YU141191A - Postupak izolacije restrikcionog fragmentnog brisača u vibrio cholerae i njegovi proizvodi - Google Patents

Postupak izolacije restrikcionog fragmentnog brisača u vibrio cholerae i njegovi proizvodi

Info

Publication number
YU141191A
YU141191A YU141191A YU141191A YU141191A YU 141191 A YU141191 A YU 141191A YU 141191 A YU141191 A YU 141191A YU 141191 A YU141191 A YU 141191A YU 141191 A YU141191 A YU 141191A
Authority
YU
Yugoslavia
Prior art keywords
deletion
vibrio cholerae
chromosome
vivo recombination
wiper
Prior art date
Application number
YU141191A
Other languages
English (en)
Serbian (sr)
Inventor
B.J. Kaper
B. Baudry-Maurelli
A. Fasano
Original Assignee
University Of Maryland At Baltimore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Maryland At Baltimore filed Critical University Of Maryland At Baltimore
Publication of YU141191A publication Critical patent/YU141191A/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)

Abstract

POSTUPAK IZOLACIJE RESTRIKCIONOG FRAGMENTNOG BRISAČA U VIBRIO CHOLERAE I NJEGOVI PROIZVODI, ovaj pronalazak se odnosi na postupke izolovanja mutanata brisanja Vibrio cholerae. U jednom postupku, brisanje je unapred odredjeno razaranjem sa restrikcionim endonukleaza poznate specifičnosti. Brisanja su ubačena u Vibrio cholerae hromozom in vivo rekombinacijom izmedju plazmida koji nosi željeno brisanje, sa susednim flankirajućim sekvenciama i Vibrio cholerae hromozom. U drugom postupku početna in vivo rekombinacija homolognih sekvenci iz rekombinatnog plazmida u hromozomu obezbedjuje selektibilni marker na ovom mestu. Druga in vivo rekombinacija izmedju homolognih flankirajućih sekvenci dovodi do isecanja proficijentnih gena iz hromozoma sa krajnjim proizvodom koji je mutacija brisanja. Pronalazak uključuje izolovanje i karakterizaciju nove Vibrio cholerae vrste koja ima brisanje u ctx genu, kao što je definisano pomoću Acc I, Xba I, Cla I i/ili restrikcionih endonukleaznih mesta i koja dalje ima brisanje u genu koji kodira zonula occludens toksin.
YU141191A 1990-06-05 1991-06-05 Postupak izolacije restrikcionog fragmentnog brisača u vibrio cholerae i njegovi proizvodi YU141191A (sh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US53331590A 1990-06-05 1990-06-05

Publications (1)

Publication Number Publication Date
YU141191A true YU141191A (sh) 1994-06-24

Family

ID=24125419

Family Applications (1)

Application Number Title Priority Date Filing Date
YU141191A YU141191A (sh) 1990-06-05 1991-06-05 Postupak izolacije restrikcionog fragmentnog brisača u vibrio cholerae i njegovi proizvodi

Country Status (13)

Country Link
EP (1) EP0485591B1 (sh)
JP (1) JP3600618B2 (sh)
AT (1) ATE145005T1 (sh)
AU (1) AU656730B2 (sh)
CA (1) CA2064046C (sh)
DE (1) DE69123027T2 (sh)
DK (1) DK0485591T3 (sh)
ES (1) ES2095322T3 (sh)
GR (1) GR3022127T3 (sh)
RU (1) RU2130970C1 (sh)
WO (1) WO1991018979A1 (sh)
YU (1) YU141191A (sh)
ZA (1) ZA914286B (sh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882653A (en) * 1983-03-04 1999-03-16 The University Of Maryland System Vibrio cholerae 01 (CVD111) and non-01 (CVD112 and CVD112RM) serogroup vaccine strains, methods of making same and products thereof
US5874088A (en) * 1992-07-06 1999-02-23 President And Fellows Of Harvard College Deletion mutants of cholera vaccines expressing heterologous antigens
WO1994001533A1 (en) * 1992-07-06 1994-01-20 President And Fellows Of Harvard College Deletion mutants as vaccines for cholera
US6203799B1 (en) 1993-07-01 2001-03-20 Presidents And Fellows Of Harvard College Vibrio cholerae mutants which are soft-agar penetration defective and lack a functional CtxA subunit
AU699912B2 (en) * 1993-10-08 1998-12-17 University Of Maryland At Baltimore (Vibrio cholerae) 01 (CVD111) and non-01 (CVD112 and CVD112RM) serogroup vaccine strains and methods of making same
EP0928831B1 (en) * 1997-12-10 2006-05-03 Council of Scientific and Industrial Research Cholera organisms, vaccines and preparation thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135862A (en) * 1983-03-04 1992-08-04 University Of Maryland Method of isolating restriction fragment deletions in vibrio cholerae, products thereof
US4882278A (en) * 1983-04-29 1989-11-21 President And Fellows Of Harvard College Non-toxinogenic vibrio cholerae mutants

Also Published As

Publication number Publication date
CA2064046A1 (en) 1991-12-06
WO1991018979A1 (en) 1991-12-12
DE69123027T2 (de) 1997-03-13
CA2064046C (en) 1999-04-13
AU8214791A (en) 1991-12-31
DK0485591T3 (da) 1997-04-07
ATE145005T1 (de) 1996-11-15
ES2095322T3 (es) 1997-02-16
EP0485591B1 (en) 1996-11-06
AU656730B2 (en) 1995-02-16
JP3600618B2 (ja) 2004-12-15
DE69123027D1 (de) 1996-12-12
EP0485591A4 (en) 1993-07-14
EP0485591A1 (en) 1992-05-20
RU2130970C1 (ru) 1999-05-27
JPH05502381A (ja) 1993-04-28
ZA914286B (en) 1992-03-25
GR3022127T3 (en) 1997-03-31

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