ZA200200333B - Novel carboxylic acid derivatives with 5,6 substituted pyrimidine ring, the production and utilization thereof as endothelin receptor antagonists. - Google Patents
Novel carboxylic acid derivatives with 5,6 substituted pyrimidine ring, the production and utilization thereof as endothelin receptor antagonists. Download PDFInfo
- Publication number
- ZA200200333B ZA200200333B ZA200200333A ZA200200333A ZA200200333B ZA 200200333 B ZA200200333 B ZA 200200333B ZA 200200333 A ZA200200333 A ZA 200200333A ZA 200200333 A ZA200200333 A ZA 200200333A ZA 200200333 B ZA200200333 B ZA 200200333B
- Authority
- ZA
- South Africa
- Prior art keywords
- alkyl
- alkoxy
- alkylthio
- phenyl
- compounds
- Prior art date
Links
- 229940118365 Endothelin receptor antagonist Drugs 0.000 title claims description 11
- 239000002308 endothelin receptor antagonist Substances 0.000 title claims description 11
- 150000001732 carboxylic acid derivatives Chemical class 0.000 title description 8
- 125000000714 pyrimidinyl group Chemical group 0.000 title 1
- -1 mercapto, amino Chemical group 0.000 claims description 61
- 150000001875 compounds Chemical class 0.000 claims description 52
- 150000003254 radicals Chemical class 0.000 claims description 35
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 27
- 229910052736 halogen Inorganic materials 0.000 claims description 26
- 150000002367 halogens Chemical class 0.000 claims description 26
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 22
- 108050009340 Endothelin Proteins 0.000 claims description 20
- 102000002045 Endothelin Human genes 0.000 claims description 20
- ZUBDGKVDJUIMQQ-UBFCDGJISA-N endothelin-1 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)NC(=O)[C@H]1NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@@H](CC=2C=CC(O)=CC=2)NC(=O)[C@H](C(C)C)NC(=O)[C@H]2CSSC[C@@H](C(N[C@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)NC(=O)[C@@H](CO)NC(=O)[C@H](N)CSSC1)C1=CNC=N1 ZUBDGKVDJUIMQQ-UBFCDGJISA-N 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 20
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims description 17
- 229910052717 sulfur Inorganic materials 0.000 claims description 17
- 125000000951 phenoxy group Chemical group [H]C1=C([H])C([H])=C(O*)C([H])=C1[H] 0.000 claims description 16
- 125000005843 halogen group Chemical group 0.000 claims description 15
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 14
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 13
- 125000004093 cyano group Chemical group *C#N 0.000 claims description 13
- 239000011593 sulfur Substances 0.000 claims description 13
- 229910052799 carbon Inorganic materials 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 125000001624 naphthyl group Chemical group 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 239000004480 active ingredient Substances 0.000 claims description 7
- 229910052783 alkali metal Inorganic materials 0.000 claims description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 7
- 239000003112 inhibitor Substances 0.000 claims description 7
- 125000001072 heteroaryl group Chemical group 0.000 claims description 6
- 239000001257 hydrogen Substances 0.000 claims description 6
- 229910052739 hydrogen Inorganic materials 0.000 claims description 6
- 125000004433 nitrogen atom Chemical group N* 0.000 claims description 6
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 5
- 206010020772 Hypertension Diseases 0.000 claims description 5
- 102000004270 Peptidyl-Dipeptidase A Human genes 0.000 claims description 5
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 claims description 5
- 150000001340 alkali metals Chemical class 0.000 claims description 5
- 229910052784 alkaline earth metal Inorganic materials 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 4
- 206010033645 Pancreatitis Diseases 0.000 claims description 4
- CIUQDSCDWFSTQR-UHFFFAOYSA-N [C]1=CC=CC=C1 Chemical compound [C]1=CC=CC=C1 CIUQDSCDWFSTQR-UHFFFAOYSA-N 0.000 claims description 4
- 230000001154 acute effect Effects 0.000 claims description 4
- 150000001342 alkaline earth metals Chemical class 0.000 claims description 4
- 125000004450 alkenylene group Chemical group 0.000 claims description 4
- 125000002947 alkylene group Chemical group 0.000 claims description 4
- IYABWNGZIDDRAK-UHFFFAOYSA-N allene Chemical group C=C=C IYABWNGZIDDRAK-UHFFFAOYSA-N 0.000 claims description 4
- 150000005840 aryl radicals Chemical class 0.000 claims description 4
- 208000006673 asthma Diseases 0.000 claims description 4
- 150000001768 cations Chemical class 0.000 claims description 4
- 125000005678 ethenylene group Chemical group [H]C([*:1])=C([H])[*:2] 0.000 claims description 4
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 claims description 4
- 125000004430 oxygen atom Chemical group O* 0.000 claims description 4
- 208000002815 pulmonary hypertension Diseases 0.000 claims description 4
- 125000004434 sulfur atom Chemical group 0.000 claims description 4
- 208000009304 Acute Kidney Injury Diseases 0.000 claims description 3
- 206010004446 Benign prostatic hyperplasia Diseases 0.000 claims description 3
- 201000006474 Brain Ischemia Diseases 0.000 claims description 3
- 206010007558 Cardiac failure chronic Diseases 0.000 claims description 3
- 206010008120 Cerebral ischaemia Diseases 0.000 claims description 3
- 206010033647 Pancreatitis acute Diseases 0.000 claims description 3
- 206010060862 Prostate cancer Diseases 0.000 claims description 3
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 3
- 208000033626 Renal failure acute Diseases 0.000 claims description 3
- 201000011040 acute kidney failure Diseases 0.000 claims description 3
- 201000003229 acute pancreatitis Diseases 0.000 claims description 3
- 208000012998 acute renal failure Diseases 0.000 claims description 3
- 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 claims description 3
- 206010008118 cerebral infarction Diseases 0.000 claims description 3
- 208000020832 chronic kidney disease Diseases 0.000 claims description 3
- 208000022831 chronic renal failure syndrome Diseases 0.000 claims description 3
- 150000002431 hydrogen Chemical class 0.000 claims description 3
- 230000036454 renin-angiotensin system Effects 0.000 claims description 3
- 208000037803 restenosis Diseases 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 125000003831 tetrazolyl group Chemical group 0.000 claims description 3
- 239000002934 diuretic Substances 0.000 claims description 2
- 229940030606 diuretics Drugs 0.000 claims description 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 2
- 125000003226 pyrazolyl group Chemical group 0.000 claims description 2
- 125000000168 pyrrolyl group Chemical group 0.000 claims description 2
- 239000002461 renin inhibitor Substances 0.000 claims description 2
- 229940086526 renin-inhibitors Drugs 0.000 claims description 2
- 125000001425 triazolyl group Chemical group 0.000 claims description 2
- 239000000825 pharmaceutical preparation Substances 0.000 claims 6
- 201000010099 disease Diseases 0.000 claims 5
- 102000003729 Neprilysin Human genes 0.000 claims 2
- 108090000028 Neprilysin Proteins 0.000 claims 2
- PXQLVRUNWNTZOS-UHFFFAOYSA-N sulfanyl Chemical class [SH] PXQLVRUNWNTZOS-UHFFFAOYSA-N 0.000 claims 2
- 125000004178 (C1-C4) alkyl group Chemical group 0.000 claims 1
- 229940123413 Angiotensin II antagonist Drugs 0.000 claims 1
- 229940127291 Calcium channel antagonist Drugs 0.000 claims 1
- 239000002333 angiotensin II receptor antagonist Substances 0.000 claims 1
- 239000000480 calcium channel blocker Substances 0.000 claims 1
- 125000002883 imidazolyl group Chemical group 0.000 claims 1
- 229940124531 pharmaceutical excipient Drugs 0.000 claims 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 52
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 27
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 15
- 239000000243 solution Substances 0.000 description 14
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 12
- 239000002904 solvent Substances 0.000 description 12
- 102000005962 receptors Human genes 0.000 description 11
- 108020003175 receptors Proteins 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 239000002585 base Substances 0.000 description 9
- 239000012071 phase Substances 0.000 description 8
- 238000005160 1H NMR spectroscopy Methods 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 239000000741 silica gel Substances 0.000 description 6
- 229910002027 silica gel Inorganic materials 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 125000001424 substituent group Chemical group 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 5
- 230000036772 blood pressure Effects 0.000 description 5
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000012074 organic phase Substances 0.000 description 5
- 229910000104 sodium hydride Inorganic materials 0.000 description 5
- 229910052938 sodium sulfate Inorganic materials 0.000 description 5
- 235000011152 sodium sulphate Nutrition 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 101800004490 Endothelin-1 Proteins 0.000 description 4
- 102100033902 Endothelin-1 Human genes 0.000 description 4
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 4
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 4
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 4
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 4
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 4
- 239000008186 active pharmaceutical agent Substances 0.000 description 4
- 239000005557 antagonist Substances 0.000 description 4
- 239000005457 ice water Substances 0.000 description 4
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 4
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 4
- 239000012312 sodium hydride Substances 0.000 description 4
- FXTPUVLWVRTMHV-UHFFFAOYSA-N 2-(4-methoxy-5-methylpyrimidin-2-yl)oxy-3,3-diphenyl-3-phenylmethoxypropanoic acid Chemical compound C1=C(C)C(OC)=NC(OC(C(O)=O)C(OCC=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=N1 FXTPUVLWVRTMHV-UHFFFAOYSA-N 0.000 description 3
- RCJNDDNXUYCUOY-UHFFFAOYSA-N 2-chloro-4-methoxy-5-methylpyrimidine Chemical compound COC1=NC(Cl)=NC=C1C RCJNDDNXUYCUOY-UHFFFAOYSA-N 0.000 description 3
- IKVBTRHAPPNLNI-UHFFFAOYSA-N 4-methoxy-5-methyl-2-methylsulfanylpyrimidine Chemical compound COC1=NC(SC)=NC=C1C IKVBTRHAPPNLNI-UHFFFAOYSA-N 0.000 description 3
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 208000001647 Renal Insufficiency Diseases 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 150000001735 carboxylic acids Chemical class 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 239000000543 intermediate Substances 0.000 description 3
- 201000006370 kidney failure Diseases 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 235000019341 magnesium sulphate Nutrition 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- MFDUCCDTBLZMSZ-UHFFFAOYSA-N 2-(4-methoxy-5-methylpyrimidin-2-yl)oxy-3,3-diphenyl-3-propan-2-yloxypropanoic acid Chemical compound C1=C(C)C(OC)=NC(OC(C(O)=O)C(OC(C)C)(C=2C=CC=CC=2)C=2C=CC=CC=2)=N1 MFDUCCDTBLZMSZ-UHFFFAOYSA-N 0.000 description 2
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical class NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- IFQZZMKLFMUHRW-UHFFFAOYSA-N 2-methylsulfanyl-6,7-dihydro-5h-cyclopenta[d]pyrimidine Chemical compound CSC1=NC=C2CCCC2=N1 IFQZZMKLFMUHRW-UHFFFAOYSA-N 0.000 description 2
- LPSCEUCZQDWXAF-UHFFFAOYSA-N 2-methylsulfonyl-6,7-dihydro-5h-cyclopenta[d]pyrimidine Chemical compound CS(=O)(=O)C1=NC=C2CCCC2=N1 LPSCEUCZQDWXAF-UHFFFAOYSA-N 0.000 description 2
- QGPPVJOASIAFMG-UHFFFAOYSA-N 3-ethoxy-2-(4-methoxy-5-methylpyrimidin-2-yl)oxy-3,3-diphenylpropanoic acid Chemical compound C=1C=CC=CC=1C(C=1C=CC=CC=1)(OCC)C(C(O)=O)OC1=NC=C(C)C(OC)=N1 QGPPVJOASIAFMG-UHFFFAOYSA-N 0.000 description 2
- NNRJLMFGHYARTK-UHFFFAOYSA-N 3-hydroxy-2-(4-methoxy-5-methylpyrimidin-2-yl)oxy-3,3-diphenylpropanoic acid Chemical compound COc1nc(OC(C(O)=O)C(O)(c2ccccc2)c2ccccc2)ncc1C NNRJLMFGHYARTK-UHFFFAOYSA-N 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 2
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000010180 Endothelin receptor Human genes 0.000 description 2
- 108050001739 Endothelin receptor Proteins 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 description 2
- MZRVEZGGRBJDDB-UHFFFAOYSA-N N-Butyllithium Chemical compound [Li]CCCC MZRVEZGGRBJDDB-UHFFFAOYSA-N 0.000 description 2
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical class C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- WQDUMFSSJAZKTM-UHFFFAOYSA-N Sodium methoxide Chemical compound [Na+].[O-]C WQDUMFSSJAZKTM-UHFFFAOYSA-N 0.000 description 2
- 208000006011 Stroke Diseases 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 206010047139 Vasoconstriction Diseases 0.000 description 2
- 125000004453 alkoxycarbonyl group Chemical group 0.000 description 2
- VIROVYVQCGLCII-UHFFFAOYSA-N amobarbital Chemical compound CC(C)CCC1(CC)C(=O)NC(=O)NC1=O VIROVYVQCGLCII-UHFFFAOYSA-N 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- DUMGMNCUUJROAO-UHFFFAOYSA-N benzyl 2-(6,7-dihydro-5h-cyclopenta[d]pyrimidin-2-yloxy)-3-methoxy-3,3-diphenylpropanoate Chemical compound C=1C=CC=CC=1C(C=1C=CC=CC=1)(OC)C(OC=1N=C2CCCC2=CN=1)C(=O)OCC1=CC=CC=C1 DUMGMNCUUJROAO-UHFFFAOYSA-N 0.000 description 2
- HZZGDPLAJHVHSP-GKHTVLBPSA-N big endothelin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(O)=O)NC(=O)[C@H]1NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@H](C(C)C)NC(=O)[C@@H]2CSSC[C@@H](C(N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CSSC1)C1=CN=CN1 HZZGDPLAJHVHSP-GKHTVLBPSA-N 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 2
- 229910052794 bromium Inorganic materials 0.000 description 2
- 229910052792 caesium Inorganic materials 0.000 description 2
- 210000001715 carotid artery Anatomy 0.000 description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 230000008602 contraction Effects 0.000 description 2
- 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 239000012442 inert solvent Substances 0.000 description 2
- 208000001286 intracranial vasospasm Diseases 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 210000004731 jugular vein Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- ZCSHNCUQKCANBX-UHFFFAOYSA-N lithium diisopropylamide Chemical compound [Li+].CC(C)[N-]C(C)C ZCSHNCUQKCANBX-UHFFFAOYSA-N 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 208000010125 myocardial infarction Diseases 0.000 description 2
- 229910052763 palladium Inorganic materials 0.000 description 2
- 229910000027 potassium carbonate Inorganic materials 0.000 description 2
- 229940002612 prodrug Drugs 0.000 description 2
- 239000000651 prodrug Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 150000003230 pyrimidines Chemical class 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- 125000003396 thiol group Chemical class [H]S* 0.000 description 2
- 125000000876 trifluoromethoxy group Chemical group FC(F)(F)O* 0.000 description 2
- 230000025033 vasoconstriction Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- RQJWOLFMWKZKCJ-CQSZACIVSA-N (2s)-2-hydroxy-3-methoxy-3,3-diphenylpropanoic acid Chemical compound C=1C=CC=CC=1C([C@H](O)C(O)=O)(OC)C1=CC=CC=C1 RQJWOLFMWKZKCJ-CQSZACIVSA-N 0.000 description 1
- 125000004765 (C1-C4) haloalkyl group Chemical group 0.000 description 1
- FIARMZDBEGVMLV-UHFFFAOYSA-N 1,1,2,2,2-pentafluoroethanolate Chemical group [O-]C(F)(F)C(F)(F)F FIARMZDBEGVMLV-UHFFFAOYSA-N 0.000 description 1
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- 125000004973 1-butenyl group Chemical group C(=CCC)* 0.000 description 1
- DURPTKYDGMDSBL-UHFFFAOYSA-N 1-butoxybutane Chemical compound CCCCOCCCC DURPTKYDGMDSBL-UHFFFAOYSA-N 0.000 description 1
- 125000004776 1-fluoroethyl group Chemical group [H]C([H])([H])C([H])(F)* 0.000 description 1
- 125000000453 2,2,2-trichloroethyl group Chemical group [H]C([H])(*)C(Cl)(Cl)Cl 0.000 description 1
- 125000004206 2,2,2-trifluoroethyl group Chemical group [H]C([H])(*)C(F)(F)F 0.000 description 1
- 125000004781 2,2-dichloro-2-fluoroethyl group Chemical group [H]C([H])(*)C(F)(Cl)Cl 0.000 description 1
- 125000004778 2,2-difluoroethyl group Chemical group [H]C([H])(*)C([H])(F)F 0.000 description 1
- ZFFMLCVRJBZUDZ-UHFFFAOYSA-N 2,3-dimethylbutane Chemical group CC(C)C(C)C ZFFMLCVRJBZUDZ-UHFFFAOYSA-N 0.000 description 1
- DQXNTSXKIUZJJS-UHFFFAOYSA-N 2,4-dichloro-5-methylpyrimidine Chemical compound CC1=CN=C(Cl)N=C1Cl DQXNTSXKIUZJJS-UHFFFAOYSA-N 0.000 description 1
- SRXFXCKTIGELTI-UHFFFAOYSA-N 2-(4-chlorophenyl)ethanamine Chemical compound NCCC1=CC=C(Cl)C=C1 SRXFXCKTIGELTI-UHFFFAOYSA-N 0.000 description 1
- JDOXCEWQNADMCF-UHFFFAOYSA-N 2-(6,7-dihydro-5H-cyclopenta[d]pyrimidin-2-yloxy)-3-methoxy-3,3-diphenylpropanoic acid Chemical compound COC(C(Oc1ncc2CCCc2n1)C(O)=O)(c1ccccc1)c1ccccc1 JDOXCEWQNADMCF-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 125000004974 2-butenyl group Chemical group C(C=CC)* 0.000 description 1
- 125000004780 2-chloro-2,2-difluoroethyl group Chemical group [H]C([H])(*)C(F)(F)Cl 0.000 description 1
- 125000004791 2-fluoroethoxy group Chemical group FCCO* 0.000 description 1
- 125000004777 2-fluoroethyl group Chemical group [H]C([H])(F)C([H])([H])* 0.000 description 1
- UGTOJOHCAPCWPX-UHFFFAOYSA-N 2-hydroxy-3,3-diphenyl-3-phenylmethoxypropanoic acid Chemical compound C=1C=CC=CC=1C(C=1C=CC=CC=1)(C(C(O)=O)O)OCC1=CC=CC=C1 UGTOJOHCAPCWPX-UHFFFAOYSA-N 0.000 description 1
- LXYDMYXWEUDFIC-UHFFFAOYSA-N 2-hydroxy-3,3-diphenyl-3-propan-2-yloxypropanoic acid Chemical compound C=1C=CC=CC=1C(C(O)C(O)=O)(OC(C)C)C1=CC=CC=C1 LXYDMYXWEUDFIC-UHFFFAOYSA-N 0.000 description 1
- ZAIBWUAGAZIQMM-UHFFFAOYSA-N 2-oxocyclopentane-1-carbaldehyde Chemical compound O=CC1CCCC1=O ZAIBWUAGAZIQMM-UHFFFAOYSA-N 0.000 description 1
- WADSJYLPJPTMLN-UHFFFAOYSA-N 3-(cycloundecen-1-yl)-1,2-diazacycloundec-2-ene Chemical compound C1CCCCCCCCC=C1C1=NNCCCCCCCC1 WADSJYLPJPTMLN-UHFFFAOYSA-N 0.000 description 1
- DSPISRPQOMBZAW-UHFFFAOYSA-N 3-[2-(3,4-dimethoxyphenyl)ethoxy]-2-(4-methoxy-5-methylpyrimidin-2-yl)oxy-3,3-diphenylpropanoic acid Chemical compound COC=1C=C(C=CC=1OC)CCOC(C(C(=O)O)OC1=NC=C(C(=N1)OC)C)(C1=CC=CC=C1)C1=CC=CC=C1 DSPISRPQOMBZAW-UHFFFAOYSA-N 0.000 description 1
- CSDQQAQKBAQLLE-UHFFFAOYSA-N 4-(4-chlorophenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridine Chemical compound C1=CC(Cl)=CC=C1C1C(C=CS2)=C2CCN1 CSDQQAQKBAQLLE-UHFFFAOYSA-N 0.000 description 1
- 125000004203 4-hydroxyphenyl group Chemical group [H]OC1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- FJFJLOMVXWGBDU-UHFFFAOYSA-N 4-methoxy-5-methyl-2-methylsulfonylpyrimidine Chemical compound COC1=NC(S(C)(=O)=O)=NC=C1C FJFJLOMVXWGBDU-UHFFFAOYSA-N 0.000 description 1
- 239000005541 ACE inhibitor Substances 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 108010064733 Angiotensins Proteins 0.000 description 1
- 102000015427 Angiotensins Human genes 0.000 description 1
- 108010001478 Bacitracin Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102400000686 Endothelin-1 Human genes 0.000 description 1
- 102100029110 Endothelin-2 Human genes 0.000 description 1
- 108090000387 Endothelin-2 Proteins 0.000 description 1
- 206010014824 Endotoxic shock Diseases 0.000 description 1
- 208000010228 Erectile Dysfunction Diseases 0.000 description 1
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical class NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 206010061459 Gastrointestinal ulcer Diseases 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 208000019695 Migraine disease Diseases 0.000 description 1
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 description 1
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 206010053159 Organ failure Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
- 208000003782 Raynaud disease Diseases 0.000 description 1
- 208000012322 Raynaud phenomenon Diseases 0.000 description 1
- 206010040070 Septic Shock Diseases 0.000 description 1
- 208000032851 Subarachnoid Hemorrhage Diseases 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 235000005505 Ziziphus oenoplia Nutrition 0.000 description 1
- 244000104547 Ziziphus oenoplia Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 125000002723 alicyclic group Chemical group 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 229910000288 alkali metal carbonate Inorganic materials 0.000 description 1
- 150000008041 alkali metal carbonates Chemical class 0.000 description 1
- 125000004414 alkyl thio group Chemical group 0.000 description 1
- 125000005336 allyloxy group Chemical group 0.000 description 1
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229960001301 amobarbital Drugs 0.000 description 1
- 238000002399 angioplasty Methods 0.000 description 1
- 229940044094 angiotensin-converting-enzyme inhibitor Drugs 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- 229960003071 bacitracin Drugs 0.000 description 1
- 229930184125 bacitracin Natural products 0.000 description 1
- CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- LPYKDGZLQIPQQP-UHFFFAOYSA-N benzyl 2-hydroxy-3-methoxy-3,3-diphenylpropanoate Chemical compound C=1C=CC=CC=1C(C=1C=CC=CC=1)(OC)C(O)C(=O)OCC1=CC=CC=C1 LPYKDGZLQIPQQP-UHFFFAOYSA-N 0.000 description 1
- 239000002876 beta blocker Substances 0.000 description 1
- 229940097320 beta blocking agent Drugs 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000004067 bulking agent Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 125000003917 carbamoyl group Chemical group [H]N([H])C(*)=O 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 150000003857 carboxamides Chemical class 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- HRYZWHHZPQKTII-UHFFFAOYSA-N chloroethane Chemical compound CCCl HRYZWHHZPQKTII-UHFFFAOYSA-N 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 125000001995 cyclobutyl group Chemical group [H]C1([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
- 125000000582 cycloheptyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
- 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
- 125000001559 cyclopropyl group Chemical group [H]C1([H])C([H])([H])C1([H])* 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000012024 dehydrating agents Substances 0.000 description 1
- MHDVGSVTJDSBDK-UHFFFAOYSA-N dibenzyl ether Chemical compound C=1C=CC=CC=1COCC1=CC=CC=C1 MHDVGSVTJDSBDK-UHFFFAOYSA-N 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical class OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical class CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 210000003989 endothelium vascular Anatomy 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000004185 ester group Chemical group 0.000 description 1
- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 description 1
- 125000003754 ethoxycarbonyl group Chemical group C(=O)(OCC)* 0.000 description 1
- 229960003750 ethyl chloride Drugs 0.000 description 1
- 125000004672 ethylcarbonyl group Chemical group [H]C([H])([H])C([H])([H])C(*)=O 0.000 description 1
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 125000004216 fluoromethyl group Chemical group [H]C([H])(F)* 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 150000004820 halides Chemical class 0.000 description 1
- 125000003187 heptyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- ORTFAQDWJHRMNX-UHFFFAOYSA-N hydroxidooxidocarbon(.) Chemical group O[C]=O ORTFAQDWJHRMNX-UHFFFAOYSA-N 0.000 description 1
- JBFYUZGYRGXSFL-UHFFFAOYSA-N imidazolide Chemical compound C1=C[N-]C=N1 JBFYUZGYRGXSFL-UHFFFAOYSA-N 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003701 inert diluent Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000035987 intoxication Effects 0.000 description 1
- 231100000566 intoxication Toxicity 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000302 ischemic effect Effects 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 201000008806 mesenchymal cell neoplasm Diseases 0.000 description 1
- 229910000000 metal hydroxide Inorganic materials 0.000 description 1
- 150000004692 metal hydroxides Chemical class 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 125000001160 methoxycarbonyl group Chemical group [H]C([H])([H])OC(*)=O 0.000 description 1
- NNBBQNFHCVVQHZ-UHFFFAOYSA-N methyl carbamimidothioate;sulfuric acid Chemical compound CSC(N)=N.OS(O)(=O)=O NNBBQNFHCVVQHZ-UHFFFAOYSA-N 0.000 description 1
- 125000004170 methylsulfonyl group Chemical group [H]C([H])([H])S(*)(=O)=O 0.000 description 1
- 206010027599 migraine Diseases 0.000 description 1
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 210000000885 nephron Anatomy 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 150000002902 organometallic compounds Chemical class 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 125000006340 pentafluoro ethyl group Chemical group FC(F)(F)C(F)(F)* 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- NTTOTNSKUYCDAV-UHFFFAOYSA-N potassium hydride Chemical compound [KH] NTTOTNSKUYCDAV-UHFFFAOYSA-N 0.000 description 1
- 229910000105 potassium hydride Inorganic materials 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- FVSKHRXBFJPNKK-UHFFFAOYSA-N propionitrile Chemical compound CCC#N FVSKHRXBFJPNKK-UHFFFAOYSA-N 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 239000002287 radioligand Substances 0.000 description 1
- 229940044551 receptor antagonist Drugs 0.000 description 1
- 239000002464 receptor antagonist Substances 0.000 description 1
- 238000001525 receptor binding assay Methods 0.000 description 1
- 206010038464 renal hypertension Diseases 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000012453 sprague-dawley rat model Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- HXJUTPCZVOIRIF-UHFFFAOYSA-N sulfolane Chemical compound O=S1(=O)CCCC1 HXJUTPCZVOIRIF-UHFFFAOYSA-N 0.000 description 1
- 150000003457 sulfones Chemical class 0.000 description 1
- 150000003462 sulfoxides Chemical class 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000007885 tablet disintegrant Substances 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 125000005147 toluenesulfonyl group Chemical group C=1(C(=CC=CC1)S(=O)(=O)*)C 0.000 description 1
- 238000005690 transetherification reaction Methods 0.000 description 1
- CMMXCVYESRODNH-UHFFFAOYSA-N trichloroepoxyethane Chemical class ClC1OC1(Cl)Cl CMMXCVYESRODNH-UHFFFAOYSA-N 0.000 description 1
- 125000002023 trifluoromethyl group Chemical group FC(F)(F)* 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000002525 vasculotropin inhibitor Substances 0.000 description 1
- 239000005526 vasoconstrictor agent Substances 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D473/00—Heterocyclic compounds containing purine ring systems
- C07D473/26—Heterocyclic compounds containing purine ring systems with an oxygen, sulphur, or nitrogen atom directly attached in position 2 or 6, but not in both
- C07D473/28—Oxygen atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/14—Drugs for disorders of the endocrine system of the thyroid hormones, e.g. T3, T4
- A61P5/16—Drugs for disorders of the endocrine system of the thyroid hormones, e.g. T3, T4 for decreasing, blocking or antagonising the activity of the thyroid hormones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
- C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
- C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
- C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
- C07D239/32—One oxygen, sulfur or nitrogen atom
- C07D239/34—One oxygen atom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
- C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
- C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
- C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
- C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
- C07D239/52—Two oxygen atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
- C07D239/70—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings condensed with carbocyclic rings or ring systems
Landscapes
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Urology & Nephrology (AREA)
- Pulmonology (AREA)
- Hospice & Palliative Care (AREA)
- Vascular Medicine (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Hydrogenated Pyridines (AREA)
Description
fh " x 4 o 0050/50501
Novel carboxylic acid derivatives with 5,6-substituted pyrimidine ring, their preparation and use as endothelin receptor antagonists
The present invention relates to novel carboxylic acid derivatives, their preparation and use.
Endothelin is a peptide which is composed of 21 aminoacids and is synthesized and released by vascular endothelium. Endothelin exists in three isoforms, ET-1, ET-2 and
ET-3. “Endothelin” or "ET” hereinafter refers to one or all isoforms of endothelin.
Endothelin is a potent vasoconstrictor and has a strong effect on vessel tone. It is known that this vasoconstriction is caused by binding endothelin to its receptor (Nature, 332, 411-415, 1988; FEBS Letters, 231, 440-444, 1988 and Biochem. Biophys. Res.
Commun., 154, 868-875, 1988).
Elevated or abnormal release of endothelin causes persistent vasoconstriction in peripheral, renal and cerebral blood vessels, which may result in disorders. As reported in the literature, endothelin is involved in a number of disorders. These include: hypertension, acute myocardial infarct, pulmonary hypertension, Raynaud's syndrome, cerebral vasospasms, stroke, benign prostate hypertrophy, atherosclerosis, asthma and prostate cancer (J. Vascular Med. Biology 2, 207 (1990), J. Am. Med. Association 264, 2868 (1990), Nature 344, 114 (1990), N. Engl. J. Med. 322, 205 (1989), N. Engl. J. Med. 328, 1732 (1993), Nephron 66, 373 (1994), Stroke 25, 904 (1994), Nature 365, 759 (1993), J. Mol. Cell. Cardiol. 27, A234 (1995); Cancer Research 56, 663 (1996), Nature
Medicine 1, 944,(1995)).
At least 2 endothelin receptor subtypes, ET, and ETg receptors, are currently described in the literature (Nature 348, 730 (1990), Nature 348, 732 (1990)). Accordingly, substances which inhibit the binding of endothelin to one or both receptors ought to antagonize the physiological effects of endothelin and therefore represent valuable drugs.
The preparation and use of endothelin receptor antagonists has already been described in WO 95/26716, WO 96/11914, WO 97/09294, WO097/12878, WO 97/38980,
WO097/38981, WO 97/38982, WO098/09953, WO098/27070, DE 19726146.9, DE 19748238.4, DE 19750529.5, DE 19806438.1, DE 19809144.3 and DE 19836044.4.
Further investigation has revealed that related compounds with 5,6-substituted pyrimidine ring has advantageous properties in relation to receptor affinity and receptor i]
AMENDED SHEET
® 0050/50501 -2.- binding profile. The present patent relates to their preparation and use.
The invention relates to carboxylic acid derivatives of the formula
R® a [ i: | N
R—z—c—C—o— Dg
KE 2 B= ; big
R in which R is tetrazolyl or a group
Cc . 1].
C—R in which R has the following meaning: : “ay anOR'radicatinwhichR'isr hydrogen, the cation of an alkali metal, the cation of an alkaline earth metal, a physiologically tolerated organic ammonium ion such as tertiary C4-C,- alkylammonium or the ammonium ion;
Cs-Cg-cycloalkyl, Cy-Cg-alkyl, CHp-phenyl which may be substituted by one or more of the following radicals: halogen, nitro, cyano, C,-Cy-alkyl, C4-C4-haloalkyl, hydroxyl, C4-C,-alkoxy, mercapto, C;-C,-alkylthio, amino,
NH(C;-Cy-alkyl), N(C4-Cs-alkyl),; a C;-Ce-alkenyl or a C;-Ce-alkynyl group, it being possible for these groups in turn to carry from one to five halogen atoms;
R’ can also be a phenyl radical which can carry from one to five halogen atoms and/or from one to three of the following radicals: nitro, cyano, C4-C4-alkyl, Cy-C,- haloalkyl, hydroxyl, C-Cs-alkoxy, mercapto, C;-C,-alkylthio, amino,
NH(C,-C,-alkyl), N(C+-Cs-alkyl)z;
SI
~ ‘0050/50501 -3- b) a 5-membered heteroaromatic system, such as pyrrolyl, pyrazolyl, imidazoly! and triazolyl, which is linked via a nitrogen atom and which may carry from one to two halogen atoms or from one to two C4-C,-alkyl or from one to two C;-Cs-alkoxy groups;
C) a group (Bs —0— (CB,),—S—R in which k has the values 0, 1 and 2, p has the values 1, 2, 3 and 4, and R® is
C4-Cy-alkyl, Cs-Cg-cycloalkyl, Cs-Cs-alkenyl, C3-Cg-alkynyl or phenyl which may be substituted by one or more, e.g. from one to three, of the following radicals: halogen, nitro, cyano, C4-Cy-alkyl, C,-Cs-haloalkyl, hydroxyl, C,-C4-alkoxy, C4-C4- i5 alkylthio, mercapto, amino, NH(C;-Cy-alkyl), N(C-Cg-alkyl),; d) a radical oO —¥—8— ®
I
© in which R? is:
C1-Cs-alkyl, C3-Cs-alkenyl, Cs-Cs-alkynyl, C3-Cg-cycloalkyl, it being possible for these radicals to carry a C4-C4-alkoxy, C4-C,-alkylthio and/or a phenyl radical as mentioned under C); phenyl which may be substituted by from one to three of the following radicals: halogen, nitro, cyano, C4-Cs-alkyl, C4-C4-haloalkyl, hydroxyl, C,-Cs-alkoxy, C4-C,- alkylthio, mercapto, amino, NH(C;-Cy4-alkyl), N(C4-Cs-alkyl)..
The other substituents have the following meanings:
LS A
~ '0050/50501 -4-
R? is hydroxyl, NH,, NH(C1-Cs-alkyl), N(C1-Cs-alkyl),, C-Cy-alkyl, C>-Cs-alkenyl, Co-
Cs-alkynyl, C4-C4-hydroxyalkyl, C;-C,-haloalkyl, C,-Cs-alkoxy, C;-Cs-haloalkoxy,
C1-Cs-alkylthio or CR? forms together with CR® a 5- or 6- membered alkylene or alkenylene ring which may be substituted by one or two C,-Cy-alkyl groups, in which in each case one or more methylene groups may be replaced by oxygen, sulfur, -NH or —N(C;-C,-alkyl).
R® is hydroxyl, NHz, NH(C:-Cy4-alkyl), N(C4-Cy4-alkyl),, halogen, C4-Cs-alkyl, C>-Cs- alkenyl, Co-Cy-alkynyl, Cs-Ce-alkenyloxy, C,-Cs-alkylcarbonyl, C4-Cqs- alkoxycarbonyl, C;-Cs-hydroxyalkyl, C4-Cs-haloalkyl, C,-C4-alkoxy, C;-C4- haloalkoxy, -NH-O-C;-C4-alkyl, C-C,-alkylthio or CR® forms, as indicated under
R?, together with CR? a 5- or 6-membered ring;
R* and R® (which may be identical or different) are: phenyl or naphthyl, each of which may be substituted by one or more of the following radicals: halogen, nitro, cyano, hydroxyl, C4-C4-alkyl, C4-C4-haloalkyl,
C,-Cs-alkoxy, Cq-Cs-haloalkoxy, phenoxy, C;-Cs-alkylthio, amino, NH(C;-C,- alkyl), N(C4-Cs-alkyl),; or phenyl or naphthyl which are connected together in ortho positions by a direct linkage, a methylene, ethylene or ethenylene group, an oxygen or sulfur atom or an SO., NH or N-alkyl group; or C3-C-cycloalkyl;
R® is hydrogen,
C,-Cg-alkyl, C3-Ce-alkenyl, Cs-Cg-alkynyl or C5-Cg-cycloalkyl, it being possible for each of these radicals to be substituted one or more times by: hydroxyl, mercapto, carboxyl, halogen, nitro, cyano, C,-Cs-alkoxy, Cs-Cs-alkenyloxy, Cs-Cs- alkynyloxy, C;-Cs-alkylthio, C;-Cs-haloalkoxy, C;-Cs-alkylcarbonyl, C;-C,- alkoxycarbonyl, (C4-Cs-alkyl)NHcarbonyl, (C;-Cs-alkyl)o.Ncarbonyl, Cs-C,- alkylcarbonylalkyl, amino, NH(C-Cy-alkyl), N(C4-Cs-alkyl),, phenoxy or phenyl, it being possible for said aryl radicals to be substituted one or more times, e.g. from one to three times, by halogen, nitro, cyano, Cy-C4-alkyl, C,-C,-haloalkyl, C4-C,-
AMENDED SHEET
® 0050/50501 -5- alkoxy, C;-Cs-haloalkoxy, mercapto, carboxy, hydroxyl, amino, R', C,-Cs alkoxycarbonyl, NH(C,-Cg4-alkyl), N(C4-C4-alkyl),, methylenedioxy, ethylenedioxy, or phenyl or phenoxy substituted by C;-Cs-alkylthio; phenyl or naphthyl, each of which may be substituted by one or more of the following radicals: halogen, nitro, cyano, hydroxyl, amino, C;-C4-alkyl, C4-Cs- haloalkyl, C;-Cs-alkoxy, Cy-Cs-haloalkoxy, phenoxy, Ci-Cs-alkylthio, NH(C4-Ca- alkyl), N(C4-C,-alkyl), or methylenedioxy or ethylenedioxy; a five- or six-membered heteroaromatic system which contains from one to three nitrogen atoms and/or a sulfur or oxygen atom and which may carry from one to four halogen atoms and/or from one to two of the following radicals: C4-Cg-alkyi,
C,-Cs-haloalkyl, C4-C4-alkoxy, C,-C,-haloatkoxy, C4-Cs-alkylthio, phenyl, phenoxy or phenylcarbonyl, it being possible for the phenyl radicals is turn to carry from one to five halogen atoms and/or from one to three of the following radicals: C;-
Cs-alkyl, C1-C4-haloalkyl, C;-C4-alkoxy, C4-Cs-haloalkoxy and/or C4-Cs-alkyithio;
R'™ is Cy-Cs-alkyl, Ci-Ca-alkylthio or Ci-Cs-alkoxy, each of which carry one of the following radicals: hydroxyl, carboxyl, amino, NH(C;-Cs-alkyl), N(C;-Cs-alkyl), carboxamide or CON(C-C4-alkyl)z; : y4 is sulfur or oxygen. : SEE
The following definitions apply herein and hereinafter: an alkali metal is, for example, lithium, sodium, potassium; Cn : an alkaline earth metal is, for example, calcium, magnesium, barium; : organic ammonium jons are protonated amines such as, for example, ethanolamine, diethanolamine, ethylenediamine, diethylamine or piperazine;
C,-Cr-cycloalkyl is, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl;
C,-Cs-haloalkyl can be linear or branched such as, for example, fluoromethyl, difluoromethyt, trifluoromethyl, - chlorodifluoromethyl, dichlorofiuoromethyl,
i
AMENDED SHEET
0050/50501 -6- trichloromethyl, 1-fluoroethyl, 2-fluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, 2- chloro-2,2-difluoroethyl, 2,2-dichloro-2-fluoroethyl, 2,2,2-trichloroethyl or pentafluoroethyl;
C4-Cs-haloalkoxy can be linear or branched such as, for example, difluoromethoxy, trifluoromethoxy, chlorodifluoromethoxy, 1-fluoroethoxy, 2,2-difluoroethoxy, 1,1,2,2- tetrafluoroethoxy, 2,2,2-trifluoroethoxy, 2-chloro-1,1,2-trifluoroethoxy, .2-fluoroethoxy or pentafluoroethoxy;
C,-Cs-alkyl can be linear or branched such as, for example, methyl, ethyl, 1-propyl, 2- propyl, 2-methyi-2-propyi, 2-methyi-i-propyi, 1-butyl or 2-butyl;
C,-Cs-alkenyl can be linear or branched such as, for example, ethenyl, i-propen-3-yl, i- propen-2-yl, 1-propen-1-yl, 2-methyl-i-propenyl, 1-butenyl or 2-butenyl;
C,-Cs-alkynyl can be linear or branched such as, for example, ethynyl, i-propyn-i-yl, i- propyn-3-yl, 1-butyn-4-yl or 2-butyn-4-yl; :
C:-C4-alkoxy can be linear or branched such as, for example, methoxy, ethoxy, propoxy, : 1-methylethoxy, butoxy, 1-methylpropoxy, 2-methylpropoxy or 1,1-dimethylethoxy;
Ci-Cs-alkenyloxy can be linear or branched such as, for example, allyloxy, 2-buten-1i- yloxy or 3-buten-2-yloxy,
Cs;-Cs-alkynyloxy can be linear or branched such as, for example, 2-propyn-1-yloxy, 2- butyn-1-yloxy or 3-butyn-2-yloxy,
C1-Cy-alkylthio can be linear or branched such as, for example, methylthio, ethylthio, propylthio, 1-methylethylthio, butylthio, 1-methyipropyithio, 2-methyipropylthio or 1,1i- dimethylethylthio,
C:-Cs-alkylcarbonyl can be linear or branched such as, for example, acetyl, ethylcarbonyl or 2-propyicarbonyl;
C4-Cs-alkoxycarbonyl can be linear or branched such as, for example, methoxycarbonyl, ethoxycarbonyl, n-propoxycarbonyl, i-propoxycarbonyl or n-butoxycarbonyi;
' 0050/5050 -7-
Cs-Cg-alkylcarbonylalkyl can be linear or branched such as, for example, 2-oxoprop-1-Yl, 3-oxobut-1-yl or 3-oxobut-2-yl;
C1-Cs-alkyl can be linear or branched such as, for example, C;-C4-alkyl, pentyl, hexyl, 5 heptyl or octyl; halogen is, for example, fluorine, chlorine, bromine, iodine. :
The invention further relates to compounds from which the compounds of the formula can be released (called prodrugs).
Preferred prodrugs are those in which the release occurs under conditions prevailing in certain compartments of the body, for example in the stomach, intestine, blood stream, liver. L
The compounds and the intermediates for their preparation, such as, for example, Il and
IV, may have one or more asymmetric substituted carbon atoms. Compounds of this type may be in the form of pure enantiomers or pure diastereomers or a mixture thereof.
The use of an enantiomerically pure compound as active ingredient is preferred.
The invention further relates to the use of the abovementioned carboxylic acid derivatives for producing drugs, in particular for producing inhibitors of endothelin receptors.
The compounds of the general formula IV in which Z is sulfur or oxygen (IV) can be prepared as described in WO 96/11914. é
RB
Q 1
RE R 6 6 BH
SLANT Bogor > R —2Z C—o8 r® Rr r?
IZ ITX Iv
Compounds of the general formula Ill are either known or can be synthesized, for example, by reducing the corresponding carboxylic acids or their esters, or by other generally known methods. :
4 64 ~ ‘0050/50501 -8-
Compounds of the formula IV can be obtained in enantiomerically pure form by an acid- catalysed transetherification as described in WO 98/09953.
The enantiomerically pure compounds of the formula IV can also be obtained by carrying out a conventional racemate resolution with racemic or diastereomeric compounds of the formula IV, using suitable enantiomerically pure bases. Examples of suitable bases of this type are 4-chlorophenylethylamine and the bases mentioned in
WO 96/11914.
The novel compounds in which the substituents have the meanings stated for general formula | can be prepared, for example, by reacting the carboxylic acid derivatives of the general formula IV in which the substituents have the stated meaning with compounds of the general formula V.
B
2
IV + r* —( RR T
B® — . v rR’
R'" in formula V is halogen or R'>-SO,-, where R'? can be C;-Cj-alkyl, C;-C4-haloalkyl or phenyl. The reaction preferably takes place in an inert solvent or diluent with the addition of a suitable base, i.e. of a base that deprotonates the intermediate IV, at a temperature in the range from room temperature to the boiling point of the solvent.
If R' is an ester, then the compounds with R' = COOH can be prepared by acidic, basic or catalytic cleavage of the ester group.
Compounds of type | with R' = COOH may furthermore be obtained directly when the intermediate IV in which R' means COOH is deprotonated with two equivalents of a suitable base and reacted with compounds of the general formula V. Here too, the reaction takes places in an inert solvent and in a temperature range from room temperature to the boiling point of the solvent.
Examples of such solvents or diluents are aliphatic, alicyclic and aromatic hydrocarbons, each of which may optionally be chlorinated, such as, for example, hexane,
il
AMENDED SHEET
® 0050/50501 -9- cyclohexane, petroleum ether, naphtha, benzene, toluene, xylene, methylene chloride, chloroform, carbon tetrachloride, ethyl chloride and trichloroethylene, ethers such as, for example, diisopropyl! ether, dibutyl ether, methyl tert-butyl ether, propylene oxide, dioxane and tetrahydrofuran, nitriles such as, for example, acetonitrile and propionitrile, amides such as, for example, dimethylformamide, dimethylacetamide and N- methylpyrrolidone, sulfoxides and sulfones, such as, for example, dimethyl sulfoxide and sulfolane.
Compounds of the formula V are known, and some of them can be bought, or they can be prepared in a generally known manner.
The base which can be used is an alkali metal or alkaline earth metal hydride such as sodium hydride, potassium hydride or calcium hydride, a carbonate such as alkali metal carbonate, e.g. sodium carbonate or potassium carbonate, an alkali metal or alkaline . earth metal hydroxide such as sodium hydroxide or potassium hydroxide, an organometallic compound such as butyllithium or an alkali metal amide such as lithium diisopropylamide. )
Compounds of the formula | can also be prepared by starting from the corresponding carboxylic acids, i.e. compounds of the formula | in which ‘R' is COOH, and converting these in a conventional way into an activated form such as an acid halide, an anhydride or imidazolide, and then reacting the latter with an appropriate hydroxyl compound
HOR’. This reaction can be carried out in the conventional solvents and often requires : addition of a base such as, for example, triethylamine, pyridine, imidazole or diazabicycloundecene. These two steps can also be simplified, for example, by allowing the carboxylic acid to act in the presence of a dehydrating agent such as a carbodiimide on the hydroxyl compound.
It is also possible to prepare compounds of the formula | by starting from the salts of the corresponding carboxylic acids, i.e. from compounds of the formula I in which R' is a
COOM group where M can be an alkali metal cation or the equivalent of an alkaline earth metal cation. These salts can be reacted with many compounds of the formula R’-
A where A is a conventional nucleofugic leaving group, for example halogen such as chlorine, bromine, iodine or optionally halogen-, alkyl- or haloalkyl- substituted aryl- or alkyisulfonyl such as, for example, toluenesulfonyl and methylsulfonyl or another equivalent leaving group. Compounds of the formula R’-A with a reactive substituent A are known or can easily be obtained with general expert knowledge. This reaction can be
AMENDED SHEET
® 0050/50501 -10- carried out in the conventional solvents and is advantageously undertaken with the addition of a base, in which case those mentioned above are suitable.
In some cases it is necessary to apply generally known protective group techniques for preparing the novel compounds |. If, for example, R® = 4-hydroxyphenyl, the hydroxy! group can firstly be protected as benzyl ether, which is then cleaved at a suitable stage in the reaction sequence.
Compounds of the formula | in which R'is tetrazolyl can be prepared as described in
WO 96/11914.
With a view to the biological effect, preferred carboxylic acid derivatives of the general formula | are those — either as pure enantiomers or pure diastereomers or as mixture thereof — in which the substituents have the following meanings: :
R? hydroxyl, N(C:-Cs-alkyl)s, Ci-Cs-alkyl, Ci-Cs-haloalkyl, C-Cs-alkoxy, C4-Cs- haloalkoxy, Cs-Ca-alkylthio or CR? forms together with CR® a 5- or 6-membered alkylene or alkenylene ring which may be substituted by one or two C;-Cg-alkyl groups and in which in each case one or more methylene groups may be replaced by oxygen, sulfur, -NH or -N(C4-C4-alkyl);
R® hydroxyl, N(Ci-Cs-alkyl)s, C;-Cs-alkyl, Cy-Cs-haloalkyl, Ci-Cs-alkoxy, Ci-C,- haloalkoxy, C4-Cs4-alkylthio, halogen or CR?3 forms, as indicated for R?, together with CR? a 5- or 6-membered ring;
R* and R® phenyl or naphthyl, each of which may be substituted by one or more, e.g. from one to three, of the following radicals: halogen, cyano, hydroxyl, mercapto, amino, C;-Cgs-alkyl, C;-Cs-haloalkyl, C;-Cs-alkoxy, C;-Cs-haloalkoxy, C;-C,- alkylthio, NH(Cs-Cs-alkyl)z [sic], N(C4-Cs-alkyl)s, Ci-Cs-alkylcarbonyl, Ci-C,- alkoxycarbonyl; phenyl or naphthyl which are connected together in ortho positions by a direct linkage, a methylene, ethylene or ethenylene group, an oxygen or sulfur atom or an SO,, NH or N(C4-Cs-alkyl) group, or C3-Cs-cycloalkyl;
AMENDED SHEET
® 0050/50501 -11 -
R® C4-Cg-alkyl, Cs-Cs-alkenyl, C3-Cs-alkynyl or C3-Cg-cycloalkyl, it being possible for each of these radicals to be substituted one or more times by: halogen, hydroxyl, cyano, Cy-Cs-alkoxy, Cs-Ces-alkenyloxy, Cs-Ce-alkynyloxy, C,-Cs-alkylthio, C4-Cs- haloalkoxy, C-C,-alkylcarbonyl, hydroxycarbonyl, C:-C4-alkoxycarbonyl, NH(C;-
Cs-alkyl), N(C-C4-alkyl),, phenoxy or phenyl, it being possible for said aryl radicals to be substituted one or more times, e.g. from one to three times by halogen, C:-Cs-alkyl, C-Cg4-haloalkyl, C,-Cs-alkoxy, Ci-Cs-haloalkoxy, R" C,-Cs alkoxycarbonyl, methylenedioxy, ethylenedioxy, C;-C4-alkylthio, phenyl or phenoxy; : phenyl or naphthyl which may be substituted by one or more of the following radicals: halogen, nitro, cyano, hydroxyl, amino, C,-C4-alkyl, C-Cs-haloalkyl,
C,-Ca-alkoxy, C;-C4-haloalkoxy, phenoxy, C4-Ca-alkylthio, NH(C-Cg-alkyl), N(C+-Cs- alkyl); a five- or six-membered heteroaromatic system which contains from one to three nitrogen atoms and/or one sulfur or oxygen atom and which may carry from one to four halogen atoms and/or from one to two of the following radicals: C-Cs4- alkyl, C;-Cs-haloalkyl, C4-C4-alkoxy, C;-Cs-haloalkoxy, Ci-C,-alkyithio, phenyl, phenoxy or phenyicarbonyl, it being possible for the phenyl radicals in turn to carry from one to five halogen atoms and/or from one to three of the following radicals: Cy-Cs-alkyl, C;i-C,-haloalkyl, C1-Cs-alkoxy, C4-Cy-haloalkoxy and/or C;-
C,-alkyithio;
R'® C,-Csalkyl, Ci-Cs-alkoxy, which carry one of the following radicals: hydroxyl, carbamoyl or CON(C4-Cs,-alkyl),; :
Zz sulfur or oxygen.
Particularly preferred compounds of the formula | are those — either as pure enantiomers or pure diastereomers or as mixture thereof — in which the substituents have the following meanings:
R? C,-Csalkyl, C;-Csalkoxy, in particular methyl, ethyl, methoxy, ethoxy, difluoromethoxy, trifluoromethoxy or CR? forms together with CR® a 5-membered alkylene or alkenylene ring which may be substituted by one or two methyl groups and in which in each case one or more methylene groups may be
AMENDED SHEET
® 0050/50501 -12 - replaced by oxygen or sulfur;
R® C-Cs-alkyl, C4-C,-alkoxy, C4-Cs-alkylthio, in particular methyl, ethyl, methoxy, ethoxy, difluoromethoxy, trifluoromethoxy or CR® forms, as indicated for RZ, together with CR? a 5-membered ring; -
R* and R°® phenyl (identical or different) which may be substituted by one or more, e.g. from one to three, of the following radicals: halogen, hydroxyl, C,-Cs-alkyl,
C,-C,-alkoxy, C;-Cy-alkylthio or
R* and R® are phenyl groups which are connected together in ortho positions by a direct linkage, a methylene, ethylene or ethenylene group, an oxygen or sulfur atom or an SO;, NH or N(C,-C,-alkyl) group; or R*and R°® are cyclohexyl;
R® C;-Cg-alkyl, Ca-Cg-alkenyl or Cs-Cg-cycloalkyl, it being possible for each of these radicals to be substituted one or more times by: halogen, hydroxyl, cyano, C4-C,- alkoxy, Cs-Cs-alkenyloxy, C-Cs-alkylthio, phenoxy or phenyl, it being possible for said aryl radicals to be substituted one or more times, e.g. from one to three times, by C;-Cy-alkyl, C¢-C4-alkoxy, methylenedioxy, ethylenedioxy, C;-Cs- alkylthio; phenyl or naphthyl which may be substituted by one or more of the following radicals: halogen, nitro, cyano, hydroxyl, amino, C,-Cs-alkyl, C1-Cs-haloalkyl, C;-
Cs-alkoxy, C4-C4-haloalkoxy, phenoxy, C4-C4-alkylthio, C1-Cq-alkylamino or
C;-C,-dialkylamino; a five- or six-membered heteroaromatic system which contains one nitrogen atom and/or one sulfur or oxygen atom and which may carry from one to four halogen atoms and/or from one to two of the following radicals: C4-Cs-alkyl, C,- : Cg-haloalkyl, C4-C,-alkoxy, C4-Cg-atkylthio, phenyl, phenoxy or phenylcarbonyl, it being possible for the phenyl radicals in turn to carry from one to five halogen atoms and/or from one to three of the following radicals: C4-Cs-alkyl, C4-C,- haloalkyl, C4-C4-alkoxy and/or C,-Cs-alkylthio;
Zz sulfur or oxygen.
" fe
B '0050/50501 -13-
The compounds of the present invention offer a novel therapeutic potential for the treatment of hypertension, pulmonary hypertension, myocardial infarct, angina pectoris, arrhythmia, acute/chronic renal failure, chronic heart failure, renal insufficiency, cerebral vasospasms, cerebral ischemia, subarachnoid hemorrhages, migraine, asthma, atherosclerosis, endotoxic shock, endotoxin-induced organ failure, intravascular coagulation, restenosis after angioplasty and by-pass operations, benign prostate hyperplasia, cirrhosis of the liver, erectile dysfunction, ischemic and intoxication-induced renal failure or hypertension, metastasis and growth of mesenchymal tumors, contrast medium-induced renal failure, pancreatitis, in particular acute pancreatitis, gastrointestinal ulcers.
The invention further relates to combinations of endothelin receptor antagonists of the formula | and inhibitors of the renin-angiotensin system. Inhibitors of the renin- angiotensin system are renin inhibitors, angiotensin Il antagonists and angiotensin converting enzyme (ACE) inhibitors. Combinations of endothelin receptor antagonists of the formula | and ACE inhibitors are preferred.
The invention further relates to combinations of endothelin receptor antagonists of the formula | and beta-blockers.
The invention further relates to combinations of endothelin receptor antagonists of the formula | and diuretics.
The invention further relates to combinations of endothelin receptor antagonists of the formula | and substances which block the action of VEGF (vascular endothelial growth factor). Examples of such substances are antibodies directed against VEGF or specific binding proteins or else low molecular weight substances which are able specifically to inhibit the VEGF release or receptor binding.
The aforementioned combinations may be administered simultaneously or sequentially.
They can be employed either in a single pharmaceutical formulation or else in separate formulations. The form of administration may also differ, for example the endothelin receptor antagonists may be administered orally and the VEGF inhibitors parenterally.
These combination products are particularly suitable for treating and preventing hypertension and its sequelae, and for treating heart failure.
n
AMENDED SHEET
0050/50501 -14-
The good effect of the compounds can be shown in the following tests:
Receptor-binding studies
Cloned human ETa or ETg receptor-expressing CHO cells were employed for binding studies.
Membrane preparation
The ET, or ETs receptor-expressing CHO cells were grown in DMEM NUT MIX Fz medium (Gibco, No. 21331-020) with 10% fetal calf serum (PAA Laboratories GmbH,
Linz, No. A15-022), 1 mM glutamine (Gibco No. 25030-024), 100 U/ml penicillin and 100 ig/ml streptomycin (Sigma No. P-0781). After 48 hours, the cells were washed with PBS and incubated with 0.05% trypsin-containing PBS at 37°C for 5 minutes. This was followed by neutralization with medium, and the cells were collected by centrifugation at 300 x g.
For membrane preparation, the cells were adjusted to a concentration of 10° cells/ml of buffer (50 mM Tris—HCL buffer, pH 7.4) and then disintegrated with ultrasound (Branson
Sonifier 250, 40-70 seconds/constant output 20).
Binding assays :
For the ET, and ET receptor-binding assay, the membranes were suspended in incubation buffer (50 mM Tris—HCI, pH 7.4 with 5 mM MnCl, 40 mg/ml bacitracin and 0.2% BSA) in a concentration of 50 ug of protein per assay mixture and incubated with 25 pM [1251]-ET, (ETA receptor assay) or 25 pM [1251]-ET; (ETg receptor assay) in the presence and absence of test substance at 25°C. The nonspecific binding was determined using 107 M ET,. After 30 min, filtration through GF/B glass fiber filters (Whatman, England) in a Skatron cell harvester (Skatron, Lier, Norway) separated free and bound radio ligand, and the filters were washed with ice-cold Tris—HCI buffer, pH 7.4 with 0.2% BSA. The radioactivity collected on the filters was quantified using a Packard 2200 CA liquid scintillation counter.
i
AMENDED SHEET
® 0050/50501 -15-
Functional vessel test for endothelin receptor antagonists
After pretensioning rabbit aorta segments with 2 g and a relaxation time of 1 h in Krebs-
Henseleit solution at 37°C and a pH between 7.3 and 7.4, initially a contraction is induced with K*. After washing out, an endothelin dose-effect plot is constructed up to the maximum.
Potential endothelin antagonists are administered to other specimens of the same vessel 15 min before starting the endothelin dose-effect plot. The effects of the endothelin are calculated as a % of the K+ contraction. With effective endothelin antagonists there is a rightward shift in the endothelin dose-effect plot.
Testing of ET antagonists in vivo:
Male SD rats weighing 250 — 300 g were anesthetized with amobarbital, artificially ventilated, vagotomized and pithed. The carotid artery and jugular vein were catheterized.
In control animals, intravenous administration of 1 ng/kg ET1 results in a marked rise in blood pressure which persists for a lengthy period.
The test animals received i.v. injection (1 ml/kg) of the test compounds 30 min before administration of ET1. To determine the ET-antagonistic properties, the changes in blood pressure in the test animals were compared with those in the control animals.
Oral testing of mixed ETa and ETg receptor antagonists:
Male normotensive rats (Sprague Dawley, Janvier) weighing 250-350 g are pretreated with the test substances orally. 80 minutes later, the animals are anesthetized with urethane, and the carotid artery (for measuring the blood pressure) and the jugular vein (administration of big endothelin/endothelin 1) are catheterized.
After a stabilization period, big endothelin (20 pg/kg, admin. vol. 0.5 mikg) or ET1 (0.3 ng/kg, admin. vol. 0.5 ml/kg) is given intravenously. Blood pressure and heart rate are recorded continuously for 30 minutes. The marked and long-lasting changes in blood pressure are calculated as the area under the curve (AUC). To determine the antagonistic effect of the test substances, the AUC for the animals treated with
X . ~ 0050/5050 -16 - substance is compared with the AUC for the control animals.
The novel compounds can be administered orally or parenterally (subcutaneously, intravenously, intramuscularly, intraperitoneally) in a conventional way. Administration can also take place with vapors or sprays through the nasopharyngeal space.
The dosage depends on the age, condition and weight of the patient and on the mode of administration. As a rule, the daily dose of active ingredient is from about 0.5 to 50 mg/kg of body weight on oral administration and from about 0.1 to 10 mg/kg of body weight on parenteral administration.
The novel compounds can be administered in conventional solid or liquid pharmaceutical forms, e.g. as uncoated or (fim-)coated tablets, capsules, powders, granules, suppositories, solutions, ointments, creams or sprays. These are produced in a conventional way. The active ingredients can for this purpose be processed with conventional pharmaceutical aids such as tablet binders, bulking agents, preservatives, tablet disintegrants, flow regulators, plasticizers, wetting agents, dispersants, emulsifiers, solvents, release-slowing agents, antioxidants and/or propellant gases (cf. H. Sucker et al.: Pharmazeutische Technologie, Thieme—Verlag, Stuttgart, 1991). The administration forms obtained in this way normally contain from 0.1 to 90% by weight of active ingredient.
Synthesis Examples
Example 1 2-Methylsulfanyl-6,7-dihydro-5H-cyclopentapyrimidine : 4.9 g (44 mmol) of 2-oxocyclopentanecarbaldehyde, dissolved in 100 ml of water, were added over the course of one hour to a solution of 16.4 g of potassium carbonate (119 mmol) and 42.3 g of S-methylisothiourea sulfate (152 mmol) and, after stirring at room temperature overnight, heated at 65°C for 6 hours. The aqueous solution was extracted with pentane, the organic phase was concentrated, and the residue was chromatographed on silica gel (heptane/ethyl acetate 8:2), resulting in 0.93 g of the target compound as a solid.
N, . '0050/50501 -17 -
Example 2 2-Methylsulfonyl-6,7-dihydro-5H-cyclopentapyrimidine
A solution of 8.9 g (16.1 mmol) of Oxone in 70 ml of water and 4M sodium hydroxide solution were added alternately to a solution of 0.85 g (5.1 mmol) of 2-methylsulfanyl- 6,7-dihydro-5H-cyclopentapyrimidine in 20 mi of methanol at 0°C so that a pH of 2-3 was maintained. After the addition was complete, the mixture was stirred at room temperature for 2 hours and then extracted with ethyl acetate, the organic phase was dried over sodium sulfate and evaporated. The solid residue (0.93 g) was employed without further purification.
Example 3
Benzyl 2-(6,7-dihydro-5H-cyclopentapyrimidin-2-yloxy)-3-methoxy-3,3- diphenylpropionate 0.6 g (1.6 mmol) of benzyl 2-hydroxy-3-methoxy-3,3-diphenyl-propionate, dissolved in
DMF, was added dropwise to a suspension of 0.1 g of NaH (3.3 mmol, 80% in white oil) in 10 ml of DMF at 0°C. After the mixture had been stirred for 30 minutes, 420 mg (2.1 mmol) of 2-methylsulfonyl-6,7-dihydro-5H-cyclopentapyrimidine in 10 ml of DMF were added, and the mixture was stirred at room temperature overnight. It was then poured into ice-water and extracted three times with diethyl ether. The ether phases were dried with magnesium sulfate and then filtered, and the solvent was stripped off in vacuo. The yellow residue (0.54 g) was chromatographed on silica gel, allowing 243 mg of the required product to be isolated. :
MS (API): 503 (M+Na)*
Example 4 2-(6,7-Dihydro-5H-cyclopentapyrimidin-2-yloxy)-3-methoxy-3,3-diphenylpropionic acid (I- 136)
A solution of 0.23 g of benzyl 2-(6,7-dihydro-5H-cyclopentapyrimidin-2-yloxy)-3-methoxy- 3,3-diphenylpropionate in 15 ml of ethyl acetate/methanol 2:1 was hydrogenated with hydrogen under atmospheric pressure, using 60 mg of palladium in active carbon (10%),
. 0050/50501 -18 - at room temperature for 24 hours. The mixture was filtered and concentrated, and the residue (177 mg) was stirred into diethyl ether, filtered and then dried. 95 mg of the target product were isolated. 1H-NMR (dg-DMSO, 200 MHz): 8.3 (s, 1H), 7.2-7.4 (m, 10H); 6.15 (s, 1H); 3.3 (s, 3H); 2.8 (m, 4H), 2.1 (m, 2H).
Example 5 2-Chloro-4-methoxy-5-methylpyrimidine
A solution of 25 g of 2,4-dichloro-5-methylpyrimidine in methanol was cooled to 0°C, 28.5 ml of sodium methoxide solution (30% in methanol) were added, and the mixture was stirred firstly at 0°C for one hour and then at room temperature for 2 hours. The resulting suspension was then freed of solvent, taken up in water and extracted with ether. The organic phases were dried over sodium sulfate, filtered and then concentrated, and the resulting residue was chromatographed on silica gel, resulting in 11.4 g of the target compound.
Example 6 2-(4-Methoxy-5-methylpyrimidin-2-yloxy)-3-isopropoxy-3,3-diphenylpropionic acid (I-5) 0.76 g (2.5 mmol) of 2-hydroxy-3-isopropoxy-3,3-diphenylpropionic acid, dissolved in
DMF, was added dropwise to a suspension of 0.23 g of sodium hydride (7.6 mmol, 80% in white oil) in 20 ml of DMF at 0°C. After the mixture had been stirred for 30 minutes, 0.6 g (3.8 mmol) of 2-chloro-4-methoxy-5-methylpyrimidine in 10 ml of DMF was added, then the mixture was stirred firstly at room temperature overnight and then at 40°C for 8 hours. It was then poured into ice-water, adjusted to pH 1 with 2N HCI and extracted three times with diethyl ether. The ether phases were extracted with 1N KOH, and the alkaline aqueous phase was again adjusted to pH 1 with 2N HCI and reextracted with ether. The ether phases obtained in this way were dried over magnesium sulfate and filtered, and the solvent was stripped off in vacuo. The yellowish residue (0.8 g) was chromatographed on silica gel, allowing 0.19 g of the required product to be isolated. 1H-NMR (CDCl3, 200 MHz): 8.0 (s, 1H); 7.5-7.6 (m, 2H); 7.2-7.4 (m, 8H); 6.3 (s, 1H); 3.9 (m, 1H); 3.9 (s, 3H); 2.0 (s, 3H); 1.1 (m, 6H). }
0
AMENDED SHEET
® 0050/50501 -19 -
MS (API): 423 (M+H)*
Example 7 2-Methylsulfanyl-4-methoxy-5-methylpyrimidine 7.2 g (102 mmol) of sodium thiomethanolate were added to a solution of 14.8 g (93 mmol) of 2-chloro-4-methoxy-5-methylpyrimidine in 100 mi of acetonitrile, and the resulting suspension was refluxed for four hours. The solvent was then removed and the residue was taken up in water and extracted with ether. The organic phases were dried over sodium sulfate, filtered and concentrated, and the resulting residue (13.4 g) was reacted without further purification.
Example 8 2-Methylsulfanyl-4-methoxy-5-methylpyrimidine
A solution of 62.4 g (101 mmol) of Oxone in water and 4 M sodium hydroxide solution (about 40 ml) were added to a solution of 13.3 g (78.1 mmol) of 2-methylsulfanyl-4- methoxy-5-methylpyrimidine in 80 mi of methanol at 0°C in such a way that a pH of 2-3 was maintained. After the addition was complete, the mixture was stirred at room temperature for 2 hours and, after removal of methanol, extracted with ethyl acetate, and the organic phase was dried over sodium sulfate and evaporated. The solid residue (14.7 g) was stirred in diethyl ether for two hours, then filtered and dried, resulting in 13.5 g of pure target product.
Example 9 2-(4-Methoxy-5-methylpyrimidin-2-yloxy)-3-benzyloxy-3,3-diphenylpropionic acid (1-47) 1.0 g (2.5 mmol) of 2-hydroxy-3-benzyloxy-3,3-diphenylpropionic acid, dissolved in DMF, was added dropwise to a suspension of 0.27 g of sodium hydride (8 mmol, 80% in white oil) in 20 ml of DMF at 0°C. After stirring the mixture for 30 minutes, 0.79 g (3.9 mmol) of 2-methylsulfonyl-4-methoxy-5-methylpyrimidine in 10 ml of DMF were added, and the . mixture was then stirred at room temperature overnight. It was poured into ice-water adjusted to pH 1 with 2N HCI and extracted three times with diethyl ether. The ether
AMENDED SHEET
@ 0050/50501 - 20 - phases were extracted with IN KOH, and the alkaline aqueous phase was again adjusted to pH 1 with 2N HCI and extracted with ether. The resulting ether phases were dried over magnesium sulfate and filtered, and the solvent was stripped off in vacuo.
The yellowish residue (1.2 g) was mixed with 10 mi of diethyl ether and stirred at room temperature for 3 hours, and then the precipitated solid was filtered off with suction and dried, resulting in 0.6 g of the target compound. 1H-NMR (CDCl3, 200 MHz): 8.0 (s, 1H), 7.2-7.45 (m, 10H); 6.2 (s, 1H); 4.7 (d, 1H); 4.55 (d, 1H); 3.85 (s, 3H); 2.1 (s, 3H).
MS (API): 471 (M+H)*
Example 10 2-(4-Methoxy-5-methylpyrimidin-2-yloxy)-3-hydroxy-3,3-diphenylpropionic acid (I-29)
A solution of 440 mg (0.84 mmol) of 2-(4-methoxy-5-methylpyrimidin-2-yloxy)-3- benzyloxy-3,3-diphenylpropionic acid in 20 ml of ethyl acetate was hydrogenated with hydrogen under atmospheric pressure at room temperature using 80 mg of palladium on active carbon (10%) for 3 days. The mixture was filtered and concentrated, and the residue (430 mg) was chromatographed on silica gel, allowing 32 mg of the desired target product to be isolated. 1H-NMR (ds-DMSO, 200 MHz): 8.0 (s, 1H); 7.6 (m, 2H); 7.0-7.5 (m, 8H); 5.6 (s, 1H); 3.8 (s, 3H); 1.9 (s, 3H).
Example 11 (S5)-2-(4-Methoxy-5-methylpyrimidin-2-yloxy)-3-methoxy-3,3-diphenylpropionic acid (I-2) 10 g (36.7 mmol) of (S)-2-hydroxy-3-methoxy-3,3-diphenylpropionic acid, dissolved in 40 ml of DMF, were added dropwise to a suspension of 3.3 g of sodium hydride (110 mmol, 80% in white oil) in 40 ml of DMF at 0°C. After stirring the mixture for 60 minutes, 9.6 g (47.7 mmol) of 2-methyisulfonyl-4-methoxy-5-methylpyrimidine in 20 ml of DMF were added, and the mixture was then stirred at room temperature overnight. It was poured into ice-water, adjusted to pH 1 with 2N HCI and extracted three times with diethyl ether. The ether phases were extracted with IN KOH, and the alkaline aqueous
} 0050/50501 -21- phase was readjusted to pH 1 with 2N HCI and extracted with ether. The resulting ether phases were dried over sodium sulfate and filtered, and the solvent was stripped off in vacuo. The residue (17.1 g) was stirred in diethyl ether overnight, filtered and dried. The solid (12.1 g) obtained in this way was chromatographed on silica gel, allowing 11.4 g of the desired product to be isolated. 1H-NMR (CDCl3, 270 MHz): 8.0 (s, 1H), 7.2-7.45 (m, 10H); 6.1 (s, 1H); 3.85 (s, 3H); 3.3 (s, 3H); 2.0 (s, 3H). m.p.: 134°C (decomposition)
MS (ESI): 394 (M+H)*
The following compounds were prepared analogously to the abovementioned examples 15.
Example 12 3-Ethoxy-2-(4-methoxy-5-methylpyrimidin-2-yloxy)-3,3-diphenylpropionic acid (1-4) 1H-NMR (CDCl, 200 MHz): 8.0 (s, 1H), 7.1-7.5 (m, 10H); 6.2 (s, 1H); 3.9 (s, 3H); 3.5 (m, 2H); 2.0 (s, 3H); 1.1 (t, 3H).
MS (API): 409 (M+H)*
Example 13 3-[2-(3,4-Dimethoxyphenyl)ethoxy]-2-(4-methoxy-5-methylpyrimidin-2-yloxy)-3,3- diphenylpropionic acid 1H-NMR (CDCls, 200 MHz): 8.0 (s, 1H), 7.1-7.4 (m, 10H); 6.6-6.8 (m, 3H); 6.3 (s, 1H); 3.9 (s, 3H); 3.8 (m, 7H); 3.5-3.65 (m, 1H); 2.7-2.9 (m, 2H); 2.0 (s, 3H); 1.1 (t, 3H).
MS (ESI): 555 (M+H)*
Example 14 3-[2-(3,4-Dimethoxyphenyl)ethoxy]-2-(9-methyl-9H-purin-2-yloxy)-3,3-diphenylpropionic
Claims (19)
1. A compound of the formula } r® B 8 H N AN 2 R—Z—-C—C—0— R lg I = R® R LA I R in which R'is tetrazolyl or a group Oo ll C—R i0 in which R has the following meaning: a) an OR’ radical in which R’ is: hydrogen, the cation of an alkali metal, the cation of an alkaline earth metal, a physiologically tolerated organic ammonium ion such as tertiary C;-C,- alkylammonium or the ammonium ion; Cs-Cg-cycloalkyl, C4-Cg-alkyl, CH»-phenyl which may be substituted by one or more of the following radicals: halogen, nitro, cyano, C;-Cs-alky!, C4-C4-haloalkyt, hydroxyl, C;-Cs4-alkoxy, mercapto, C4-Cs-alkylthio, amino, NH(C,-C,-alkyl), N(C;- Cs-alkyl)z; a Cs-Ce-alkenyl or a C5-Cg-alkynyl group, it being possible for these groups in turn to carry from one to five halogen atoms; R’ can also be a phenyl radical which can carry from one to five halogen atoms and/or from one to three of the following radicals: nitro, cyano, C;-C,-alkyl, C;-C,- haloalkyl, hydroxyl, C,-Cs-alkoxy, mercapto, C;-C;-alkylthio, amino, NH(C4-Cq-alkyl), N(C4-Cy-alkyl),;
. 0050/50501 - 38 - “
b) a 5-membered heteroaromatic system, such as pyrrolyl, pyrazolyl, imidazolyl and triazolyl, which is linked via a nitrogen atom and which may carry from one to two halogen atoms or from one to two C4-Cy-alkyl or from one to two C;-C,-alkoxy groups;
5 C) a group = —Q— (CB,), —8—R’
in which k has the values 0, 1 and 2, p has the values 1, 2, 3 and 4, and R® is C4-Cs-alkyl, Cs-Cg-cycloalkyl, Cs-Ce-alkenyl, Cs-Cg-alkynyl or phenyl which may be substituted by one or more, e.g. from one to three, of the following radicals: halogen, nitro, cyano, C,-Cs-alkyl, C4-C4-haloalkyl, hydroxyl, C;-Cg-alkoxy, C1-Cs-
alkylthio, mercapto, amino, NH(C;-C;-alkyl), N(C4-Cg-alkyl)z;
d) a radical
Lo]
re a s—g’ I 0 in which R? is:
C4-Cs-alkyl, C3-Ces-alkenyl, Cs-Cs-alkynyl, C;-Cg-cycloalkyl, it being possible for these radicals to carry a C,-Cs-alkoxy, C4-C4-alkylthio and/or a phenyl radical as mentioned under c); phenyl which may be substituted by from one to three of the following radicals: halogen, nitro, cyano, C;-Cy-alkyl, C;-C4-haloalkyl, hydroxyl, C;-C4-alkoxy, C4-C,- alkylthio, mercapto, amino, NH(C;-Cy-alkyl), N(C-Cs-alkyl),;
R? is hydroxyl, NHz, NH(C+-Cy-alkvl), N(C1-Cs-alkyl)s, C4-Cs-alkyl, Co-Cs-alkenyl, C,-
N 9050/50501 -39- Cs-alkynyl, C4-C4-hydroxyalkyl, C4-Cy-haloalkyl, C;-Cs-alkoxy, C;-C,-haloalkoxy, C1-C-alkylthio or CR? forms together with CR® a 5- or 6- membered alkylene or alkenylene ring which may be substituted by one or two C;-C,-alkyl groups, in which in each case one or more methylene groups may be replaced by oxygen, sulfur, -NH or —=N(C4-Cy-alkyl);
R® is hydroxyl, NH, NH(C;-Cgs-alkyl), N(C;-C,s-alkyl)z, halogen, C;-Cs-alkyl, Co-Cq- alkenyl, C,-Cy-alkynyl, Cs-Cs-alkenyloxy, C,-C,-alkylcarbonyl, Cy-Cs- alkoxycarbonyl, C,-C,-hydroxyalkyl, C;-Cs-haloalkyl, C;-Cs-alkoxy, C;-Cs-
haloalkoxy, -NH-O-C,-C4-alkyl, C4-C4-alkylthio or CR® forms, as indicated under R?, together with CR? a 5- or 6-membered ring;
R* and R® (which may be identical or different) are:
phenyl or naphthyl, each of which may be substituted by one or more of the following radicals: halogen, nitro, cyano, hydroxyl, C-Cs-alkyl, C4-C4-haloalky!, C4-Cs-alkoxy, C4-Cy4-haloalkoxy, phenoxy, C;-Cs-alkylthio, amino, NH(C4-C,-alkyl), N(C1-C4-alkyl),; or phenyl or naphthyl which are connected together in ortho positions by a direct linkage, a methylene, ethylene or ethenylene group, an oxygen or sulfur atom or an SO,, NH or N-alkyl group; or C3-C-cycloalkyl;
R® is hydrogen,
C4-Cg-alkyl, C3-Cg-alkenyl, C3-Cg-alkynyl or Cs-Cg-cycloalkyl, it being possible for each of these radicals to be substituted one or more times by: hydroxyl,
mercapto, carboxyl, halogen, nitro, cyano, C1-Cs-alkoxy, Cs-Cs-alkenyloxy, Cs-Cs- alkynyloxy, Cs-Cs-alkylthio, C4-Cs-haloalkoxy, C;-Cs-alkylcarbonyl, C;-C,- alkoxycarbonyl, (C4-Cs-alkyl)NHcarbonyl, (Cy-Cs-alkyl).Ncarbonyl, C3-Cs- alkylcarbonylalkyl, amino, NH(C1-Cs-alkyl), N(C,-Cs-alkyl),, phenoxy or phenyl, it being possible for said aryl radicals to be substituted one or more times;
phenyl or naphthyl, each of which may be substituted by one or more of the following radicals: halogen, nitro, cyano, hydroxyl, amino, C;-C4-alkyl, C;-C,-
il AMENDED SHEET 0050/50501 - 40 - haloalkyl, C4-Cq-alkoxy, C;-Cs-haloalkoxy, phenoxy, Ci-Cs-alkylthio, NH(C;-C4- alkyl), N(C4-Cs-alkyl), or methylenedioxy or ethylenedioxy; a five- or six-membered heteroaromatic system which contains from one to three nitrogen atoms and/or a sulfur or oxygen atom and which may carry from one to four halogen atoms and/or from one to two of the following radicals: C,-C,-alkyl, C1-Cs-haloalkyl, C-Cy-alkoxy, C-C4-haloalkoxy, Cq-C,-alkylthio, phenyl, phenoxy or phenylcarbonyl, it being possible for the phenyl radicals in turn to carry from one to five halogen atoms and/or from one to three of the following radicals: C;- Cs-alkyl, C4~C,-haloalkyl, C4-Cs-alkoxy, C4-Cs-haloalkoxy and/or C4-C,-alkylthio; Z is sulfur or oxygen, and the physiologically tolerated salts, tautomeric forms and the enantiomerically pure and diastereomerically pure forms.
2. The use of compounds | as claimed in claim 1 for treating diseases.
3. The use of compounds | as claimed in claim 1 as endothelin receptor antagonists.
4. The use of compounds | as claimed in claim 1 for producing drugs for treating diseases in which elevated endothelin levels occur.
5, The use of compounds | as claimed in claim 1 for producing drugs for treating diseases in which endothelin contributes to the development and/or progression.
6. The use of compounds | as claimed in claim 1 for treating chronic heart failure, restenosis, high blood pressure, pulmonary hypertension, acute/chronic renal failure, cerebral ischemia, asthma, benign prostate hyperplasia, prostate cancer and acute pancreatitis.
7. A combination of compounds | as claimed in claim 1 and one or more active ingredients selected from inhibitors of the renin-angiotensin system such as renin inhibitors, angiotensin II antagonists, angiotensin converting enzyme (ACE) inhibitors, mixed ACE/neutral endopeptidase (NEP) inhibitors, B-blockers, diuretics, calcium channel blockers and VEGF-blocking substances.
0 AMENDED SHEET @ oos0/50501 41.
8. A pharmaceutical preparation for oral or parenteral use, comprising at least one compound | as claimed in claim 1 per single dose, in addition to conventional pharmaceutical excipients.
9. A compound of formula | as claimed in claim 1, substantially as hereinbefore described and exemplified.
10. A compound of formula | including any new and inventive integer or combination of integers, substantially as herein described.
11. The use of compounds of formula | as claimed in any one of claims 2 to 6, substantially as hereinbefore described and exemplified.
12. The use of compounds of formula | including any new and inventive integer or combination of integers, substantially as herein described.
13. A combination of compounds of formula | and one or more active ingredients as claimed in claim 7, substantially as hereinbefore described and exemplified.
14. A combination of compounds of formula | and one or more active ingredients including any new and inventive integer or combination of integers, substantially as herein described.
15. A pharmaceutical preparation as claimed in claim 8, substantially as hereinbefore described and exemplified.
16. A pharmaceutical preparation including any new and inventive integer or combination of integers, substantially as herein described.
17. A compound of formula I, or combination or pharmaceutical preparation comprising thereof as claimed in any one of claims 1, 7 to 10 and 13 to 16 whenever supplied with instructions for the use thereof in the treatment of diseases in which elevated endothelin levels occur, diseases in which endothelin contributes to the development and/or progression, chronic heart failure, restenosis, high blood pressure, pulmonary hypertension, acute/chronic renal failure, cerebral ischemia, asthma, benign prostate hyperplasia, prostate cancer and/or acute pancreatitis.
18. A compound of formula I, or composition or pharmaceutical preparation comprising thereof as claimed in claim 17 when the instructions are in printed or written form.
19. A compound of formula |, or combination or pharmaceutical preparation comprising thereof as claimed in claim 18 supplied in a package or container having the said instructions provided therein or thereon.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19933164A DE19933164A1 (en) | 1999-07-20 | 1999-07-20 | New carboxylic acid derivatives with 5,6 substituted pyrimidine ring, their production and use as endothelin receptor antagonists |
Publications (1)
Publication Number | Publication Date |
---|---|
ZA200200333B true ZA200200333B (en) | 2003-04-30 |
Family
ID=7914884
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ZA200200333A ZA200200333B (en) | 1999-07-20 | 2002-01-15 | Novel carboxylic acid derivatives with 5,6 substituted pyrimidine ring, the production and utilization thereof as endothelin receptor antagonists. |
Country Status (21)
Country | Link |
---|---|
EP (1) | EP1196394A1 (en) |
JP (1) | JP2003505377A (en) |
KR (1) | KR20020019550A (en) |
CN (1) | CN1367778A (en) |
AR (1) | AR030026A1 (en) |
AU (1) | AU6561500A (en) |
BG (1) | BG106321A (en) |
BR (1) | BR0012592A (en) |
CA (1) | CA2379545A1 (en) |
CZ (1) | CZ2002190A3 (en) |
DE (1) | DE19933164A1 (en) |
HU (1) | HUP0202646A3 (en) |
IL (1) | IL147666A0 (en) |
MX (1) | MXPA02000616A (en) |
NO (1) | NO20020254L (en) |
PL (1) | PL353165A1 (en) |
SK (1) | SK772002A3 (en) |
TR (1) | TR200200622T2 (en) |
TW (1) | TW555749B (en) |
WO (1) | WO2001005771A1 (en) |
ZA (1) | ZA200200333B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2462065T3 (en) * | 2009-07-10 | 2014-05-22 | Cadila Healthcare Limited | Improved process for the preparation of Ambrisentan and novel intermediates of this |
WO2011114338A1 (en) | 2010-03-15 | 2011-09-22 | Natco Pharma Limited | A process for the preparation of highly pure ambrisentan |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19636046A1 (en) * | 1996-09-05 | 1998-03-12 | Basf Ag | New carboxylic acid derivatives, their production and use as mixed ET¶A¶ / ET¶B¶ receptor antagonists |
NZ504316A (en) * | 1997-10-31 | 2002-12-20 | Basf Ag | Pyrimidine substituted carboxylic acid derivatives which have amido side-chains useful as endothelin receptor antagonists |
-
1999
- 1999-07-20 DE DE19933164A patent/DE19933164A1/en not_active Withdrawn
-
2000
- 2000-07-05 BR BR0012592-0A patent/BR0012592A/en not_active IP Right Cessation
- 2000-07-05 MX MXPA02000616A patent/MXPA02000616A/en unknown
- 2000-07-05 JP JP2001511432A patent/JP2003505377A/en not_active Abandoned
- 2000-07-05 TR TR2002/00622T patent/TR200200622T2/xx unknown
- 2000-07-05 CA CA002379545A patent/CA2379545A1/en not_active Abandoned
- 2000-07-05 WO PCT/EP2000/006293 patent/WO2001005771A1/en not_active Application Discontinuation
- 2000-07-05 CN CN00810533A patent/CN1367778A/en active Pending
- 2000-07-05 HU HU0202646A patent/HUP0202646A3/en unknown
- 2000-07-05 EP EP00953009A patent/EP1196394A1/en not_active Withdrawn
- 2000-07-05 SK SK77-2002A patent/SK772002A3/en unknown
- 2000-07-05 PL PL00353165A patent/PL353165A1/en not_active Application Discontinuation
- 2000-07-05 KR KR1020027000815A patent/KR20020019550A/en not_active Application Discontinuation
- 2000-07-05 CZ CZ2002190A patent/CZ2002190A3/en unknown
- 2000-07-05 AU AU65615/00A patent/AU6561500A/en not_active Abandoned
- 2000-07-05 IL IL14766600A patent/IL147666A0/en unknown
- 2000-07-13 TW TW089113992A patent/TW555749B/en active
- 2000-07-17 AR ARP000103661A patent/AR030026A1/en unknown
-
2002
- 2002-01-15 ZA ZA200200333A patent/ZA200200333B/en unknown
- 2002-01-17 NO NO20020254A patent/NO20020254L/en not_active Application Discontinuation
- 2002-01-18 BG BG106321A patent/BG106321A/en unknown
Also Published As
Publication number | Publication date |
---|---|
BR0012592A (en) | 2002-05-28 |
BG106321A (en) | 2002-08-30 |
NO20020254D0 (en) | 2002-01-17 |
AR030026A1 (en) | 2003-08-13 |
SK772002A3 (en) | 2003-01-09 |
CN1367778A (en) | 2002-09-04 |
KR20020019550A (en) | 2002-03-12 |
NO20020254L (en) | 2002-02-20 |
JP2003505377A (en) | 2003-02-12 |
IL147666A0 (en) | 2002-08-14 |
HUP0202646A2 (en) | 2003-02-28 |
AU6561500A (en) | 2001-02-05 |
EP1196394A1 (en) | 2002-04-17 |
DE19933164A1 (en) | 2001-01-25 |
PL353165A1 (en) | 2003-10-20 |
TR200200622T2 (en) | 2002-06-21 |
WO2001005771A1 (en) | 2001-01-25 |
HUP0202646A3 (en) | 2003-03-28 |
TW555749B (en) | 2003-10-01 |
CA2379545A1 (en) | 2001-01-25 |
MXPA02000616A (en) | 2002-08-30 |
CZ2002190A3 (en) | 2003-08-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
SK4592000A3 (en) | Novel carboxylic acid derivatives which carry amide side chains, production of said carboxylic acid derivatives and their use as endothelin receptor antagonists | |
SK77799A3 (en) | Heterocyclic carboxylic acid derivatives, the production and use thereof as endothelin receptor antagonists | |
KR20010013981A (en) | New β-Amino and β-Azidocarboxylic Acid Derivatives, the Production Thereof and the Use Thereof as Endothelin Receptor Antagonists | |
ZA200200333B (en) | Novel carboxylic acid derivatives with 5,6 substituted pyrimidine ring, the production and utilization thereof as endothelin receptor antagonists. | |
AU9533398A (en) | Novel carboxylic acid derivatives, their production and their their use as mixed ETa/ETb endothelin-receptor antagonists | |
CA2321182A1 (en) | 5-substituted pyrimidine-2-yloxy carboxylic acid derivatives, the production of the same and their utilization as endothelin antagonists | |
JP2001523671A (en) | Novel substituted α-hydroxycarboxylic acid derivatives, process for their preparation and use as endothelin receptor antagonists | |
JP2002505324A (en) | Novel asymmetrically substituted carboxylic acid derivatives, process for their preparation and use as mixed ETA / ETB receptor antagonists | |
CA2375666A1 (en) | Novel carboxylic acid derivatives comprising aryl-substituted nitrogen heterocycles, their production and their use as endothelin receptor antagonists | |
KR20010101279A (en) | New β-Amide and β-Sulfonamide Carboxylic Acid Derivatives, Their Preparation and Their Use as Endothelin-Receptor Antagonists | |
MXPA99011504A (en) | NEW&bgr;-AMINO AND&bgr;-AZIDOCARBOXYLIC ACID DERIVATIVES, THE PRODUCTION THEREOF AND THE USE THEREOF AS ENDOTHELIN RECEPTOR ANTAGONISTS | |
MXPA00001479A (en) | Novel carboxylic acid derivatives, their production and their use as mixed eta | |
CA2340167A1 (en) | New carboxylic acid derivatives carrying keto side-chains, their production and their use as endothelin-receptor antagonists | |
MXPA00006463A (en) | 5-substituted pyrimidine-2-yloxy carboxylic acid derivatives, the production of the same and their utilization as endothelin antagonists | |
CZ20001582A3 (en) | Novel derivatives of carboxylic acid carrying amide chain process of their preparation and use as endothelin receptor antagonists | |
MXPA01001246A (en) | New carboxylic acid derivatives carrying keto side-chains, their production and their use as endothelin-receptor antagonists | |
CZ20003219A3 (en) | Novel asymmetrically substituted derivatives of carboxylic acids, processes of their preparation and use as mixed antagonists of ETA/ETB receptors | |
CZ20002963A3 (en) | Novel derivatives of carboxylic acid containing 5-substituted pyrimidine ring, process of their preparation and use |