WO2024109896A1 - Dispositif de régulation rapide de la température pour la culture cellulaire, fondé sur une puce microfluidique - Google Patents

Dispositif de régulation rapide de la température pour la culture cellulaire, fondé sur une puce microfluidique Download PDF

Info

Publication number
WO2024109896A1
WO2024109896A1 PCT/CN2023/133765 CN2023133765W WO2024109896A1 WO 2024109896 A1 WO2024109896 A1 WO 2024109896A1 CN 2023133765 W CN2023133765 W CN 2023133765W WO 2024109896 A1 WO2024109896 A1 WO 2024109896A1
Authority
WO
WIPO (PCT)
Prior art keywords
water
temperature control
water bath
bath incubator
chip
Prior art date
Application number
PCT/CN2023/133765
Other languages
English (en)
Chinese (zh)
Inventor
黄术强
沈雅欣
李思宏
于跃
马智鑫
王金娟
李雪飞
Original Assignee
中国科学院深圳先进技术研究院
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 中国科学院深圳先进技术研究院 filed Critical 中国科学院深圳先进技术研究院
Publication of WO2024109896A1 publication Critical patent/WO2024109896A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control

Definitions

  • the invention relates to the field of biotechnology, and in particular to a rapid temperature control device based on microfluidic chip cell culture.
  • Microfluidic chip technology combined with time-lapse microscopy technology can achieve long-term monitoring and cultivation of single cells.
  • temperature conditions suitable for biological growth are usually required. Different cells or microorganisms may not necessarily have the same growth conditions, including but not limited to temperature conditions. Controlling the rapid changes in temperature in the microfluidic chip and its surrounding environment is the key to the process.
  • the first is a temperature control box that can be integrated on a microscope. It controls the temperature of the microfluidic chip on the microscope stage by heating the air to form convection, which can achieve a larger temperature control range; however, the temperature distribution uniformity is poor, and the air heating volume in a large space causes the temperature to rise and fall very slowly, and it is impossible to achieve a fast and stable temperature change effect.
  • the second is a small constant temperature incubator based on metal heating that can be placed on the microscope stage, which directly heats or cools the microfluidic chip placed therein; the small constant temperature incubator can control the overall microfluidic chip to achieve a relatively fast heating process, but because it has no heat dissipation device, it takes a long time to wait during the cooling process.
  • the microfluidic chip is in the air environment inside the incubator, and the heating rate is still limited by the large specific heat capacity of the air.
  • the third method is to use semiconductor cooling chips placed directly under the microfluidic chip to perform heating or cooling operations through current control. Some research institutions also directly print thermoelectric semiconductor temperature control circuits on microfluidic chips to control the temperature on the chip.
  • Semiconductor cooling chips usually need to be placed under the microfluidic chip, but the activity space of the microscope lens will be limited, so high-throughput experimental data acquisition cannot be achieved.
  • the semiconductor cooling chip does not directly contact the microfluidic chip, uneven temperature conduction leads to poor temperature uniformity at different locations on the microfluidic chip.
  • it is required to be a special hard insulating sheet chip substrate, which has high requirements for thermoelectric materials.
  • High-power microscopes usually use glass with a thickness of 0.17 mm as the chip substrate. This type of semiconductor temperature control method has a limited application range.
  • microfluidic chips using semiconductor cooling methods are usually in contact with room temperature air, so microfluidic chips cannot stably maintain the expected temperature.
  • the present invention provides a rapid temperature control device based on microfluidic chip cell culture, the rapid temperature control device is compatible with a time-lapse microscopic imaging system, and comprises: a water bath incubator, which is arranged above a microscope stage or an electric displacement stage of the time-lapse microscopic imaging system; the water bath incubator comprises a box body and a water inlet and a water outlet arranged on the box body, a hollow window is arranged at the bottom of the box body, a transparent window is arranged at the top of the box body, and the centers of the hollow window and the transparent window are both coaxial with the objective lens optical axis of the time-lapse microscopic imaging system; a microfluidic chip is arranged on the hollow window of the water bath incubator, and a glass sheet substrate of the microfluidic chip is connected to the hollow window.
  • a water bath incubator which is arranged above a microscope stage or an electric displacement stage of the time-lapse microscopic imaging system
  • the water bath incubator comprises a box body and a water inlet and
  • a temperature sensing probe is arranged in the water bath incubator and close to the microfluidic chip or on the microfluidic chip;
  • a temperature control system includes a water reservoir, a thermostat, a water inlet pipe connected to the water inlet, and a water outlet pipe connected to the water outlet; wherein the thermostat is connected to the temperature sensing probe to monitor the ambient temperature in the water bath incubator; the thermostat is used to make the water in the water reservoir reach the target temperature and maintain a constant temperature, the constant temperature water in the water reservoir enters the water bath incubator through the water inlet pipe and flows out of the water reservoir through the water outlet pipe, thereby realizing the circulation of constant temperature water in the water bath incubator to stabilize the temperature control.
  • the box body includes a base and a top cover arranged on the base, the hollow window is arranged on the base, and the transparent window is arranged on the top cover.
  • the hollow window is designed to allow a high-power objective lens to image the maximum area of the microfluidic chip.
  • the rapid temperature control device further comprises a chip pressing sheet disposed in the water bath incubator and detachably connected to the base, wherein the chip pressing sheet comprises a spring pin or a rubber ring for pressing the microfluidic chip.
  • the chip pressing piece is connected to the base via screws.
  • a waterproof rubber ring is provided on the peripheral side of the chip pressing sheet, a rubber ring groove is opened on the peripheral side of the upper surface of the base, and a waterproof rubber ring matching with the rubber ring groove is provided on the lower surface of the top cover.
  • the base and the top cover are connected by screws.
  • the box body is also provided with a chip pipeline inlet and a chip pipeline outlet.
  • the chip pipeline inlet and the chip pipeline outlet are arranged on the top cover.
  • the temperature control system further comprises a peristaltic pump, and the water inlet pipe is connected to the peristaltic pump so as to facilitate the transmission of the constant temperature water in the water reservoir to the interior of the water bath incubator.
  • the present invention provides a rapid temperature control device for cell culture based on a microfluidic chip, which is compatible with a time-lapse microscopy imaging system, and includes a water bath incubator, a temperature control system, and a microfluidic chip and a temperature sensing probe arranged in the water bath incubator.
  • the water bath incubator is arranged above a microscope stage or an electric displacement stage of the time-lapse microscopy imaging system, a hollow window is arranged at the bottom of the water bath incubator, and a transparent window is arranged at the top of the water bath incubator, and the centers of the transparent window and the hollow window are coaxial with the optical axis of the objective lens of the time-lapse microscopy imaging system;
  • the microfluidic chip is arranged on the hollow window, and its glass sheet base is closely matched with the hollow window;
  • the thermostat of the temperature control system is connected to the temperature sensing probe to monitor the ambient temperature in the water bath incubator; the thermostat is used to heat the water in the water reservoir to reach the target temperature and maintain a constant temperature, and the constant temperature water in the water reservoir enters the water bath incubator through a water inlet pipe and flows out of the water reservoir through a water outlet pipe, thereby realizing the circulation of constant temperature water in the water bath incubator to stabilize the temperature control.
  • the cell culture fluid enters the microfluidic chip through the chip pipeline in
  • FIG1 is a schematic structural diagram of an embodiment of a rapid temperature control device for cell culture based on a microfluidic chip provided in the present application.
  • FIG. 2 is a schematic diagram of the exploded structure of the water bath incubator in the embodiment provided in FIG. 1 .
  • first and second are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as “first” and “second” may explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of “multiple” is two or more, unless otherwise clearly and specifically defined.
  • the present application provides a rapid temperature control device for cell culture based on a microfluidic chip.
  • the rapid temperature control device is compatible with a time-lapse microscopy imaging system. It includes a water bath incubator 10, a temperature control system 20, and a microfluidic chip and a temperature sensing probe 12 arranged in the water bath incubator.
  • the temperature sensing probe 12 is placed near or on the microfluidic chip for real-time temperature monitoring.
  • the water bath incubator 10 is arranged above the microscope stage A or the electric translation stage of the time-lapse microscopy imaging system. It includes a box body and a water inlet 144 and a water outlet 145 arranged on the box body.
  • a hollow window 111 is arranged at the bottom of the box body, and a transparent window 141 is arranged at the top of the box body.
  • the centers of the transparent window 141 and the hollow window 111 are coaxial with the optical axis of the objective lens B of the time-lapse microscopy imaging system.
  • the lighting requirements are achieved by opening windows at the top and bottom of the box body.
  • the box body includes a base 11 and a top cover 14 disposed on the base 11 , and the base 11 and the top cover 14 form a containing chamber.
  • the base 11 is a groove structure and is compatible with the microscope stage A or the electric translation stage for time-lapse microscopic imaging experiments and applications.
  • the base 11 with a groove structure can be filled with an aqueous solution to immerse the microfluidic chip disposed inside the water bath incubator 10.
  • a hollow window 111 with a groove structure for supporting the microfluidic chip is provided in the middle of the base 11, and the glass sheet substrate of the microfluidic chip is tightly matched with the hollow window 111.
  • the design of the hollow window 111 satisfies the maximum area imaging of the microfluidic chip by a high-power objective lens. It can be understood that the size and shape of the base 11 can be adjusted and designed according to the application platform to match with different types and models of stages or electric translation stages.
  • a chip pressing plate 13 is also included, which is arranged in the water bath incubator 10 and is detachably connected to the base 11.
  • the chip pressing plate 13 is connected to the base 11 by screws, and a spring pin 131 for pressing the glass substrate of the microfluidic chip is provided on the chip pressing plate 13.
  • the glass substrate of the microfluidic chip is adapted to the hollow window 111.
  • the spring pins 131 on the chip pressing plate 13 are evenly distributed directly above the hollow window 111, that is, directly above the glass substrate of the microfluidic chip. Through the squeezing of the spring pins 131, the glass substrate of the microfluidic chip is tightly fixed to the base 11.
  • the top cover 14 is a convex groove structure corresponding to the base 11, and a transparent window 141 is provided in the middle thereof, and a transparent plate 142 is fixed in the transparent window 141.
  • the centers of the transparent window 141 of the top cover 14 and the hollow window 111 of the base 11 are coaxial with the optical axis of the objective lens B of the time-lapse microscopy imaging system.
  • the transparent window 141 can project incident light onto the microfluidic chip in the water bath incubator 10.
  • the transparent window 141 is large enough to avoid blocking the incident light.
  • the distance between the objective lens B and the sample is only a few hundred microns, and the objective lens oil is filled in the middle.
  • the objective lens B needs sufficient space to ensure a large range of sampling.
  • the design of the hollow window 111 retains the imaging space of the microfluidic chip to the maximum extent.
  • a water inlet 144, a water outlet 145, a chip pipeline inlet 147 and a chip pipeline outlet 146 are provided on one side of the top cover 14.
  • the first pipeline 24 connected to the culture liquid tube C enters the water bath incubator 10 from the chip pipeline inlet 147 of the top cover 14 and is connected to the microfluidic chip.
  • the microfluidic core is connected to the second pipeline 25.
  • the second pipeline 25 passes through the water bath incubator 10 from the chip pipeline outlet 146 of the top cover 14 and is connected to the waste liquid pipe D, so as to provide the nutritional environment required for continuous cell culture. It can be understood that the water inlet 144, the water outlet 145, the chip pipeline inlet 146 and the chip pipeline outlet 147 can also be set at other positions of the top cover 14 according to actual needs.
  • the first pipeline 24 and the second pipeline 25 can be placed in a non-window space position in the water bath incubator 10 as required, and the outer sides of the chip pipeline inlet 147 and the chip pipeline outlet 146 are sealed and fixed to the top cover 14 with pipeline blade rings and split joints.
  • the top cover 14 and the base 11 are provided with corresponding threaded holes 113 around them, so that the two can be connected by screws. Furthermore, a rubber ring groove 112 is provided on the upper surface of the base 11, and a waterproof rubber ring 143 that cooperates with the rubber ring groove 112 is provided on the lower surface of the top cover 14. The waterproof rubber ring 143 is squeezed by screwing the base 11 and the top cover 14 to waterproof the connection between the two.
  • the base 11 and the top cover 14 are combined into a water bath incubator 10 with a water storage function, which can quickly control the temperature of the microfluidic chip immersed therein.
  • the temperature control system 20 includes a water reservoir 21, a thermostat 22, a peristaltic pump 23, a water inlet pipe 26 connected to the water inlet 144, and a water outlet pipe 27 connected to the water outlet 145.
  • the water inlet pipe 26 and the water outlet pipe 27 are placed together in the water reservoir 21.
  • the water inlet pipe 26 is connected to the peristaltic pump 23 so that the constant temperature water in the water reservoir 21 is transferred to the water bath incubator 10 so that the water fills the inside of the water bath incubator 10.
  • the thermostat 22 is used to make the water in the water reservoir 21 reach the target temperature and maintain the constant temperature.
  • the constant temperature water enters the water bath incubator 10 through the water inlet pipe 26 and flows out of the water reservoir 21 through the water outlet pipe 27, thereby realizing the circulation of the constant temperature water in the water bath incubator 10 to stabilize the temperature control; the thermostat 22 is connected to the temperature sensing probe 12 to monitor the ambient temperature in the water bath incubator 10. If the ambient temperature of the microfluidic chip needs to be changed quickly, the target temperature of the thermostat 22 can be adjusted and set to quickly replace the water in the water reservoir 21 with water of the target temperature for circulation, so that the monitoring temperature of the microfluidic chip reaches the target temperature.
  • the temperature disturbance of the microfluidic chip in the water bath environment is extremely small, the temperature switching is accurate and stable, and rapid temperature change can be achieved. The temperature switching is completed within 3 minutes and maintained stable, and the temperature range can reach 50°C.
  • the number and position of the chip pipeline inlet 147, the chip pipeline outlet 146, the water inlet 144 and the water outlet 145 of the water bath incubator 10 can be arbitrarily designed and changed under the premise of satisfying rapid temperature change.
  • the present application provides a rapid temperature control device for cell culture based on a microfluidic chip.
  • the rapid temperature control device is compatible with a time-lapse microscopy imaging system and includes a water bath incubator 10, a temperature control system 20, and a microfluidic chip and a temperature sensing probe 12 arranged in the water bath incubator 10.
  • the water bath incubator 10 is arranged above the microscope stage A or the electric translation stage of the time-lapse microscopy imaging system, a hollow window 111 is arranged at the bottom of the water bath incubator 10, and a transparent window 141 is arranged at the top of the water bath incubator 10, and the centers of the transparent window 141 and the hollow window 111 are coaxial with the optical axis of the objective lens B of the time-lapse microscopy imaging system; the microfluidic chip is arranged on the hollow window 111, and its glass sheet base is tightly matched with the hollow window 111; the thermostat 22 of the temperature control system 20 is connected to the temperature sensing probe 12 to monitor the ambient temperature in the water bath incubator 10; the thermostat 22 is used to make the water in the water reservoir 21 reach the target temperature and maintain a constant temperature, the constant temperature water in the water reservoir 21 enters the water bath incubator 10 through the water inlet pipe 26 and flows out of the water reservoir 21 through the water outlet pipe 27, thereby realizing the circulation of constant temperature water in the water bath incubator 10 to stabilize the temperature control
  • the rapid temperature control device has been realized and its feasibility and effectiveness have been verified by long-term experiments.
  • the rapid temperature control device was used to culture Escherichia coli cells at 37°C for 6 hours and then switched to 10°C within 3 minutes to obtain time-lapse microscopy experimental results.
  • the experimental data obtained can be analyzed and counted using image analysis software.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention propose un dispositif de régulation rapide de la température pour la culture cellulaire fondé sur une puce microfluidique, comprenant un incubateur à bain d'eau, un système de régulation de la température, ainsi qu'une puce microfluidique et une sonde de détection de la température qui sont présentes dans l'incubateur à bain d'eau. L'incubateur à bain d'eau est agencé sur une platine de microscope ou une platine de déplacement motorisée d'un système d'imagerie de microscopie à laps de temps, une fenêtre creuse est présente au bas d'un corps d'incubateur de l'incubateur à bain d'eau, une fenêtre transparente est présente au sommet du corps d'incubateur, et les centres de la fenêtre transparente et de la fenêtre creuse sont tous deux coaxiaux avec l'axe optique d'une lentille d'objectif du système d'imagerie de microscopie à laps de temps ; un substrat en feuille de verre de la puce microfluidique est en étroite adéquation avec la fenêtre creuse ; un thermostat du système de régulation de la température est connecté à la sonde de détection de la température de manière à surveiller la température ambiante dans l'incubateur à bain d'eau ; le thermostat est utilisé pour chauffer l'eau dans un réservoir de stockage de l'eau à une température cible et pour maintenir une température constante, et l'eau à température constante dans le réservoir de stockage de l'eau entre dans l'incubateur à bain d'eau par un tuyau d'entrée d'eau et s'écoule ensuite hors du réservoir de stockage de l'eau par un tuyau de sortie d'eau, de manière à ce que l'eau à température constante circule à l'intérieur de l'incubateur à bain d'eau afin d'obtenir une régulation stable de la température.
PCT/CN2023/133765 2022-11-24 2023-11-23 Dispositif de régulation rapide de la température pour la culture cellulaire, fondé sur une puce microfluidique WO2024109896A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202211479951.2A CN115747044A (zh) 2022-11-24 2022-11-24 一种基于微流控芯片细胞培养的快速控温装置
CN202211479951.2 2022-11-24

Publications (1)

Publication Number Publication Date
WO2024109896A1 true WO2024109896A1 (fr) 2024-05-30

Family

ID=85336739

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2023/133765 WO2024109896A1 (fr) 2022-11-24 2023-11-23 Dispositif de régulation rapide de la température pour la culture cellulaire, fondé sur une puce microfluidique

Country Status (2)

Country Link
CN (1) CN115747044A (fr)
WO (1) WO2024109896A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115747044A (zh) * 2022-11-24 2023-03-07 中国科学院深圳先进技术研究院 一种基于微流控芯片细胞培养的快速控温装置
CN116699366A (zh) * 2023-06-14 2023-09-05 深圳市艾格林电子有限公司 一种多模式集成电路芯片性能评估装置及方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN209555253U (zh) * 2019-01-24 2019-10-29 大连理工大学 一种分体式微流控芯片细胞动态培养辅助装置
CN115747044A (zh) * 2022-11-24 2023-03-07 中国科学院深圳先进技术研究院 一种基于微流控芯片细胞培养的快速控温装置

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN209555253U (zh) * 2019-01-24 2019-10-29 大连理工大学 一种分体式微流控芯片细胞动态培养辅助装置
CN115747044A (zh) * 2022-11-24 2023-03-07 中国科学院深圳先进技术研究院 一种基于微流控芯片细胞培养的快速控温装置

Also Published As

Publication number Publication date
CN115747044A (zh) 2023-03-07

Similar Documents

Publication Publication Date Title
WO2024109896A1 (fr) Dispositif de régulation rapide de la température pour la culture cellulaire, fondé sur une puce microfluidique
US4974952A (en) Live cell chamber for microscopes
KR101741650B1 (ko) 현미경 관찰용 배양장치 및 그 사용 방법
US5451524A (en) In vitro chamber for human organ tissue samples
US5181382A (en) Heating/cooling or warming stage assembly with coverslip chamber assembly and perfusion fluid preheater/cooler assembly
US20080131922A1 (en) Cell culture detection apparatus, cell culture observation apparatus, and cell culture observation method
US5343018A (en) Microscope lens and stage heater with flexible objective lens casing heater sleeve
JP4823557B2 (ja) 顕微鏡装置
US9675974B2 (en) Cap for monitoring objects in suspension
US7502165B2 (en) Arrangement for regulating the temperature of the sample space of a microscope
WO2005095576A2 (fr) Incubateur a etage microscope pour une enveloppe d'eau (ou tout autre fluide) contenant du co2 a temperature commandee
CN105602846A (zh) 一种小型化空间实验用细胞培养装置
JPH1028576A (ja) 顕微鏡観察用透明恒温培養容器
Rieder et al. Perfusion chambers for high-resolution video light microscopic studies of vertebrate cell monolayers: some considerations and a design
JP2004113092A (ja) 細胞培養チップ
JP5062587B2 (ja) ワンタッチ式細胞動態観察装置
Ho et al. Mini chamber system for long-term maintenance and observation of cultured cells
JP4117341B2 (ja) 温水循環式顕微培養チャンバー
WO2005097325A1 (fr) Dispositif autonome a regulation thermique active
KR100745110B1 (ko) 계속적 현미경 관찰이 가능한 세포 배양장치
WO2024116681A1 (fr) Dispositif de congélation, microscope, procédé de congélation et procédé d'observation
Yuan et al. A flexible chamber for time-lapse live-cell imaging with stimulated raman scattering microscopy
US20200325434A1 (en) Incubator plate for use in microscopy system
JP2005333912A (ja) 小型培養観察装置
CN114295399B (zh) 一种均热板蒸发器散热性能的可视化测试装置