WO2024110164A1 - Use of a composition for providing energizing glow on skin - Google Patents

Use of a composition for providing energizing glow on skin Download PDF

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Publication number
WO2024110164A1
WO2024110164A1 PCT/EP2023/080541 EP2023080541W WO2024110164A1 WO 2024110164 A1 WO2024110164 A1 WO 2024110164A1 EP 2023080541 W EP2023080541 W EP 2023080541W WO 2024110164 A1 WO2024110164 A1 WO 2024110164A1
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WO
WIPO (PCT)
Prior art keywords
composition
vitamin
skin
derivative
acid
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Application number
PCT/EP2023/080541
Other languages
French (fr)
Inventor
Mohini Anand Bapat
Anita DAMODARAN
Naresh Dhirajlal Ghatlia
Amitabha Majumdar
Gouri Gupchup MALHOTRA
Mruthyunjaya Swamy MATHAPATHI
Simone SETHNA
Satish Kumar VENKATESH
Original Assignee
Unilever Ip Holdings B.V.
Unilever Global Ip Limited
Conopco, Inc., D/B/A Unilever
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Application filed by Unilever Ip Holdings B.V., Unilever Global Ip Limited, Conopco, Inc., D/B/A Unilever filed Critical Unilever Ip Holdings B.V.
Publication of WO2024110164A1 publication Critical patent/WO2024110164A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/345—Alcohols containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/676—Ascorbic acid, i.e. vitamin C
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/678—Tocopherol, i.e. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations

Definitions

  • the present invention relates to use of a composition that provides energizing glow to skin. It more particularly relates to a topical application that delivers a fresh, young and lively look to skin that has a glowing appearance.
  • Energizing brightness is another one of the benefits that some products in the market have been providing. Energizing brightness means that the skin gets a fresh and young look, with a lively appearance, which recharges the skin and helps protect against external and internal stresses.
  • actives available in the market that are claimed to deliver this benefit e.g. Oli’VineTM ST a product sold by Gattefosse. It has the INCI name Water (and) Olivine Extract. It has been shown that use of such actives increases ATP (adenosine triphosphate) levels and reduces intracellular oxidation which are the key processes that deliver the claimed benefits to the skin.
  • the present inventors in wishing to deliver such a benefit with known and commonly available ingredients that are safe and are inexpensive, have hit upon a unique combination of vitamins at very low concentrations which surprisingly works even when incorporated in cleansing compositions. They have found that a combination of vitamin C and vitamin E when used at as low a concentration as less than 20 ppm in a wash off composition work synergistically to provide energizing glow to skin as evidenced by the resultant ATP levels. The present inventors are not aware of any published information which implicates either of these actives for providing enhanced ATP levels. Thus, it was indeed surprising to the inventors that a combination of two actives, neither of which is known for enhancing ATP levels are capable of delivering energizing glow to skin by interacting synergistically. It was further surprising that this could be achieved at incorporation of very low concentration of less than 20 ppm, that too in a wash-off composition.
  • LIS2018/325804 discloses skin care regimens which use five components that each include a composition made with a combination of a decolorized muscadine (Vitis rotundi folia) pomace solvent extract, beta-glucan and grape seed extract, to improve the appearance of skin.
  • the compositions may additionally include other ingredients that are present in a sufficient amount to improve the appearance of skin, such as panthenol, Vitamins A, C, and E, superoxide dismutase, schizandra chinensis extract, lotus japonicus extract, or any combination thereof.
  • compositions and methods for their use for cosmetic applications can include tetrahexyldecyl ascorbate, Phyllanthus emblica fruit extract, and tocopherol, ascorbic acid.
  • the composition can further include vegetable amino acids.
  • An effective amount of the composition can be applied on skin to stimulate collagen production and lysyl oxidase activity, inhibit tyrosinase, inhibit melanogenesis and/or COX-1 activity, and inhibit the matrix metalloproteinase enzyme activity in skin.
  • EP2637636A1 (Unilever) provides leave-on non-solid skin conditioning compositions.
  • the composition comprises from about 0.01 to about 15% by weight of the composition of 12- hydroxystearic acid, from about 0.1 to about 30% by weight of the composition of a nonionic surfactant and from about 0.05% to about 10% of a thickening polymer.
  • W009138801A (NB LABS LIMITED) discloses skin anti-aging composition comprising a skin cellular synthesis booster, a skin cellular energiser and a skin cellular cleanser, together with a cosmetically-acceptable excipient.
  • the composition may be formulated as a two-part formulation.
  • the first aspect of the present invention relates to use of a composition comprising (i) vitamin C or its derivative and (ii) vitamin E or its derivative to increase adenosine triphosphate (ATP) levels in the skin as compared to the composition without vitamin C or its derivative and without vitamin E or its derivative.
  • vitamin C or its derivative is selected from the group comprising ascorbic acid, salts of ascorbic acid, oxidized and reduced forms of vitamin C, sodium ascorbyl phosphate, 3-0-Ethyl ascorbic acid, ascorbyl methylsilanol pectinate, magnesium ascorbyl phosphate, ascorbyl glucoside, tetrahexyldecyl ascorbate, aminopropyl ascorbyl phosphate, ascorbyl Palmitate, and mixtures thereof; and wherein vitamin E or its derivative selected from the group comprising a-,p-,y-, and o-tocopherols, their related corresponding tocotrienols and mixtures thereof
  • Another aspect of the present invention relates to a cleansing composition
  • a cleansing composition comprising
  • the first aspect of the present invention relates to use of a composition comprising (i) vitamin C or its derivative and (ii) vitamin E or its derivative for providing energizing glow to skin.
  • the use is preferably non-therapeutic. This benefit is achieved by inclusion of a very low concentration of these ingredients in the composition.
  • Vitamin C or its derivative is preferably included in 0.00001 to 0.002% by weight of the composition.
  • Vitamin E or its derivative is preferably included in 0.00001 to 0.002% by weight of the composition.
  • the composition comprises vitamin C or a derivative thereof.
  • Vitamin C ascorbic acid, ascorbate
  • Vitamin C is a simple low-molecular-weight carbohydrate that is essential for the body as a water-soluble vitamin.
  • As an antioxidant, vitamin C has both oxidized and reduced forms: L- dehydroascorbic and L-ascorbic acid.
  • Vitamin C is involved in the formation of the skin barrier and collagen in the dermis and plays a physiological role in the skin against skin oxidation, in antiaging of wrinkles, and in cell signal pathways of cell growth and differentiation, which are related to the occurrence and development of various skin diseases.
  • Vitamin C or its derivatives which may be used in the present invention may be one or more of the following:
  • Sodium ascorbyl phosphate is an active stable vitamin C derivative ingredient for the cosmetics industry. It liberates vitamin C in the skin and protects the cells of the skin, promotes collagen formation, controls the formation of senile keratosis, and lightens dark skin.
  • 3-O-ethyl ascorbic acid promotes the production of collagen, which significantly improves the structure of the skin cells and the overall condition of skin.
  • Magnesium ascorbyl phosphate has all the functions of vitamin C and is very stable. It has excellent anti-oxidation and protection benefits, and can effectively resist UV radiation and promotes collagen production. This product is recommended for skin whitening/lightening applications, as well as anti-aging and anti-wrinkle products.
  • Vitamin C L-ascorbic acid
  • USP is an active form of vitamin C as it occurs naturally. It is a potent antioxidant (shown to be able to protect skin from oxidative damages). It can improve appearance of aged and fragile skin. Widely used as add-on ingredient in skin-lightening products to correct hyperpigmentation and age spots.
  • Ascorbyl glucoside is a water-soluble Vitamin C derivative with superior stability. It resists degradation, and has the same lightening, sun protective and anti-aging properties as ascorbic acid.
  • Tetrahexyldecyl ascorbate is a very stable, oil-soluble Vitamin C ester but has no inherent capabilities as an antioxidant because all hydroxyl groups are esterified.
  • Vitamin C or its derivative is preferably included in 0.00001 to 0.002%, more preferably 0.0001 to 0.0015% by weight of the composition.
  • Tocopheryl Acetate or Alpha Tocopheryl Acetate is commonly known as Vitamin E. It has the structure as given below:
  • Alpha-tocopheryl acetate also known as tocopheryl acetate, is a synthetic form of vitamin E.
  • a- tocopheryl acetate is a fat-soluble vitamin E and considered the most stable and active form of vitamin E.
  • Alpha tocopheryl acetate is known for its antioxidant properties and it protects cells from oxidative stress induced damages.
  • Tocopheryl acetate can be found in many skincare products where it is used as a skinconditioning agent. Its antioxidant effect helps in preventing skin damages caused by excessive exposure to ultraviolet (UV) radiation emitted by the sun. This product is recommended for skin whitening/lightening applications, as well as anti-aging and anti-wrinkle products.
  • UV ultraviolet
  • D-Alpha tocopherol acetate has the structure as give below.
  • d-alpha-Tocopherol acetate is a closely related chemical with alpha-Tocopherol acetate
  • d- alpha-tocopherol is a natural active ingredient of Vitamin E, it also has antioxidant potency and reduces oxidative stress induced damages in cells.
  • D a-Tocopherol succinate is a fat-soluble vitamin and one of the most potent antioxidant tocopherol. Its antioxidant property helps in reducing oxidative stress induced damages in cells.
  • Vitamin E or its derivatives are available from BASF and Matrix Lifescience. BASF markets these materials under the names Copherol R 1250 and Vitamin E acetate Care.
  • Mitochondria is known as a powerhouse of the cell as this produces bulk of the cellular ATP. Exposure to external factors like UV, pollution etc., induces reactive oxygen species (ROS) in the cells leading to oxidative stress. The excessive reactive oxygen species causes cellular damage and impacts on mitochondrial functions, thus reduces ATP synthesis. In addition, the reactive oxygen species like superoxide, singlet oxygen, peroxides are also produced during the process of cellular respiration and metabolism under normal condition which could also have undesirable consequences if not quenched by antioxidants. Vitamin C is a water-soluble vitamin having strong antioxidant properties. Apart from direct antioxidant function of Vitamin C, it also enhances endogenous antioxidant enzymes in the cells to reduces oxidative stress induced damages.
  • Vitamin E by its direct antioxidant mechanism and also boosting cellular antioxidant system, negate the effect of oxidative stress induced damages which in turn improves the ATP level in the cell.
  • Vitamin E is fat-soluble antioxidant and used as an important ingredient in many cosmetic products. It protects the skin from various deleterious effects caused by UV exposure by acting as a free-radical scavenger. Many studies suggest that vitamin E has antitumorigenic and photoprotective properties. Since, Vitamin E is a fat-soluble antioxidant, it plays a major role in mitochondrial membrane, which reacts with the peroxyl radicals faster and protects membrane from excessive oxidative damage and thereby enhances mitochondrial function.
  • the composition additionally includes hydroxystearic acid.
  • hydroxy stearic acid is 10-hydroxystearic acid, 12-hydroxystearic acid or trihydoxystearic acid (e.g. 9,10,13-trihydroxystearic acid) or trihydroxy stearin or compounds that yield one or more molecules of hydroxy stearic acid or hydroxystearate on their breakdown like mono, di or tri ester of glycerol with hydroxy stearic acid.
  • 10-hydroxystearic acid, 12- hydroxystearic acid and 9,10,13-trihydroxystearic acid are more preferred, 12-hydroxystearic acid (12-HSA) being most preferred.
  • 12-HSA has the structure as given below:
  • Hydroxystearic acid is preferably included in 0.001 to 1 %, more preferably 0.01 to 1%, further more preferably 0.1 to 0.5% by weight of the composition.
  • the composition of the invention may include a humectant which is generally a polyhydric alcohol.
  • humectant is at least one of propylene glycol, dipropylene glycol, polypropylene glycol, polyethylene glycol, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1 ,3-butylene glycol, isoprene glycol, 1 ,3 propanediol, pentylene glycol, hexylene glycol, 1 ,2,6-hexanetriol, glycerol, ethoxylated glycerol, and propoxylated glycerol.
  • Preferred humectants are selected from one or more of glycerine, 1 ,3-butylene glycol, propylene glycol, 1 ,3 propanediol, pentylene glycol, hexylene glycol, and sorbitol.
  • Glycerol is the most preferred humectant. It is preferred that the amount of humectant is 0.01 to 10 %, more preferably 0.1 to 5% by weight of the composition.
  • the topical composition of the invention is meant to be used for personal care or for cosmetic use and could also be referred to as a personal care composition or a cosmetic composition.
  • a “personal care composition” as used herein is meant to include a composition for topical application i.e external surfaces of the skin and/or hair of humans. Such a composition may be classified as leave-on or wash off, and includes any product applied to a human body for improving appearance, cleansing, odour control or general aesthetics.
  • the composition is preferably a cleansing composition of the wash off type which comprises a surfactant.
  • the surfactant is preferably an anionic surfactant preferably soap.
  • the anionic surfactant is preferably included in 1 to 80% by weight of the composition.
  • “Skin” as used herein is meant to include skin on the face and body (e.g. neck, chest, back, arms, underarms, hands, legs and scalp) and especially to the exposed parts thereof.
  • composition of the invention could also be incorporated in a format for cleansing topical surfaces of the human or animal body.
  • Cleansing composition are of the so called wash-off or rinse-off format. By these formats, is meant that the composition is generally diluted with water and applied on the topical surface e.g. the skin or hair and then rinsed off with copious amounts of water after a few minutes.
  • Cleansing composition could be in any format either in solid form or in liquid form. When in solid form it is preferably a bar which may contain soap, synthetic anionic surfactant or a combination of the two.
  • the soap for preparing the cleansing composition of the invention is preferably a C8-C24 soap, more preferably C10-C20 soap and most preferably C12-C18 soap.
  • the cation of the soap can be alkali metal, alkaline earth metal or ammonium.
  • the cation of the soap is selected from sodium, potassium or ammonium. More preferably the cation of the soap is sodium or potassium.
  • Fatty acids derived from other suitable oils/fats such as groundnut, soybean, tallow, palm, palm kernel, etc. may also be used in other desired proportions.
  • Synthetic anionic surfactants for use in wash-off compositions are preferably selected from alkyl ether sulphate, primary alkyl sulphate, secondary alkyl sulphonates, alkyl benzene sulphonates, or ethoxylated alkyl sulphates.
  • the anionic surfactant other than soap which is preferred in the cleansing composition is an alkyl ether sulphate preferably those having between 1 and 3 ethylene oxide groups, either from natural or synthetic source and/or sulphonic acid. Especially preferred are sodium lauryl ether sulphates.
  • Alkyl polyglucoside may also be present in the composition, preferably those having a carbon chain length between Ce and C .
  • the anionic surfactant is preferably present in an amount of 1 to 80%, preferably from 10 to 85%, more preferably 25 to 75% by weight of the cleansing composition.
  • the cleansing composition is preferably in the form of a solid or semi solid form, most preferably in a solid form.
  • Preferred solid compositions are in the shape of a soap bar.
  • Preferred solid cleansing compositions may include other known ingredients such as perfumes, pigments, preservatives, emollients, sunscreens, gelling agents and thickening agents.
  • Water is a preferred carrier. When water is present, it is preferably present in at least 1%, more preferably at least 2%, further more preferably at least 5% by weight of the composition.
  • a preferred cleansing composition comprises 10 to 50%, more preferably 12 to 40%, most preferably 12 to 22% by weight water.
  • the cleansing composition of the invention may also be delivered through a moisturizing bar or a moisturizing liquid composition.
  • Moisturizing bar compositions comprising fatty acyl isethionates (e.g. cocyl isethionate) are especially preferred.
  • Fatty acyl isethionates e.g., cocoyl isethionates
  • surfactant "products" are defined as mixtures of anionic acyl isethionate surfactants and fatty acids/fatty acid soaps. They are highly desirable in personal care skin or hair cleansing products, particularly in personal care products, because they lather well, are mild to the skin and have good emollient properties.
  • fatty acid isethionate surfactant products are produced by esterification of fatty acids or by reaction of fatty acid chloride having carbon chain length of C8 to C20 with isethionate.
  • a typical surfactant product containing fatty acyl isethionate contains about 40 to 95 wt.% acid isethionate, and 5 to 50 wt.%, typically 10 to 40 wt.% free fatty acid, in addition to isethionate salts, typically at less than 5%, and trace (less than 2 wt.%) of other additives.
  • Fatty acid soap may be included in the range of 5 to 15 wt%.
  • Other surfactants like betaines may be included in 1 to 5 wt%. Water is generally included in 2 to 8 wt% of the composition.
  • Wash-off composition in the form of a liquid generally comprises surfactants at low concentration and are mild on skin.
  • the surfactant is generally included in 4 to 18%, preferably 6 to 12% by weight of the liquid cleansing composition.
  • Surfactants for inclusion in the liquid cleansing composition of the invention may preferably be of the anionic, non-ionic, cationic or amphoteric types.
  • a useful surfactant for inclusion in the liquid cleansing composition of the invention is sodium lauryl ether sulphate (SLES).
  • SLES for use in the present invention generally preferably has 1 to 3 Ethoxylate (EO) groups.
  • SLES is preferably included in 3 to 8% by weight of the composition.
  • the other surfactant which may be included in the present invention is cocoamide monoethanol amine (CMEA).
  • CMEA is preferably included in 1 to 3% by weight of the composition.
  • the surfactant comprises a mixture of sodium lauryl ether sulphate (SLES) and coco amide monoethanol amine (CMEA).
  • Another useful surfactant for inclusion in the liquid cleansing composition of the invention is an amphoteric surfactant preferably a betaine surfactant, more preferably an alkyl amidopropyl betaine surfactant for example cocamidopropyl betaine.
  • the composition comprises from 0.1 to 5 wt.%, preferably from 0.5 to 4 wt.%, more preferably from 1 to 3 wt.% of a betaine surfactant.
  • Surfactants of the non-ionic class for inclusion in the liquid cleansing composition of the invention are preferably of the polyoxyethylene sorbitan alkyl esters class (sold as Tween surfactants)), fatty alcohol ethoxylates (sold as Brij surfactants), alkyl phenol ethoxylates (sold as Triton surfactants), fatty acid ethoxylates (sold as Myrj surfactants), and Alkyl poly glucosides (sold as Plantacare surfactants).
  • Water is a preferred carrier in liquid cleansing compositions of the invention.
  • water is generally present in 70 to 95% by weight.
  • the composition may also additionally comprise a free fatty acid other than hydroxy stearic acid.
  • the free fatty acid is preferably included in 0.01 to 5% by weight of the composition.
  • the free fatty acid may be one or more of C8-C24, preferably C10-C20 and most preferably C12-C18 fatty acid.
  • the free fatty acids can exist as unsaponified fatty acids in the final product.
  • Preferred liquid cleansing compositions may include other known ingredients such as electrolytes, perfumes, pigments, preservatives, emollients, sunscreens, emulsifiers, gelling agents and thickening agents.
  • Another aspect of the present invention relates to a method of providing energizing glow to skin comprising the steps of (i) applying the cleansing composition of the invention on to said skin preferably diluted with water, followed by (ii) rinsing said skin with water.
  • the method is preferably cosmetic or non-therapeutic.
  • Yet another aspect of the present invention relates to use of a combination of vitamin C or its derivative and Vitamin E or its derivative to boost ATP levels in HDFa cells.
  • vitamin C or its derivative is selected from the group comprising ascorbic acid, salts of ascorbic acid, oxidized and reduced forms of vitamin C, sodium ascorbyl phosphate, 3-0-Ethyl ascorbic acid, ascorbyl methylsilanol pectinate, magnesium ascorbyl phosphate, ascorbyl glucoside, tetrahexyldecyl ascorbate, aminopropyl ascorbyl phosphate, ascorbyl Palmitate, and mixtures thereof; and wherein vitamin E or its derivative selected from the group comprising a-,p-,y-, and o- tocopherols, their related corresponding tocotrienols and mixtures thereof.
  • vitamin C or its derivative is included in 0.00001 to 0.002% by weight of the composition.
  • vitamin E or its derivative is included in 0.00001 to 0.002% by weight of the composition.
  • composition additionally comprises 0.001 to 1 wt% hydroxy stearic acid.
  • hydroxystearic acid is 12-hydroxystearic acid.
  • composition is a cleansing composition comprising a surfactant.
  • the surfactant comprises an anionic surfactant preferably soap.
  • anionic surfactant is included in 1 to 80 wt% of the composition.
  • composition comprises a humectant preferably glycerol.
  • the present invention provides a cleansing composition
  • a cleansing composition comprising
  • composition additionally comprises 0.001 to 1 wt% hydroxystearic acid.
  • composition additionally comprises 0.01 to 5 wt% free fatty acid other than said hydroxystearic acid.
  • the composition additionally comprises a humectant preferably glycerol.
  • the present invention provides a method increase adenosine triphosphate (ATP) levels in the skin comprising the steps of (i) applying a composition as claimed in any one of the preceding claims 10 to 13 on to said skin preferably diluted with water, followed by (ii) rinsing said skin with water.
  • ATP adenosine triphosphate
  • Examples A - F and 1-3 Interaction between Vitamin C and Vitamin E to deliver synergistic ATP levels
  • HDFa Human dermal fibroblast cells were seeded in 96 well plate and maintained in a 5% CO2 incubator at 37°C for 24 h. Then cells were treated with different concentrations of Vitamin C (VC) or Vitamin E (VE) or a combination of VC+VE with fresh media and incubation was continued for 1 h. Post 1 h of incubation, cells were lysed by adding 30 pl of RIPA buffer with protease and phosphatase inhibitor. The cell lysate was used for estimating ATP levels using ATP determination Kit. The ATP data is normalized with total protein from the respective treatments and the ATP levels were measured with and without treatment.
  • VC Vitamin C
  • VE Vitamin E
  • the ATP levels as measured are also shown in Table -1 .
  • Table -1 The data in Table - 1 above indicates that the combination of Vitamin C and Vitamin E at any concentration (from 0.01 to 1 ppm) is superior to that of using an individual active at the same concentration, thereby indicating synergistic activity.
  • a cleaning composition as given in Table -2 below was found to be useful for delivering the energizing glow by way of the present invention.

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Abstract

The present invention relates to use of a composition that provides energizing glow to skin. It more particularly relates to a topical application that delivers a fresh, young and lively look to skin that has a glowing appearance. This has been achieved by use of a composition that comprises a combination of vitamin C and Vitamin E. This has been found effective even when delivered through a cleansing composition.

Description

USE OF A COMPOSITION FOR PROVIDING ENERGIZING GLOW ON SKIN
Field of the Invention
The present invention relates to use of a composition that provides energizing glow to skin. It more particularly relates to a topical application that delivers a fresh, young and lively look to skin that has a glowing appearance.
Background of the Invention
People in general like to look good and thereby feel good about themselves. In addition to eating good food and living a healthy lifestyle that includes breathing clean air and having balanced amount of exercise, they also rely on cosmetic products to keep their external appearance as close to their perception of what good looks constitute. Skin is the largest organ in the human body and the one that is most visible to the external world. In skin, most consumers want to have an even skin tone that is free of blotches, hyperpigmentation, wrinkles and visible signs of acne. Very many cosmetic products are available to deliver one or more of the above benefits.
Many of the above problems are exacerbated by natural aging and internal and external stresses like light, excessive sunshine, pollution, hormonal changes and mental stress. The present inventors have worked on providing solutions to some of the above problems and developed technologies that deliver even skin tone, skin brightness and products that mask visible skins of aging like wrinkles.
Energizing brightness is another one of the benefits that some products in the market have been providing. Energizing brightness means that the skin gets a fresh and young look, with a lively appearance, which recharges the skin and helps protect against external and internal stresses. There are actives available in the market that are claimed to deliver this benefit e.g. Oli’Vine™ ST a product sold by Gattefosse. It has the INCI name Water (and) Olivine Extract. It has been shown that use of such actives increases ATP (adenosine triphosphate) levels and reduces intracellular oxidation which are the key processes that deliver the claimed benefits to the skin.
The present inventors in wishing to deliver such a benefit with known and commonly available ingredients that are safe and are inexpensive, have hit upon a unique combination of vitamins at very low concentrations which surprisingly works even when incorporated in cleansing compositions. They have found that a combination of vitamin C and vitamin E when used at as low a concentration as less than 20 ppm in a wash off composition work synergistically to provide energizing glow to skin as evidenced by the resultant ATP levels. The present inventors are not aware of any published information which implicates either of these actives for providing enhanced ATP levels. Thus, it was indeed surprising to the inventors that a combination of two actives, neither of which is known for enhancing ATP levels are capable of delivering energizing glow to skin by interacting synergistically. It was further surprising that this could be achieved at incorporation of very low concentration of less than 20 ppm, that too in a wash-off composition.
Database GNPD (online); Mintel; 6 January 2021 , “15Vitamin C+ Hyaluronic acid”, XP093011390, Database accession number-8382495. Database GNPD (online); Mintel; 21 August 2022, “Clarifying facial cleanser”, XP093041221 , Database accession number-9832656. These references from Mintel mention some combinations of vitamin C and vitamin E
LIS2018/325804 discloses skin care regimens which use five components that each include a composition made with a combination of a decolorized muscadine (Vitis rotundi folia) pomace solvent extract, beta-glucan and grape seed extract, to improve the appearance of skin. The compositions may additionally include other ingredients that are present in a sufficient amount to improve the appearance of skin, such as panthenol, Vitamins A, C, and E, superoxide dismutase, schizandra chinensis extract, lotus japonicus extract, or any combination thereof.
US2022218594 (CARLE TIFFANY Et al.) discloses compositions and methods for their use for cosmetic applications. The composition can include tetrahexyldecyl ascorbate, Phyllanthus emblica fruit extract, and tocopherol, ascorbic acid. The composition can further include vegetable amino acids.. An effective amount of the composition can be applied on skin to stimulate collagen production and lysyl oxidase activity, inhibit tyrosinase, inhibit melanogenesis and/or COX-1 activity, and inhibit the matrix metalloproteinase enzyme activity in skin.
EP2637636A1 (Unilever) provides leave-on non-solid skin conditioning compositions. The composition comprises from about 0.01 to about 15% by weight of the composition of 12- hydroxystearic acid, from about 0.1 to about 30% by weight of the composition of a nonionic surfactant and from about 0.05% to about 10% of a thickening polymer. W009138801A (NB LABS LIMITED) discloses skin anti-aging composition comprising a skin cellular synthesis booster, a skin cellular energiser and a skin cellular cleanser, together with a cosmetically-acceptable excipient. The composition may be formulated as a two-part formulation.
It is thus an object of the present invention to increase ATP levels in the skin and deliver energizing glow to skin.
It is another object of the present invention to deliver the above benefit from a wash-off composition.
Summary of the Invention
The first aspect of the present invention relates to use of a composition comprising (i) vitamin C or its derivative and (ii) vitamin E or its derivative to increase adenosine triphosphate (ATP) levels in the skin as compared to the composition without vitamin C or its derivative and without vitamin E or its derivative.; wherein vitamin C or its derivative is selected from the group comprising ascorbic acid, salts of ascorbic acid, oxidized and reduced forms of vitamin C, sodium ascorbyl phosphate, 3-0-Ethyl ascorbic acid, ascorbyl methylsilanol pectinate, magnesium ascorbyl phosphate, ascorbyl glucoside, tetrahexyldecyl ascorbate, aminopropyl ascorbyl phosphate, ascorbyl Palmitate, and mixtures thereof; and wherein vitamin E or its derivative selected from the group comprising a-,p-,y-, and o-tocopherols, their related corresponding tocotrienols and mixtures thereof.
Another aspect of the present invention relates to a cleansing composition comprising
(i) 0.00001 to 0.002 wt% vitamin C or its derivative;
(ii) 0.00001 to 0.002 wt% vitamin E or its derivative;
(iii) 1 to 80 wt% anionic surfactant; and
(iv) 10 to 90 wt% water. Detailed description of the Invention
These and other aspects, features and advantages will become apparent to those of ordinary skill in the art from a reading of the following detailed description and the appended claims. For the avoidance of doubt, any feature of one aspect of the present invention may be utilised in any other aspect of the invention. The word "comprising" is intended to mean "including" but not necessarily "consisting of' or "composed of." In other words, the listed steps or options need not be exhaustive. It is noted that the examples given in the description below are intended to clarify the invention and are not intended to limit the invention to those examples per se. Similarly, all percentages are weight/weight percentages unless otherwise indicated. Except in the operating and comparative examples, or where otherwise explicitly indicated, all numbers in this description and claims indicating amounts of material or conditions of reaction, physical properties of materials and/or use are to be understood as modified by the word "about". Numerical ranges expressed in the format "from x to y" are understood to include x and y. When for a specific feature multiple preferred ranges are described in the format "from x to y", it is understood that all ranges combining the different endpoints are also contemplated.
The first aspect of the present invention relates to use of a composition comprising (i) vitamin C or its derivative and (ii) vitamin E or its derivative for providing energizing glow to skin. The use is preferably non-therapeutic. This benefit is achieved by inclusion of a very low concentration of these ingredients in the composition. Vitamin C or its derivative is preferably included in 0.00001 to 0.002% by weight of the composition. Vitamin E or its derivative is preferably included in 0.00001 to 0.002% by weight of the composition.
The composition comprises vitamin C or a derivative thereof. Vitamin C (ascorbic acid, ascorbate) is a simple low-molecular-weight carbohydrate that is essential for the body as a water-soluble vitamin. As an antioxidant, vitamin C has both oxidized and reduced forms: L- dehydroascorbic and L-ascorbic acid.
Vitamin C is involved in the formation of the skin barrier and collagen in the dermis and plays a physiological role in the skin against skin oxidation, in antiaging of wrinkles, and in cell signal pathways of cell growth and differentiation, which are related to the occurrence and development of various skin diseases. Vitamin C or its derivatives which may be used in the present invention may be one or more of the following:
Sodium ascorbyl phosphate:
Sodium ascorbyl phosphate is an active stable vitamin C derivative ingredient for the cosmetics industry. It liberates vitamin C in the skin and protects the cells of the skin, promotes collagen formation, controls the formation of senile keratosis, and lightens dark skin.
3-0-Ethyl ascorbic acid:
3-O-ethyl ascorbic acid promotes the production of collagen, which significantly improves the structure of the skin cells and the overall condition of skin.
Ascorbyl methylsilanol pectinate:
It is a known antioxidant and is known to improve skin texture, as well as helping with reducing aging and dark spots.
Magnesium ascorbyl phosphate (MAP):
Magnesium ascorbyl phosphate (MAP) has all the functions of vitamin C and is very stable. It has excellent anti-oxidation and protection benefits, and can effectively resist UV radiation and promotes collagen production. This product is recommended for skin whitening/lightening applications, as well as anti-aging and anti-wrinkle products.
Ascorbic acid:
Vitamin C (L-ascorbic acid), USP is an active form of vitamin C as it occurs naturally. It is a potent antioxidant (shown to be able to protect skin from oxidative damages). It can improve appearance of aged and fragile skin. Widely used as add-on ingredient in skin-lightening products to correct hyperpigmentation and age spots.
Figure imgf000007_0001
Ascorbyl glucoside:
Ascorbyl glucoside is a water-soluble Vitamin C derivative with superior stability. It resists degradation, and has the same lightening, sun protective and anti-aging properties as ascorbic acid.
Tetrahexyldecyl ascorbate:
Tetrahexyldecyl ascorbate is a very stable, oil-soluble Vitamin C ester but has no inherent capabilities as an antioxidant because all hydroxyl groups are esterified.
It has excellent skin penetration and as a result offers increased cell protection against IIV-B radiation. It can improve appearance of aged and fragile skin. It is widely used as an add-on ingredient in skin-lightening products to correct hyperpigmentation and age spots.
Aminopropyl ascorbyl phosphate:
It is a stabilized vitamin C derivative. It is ideal for whitening, anti-wrinkle, and anti-oxidation applications.
Vitamin C or its derivative is preferably included in 0.00001 to 0.002%, more preferably 0.0001 to 0.0015% by weight of the composition.
Tocopheryl Acetate or Alpha Tocopheryl Acetate is commonly known as Vitamin E. It has the structure as given below:
Figure imgf000008_0001
Alpha-tocopheryl acetate , also known as tocopheryl acetate, is a synthetic form of vitamin E. a- tocopheryl acetate is a fat-soluble vitamin E and considered the most stable and active form of vitamin E. Alpha tocopheryl acetate is known for its antioxidant properties and it protects cells from oxidative stress induced damages.
Tocopheryl acetate can be found in many skincare products where it is used as a skinconditioning agent. Its antioxidant effect helps in preventing skin damages caused by excessive exposure to ultraviolet (UV) radiation emitted by the sun. This product is recommended for skin whitening/lightening applications, as well as anti-aging and anti-wrinkle products.
D-Alpha tocopherol acetate has the structure as give below.
Figure imgf000008_0002
d-alpha-Tocopherol acetate is a closely related chemical with alpha-Tocopherol acetate, d- alpha-tocopherol is a natural active ingredient of Vitamin E, it also has antioxidant potency and reduces oxidative stress induced damages in cells.
D alpha tocopherol succinate
Figure imgf000009_0001
D a-Tocopherol succinate is a fat-soluble vitamin and one of the most potent antioxidant tocopherol. Its antioxidant property helps in reducing oxidative stress induced damages in cells.
Vitamin E or its derivatives are available from BASF and Matrix Lifescience. BASF markets these materials under the names Copherol R1250 and Vitamin E acetate Care.
The present inventors do not fully understand the reason behind the synergistic interaction between Vitamin E and Vitamin C or its derivative to deliver the benefits of the present invention. The extent of scientific understanding, by the present inventors, on these actives and their physiological activity is summarized below.
Mitochondria is known as a powerhouse of the cell as this produces bulk of the cellular ATP. Exposure to external factors like UV, pollution etc., induces reactive oxygen species (ROS) in the cells leading to oxidative stress. The excessive reactive oxygen species causes cellular damage and impacts on mitochondrial functions, thus reduces ATP synthesis. In addition, the reactive oxygen species like superoxide, singlet oxygen, peroxides are also produced during the process of cellular respiration and metabolism under normal condition which could also have undesirable consequences if not quenched by antioxidants. Vitamin C is a water-soluble vitamin having strong antioxidant properties. Apart from direct antioxidant function of Vitamin C, it also enhances endogenous antioxidant enzymes in the cells to reduces oxidative stress induced damages. Hence, vitamin C by its direct antioxidant mechanism and also boosting cellular antioxidant system, negate the effect of oxidative stress induced damages which in turn improves the ATP level in the cell. Vitamin E is fat-soluble antioxidant and used as an important ingredient in many cosmetic products. It protects the skin from various deleterious effects caused by UV exposure by acting as a free-radical scavenger. Many studies suggest that vitamin E has antitumorigenic and photoprotective properties. Since, Vitamin E is a fat-soluble antioxidant, it plays a major role in mitochondrial membrane, which reacts with the peroxyl radicals faster and protects membrane from excessive oxidative damage and thereby enhances mitochondrial function. These unique functions of vitamin C and Vitamin E in reducing oxidative stress and enhancing mitochondrial function may leads to synergistic benefit in boosting ATP levels. The benefit may be further enhanced if the composition additionally includes hydroxystearic acid. It is preferred that the hydroxy stearic acid is 10-hydroxystearic acid, 12-hydroxystearic acid or trihydoxystearic acid (e.g. 9,10,13-trihydroxystearic acid) or trihydroxy stearin or compounds that yield one or more molecules of hydroxy stearic acid or hydroxystearate on their breakdown like mono, di or tri ester of glycerol with hydroxy stearic acid. Of these, 10-hydroxystearic acid, 12- hydroxystearic acid and 9,10,13-trihydroxystearic acid are more preferred, 12-hydroxystearic acid (12-HSA) being most preferred. 12-HSA has the structure as given below:
Figure imgf000010_0001
Hydroxystearic acid is preferably included in 0.001 to 1 %, more preferably 0.01 to 1%, further more preferably 0.1 to 0.5% by weight of the composition.
The composition of the invention may include a humectant which is generally a polyhydric alcohol. Preferably the humectant is at least one of propylene glycol, dipropylene glycol, polypropylene glycol, polyethylene glycol, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1 ,3-butylene glycol, isoprene glycol, 1 ,3 propanediol, pentylene glycol, hexylene glycol, 1 ,2,6-hexanetriol, glycerol, ethoxylated glycerol, and propoxylated glycerol. Preferred humectants are selected from one or more of glycerine, 1 ,3-butylene glycol, propylene glycol, 1 ,3 propanediol, pentylene glycol, hexylene glycol, and sorbitol. Glycerol is the most preferred humectant. It is preferred that the amount of humectant is 0.01 to 10 %, more preferably 0.1 to 5% by weight of the composition.
The topical composition of the invention is meant to be used for personal care or for cosmetic use and could also be referred to as a personal care composition or a cosmetic composition. By a “personal care composition” as used herein, is meant to include a composition for topical application i.e external surfaces of the skin and/or hair of humans. Such a composition may be classified as leave-on or wash off, and includes any product applied to a human body for improving appearance, cleansing, odour control or general aesthetics. The composition is preferably a cleansing composition of the wash off type which comprises a surfactant. The surfactant is preferably an anionic surfactant preferably soap. The anionic surfactant is preferably included in 1 to 80% by weight of the composition. "Skin" as used herein is meant to include skin on the face and body (e.g. neck, chest, back, arms, underarms, hands, legs and scalp) and especially to the exposed parts thereof.
The composition of the invention could also be incorporated in a format for cleansing topical surfaces of the human or animal body. Cleansing composition are of the so called wash-off or rinse-off format. By these formats, is meant that the composition is generally diluted with water and applied on the topical surface e.g. the skin or hair and then rinsed off with copious amounts of water after a few minutes. Cleansing composition could be in any format either in solid form or in liquid form. When in solid form it is preferably a bar which may contain soap, synthetic anionic surfactant or a combination of the two.
The soap for preparing the cleansing composition of the invention is preferably a C8-C24 soap, more preferably C10-C20 soap and most preferably C12-C18 soap. The cation of the soap can be alkali metal, alkaline earth metal or ammonium. Preferably, the cation of the soap is selected from sodium, potassium or ammonium. More preferably the cation of the soap is sodium or potassium. Fatty acids derived from other suitable oils/fats such as groundnut, soybean, tallow, palm, palm kernel, etc. may also be used in other desired proportions.
Synthetic anionic surfactants for use in wash-off compositions are preferably selected from alkyl ether sulphate, primary alkyl sulphate, secondary alkyl sulphonates, alkyl benzene sulphonates, or ethoxylated alkyl sulphates. The anionic surfactant other than soap which is preferred in the cleansing composition is an alkyl ether sulphate preferably those having between 1 and 3 ethylene oxide groups, either from natural or synthetic source and/or sulphonic acid. Especially preferred are sodium lauryl ether sulphates. Alkyl polyglucoside may also be present in the composition, preferably those having a carbon chain length between Ce and C .
When present, the anionic surfactant is preferably present in an amount of 1 to 80%, preferably from 10 to 85%, more preferably 25 to 75% by weight of the cleansing composition. The cleansing composition is preferably in the form of a solid or semi solid form, most preferably in a solid form. Preferred solid compositions are in the shape of a soap bar.
Preferred solid cleansing compositions may include other known ingredients such as perfumes, pigments, preservatives, emollients, sunscreens, gelling agents and thickening agents. Water is a preferred carrier. When water is present, it is preferably present in at least 1%, more preferably at least 2%, further more preferably at least 5% by weight of the composition. When water is the carrier, a preferred cleansing composition comprises 10 to 50%, more preferably 12 to 40%, most preferably 12 to 22% by weight water.
The cleansing composition of the invention may also be delivered through a moisturizing bar or a moisturizing liquid composition. Moisturizing bar compositions comprising fatty acyl isethionates (e.g. cocyl isethionate) are especially preferred. Fatty acyl isethionates (e.g., cocoyl isethionates) surfactant "products" are defined as mixtures of anionic acyl isethionate surfactants and fatty acids/fatty acid soaps. They are highly desirable in personal care skin or hair cleansing products, particularly in personal care products, because they lather well, are mild to the skin and have good emollient properties. Typically, fatty acid isethionate surfactant products are produced by esterification of fatty acids or by reaction of fatty acid chloride having carbon chain length of C8 to C20 with isethionate. A typical surfactant product containing fatty acyl isethionate contains about 40 to 95 wt.% acid isethionate, and 5 to 50 wt.%, typically 10 to 40 wt.% free fatty acid, in addition to isethionate salts, typically at less than 5%, and trace (less than 2 wt.%) of other additives. Fatty acid soap may be included in the range of 5 to 15 wt%. Other surfactants like betaines may be included in 1 to 5 wt%. Water is generally included in 2 to 8 wt% of the composition.
Wash-off composition in the form of a liquid generally comprises surfactants at low concentration and are mild on skin. The surfactant is generally included in 4 to 18%, preferably 6 to 12% by weight of the liquid cleansing composition. Surfactants for inclusion in the liquid cleansing composition of the invention may preferably be of the anionic, non-ionic, cationic or amphoteric types. A useful surfactant for inclusion in the liquid cleansing composition of the invention is sodium lauryl ether sulphate (SLES). The SLES for use in the present invention generally preferably has 1 to 3 Ethoxylate (EO) groups. SLES is preferably included in 3 to 8% by weight of the composition. The other surfactant which may be included in the present invention is cocoamide monoethanol amine (CMEA). CMEA is preferably included in 1 to 3% by weight of the composition. One preferred aspect of the liquid cleansing composition of the invention relates to an aspect wherein the surfactant comprises a mixture of sodium lauryl ether sulphate (SLES) and coco amide monoethanol amine (CMEA). Another useful surfactant for inclusion in the liquid cleansing composition of the invention is an amphoteric surfactant preferably a betaine surfactant, more preferably an alkyl amidopropyl betaine surfactant for example cocamidopropyl betaine. In a preferred embodiment, the composition comprises from 0.1 to 5 wt.%, preferably from 0.5 to 4 wt.%, more preferably from 1 to 3 wt.% of a betaine surfactant. Surfactants of the non-ionic class for inclusion in the liquid cleansing composition of the invention are preferably of the polyoxyethylene sorbitan alkyl esters class (sold as Tween surfactants)), fatty alcohol ethoxylates (sold as Brij surfactants), alkyl phenol ethoxylates (sold as Triton surfactants), fatty acid ethoxylates (sold as Myrj surfactants), and Alkyl poly glucosides (sold as Plantacare surfactants).
Water is a preferred carrier in liquid cleansing compositions of the invention. In such compositions, water is generally present in 70 to 95% by weight.
The composition may also additionally comprise a free fatty acid other than hydroxy stearic acid. The free fatty acid is preferably included in 0.01 to 5% by weight of the composition. The free fatty acid may be one or more of C8-C24, preferably C10-C20 and most preferably C12-C18 fatty acid. The free fatty acids can exist as unsaponified fatty acids in the final product.
Preferred liquid cleansing compositions may include other known ingredients such as electrolytes, perfumes, pigments, preservatives, emollients, sunscreens, emulsifiers, gelling agents and thickening agents.
Another aspect of the present invention relates to a method of providing energizing glow to skin comprising the steps of (i) applying the cleansing composition of the invention on to said skin preferably diluted with water, followed by (ii) rinsing said skin with water.
The method is preferably cosmetic or non-therapeutic.
Yet another aspect of the present invention relates to use of a combination of vitamin C or its derivative and Vitamin E or its derivative to boost ATP levels in HDFa cells.
Use of a composition comprising (i) vitamin C or its derivative and (ii) vitamin E or its derivative, to increase adenosine triphosphate (ATP) levels in the skin as compared to the composition without vitamin C or its derivative and without vitamin E or its derivative; wherein vitamin C or its derivative is selected from the group comprising ascorbic acid, salts of ascorbic acid, oxidized and reduced forms of vitamin C, sodium ascorbyl phosphate, 3-0-Ethyl ascorbic acid, ascorbyl methylsilanol pectinate, magnesium ascorbyl phosphate, ascorbyl glucoside, tetrahexyldecyl ascorbate, aminopropyl ascorbyl phosphate, ascorbyl Palmitate, and mixtures thereof; and wherein vitamin E or its derivative selected from the group comprising a-,p-,y-, and o- tocopherols, their related corresponding tocotrienols and mixtures thereof.
It is preferred that vitamin C or its derivative is included in 0.00001 to 0.002% by weight of the composition.
It is preferred that vitamin E or its derivative is included in 0.00001 to 0.002% by weight of the composition.
It is preferred that the composition additionally comprises 0.001 to 1 wt% hydroxy stearic acid.
It is preferred that the hydroxystearic acid is 12-hydroxystearic acid.
It is preferred that the composition is a cleansing composition comprising a surfactant.
It is preferred that the surfactant comprises an anionic surfactant preferably soap.
It is preferred that the anionic surfactant is included in 1 to 80 wt% of the composition.
It is preferred that the composition comprises a humectant preferably glycerol.
The present invention provides a cleansing composition comprising
0.00001 to 0.002 wt% vitamin C or its derivative;
0.00001 to 0.002 wt% vitamin E or its derivative;
1 to 80 wt% anionic surfactant; and
10 to 90 wt% water.
It is preferred that the composition additionally comprises 0.001 to 1 wt% hydroxystearic acid.
It is preferred that the composition additionally comprises 0.01 to 5 wt% free fatty acid other than said hydroxystearic acid.
It is preferred that the composition additionally comprises a humectant preferably glycerol. The present invention provides a method increase adenosine triphosphate (ATP) levels in the skin comprising the steps of (i) applying a composition as claimed in any one of the preceding claims 10 to 13 on to said skin preferably diluted with water, followed by (ii) rinsing said skin with water.
The invention will now be illustrated with the help of the following non-limiting examples.
Examples
Examples A - F and 1-3: Interaction between Vitamin C and Vitamin E to deliver synergistic ATP levels
Individual actives as well as combination of actives as shown in Table -1 as shown below were prepared. They were tested for ATP levels in HDFa cells using the protocol as given below:
Human dermal fibroblast (HDFa) cells were seeded in 96 well plate and maintained in a 5% CO2 incubator at 37°C for 24 h. Then cells were treated with different concentrations of Vitamin C (VC) or Vitamin E (VE) or a combination of VC+VE with fresh media and incubation was continued for 1 h. Post 1 h of incubation, cells were lysed by adding 30 pl of RIPA buffer with protease and phosphatase inhibitor. The cell lysate was used for estimating ATP levels using ATP determination Kit. The ATP data is normalized with total protein from the respective treatments and the ATP levels were measured with and without treatment.
The ATP levels as measured are also shown in Table -1 .
Table -1
Figure imgf000015_0001
The data in Table - 1 above indicates that the combination of Vitamin C and Vitamin E at any concentration (from 0.01 to 1 ppm) is superior to that of using an individual active at the same concentration, thereby indicating synergistic activity.
A cleaning composition as given in Table -2 below was found to be useful for delivering the energizing glow by way of the present invention.
Table - 2
Figure imgf000016_0001

Claims

Claims
1. Use of a composition comprising (i) vitamin C or its derivative and (ii) vitamin E or its derivative, to increase adenosine triphosphate (ATP) levels in the skin as compared to the composition without vitamin C or its derivative and without vitamin E or its derivative; wherein vitamin C or its derivative is selected from the group comprising ascorbic acid, salts of ascorbic acid, oxidized and reduced forms of vitamin C, sodium ascorbyl phosphate, 3-O-Ethyl ascorbic acid, ascorbyl methylsilanol pectinate, magnesium ascorbyl phosphate, ascorbyl glucoside, tetrahexyldecyl ascorbate, aminopropyl ascorbyl phosphate, ascorbyl Palmitate, and mixtures thereof; and wherein vitamin E or its derivative selected from the group comprising a-,p-,y-, and o- tocopherols, their related corresponding tocotrienols and mixtures thereof.
2. Use as claimed in claim 1 wherein vitamin C or its derivative is included in 0.00001 to 0.002% by weight of the composition.
3. Use as claimed in claim 1 or 2 wherein vitamin E or its derivative is included in 0.00001 to 0.002% by weight of the composition.
4. Use as claimed in any one of the preceding claims wherein the composition additionally comprises 0.001 to 1 wt% hydroxy stearic acid.
5. Use as claimed in claim 4 wherein the hydroxy stearic acid is 12-hydroxystearic acid.
6. Use as claimed in any one of the preceding claims wherein the composition is a cleansing composition comprising a surfactant.
7. Use as claimed in claim 6 wherein the surfactant comprises an anionic surfactant preferably soap.
8. Use as claimed in claim 7 wherein the anionic surfactant is included in 1 to 80 wt% of the composition. Use as claimed in any one of the preceding claims comprising a humectant preferably glycerol. A cleansing composition comprising
(i) 0.00001 to 0.002 wt% vitamin C or its derivative;
(ii) 0.00001 to 0.002 wt% vitamin E or its derivative;
(iii) 1 to 80 wt% anionic surfactant; and
(iv) 10 to 90 wt% water. A composition as claimed in claim 10 additionally comprising 0.001 to 1 wt% hydroxystearic acid. A composition as claimed in claim 11 additionally comprising 0.01 to 5 wt% free fatty acid other than said hydroxystearic acid. A composition as claimed in any one of claims 10 to 12 additionally comprising a humectant preferably glycerol. A method increase adenosine triphosphate (ATP) levels in the skin comprising the steps of (i) applying a composition as claimed in any one of the preceding claims 10 to 13 on to said skin preferably diluted with water, followed by (ii) rinsing said skin with water.
PCT/EP2023/080541 2022-11-24 2023-11-02 Use of a composition for providing energizing glow on skin WO2024110164A1 (en)

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Citations (5)

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Publication number Priority date Publication date Assignee Title
WO2009138801A2 (en) 2008-05-14 2009-11-19 Nb Labs Limited Cosmetic formulation
EP2637636A1 (en) 2010-11-11 2013-09-18 Unilever PLC Leave-on nonsolid skin conditioning compositions containing 12-hydroxystearic acid
WO2014041528A2 (en) * 2012-09-14 2014-03-20 Martin Roslain Margaret A composition
US20180325804A1 (en) 2017-05-12 2018-11-15 Shaklee Corporation Topical muscadine formulation for cosmetic use
US20220218594A1 (en) 2018-12-14 2022-07-14 Mary Kay Inc. Cosmetic compositions

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009138801A2 (en) 2008-05-14 2009-11-19 Nb Labs Limited Cosmetic formulation
EP2637636A1 (en) 2010-11-11 2013-09-18 Unilever PLC Leave-on nonsolid skin conditioning compositions containing 12-hydroxystearic acid
EP2637636B1 (en) * 2010-11-11 2019-02-20 Unilever PLC Leave-on nonsolid skin conditioning compositions containing 12-hydroxystearic acid
WO2014041528A2 (en) * 2012-09-14 2014-03-20 Martin Roslain Margaret A composition
US20180325804A1 (en) 2017-05-12 2018-11-15 Shaklee Corporation Topical muscadine formulation for cosmetic use
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Title
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MINTEL, CLARIFYING FACIAL CLEANSER, 21 August 2022 (2022-08-21)

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