Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01C—PLANTING; SOWING; FERTILISING
  • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
  • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
  • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
  • A01P21/00—Plant growth regulators

Definitions

• the present invention relates to a method for treating plants, a formulation, plant propagules, a method for growing plants, and transplants.
• Patent Document 1 describes that glycine betaine has the effect of alleviating abiotic stress on plants, such as insufficient irrigation.
• Patent Document 2 also describes that applying an aqueous solution of ergothioneine to germinated plants increases plant height, the number of flowers and fruits, and the seed yield.
• Abiotic stress is thought to be a major hindrance to the expression of a plant's potential productivity. Therefore, it is expected that the production volume of plants will increase dramatically by providing biostimulants that eliminate abiotic stress.
• the present invention has been made in consideration of the above problems, and aims to provide a method for treating plants that can further promote plant growth using compounds such as ergothioneine, a formulation used in said method, propagation material of a plant treated with said method, a method for growing plants from said propagation material, and transplants grown from said propagation material.
• a method for treating a plant comprising the step of treating a propagation material of the plant with an active ingredient which is a compound represented by the following formula (I) or a tautomer thereof, or an agriculturally acceptable salt thereof:
• R1 and R2 independently represent a hydrogen atom or an alkyl group having 1 to 4 carbon atoms
• R3 , R4 , and R5 independently represent an alkyl group having 1 to 4 carbon atoms.
• the active ingredient is a growth promoter.
• R1 and R2 independently represent a hydrogen atom or an alkyl group having 1 to 4 carbon atoms, and R3 , R4 , and R5 independently represent an alkyl group having 1 to 4 carbon atoms.
• the active ingredient is a growth promoter.
• a plant propagation material comprising an active ingredient which is a compound represented by the following formula (I) or a tautomer thereof, or an agriculturally acceptable salt thereof:
• R1 and R2 independently represent a hydrogen atom or an alkyl group having 1 to 4 carbon atoms
• R3 , R4 , and R5 independently represent an alkyl group having 1 to 4 carbon atoms.
• the active ingredient is a growth promoter.
• the active ingredient is attached to the surface of the propagule.
• the propagules contain an active ingredient which is a compound of formula (I) or a tautomer thereof, or an agriculturally acceptable salt thereof: How to grow plants.
• R1 and R2 independently represent a hydrogen atom or an alkyl group having 1 to 4 carbon atoms
• R3 , R4 , and R5 independently represent an alkyl group having 1 to 4 carbon atoms.
• the active ingredient is a growth promoter.
• Prior to the germination step treating said propagules with said active ingredient; The method for cultivating a plant according to [11] or [12].
• [14] A transplanted seedling grown by the method according to any one of [11] to [13].
• the present invention provides a method for treating plants that can further promote plant growth using compounds such as ergothioneine, a formulation for use in the method, propagation material of a plant treated with the method, a method for growing a plant from the propagation material, and a transplant grown from the propagation material.
• One embodiment of the present invention relates to a method for treating seeds, comprising the step of treating plant seeds with an active ingredient which is a compound represented by the following formula (I) or a tautomer thereof, or an agriculturally acceptable salt thereof:
• R 1 and R 2 independently represent a hydrogen atom or an alkyl group having 1 to 4 carbon atoms
• R 3 , R 4 and R 5 independently represent an alkyl group having 1 to 4 carbon atoms.
• the alkyl group which can be represented by R 1 to R 5 may be linear or branched.
• Examples of the alkyl group which can be represented by R 1 to R 5 include a methyl group, an ethyl group, a propyl group, an isopropyl group, a butyl group, an isobutyl group, a sec-butyl group, and a tert-butyl group.
• At least one of R1 and R2 is preferably a hydrogen atom, and more preferably both are hydrogen atoms.
• R1 and R2 are alkyl groups, they are preferably methyl, ethyl, or propyl, more preferably methyl or ethyl, and even more preferably methyl.
• R3 , R4 and R5 are preferably each independently a methyl group, an ethyl group, or a propyl group, more preferably a methyl group or an ethyl group, and even more preferably a methyl group.
• R3 , R4 and R5 it is preferable that at least one is a methyl group, more preferably at least two are methyl groups, and even more preferably all are methyl groups.
• the compound represented by formula (I) has a tautomer when at least one of R 1 and R 2 is a hydrogen atom. More specifically, the compound represented by formula (I) has a tautomer represented by the following formula (II) when R 2 is a hydrogen atom. In addition, the compound represented by formula (I) has a tautomer represented by the following formula (III) when R 1 is a hydrogen atom. In this specification, when the term "tautomer” is used simply, it means both the compound represented by formula (II) and the compound represented by formula (III).
• R 1 to R 5 are the same as R 1 to R 5 in formula (I).
• the compound represented by formula (I) and the compound represented by formula (II) or the compound represented by formula (III) can exist in equilibrium.
• the ratio of the compound represented by formula (I) to the compound represented by formula (II) or the compound represented by formula (III) can vary depending on the solvent, temperature, pH, etc.
• the compound represented by formula (I) or its tautomer is preferably ergothioneine, more preferably L-(+)-ergothioneine.
• Ergothioneine is also known to be produced by bacteria and fungi. Examples of production methods using such microorganisms include those described in JP-A-2012-105618, JP-A-2014-223051, WO-2016/104437, WO-2016/121285, WO-2015/168112, and WO-2017/150304. Ergothioneine may be used as a culture containing ergothioneine obtained from these microorganisms, or ergothioneine obtained by concentrating or purifying the above culture may be used.
• Agriculturally acceptable means safe, non-toxic, and not biologically or otherwise undesirable, and is acceptable for use in agricultural and horticultural applications, particularly as an agricultural and horticultural agent for promoting growth and improving plant resistance to abiotic stresses.
• Agriculturally acceptable salt of a compound represented by formula (I) or a tautomer thereof means an agriculturally acceptable salt as defined above that provides the action and effect of a compound represented by formula (I) or a tautomer thereof.
• Examples of such salts include hydrates, solvates, acid addition salts, salts formed by replacing an acidic proton present in a compound represented by formula (I) or a tautomer thereof with a metal ion, and salts formed by coordinating the acidic proton with an organic base or an inorganic base.
• Acid addition salts may be formed with inorganic acids or organic acids.
• inorganic acids include hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, and phosphoric acid.
• organic acids include acetic acid, benzenesulfonic acid, benzoic acid, camphorsulfonic acid, citric acid, ethanesulfonic acid, fumaric acid, glucoheptonic acid, gluconic acid, glutamic acid, glycolic acid, hydroxynaphthoic acid, 2-hydroxyethanesulfonic acid, lactic acid, maleic acid, malic acid, mandelic acid, methanesulfonic acid, muconic acid, 2-naphthalenesulfonic acid, propionic acid, salicylic acid, succinic acid, dibenzoyl-L-tartaric acid, tartaric acid, p-toluenesulfonic acid, trimethylacetic acid, and trifluoroacetic acid.
• metal ions that can replace the acidic protons present in the compound of formula (I) or its tautomer include alkali metal ions, alkaline earth metal ions, and aluminum ions.
• Examples of organic bases that can coordinate with the acidic protons present in the compound represented by formula (I) or its tautomers include diethanolamine, ethanolamine, N-methylglucamine, triethanolamine, and tromethamine.
• Examples of inorganic bases that can coordinate with the acidic protons present in the compound represented by formula (I) or its tautomers include aluminum hydroxide, calcium hydroxide, potassium hydroxide, sodium carbonate, and sodium hydroxide.
• the above active ingredients are thought to act as so-called biostimulants, promoting plant growth and mitigating abiotic stress. As will be confirmed in the examples described below, the above active ingredients have excellent growth-promoting effects and are therefore useful as growth promoters.
• Methods of treating plants with the above-mentioned active ingredients include spraying on stems and leaves, introduction into water supply, spraying on soil, injection into subsoil using an injection machine, and treatment of propagules such as seeds, bulbs and tubers.
• treatment of propagules is superior to other treatment methods in terms of plant growth promotion effect.
• excellent growth promotion effect refers to at least one of the plant growth indicators being superior to other treatment methods.
• plant growth indicators include, for example, plant height, above-ground weight, below-ground weight, tiller number, root yield, stem yield, leaf number, leaf yield, flower bud number, flower number, fruit number, fruit yield, seed number and seed yield.
• treatment of propagules can fully obtain these effects even with a smaller amount than when treating other organs.
• the treatment of propagules means a treatment in which a treatment agent containing the active ingredient is directly brought into contact with the propagules.
• the treatment agent is an agent used to treat the propagules.
• the treatment agent may be the active ingredient itself, or a formulation containing the active ingredient in the form distributed on the market may be used as the treatment agent as is, or a formulation containing the active ingredient may be diluted or mixed with other ingredients as necessary at the time of treatment to prepare the treatment agent.
• the application of a treatment agent containing the active ingredient to the soil or medium in which the propagules are sown or placed is not included in the treatment of the propagules.
• treatment methods using a liquid treatment agent include application of the treatment liquid by a method such as spraying, application of the active ingredient to the propagules by mixing with the treatment liquid, immersion in the treatment liquid, and contacting the propagules with a sponge or cotton cloth containing the treatment liquid to bring the treatment agent into contact with the propagules.
• treatment methods using a solid treatment agent include a method of spraying a powdered treatment agent on the propagules, and a method of dusting the propagules with a powdered treatment agent.
• the propagules may be coated with pellets (clay mineral) containing the active ingredient, or the propagules may be coated with a resin containing the active ingredient.
• the resin used for the coating may be in the form of a film, may be dissolved or dispersed in a solvent, or may be in other forms.
• the propagation material is preferably treated in a state where the above treatment is possible, for example, where the propagation material can be sprayed, applied, dipped, dusted or coated with a treatment agent.
• a treatment agent for example, it is preferable to treat the propagation material before cultivation of the propagation material begins (for example, before sowing).
• the propagation material may be in a dormant state where growth has stopped, or in a non-dormant state where growth has begun.
• the temperature during treatment (e.g., the temperature of the treatment agent) is preferably between 0°C and 80°C, and more preferably room temperature.
• the treated propagules are preferably dried to remove the liquid carrier.
• the above-mentioned plants may be any of wild plants, plant cultivars, plants and plant cultivars obtained by conventional biological breeding such as crossbreeding or protoplast fusion, and genetically modified plants and plant cultivars obtained by genetic engineering.
• genetically modified plants and plant cultivars obtained by genetic engineering include herbicide-resistant crops, pest-resistant crops incorporating an insecticidal protein-producing gene, disease-resistant crops incorporating a disease-resistance inducer-producing gene, crops with improved taste, crops with improved yield, crops with improved storability, and crops with improved yield.
• Examples of genetically modified plant cultivars approved in various countries include the various cultivars stored in the database of the International Society for Agricultural Biotechnology (ISAA).
• the active ingredient can be distributed on the market as a formulation.
• the active ingredient itself can be formulated, or it can be formulated in various forms together with other adjuvants or other active ingredients.
• the formulation form is not particularly limited and may be selected according to the treatment method. Examples of formulation forms include dusts, granules, powders, hydrates, water-soluble concentrates, emulsions, liquids, oils, aerosols, microcapsules, pastes, liniments, smoking agents, fumigants, and microdispersants.
• the carrier may be a solid carrier or a liquid carrier.
• solid carriers include minerals such as clay, talc, diatomaceous earth, zeolite, montmorillonite, bentonite, kaolinite, kaolin, pyrophyllite, rosewood, acid clay, activated clay, attapulgite, attapulgus clay, limestone, calcite, marble, vermiculite, perlite, pumice, silica stone, silica sand, sericite, and pottery stone; synthetic organic substances such as urea; salts such as calcium carbonate, sodium carbonate, magnesium carbonate, sodium sulfate, ammonium sulfate, potassium chloride, hydrated lime, and sodium bicarbonate; amorphous silica (white carbon, fumed silica, etc.) and distillates.
• minerals such as clay, talc, diatomaceous earth, zeolite, montmorillonite, bentonite, kaolinite, kaolin, pyrophyllite, rosewood, acid clay, activated clay, attapul
• powdered and granular carriers such as synthetic inorganic substances such as titanium oxide, plant-based carriers such as wood flour, corn stalks (cobs), walnut shells (nut husks), fruit kernels, rice husks, coconut shells, sawdust, bran, soy flour, powdered cellulose, starch, dextrin, and sugars (lactose, sucrose, etc.), as well as various polymeric carriers such as cross-linked lignin, cationic gels, gelatin that gels when heated or treated with polyvalent metal salts, water-soluble polymer gels (agar, etc.), chlorinated polyethylene, chlorinated polypropylene, polyvinyl acetate, polyvinyl chloride, ethylene/vinyl acetate copolymers, and urea/aldehyde resins.
• synthetic inorganic substances such as titanium oxide
• plant-based carriers such as wood flour, corn stalks (cobs), walnut shells (nut husks), fruit kernels,
• liquid carriers examples include aliphatic solvents such as paraffins (normal paraffin, isoparaffin, naphthene), aromatic solvents such as xylene, alkylbenzene, alkylnaphthalene and solvent naphtha, mixed solvents such as kerosene, machine oils such as refined high-boiling aliphatic hydrocarbons, alcohols such as methanol, ethanol, isopropanol, butanol and cyclohexanol, polyhydric alcohols such as ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol, hexylene glycol, polyethylene glycol and polypropylene glycol, polyhydric alcohol derivatives such as propylene glycol ethers, acetone, acetophenone, cyclohexanone, methylcyclohexanone and These include ketones such as ⁇ -butyrolactone, fatty acid methyl esters (coconut oil fatty acid methyl esters),
• the surfactant may be any of a variety of surfactants, including nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, silicone surfactants, fluorine-based surfactants, and biosurfactants.
• nonionic surfactants examples include sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, sucrose fatty acid esters, polyoxyethylene fatty acid esters, polyoxyethylene resin acid esters, polyoxyethylene fatty acid diesters, polyoxyethylene alkyl ethers, polyoxyethylene alkylphenyl ethers, polyoxyethylene dialkylphenyl ethers, polyoxyethylene alkylphenyl ether formalin condensates, polyoxyethylene/polyoxypropylene block polymers, alkyl polyoxyethylene/polyoxypropylene block polymer ethers, alkylphenyl polyoxyethylene/polyoxypropylene block polymer ethers, polyoxyethylene alkylamines, polyoxyethylene fatty acid amides, polyoxyethylene fatty acid bisphenyl ethers, polyoxyethylene benzyl phenyl (or phenyl phenyl) ethers, polyoxyethylene styryl phenyl (or phenyl
• anionic surfactants include sulfates such as alkyl sulfate, polyoxyethylene alkyl ether sulfate, polyoxyethylene alkyl phenyl ether sulfate, polyoxyethylene benzyl (or styryl) phenyl (or phenylphenyl) ether sulfate, and polyoxyethylene/polyoxypropylene block polymer sulfate, paraffin (alkane) sulfonate, ⁇ -olefin sulfonate, dialkyl sulfosuccinate, alkylbenzene sulfonate, mono- or dialkylnaphthalene sulfonate, naphthalene sulfonate-formaldehyde condensate, alkyl diphenyl ether disulfonate, lignin sulfonate, polyoxyethylene alkyl ether sulfate ...
• sulfates
• sulfonates such as diethylene alkyl phenyl ether sulfonate and polyoxyethylene alkyl ether sulfosuccinic acid half ester
• carboxylates such as fatty acids, resin acids, polycarboxylic acids, alkyl ether carboxylates, alkenyl succinic acids, N-acyl amino acids and naphthenic acids
• phosphates such as polyoxyethylene alkyl ether phosphate, polyoxyethylene mono- or dialkyl phenyl ether phosphate, polyoxyethylene benzyl (or styryl) phenyl (or phenyl phenyl) ether phosphate, polyoxyethylene/polyoxypropylene block polymer phosphate and alkyl phosphate.
• cationic surfactants include salts of amines such as alkylamines and alkylpentamethylpropylenediamines, as well as salts of ammoniums such as alkyltrimethylammonium, methylpolyoxyethylenealkylammonium, alkylpyridinium, mono- or dialkylmethylated ammonium, alkyldimethylbenzalkonium, and benzethonium (octylphenoxyethoxyethyldimethylbenzylammonium).
• amines such as alkylamines and alkylpentamethylpropylenediamines
• ammoniums such as alkyltrimethylammonium, methylpolyoxyethylenealkylammonium, alkylpyridinium, mono- or dialkylmethylated ammonium, alkyldimethylbenzalkonium, and benzethonium (octylphenoxyethoxyethyldimethylbenzylammonium).
• amphoteric surfactants examples include dialkyldiaminoethyl betaine, alkyldimethylbenzyl betaine, and lecithin (phosphatidylcholine, phosphatidylethanolamine, etc.).
• silicone surfactants examples include trisiloxane ethoxylate.
• fluorosurfactants include perfluoroalkyl carboxylates, perfluoroalkyl sulfonates, and perfluoroalkyl trimethyl ammonium salts.
• biosurfactants examples include sophorolipids, rhamnolipids, trehalose lipids, mannosylalditol lipids, cellobiose lipids, glucose lipids, oligosaccharide fatty acid esters, spiculesporic acid, corynomycolic acid, agaritic acid, surfactin, serawettin, viscosin, lykensin, arthrofactin, emulsan, and alasan.
• auxiliary agents include inorganic salts (sodium, potassium, etc.) used as pH adjusters, water-soluble salts such as table salt, xanthan gum, guar gum, carboxymethylcellulose, polyvinylpyrrolidone, carboxyvinyl polymers, acrylic polymers, polyvinyl alcohol, starch derivatives, water-soluble polymers (polysaccharides, etc.), alginic acid and its salts, etc. used as thickeners, metal stearates, sodium tripolyphosphate, sodium hexametaphosphate, etc.
• inorganic salts sodium, potassium, etc.
• water-soluble salts such as table salt, xanthan gum, guar gum, carboxymethylcellulose, polyvinylpyrrolidone, carboxyvinyl polymers, acrylic polymers, polyvinyl alcohol, starch derivatives, water-soluble polymers (polysaccharides, etc.), alginic acid and its salts, etc. used as thickeners, metal
• disintegrating and dispersing agents benzoic acid and its salts, sorbic acid and its salts, propionic acid and its salts, p-hydroxybenzoic acid, methyl p-hydroxybenzoate, 1,2-benzothiazolin-3-one, etc. used as preservatives, and supplements.
• active ingredients include those contained in biostimulants, plant growth regulators, fungicides, insecticides, acaricides, nematicides and herbicides.
• the above active ingredients can act as so-called biostimulants that mitigate abiotic stress and promote plant growth, but by using them in combination with other biostimulants, it is possible to further increase resistance to abiotic stress and further increase the growth promotion effect.
• biostimulants examples include seaweed extract, corn extract, microalgae, mycorrhizal fungi, humic acid, fulvic acid, oxidized glutathione, L-proline, glycine betaine, 5-aminolevulinic acid, 2-hexenal, trehalose, silicic acid, nicotinic acid, acetic acid, and ethanol.
• Examples of the above plant growth regulators include aminoethoxyvinylglycine, chlormequat, chlorpropham, cyclanilide, dikeglac, daminozide, ethephon, flurprimidol, flumetralin, forchlorfenuron, gibberellin, mepiquat chloride, methylcyclopropene, benzylaminopurine, paclobutrazol, prohexadione, thidiazuron, tributyl phosphorotrithioate, trinexapac-ethyl, uniconazole, sodium 1-naphthalene acetate, These include 1-naphthylacetamide, 1-methylcyclopropene, 4-CPA (4-chlorophenoxyacetic acid), MCPB (ethyl 2-methyl-4-chlorophenoxybutyrate), isoprothiolane, indolebutyric acid, ethychlozate, calcium formate, chlormequat,
• fungicides examples include nucleic acid synthesis metabolic inhibitors, fungicides that act on the cytoskeleton and motor proteins, respiratory inhibitors, amino acid and protein biosynthesis inhibitors, signal transduction inhibitors, lipid biosynthesis or transport/cell membrane structure or function inhibitors, cell membrane sterol biosynthesis inhibitors, cell wall biosynthesis inhibitors, melanin biosynthesis inhibitors, host plant resistance inducers, multi-site fungicides, and biological pesticides/biologically derived pesticides with multiple modes of action.
• nucleic acid synthesis metabolic inhibitors include benalaxyl, benalaxyl M or chiralaxyl, furalaxyl, metalaxyl, metalaxyl M or mefenoxam, ofurace, oxadixyl, bupirimate, dimethirimol, ethirimol, hydroxyisoxazole, octhilinone, and oxolinic acid.
• amino acid and protein biosynthesis inhibitors examples include cyprodinil, mepanipyrim, pyrimethanil, blasticidin S, kasugamycin, streptomycin, and oxytetracycline.
• melanin biosynthesis inhibitors examples include fthalide, pyroquilon, tricyclazole, carpropamid, diclocymet, fenoxanil, and tolprocarb.
• the above-mentioned host plant resistance inducers include acibenzolar-S-methyl, probenazole, tiadinil, isotianil, laminarin, Japanese knotweed extract, Bacillus mycoides isolate J, cell wall of Saccharomyces cerevisiae strain LAS117, fosetyl (fosetyl-aluminum, fosetyl potassium, fosetyl sodium), phosphoric acid, phosphate salts, and diclobentiazox.
• multi-site fungicides include ferbam, mancozeb, maneb, metiram, propineb, thiuram, zinc thiazole, zineb, ziram, ambam, anilazine, dithianon, dichlofluanid, tolylfluanid, guazatine, iminoctadine acetate, iminoctadine albesilate, copper or various copper salts (e.g., basic copper chloride, cupric hydroxide, basic copper sulfate, copper sulfate, organocupric (copper oxine), copper nonylphenolsulfonate, DBEDC, etc.), sulfur, captan, captafol, folpet, TPN (chlorothalonil), quinoxalines (quinomethionate), fluorimide, and metasulfocarb.
• copper salts e.g., basic copper chloride, cupric hydroxide, basic copper sulfate
• biological pesticides/biological pesticides having multiple action mechanisms include Bacillus subtilis AFS032321 strain, Bacillus amyloliquefaciens QST713 strain, Bacillus amyloliquefaciens FZB24 strain, Bacillus amyloliquefaciens MBI600 strain, Bacillus amyloliquefaciens D747 strain, Bacillus amyloliquefaciens F727 strain, Clonostachys rosea CR-7 strain, Gliocladium catenara Tam J1446 strain, Pseudomonas chlororaphis AFS009 strain, Streptomyces griseoviridis K61 strain, Streptomyces ridix WYEC108 strain, Trichoderma atroviride I-1237 strain, Trichoderma atroviride LU132 strain, Trichoderma atroviride SC1 strain, Trichoderma asperellum T34 strain, Swaingrea glutinosa extract, and extract from cotyled
• compounds for fungicide use include chlorinconazid, seboctylamin, flumethylsulfolim, flufenoxadiazam, cyflufenamid, cymoxanil, diclomedine, dipimethitron, dodine, fenitropan, ferimzone, flusulfamide, flutianil, harpin, inorganic salts (bicarbonates (sodium bicarbonate, potassium bicarbonate), potassium carbonate), ipflufenoquin, kinoprol, natural product origin, machine oil, organic oil, picarbutrazox, pyridaclomethyl, quinofumelin, tebufloquine, tecloftalam (bactericide), triazoxide, validamycin, aminopyrifen and shiitake mushroom mycelium extract.
• insecticides include acetylcholinesterase (AChE) inhibitors, GABAergic chloride channel blockers, sodium channel modulators, nicotinic acetylcholine receptor (nAChR) competitive modulators, nicotinic acetylcholine receptor (nAChR) allosteric modulators, glutamatergic chloride channel (GluCl) allosteric modulators, juvenile hormone analogues, other non-specific (multi-site) inhibitors, chordotonal organ TRPV channel modulators, mite growth inhibitors acting on CHS1, microbial insect midgut membrane disruptors, mitochondrial ATP synthase inhibitors, oxidative phosphorylation uncouplers that disrupt the proton gradient, nicotinic acetylcholine receptor (AChR ...nicotinic acetylcholine receptor (AChR) allosteric modulators, nicotinic acetylcholine receptor (AChR)
• acetylcholinesterase (AChE) inhibitors include alanycarb, aldicarb, bendiocarb, benfuracarb, butocarboxim, butoxycarboxim, NAC (carbaryl), carbofuran, carbosulfan, ethiofencarb, BPMC (fenobucarb), fenothiocarb, formetanate, furathiocarb, MIPC (isoprocarb), methiocarb, methomyl, MTMC (metolcarb), oxamyl, pirimicarb, PH C (propoxur), thiodicarb, thiofanox, triazamate, trimethacarb, XMC, MPMC (xylylcarb), acephate, azamethiphos, azinphos ethyl, azinphos methyl, cadusafos, chlorethoxyphos, CVP (chlor
• GABA-gated chloride ion channel blockers examples include chlordane, benzoepine (endosulfan), dienochlor, ethiprole, fipronil, pyriprol, and nicoflurane.
• sodium channel modulators examples include acrinathrin, allethrin (allethrin, d-cis-trans-, d-trans-isomers), bifenthrin, bioallethrin (bioallethrin, S-cyclopentenyl-isomer), bioresmethrin, chloroprallethrin, chlorfenthone, cycloprothrin, cyfluthrin (cyfluthrin, ⁇ -isomer), cyhalothrin (cyhalothrin, ⁇ -, ⁇ -isomers), cypermethrin (cypermethrin, ⁇ -, ⁇ -, ⁇ -, ⁇ -isomers), cyphenothrin [(1R)-trans isomer], deltamethrin, dimefluthrin, empenthrin [(EZ)-(1R)-isomer], esfenvalerate, a
• nicotinic acetylcholine receptor (nAChR) competitive modulators include acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, thiamethoxam, nicotine sulfate (nicotine), sulfoxaflor, flupyradifurone, dichloromezothiaz, phenmezodithiaz, and triflumezopyrim.
• nicotinic acetylcholine receptor (nAChR) allosteric modulators examples include spinetoram, spinosad, flupirimine and GS-omega/kappa HXTX-Hv1a peptide.
• glutamate-gated chloride channel (GluCl) allosteric modulators examples include abamectin, emamectin benzoate, lepimectin, and milbemectin.
• mite growth inhibitors that act on the above-mentioned CHS1 include clofentezine, diflobidazine, hexythiazox, and etoxazole.
• insect midgut membrane disrupting agents examples include Bacillus thuringiensis subsp. israelensis, Bacillus thuringiensis subsp. aizawai, Bacillus thuringiensis subsp. kurstaki, Bacillus thuringiensis subsp. tenebrionis, B. t. These include proteins found in crops (Cry1Ab, Cry1Ac, Cry1Fa, Cry1A.105, Cry2Ab, Vip3A, mCry3A, Cry3Bb, Cry34Ab1/Cry35Ab1) and Bacillus sphaericus.
• mitochondrial ATP synthase inhibitors examples include diafenthiuron, azocyclotin, tricyclohexyltin hydroxide (cyhexatin), fenbutatin oxide, BPPS (propargite), and tetradifon.
• nicotinic acetylcholine receptor (nAChR) channel blockers examples include bensultap, cartap hydrochloride, thiocyclam, thiosultap sodium salt, and monosultap.
• Examples of chitin biosynthesis inhibitors that act on the above-mentioned CHS1 include bistrifluron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, noviflumuron, teflubenzuron, and triflumuron.
• Examples of the chitin biosynthesis inhibitors include buprofezin, etc.
• molting inhibitors examples include cyromazine, etc.
• mitochondrial electron transport chain complex III inhibitors examples include hydramethylnon, acequinocyl, fluacrypyrim, flupiroxystrobin, and bifenazate.
• mitochondrial electron transport complex I inhibitors examples include fenazaquin, fenpyroximate, pyridaben, pyrimidifen, tebufenpyrad, tolfenpyrad, and delis (rotenone).
• Examples of voltage-dependent sodium channel blockers include indoxacarb and metaflumizone.
• the above-mentioned mitochondrial electron transport chain complex IV inhibitors include aluminum phosphide, calcium phosphide, hydrogen phosphide, zinc phosphide, hydrocyanic acid (calcium cyanide, sodium cyanide), and potassium cyanide.
• mitochondrial electron transport chain complex II inhibitors examples include cyenopyrafen, sietopyrafen, cyflumetofen, piflubumid, and cyclobutrifluram.
• chordotonal organ modulators examples include flonicamide, etc.
• GABA-gated chloride ion channel allosteric modulators examples include broflanilide, fluxamethamide, and isocycloceram.
• baculovirus examples include the codling moth Cydia pomonella GV, the false codling moth Thaumatotibia leucotreta GV, the velvet bean caterpillar Anticarsis gemmatalis MNPV, and the cotton bollworm Helicoverpa armigera NPV.
• insecticides include azadirachtin, benzomate (benzoximate), phenisobromorate (bromopropylate), quinoxalines (quinomethionate), kelthane (dicofol), lime sulfur, mancozeb, pyridalyl, sulfur, acinonapyr, amidoflumet, benzpyrimoxane, fluazaindolizine, fluensulfone, fluhexafon, flupentiofenox, flometoquin, metaldehyde, cyclopyrazoflor, zipropyridaz, trifluenfuronate, indazapiroxamet, sulfiflumine, bisulfulfen, isoflualanum, pi
• perfuranilide Burkholderia spp., Wolbachia pipientis (Zap), Atractylodes macrocarpa extract, fatty acid monoesters
• Examples of the above herbicides include acetolactate synthase (ALS) inhibitor compounds, amino acid compounds, cyclohexanedione compounds, acetamide compounds, bipyridylium compounds, allyloxyphenoxypropionic acid compounds, carbamate compounds, pyridine compounds, urea compounds, dinitroaniline compounds, protoporphyrinogen oxidase (PPO) inhibitor compounds, phenoxyacetic acid compounds, hydroxyphenylpyruvate dioxygenase enzyme (HPPD) inhibitor compounds, and triazine compounds.
• ALS acetolactate synthase
• amino acid compounds amino acid compounds
• cyclohexanedione compounds cyclohexanedione compounds
• acetamide compounds bipyridylium compounds
• allyloxyphenoxypropionic acid compounds carbamate compounds
• pyridine compounds urea compounds
• dinitroaniline compounds protoporphyrinogen oxidase (PPO)
• examples of the acetolactate synthesis (ALS) inhibitor compounds include imazamethabenz and imazamethabenz-methyl, imazamox, imazapic, imazapyr, imazaquin, imazethapyr, amidosulfuron, azimsulfuron, bensulfuron and bensulfuron-methyl, chlorimuron and chlorimuron-methyl, chlorimuron-ethyl, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron and ethametsulfuron.
• imazamethabenz and imazamethabenz-methyl imazamox, imazapic, imazapyr, imazaquin, imazethapyr, amidosulfuron, azimsulfuron, bensulfuron and bensulfuron-methyl, chlorimuron and chlorimuron-methyl, chlorimuron-ethyl, chlorsulfuron, cinosulfuron
• amino acid compounds examples include bialaphos and its salts, glufosinate and its salts, glufosinate P and its salts, and glyphosate and its salts.
• cyclohexanedione compounds examples include alloxydim, butroxydim, clethodim, cloproxydim, cycloxydim, propoxydim, sethoxydim, tepraloxydim, tralkoxydim, and feproxydim.
• acetamide compounds include napropamide, dimethachlor, petoxamide, acetochlor, alachlor, allidochlor (CDAA), butenachlor, delacrol, diethylethyl, propisochlor, pirinachlor, butachlor, dimethenamid, dimethenamid P, metazachlor, metolachlor, S-metolachlor, pretilachlor, propachlor, thenylchlor, flufenacet, and mefenacet.
• bipyridylium compounds examples include cyperquat, morphamquat, diquat, and paraquat.
• allyloxyphenoxypropionic acid compounds include clodinafop, clodinafop propargyl, clofop, cyhalofop butyl, diclofop, diclofop methyl, diclofop P methyl, fenoxaprop, fenoxaprop ethyl, fenoxaprop P ethyl, fluazifop, fluazifop butyl, fluazifop P butyl, haloxyfop, haloxyfop methyl, haloxyfop P methyl, isoxapyripop, metamifop, propaquizafop, quizalofop, quizalofop ethyl, quizalofop P ethyl and quizalofop P tefuryl.
• carbamate compounds examples include asuram, carbetamide, desmedipham, chlorprocarb, phenisopham, cycloate, dimepiperate, pebulate, thiocarbazil, vernalate, barban, chlorbufam, chlorpropham, propham, swep, phenmedipham, butyrate, EPTC, esprocarb, molinate, orbencarb, prosulfocarb, pyributicarb, thiobencarb (benthiocarb), and triallate.
• pyridine compounds include aminopyralid, clopyralid, diflufenican, dithiopyr, fluridone, fluroxypyr, haloxifene, florpyrauxifene, picloram and its salts, picolinafen, thiazopyr, and triclopyr and its salts.
• Examples of the above urea compounds include benzthiazolone, bromuron, buturon, chlorbromuron, chloroxuron, difenoxuron, dimefuron, etidimuron, fenuron, fluothiuron, metobenzuron, metobromuron, metoxuron, monolinuron, monuron (CMU), nebulon, parafluron, siduron, thiazafluron, chlorotoluron, dymuron, diuron (DCMU), fluometuron, isoproturon, linuron, methabenzthiazuron, tebuthiuron, cumyluron, carbutilate, and isouron.
• the above dinitroaniline compounds include benfluralin (beslosin), butralin, dinitramine, ethalfluralin, fluchloralin, isopropaline, nitralin, profluralin, oryzalin, pendimethalin, prodiamine, and trifluralin.
• protoporphyrinogen oxidase (PPO) inhibitor compounds include acifluorfen, aclonifen, azafenidin, bifenox, chlomethoxynil, ethoxyfene, ethoxyfene ethyl, fomesafen, fluazolate, fluoroglycofen, fluoroglycofen ethyl, halosafen, lactofen, oxyfluorfen, butafenacil, epirifenacil, chlornitrofen (CNP), fluorodifen, fluoronitrofen (CFNP), nitrofen (NIP), oxyflufen, chlorphthalim, flumipropine, carfentrazone, carfentrazone ethyl, cinidon ethyl, flumiclorac pentyl, flumioxazin, fluthiacet, fluthiacet methyl, oxadiar
• the above triazine compounds include atraton, aziprothrin, chlorazine, cyprazine, desmetryn, dipropetryn, eglinadine ethyl, ipazine, metoprothrin, procyazine, progliadine, prometon, propazine, sebutylazine, secbumeton, terbumeton, trietazine, ametryn, atrazine, cyanazine, dimethamethryn, hexazinone, indaziflam, metamitron, metribuzin, prometryn, simazine (CAT), simetryn, terbuthylazine, terbutryn, and triaziflam.
• CAT simazine
• chlorfenprop dichlobenil (DBN), chlorthiamid (DCBN), cinmethylin, methiozolin, amitrole, flampro M, fosamine, methyldymron, monalid, MSMA, difenzoquat, diflufenzopyr, endothal and its salts, ethofumesate, etobenzanide, fenoxasulfone, fentrazamide, flupoxam, fluorochloridone, flurtamone, indanofan, tridiphane, ioxynil, ipfencarbazone, isoxaben, triazifuran, lenacil, methylarsonic acid, naptalam, flurochloridone, norflu Lazone, oxaziclomefone, pinoxaden, chloranocryl-dicryl, pentanochlor (CMMP), propanil, propyzamide, pyridate, pyroxas
• preparations preferably contain the above active ingredient in an amount of 0.01% to 90% by weight, and more preferably 0.1% to 50% by weight, based on the total weight of the preparation.
• the above preparation may be used as a treatment agent as it is, or may be used as a treatment agent prepared by mixing, etc. with the above-mentioned auxiliary agents or other active ingredients.
• the treatment agent may be prepared by mixing, etc., a preparation containing the other active ingredients with a preparation containing the above-mentioned active ingredients.
• the propagules treated with the treatment agent contain the active ingredient on its surface or inside. It is preferable that the propagules contain the active ingredient inside. It is preferable that the active ingredient is attached to the surface of the propagules, and more preferable that the active ingredient coats the surface of the propagules together with other ingredients added to the treatment agent as necessary.
• the amount of active ingredient contained in the propagules is preferably 0.05 ⁇ g to 100 mg per gram of propagules, more preferably 0.5 ⁇ g to 30 mg, even more preferably 0.5 ⁇ g to 8 mg, and particularly preferably 0.5 ⁇ g to 5 mg.
• the treated propagules can be grown and germinated under normal conditions for growing the propagules.
• the propagules can be stimulated with water, sunlight, nutrients, etc. to germinate.
• Growing can be done by sowing or burying the propagules in soil, or by placing the propagules in a liquid, solid, gel, or other medium.
• the propagules are treated with the active ingredient before growing begins (i.e., before sowing, etc.).
• Plants grown in this way can be grown to completion without being transplanted, or the seedlings that have grown to a certain extent can be transplanted into other soil or medium for further growth.
• the plant height of the broccoli plants in Treatment Example 1 and Treatment Example 2 was measured 35 days after sowing. The evaluation results are shown in Table 1.
• the seed treatment agents of Examples 1, 2 and 3 had a higher plant height and a better plant growth promotion effect than a seed treatment agent not containing EGT (Comparative Example 1) and broccoli treated with EGT by irrigation at the base of the plant. Furthermore, the seed treatment agents of Examples 1, 2 and 3 were found to have a better plant growth promotion effect, despite the amount of EGT treated per plant being significantly smaller than that of irrigation at the base of the plant (Comparative Example 2).
• the seed treatment agents of Examples 4, 5 and 6 had higher seed yields and better plant growth promotion effects than a seed treatment agent not containing EGT (Comparative Example 3) and Arabidopsis plants treated with EGT by bottom watering. Furthermore, the seed treatment agents of Examples 4, 5 and 6 were found to have better plant growth effects, despite the amount of EGT applied per plant being significantly smaller than that of bottom watering (Comparative Example 4).
• 0.1 g of tomato (Regina) seeds were placed in a microtube, 50 ⁇ L of seed treatment agent was added, the mixture was mixed by inversion, and then the mixture was air-dried.
• 1 kg of seedling soil (Takii Seeds) was placed in plastic pots measuring 135 mm in diameter and 114 mm in height, and one treated tomato seed was sown per pot. Five pots were placed in each treatment area, and the seeds were kept in a greenhouse with the room temperature set at 25°C.
• nursery soil (Takii Seeds) was placed in plastic pots measuring 135 mm in diameter and 114 mm in height, and one untreated tomato seed was sown per pot. Five pots were placed in each treatment area. The plants were kept in a greenhouse with a room temperature set at 25°C.
• Comparative Example 6 50 mL of EGT solution was added to the base of the tomato plants six times in total: 33rd, 35th, 37th, 39th, 41st, and 43rd days after sowing.
• the seed treatment agents of Examples 7, 8 and 9 produced more flower buds, more fruits and more fruit yields, and had superior growth-promoting effects, compared to a seed treatment agent not containing EGT (Comparative Example 5) and Arabidopsis plants treated with EGT by irrigation at the base of the plant. Furthermore, the seed treatment agents of Examples 7, 8 and 9 were found to have superior growth-promoting effects, despite the amount of EGT applied per plant being significantly smaller than that of irrigation at the base of the plant (Comparative Example 6).
• Mizuna (Senjiku Kyona) seeds were placed in a microtube, 20 ⁇ L of seed treatment agent was added, and the mixture was mixed by inversion and then air-dried. 5 mL of pure water was added to a 9 cm petri dish lined with filter paper, and 20 treated Mizuna seeds were sown.
• the temperature inside the artificial weather chamber was set to room temperature of 22°C, with a light period of 16 hours and a dark period of 8 hours. Light conditions were set so that the light intensity was 5000 lx in the center under fluorescent light irradiation.
• the seed treatment agents of Examples 10 and 11 had a higher germination rate and a superior germination promotion effect compared to Mizuna treated with EGT by hydroponic cultivation. Furthermore, the seed treatment agents of Examples 10 and 11 were confirmed to have an excellent germination promotion effect, even though the amount of EGT treated per plant was significantly smaller than that of hydroponic cultivation (Comparative Examples 7 and 8).
• 0.1 g of komatsuna seeds were placed in a microtube, 20 ⁇ L of seed treatment agent was added, the mixture was mixed by inversion, and then air-dried. 5 mL of pure water was added to a 9 cm petri dish lined with filter paper, and 20 treated komatsuna seeds were sown.
• the temperature inside the artificial weather chamber was set to room temperature of 22°C, with a light period of 16 hours and a dark period of 8 hours. Light conditions were set so that the light intensity was 5000 lx in the center under fluorescent light irradiation.
• EGT solution 10 mL was added to a 9 cm petri dish lined with filter paper, and 20 untreated Komatsuna seeds were sown.
• the temperature inside the artificial weather chamber was set to room temperature of 22°C, with a light period of 16 hours and a dark period of 8 hours. Light conditions were set so that the light intensity was 5000 lx in the center under fluorescent light irradiation.
• the seed treatment agents of Examples 12 and 13 produced taller plants and had superior plant growth promotion effects compared to a seed treatment agent not containing EGT (Comparative Example 9) and komatsuna plants treated with EGT by hydroponic cultivation. Furthermore, the seed treatment agents of Examples 12 and 13 were found to have superior plant growth effects, despite the amount of EGT treated per plant being significantly smaller than that of hydroponic cultivation (Comparative Examples 10 and 11).
• Example 6 Comparison of EGT content in Mizuna seeds
• the seed treatment agent of Example 14 was prepared so that the treatment amount of ergothioneine (EGT) was the EGT treatment amount shown in the following Table 6.
• EGT ergothioneine
• a commercially available EGT was used, and pure water was used as the solvent.
• Mizuna (Senjiku Kyona) seeds were placed in a microtube, 20 ⁇ L of seed treatment agent was added, and the mixture was mixed by inversion and then air-dried. 5 mL of pure water was added to a 9 cm petri dish lined with filter paper, and 20 treated Mizuna seeds were sown.
• the temperature inside the artificial weather chamber was set to room temperature of 22°C, with a light period of 16 hours and a dark period of 8 hours. Light conditions were set so that the light intensity was 5000 lx in the center under fluorescent light irradiation.
• EGT solution 10 mL was added to a 9 cm petri dish lined with filter paper, and 20 untreated shepherd's purse seeds were sown.
• the temperature inside the artificial climate chamber was set to room temperature of 22°C, with a light period of 16 hours and a dark period of 8 hours.
• the light conditions were set so that the light intensity was 5000 lx in the center under fluorescent light irradiation.
• Seeds from Treatment Examples 11 and 12 were collected 2 hours after sowing. The collected seeds were washed with pure water and then crushed in a mortar. The crushed seeds were suspended in 0.5 mL of pure water, and the suspension was heated at 120°C for 10 minutes. After cooling to room temperature, the seeds were centrifuged at 12,000 rpm for 5 minutes, and the EGT concentration of the collected supernatant was measured. The evaluation results are shown in Table 6.
• a method for treating plants is provided that can easily increase production.

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Environmental Sciences (AREA)
• Engineering & Computer Science (AREA)
• Wood Science & Technology (AREA)
• Pest Control & Pesticides (AREA)
• Zoology (AREA)
• Plant Pathology (AREA)
• Health & Medical Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Dentistry (AREA)
• Agronomy & Crop Science (AREA)
• Botany (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Soil Sciences (AREA)
• Chemical & Material Sciences (AREA)
• General Chemical & Material Sciences (AREA)
• Toxicology (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)
• Biodiversity & Conservation Biology (AREA)
• Ecology (AREA)
• Forests & Forestry (AREA)

Priority Applications (4)

Applications Claiming Priority (2)

Publications (1)

Family

ID=95448443

Family Applications (1)

Country Status (6)

Citations (16)

• 2024
  • 2024-10-10 AR ARP240102765A patent/AR134099A1/es unknown
  • 2024-10-15 JP JP2025553184A patent/JPWO2025084271A1/ja active Pending
  • 2024-10-15 KR KR1020267007383A patent/KR20260049295A/ko active Pending
  • 2024-10-15 WO PCT/JP2024/036625 patent/WO2025084271A1/ja active Pending
  • 2024-10-15 AU AU2024363433A patent/AU2024363433A1/en active Pending
  • 2024-10-15 CN CN202480056837.XA patent/CN121816114A/zh active Pending

Patent Citations (16)

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events