WO2024150580A1 - カラムカートリッジ - Google Patents

カラムカートリッジ Download PDF

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Publication number
WO2024150580A1
WO2024150580A1 PCT/JP2023/044336 JP2023044336W WO2024150580A1 WO 2024150580 A1 WO2024150580 A1 WO 2024150580A1 JP 2023044336 W JP2023044336 W JP 2023044336W WO 2024150580 A1 WO2024150580 A1 WO 2024150580A1
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WO
WIPO (PCT)
Prior art keywords
shape
column
flow path
cross
analytical column
Prior art date
Application number
PCT/JP2023/044336
Other languages
English (en)
French (fr)
Japanese (ja)
Inventor
祐輔 清水
雄一郎 橋本
祥人 渡部
一真 関谷
夢生 飯島
Original Assignee
株式会社日立ハイテク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社日立ハイテク filed Critical 株式会社日立ハイテク
Priority to CN202380086741.3A priority Critical patent/CN120380336A/zh
Priority to JP2024570092A priority patent/JPWO2024150580A1/ja
Publication of WO2024150580A1 publication Critical patent/WO2024150580A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column

Definitions

  • the present invention relates to a column cartridge that holds an analytical column in an analytical device that analyzes samples.
  • Liquid chromatography is an analytical method that uses an analytical column.
  • An analytical column is a cylindrical, elongated container packed under high pressure with a base material such as silica gel or polymer gel, and a filler of particles with various functional groups bonded to them.
  • Liquid chromatographs use a liquid as the mobile phase.
  • the temperature of the mobile phase in the analytical column increases, the viscosity of the mobile phase decreases, and the pressure decreases. For this reason, it is necessary to hold the analytical column of a liquid chromatograph in a column oven and adjust the temperature of the column.
  • Patent Document 1 discloses a column cartridge that houses an analytical column, a heater, and a temperature sensor.
  • the present invention provides a column cartridge for an analytical device that effectively prevents an analytical column from being installed in the wrong direction, facilitating correct analysis of samples.
  • the column cartridge of the analytical device of the present invention is a column cartridge that houses an analytical column of a liquid chromatograph, and the analytical column has a first flow path port having a first shape in a cross section perpendicular to the flow path, and a second flow path port having a second shape in the cross section that is different from the first shape.
  • the column cartridge is characterized by having a first engagement part having a shape that matches the first shape, and a second engagement part having a shape that matches the second shape.
  • the present invention effectively prevents the analytical column from being installed in the wrong direction, facilitating correct analysis of samples.
  • FIG. 1 is an external perspective view illustrating a structure of a column cartridge 1 of an analyzer (liquid chromatograph) according to a first embodiment.
  • FIG. FIG. 2 is a perspective view showing the structure of the upper surface of the column cartridge 1.
  • FIG. 2 is a perspective view showing the structure of the lower surface of the column cartridge 1.
  • 1A to 1C are a front view, left and right side views, etc., for explaining the configuration of the column cartridge 1 in more detail.
  • FIG. 2 is a perspective view illustrating the structure of the column cartridge 1.
  • FIG. 2 is a cross-sectional view illustrating the structure of the column cartridge 1.
  • FIG. 2 is a cross-sectional view illustrating the structure of the column cartridge 1.
  • FIG. 2 is a perspective view illustrating the structure of the column cartridge 1.
  • FIG. 10 is a cross-sectional view illustrating the structure of a column cartridge 1 of an analyzer (liquid chromatograph) according to a second embodiment.
  • FIG. 13 is a cross-sectional view illustrating the structure of a column cartridge 1 of an analyzer (liquid chromatograph) according to a third embodiment.
  • FIG. 10 is a cross-sectional view illustrating the structure of a column cartridge 1 of an analyzer (liquid chromatograph) according to a second embodiment.
  • FIG. 13 is a cross-sectional view illustrating the structure of a column cartridge 1 of an analyzer (liquid chromatograph) according to a third embodiment.
  • the column cartridge 1 includes a resin upper housing part 2 and a resin lower housing part 3.
  • the upper housing part 2 and the lower housing part 3 form a housing H that houses an analytical column 10 therein. That is, the upper housing part 2 is provided above the analytical column 10, while the lower housing part 3 is provided below the analytical column 10, and the analytical column 10 is housed inside the housing H that is formed by the upper housing part 2 and the lower housing part 3.
  • the analytical column 10 is fixed in the housing H by a metal block MB.
  • a handle portion (protrusion) 5 is formed on the top surface of the upper housing portion 2.
  • a first flow path port 4 is formed on the side of the housing between the upper housing portion 2 and the lower housing portion 3 as one end of the analytical column 10, and the analytical column 10 is held within the housing H in such a manner that the first flow path port 4 is exposed from the housing H.
  • the mobile phase flows into this first flow path port 4.
  • a second flow path port 9 is formed on the opposite side of the housing H as the other end of the analytical column 10, and the analytical column 10 is held within the housing H such that the second flow path port 9 is similarly exposed to the outside of the housing H.
  • Temperature detection windows 6A, 6B, 6C, 6D, and 6E are provided on the top surface of the upper housing part 2. These windows 6A to 6E are connected to the storage space of the analytical column 10.
  • the windows 6A, 6B, 6C, 6D, and 6E are arranged along the longitudinal direction of the analytical column 10. In Figure 2, some of the windows 6B, 6C, and 6D are formed at the position where the handle part 5 is formed, and the other windows 6A and 6E are arranged outside the handle part 5 (on the longitudinal extension line), but this is just an example and is not limited to what is shown.
  • the diameters of the windows 6A, 6B, 6C, 6D, and 6E are preferably about 1.4 mm or less.
  • Figure 3 shows the structure of the bottom surface of the column cartridge 1.
  • An opening 3A is formed in the bottom surface of the lower housing part 3.
  • a heat transfer part 7A of the lower metal block part 7 (described later) is inserted into this opening 3A, and the heat transfer part 7A is exposed to the outside from the opening 3A.
  • the heat transfer part 7A comes into contact with a heat source (not shown) and transfers heat to the analytical column 10.
  • Figures 4(a)-(c) are left side view, front view, and right side view of the upper housing 2.
  • Figures 4(d)-(e) are perspective view and front view of the analytical column 10.
  • Figures 4(f)-(h) are left side view, front view, and right side view of the lower housing 3. Note that the metal block MB is not shown in Figure 4.
  • the housing upper part 2 is provided above the analytical column 10, and upper engagement parts 21 and 22 with which both ends of the analytical column 10 engage are provided on both side parts (FIGS. 4(a) and 4(c)).
  • the upper engagement parts 21 and 22 are configured as cutout parts with which both ends of the analytical column 10 come into contact.
  • the upper engagement part 21 is a roughly rectangular cutout, and the upper engagement part 22 is a cutout with an arc-shaped upper edge.
  • the analytical column 10 has a flow path through which liquid such as a mobile phase moves, and has a first flow path port 4 and a second flow path port 9 at both ends of the flow path.
  • the liquid such as the mobile phase flows into the analytical column 10 from the first flow path port 4, which is the inlet of the flow path, and flows out from the second flow path port 9, which is the outlet of the flow path.
  • the first flow path port 4 and the second flow path port 9 of the analytical column 10 have different shapes, as described below.
  • the first flow path port 4 has a hexagonal cross section perpendicular to the direction of the flow path.
  • the second flow path port 9 also has a base 9B that has a hexagonal shape, but at its tip it has an irregularly shaped portion 9R that is a different shape from the hexagonal nut. Whether or not it is a "different shape” is determined in relation to the shapes of the engagement parts 21, 22, 31, and 32. In other words, a shape that prevents the engagement parts 21 and 31 from properly engaging with the irregularly shaped portion 9R when the analytical column 10 is not installed in the correct orientation is understood to be a "different shape" as used here.
  • the shape of the irregularly shaped portion 9R can be a shape that is approximately the same as the shape of the upper engagement portion 22 described above.
  • the shape of the irregularly shaped portion 9R can be a cylinder with a circular cross section and a diameter slightly smaller than the hexagonal base.
  • the shape (upper edge) of the lower engagement portion 22 can also be an arc shape that follows the cylindrical shape.
  • the analytical column 10 can be provided with a notch 10X to indicate its up-down direction.
  • the housing lower part 3 is provided below the analytical column 10, and on both side parts thereof, lower engagement parts 31 and 32 are provided with which both ends of the analytical column 10 come into contact ( Figures 4(f) and 4(g)).
  • the lower engagement parts 31 and 32 are configured as cutout parts with which both ends of the analytical column 10 come into contact.
  • the lower engagement part 31 is a substantially rectangular cutout part
  • the lower engagement part 32 is a circular arc cutout part.
  • the circular arc of the lower engagement part 32 has substantially the same shape as the irregular part 9R described above.
  • the upper engagement part 21 and the lower engagement part 31 together form a first engagement part that engages with the first flow path port 4, and the upper engagement part 22 and the lower engagement part 32 together form a second engagement part that engages with the irregular part 9R of the second flow path port 9.
  • the width of the lower engagement portion 31 is greater than the width of the first flow path opening 4, but like the upper engagement portion 21, it may be a cutout portion of approximately the same width as the first flow path opening 4.
  • Figure 5 is a perspective view of the column cartridge 1 with the metal block MB attached.
  • Figure 6 is a front view and left and right side views of the column cartridge 1.
  • Figure 7 is a cross-sectional view of the column cartridge 1 with the metal block MB attached.
  • An upper metal block 8 and a lower metal block 7 are disposed between the upper housing part 2 and the lower housing part 3.
  • the upper metal block 8 and the lower metal block 7 form a metal block MB.
  • the upper metal block 8 and the lower metal block 7 can be made of a metal with high thermal conductivity, such as aluminum.
  • the upper housing 2 and lower housing 3 are provided with a recess 2C and a protrusion 3C for fitting the two together.
  • the upper housing 2 is fitted into the lower housing 3 by fitting the protrusion 3C into the recess 2C, forming the housing H.
  • the lower housing 3 is also provided with a claw portion 3H that extends upward, and when the upper housing 2 is fitted into the lower housing 3, the claw portion 3H engages with the fitting groove 2H of the upper housing 2.
  • the upper metal block 8 is disposed opposite the upper housing 2.
  • the upper metal block 8 and the lower metal block 7 sandwich the analytical column 10 between them, and serve to fix the analytical column 10 in place within the column cartridge 1.
  • the upper metal block 8 has through holes 8A-8E directly below the windows 6A-6E (Figs. 6 and 8). As shown in Fig. 8, rod-shaped temperature sensor probes 15A, 15B, 15C, 15D, and 15E are inserted through the windows 6A-6E and through holes 8A-8E.
  • the diameter of the temperature sensor probes 15A, 15B, 15C, 15D, and 15E is approximately 0.7 mm.
  • the analytical column 10 needs to be attached in the correct orientation to the housing H, and if it is attached in the wrong orientation, correct analysis cannot be performed.
  • the first flow path port 4 and the second flow path port 9 at both ends of the analytical column 10 have different shapes, and the shapes of the engagement parts 21, 22, 31, and 32 correspond to these different shapes. Therefore, the operator can insert the analytical column 10 in the correct orientation by attaching the analytical column 10 so that the first flow path port 4 and the second flow path port 9 match the engagement parts 21, 22, 31, and 32.
  • the second flow passage port 9 has an irregularly shaped portion 9R. While attaching the analytical column 10 to the metal block MB, the operator can insert the analytical column 10 so that the irregularly shaped portion 9R aligns with the arcs of the upper engagement portion 22 and the lower engagement portion 32.
  • the irregular part 9R at the second flow passage port 9 does not match the shape of the engagement parts 21 and 22, while the first flow passage port 4 is too large compared to the engagement parts 31 and 32 and cannot be inserted, so the operator can notice the incorrect installation direction. Therefore, according to the first embodiment, it is possible to ensure that the analytical column 10 is reliably inserted in the correct direction.
  • the cross-sectional shape of the irregular part 9R is different from the cross-sectional shape of the first flow passage port 4, but it is preferable that the difference is such that the analytical column 10 cannot be attached in the opposite direction if it is attempted.
  • a column cartridge 1 according to a second embodiment will be described with reference to Fig. 9.
  • the column cartridge 1 of the second embodiment differs from the first embodiment in the structure of the analytical column 10 and the structure of the engagement portion.
  • the overall structure of the column cartridge 1 and other details are the same as those of the first embodiment (Figs. 1 to 3), so a duplicated description will be omitted.
  • FIG. 9 shows the cross-sectional structure of the first flow passage port 4, the second flow passage port 9, and the engagement portions 21, 22, 31, and 32 of the analytical column 10 in the column cartridge 1 in the second embodiment.
  • the first flow passage port 4 and the second flow passage port 9 in the first embodiment have a hexagonal cross-sectional structure, but in the second embodiment, the first flow passage port 4 and the second flow passage port 9 are so-called bale-shaped, with two opposing vertical sides being straight.
  • the analytical column 10 can be inserted so that the two parallel sides of the bale-shaped first flow passage port 4 match the vertical contours of the engagement portions 21 and 22.
  • the irregularly shaped portion 9R has a circular cross-section, as in the first embodiment.
  • the second embodiment can also achieve the same effects as the first embodiment.
  • a column cartridge 1 according to a third embodiment will be described with reference to Fig. 10.
  • the column cartridge 1 of the third embodiment differs from the first embodiment in the structure of the analytical column 10 and the structure of the engagement portion.
  • the overall structure of the column cartridge 1 and other details are the same as those of the first embodiment (Figs. 1 to 3), so a duplicated description will be omitted.
  • FIG. 10 shows the cross-sectional structure of the first flow path port 4, second flow path port 9, and engagement parts 21, 22, 31, and 32 of the analytical column 10 in the column cartridge 1 in the third embodiment.
  • the cross-sectional shape of the irregularly shaped part 9R is hexagonal rather than circular, and the upper engagement part 31 and lower engagement part 32 have a shape that matches the outline of this hexagon.
  • the same effects as in the first embodiment can be obtained.
  • the present invention is not limited to the above-described examples, and various modifications are possible.
  • the above-described examples have been described in detail to clearly explain the present invention, and the present invention is not necessarily limited to an embodiment that includes all of the configurations described. It is also possible to replace part of the configuration of one embodiment with the configuration of another embodiment. It is also possible to add the configuration of another embodiment to the configuration of one embodiment. It is also possible to delete part of the configuration of each embodiment, or to add or replace other configurations.
  • 1...column cartridge 2...upper housing part, 2C...recess, 2H...engagement groove, 3...lower housing part, 3A...opening, 3C...projection part, 3H...claw part, H...housing, 4...first flow path port, 5...handle part, 6A-6E...window part, 7...lower metal block part, 7A...heat transfer part, 8...upper metal block part, MB...metal block, 9...second flow path port, 9B...base part, 9R...irregularly shaped part, 10...analytical column, 15A-E...temperature sensor probe, 21, 22...upper engagement part, 31, 32...lower engagement part.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
PCT/JP2023/044336 2023-01-10 2023-12-12 カラムカートリッジ WO2024150580A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202380086741.3A CN120380336A (zh) 2023-01-10 2023-12-12 柱盒
JP2024570092A JPWO2024150580A1 (enrdf_load_stackoverflow) 2023-01-10 2023-12-12

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2023001509 2023-01-10
JP2023-001509 2023-01-10

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WO2024150580A1 true WO2024150580A1 (ja) 2024-07-18

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CN (1) CN120380336A (enrdf_load_stackoverflow)
WO (1) WO2024150580A1 (enrdf_load_stackoverflow)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012058515A2 (en) 2010-10-29 2012-05-03 Thermo Finnigan Llc Modular multiple-column chromatography cartridge
WO2017199335A1 (ja) * 2016-05-17 2017-11-23 株式会社日立ハイテクノロジーズ 分離カラム接続装置、接続方法及び分析システム
WO2020175651A1 (ja) * 2019-02-27 2020-09-03 株式会社日立ハイテク 分析装置のカラムオーブン
JP2022519969A (ja) * 2018-11-14 2022-03-28 アジレント・テクノロジーズ・インク 流体接続のための継手アセンブリ

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012058515A2 (en) 2010-10-29 2012-05-03 Thermo Finnigan Llc Modular multiple-column chromatography cartridge
WO2017199335A1 (ja) * 2016-05-17 2017-11-23 株式会社日立ハイテクノロジーズ 分離カラム接続装置、接続方法及び分析システム
JP2022519969A (ja) * 2018-11-14 2022-03-28 アジレント・テクノロジーズ・インク 流体接続のための継手アセンブリ
WO2020175651A1 (ja) * 2019-02-27 2020-09-03 株式会社日立ハイテク 分析装置のカラムオーブン

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CN120380336A (zh) 2025-07-25
JPWO2024150580A1 (enrdf_load_stackoverflow) 2024-07-18

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