ZA200206918B - A process for generating genetically modified pearl millet through agrobacterium and biolistic transformation. - Google Patents

A process for generating genetically modified pearl millet through agrobacterium and biolistic transformation. Download PDF

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Publication number
ZA200206918B
ZA200206918B ZA200206918A ZA200206918A ZA200206918B ZA 200206918 B ZA200206918 B ZA 200206918B ZA 200206918 A ZA200206918 A ZA 200206918A ZA 200206918 A ZA200206918 A ZA 200206918A ZA 200206918 B ZA200206918 B ZA 200206918B
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South Africa
Prior art keywords
calli
gene
biolistic
days
media
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ZA200206918A
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English (en)
Inventor
Naganand Rayapuram
Divya Chandran
Ranjini Krishnan
Villoo Morawala Patell
Original Assignee
Avestha Gengraine Tech Pvt Ltd
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Publication date
Application filed by Avestha Gengraine Tech Pvt Ltd filed Critical Avestha Gengraine Tech Pvt Ltd
Publication of ZA200206918B publication Critical patent/ZA200206918B/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ZA200206918A 2000-02-25 2002-08-22 A process for generating genetically modified pearl millet through agrobacterium and biolistic transformation. ZA200206918B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN155CH2000 2000-02-25

Publications (1)

Publication Number Publication Date
ZA200206918B true ZA200206918B (en) 2004-03-30

Family

ID=11097162

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200206918A ZA200206918B (en) 2000-02-25 2002-08-22 A process for generating genetically modified pearl millet through agrobacterium and biolistic transformation.

Country Status (8)

Country Link
US (1) US20030140382A1 (fr)
EP (2) EP1261729A2 (fr)
JP (1) JP2004505605A (fr)
CN (1) CN1426476A (fr)
AU (1) AU5505001A (fr)
CA (1) CA2401247A1 (fr)
WO (1) WO2001062890A2 (fr)
ZA (1) ZA200206918B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8796508B2 (en) * 2005-11-10 2014-08-05 Pioneer Hi-Bred International, Inc. Microprojectile bombardment transformation of Brassica
US7750214B2 (en) * 2007-01-25 2010-07-06 Board Of Regents Of The University Of Nebraska Pearl millet line 53-1-1 with PP3 gene and all derivatives produced by any method
CN112941100B (zh) * 2021-03-04 2023-08-15 北京齐禾生科生物科技有限公司 一种中间偃麦草遗传转化方法及其专用引物

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ293737A (en) * 1994-07-29 1998-07-28 Pioneer Hi Bred Int A method of producing transgenic cereal plants
US7102056B1 (en) * 1997-04-29 2006-09-05 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration

Also Published As

Publication number Publication date
EP1876242A2 (fr) 2008-01-09
EP1261729A2 (fr) 2002-12-04
CA2401247A1 (fr) 2001-08-30
AU5505001A (en) 2001-09-03
CN1426476A (zh) 2003-06-25
WO2001062890A8 (fr) 2002-03-21
EP1876242A3 (fr) 2008-03-05
WO2001062890A3 (fr) 2001-12-27
WO2001062890A2 (fr) 2001-08-30
US20030140382A1 (en) 2003-07-24
JP2004505605A (ja) 2004-02-26

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