop-2- ynylcarbamate, 9.0 mg of Cul, 5.0 mg of PdCl, 18.0 mg of PPhs, 0.5 ml of TEA, 2 ml of
CH;CN was stirred for 20 hours in an inert atmosphere at 50°C. The mixture was evaporated to dryness and purified by chromatography on silica gel (Et20/CH,Cl,, 1:1).
Recrystallization was in Et,O/pentane. Yield : 50%. 'H NMR (CDCls;, 200 MHz) : d 1.47 (s, 9H, C(CHjs)s3), 3.41 (s, 3H, NCH3;), 3.76 (s, 3H, OCHj3), 3.99 (s, 3H, OCHa), 4.20 (AB system, ?d = 1.0, J,p = 10 Hz, 2H, NCH), 4.12-4.15 (d, 2H, CH3), 6.65 (5, 1H Ar), 6.79 (s, 1H Ar), 7.47-7.73 (m, 4H Ar). 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2 H-1,4- benzodiazepin-2-one, 6b
By replacing tert-butyl prop-2-ynylcarbamate in example 6a by 1-hexyne and proceeding in the same manner, the above product was obtained. Yield : 89%. 'H NMR (CDCl3, 200 MHz) : d 0.91-0.98 (t, 3H, CH3), 1.40-1.60 (m, 4H, 2 CH), 2.36-2.43 (t, 2H, CH,), 3.40 (s, 3H, NCH3y), 3.76 (s, 3H, OCH3s), 3.99 (s, 3H, OCH3), 4.20 (AB system, ?d = 1.0, Jag = 11 Hz, 2H, NCH), 6.67 (s, 1H Ar), 6.78 (s, 1H Ar), 7.29-7.36 (t, 1H Ar), 7.46-7.50 (d, 1H Ar), 7.54-7.58 (d, 1H Ar), 7.65 (d, 1H Ar). 7,8-dimethoxy-1-methyl-5-[3-(3-piperidin-1-ylprop-1-ynyl)phenyl]-1,3-dihydro-2 H- 1,4-benzodiazepin-2-one, 6¢
By replacing tert-butyl prop-2-ynylcarbamate in example 6a by propargyl bromide,
PdCl; by Pd(OAc),, TEA by piperidine and CH3;CN by THF, and proceeding in the same manner, the above product was obtained. Yield : 20%. '"H NMR (CDCls, 200 MHz): d 1.48 (m, 2H, CH3), 1.62-1.65 (m, 4H, 2 CH), 2.56 (m, 4H, 2 CH};), 3.41 (s, 3H, NCH3), 3.46 (s, 2H, CHy), 3.76 (s, 3H, OCHs), 3.99 (s, 3H, OCH3), 4.20 (AB system, 7d = 1.0,
Jas =11Hz, 2H, NCH,), 6.67 (s, 1H Ar), 6.78 (s, 1H Ar), 7.34-7.71 (m, 4H Ar). 6-[3-(7,8-dimethoxy-1-methyl-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyl]hex-5-ynenitrile, 6d
By replacing tert-butyl prop-2-ynylcarbamate in example 6a by 5-cyano-1-pentyne and proceeding in the same manner, the above product was obtained. Yield : 81%. 'H NMR (CDCl, 300 MHz) : d 1.94-2.01 (m, 2H, CH), 2.54-2.63 (m, 4H, 2 CH;), 3.41 (s, 3H,
NCH), 3.76 (s, 3H, OCH3), 3.99 (s, 3H, OCHj3), 4.20 (AB system, 7d = 1.0, Jag = 11
Hz, 2H, NCH), 6.66 (s, 1H Ar), 6.79 (s, 1H Ar), 7.32-7.37 (t, 1H Ar), 7.47-7.50 (d, 1H
Ar), 7.56-7.59 (d, 1H Ar), 7.69 (d, 1H Ar). . 7,8-dimethoxy-5-(3’-hexylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2-
S one, 6e
A mixture of 68 mg (0.172 mmole) of 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N- methyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one 6b, 20 mg of Pd/C 10% by weight in ml of MeOH and 2 ml of CH,Cl; was stirred under H, at atmospheric pressure for 3 10 hours. The suspension was filtered on celite, washed with 3 x 10 ml of CH,CL,/ MeOH 8:2, evaporated to dryness and purified by silica gel chromatography (CH,Cl,/Et,0, 1:1).
Yield : 65%. 'H NMR (CDCl;, 300 MHz) : d 0.88 (m, 3H, CH3), 1.31 (m, 8H, 4 CH»), 2.60-2.65 (t, 2H, CH,), 3.40 (s, 3H, NCH3), 3.74 (s, 3H, OCHj3), 3.98 (s, 3H, OCH), 4.20 (AB system, 7d = 1.0, Jag = 11 Hz, 2H, NCH3), 6.71 (s, 1H Ar), 6.78 (s, 1H Ar), 7.28-7.49 (m, 4H Ar).
Tert-butyl-3-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-
S-yD)phenyl]propylcarbamate, 6f
By replacing 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one 6b in example 6e by fert-butyl-3-[3-(7,8-dimethoxy-1-methyl-2- 0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)phenyl]propynylcarbamate 6a and proceeding in the same manner, the above product was obtained. Yield : 58%. '"H NMR (CDCl;, 200 MHz) : d 1.44 (s, 9H, C(CH3)3), 1.81 (m, 2H, CH,), 2.63-2.70 (m, 2H,
CH), 3.14-3.22 (m, 2H, CH3), 3.41 (s, 3H, NCH3), 3.75 (s, 3H, OCHj3), 3.99 (s, 3H,
OCH3), 4.30 (AB system, 7d = 1.0, Jag = 10 Hz, 2H, NCH3), 4.56 (broad s, 1H, NH), 6.70 (s, 1H Ar), 6.78 (s, 1H Ar), 7.26-7.69 (m, 4H Ar). 6-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyl]hexanenitrile, 6g
By replacing 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1 4- benzodiazepin-2-one 6b in example 6e by 6-[3-(7,8-dimethoxy-1-methyl-2-0x0-2 3-
dihydro-1H-1,4-benzodiazepin-5-yl)phenylJhex-5-ynenitrile 6d and proceeding in the same manner, the above product was obtained. Yield : 60%. 'H NMR (CDCls, 200
MHz) : d 1.44-1.70 (m, 6H, 3 CH,), 2.34 (m, 2H, CH), 2.67 (m, 2H, CH3), 3.41 (s, 3H,
NCH3), 3.75 (s, 3H, OCH3), 3.99 (s, 3H, OCHj3), 4.30 (AB system, 2d = 1.0, Jag = 10
Hz, 2H,NCH,), 6.71 (s, 1H Ar), 6.79 (s, 1H Ar), 7.28-7.53 (m, 4H Ar). 5-[3-(3-aminopropyl)phenyl-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one trifluoroacetate, 6h
A mixture of 25 mg (0.05 mmole) of tert-butyl-3-[3-(7,8-dimethoxy-1-methyl-2-oxo- 2,3-dihydro-1H-1,4-benzodiazepin-5-yl)phenyl]propylcarbamate 6f, trifluoroacetic acid (40 01, 0.52 mmole) and CH,Cl, was stirred for 3 hours under an inert atmosphere at room temperature, then evaporated to dryness. The product was crystallized in ether (13 mg). Yield : 50%. 'H NMR (DMSO-d¢, 300 MHz) : d 1.84 (m, 2H, CH>), 2.67-2.69 (m, 2H, CH), 2.72-2.81 (m, 2H, CH), 3.34 (s, 3H, NCH3), 3.68 (s, 3H, OCH3), 3.93 (s, 3H,
OCH3), 4.15 (AB system, 2d = 0.7, Jag = 11 Hz, 2H, NCH3), 6.69 (s, 1H Ar), 7.13 (s, 1H Ar), 7.40-7.60 (m, 4H Ar), 7.83 (broad s, 3H, NH;"). 6-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- ylphenyllhexanamide, 6i
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example Sa by 6-[3-(7,8-dimethoxy-1-methyl-2-o0x0-2,3-dihydro- 1H-1,4-benzodiazepin-5-yl)phenyl Jhexanenitrile 6g and proceeding in the same manner, the above product was obtained. Yield : 50%. 'H NMR (CDCl;, 200 MHz) : d 1.37 (m, 2H, CH,), 1.62 (m, 4H, 2 CH,), 2.17-2.21 (m, 2H, CH), 2.60-2.64 (m, 2H, CH), 3.40 (s, 3H, NCH3), 3.74 (s, 3H, OCH3), 3.98 (s, 3H, OCH3), 4.30 (AB system, ?d = 1.0, J 55 = 11 Hz, 2H, NCH), 5.46 (broad s, 2H, NH), 6.70 (s, 1H Ar), 6.78 (s, 1H Ar), 7.28- 7.50 (m, 4H Ar). 5-(4'-chloro-1,1'-biphenyl-3-yl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 6j
By replacing 5-chloro-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one 16a in example 17a by 5-(3-bromophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H- 1,4-benzodiazepin-2-one 4b and 3-chlorobenzene boronic acid by 4-chlorobenzene boronic acid, and proceeding in the same manner, the above product was obtained.
Yield : 27%. MP : 131°C. '"H NMR (DMSO, 300 MHz) : d 3.32 (s, 3H, NCH3), 3.63 (s, 3H, OCH3), 3.91 (s, 3H, OCH3;), 4.16 (AB system, 2d = 1.0, Jag = 10, 2H, CH), 6.74 (s, IH Ar), 7.10 (s, 1H Ar), 7.51-7.80 (m, 8H Ar). 5-{3-[3-(benzyloxy)prop-1-ynyl]phenyl}-1-ethyl-7,8-dimethoxy-1,3-dihydre-2H-1,4- benzodiazepin-2-one, 6k
By replacing tert-butyl prop-2-ynylcarbamate in example 6a by [(prop-2- ynyloxy)methyllbenzene and proceeding in the same manner, the above product was obtained. Yield: 24%. MP : °C. '"H NMR (CDCls, 200MHz) : d 1.12 (s, 3H, CH3), 3.50- 3.72 (m, 7H, 1H NCH; + 2H OCH,Ph+ 1H CH; + OCH3), 3.97 (s, 3H, OCH3), 4.24- 4.38 (m, 2H, 1H =C-CH; + 1H NCH,), 4.66 (s, 1H, =C-CH3), 4.73-4.78 (m, 1H CH3), 6.63 (s, 1H Ar), 6.84 (s. 1H Ar), 7.26-7.83 (m, 9H Ar). 3'-(7,8-dimethoxy-1-methyl-2-oxo-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-3-carbonitrile, 61
By replacing 5-chloro-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one 16a in example 17a by 5-(3-bromophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H- 1,4-benzodiazepin-2-one 4b and 3-chlorobenzene boronic acid by 3-cyanobenzene boronic acid, and proceeding in the same manner, the above product was obtained.
Yield : 54%. 'H NMR (CDCI3, 200 MHz) : d 3.43 (s, 3H, NCH3), 3.76 (s, 3H, OCH), 4.00 (s, 3H, OCH}), 4.33 (AB system, 7d = 1.00, Jag = 10, 2H, CH3), 6.72 (s, 1H Ar), 6.81 (s, 1H Ar), 7.47-8.00 (m, 8H Ar). 3'-(7,8-dimethoxy-1-methyl-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carbonitrile, 6m
By replacing 5-chloro-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one 16a in example 17a by 5-(3-bromophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H- 1,4-benzodiazepin-2-one 4b and 3-chlorobenzene . boronic acid by 4-cyanobenzene boronic acid, and proceeding in the same manner, the above product was obtained.
Yield : 42%. 'H NMR (CDCI3, 200 MHz) : d 3.42 (s, 3H, NCH3), 3.75 (s, 3H, OCH3), 4.00 (s, 3H, OCH3), 4.33 (AB system, 7d = 1.00, Jog = 10.29, 2H, CH,), 6.73 (s, |H
Ar), 6.80 (s, 1H Ar), 7.40-7.70 (m, 7H Ar), 7.97 (s, 1H Ar). 3'-(7,8-dimethoxy-1-methyl-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carboxamide, 6n
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example Sa by 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydre-1H- 1,4-benzodiazepin-5-yl)-1,1'-biphenyl-3-carbonitrile 61 and proceeding in the same manner, the above product was obtained. Yield : 44%. 'H NMR (CDCls, 300 MHz) : d 3.44 (s, 3H, NCH), 3.77 (s, 3H, OCH3), 4.01 (s, 3H, OCH3), 4.35 (AB system, 7d = 1.00, Jap = 10, 2H, CH,), 5.66 (s, 1H exchangeable NH;), 6.08 (s, 1H exchangeable
NH;), 6.76 (s, 1H Ar), 6.82 (s, 1H Ar), 7.48-7.74 (m, 5H Ar), 7.89-7.92 (d, 2H Ar), 7.99 (s, 1H Ar). 3'-(7,8-dimethoxy-1-methyl-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-S-yl)-1,1'- biphenyl-3-carboxamide, 60
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 4a in example 5a by 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H- 1,4-benzodiazepin-5-yl)-1,1'-biphenyl-4-carbonitrile 6m and proceeding in the same manner, the above product was obtained. Yield : 44%. 'H NMR (CDCl3, 300 MHz) : d 3.44 (s, 3H, NCH3), 3.77 (s, 3H, OCH3), 4.01 (s, 3H, OCH3), 4.34 (AB system, ?d = 1.00, Jag = 10, 2H, CH,), 5.65 (s, 1H exchangeable NH), 6.19 (s, 1H exchangeable
NH), 6.76 (s, 1H Ar), 6.82 (s, 1H Ar), 7.47-7.62 (m, 4H Ar), 7.71-7.80 (m, 3H Ar), 7.99 (s, 1H Ar). - Alkylation of carbon 3 of type 7.
3-(3,4-dichlorobenzyl)-1-ethyl-7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 7a : :
LDA (2M, 0.31 ml, 0.62 mmole) was placed at -78°C under argon and stirred, then 7,8- dimethoxy-1-ethyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one 40 (100 mg, 0.31 mmole) dissolved in THF (4 ml) was added dropwise. The reaction was returned to 0°C for 30 minutes, then put back at -78°C for addition of 3,4-dichlorobenzyl (81.5 mg, 0.35 mmole). The reaction was returned to room temperature and stirred overnight. It was quenched with saturated NaCl (15 ml) and extracted with 3 x 10 ml of dichloromethane.
The organic phases were pooled and washed with 30 ml of water, dried on Na;SOs, filtered, evaporated to dryness and purified by silica gel chromatography (EtOAc/Hex, 1:1) to give the above product. Yield : 53%. 'H NMR (CDCl;, 200 MHz) : d 1.03-1.11 (t, 3H, CHj3), 3.50-3.68 (m, 4H, CH, CH; and 1H NCH), 3.73 (s, 3H, OCHj3), 3.96 (s, 3H, OCHa), 6.63 (s, 1H Ar), 6.82 (s, 1H Ar), 7.19-7.55 (m, 8H Ar). 3-[3-(3,4-dichlorobenzyl)-7,8-dimethoxy-1-methyl-2-o0xo-2,3-dihydro-1H-1,4- benzodiazepin-5-yl]benzonitrile, 7b
By replacing 7,8-dimethoxy-1-ethyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one 40 in example 7a by 3-(7,8-dimethoxy-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a and proceeding in the same manner, the above product was obtained.
Yield : 53%. '"H NMR (CDCl, 200 MHz) : d 3.46 (s, 3H, NCH3), 3.50-3.57 (m, 2H, CH and 1H CH_), 3.72-3.78 (m, 4H, 1H CH; and OCHj3;), 4.01 (s, 3H OCH3;), 6.60 (s, 1H
Ar), 6.82 (s, 1H Ar), 7.21-7.26 (m, 1H Ar), 7.38-7.42 (m, 1H Ar), 7.50-7.60 (m, 2H
Ar), 7.75-7.80 (d, 1H Ar), 7.88-7.91 (m, 2H Ar). 7,8-dimethoxy-1,3-dimethyl-5-(3-trifluoromethylphenyl)-1,3-dihydro-2H-1,4- benzodiazepin-2-one, Tc
By replacing 3,4-dichlorobenzyl in example 7a by iodomethane and proceeding in the same manner, the above product was obtained. Yield : 45%. 'H NMR (CDCl3, 200
MHz) : d 1.62-1.79 (d, 3H CH3), 3.40 (s, 3H, NCHj), 3.78-3.80 (m, 4H, CH and OCH),
4.03 (s, 3H OCH3), 6.68 (s, 1H Ar), 6.84 (s, 1H Ar), 7.52-7.60 (t, 1H Ar), 7.72-7.77 (d, 1H Ar), 7.90-7.95 (m, 2H Ar). : 3-(7,8-dimethoxy-1,3-dimethyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 7d
By replacing 7,8-dimethoxy-1-ethyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one 40 in example 7a by 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a, and 3,4-dichlorobenzyl by iodomethane, and proceeding in the same manner, the above product was obtained. Yield : 53%. 'H NMR (CDCl, 200 MHz) : d 1.72-1.75 (d, 3H CHa), 3.42 (s, 3H, NCH3), 3.75-3.77 (m, 4H, CH and OCHj3), 3.99 (s, 3H OCHs), 6.61 (s, 1H Ar), 6.80 (s, 1H Ar), 7.48-7.56 (t, 1H Ar), 7.71-7.75 (d, 1H
Ar), 7.90-7.97 (m, 2H Ar). - Reduction of the nitrile of type 8. 5-[3-(aminomethyl)phenyl]-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 8a 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-benzamide 5a (100 mg, 0.30 mmole), Raney nickel (1 spatula tip), ammonia (30%, 1 ml) and methanol (10 ml) were placed overnight under hydrogen pressure, then filtered on celite and washed with 3 x 25 ml of methanol and evaporated. The residue was taken up in 20 ml of CH;Cl,. The organic phase was washed with 3 x 20 ml of 30% ammonia and 1 x 20 ml of water. The organic phase was dried on Na;SQOq,, filtered and evaporated to dryness to give the above product. Yield : 77%. 'H NMR (CDCl;, 200 MHz) : d 1.28 (s, 2H exchangeable NH3), 1.79 (s, 2H CH), 3.42 (s, 3H, NCH3), 3.76 (s, 3H, OCHj3), 3.94 (s, 2H CH), 4.00 (s, 3H OCH3), 4.31 (AB system, ?d = 0.98, Jag = 10, 2H, CH,), 6.72 (s, 1H Ar), 6.81 (s, 1H Ar), 7.36-7.56 (m, 3H Ar), 7.71 (s, 1H Ar).
N-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzyl]acetamide, 8b
5-[3-(aminomethyl)phenyl]-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one 8a (50 mg, 0.15 mmole), acetic anhydride (16 pl, 0.18 mmole), pyridine (29.6 pl, 0.37 mmole), and 3 ml of CH,Cl, were stirred overnight, evaporated to dryness and purified by chromatography on silica gel (CH,Cl,/MeOH, 9:1) to give the above product. Yield : 77%. 'H NMR (CDCl, 200 MHz) : d 2.00 (s, 3H CH), 3.38 (s, 3H, NCH3), 3.76 (s, 3H, OCHj3), 3.94 (s, 3H OCH3), 4.25 (AB system, 2d = 0.98, Ja = 10, 2H, CH), 4.43 (s, 2H CH), 5.99 (s, 1H exchangeable NH), 6.65 (s, 1H Ar), 6.77 (s, 1H Ar), 7.26-7.40 (m, 3H Ar), 7.63 (s, 1H Ar). - Synthesis of thiobenzamides of type 9 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- ylthiobenzamide, 9a
A mixture of S00 mg (1.41 mmoles) of 3-(7,8-dimethoxy-1-methyl-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzamide Sa, 285 mg (0.71 mmole) of Lawesson reagent in 30 ml of toluene was heated overnight at 90°C. 150 ml of H,O were added and the mixture was extracted with 4 x 100 ml of EtOAc, dried on MgSO, the EtOAc was evaporated and the product was purified by silica gel chromatography (EtOAc/CH,Cl, /EtOH, 5:4:1).
Yield: 70%. '"H NMR (CDCl;, 300 MHz) : d 3.41 (s, 3H, NCH3), 3.77-3.84 (m, 4H, 1H
CH; + OCH3), 3.99 (s, 3H, OCH3), 4.81 (m, 1H, CH), 6.68 (s, 1H Ar), 6.79 (s, 1H Ar), 7.40-8.20 (m, 6H, NH, + 4H Ar). Mass : (M+H)" = 370.09. 7,8-dimethoxy-1-methyl-5-[3-(4-phenyl-1,3-thiazol-2-yl)phenyl]-1,3-dihydro-2H- 1,4-benzodiazepin-2-one, 9b
A mixture of 20 mg (0.05 mmole) of 3-(7,8-dimethoxy-1,3-dihydro-2H-14- benzodiazepin-5-yl)thiobenzamide 9a, 12 mg (0.06 mmole) of bromoacetophenone in 3 ml of EtOH was heated overnight at 100°C. 50 ml of H,O were added and the mixture was extracted with 4 x 50 ml of EtOAc, dried on MgSO, the EtOAc was evaporated and the product was purified by silica gel chromatography (EtOAc/Hex, 1:1). Yield : 90%. 'H NMR (CDCl;, 300 MHz) : d 3.45 (s, 3H, NCH3), 3.77 (s, 3H, OCH3), 4.01 (s, 3H,
OCH3), 4.36 (AB system, 7d = 1,02, Jag = 10.5, 2H, CH), 6.76 (s, IH Ar), 6.82 (s, IH
Ar), 7.44-8.00 (m, 9H Ar), 8.34 (s, 1H thiazol). Mass : (M+H)" = 470.14. - Synthesis of benzodiazepine-thiones of type 10 7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepine-2-thione, 10a 7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one 3c (400 mg, 1.35 mmoles), 600 mg of Lawesson reagent and 70 ml of anhydrous toluene were refluxed overnight, then evaporated to dryness and purified by chromatography on silica gel (EtOAc) to give the above product. Yield : 85%. 'H NMR (CDCl;, 200 MHz) : d 3.68 (s, 3H, OCHj;), 3.98 (s, 3H OCH3), 4.77 (s, 2H, CH3), 6.46 (s, 1H Ar), 7.20 (s, 1H Ar), 7.36-7.40 (m, 2H Ar), 7.54-7.60 (m, 3H Ar), 13.02 (s, 1H exchangeable NH). 7,8-diethoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepine-2-thione, 10b
By replacing 7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one 3c¢ in example 11a by 7,8-diethoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one 3e and proceeding in the same manner, the above product was obtained. Yield : 53%. '"H NMR (CDCls, 300 MHz) : d 1.33-1.38 (t, 3H, CHj3), 1.50-1.54 (t, 3H, CH3), 3.83-3.90 (q, 2H,
CH;), 4.15-4.22 (q, 2H, CH), 4.75 (s, 1H exchangeable NH), 6.47 (s, 1H Ar), 7.12 (s, 1H Ar), 7.36-7.39 (m, 2H Ar), 7.48-7.62 (m, 3H Ar). 1-ethyl-7,8-dimethoxy-S-phenyl-1,3-dihydro-2H-1,4-benzodiazepine-2-thione, 10¢
By replacing 3-(7,8-dimethoxy-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4a by 7,8-diethoxy-5-phenyl-1,3-dihydro-2H-1,4- benzodiazepine-2-thione 11b and iodomethane by iodoethane, and proceeding in the same manner, the above product was obtained. Yield: 75%. 'H NMR (CDCl, 300
MHz): d1.16-1.21 (t, 3H CHj3), 3.76 (s, 3H, OCH3), 3.95-4.02 (m, 4H, OCHj3 and 1H
NCH), 4.76 (AB system, ?d = 1.22, Jap = 10 Hz, 2H, CH), 5.05-5.12 (m, 1H NCH,), 6.68 (s, 1H Ar), 6.91 (s, 1H Ar), 7.42-7.48 (m, 3H Ar), 7.65-7.68 (m, 2H Ar).
5-(3-cyanophenyl)-7,8-dimethoxy-1,3-dihydro-2H-1,4-benzodiazepin-2-thione, 10d
A mixture of 200 mg (0.62 mmole) of 5-(3-cyanophenyl)-7,8-dimethoxy-1,3-dihydro- 2H-1,4-benzodiazepin-2-one 3a, 150 mg (0.34 mmole) of P,Ss in 4 ml of pyridine was heated under reflux for 45 minutes, then cooled to 0°C. 100 ml of saturated NaCl was then added to the suspension, which was filtered, rinsed with cold water, vacuum dried, triturated in Et,0, filtered and dried. Yield : 76%. '"H NMR (CDCl;, 300 MHz) : d 3.76 (s, 3H, OCHj;), 3.99 (s, 3H, OCHj;), 4.78 (m, 2H, CH), 6.63 (s, 1H Ar), 6.68 (s, 1H
Ar), 7.50-7.92 (m, 3H, 4H Ar), 9.84 (s, 1H exchangeable, NH ). Mass : (M+H)" = 338.10. - Nucleophilic substitution reaction (type 11 and 12) of amines on a thione. 3-(8,9-dimethoxy-4H-imidazo[1,2-a][1,4]benzodiazepin-6-yl)benzonitrile, 11a
A mixture of 100 mg (0.30 mmole) of 5-(3-cyanophenyl)-7,8-dimethoxy-1,3-dihydro- 2H-1,4-benzodiazepin-2-thione 10d, 40 mg (0.30 mmole) of aminoacetaldiethylacetal and 3 mg (0.015mmole) of paratoluenesulfonic acid monohydrate in 5 ml of butanol was heated overnight under reflux. 55 mg (0.29 mmole) of paratoluenesulfonic acid monohydrate were added and the mixture was heated under reflux for 6 h. The butanol was evaporated to 2/3, 100 ml of ice HO was added and the solution was basified to pH 8.9 with IN NaOH, then extracted with 3 x 100 ml of EtOAc and dried on MgSQO,. The
EtOAc was evaporated and the product was purified by silica chromatography (EtOAc).
Yield : 55%. '"H NMR (CDCls, 200 MHz) : d 3.77 (s, 3H, OCHj;), 4.02 (m, 4H, HCH, + OCHj3), 5.33 (m, 1HCH,), 6.68 (s, 1H Ar), 6.99 (s, 1H Ar), 7.13 (d, J)» = 1.22, 1H- imidazol), 7.36 (d, J; = 1.22, 1H-imidazol), 7.48-7.84 (m, 3H, 4H Ar). 3-(8,9-dimethoxy-4H-imidazo[1,2-a][1,4]benzodiazepin-6-yl)benzamide, 11b
By replacing 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) benzonitrile 4a in example Sa by 3-(8,9-dimethoxy-4H-imidazo[1,2- a][1,4]benzodiazepin-6-yl)benzonitrile 11a and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCls, 300 MHz) : d 3.76 (s, 3H,
OCH3;), 4.03 (m, 4H, 1HCH; + OCH3), 5.33 (m, 1HCH,), 6.53 (s, 1H Ar), 6.75 (s, lH
Ar), 6.99 (s, | H-imidazol), 7.27 (s, | H-imidazol), 7.42-8.09 (m, 3H, 4H Ar). 3-(7,8-dimethoxy-2-methylamino-1,3-dihydro-3H-1,4-benzodiazepin-5- yl)benzonitrile, 12a 1.5 ml of 2N methylamine in THF were added to a solution of 100 mg (0.30 mmole) of 5-(3-cyanophenyl)-7,8-dimethoxy-1,3-dihydro-2H-1,4-benzodiazepin-2-thione 10d in 3 ml of EtOH and 0.5 ml of DMSO, then heated overnight at 110°C. 100 ml of water H,O were added and the mixture was extracted with 3 x 100 ml of CH,Cl,, dried on MgSOa.
The CHCl, was evaporated and the product was purified by chromatography on silica gel (EtOAc; EtOAc/CH,Cl,/EtOH, 5:4:1). Yield : 30%. '"H NMR (DMSO, 300 MHz) : d 2.96 (s, 3H, NCH3), 3.59-3.71 (m, 4H, 1HCH; + OCH3), 3.98 (s, 3H, OCH3), 4.54 (m, 1HCHy,), 4.89 (broad s, 1H exchangeable, NH), 6.54 (s, 1H Ar), 6.81 (s, 1H Ar), 7.70- 7.80 (m, 4H Ar). Mass : (M+H)" = 335.1. - Synthesis of the iminochloride of type 16. 6,7-dimethoxy-2H-3,1-benzoxazine-2,4(1H)-dione, 13a 2-amino-4,5-dimethoxybenzoic acid (25 g, 0.13 mole) was added to THF (400 ml), then benzyl chloroformate (54 ml, 0.38 mole) was added with very vigorous stirring. The mixture was refluxed overnight, evaporated to dryness and the residue was vacuum evaporated. Ether (425 ml) was poured on the residue, PBr3 (11.88 ml, 0.13 mole) was added and the mixture was refluxed for 48 h. The reaction mixture was filtered and washed with 3 x 150 ml of ether. The residue was taken up in ether and stirred for 1 h, then filtered, washed and dried. The reaction produced 27 g of the above product in the form of a white powder. Yield : 96%. '"H NMR (DMSO-dg, 200 MHz) : d 3.82 (s, 3H,
OCH3), 3.88 (s, 3H, OCHj3), 6.66 (s, 1H Ar), 7.27 (s, 1H Ar), 11.58 (s, 1H exchangeable NH). 6,7-dimethoxy-1-methyl-1,2-dihydro-4H-3,1-benzoxazine-2,4-dione, 14a
Under an inert atmosphere, 134 mg (3.37 mmoles) of 60 % NaH in oil were added to a solution of 500 mg (3.06 mmoles) of 6,7-dimethoxy-1,2-dihydro-4H-3,1-benzoxazine- 2,4-dione 13a in 6 ml of anhydrous DMF. After 10 min at room temperature, 219 ul : (3.52 mmoles) of Mel were added dropwise. The reaction was left at room temperature for 3 h, then 40 ml of a water-ice mixture were added. The precipitate was filtered and washed with 2 x 1 ml of EtOH and 3 ml of Et20. The reaction produced 320 mg of the above product in the form of a white powder. Yield : 59%. '"H NMR (CDCI3, 300
MHz) : d 3.31 (s, 3H, -CH3), 3.82 (s, 3H, OCH3), 3.96 (s, 3H, OCH3), 6.85 (s, 1H Ar), 7.32 (s, 1H Ar). 7,8-dimethoxy-1-methyl-3,4-dihydro-1H-1,4-benzodiazepine-2,5-dione, 15a
A mixture of 320 mg (1.35 mmoles) of 6,7-dimethoxy-1-methyl-1,2-dihydro-4H-3,1- benzoxazine-2,4-dione 14a, 452 mg (3.24 mmoles) of methyl glycinate hydrochloride in 4 ml of pyridine was heated under reflux for 6 h. 3 ml of AcOH were added and the reaction was heated at 130°C for 12 h. After evaporation to dryness, 10 ml of a water/ice mixture were added. The mixture was allowed to crystallize for 30 minutes at 0°C, then filtered and washed with 2 x 2 ml of water, 2 x 1 ml of EtOH and 2 x 5 ml of Et20. Recrystallization was in EtOH. The reaction produced 240 mg of the above product as colorless crystals. Yield : 71%. MP : 260-263°C. 'H NMR (CDCI13, 300
MHz) : d 3.42 (s, 3H, NCH3), 3.75-3.92 (m, 2H, CH), 3.98 (s, 6H, 2 x OCH3), 6.39 (s, 1H exchangeable, NH), 6.69 (s, 1H Ar), 7.37 (s, 1H Ar). 5-chloro-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 16a
A solution of 100 mg (0.40 mmole) of 7,8-dimethoxy-1-methyl-3,4-dihydro-1H-14- benzodiazepine-2,5-dione 15a, 280 pl of dimethylaniline, 800 pl of POCI3, in 10 ml of anhydrous CHCI3 was heated in a sealed tube at 125°C for % hour, then cooled to room temperature. 3 g of silica and 5 ml of CH2CI2 were added. At 0°C, 1 ml of triethylamine was added. After evaporation to dryness, purification was by chromatography (EtOAc/Hexane 1:1, then EtOAc). The purified fraction was pulverized in 1 ml of Et20, filtered and washed with 2 x 2 ml of pentane. The reaction produced 93 mg of the above product in the form of a white powder. Yield : 87%. 'H NMR (CDCI3, 200 MHz) : d 3.42 (s, 3H, NCH3), 3.77 (broad s, 1H of CH,), 3.99 (s, 3H, OCH3), 4.00 (s, 3H, OCH3), 4.65 (broad s, 1H of CH3), 6.71 (s. 1H Ar), 7.22 (s, 1H Ar). - Palladium couplings, on the iminochloride of type 17. 5-(3-chlorophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17a
A mixture of 5-chloro-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one 16a (130 mg, 0.48 mmole), 3-chlorobenzene boronic acid (90.8 mg, 0.58 mmole),
K3PO, (118 mg, 0.56 mmole), tetrakis(triphenylphosphine) Pd (0) (15 mg, 0.01 mmole) in 3 ml of DMF was heated at 115°C for 12 h under an inert atmosphere, then cooled to room temperature. 30 ml of water were added and the mixture was extracted with 3 x 30 ml of Et;0. The organic fractions were dried Na>SOq, evaporated to dryness, purified by chromatography (EtOAc). Recrystallization was in EtOH. The reaction produced 103 mg of the above product in the form of white crystals. Yield : 62%. MP : 109-111°C. 'H NMR (CDCl;3, 200 MHz) : d 3.41 (s, 3H, NCH3), 3.77 (s, 3H, OCH3), 3.99 (s, 3H,
OCHj3), 4.34 (AB system, 7d = 1.02, Jag = 10, 2H, CHa3), 6.67 (s, 1H Ar), 6.79 (s, 1H
Ar), 7.19-7.69 (m, 4H Ar). 7,8-dimethoxy-1-methyl-5-(3-pyridyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17b
By replacing 3-chlorobenzene boronic acid in example 17a by pyridine-3-boronic-1,3- propanediol cyclic acid ester and proceeding in the same manner, the above product was obtained. Yield: 58%. MP : 177-179°C. '"H NMR (CDCl;, 200 MHz) : d 3.42 (s, 3H,
NCH3), 3.76 (s, 3H, OCH3), 3.99 (s, 3H, OCH3), 4.32 (AB system, 7d = 1.03, Jag = 10, 2H, CH3), 6.67 (s, 1H Ar), 6.80 (s, 1H Ar), 7.34-7.40 (m, 1H Ar), 8.03-8.09 (m, 1H
Ar), 8.68-8.71 (m, 2H Ar). 7,8-dimethoxy-1-methyl-5-(3-nitrophenyl)-1,3-dihydro-1,4-benzodiazepin-2-one,
By replacing 3-chlorobenzene boronic acid in example 17a by 3-nitrobenzene boronic acid and proceeding in the same manner, the above product was obtained. Yield : 43%.
MP : 152-155°C. 'H NMR (CDCl, 300 MHz) : d 3.43 (s, 3H, NCH3), 3.76 (s, 3H,
OCH3), 4.01 (s, 3H, OCH3;), 4.35 (AB system, 2d = 1.03, Jag = 10, 2H, CH}), 6.64 (s, 1H Ar), 6.82 (s, 1H Ar), 7.59-7.64 (m, 1H Ar), 8.08-8.12 (m, 1H Ar), 8.31-8.35 (m, 1H Ar), 8.49-8.51 (m, 1H Ar). 5-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- 2benzonitrile, 17d
By replacing 3-chlorobenzene boronic acid in example 17a by 3-cyano-4-[(4- methoxybenzyl)oxy]phenylboronic acid 37¢ and proceeding in the same manner, the above product was obtained. Yield : 20%. '"H NMR (DMSO, 300 MHz) : d 3.32 (s, 3H,
NCH;), 3.65 (s, 3H, OCH3), 3.70-3.77 (m, 4H, 1HCH, + BnOCHj3) 3.90 (s, 3H, OCH3;), 4.51 (m, 1HCH,), 5.26 (s, 2H, PhCH3), 6.70 (s, 1H Ar), 6.98 (m, 2HBn), 7.09 (s, 1H
Ar), 7.43 (m, 2HBn), 7.55-7.87 (m, 3H Ar). 5-(3-acetylphenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17e
By replacing 3-chlorobenzene boronic acid in example 17a by 3-acetylbenzene boronic acid and proceeding in the same manner, the above product was obtained. Yield : 43%.
MP : 148-150°C. '"H NMR (CDCl, 200 MHz) : d 2.64 (s, 3H, CH3CO), 3.42 (s, 3H,
NCH3), 3.74 (s, 3H, OCHj;), 4.00 (s, 3H, OCH3), 4.30 (AB system, 2d = 1.03, J, = 10, 2H, CHj), 6.65 (s, 1H Ar), 6.81 (s, 1H Ar), 7.48-8.23 (m, 4H Ar). 5-(4-isoquinolinyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17f
By replacing 3-chlorobenzene boronic acid in example 17a by 2-(isoquinolin-4-yl)- 4,4,5,5-tetramethyl-1,3-dioxaborolane and proceeding in the same manner, the above product was obtained. Yield : 34%. MP : 131-135°C. 'H NMR (CDCl, 300 MHz) : d 3.53 (s, 3H, NCH3), 3.55 (s, 3H, OCH3), 4.00 (s, 3H, OCH3), 4.50 (AB system, 7d =
1,00, Jag = 10.5, 2H, CH), 6.45 (s, 1H Ar), 6.84 (s, 1H Ar), 7.60-7.69 (m, 2H Ar), 7.89-8.08 (m, 2H Ar), 8.55 (s, 1H Ar), 9.32 (s, 1H Ar). : 7,8-dimethoxy-5-(3-hydroxymethylphenyl)-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 17g
By replacing 3-chlorobenzene boronic acid in example 17a by 3-hydroxymethylbenzene boronic acid and proceeding in the same manner, the above product was obtained. Yield : 25%. MP : 143-146°C. '"H NMR (DMSO, 300 MHz) : d 3.44 (s, 3H, NCH), 3.63 (s, 3H, OCHj;), 4.01 (s, 3H, OCH3), 4.35 (AB system, 7d = 0.25, Jag = 12.8, 2H, CH»), 4.60 (s, 2H, CH,0OH) 6.69 (s, 1H Ar), 6.91 (s, 1H Ar), 7.61-7.74 (m, 4H Ar). 7,8-dimethoxy-5-(3-hydroxymethylphenyl)-1-methyl-3-propyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 17h 21 mg (0.52 mmole) of 60% NaH in oil were added at 0°C and under an inert atmosphere to a solution of 140 mg (0.47 mmole) of 7,8-dimethoxy-5-(3- hydroxymethylphenyl)-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one 17g in 5 ml of
DMF. The mixture was stirred at room temperature for 1 h. At 0°C, 50 pl of bromopropane were added dropwise. The mixture was stirred overnight at room temperature. 50 ml of H,O were added and the mixture was extracted wtih 3 x 50 ml of
EtOAc and dried on MgSQO,4. The EtOAc was evaporated and the product was purified by chromatography on silica gel (EtOAc/Hexane 1:1 then EtOAc). Recrystallization was in CHCl; / cHex. Yield : 11%. MP : 134-136°C. '"H NMR (DMSO, 200 MHz) : d 0.85-0.93 (m, 3H, CH3), 1.35-1.55 (m, 2H, CH,CH3), 1.97-2.01 (m, 2H, CHCH3), 3.31 (s, 3H, NCH3), 3.42-3.47 (m, 1H, CHCH>), 3.62 (s, 3H, OCH3), 3.88 (s, 3H, OCH3;), 4.50 (d, J = 5.6, 2H, CH,OH), 5.21 (t, J = 5.6, 1H, OH), 6.66 (s, 1H Ar), 7.08 (s, 1H
Ar), 7.38-7.53 (m, 4H Ar). Mass : (M + H)" = 383.27. 5-(3-aminophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2- one, 17i
7,8-dimethoxy-1-methyl-5-(3-nitrophenyl)-1,3-dihydro-1,4-benzodiazepin-2-one 17¢ (100 mg, 0.28 mmole) , 10 mg of Pd/C (10 %), in 2 ml of methanol were stirred under hydrogen pressure for 8 h, then filtered on cellite, washed with 3 x 25 ml of methanol, evaporated to dryness and purified by chromatography (EtOAc) to give the above product in the form of white crystals. Yield : 77%. 'H NMR (CDCls, 300 MHz) : d 1.60 (s, 2H exchangeable NHy), 3.40 (s, 3H, NCH3), 3.78 (s, 3H, OCHj3), 3.99 (s, 3H, OCHa), 4.38 (AB system, 7d = 1.03, Jog = 10, 2H, CH), 6.76 (m, 3H Ar), 6.89-6.91 (d, 1H
Ar), 7.10 (s, 1H Ar), 7.14-7.22 (t, 1H Ar). 5-(3,4-dichlorophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2- one, 17
By replacing 3-chlorcbenzene boronic acid in example 17a by 3,4-dichlorophenyl boronic acid and proceeding in the same manner, the above product was obtained.
Yield : 25%. MP : 174-177°C. '"H NMR (CDCls, 200 MHz) : d 3.44 (s, 3H, NCH3), 3.81 (s, 3H, OCH3), 4.02 (s, 3H, OCHs3), 4.50 (AB system, ?d = 1.00, Jog = 10.5, 2H, CH)), 6.67 (s, 1H Ar), 6.82 (s, 1H Ar), 7.52 (s, 2H Ar), 7.83 (s, 1H Ar). 7,8-dimethoxy-1-methyl-5-(3-methylphenyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17k
By replacing 3-chlorobenzene boronic acid in example 17a by 3-methylbenzene boronic acid and proceeding in the same manner, the above product was obtained. Yield : 20%.
MP: 119-121°C. '"H NMR (CDCl3, 300 MHz): d 2.39 (s, 3H, CH3), 3.40 (s, 3H,
NCHs3), 3.75 (s, 3H, OCHs), 3.98 (s, 3H, OCH3), 4.31 (AB system, 7d = 0.99, Jzg = 10.3, 2H, CH,), 6.71 (s, 1H Ar), 6.78 (s, 1H Ar), 7.27-7.28 (m, 2H Ar), 7.35-7.38 (m, 1H Ar), 7.53 (s, 1H Ar). 5-(3-formylphenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 171
By replacing 3-chlorobenzene boronic acid in example 17a by 3-formylbenzene boronic acid and proceeding in the same manner, the above product was obtained. Yield: 25%,
MP : 175-178°C. 'H NMR (CDCl;, 200 MHz) : d 3.45 (s, 3H, NCH3), 3.77 (s, 3H,
OCH3), 4.03 (s, 3H, OCHj3), 4.39 (AB system, 2d = 1.01, Jag = 10.5, 2H, CH,), 6.68 (5, 1H Ar), 6.84 (s, 1H Ar), 7.60-7.67 (m, 1H Ar), 8.01-8.05 (m, 2H Ar), 8.18 (s, IH Ar), 10.09 (s, IH, CHO). : 5-[3-(benzylaminomethyl)phenyl]-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one hydrochloride, 17m
A mixture of 200 mg (0.53 mmole) of 5-(3-formylphenyl)-7,8-dimethoxy-1-methyl-1,3- dihydro-1,4-benzodiazepin-2-one 171ly, 60 pl (0.56 mmole) of benzylamine in 5 ml of
MeOH was heated overnight under reflux. At 0°C, 53 mg (1.4 mmoles) of NaBH, was added in small portions. The reaction was stirred at room temperature for 1 h. The methanol was evaporated. 30 ml of H,O were added and the mixture was extracted with 3 x 50 ml of EtOAc. 10 ml of CH,Cl, were added and HCI was bubbled in at 0°C until the solution became saturated. The solvent was evaporated and the residue was triturated in Et,0, discarding the supernatant several times. Recrystallization was in a mixture of
EtOH/Et,O. The crystals were filtered and dried. Yield : 75%. MP : 88-90°C. '"H NMR (DMSO, 200 MHz) : d 3.41 (s, 3H, NCHj3), 3.50-3.69 (m, 4H, 1HCH; + OCHj3), 3.98 (s, 3H, OCHa), 4.13-4.23 (m, 4H, 2CH,Ph), 4.47-4.54 (m, 1H, 1HCH3), 6.70 (s, 1H Ar), 7.23 (s, 1H Ar), 7.38-7.98 (m, 9H Ar). Mass : (M+H)" "= 430.18.
N-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) phenyljacetamide, 17n
By replacing 3-chlorobenzene boronic acid in example 17a by 3-acetamidobenzene boronic acid and proceeding in the same manner, the above product was obtained.
Yield : 15%. 'H NMR (DMSO, 300 MHz) : d 2.01 (s, 3H, CH3CO), 3.31 (s, 3H, NCH3), 3.66 (s, 3H, OCH3), 3.90 (s, 3H, OCH};), 4.12 (AB system, 7d = 0.78, Jap = 10.17, 2H,
CH,), 6.71 (s, 1H Ar), 7.08 (s, 1H Ar), 7.29-7.78 (m, 4H Ar), 10.01 (s, 1H, NHAC). 7,8-dimethoxy-1-methyl-5-(3,5-methylenedioxyphenyl)-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 170
By replacing 3-chlorobenzene boronic acid in example 17a by 3,5- methylenedioxybenzene boronic acid and proceeding in the same manner, the above product was obtained. Yield : 55%. MP : 161-162°C. 'H NMR (CDCl, 300 MHz) : d 3.39 (s, 3H, NCH3), 3.74-3.79 (m, 4H, HCH; + OCH3), 3.98 (s, 3H, OCH3), 4.72-4.76 (m, 1HCH,), 6.03 (s, 2H, CH;0,), 6.75-7.28 (m, SH Ar). - Regioselective halogenation and synthesis of benzodiazepines of type 24
Tert-butyl(2-methoxy-5-nitrophenoxy)diphenylsilane, 18a
At 0°C under an inert atmosphere, a solution of 7 g (41 mmoles) of 3-hydroxy-4- methoxynitrobenzene dissolved in 50 ml of DMF was added to a solution of 2 g (50 mmoles) of sodium hydride dissolved in 50 ml of DMF. After 30 minutes at room temperature, 12.9 ml (50 mmoles) of fert-butylchlorodiphenyl silane were added dropwise at 0°C. The reaction was stirred at room temperature for 12 hours, then diluted with 10 volumes of water and extracted with 3 x 50 ml of Et,O. The organic phase was washed with 100 ml of 1 M HCI and NaCl (sat), dried on Na;SOs, filtered and evaporated to dryness to give the above product. Yield : 82%. 'H NMR (CDCls, 200
MHz) : d 1.18 (s, 9H, C(CHs)3), 3.61 (s, 3H, OCH3), 6.74-6.78 (d, 1H Ar), 7.34-7.82 (m, 12H Ar).
Tert-butyl(2-methoxy-4-nitrophenoxy)diphenylsilane, 18b
At 0°C under an inert atmosphere, a solution of 1 g (6.45 mmoles) of 4-nitrocatechol dissolved in 5 ml of DMF was added to a solution of 480 mg (7.10 mmoles) of imidazole dissolved in 5 ml of DMF. After 30 minutes at room temperature, 1.77 ml (6.77 mmoles) of tert-butylchlorodiphenyl silane were added dropwise at 0°C. The reaction was stirred at room temperature for 12 hours, then diluted with 10 volumes of water and extracted with 3 x 50 ml of Et,O. The organic phase was washed with 100 ml of 1 M HCI and NaCl (sat), dried on Na,SOs, filtered, and evaporated to dryness to yield a brown oil. Said oil was dissolved in 30 ml of DMF, then 2.86 g (20.69 mmoles) of
K,CO3; were added and the mixture was stirred for 30 minutes. lodomethane (1.37 ml,
22.04 mmoles) was added and the reaction left for 2 hours, then diluted with 10 volumes of water and extracted with 3 x 100 ml of Et,0. The organic phase was washed with 100 ml of 10 % NaOH, dried on Na;SQ,, filtered, evaporated to dryness and purified by chromatography on silica gel (hexane/CH,Cly, 2:1). Yield : 57%. 'H NMR (CDCl;, 200
MHz): d 1.15 (s, 9H, C(CH3)3), 3.58 (s, 3H, OCHj3), 6.71-6.76 (d, 1H Ar), 7.36-7.82 (m, 12H Ar). 3-{[tert-butyl(diphenyl)silyl]oxy}-4-methoxyaniline, 19a
By replacing 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one 6b in example 6e by fert-butyl(2-methoxy-5- nitrophenoxy)diphenylsilane 18a and proceeding in the same manner, the above product was obtained. Yield : 98%. '"H NMR (CDCl;, 300 MHz) : d 1.12 (s, 9H, C(CH3)3), 3.59 (s, 3H, OCH3), 6.23 (s, 1H Ar), 6.24-6.30 (d, 1H Ar), 6.62-6.65 (d, 1H Ar), 7.28-7.75 1S (m, 10H Ar). 4-{[tert-butyl(diphenyl)silyl]oxy}-3-methoxyaniline, 19b
By replacing 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one 6b in example 6e by tert-butyl(2-methoxy-4- nitrophenoxy)diphenylsilane 18b and proceeding in the same manner, the above product was obtained. Yield : 95%. '"H NMR (CDCls, 200 MHz) : d 1.08 (s, 9H, C(CH3)3), 3.53 (s, 3H, OCH3), 5.98-6.02 (m, 1H Ar), 6.18 (s, 1H Ar), 6.50-6.54 (m, 1H Ar), 7.37-7.72 (m, 10H Ar). 3-(2-amino-5-{[tert-butyl(diphenyl)silyl]oxy}-4-methoxybenzoyl)benzonitrile, 20b
At 0°C under an inert atmosphere, 3.5 g (9.22 mmoles) of 4-{|tert- butyl(diphenyl)silylJoxy}-3-methoxyaniline 19b dissolved in 10 ml of 12- dichloroethane, 2.35 g (18.36 mmoles) of isophthalonitrile, and 1.34 g (10.00 mmoles) of AICI; were added to a solution of 10 ml of boron tribromide (1IM/ CH,Cl,, 10 mmoles) and stirred at room temperature for 30 minutes. The dichloromethane was evaporated. The mixture was heated under reflux for 12 hours, then cooled. 10 ml of 1
M HCI were added at 0°C and the mixture was stirred at 75°C for 1 hour. After adding 50 ml of water, the mixture was extracted with 3 x 100 ml of CH,Cl,. The organic fractions were dried on Na,SO,, filtered, evaporated to dryness and purified by chromatography on silica gel (EtOAc/hexane, 1:3). Yield : 30%. '"H NMR (CDCl;, 300
MHz) : d 1.06 (s, 9H, C(CH3)3), 3.77 (s, 3H, OCH3), 6.09 (s, 1H Ar), 6.17 (broad s, 2H,
NH), 6.47 (s, 1H Ar), 7.37-7.86 (m, 14H Ar). 3-(2-amino-4-{ [tert-butyl(diphenyl)silylJoxy}-5-methoxybenzoyl)benzonitrile, 20a
By replacing 4-{[tert-butyl(diphenyl)silyljoxy}-3-methoxyaniline 19b in example 20b by 3-{[tert-butyl(diphenyl)silylJoxy}-4-methoxyaniline 19a and proceeding in the same manner, the above product was obtained. Yield : 38%. '"H NMR (CDCl; 300 MHz) : d 1.11 (s, 9H, C(CHa)3), 3.41 (s, 3H, OCH3), 5.90 (broad s, 2H, NH3), 6.01 (s, 1H Ar), 6.67 (s, 1H Ar), 7.39-7.96 (m, 14H Ar). 2-bromo-N-[5-{[tert-butyl(diphenyl)silyl]oxy}-2-(3-cyanobenzoyl)-4- methoxyphenyl]acetamide, 21b
To a solution of 3-(2-amino-5-{[tert-butyl(diphenyl)silylJoxy}-4- methoxybenzoyl)benzonitrile 20b (0.4 g, 0.79 mmole) in 5 ml of dichloromethane at 0- 5°C, bromoacetate bromide (82 ??, 0.94 mmole) was added and then 10% Na,CO; (2.4 ml) was added dropwise. The reaction was stirred at this temperature for 1 hour. The two phases were separated and the organic phase was washed with 10 ml of water, dried on NapSOy, filtered and evaporated to dryness (455 mg). Yield : 92%. 'H NMR (CDCl, 200 MHz) : d 1.15 (s, 9H, C(CH3)3), 3.29 (s, 3H, OCH}), 3.95 (s, 2H; CH,), 6.75 (s, 1H
Ar), 7.37-7.74 (m, 14H Ar), 8.23 (s, 1H Ar), 11.55 (broad s, 1H, NH). 2-bromo-N-[4-{[tert-butyl(diphenyl)silyl]oxy}-2-(3-cyanobenzoyl)-5- methoxyphenyl]acetamide, 21a
By replacing 3-(2-amino-5-{[tert-butyl(diphenyl)silylJoxy}-4- methoxybenzoyl)benzonitrile 20b in example 21b by 3-(2-amino-4-{[ters-
butyl(diphenyl)silylJoxy}-5-methoxybenzoyl)benzonitrile 20a and proceeding in the same manner, the above product was obtained. Yield : 90%. 'H NMR (CDCl, 300
MHz) : d 1.14 (s, 9H, C(CHs)s), 3.28 (s, 3H, OCHj;), 3.94 (s, 2H; CH), 6.74 (s, 1H Ar), 7.37-7.87 (m, 14H Ar), 8.23 (s, 1H Ar), 11.52 (broad s, 1H, NH). 3-(7-hydroxy-8-methoxy-2-o0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 22b 2-bromo-N-[5-{[fert-butyl(diphenyl)silylJoxy}-2-(3-cyanobenzoyl)-4- methoxyphenyl]acetamide 21b (0.5 g, 0.79 mmole) in solution in NH3 (7N)/MeOH (10 ml) was stirred in a CaCl, tube for 30 minutes at 0°C then for 30 minutes at room temperature. It was then heated under reflux for 2 hours, evaporated to dryness and purified by chromatography on silica gel (MeOH/CH,Cl,, 1:9). Yield : 95%. 'H NMR (DMSO-ds, 300 MHz) : d 3.63 (s, 3H, OCH3), 4.12 (broad s, 2H; CH3), 6.65 (s, 1H Ar), 6.72 (s, 1H Ar), 7.65-7.99 (m, 4H Ar), 10.14 (broad s, 1H, NH), 10.33 (broad s, 1H,
OH). 3-(8-hydroxy-7-methoxy-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 22a
By replacing 2-bromo-N-[5-{[fert-butyl(diphenyl)silylJoxy}-2-(3-cyanobenzoyl)-4- methoxyphenyl]acetamide 21b in example 22b by 2-bromo-N-[4-{[tert- butyl(diphenyl)silylJoxy}-2-(3-cyanobenzoyl)-5-methoxyphenyl]acetamide 21a and proceeding in the same manner, the above product was obtained. Yield : 30%. 'H NMR (DMSO-dg, 200 MHz) : d 3.64 (s, 3H, OCH3), 4.13 (broad s, 2H; CHa), 6.66 (s, 1H Ar), 6.72 (s, 1H Ar), 7.61-2.25 (m, 4H Ar), 10.13 (broad s, 1H, NH), 10.33 (broad s, 1H,
OH). 3-(6-bromo-7-hydroxy-8-methoxy-2-o0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5- ylbenzonitrile, 23b
A mixture of 3-(7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 22b (150 mg, 0.49 mmole) and N-bromosuccinimide (90 mg, 0.51 mmole) in acetic acid was heated at 60°C for 2 hours, evaporated to dryness and crystallized in Et,0. Yield : 70%. '"H NMR (DMSO-dg, 300 MHz) : d 3.92 (s, 3H,
OCHj;), 4.20 (AB system, 2d = 0.6, Jag = 10 Hz, 2H, NCH,), 6.91 (s, 1H Ar), 7.61-7.93 (m, 4H Ar + OH), 10.36 (broad s, 1H, NH). 3-(9-iodo-8-hydroxy-7-methoxy-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 23a
By replacing 3-(7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 22b in example 23b by 3-(8-hydroxy-7-methoxy-2-o0xo-2,3-dihydro-1H- 1,4-benzodiazepin-5-yl)benzonitrile 22a and N-bromosuccinimide by N- iodosuccinimide and proceeding in the same manner, the above product was obtained.
Yield : 83%. 'H NMR (DMSO-ds, 200 MHz) : d 3.72 (s, 3H, OCH3), 4.08 (m 2H,
NCH3), 6.78 (s, 1H Ar), 7.61-7.93 (m, 4H Ar ), 9.22 (broad s, 1H, OH), 10.61 (broad s, 1H, NH). 3-(6-iodo-7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 23c
By replacing N-bromosuccinimide in example 23b by N-iodosuccinimide and proceeding in the same manner, the above product was obtained. Yield : 65%. 'H NMR (DMSO-dg, 300 MHz): d 3.72 (s, 3H, OCHj3), 4.11 (m, 2H, NCH,), 6.77 (s, 1H Ar), 7.65-8.03(m, 4H Ar ), 9.27 (broad s, 1H, OH), 11.13 (broad s, 1H, NH). 3-(9-bromo-8-hydroxy-7-methoxy-2-oxo-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 23d
By replacing 3-(7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile 22b in example 23b by 3-(8-hydroxy-7-methoxy-2-o0xo-2,3-dihydro-1H- 1,4-benzodiazepin-5-yl)benzonitrile 22a and proceeding in the same manner, the above product was obtained. Yield : 86%. 'H NMR (DMSO-d¢, 200 MHz) : d 3.92 (s, 3H,
OCH3), 4.18 (m 2H, NCH3), 6.84 (s, 1H Ar), 7.61-7.93 (m, 4H Ar ), 9.22 (broad s, 1H,
OH), 11.05 (broad s, 1H, NH).
3-(6-bromo-7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 24b 3-(6-bromo-7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yDbenzonitrile 23b (120 mg, 0.31 mmole) was dissolved in 2 ml of DMF, then 131 mg (0.95 mmole) of K,CO; were added and the reaction was stirred for 30 minutes.
Iodomethane (44 OL, 0.71 mmole) was added and allowed to react for 6 hours. A water/ice mixture was added and the precipitate was filtered. Yield : 70%. 'H NMR (CDCl; 300 MHz) : d 3.39 (s, 3H, NCH3), 3.88 (s, 3H, OCH3), 4.00 (s, 3H, OCH3), 4.30 (AB system, 7d = 0.9, Jag = 10 Hz, 2H, NCH,), 6.84 (s, 1H Ar), 7.49-7.51 (t, 1H
Ar), 7.67-7.70 (d, 1H Ar), 7.74 (s, 1H Ar), 7.81-7.83 (d, 1H Ar). 3-(9-iodo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- ylbenzonitrile, 24a
By replacing 3-(6-bromo-7-hydroxy-8-methoxy-2-oxo0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzonitrile 23b in example 24b by 3-(9-iodoo-8-hydroxy-7- methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 23a and proceeding in the same manner, the above product was obtained. Yield: 91%. 'H NMR (CDCl, 200 MHz) : d 3.31 (s, 3H, NCH3), 3.77 (s, 3H, OCH3), 3.97 (s, 3H, OCH3), 4.31 (AB system, ?d = 1.0, Jag = 10 Hz, 2H, NCH), 6.67 (s, 1H Ar), 7.53-8.03 (m, 4H Ar). 3-(6-iodo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 24¢
By replacing 3-(6-bromo-7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1 4- benzodiazepin-5-yl)benzonitrile 23b in example 24b by 3-(6-iodoo-7-hydroxy-8- methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 23¢ and proceeding in the same manner, the above product was obtained. Yield : 58%. 'H NMR (CDCl, 200 MHz) : d 3.31 (s, 3H, NCHy), 3.78 (s, 3H, OCHj3), 3.96 (s, 3H, OCHa), 4.28 (AB system, 7d = 1.0, Jap = 10 Hz, 2H, NCH), 6.67 (s, 1H Ar), 7.53-8.03 (m, 4H Ar).
3-(9-bromo-7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5- yD)benzonitrile, 24d
By replacing 3-(6-bromo-7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzonitrile 23b in example 24b by 3-(9-bromo-7-hydroxy-8- methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-S-yl)benzonitrile 23d and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCl;, 300 MHz) : d 3.31 (s, 3H, NCH3), 3.79 (s, 3H, OCH3), 3.99 (s, 3H, OCHj3),4.30 (AB system, ?d = 1.0, Jag = 10 Hz, 2H, NCH), 6.65 (s, 1H Ar), 7.57-8.02 (m, 4H Ar). - Palladium couplings, on benzodiazepinones of type 25. 3-(7,8-dimethoxy-1-methyl-2-oxo0-6-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 25b
A mixture of 67 mg (0.162 mmole) of 3-(6-bromo-7,8-dimethoxy-1-methyl-2-ox0-2,3- dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 24b, 39 mg (0.32 mmole) of benzene boronic acid, 63 mg (0.30 mmole) of K;POs, 19 mg (0.016 mmole) of tetrakis(triphenylphosphine) Pd(0) in 1 ml of DMF was heated at 115°C for 16 hours, then cooled to room temperature, diluted with 10 volumes of water and extracted with 3 x 100 ml of Et,O. The organic phase was dried on Na;SO,, filtered, evaporated to dryness and purified by chromatography on silica gel (CH,Cl/Et;0, 1:1). Yield : 40%. 'H NMR (CDCl, 200 MHz) : d 3.45 (s, 3H, NCH3), 3.50 (s, 3H, OCH3), 4.03 (s, 3H,
OCH3),4.50 (AB system, 7d = 0.8, Jag = 10 Hz, 2H, NCH), 6.87-7.51 (m, 7H Ar), 7.33-7.80 (m, 3H Ar). 3-(7,8-dimethoxy-1-methyl-2-0x0-9-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 25a
By replacing 3-(6-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1 4- benzodiazepin-5-yl)benzonitrile 24b in example 25b by 3-(9-iodo-7,8-dimethoxy-1- methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 24d and proceeding in the same manner, the above product was obtained. Yield : 51%. 'H NMR (CDCl,
300 MHz) : d 2.44 (s, 3H, NCH), 3.63 (s, 3H, OCHj3;), 3.80 (s, 3H, OCH), 4.45 (AB system, 7d = 1.0, Jap = 10 Hz, 2H, NCH), 6.69 (s, 1H Ar), 7.41-8.27 (m, 9H Ar).
Tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-o0xo0-2,3-dihydro-1H-1,4- benzodiazepin-9-yl)phenyl]prop-2-ynylcarbamate, 25¢
By replacing 5-(3-bromophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one 4b in example 6a by 3-(9-iodo-7,8-dimethoxy-1-methyl-2-0x0-2,3- dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 24a and proceeding in the same manner, the above product was obtained. Yield : 55%. 'H NMR (CDCl;, 200 MHz) : d 1.48 (s, 9H, C(CH3)s3), 3.36 (s, 3H, NCH3), 3.75 (s, 3H, OCH), 4.03 (s, 3H, OCH3), 4.23-4.26 (d, 2H, NHCH3), 4.32 (AB system, ?d = 1.0, Jag = 10 Hz, 2H, CH,), 6.61 (s, 1H Ar), 7.51-7.99 (m, 4H Ar).
Methyl (2E)-3-[5-(cyanophenyl)-7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4- benzodiazepin-9-yl)phenyljacrylate, 25d
In a tube sealed under argon, 92 mg (0.2 mmole) of 3-(9-iodo-7,8-dimethoxy-1-methyl- 2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 24a was dissolved in 0.5 ml of DMF. One mg (4.4 umoles) of palladium acetate, 23 01 (0.25 mmole) of methyl acrylate and 53 pl (0.22 mmole) of tributylamine were added. The solution was placed in a microwave at 200 watts for 10 minutes. Water was added, the mixture was extracted with EtOAc, dried on Na;SO,, evaporated to dryness, and purified by chromatography on silica gel (CH2Clo/EtOAc, 2:8). Yield : 27%. '"H NMR (CDCls, 200
MHz): d 3.13 (s, 3H, NCH3), 3.78 (s, 3H, OCHj;), 3.84 (s, 3H, OCH3), 3.93 (s, 3H,
OCH3), 4.37 (AB system, 2d = 1.0, Jog = 10 Hz, 2H, CH,), 6.65-6.73 (m, 2H Ar), 7.51- 7.99 (m, SH Ar).
Tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-14- benzodiazepin-6-yl)phenyl]prop-2-ynylcarbamate, 25¢
By replacing 5-(3-bromophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1 4- benzodiazepin-2-one 4b in example 6a by 3-(6-iodo-7,8-dimethoxy-1-methyl-2-0x0-2 3-
dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile 24c¢ and proceeding in the same manner, the above product was obtained. Yield : 59%. 'H NMR (CDCl, 200 MHz) : d 1.48 (s, 9H, C(CH3)s3), 3.36 (s, 3H, NCH), 3.75 (s, 3H, OCHj;), 4.02 (s, 3H, OCH3), 4.23-4.26 (d, 2H, NHCH,), 4.30 (AB system, ?d = 1.0, Jap = 10 Hz, 2H, CH,), 6.61 (s, 1H Ar), 7.55-8.01 (m, 4H Ar). [9-(3-aminoethynyl)-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-14- benzodiazepin-5-yl|benzonitrile, 25f
A mixture of 25 mg (0.05 mmole) of tert-butyl 3-[5-(cyanophenyl)-(7,8-dimethoxy-1- methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-9-yl)phenyl]prop-2-ynylcarbamate 25c¢, (40 OL, 0.52 mmole) of trifluoroacetic acid and 2 ml of CH,Cl, was stirrred under an inert atmosphere at room temperature for 2 hours, then evaporated tc dryness. The product was crystallized in EtOAc/hexane. Yield : 97%. '"H NMR (DMSO-de, 200
MHz): d 3.29 (s, 3H, NCH3), 3.74 (s, 3H, OCH3), 3.95 (s, 3H, OCH3), 4.17 (m, 2H,
CH;), 4.26 (AB system, 7d = 0.7, Jag = 11 Hz, 2H, NCH3), 6.90 (s, 1H Ar), 7.66-8.07 (m, 4H Ar), 8.41 (broad s, 3H, NH>). [6-(3-aminoethynyl)-7,8-dimethoxy-1-methyl-2-0xo0-2,3-dihydro-1H-14- benzodiazepin-5-yl]benzonitrile, 25g
By replacing tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-0x0-2,3- dihydro-1H-1,4-benzodiazepin-9-yl)phenyl]prop-2-ynylcarbamate 25¢ in example 25f by tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-6-yl)phenyl]prop-2-ynylcarbamate 25e and proceeding in the same manner, the above product was obtained. Yield : 96%. '"H NMR (DMSO-d, 200 MHz) : d 3.33 (s, 3H, NCH3), 3.75 (s, 3H, OCHj3), 4.01(s, 3H, OCH3), 4.20 (m, 2H, CH,), 4.25 (AB system, 7d = 0.7, Jag = 11 Hz, 2H, NCH), 6.63 (s, 1H Ar), 7.53-7.99 (m, 4H Ar), 8.52 (broad s, 3H, NH).
Synthesis of benzodiazepinones of type 29 4-bromo-3,5-dimethoxyaniline, 26a
3.06 g (20 mmoles) of 3,5-dimethoxyaniline were dissolved in 50 ml of CH,Cl,. The mixture was cooled to -10°C and 8.19 g (20 mmoles) of 2,4,4,6-tetrabromocyclohexa- 2,5-dienone were added one spatula at a time, without allowing the temperature to rise above —5°C. The reaction was then returned to room temperature and stirred for 3 hours, evaporated to dryness, triturated in ether, filtered and washed with ether. The reaction produced 3.20 g (13.8 mmoles) of the above product. Yield : 69%. 'H NMR (CDCls, 300 MHz) : d 3.74 (s, 2H, NH;), 3.84 (s, 6H, 2 x OCH3), 5.96 (s, 2H Ar). 3-(2-amino-4-methoxybenzoyl)benzonitrile, 27a
By replacing 3,4-dimethoxyaniline in example 2a by 3-methoxyaniline and proceeding in the same manner, the above product was obtained. Yield : 42%. '"H NMR (CDCl3, 300 MHz) : d 3.82 (s, 3H, OCHj3), 6.17 (s, 1H Ar), 6.46 (s, 2H exchangeable NH,), 6.21-6.24 (d, 1H Ar), 7.54-7.59 (m, 1H Ar), 7.75-7.95 (m, SH Ar). 3-(2-amino-5-methoxybenzoyl)benzonitrile, 27b
By replacing 3,4-dimethoxyaniline in example 2a by 4-methoxyaniline and proceeding in the same manner, the above product was obtained. Yield : 48%. 'H NMR (CDCl, 200 MHz) : d 3.70 (s, 3H, OCHj3), 5.88 (s, 2H exchangeable NH;), 6.75-6.84 (m, 2H
Ar), 7.05-7.11 (m, 1H Ar), 7.59-7.73 (m, 2H Ar), 7.83-7.99 (m, 2H Ar). 3-(2-amino-6-methoxybenzoyl)benzonitrile, 27¢
This product was obtained at the same time as 3-(2-amino-4- methoxybenzoyl)benzonitrile 27a. Yield : 22%. 'H NMR (CDCls, 300 MHz) : d 3.49 (s, 3H, OCHj;), 5.05 (s, 2H exchangeable NH3), 6.23-6.25 (d, 1H Ar), 6.38-6.42 (d, 1H
Ar), 7.20-7.28 (m, 1H Ar), 7.50-7.55 (m, 2H Ar), 7.74-7.77 (m, 1H Ar), 7.90-7.96 (m, 1H Ar), (2-amino-4-methoxyphenyl)(phenyl)methanone, 27d
By replacing isophthalonitrile in example 2a by benzonitrile, and 3,4-dimethoxyaniline by 3-methoxyaniline, and proceeding in the same manner, the above product was : obtained. Yield : 68%. '"H NMR (CDCls, 300 MHz) : d 3.82 (s, 3H, OCH3), 6.15-6.19 (m, 2H Ar), 6.36 (s, 2H exchangeable NH;), 7.38-7.50 (m, 4H Ar), 7.57-7.62 (m, 2H
Ar). (2-amino-6-methoxyphenyl)(phenyl)methanone, 27¢
This product was obtained at the same time as (2-amino-4- methoxyphenyl)(phenyl)methanone 27d. Yield : 16%. 'H NMR (CDCls;, 300 MHz) : d 3.53 (s, 3H, OCH3), 4.61 (s, 2H exchangeable NH,), 6.27-6.30 (d, 1H Ar), 6.37-6.40 (d, 1H Ar), 7.16-7.22 (t, 1H Ar), 7.38-7.43 (m, 2H Ar), 7.49-7.53 (m, 1H Ar), 7.74-7.77 (m, 2H Ar). (2-amino-3-bromo-4.5-dimethoxyphenyl)(phenyl)methanone, 27f 15 g of 40% HBr by weight in water were added dropwise at 0°C to a solution of 900 mg (3.5 mmoles) of 2-amino-4,5-dimethoxybenzophenone 2¢ in 60 ml of DMSO. The reaction was heated at 60°C for 24 hours. 400 ml of water were added and the mixture was extracted with 4 x 200 ml of EtOAc and dried on MgSO4. The EtOAc was evaporated and the product was purified by chromatography on silica gel (EtOAc/hexane, 1:4). Yield : 65%. '"H NMR (CDCl, 300 MHz) : d 3.67 (s, 3H, OCH3), 3.97 (s, 3H, OCHj3), 6.59 (s, 2H, NH3), 7.06 (s, 1H Ar), 7.47-7.65 (m, SH Ar). Mass : (M + H)" = 335.98 + 337.98. 3-(2-amino-4,6-dimethoxybenzoyl)benzonitrile, 27g
By replacing 3,4-dimethoxyaniline in example 2a by 3,5-dimethoxyaniline and proceeding in the same manner, the above product was obtained. Yield : 55%. '"H NMR (CDCl; 300 MHz) : d 3.39 (s, 3H, OCH3), 3.78 (s, 3H, OCH3), 5.87(s, 1H Ar), 6.08 (s, 1H Ar), 6.36 (s, 2H exchangeable NH3), 7.61-7.97 (m, 4H Ar). 3-(6-amino-3-bromo-2,4-dimethoxybenzoyl)benzonitrile, 27h
By replacing 3,4-dimethoxyaniline in example 2a by 4-bromo-3,5-dimethoxyaniline 26a : and proceeding in the same manner, the above product was obtained. Yield : 59%. 'H
NMR (CDCl, 200 MHz) : d 3.49 (s, 3H, OCH3), 3.97 (s, 3H, OCHs), 5.89 (s, 1H Ar), 6.09 (s, 2H exchangeable NH,), 7.51-7.96 (m, 4H Ar). 3-(8-methoxy-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5S-yl)benzonitrile, 28a
A mixture of 3-(2-amino-4-methoxybenzoyl)benzonitrile 27a (4 g, 15.9 mmoles), ethyl glycinate. HCI (4 g, 28.6 mmoles), and 40 ml of anhydrous pyridine was heated under reflux in an inert atmosphere for 36 hours. Two 2 g fractions (14.3 mmoles) of ethyl glycinate. HCI, were added every 10 hours. After returning to room temperature, the mixture was evaporated to dryness. 200 m! of water were added and the mixture was extracted with 3 x 200 ml of dichloromethane. The organic phases were dried on Na,SO,, purified by chromatography (EtOAc) and recrystallinzed in EtOH/EtO, to give the above product in the form of colorless crystals. Yield : 18%. 'H NMR (CDCl, 300
MHz): d 3.90 (s, 3H, OCH3;), 4.35 (s 2H, CH3), 6.65 (m, 1H Ar), 6.73-6.77 (m, 1H Ar), 7.15-7.18 (d, 1H Ar), 7.48-7.54 (t, 1H Ar), 7.72-7.75 (m, 1H Ar), 7.80-7.84 (m, 1H
Ar), 7.86 (m, 1H Ar), 9.08 (s, 1H exchangeable, -NH). 3-(6-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28b
By replacing 3-(2-amino-4-methoxybenzoyl)benzonitrile 27a in example 28a by 3-(2- amino-6-methoxybenzoyl)benzonitrile 27¢ and proceeding in the same manner, the above product was obtained. Yield : 12%. 'H NMR (CDCls, 300 MHz) : d 3.53 (s, 3H,
OCHs), 4.37 (AB system, 7d = 0.87, Jag = 11 Hz, 2H, CH3), 6.73-6.80 (t, 2H Ar), 7.42- 7.53 (m, 2H Ar), 7.64-7.68 (m, 1H Ar), 7.72-7.75 (m, 2H Ar), 842 (s, 1H exchangeable, -NH). 3-(7-methoxy-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-S-yl)benzonitrile, 28¢
By replacing 3-(2-amino-4-methoxybenzoyl)benzonitrile 27a in example 28a by 3-(2- amino-5-methoxybenzoyl)benzonitrile 27b and proceeding in the same manner, the above product was obtained. Yield : 35%. 'H NMR (CDCl3, 300 MHz) : d 3.76 (s, 3H,
OCH), 4.35 (s, 2H, CH), 6.70 (s, 1H Ar), 7.12-7.13 (m, 1H Ar), 7.50-7.55 (t, 1H Ar), 7.73-7.76 (m, 1H Ar), 7.84-7.87 (m, 1H Ar), 7.92 (s, 1H Ar), 8.51 (s, 1H exchangeable, -NH). 6-methoxy-S-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28d
By replacing 3-(2-amino-4-methoxybenzoyl)benzonitrile 27a in example 28a by (2- amino-6-methoxyphenyl)(phenyl)methanone 27e and proceeding in the same manner, the above product was obtained. Yield : 68%. 'H NMR (CDCl;, 200 MHz) : d 3.48 (s, 3H, OCH3), 4.34 (AB system, ?d = 0.85, Jog = 11 Hz, 2H, CH3), 6.69-6.80 (m, 2H Ar), 7.26-7.48 (m, 6H Ar), 8.76 (s, 1H exchangeable, -NH). 7-methoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28e
By replacing 3-(2-amino-4-methoxybenzoyl)benzonitrile 27a in example 28a by (2- amino-4-methoxyphenyl)(phenyl)methanone 27d and proceeding in the same manner, the above product was obtained. Yield : 32%. 'H NMR (CDCls, 200 MHz) : d 3.72 (s, 3H, OCHs), 4.32 (s, 2H, CH3), 6.78 (s, 1H Ar), 7.08 (m, 2H Ar), 7.33-7.48 (m, 3H Ar), 7.55-7.60 (m, 2H Ar), 8.86 (s, 1H exchangeable, -NH). 9-bromo-7,8-dimethoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28f
By replacing 3-(2-amino-4-methoxybenzoyl)benzonitrile 27a in example 28a by (2- amino-3-bromo-4,5-dimethoxyphenyl)(phenyl)methanone 27f, and ethyl glycinate by methyl glycinate, and proceeding in the same manner, the above product was obtained.
Yield: 40%. '"H NMR (CDCl;, 300 MHz) : d 3.74 (s, 3H, OCH), 3.98 (s, 3H, OCH), 4.32 (m, 2H, CH,), 6.78 (s, 1H Ar), 7.41-7.67 (m, 6H, INH + 5H Ar). Mass : (M+H)" = 375.05 + 377.03. 3-(6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28g
By replacing 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile 2a in example 3a by 3-(2- amino-4,6-dimethoxybenzoyl)benzonitrile 27g and proceeding in the same manner, the above product was obtained. Yield : 42%. 'H NMR (DMSO, 300 MHz) : d 3.46 (s, 3H,
OCH3), 3.86 (s, 3H, OCH;), 4.18 (AB system, ?d = 0.6, Jag = 10 Hz, 2H, NCH), 6.45 (s, 1H, 1H Ar), 6.49 (s, 1H Ar), 7.58-7.89 (m, 4H Ar), 10.44 (s, 1H, NH). 3-(7-bromo-6,8-dimethoxy-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 28h
By replacing 3-(2-amino-4,5-dimethoxybenzoyl)benzonitrile 2a in example 3a by 3-(6- amino-3-bromo-2,4-dimethoxybenzoyl)benzonitrile 27h and proceeding in the same manner, the above product was obtained. Yield : 24%. 'H NMR (DMSO, 200 MHz) : d 3.32 (s, 2H, NCH;), 3.61 (s, 3H, OCHj3), 3.98 (s, 3H, OCH3), 6.41 (s, 1H, 1H Ar), 7.49-8.16 (m, 4H Ar), 9.80 (s, 1H, NH). 3-(8-methoxy-1-methyl-2-o0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 29a
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4k by 3-(8-methoxy-2-0xo0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzonitrile 28a and proceeding in the same manner, the above product was obtained. Yield : 57%. 'H NMR (CDCl, 200 MHz) : d 3.40 (s, 3H, NCH;), 3.91 (s, 3H, OCH3), 4.30 (AB system, ?d = 1.00 Jog = 16 Hz, 2H, CH,), 6.74-6.84 (m, 2H Ar), 7.13-7.18 (d, 1H Ar), 7.48-7.53 (t, 1H Ar), 7.70-7.74 (d, 1H Ar), 7.88 (m, 2H
Ar). 3-(6,8-dimethoxy-1-methyl-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 29b
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4a by 3-(6,8-dimethoxy-2-0x0-2,3-dihydro-1H-14- benzodiazepin-5-yl)benzonitrile 28g and proceeding in the same manner, the above product was obtained. Yield : 77%. "H NMR (CDCl;, 300 MHz) : d 3.38 (s, 3H, NCH),
3.53 (s, 3H, OCH3), 3.93 (s, 3H, OCH), 4.35 (AB system, 7d = 1.00 Jog = 15 Hz, 2H,
CH,), 6.34 (s, 1H Ar), 6.47 (s, 1H Ar), 7.44-7.86 (m, 1H Ar). 3-(7-bromo-6,8-dimethoxy-1-methyl-2-0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5- yDbenzonitrile, 29¢
By replacing 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)- benzonitrile 3a in example 4a by 3-(7-bromo-6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzonitrile 28h and proceeding in the same manner, the above product was obtained. Yield : 20%. 'H NMR (CDCl, 300 MHz) : d 3.29 (s, 3H, NCH), 3.49 (s, 3H, OCH3), 3.94 (s, 3H, OCH3), 4.37 (broad s, 2H, CH), 6.25 (s, 1H Ar),.7.44- 7.75 (m, 4H Ar).
Synthetic route of substituted phenyl meta carboxamides of type 36. 3-(2-amino-4-hydroxy-5-methoxybenzoyl)benzoic acid, 32a
A mixture of 1.5 g (5.31 mmoles) of (2-amino-3,4-dimethoxybenzoyl)benzonitrile 2a, 3.13 g (55.8 mmoles) of KOH in 25 ml of ethylene glycol was heated overnight at 140°C, then 150 ml of ice water were added. 0.1 N HCI was added to obtain pH 3-4.
The mixture was extracted with 4 x 150 ml of EtOAc and dried on MgSO,. The EtOAc was evaporated and the product was purified by chromatography on silica gel (EtOAc).
Yield : 70%. "H NMR (CDCl;, 300 MHz) : d 3.68 (s, 3H, OCH3), 6.31 (s, 1H Ar), 6.82 (s, 1H Ar), 7.57-8.34 (m, 4H Ar). Mass : (M + H)" = 288.07. 3-(7-methoxy-8-hydroxy-2-oxo-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzoic acid, 33a
A mixture of 300 mg (1 mmole) of 3-(2-amino-4-hydroxy-5-methoxybenzoyl)benzoic acid 32a, 150 mg (2 mmoles) of ethyl glycinate. HCI, and 5 ml of anhydrous pyridine was heated under reflux under an inert atmosphere for 36 hours. Four 100 mg fractions (0.79 mmol) of ethyl glycinate. HCI were added every 6 hours. The reaction was brought to room temperature and evaporated to dryness. 100 ml of ice water were added and the solution was filtered then washed with cold water, with EtOH, then with Et,O, and dried.
Yield : 45%. The product was used in the next reaction without further purification. 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydre-1H-1,4-benzodiazepin-5-yl)methyl benzoate, 34a 390 mg (9.6 mmoles) of 60% NaH in oil were added at 0°C in an inert atmosphere to a solution of 1 g (3.2 mmoles) of 3-(7-methoxy-8-hydroxy-2-oxo-2,3-dihydro-1H-1,4- benzodiazepin-5-yl) benzoic acid 33a in 10 ml of DMF and stirred at room temperature for 1 hour. At 0°C, 600 ul of iodomethane were added dropwise and the reaction was stirred overnight at room temperature. 200 m! of water were then added and the reaction was extracted with 3 x 200 ml of EtOAc and dried on MgSO4. The EtOAc was evaporated and the product was purified by chromatography on silica gel (EtOAc, then
EtOAc /CH,Cly/EtOH, 5:4:1). Yield : 50%.. 'H NMR (CDCls, 300 MHz) : d 3.42 (s, 3H,
NCH3), 3.74 (s, 3H, OCH3), 3.93 (s, 3H, COOCH3;), 4.00 (s, 3H, OCH3), 4.33 (AB system, 7d = 1.01, Jag = 8.3, 2H, CH), 6.65 (s, 1H Ar), 6.81 (s, 1H Ar), 7.49-8.27 (m, 4H Ar). Mass : (M + H)" = 369.1. 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydre-1H-1,4-benzodiazeepin-5-yl)benzoic acid, 35a 300 mg of KOH pellets were added at 0°C to a solution of 1.8 g (4.8 mmoles) of 3-(7,8- dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) methyl benzoate 34a in 75 ml of MeOH and 25 ml of water. The solution was heated at 60°C for 1 hour.
The methanol was evaporated, 100 ml of ice water were added and the solution was then acidified to pH 2-3 by dropwise addition of IN HCl. The solution was extracted with 4 x 150 ml of EtOAc and dried on MgSQO4. The EtOAc was evaporated. Yield 50%. 'H
NMR (CDCl;, 300 MHz): d 3.44 (s, 3H, NCH3), 3.75 (s, 3H, OCHj3), 4.00 (s, 3H,
OCHj3), 4.37 (AB system, 7d = 1.07, Jag = 10.9, 2H, CH), 6.66 (s, 1H Ar), 6.81 (s, lH
Ar), 7.50-8.39 (m, 4H Ar). Mass : (M + H)" = 369.1.
3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)N- isopropylbenzamide, 36a
To a solution of 100 mg (0.28 mmole) of 3-(7,8-dimethoxy-1-methyl-1,3-dihydro-2H- 1,4-benzodiazepin-5-yl)benzoic acid 35a and 17 mg (0.29 mmole) of isopropylamine in 4 ml of DMF, 117 mg (1.17 mmoles) of N-methylmorpholine were added followed by 192 mg (0.43 mmole) of BOP. The mixture was stirred overnight at room temperature. 100 ml of water were added and the solution was extracted with 3 x 100 ml of CHCl, and dried on MgSO4. The CH,Cl, was evaporated and the product was purified by chromatography on silica gel (EtOAc/hexane, 3:1 followed by EtOAc). Yield : 75%. 'H
NMR (DMSO, 300 MHz): d 1.27 (d, J,» = 6.5, 6H, CH(CHa)3), 3.40 (s, 3H, NCH3), 3.72 (s, 3H, OCH3), 3.98 (s, 3H, OCHj), 4.10 (m, J»; = 6.5, J23 = 7.2, 1H, CH), 4.32 (AB system, 7d = 0.99, Jag = 10.3, 2H, CH), 6.20 (d, J32 =7.2, 1H exchangeable, ;prNH), 6.63 (s, 1H Ar), 6.78 (s, 1H Ar), 7.42-8.08 (m, 4H Ar). Mass : (M+H)" = 396.16.
N-benzyl-3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) benzamide, 36b
By replacing isopropylamine in example 36a by benzylamine and proceeding in the same manner, the above product was obtained. Yield : 80%. 'H NMR (CDCls, 300
MHz) : d 3.35 (s, 3H, NCH3), 3.69 (s, 3H, OCH3), 3.95 (s, 3H, OCH3;), 4.23 (AB system, ?d = 0.97, Jag = 10.3, 2H, CH3), 4.60 (m, 2H, PhCH3), 6.60 (s, 1H Ar), 6.76 (s, 1H Ar), 7.15 (m, 1H exchangeable, BnNH), 7.27-8.12 (m, 9H Ar). Mass : (M+H)" = 444.20.
N-(6-amino-6-oxohexyl)-3-(7,8-dimethoxy-1-methyl-2-0xo0-2,3-dihydro-1H-1,4- benzodiazepin-5-yl)benzamide, 36¢
To a solution of 100 mg (0.28 mmole) of 3-(7,8-dimethoxy-1-methyl-1,3-dihydro-2H- 1,4-benzodiazepin-5-yl)benzoic acid 35a and 48 mg (0.29 mmole) of 5- aminopentylcarboxamide hydrochloride in 4 ml of DMF, 30 mg of triethylamine, 117 mg (1.17 mmoles) of N-methylmorpholine were added followed by 192 mg (0.43 mmole) of BOP. The reaction was stirred overnight at room temperature. 100 ml of water were added and the reaction was extracted with 3 x 100 ml of CH,Cl,, then dried on MgSO,. The CH,Cl, was evaporated and the product was purified by chromatography on silica gel (EtOAc/CH,Cl/EtOH, 5:4:1).Yield : 75%. 'H NMR (CDCls, 200 MHz) : d 1.66-1.73 (m, 6H, (CH3)3), 2.28 (m, 2H, COCH,), 3.44-3.51 (m, 5H, NHCH, + NCH3), 3.77 (s, 3H, OCH}), 4.02 (s, 3H, OCH3), 4.33 (AB system, 7d = 0.97, Jag = 10.5, 2H, CH,), 5.70 (broad s, 2H exchangeable, CONH,), 6.69 (m, 2H,
CONH + 1H Ar), 6.82 (s, 1H Ar), 7.45-8.20 (m, 4H Ar). Mass : (M+H)" = 467.2. 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-N,N- dimethylbenzamide, 36d
By replacing isopropylamine in example 36a by dimethylamine and proceeding in the same manner, the above product was obtained. Yield : 90%. 'H NMR (CDCl, 300
MHz) :d 3.01 (s, 3H, N(CHas),), 3.13 (s, 3H, N(CHj3)z), 3.43 (s, 3H, NCH3), 3.77-3.85 (m, 4H, 1HCH, + OCH), 4.01 (s, 3H, OCHj3), 4.82 (m, 1HCH3,), 6.70 (s, 1H Ar), 6.81 (s, 1H Ar), 7.46-7.77 (m, 4H Ar). Mass : (M+H)" = 382.20. 5-{3-[(4-benzylpyperazin-1-yl)carbonyl]phenyl}7,8-dimethoxy-1-methyl-2-0xo0-2,3- dihydro-1H-1,4-benzodiazepin-2-one, 36e
By replacing isopropylamine in example 36a by N-benzylpiperazine and proceeding in the same manner, the above product was obtained. Yield : 70%. 'H NMR (CDCls, 300
MHz) : d 2.43-2.71 (m, 4H, 2CH:pyp), 3.43-3.57 (m, 9H, PhCH; + 2CH;pyp + NCH), 3.77-3.85 (m, 4H, 1HCH; + OCHj3), 4.01 (s, 3H, OCH3), 4.83 (m, 1HCH2), 6.70 (s, 1H
Ar), 6.81 (s, 1H Ar), 7.30-7.77 (m, 9H Ar). Mass : (M+H)" = 513.20. 3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1 H-1,4-benzodiazepin-5-yl)-N-(3- phenylpropyl)benzamide, 36f
By replacing isopropylamine in example 36a by 3-phenylpropylamine and proceeding in the same manner, the above product was obtained. Yield : 95%. 'H NMR (CDCl3, 300
MHz) : d 1.97-2.04 (m, 2H, PhCH3), 2.72-2.80 (m, 2H, CH), 3.44-3.53 (m, SH, NHCH,
+ NCH3), 3.76-3.86 (m, 4H, 1HCH, + OCHj3), 4.01 (s, 3H, OCH3), 4.83 (m, 1HCHD>), 6.28 (broad s, 1H exchangeable, CONH), 6.66 (s, 1H Ar), 6.82 (s, 1H Ar), 7.26-8.06 (m, 4H Ar). Mass : (M+H)" = 472.20. - Synthesis of boronics 37 not commercially available 2-hydroxy-5-iodobenzonitrile, 37a
To a solution of 2 g (16.8 mmoles) of 2-hydroxybenzonitrile in 50 ml of acetonitrile under an inert atmosphere at -20°C, 1.65 ml of trifluoromethane sulfonic acid were added followed by incremental addition of 4.5 g (20.2 mmoles) of N-iodosuccinimide.
The reaction was stirred at room temperature for 12 hours, then 200 ml of water were added and the reaction was extracted with 3 x 150 ml of CH,Cl; and dried on MgSOs;.
The CHCl, was evaporated and the product was purified by chromatography on silica gel (EtOAc/hexane, 1:4). Yield : 85%. '"H NMR (CDCl;, 200 MHz) : d 6.78 (m, 1H Ar), 7.70-7.79 (m, 2H Ar), 8.17 (m, 1H exchangeable, OH). 5-iodo-2-[(4-methoxybenzyl)oxy] benzonitrile, 37b
A mixture of 2 g (8.16 mmoles) of 2-hydroxy-5-iodobenzonitrile 37a, 4.5 g (32.64 mmoles) of K>CO;3, 295 mg (0.8 mmole) of tetra n-butyl ammonium iodide, 1.4 g (8.98 mmoles) of 4-methoxybenzylchloride in 75 ml of anhydrous acetone was stirred at room temperature under an inert atmosphere for 12 hours. The acetone was evaporated, 150 ml of water were added and the solution was extracted with 3 x 150 ml of CH,Cl, and dried on MgSO4. The CHCl, was evaporated, the residue was triturated in 20 ml of
EtOAc, filtered, rinsed with a minimum of EtOAc, and dried. Yield : 75%. 'H NMR (CDCl;, 300 MHz) : d 3.81 (s, 3H, OCH3), 5.13 (s, 2H, CH,Ph), 6.79 (m, 1H Ar), 6.92 (m, 2HBn), 7.35 (m, 2HBn), 7.72-7.82 (m, 2H Ar). 3-cyano-4-[(4-methoxybenzyl)oxy]phenylboronic acid, 37¢ 1 ml of 1.7 M terBuLli in pentane cooled to -78°C was added by cannulation at —78°C under an inert atmosphere to a solution of 300 mg (0.82 mmole) of 5-iodo-2-[(4-
methoxybenzyl)oxy]benzonitrile 37b in 10 ml of anhydrous THF. The reaction was stirred for 30 minutes, then a solution of 930 pl of trimethylborate in 10 ml of anhydrous
THF cooled to -78°C was added by cannulation. The solution was allowed to return to : room temperature overnight. 50 ml of ice water were added and the solution was extracted with 4 x 50 ml of EtOAc and dried on MgSO,;. The EtOAc was evaporated and the product purified by flash chromatography on silica (EtOAc, then
EtOAc/CH,Cl/EtOH 5:4:1). Yield : 65%.
EXAMPLE 2 : PHARMACOLOGICAL ACTIVITY : INHIBITION OF
PHOSPHODIESTERASES. 2.1. Isolation of phosphodiesterases from smooth muscle
A 3 g segment of bovine aortic media cut into pieces with scissors was homogenized with an ultra-turrax then a potter glass/glass homogenizer in 7 volumes by weight of buffer A containing a protease inhibitor cocktail (20 mM Tris-HCI, 0.25 M saccharose, 2 mM magnesium acetate, | mM dithiothreitol, 5 mM EGTA, 2000 U/ml aprotinin, 10 mg/l leupeptin and 10 mg/l soya trypsic inhibitor). The homogenizate was centrifuged at 105,000 g for 1 hour. The supernatant was loaded on a DEAE-Sephacel column (15 x 1.6 cm) pre-equilibrated with buffer B (buffer A without the saccharose, EGTA and protease inhibitors). The column was washed until there was no detectable absorption at 280 nm, then eluted with a linear gradient of NaCl (0-0.5 M) in buffer B. 3-ml fractions were collected and enzyme activity was determined under the conditions described hereinbelow to localize the different enzymes PDE1, PDE3, PDE4 and PDES which were aliquoted and frozen at —80°C (Lugnier et al., Biochem. Phamacol., 1986, 35: 1746-1751). PDE2 was prepared from bovine endothelial cells by the same methods (Lugnier and Schini, Biochem. Pharmacol., 1990, 39: 75-84). 2.2. Protocol for measuring phosphodiesterase activity
Cyclic nucleotide phosphodiesterase activity was determined by a radioenzymatic method using tritium-labelled cyclic GMP or AMP (1 pM) as substrate (Lugnier et al.
1986). *H-labelled adenosine or guanosine monophosphate formed by hydrolysis of the radiolabelled cyclic nucleotide was then converted to 3H-labelled adenosine or guanosine in a second reaction with one nucleotidase in excess. The nucleoside formed was separated from the nucleotides by anion exchange chromatography. Nucleoside radioactivity was determined by liquid scintillation counting. Enzymatic incubations were carried out under conditions allowing no more than 15 % hydrolysis of the substrate; each point was performed in duplicate. 2.2.1. Determination of inhibition of PDE2.
The concentration of substance which inhibits enzymatic activity by 50 % (ICso) at 1 uM cyclic AMP was calculated by nonlinear regression from the experimental values of hydrolysis rate (Prism, GraphPad). 2.2.2. Selectivity
The activity of the compounds was evaluated on other phosphodiesterase isoforms, particularly basal state or calmodulin-activated PDE] from vascular smooth muscle,
PDE3, PDE4 and PDES from vascular smooth muscle.
The results obtained are presented in Tables 1 and 2 hereinbelow and are expressed as the percentage inhibition of enzymatic activity produced by 10 pmol of the test compound.
Table 1
Compound represented by formula (I comnat | | co ercentage ercentage
Compound inhi lton at M Compound hibition at M 3% | 22 | 6 | om 4 | er | 6 | 775% 4c | 3B3% | em [| 823% 4d | 476% | en | 846% de | 19% | 60 | = 793%
ERT 27 4 | w3% | 7 | 55 4 | 56% | 8 | 9 4 | 724% | 9a | = 85% 4m | 15 | 9 | = 914% 4 | 37 4 | 1 1 1 4 | 3% 4 | 34% | tod | = 55% | 15 | ub | = 693% 5s | 21 | tea | 16% 5 | 83% 5d | 192% | tb | 15
Se | 66 | t7¢ | er 5f | te | 7d |] 5¢ | 246 | 17e | 67 5 0% | i 5) | 45 | t7zh | 92% 5 | ws | wp | 70 5m | 59% | tk | 37 6&0 | | wm | 50 5% | | wm | 48 6a | 84 | azn | 38% 6b | 106 | 170 | 78 6c | 43 | 2b | = 38%
cos | pn | ones | rat
Compound percentage Compound percentage inhibition at 10 uM inhibition at 10 uM ed | 24 | 23% | 204% 6e | 03% | 23d 6h | | 24 [ 33 6 | 56 | 26a | 0% 28b | 229% | 28 25d | 24% | 20 2% | 50 | 2¢ 28b | 345%88 | 3b | 31 28d | 47% | 36d | 17% 28
Table 2
Selectivity inhibition at 10M
PDE1 | PDE2 | PDE3 | PDE4 | PDE5S 4m | a5 | |] 4p | 25% | 18 | 58% | 19% | 26% ba | 132% [| 15 | 5% | 162% | 17.6% sb | [lat [| 216% eb | Jtoe 6d | | 24 | [857% 6e | Jos 6 | Jon | 5 | 28 6m | |823%| | 373% 6n | [846% | | 587% 7a | | 313] | 652 9 | joan] 176 [| 10% [ 15 | 36% [ 8% | 14%
All the compounds tested showed potent inhibition of PDE2. The preferred compounds according to the invention have an excellent potency and selectivity profile for phosphodiesterase 2, in so far as said compounds are weaker inhibitors of the other
PDEs, particularly PDE3.
EXAMPLE 3 : BEHAVIORAL TESTS
Compound Sa was evaluated in different behavioral tests 3.1 Elevated plus maze test
This test was validated in the rat by Pellow in 1986 and in the mouse by Lister in 1987. It is based on an aversion to open spaces: the open arms elicit anxiety in the animals while the closed arms represent safety. By recording the frequency of entry into each arm, this test evaluates the anxiolytic effect of a molecule in comparison with a reference compound such as buspirone.
Ten to eleven-week old Balb/c or Swiss mice were used for the test. Mice were randomly divided into a control group (treated with the vehicle) and other groups treated with the test compounds.
The test apparatus was a PVC maze with a plexiglass lid, divided into four equivalent exploration arms (45 x 10 cm), all interconnected by a small platform (10 x 10 cm). The apparatus was placed 66 cm above the floor. Two arms were opened and the other two closed with a wall (height : 30 cm).
After administration, the mouse was placed on the platform opposite the closed arm.
The number of entries and the time spent in each open arm were recorded over § minutes.
The treatment was administered 1 hour before the test. The compounds were given orally at different doses. The results are shown in Figures 8 and 9. N=10; *** p< 0.005 and *¥*** p<0.001 (versus control; Dunnett test).
A significant difference between the groups was observed, in particular with regard to the percentage of time spent in the open arms. Mice treated with compound 5a spent more time in the open arms, and this at all doses tested. 3.2 Swim test
This test is based on the induction of alternative behavior in rodents subjected to an acute stress. In this model, the rat or mouse placed in a water-filled cylinder adopts a particular state of immobility. Onset of said immobility is delayed by antidepressants administered acutely or in repeated doses.
Wistar rats or Swiss mice were used. Animals were isolated for one week with a reverse light/dark cycle, then placed in the water-filled cylinder for 6 minutes.
The total immobility time was recorded during the last 4 minutes.
The treatment was administered 20 minutes before the test. Four groups were used to test three different doses : one control group treated with the vehicle, and one group for the three doses.
The results are shown in Figures 10 and 11. Two parameters were recorded : onset of immobility and immobility time. N= 10 ; *¥** p<0.005 (Dunnett test).
The statistical analyses showed a significant difference between the groups for total immobility time (p = 0.007). Mice treated with compound 5a at 3 and 30 mg/kg had a shorter immobility time than the control group and the group treated with compound 5a at 0.3 mg/kg. 3.3 Light/dark test
This light/dark test is based on the natural tendency of rodents to prefer a dark environment and thereby evaluates the emotional responses of the animals in a situation of bright light. This procedure is suitable for assessing the state of anxiety elicited by anxiety stimuli (Lister’s group, 1990). Mice placed in the apparatus, while showing a preference for the dark zone, nonetheless explore the light zone. This procedure was validated in 1990 by Misslin et al., who demonstrated that the anxiolytic and angiogenic properties of different substances act on the serotoninergic system or on benzodiazepine
GABA receptor complexes.
Ten to eleven-week old Balb/c or Swiss mice were used. Mice were randomly divided into a control group (treated with the vehicle) and other groups treated with the test compounds.
The test apparatus consisted of two PVC compartments (20 x 20 x 14 cm) with a plexiglass lid. One of the compartments was dark. A 100W light bulb was placed 15 cm above the other compartment, emitting the only light in the room (approximately 4400 lux). An opaque plastic tunnel separated the light and dark compartments.
The animal was placed in the light compartment with its head pointed towards the tunnel. The time spent in the light compartment and the number of entries into the light compartment were recorded for 5 minutes after the first entry into the dark zone. The test compound or the control treatment were administered orally 1 hour before the test.
The results are shown in Figures 12 and 13. N = 10; ** p<0.01 ; **** p<0.001 (Dunnett test versus control).
A significant difference was observed between the groups for the time spent in the light compartment (p<0.001). Mice treated with compound 5a at 0.3, 3 and 30 mg/kg spent significantly more time in the light compartment as compared with controls (p<0.01, control versus Dunnett test).
Together these results confirm the anxiolytic and antidepressant effect of the inventive compounds and in particular of compound Sa, in particular at the doses tested.

Claims (46)

1. Use of at least one phosphodiesterase 2 inhibitor for preparing a pharmaceutical composition for treating pathologies related to the central or peripheral nervous system.
2. Use according to claim 1, wherien the nervous system is central nervous system.
3. Use according to the claim 1 or 2, wherein the pathologies are due to a deregulation of the function of a neurotransmitter or to a deficiency in the release of a neurotransmitter;
4. Use according to claim 3, wherein the neurotransmitter is dopamine.
5. Use according to any one of the previous claims, wherein the pathologies are selected in the group consisting of depression, schizophrenia, anxiety, bipolar disorder, attention I5 deficit disorders, sleep disorders, obsessive compulsive disorder, fibromyalgia, Tourette's syndrome, pharmacodependence, epilepsy, Alzheimer’s disease, Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, obesity and Lewy body dementia.
6. Use according to claim 1 or 2, wherein the pathologies involve the peripheral nervous system and the peripheral organs.
7. Use according to claim 6, wherein the pathologies are of the type reduced natriuria, acute renal failure, hepatic dysfunction, or acute hepatic failure.
8. Use according to claim 6 or 7, wherien the pathologies are due to age.
9. Use according to claim 1 or 2, wherein the pathologies are due to or involve dysfunctions of prolactin secretion AMENDED SHEET
10. Use according to claim 9, wherein the pathologies are selected from the group consisting of restless legs syndrome, rheumatismal, and allergic or auto-inflammatory disorders.
11. Use according to claim 9 or 10, wherien the pathologies are selected from the group consisting of rheumatoid arthritis, rhinitis and asthma.
12. Use according to claim 1, wherein the pathologies are selected in the group consisting of anxiety, depression and schizophrenia.
13. Use according to any one of the previous claims, wherein the PDE2 inhibitors are selected in the group consisting of 1,4-benzodiazepine derivatives.
14. Compounds represented by general formula (I): Rq Ri z R N R; —N Rj Rs R; i) in which : . Z represents an oxygen or sulfur atom or a NR; group,
. Ry is the hydrogen atom, a (C,-Cq) alkyl group, a (Cs-Cig)aryl group or a (C;- Cs)alkyl(Cs-Cig)aryl or (Cs-Cy5)aryl(C;-Ca)alkyl group, . Ry is a hydrogen atom, a (C,-C¢) alkyl group, a (Ce-Cis)aryl group or a (Ci- Cg)alkyl(Ce-Cig)aryl or (Cs-Cg)aryl(C;-Ca)alkyl group, AMENDED SHEET
R; and R; taken together can optionally form a linear or branched hydrocarbon chain having from 2 to 6 carbon atoms, possibly containing one or several other double bonds and/or possibly interrupted by an oxygen, sulfur or nitrogen atom,
5. Rj and Ry, which are the same or different, represent the hydrogen atom, a (C;-Cy3) alkyl, (C3-Cs) cycloalkyl, (Cg-Cyg) aryl, (Ce-Cig)aryl(C-Cylalkyl, (C;-Cjr)alkyl(Cs- Cig)aryl group or a (Cs-Cg) heterocycle, aromatic or not, containing 1 to 3 heteroatoms, a NO», CF3;, CN, NR'R"’, SR’, OR’, COOR’, CONR’R"* or NHCOR'R"’ group, R’ and R", independently of each another, being selected in the group consisting of the hydrogen atom, a (C;-Cs) alkyl, (C3-Cs) cycloalkyl, (Cs-Cy,) aryl group and a (Cs-C3) heterocycle, aromatic or not, containing ! to 3 heteroatoms;
. Rs represents a phenyl group substituted at least in position 3, a naphthyl group, a (Cs- Cis) heterocycle, aromatic or not, containing ! to 3 heteroatoms, selected in the group consisting of the pyridyl, isoquinolyl, quinolyl and piperazinyl group, provided that when Rs is a naphthyl group substituted in position 6, then the latter is not attached to the rest of the molecule in position 2, or when Rs is a pyridyl group, then it is not attached to the rest of the molecule in position 4, or when Rj; is a tetrahydro 1,2,3,4- isoquinolyl group, then it is not attached to the rest of the molecule in position 2, when Rs represents a phenyl group substituted at least in position 3, said substituent being selected in the group consisting of : an alkyl, halogenoalkyl, cycloalkyl, alkenyl, alkynyl, aralkyl, aryl, heterocycle, heterocycloalkyl group, a OH, =O, NO,, NH,, CN, CF;, COR’, COOR’, (Ci-Cg)alkoxy, (di)(Ci-Ce)alkylamino, NHCOR’, CONR’R”’ group, in which R’ and R’’ are such as defined hereinabove, CHO, CONH2, phenyl optionally substituted, by an acetyl group, by a halogen atom (Cl), by a CONH2 group or by a CN, prop-1-ynyl optionally substituted, by a benzyloxy or tert-butyl carbamate, hex-1-ynyl optionally substituted, by a CN or NH2, pentyl optionally substituted, by a CONH2, hexyl, piperidinyl group optionally substituted, by a prop-1-ynyl, benzylaminomethyl, acetamide (CH3CONH), aminomethyl, NH2CS-, 4-phenyl-1, 3- thiazol-2-yl, -CONHBenzyl, -COOEthyl, -CONHiPropyl, -CONH-(CH2)n-CONH2 (n representing a whole number from 1 to 6), -CONR’R’’ group, with R’ and R”’, which are the same or AMENDED SHEET different, representing a C1-C6 alkyl group or a hydrogen atom, -(4-benzylpyperazin-1- yl)carbonyl, -CONH-(CH2)n-phenyl (n representing a whole number from 1 to 6), imidazolyl, piperazinyl optionally substituted, by a phenyl group,
5. R; and Rs, independently of each other, are selected in the group consisting of the hydrogen atom, a halogen atom or a OR, group in which R;, represents a hydrogen atom, a (C,-Cq) alkyl, (C;-C) cycloalkyl, (Ce-C;2) aryl group, or a (Cs-C),) heterocycle, aromatic or not, containing 1 to 3 heteroatoms, at least one of the groups R; and Rg representing a OR group such as defined hereinabove,
. Rg and Ry, independently of each other, are selected in the group consisting of the hydrogen atom, a halogen atom, an alkyl, cycloalkyl, alkenyl, alkynyl group, an aryl, aralkyl, heterocycle, heterocycloalkyl group and a OR group, R;o being such as defined hereinabove, the alkyl, cycloalkyl, alkenyl, alkynyl, aralkyl, aryl, phenyl, naphthyl, heterocycle, heterocycloalkyl group or the hydrocarbon chain defined hereinabove being optionally substituted by one or more substituents, which are the same or different, and which can be selected in the group consisting of a halogen atom, an alkyl, halogenoalkyl, cycloalkyl, alkenyl, alkynyl, aralkyl, aryl, heterocycle, heterocycloalkyl group, a OH, =0, NO, NH;, CN, CF; COR’, COOR’, (C;-Ce)alkoxy, (di)(C,-Cs)alkylamino, NHCOR’ and CONR'R"’ group, in which R’ and R’’ are such as defined hereinabove, the substitutents also being optionally substituted, and the salts of compounds represented by formula (I), with the exception of compounds represented by formula (I) in which . - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, RS is a phenyl group substituted at least in position 3 by a methoxy group, - Rl is an alkyl group or a hydrogen atom, R3 and R’3 are hydrogen atoms, R6 and R9Y are hydrogen atoms, RS is a phenyl group substituted only in position 3 by a chlorine or bromine atom, AMENDED SHEET
- Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, RS is a phenyl group substituted at least in position 3 by a CH2OH group, - Rl is a hydrogen atom, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, R5 is a phenyl group substituted only in position 3 by a CF3 group, - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, RS is a phenyl group substituted in positions 3 and 5 by a CF3 group, - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, R7 and R8 are methoxy groups, RS is a phenyl group substituted in positions 3 by a phenyl group, - Rl is an alkyl group, R3 and R’3 are hydrogen atoms, R6 and R9 are hydrogen atoms, R7 and R8 are methoxy groups, R5 is a phenyl group substituted in positions 3 by a phenylethynyl group.
15. Compounds represented by formula (I) according to claim 14, in which Rs is a phenyl group substituted at least in position 3.
16. Compounds represented by formula (I) according to the previous claim, in which the substituents can be selected in the group consisting of : CHO, CN, CONH2, NO2, CF3, NH2, halogen atom (Cl), (C1-C6) alkyl, phenyl optionally substituted, by an acetyl group, by a halogen atom (Cl), by a CONH2 group or by a CN, prop-1-ynyl group optionally substituted, by a benzyloxy or tert-butyl carbamate, hex-1-ynyl group optionally substituted, by a CN or NH2 group, pentyl optionally substituted, by a CONH2, hexyl, piperidinyl optionally substituted, by a prop-1-ynyl, benzylaminomethyl, acetamide (CH3CONH), aminomethyl, NH2CS-, 4-phenyl-1, 3- thiazol-2-yl, -CONHBenzyl, -COOEthyl, -CONHiPropyl, -CONH-(CH2)n-CONH2 (n representing a whole number from 1 to 6), -CONR’R”’ group, with R’ and R’’, which are the same or different, representing a C1 to C6 alkyl group or a hydrogen atom, -(4- benzylpyperazin-1-yl)carbonyl, -CONH-(CH2)n-phenyl (n representing a whole number from 1 to 6), imidazolyl, piperaziny! group optionally substituted, by a phenyl group.
17. Compounds represented by formula (I) according to any one of claims 14 to 16, in which RS is a phenyl group substituted in positions 3 and 4, by a AMENDED SHEET hydrocarbon chain possibly containing at least one heteroatom, such as oxygen, like the methylenedioxy (-O-CH2-0-) chain forming a ring with the phenyl group to which it is attached.
18. Compounds represented by formula (I) according to claim 14, in which RS is the 3- pyridyl, 4-isoquinolyl, piperazinyl group optionally substituted, in position 4 by an aryl group, such as phenyl.
19. Compounds represented by formula (I) according to claim 14, in which Z represents a sulfur atom or -NR2, with in particular R2 forming a ring of the imidazole type with
RI.
20. Compounds represented by formula (I) according to any one of claims 14, 15 or 16, in which : - Zs the oxygen atom, and/or - Ry and Rg, independently of each other, represent a ORq group in which Ry is a (C,-Cg) alkyl group, such as an ethyl or methyl group, - Rj; and Rj each represent an ethoxy or methoxy group, or one represents a hydrogen atom and the other an ethoxy or methoxy group, and/or - Rg and Ry, which are the same or different, represent the hydrogen atom, a halogen atom, a phenyl group, a (C;-Ce) alkyl group or a OR} group in which Rjois a (C;-Cs) alkyl group, such as an ethyl or methyl group, and/or - Rj and Ry’, which are the same or different, represent a hydrogen atom, and/or - Ry is a (C1-C6) alkyl, (Cs-Cys) aryl group, such as phenyl, (C-Cig)aryl(C)- Cy)alkyl group such as benzyl optionally substituted, or (C,-C);)alkyl(Ce- Cig)aryl group.
21. Compounds represented by formula (I) according to any one of claims 14 to 20, in which R, represents a hydrogen atom or a (C,-Cs) alkyl, (Cs-C,3) aryl (for AMENDED SHEET example : phenyt), (C¢-Cs)aryl(C,-Ca)alkyl (for example : benzyl), (Ci-C,2)alkyl(Cs- Cis)aryl group, said group being optionally substituted.
22. Compounds represented by formula (I) according to any one of claims 14 to 21, in which Rj; is a phenyl group substituted by: (h) one or more OR’ groups, such as methoxy or ethoxy, or (1) a COR’ group, such as acetyl or aldehyde, or 0) a CONR'R’’ group such as CONH2, or (k) a CN group, or 0) a trifluoromethyl group, or (m) an alkyl group, for example methyl, or alkynyl group, for example hexynyl or propynyl, or (n) an aryl or heterocycle group, such as phenyl, furyl, pyridyl, piperidine. thiazole or thienyl, said aryl or heterocycle itself being optionally substituted by one or more groups selected from groups (a)-(8).
23. Compounds represented by formula (I) according to any one of claims 14 to 22, in which at least one group Rj; or Rg represents OR; where Ryo represents a (C,-Ce) alkyl or (C;3-Cq) cycloalkyl group.
24. Compounds represented by formula (I) according to claim 23, in which at least one of the groups R7 and R8, both, represents a methoxy group.
25. Compounds selected from the following compounds: 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 3a 7,8-dimethoxy-[5-(3-trifluoromethyl)phenyl]-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 3d 3-(7,8-dimethoxy-1-methyl-2-oxo-2,3-dihydro- 1H-1,4-benzodiazepin-5-yl)-benzonitrile, 4a 3-{1-(4-chlorobenzyl)-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl]- benzonitrile, 4¢ AMENDED SHEET
3-(6-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sb 3-(7,8-dimethoxy-1-methyl-2-0x0-6-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sc
3-(9-bromo-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro- 1 H-1,4-benzodiazepin-5-
yl)benzamide, 5d 3-(7,8-dimethoxy-2-0x0-2,3-dihydro-1 H-1 J4-benzodiazepin-5-yl)benzamide, Se 3-(7,8-dimethoxy-1-propyl-2-oxo0-2,3-dihydro- 1H-1,4-benzodiazepin-5-yl)benzamide, Sf
3-(1-ethyl-7,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, Sg 3-(1-benzyl-7,8-dimethoxy-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, 5h ethyl {5-[3-(aminocarbonyl)phenyl]-7,8-dimethoxy-2-oxo-2,3-dihydro- 1H-1,4- benzodiazepin-1-yl}acetate, 5i
3-(7,8-dimethoxy-1,3-dimethyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) benzamide, 5j 3-[3-(3,4-dichlorobenzyl)-7,8-dimethoxy- 1 -methyl-2-0x0-2,3-dihydro-1H-1 4- benzodiazepin-5-yl]benzamide, 5k 3-(8-methoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, 51
3-(7,8-dimethoxy-1-methyl-2-ox0-9-phenyl-2,3-dihydro- 1 H-1,4-benzodiazepin- 5- yl)benzamide, Sm 3-(6.8-dimethoxy-2-ox0-2,3-dihydro-1 H-1,4-benzodiazepin-5-yl)benzamide, 5n 3-(6,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro- 1 H-1,4-benzodiazepin-5-yl)benzamide, So tert-butyl-3-[3-(7,8-dimethoxy- 1-methyl-2-o0x0-2,3-dihydro- 1 H-1,4-benzodiazepin-5- yl)phenyl]propynylcarbamate, 6a 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4-benzodiazepin- 2-one, 6b 7,8-dimethoxy-1-methyl-5-[3-(3-piperidin-1-ylprop-1-ynyl)phenyl]-1,3-dihydro-2H-1,4-
benzodiazepin-2-one, 6¢ 6-[3-(7,8-dimethoxy-1-methyl-2-oxo-2,3-dihydro- 1 H-1,4-benzodiazepin-5- yl)phenyl]hex-5-ynenitrile, 6d
AMENDED SHEET
7,8-dimethoxy-5-(3’-hexylphenyl)- 1-N-methyl-1,3-dihydro-2H-1 4-benzodiazepin-2- one, 6e 5-[3-(3-aminopropy!)phenyl-7,8-dimethoxy- 1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one trifluoroacetate, 6h
6-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyllhexanamide, 6i 5-(4'-chloro-1,1'-biphenyl-3-yl)-7,8-dimethoxy- 1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 6j 5-{3-[3-(benzyloxy)prop-1-ynyl]phenyl}-1-ethyl-7,8-dimethoxy-1,3-dihydro-2H-1,4-
benzodiazepin-2-one, 6k 3'-(7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-3-carbonitrile, 61 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carbonitrile, 6m
3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carboxamide, 6n 3'-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-3-carboxamide, 60 3-[3-(3,4-dichlorobenzyl)-7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-
benzodiazepin-5-yllbenzonitrile, 7b 7,8-dimethoxy-1,3-dimethyl-5-(3-trifluoromethylphenyl)-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 7¢ 3-(7,8-dimethoxy-1,3-dimethyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 7d
5-[3-(aminomethyl)phenyl]-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 8a N-[3-(7,8-dimcthoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzyl]acetamide, 8b 3-(7,8-dimethoxy- 1 -methyl-2-0x0-2,3-dihydro- 1H-1,4-benzodiazepin-5-
yDthiobenzamide, 9a 7,8-dimethoxy-1-methyl-5-[3-(4-phenyl-1,3-thiazol-2-yl)phenyl]-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 9b 5-(3-cyanophenyl)-7,8-dimethoxy-1,3-dihydro-2H-1,4-benzodiazepin-2-thione, 10d
AMENDED SHEET
3-(8,9-dimethoxy-4H-imidazo[1,2-a][1,4]benzodiazepin-6-yl)benzonitrile, 11a 3-(8,9-dimethoxy-4H-imidazo[ 1,2-a][1,4]benzodiazepin-6-yl)benzamide, 11b 3-(7,8-dimethoxy-2-methylamino-1,3-dihydro-3H-1,4-benzodiazepin-5-yl)benzonitrile, 12a
7,8-dimethoxy-1-methyl-5-(3-pyridyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17b 7,8-dimethoxy- 1-methyl-5-(3-nitrophenyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17¢ 5-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro- 1H-1,4-benzodiazepin-5-yl)-2- benzonitrile, 17d 5-(3-acetylphenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17e
5-(4-isoquinolinyl)-7,8-dimethoxy- 1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17f 7,8-dimethoxy-5-(3-hydroxymethylphenyl)-1-methyl-3-propyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 17h 5-(3-aminophenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 17i
5-(3.4-dichlorophenyl)-7,8-dimethoxy- 1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 17j 7,8-dimethoxy- 1-methyl-5-(3-methylphenyl)-1,3-dihydro- 1,4-benzodiazepin-2-one, 17k. 5-(3-formylphenyl)-7,8-dimethoxy-1-methyl-1,3-dihydro-1,4-benzodiazepin-2-one, 171 5-[3-(benzylaminomethyl)phenyl]-7,8-dimethoxy- 1-methyl-1,3-dihydro-2H-1,4-
benzodiazepin-2-one hydrochloride, 17m N-[3-(7,8-dimethoxy- 1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl) phenyl]acetamide, 17n 7,8-dimethoxy-1-methyl-5-(3,4-methylenedioxyphenyl)-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 170
3-(7-hydroxy-8-methoxy-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 22b 3-(6-bromo-7-hydroxy-8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 23b 3-(9-bromo-8-hydroxy-7-methoxy-2-0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5-
yl)benzonitrile, 23d 3-(6-bromo-7,8-dimethoxy-1-methyl-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 24b
AMENDED SHEET
3-(7,8-dimethoxy- 1-methyl-2-oxo-6-phenyl-2,3-dihydro- 1 H-1,4-benzodiazepin- 5- yl)benzonitrile, 25b 3-(7,8-dimethoxy- 1-methyl-2-0x0-9-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzonitrile, 25a tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4- benzodiazepin-9-yl)phenyllprop-2-ynylcarbamate, 25¢ methyl(2E)-3-[5-(cyanophenyl)-7,8-dimethoxy- 1-methyl-2-oxo0-2,3-dihydro- 14-1 4- benzodiazepin-9-yl)phenyl]acrulate, 25d tert-butyl-3-[5-(cyanophenyl)-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-
benzodiazepin-6-yl)phenyl]prop-2-ynylcarbamate, 25e [9-(3-aminoethynyl)-7,8-dimethoxy- 1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin- 5-yl]benzonitrile, 25f [6-(3-aminoethynyl)-7,8-dimethoxy-1-methyl-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin- 5-yllbenzonitrile, 25g
3-(8-methoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28a 3-(6-methoxy-2-0xo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28b 3-(7-methoxy-2-ox0-2,3-dihydro- 1 H- 1,4-benzodiazepin-5-yl)benzonitrile, 28¢ 6-methoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28d 7-methoxy-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one, 28e
9-bromo-7,8-dimethoxy-5-phenyl-1 ,3-dihydro-2H-1,4-benzodiazepin-2-one, 28f 3-(6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28g 3-(7-bromo-6,8-dimethoxy-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 28h 3-(8-methoxy-1-methyl-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 29a
3-(6,8-dimethoxy-1-methyl-2-o0xo0-2,3-dihydro- 1 H-1,4-benzodiazepin-5-yl)benzonitrile, 29b 3-(7-bromo-6,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro- | H-1,4-benzodiazepin-5- yl)benzonitrile, 29¢ 3-(7,8-dimethoxy-1-methyl-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)methyl benzoate, 34a 3-(7,8-dimethoxy-1-methyl-2-oxo-2,3-dihydro- 1 H-1,4-benzodiazepin-5-yl)benzoic acid, 35a
AMENDED SHEET
0) * 4 3-(7,8-dimethoxy- 1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-y1)N- isopropylbenzamide, 36a N-benzyl-3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1 H-1,4-benzodiazepin-5-yl) benzamide, 36b N-(6-amino-6-oxohexyl)-3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro- 1 H-1,4- benzodiazepin-5-yl)benzamide, 36¢ 3-(7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-N,N- dimethylbenzamide 36d 5-{3-[(4-benzylpyperazin-1-yl)carbonyl)phenyl} 7,8-dimethoxy-1-methyl-2-oxo0-2,3- dihydro-1H-1,4-benzodiazepin-2-one, 36e 3-(7,8-dimethoxy- 1-methyl-2-o0x0-2,3-dihydro-1H-1,4-benzodiazepin-5-y1)-N-(3- phenylpropyl)benzamide, 36f.
26. Compounds selected from the following compounds : 3-(1-benzyl-7,8-dimethoxy-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzonitrile, 4m 7,8-dimethoxy-1-methyl-[5-(3-trifluoromethyl)phenyl]-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 4p 3-(7,8-dimethoxy- 1-methyl-2-oxo0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)benzamide, Sa 3-(6-bromo-7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)benzamide, Sb tert-butyl-3-[3-(7,8-dimethoxy-1-methyl-2-0x0-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyl]propynylcarbamate, 6a 7,8-dimethoxy-5-(3’-hex-1-ynylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4-benzodiazepin- 2-one, 6b 6-[3-(7,8-dimethoxy- 1-methyl-2-oxo-2,3-dihydro-1H-1,4-benzodiazepin-5- yl)phenyl]hex-5-ynenitrile, 6d 7,8-dimethoxy-5-(3’-hexylphenyl)-1-N-methyl-1,3-dihydro-2H-1,4-benzodiazepin-2- one, 6e 5-(4'-chloro-1,1'-biphenyl-3-yl)-7,8-dimethoxy-1-methyl-1,3-dihydro-2H- 1 4- benzodiazepin-2-one, 6j AMENDED SHEET
—_— 3'-(7,8-dimethoxy- 1-methyl-2-0x0-2,3-dihydro- 1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carbonitrile, 6m 3'-(7,8-dimethoxy-1-methyl-2-ox0-2,3-dihydro-1H-1,4-benzodiazepin-5-yl)-1,1'- biphenyl-4-carboxamide, 6n 3-(3,4-dichlorobenzyl)-1-ethyl-7,8-dimethoxy-S-phenyl-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 7a 7,8-dimethoxy-1-methyl-5-[3-(4-phenyl-1, 3-thiazol-2-yl)phenyl]-1,3-dihydro-2H-1,4- benzodiazepin-2-one, 9b 7,8-dimethoxy-1-methyl-5-(3-pyridyl)-1,3-dihydro-1,4-benzodiazepin-2-one, 17b.
27. Pharmaceutical composition comprising at least one compound such as defined in any one of claims 14 to 26 and a pharmaceutically acceptable vehicle or excipient.
28. Use of a compound such as defined in any one of claims 14 to 26 for preparing a pharmaceutical composition for treating pathologies involving the central nervous system
29. Use of a compound according to claim 28, wherein the pathologies are due to a deregulation of the function of a neurotransmitter or to a deficiency in the release of a neurotransmitter.
30. Use according to the previous claim, wherein the pathology is selected in the group consisting of depression, schizophrenia, anxiety, bipolar disorder, attention deficit disorders, sleep disorders, obsessive compulsive disorder, fibromyalgia, Tourette’s syndrome, pharmacodependence, epilepsy, Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, multiple sclerosis, obesity and Lewy body dementia.
31. Use of a compound such as defined in any one of claims 14 to 24 for preparing a pharmaceutical composition for treating pathologies involving the peripheral nervous system and peripheral organs in general, and pathologies due to or involving dysfunctions of prolactin AMENDED SHEET
» ) ° [ secretion, such as restless legs syndrome, rheumatismal, allergic or auto-inflammatory disorders, such as rheumatoid arthritis, rhinitis and asthma.
32. Use of a compound according to claim 31, wherein the pathologies are of the type reduced natriuria, acute renal failure, hepatic dysfunction, or acute hepatic failure.
33. Use of a compound according to claim 31 or 32, wherien the patholgies are due to age.
34. Use of a compound such as defined in any one of claims 14 to 26 for preparing a pharmaceutical composition for treating disorders of the central or peripheral nervous system, of chronic or acute nature.
35. Use of a compound such as defined in any one of claims 14 to 26 for preparing a pharmaceutical composition for treating memory impairment or cognitive impairment.
36. Use of a compound according to claim 35, wherein the cognitive impairment is a mild cognitive impairment.
37. Use of a compound such as defined in any one of claims 14 to 26 for preparing a pharmaceutical composition for treating neurodegenerative diseases.
38. Use of a compound such as defined in any one of claims 14 to 26 for preparing a pharmaceutical composition for treating obesity.
39. Use of at least one phosphodiesterase 2 inhibitor as claimed in any one of claims 1 to 13, substantially as hereinbefore described or exemplified.
40. Use of at least one phosphodiesterase 2 inhibitor including any new and inventive integer or combination of integers, substantially as herein described. AMENDED SHEET
« to
41. Compounds of general formula (I) as claimed in any one of claims 14 to 24, specifically as hereinbefore described or exemplified and not claimed in claim 25 or 26.
42. Compounds of general formula (I) including any new and inventive integer or combination of integers, substantially as herein described.
43. Pharmaceutical composition as claimed in claim 27, substantially as hereinbefore described or exemplified.
44. Pharmaceutical composition according to the invention including any new and inventive integer or combination of integers, substantially as herein described.
45. Use of a compound of the formula (I) as claimed in any one of claims 28 to 38, substantially as hereinbefore described or exemplified.
46. Use of a compound of the formula (I) including any new and inventive integer or combination of integers, substantially as herein described. AMENDED SHEET
ZA200505412A 2002-10-30 2005-05-27 Cyclic nucleotide phosphodiestierase inhibitors, preperation and uses ZA200505412B (en)

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WO2020050722A1 (en) * 2018-09-05 2020-03-12 Universiteit Van Amsterdam Use of a pde11 or pde2 inhibitor for the treatment of parkinson's disease
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DE1136709B (en) * 1959-12-10 1962-09-20 Hoffmann La Roche Process for the preparation of 2-oxo-1, 2-dihydro-1, 4-benzodiazepines
DE1145626B (en) * 1959-12-10 1963-03-21 Hoffmann La Roche Process for the preparation of 3H-1, 4-Benzodiazepin-2 (1H) -one derivatives
US3515755A (en) * 1968-06-18 1970-06-02 Hoffmann La Roche Lower alkanoyl amido benzophenones
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DE1942744A1 (en) * 1969-08-22 1971-03-04 Cassella Farbwerke Mainkur Ag 2-imino-1,2-dihydro-3h-1,4-benzodiazepines
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