WO2024103297A1 - 样品提取卡盒和样品提取方法及核酸检测设备 - Google Patents

样品提取卡盒和样品提取方法及核酸检测设备 Download PDF

Info

Publication number
WO2024103297A1
WO2024103297A1 PCT/CN2022/132302 CN2022132302W WO2024103297A1 WO 2024103297 A1 WO2024103297 A1 WO 2024103297A1 CN 2022132302 W CN2022132302 W CN 2022132302W WO 2024103297 A1 WO2024103297 A1 WO 2024103297A1
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
liquid
sample extraction
mixing
switch valve
Prior art date
Application number
PCT/CN2022/132302
Other languages
English (en)
French (fr)
Inventor
蒋太交
梁松松
李胜光
耿鹏
陈绮娴
张辉
马然
廖宇飞
Original Assignee
广州国家实验室
生物岛实验室
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 广州国家实验室, 生物岛实验室 filed Critical 广州国家实验室
Priority to PCT/CN2022/132302 priority Critical patent/WO2024103297A1/zh
Publication of WO2024103297A1 publication Critical patent/WO2024103297A1/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

Definitions

  • the present invention relates to the technical field of biomedical instruments, and in particular to a sample extraction card box, a sample extraction method and a nucleic acid detection device.
  • Biomolecule detection involves the transfer of liquids and specific operations. In the transfer process, most of them adopt open-type aspiration, transfer, or pouring between reagent tubes, which can easily cause volatile toxic reagents to evaporate and diffuse to the outside of the container, pollute the external environment, and endanger the personal safety of operators.
  • the present invention provides a sample extraction card box, a sample extraction method and a nucleic acid detection device, and the technical solution adopted is as follows.
  • the sample extraction card box nucleic acid detection device provided by the present invention comprises a sample extraction card box.
  • the sample extraction cartridge comprises a cartridge base and a plurality of chamber combinations, wherein the chamber combination is connected to the cartridge base, and the chamber combination comprises a first chamber, a second chamber, a first mixing chamber, a second mixing chamber, a third chamber and a fourth chamber, wherein the first chamber can be used to store a preservation solution, and the first chamber is connected to the cartridge base; the second chamber is connected to the cartridge base, and the second chamber can be used to store a washing solution, and the second chamber is provided with a second plunger; the first mixing chamber is connected to the cartridge base, and the first mixing chamber is provided with a first mixing plunger; the second mixing chamber is connected to the cartridge base, and the second mixing chamber can be used to store a lysate, and the second mixing chamber is provided with a second mixing plunger;
  • the third chamber is connected to the cartridge base, and the third chamber is used to connect to the gas source; the fourth chamber is connected to the cartridge base, and the fourth chamber can be used to store eluent, and the fourth
  • a magnetic element accommodating area is provided between the first mixing chamber and the second mixing chamber.
  • the sample extraction cartridge includes a magnetic element, and the magnetic element is configured to move toward or away from a magnetic suction chamber of the cartridge base in a direction perpendicular to a surface of the cartridge base in the magnetic element accommodating area.
  • the chamber combination also includes a fifth chamber, the fifth chamber is connected to the cartridge base, the fifth chamber is used to store mineral oil or gas, the fifth chamber is provided with a fifth plunger, the fifth chamber is connected to the third liquid circuit, and the connection between the fifth chamber and the third liquid circuit can be opened and closed.
  • the sample extraction cartridge comprises a plurality of switch valves, each of which is slidably connected to the cartridge base; the switch valves are used to synchronously control the on-off of the liquid circuits in each of the liquid circuit assemblies.
  • the liquid path combination is respectively disposed on two opposite sides of the cartridge base, and the first liquid path, the second liquid path, and the third liquid path are symmetrically disposed on two opposite sides of the cartridge base.
  • a plurality of flow paths are provided in the switch valve, and the flow paths form a first connecting port and a second connecting port on the side of the switch valve, the first connecting ports are arranged in one row or two rows on the upper side of the switch valve, and the second connecting ports are respectively located on two opposite sides of the switch valve.
  • the sample extraction cartridge includes a plurality of covers, and the covers are connected to the first chamber in a separate or integrated manner.
  • a filter element is disposed in the cover body.
  • the third chamber is provided with a filter element.
  • the second mixing plunger is configured with a stroke control element, and the stroke control element controls the moving distance of the second mixing plunger.
  • the sample extraction cartridge comprises four of the chamber assemblies and four of the fluid path assemblies.
  • the sample extraction method provided by the present invention uses a sample extraction cartridge, and the extraction process of the sample extraction method includes magnetic bead activation, sample addition, lysis reaction, washing and elution, and the on-off of the liquid circuits in the plurality of parallel liquid circuit combinations is synchronously controlled by the slidingly connected switch valves to complete the transfer of the fluid.
  • the present invention can be widely applied to the field of biomedical instrument technology.
  • the embodiments of the present invention have at least the following beneficial effects:
  • Each chamber in the chamber combination of the sample extraction card box is opened and closed correspondingly with each liquid path in the liquid path combination, so as to continuously achieve sample liquid mixing, washing, residual liquid cleaning, elution and liquid discharge in a closed environment, thereby improving extraction and detection efficiency, and the waste liquid generated can be stored in the first chamber, thereby improving structural utilization.
  • the sample extraction cartridge is designed to have multiple chamber combinations and multiple liquid path combinations, and each liquid path combination is connected in parallel, thereby obtaining a multi-channel parallel cartridge structure to prevent cross-flow, and can simultaneously process multiple sample liquids, further improving efficiency.
  • a switch valve is designed in the cartridge base, and the switch valve can slide and change position, thereby controlling the on-off between each liquid circuit in the liquid circuit combination, and the switch valve can simultaneously control the liquid circuits in each liquid circuit combination, making the operation more convenient.
  • the liquid path combinations are symmetrically arranged on opposite sides of the cartridge base, which are independent of each other to prevent cross-liquid flow, and the mixing or flowing time of the sample liquid in each liquid path combination is consistent to ensure the accuracy of the detection.
  • FIG. 1 is a structure diagram of an embodiment of a sample extraction cartridge.
  • FIG. 2 is a structural diagram of the sample extraction cartridge in FIG. 1 , showing that each switch valve is disassembled from the cartridge base and the chambers are combined into four.
  • FIG. 3 shows 12 sample extraction cartridges arranged in a 4*12 layout.
  • FIG. 4 shows 24 sample extraction cartridges arranged symmetrically in two rows in a 4*12*2 layout, with 4 in the horizontal direction and 24 in the vertical direction.
  • FIG5 shows 24 sample extraction cartridges arranged symmetrically in two rows in a 4*12*2 layout, with 8 in the horizontal direction and 12 in the vertical direction.
  • first switch valve 1202, second switch valve; 1203, third switch valve; 1204, fourth switch valve; 1205, fifth switch valve;
  • FIGS. 1 to 5 examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions.
  • the embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and are not to be construed as limiting the present invention.
  • the terms “installed”, “connected”, and “connected” should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components.
  • installed should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components.
  • the invention relates to a nucleic acid detection device, which comprises a sample extraction card box.
  • the nucleic acid sample liquid is discharged after being cracked, washed and eluted in the sample extraction card box for detection.
  • the present invention relates to a sample extraction cartridge, which comprises a cartridge base 1000 and a plurality of chamber combinations, wherein the chamber combination comprises a first chamber 2101, a second chamber 2102, a first mixing chamber 2201, a second mixing chamber 2202, a third chamber 2103 and a fourth chamber 2104.
  • the first chamber 2101, the second chamber 2102, the first mixing chamber 2201, the second mixing chamber 2202, the third chamber 2103 and the fourth chamber 2104 are respectively connected to the cartridge base 1000.
  • the first chamber 2101 can be used to store a preservation solution
  • a throat swab with sample solution is placed in the first chamber 2101 and mixed with the preservation solution
  • the second chamber 2102 can be used to store a washing solution
  • the second mixing chamber 2202 can be used to store a lysate
  • the lysate is mixed with magnetic beads
  • the third chamber 2103 is used to connect to a gas source
  • the fourth chamber 2104 can be used to store an eluent.
  • the cartridge base 1000 is provided with a plurality of liquid circuit combinations, and the liquid circuit combinations are used to achieve the connection between the chambers in the chamber combination.
  • the liquid circuit combination includes a first liquid circuit 1101, a second liquid circuit 1102, a third liquid circuit 1103, and a fourth liquid circuit 1104.
  • Each liquid circuit is formed by micro-nano processing, and is a liquid circuit at the micron level.
  • Each liquid circuit in the liquid circuit combination can be opened and closed separately to achieve the connection between different chambers.
  • each liquid circuit combination is connected in parallel, and each chamber combination forms a parallel and independent cartridge structure through the corresponding liquid circuit combination.
  • the structure is compact, and there is no cross-talk between the liquids. It can process multiple nucleic acid samples at the same time, thereby improving efficiency.
  • the first liquid path 1101 is used to connect the first chamber 2101, the second chamber 2102 and the first mixing chamber 2201 respectively. Specifically, the first chamber 2101 and the second chamber 2102 can be connected to the first liquid path 1101 and disconnected respectively.
  • the second liquid path 1102 is used to connect the first mixing chamber 2201 and the second mixing chamber 2202 respectively.
  • the first mixing chamber 2201 can be switched to connect to the first liquid path 1101 or the second liquid path 1102. It can be understood that if the first mixing chamber 2201 is switched to connect to the second liquid path 1102, the first mixing chamber 2201 is disconnected from the first liquid path 1101.
  • the first mixing chamber 2201 is switched to be connected to the first liquid path 1101, the first mixing chamber 2201 is disconnected from the second liquid path 1102.
  • the first chamber 2101 is connected to the first liquid path 1101 and the second chamber 2102 is disconnected from the first liquid path 1101, the first chamber 2101 is connected to the first mixing chamber 2201, and the liquid can flow between the first chamber 2101 and the first mixing chamber 2201;
  • the second chamber 2102 is connected to the first liquid path 1101 and the first chamber 2101 is connected to the first liquid path 1101, the second chamber 2102 is connected to the first mixing chamber 2201, and the liquid can flow between the second chamber 2102 and the first mixing chamber 2201.
  • the third liquid circuit 1103 is used to connect the second mixing chamber 2202, the third chamber 2103, the fourth chamber 2104 and the fourth liquid circuit 1104 respectively.
  • the second mixing chamber 2202 can be switched to connect to the second liquid circuit 1102 or the third liquid circuit 1103, the connection between the third chamber 2103 and the third liquid circuit 1103 can be on and off, the third liquid circuit 1103 can be switched to connect to the fourth chamber 2104 or the fourth liquid circuit 1104, and the fourth liquid circuit 1104 is used to discharge the processed sample liquid.
  • the second mixing chamber 2202 when the second mixing chamber 2202 is switched to be connected to the second liquid path 1102, the second mixing chamber 2202 is disconnected from the third liquid path 1103. At this time, if the first mixing chamber 2201 is switched to be connected to the second liquid path 1102, the second mixing chamber 2202 is connected to the first mixing chamber 2201.
  • the second mixing chamber 2202 is switched to communicate with the third liquid path 1103, and the third liquid path 1103 is set to communicate with the third chamber 2103 or the fourth chamber 2104 or the fourth liquid path 1104, then the second mixing chamber 2202 is connected to the third chamber 2103 or the fourth chamber 2104 or the fourth liquid path 1104 accordingly.
  • the first mixing chamber 2201 is provided with a first mixing plunger
  • the second mixing chamber 2202 is provided with a second mixing plunger
  • the first mixing plunger and the second mixing plunger can be pushed and pulled
  • the second chamber 2102 is provided with a second plunger
  • the second plunger can push the washing liquid out of the second chamber 2102
  • the fourth chamber 2104 is provided with a fourth plunger, the fourth plunger can push the eluent out of the fourth chamber 2104.
  • the second plunger, the first mixing plunger, the second mixing plunger and the fourth plunger move automatically.
  • the second plunger, the first mixing plunger, the second mixing plunger and the fourth plunger are driven to move by a plurality of electric telescopic structures, and the operation of each electric telescopic structure is controlled by a host computer.
  • a host computer Of course, it can also be replaced by: driving by hydraulic pressure or pneumatic pressure.
  • the sample extraction card box includes a plurality of sample extraction tubes 3000, the sample extraction tubes 3000 contain reagents for detecting the sample liquid, and the sample extraction tubes 3000 are connected to the card box base 1000.
  • the sample extraction tubes 3000 are connected to the lower side of the card box base 1000, and the lower side of the card box base 1000 is provided with an interface for installing the sample extraction tubes 3000.
  • the sample extraction tube 3000 is connected to the fourth liquid path 1104, and the processed sample liquid is discharged to the sample extraction tube 3000 through the fourth liquid path 1104.
  • the processed sample liquid remains in the second mixing chamber, and the sample liquid is discharged to the sample extraction tube 3000 through the third liquid path 1103 and the fourth liquid path 1104 by pushing the second mixing plunger.
  • the sample liquid is quantitatively discharged, and the quantitative discharge is achieved by controlling the stroke size pushed by the second mixing plunger.
  • the second mixing plunger is equipped with a stroke control element, and the stroke control element can control the moving distance of the second mixing plunger.
  • a magnetic element accommodating area is provided between the first mixing chamber 2201 and the second mixing chamber 2202.
  • the sample extraction cartridge includes a magnetic element, and the magnetic element is close to the magnetic suction chamber of the cartridge base 1000, so that the magnetic beads in the liquid can be adsorbed and fixed.
  • the magnetic element is configured to be close to or away from the magnetic suction chamber of the cartridge base 1000 in the magnetic element accommodating area in a direction perpendicular to the surface of the cartridge base 1000.
  • the sample extraction cartridge does not have a magnetic element, but the user purchases the magnetic element separately, thereby reducing the production cost of the sample extraction cartridge.
  • the sample extraction cartridge includes a plurality of switch valves
  • the cartridge base 1000 is provided with a plurality of recessed installation areas
  • the switch valves are provided in the installation areas
  • each switch valve is used to connect or disconnect the connection between the liquid path assembly and the chamber assembly, and the connection and disconnection at the corresponding position can be switched by switching the position of the switch valve.
  • a flow path is provided in the switch valve, and the flow path forms a first connecting port and a second connecting port on the side of the switch valve.
  • each switch valve is movably connected to the cartridge base 1000, specifically, each switch valve is slidably connected to the cartridge base 1000. Further, each switch valve slides in the cartridge base 1000 in an automatic manner.
  • each switch valve is driven to move by a plurality of electric telescopic structures, and the operation of each electric telescopic structure is controlled by a host computer. Of course, it can also be replaced by: driving by hydraulic pressure or pneumatic pressure.
  • the first chamber 2101 is connected or disconnected with the first liquid path 1101 through the corresponding switch valve switching.
  • the switch valve corresponding to the first chamber 2101 is set as the first switch valve 1201.
  • the first communication port in the first switch valve 1201 is used to connect with the first chamber 2101
  • the second communication port is used to connect with the first liquid path 1101. It can be understood that by changing the position of the first switch valve 1201, the first communication port and the second communication port can be misaligned or aligned with the connection points of the first chamber 2101 and the first liquid path 1101 respectively.
  • the first switch valve 1201 moves to the left, the first chamber 2101 is connected to the first liquid path 1101; if the first switch valve 1201 moves to the right, the first chamber 2101 is disconnected from the first liquid path 1101.
  • the second chamber 2102 is connected or disconnected with the first liquid path 1101 through the corresponding switch valve switching.
  • the switch valve corresponding to the second chamber 2102 is set as the second switch valve 1202.
  • the first communication port in the second switch valve 1202 is used to connect the second chamber 2102, and the second communication port is used to connect the first liquid path 1101. It can be understood that by changing the position of the second switch valve 1202, the first communication port and the second communication port can be misaligned or aligned with the connection points of the second chamber 2102 and the first liquid path 1101 respectively.
  • the second switch valve 1202 moves to the left, the second chamber 2102 is connected to the first liquid path 1101; if the second switch valve 1202 moves to the right, the second chamber 2102 is disconnected from the first liquid path 1101.
  • the first mixing chamber 2201 is switched to communicate with the first liquid path 1101 or the second liquid path 1102 through the corresponding switch valve.
  • the switch valve corresponding to the first mixing chamber 2201 is set as the first mixing switch valve 1301.
  • the first communication port in the first mixing switch valve 1301 is used to communicate with the first mixing chamber 2201, and the second communication port is used to communicate with the first liquid path 1101 or the second liquid path 1102. It can be understood that by changing the position of the first mixing switch valve 1301, the second communication port can be switched to communicate with the first liquid path 1101 or the second liquid path 1102, and the first communication port remains connected with the first mixing chamber 2201.
  • the first mixing switch valve 1301 moves to the left, the first mixing chamber 2201 is connected to the first liquid path 1101; if the first mixing switch valve 1301 moves to the right, the first mixing chamber 2201 is connected to the second liquid path 1102.
  • the second mixing chamber 2202 is switched to communicate with the second liquid path 1102 or the third liquid path 1103 through the corresponding switch valve.
  • the switch valve corresponding to the second mixing chamber 2202 is set as the second mixing switch valve 1302.
  • the first communication port in the second mixing switch valve 1302 is used to communicate with the second mixing chamber 2202, and the second communication port is used to communicate with the second liquid path 1102 or the third liquid path 1103. It can be understood that by changing the position of the second mixing switch valve 1302, the second communication port can be switched to communicate with the second liquid path 1102 or the third liquid path 1103, and the first communication port remains connected with the second mixing chamber 2202.
  • the third chamber 2103 is connected or disconnected with the third liquid path 1103 through the corresponding switch valve switching.
  • the switch valve corresponding to the third chamber 2103 is set as the third switch valve 1203.
  • the first communication port in the third switch valve 1203 is used to connect the third chamber 2103
  • the second communication port is used to connect the third liquid path 1103. It can be understood that by changing the position of the third switch valve 1203, the first communication port and the second communication port can be misaligned or aligned with the connection points of the third chamber 2103 and the third liquid path 1103 respectively.
  • the third switch valve 1203 moves to the left, the third chamber 2103 is connected to the third liquid path 1103; if the third switch valve 1203 moves to the right, the third chamber 2103 is disconnected from the third liquid path 1103.
  • the fourth chamber 2104 can switch the third liquid path 1103 to connect to the fourth chamber 2104 or the third liquid path 1103 to connect to the fourth liquid path 1104 through the corresponding switch valve.
  • the switch valve corresponding to the fourth chamber 2104 is set as the fourth switch valve 1204.
  • the second communication port in the fourth switch valve 1204 is kept in communication with the third liquid path 1103, and the first communication port is used to connect to the fourth chamber 2104.
  • the fourth switch valve 1204 when the first communication port is connected to the fourth chamber 2104 in a positional manner, the second communication port is disconnected from the fourth liquid path 1104 in a dislocated manner, so that the third liquid path 1103 is connected to the fourth chamber 2104; when the first communication port is disconnected from the fourth chamber 2104 in a dislocated manner, the second communication port is connected to the third liquid path 1103 and the fourth liquid path 1104 respectively.
  • the fourth switch valve 1204 moves to the left, the third liquid path 1103 is connected to the fourth chamber 2104 ; when the fourth switch valve 1204 moves to the right, the third liquid path 1103 is connected to the fourth liquid path 1104 .
  • the chamber assembly further includes a fifth chamber 2105, which is connected to the cartridge base 1000, and is connected to the third liquid path 1103, and the connection between the fifth chamber 2105 and the third liquid path 1103 can be switched on and off.
  • the fifth chamber 2105 is used to store mineral oil or gas, and after the processed sample liquid is discharged from the sample extraction cartridge to the sample extraction tube 3000, in order to improve the accuracy of quantitative extraction and prevent residual sample liquid in the liquid path, the sample liquid remaining in the liquid path is pushed out to the sample extraction tube 3000 by using mineral oil or gas.
  • the fifth chamber 2105 is provided with a fifth plunger, and the fifth plunger is used to push out the mineral oil or gas in the fifth chamber 2105.
  • the fifth plunger moves in the fifth chamber 2105 in an automatic manner.
  • the fifth plunger is driven to move by an electric telescopic structure, and the operation of the electric telescopic structure is controlled by a host computer.
  • a host computer Of course, it can also be replaced by: driving by hydraulic pressure or pneumatic pressure.
  • the fifth chamber 2105 is connected or disconnected with the third liquid circuit 1103 through the corresponding switch valve switching.
  • the switch valve corresponding to the fifth chamber 2105 is set to the fifth switch valve 1205.
  • the fifth switch valve 1205 is movably connected to the cartridge base 1000.
  • the fifth switch valve 1205 is slidably connected to the cartridge base 1000.
  • the fifth switch valve 1205 slides in the cartridge base 1000 in an automatic manner.
  • the fifth switch valve 1205 is driven to move by an electric telescopic structure, and the operation of the electric telescopic structure is controlled by a host computer. Of course, it can also be replaced by: driven by hydraulic pressure or pneumatic pressure.
  • the fifth switch valve 1205 is provided with a flow path, and the flow path forms a first communication port and a second communication port on the side of the fifth switch valve 1205, the first communication port is used to connect with the fifth chamber 2105, and the second communication port is used to connect with the third liquid path 1103. It can be understood that by changing the position of the fifth switch valve 1205, the first communication port and the second communication port can be misaligned or aligned with the connection points of the fifth chamber 2105 and the third liquid path 1103, respectively.
  • the switch valve when the chamber combination and the liquid circuit combination are respectively set to multiple, the switch valve is used to synchronously control the on-off of the liquid circuit in each liquid circuit combination, and the same switch valve can simultaneously control the on-off of the same liquid circuit in different liquid circuit combinations.
  • each switch valve is respectively provided with multiple flow paths, and a set of switch valve combinations can simultaneously control multiple independent cartridge structures, realize independent multi-channel sample extraction, and meet the needs of each cartridge structure to independently process the sample liquid.
  • two opposite sides of the cartridge base 1000 are respectively provided with liquid circuit combinations, and the first liquid circuit 1101, the second liquid circuit 1102 and the third liquid circuit 1103 are respectively symmetrically arranged on opposite sides of the cartridge base 1000.
  • the switch valve it is convenient for the switch valve to simultaneously control the on and off of multiple liquid circuit combinations.
  • the sample mixing or flow time in each liquid circuit combination is consistent, which can ensure the transfer amount and mixing degree of each sample to be consistent, and ensure the accuracy of detection.
  • each second connecting port is located on two opposite sides of the switch valve; and on the upper side of the switch valve, each first connecting port is arranged in a row.
  • each chamber corresponding to each switch valve is arranged in a row on the cartridge base 1000.
  • each first chamber 2101, each first mixing chamber 2201 and each second mixing chamber 2202 are arranged in a row on the cartridge base 1000, and correspondingly, each flow path on the first switch valve 1201, the first mixing switch valve 1301 and the second mixing switch valve 1302 are arranged in a row.
  • each first communication port is arranged in two rows, in which case, each chamber corresponding to each switch valve is arranged in two rows on the cartridge base 1000.
  • each second chamber 2102, each third chamber 2103, each fourth chamber 2104 and each fifth chamber 2105 are arranged in two rows on the cartridge base 1000, respectively, and correspondingly, each flow path on the second switch valve 1202, the third switch valve 1203, the fourth switch valve 1204 and the fifth switch valve 1205 are arranged in two rows.
  • each sample extraction tube 3000 is sequentially spaced and distributed along the axial direction of the cartridge base 1000, and the sample extraction tubes 3000 are arranged in a row. In this case, the length of the fourth liquid circuit 1104 corresponding to the sample extraction tube 3000 is not consistent.
  • the mineral oil or gas in the fifth chamber 2105 can be used to discharge the sample liquid in the liquid circuit.
  • the chamber combination and the liquid path combination are respectively set to four to form a quadruple cartridge, and accordingly, the sample extraction tube 3000 is set to four.
  • two opposite sides of the cartridge base 1000 are respectively provided with two liquid path combinations.
  • the four first chambers 2101, the four first mixing chambers 2201 and the four second mixing chambers 2202 are respectively arranged in a row on the cartridge base 1000, and accordingly, the first switching valve 1201, the first mixing switching valve 1301 and the first connecting ports in the second mixing switching valve 1302 are respectively arranged in a row on the upper side.
  • the four second chambers 2102, the four third chambers 2103, the four fourth strong cities and the four fifth chambers 2105 are respectively arranged in two rows on the cartridge base 1000, and accordingly, the first connecting ports in the second switching valve 1202, the third switching valve 1203, the fourth switching valve 1204 and the fifth switching valve 1205 are respectively arranged in two rows on the upper side.
  • a single sample extraction cartridge can process four nucleic acid samples.
  • combining multiple quadruple cartridges can easily realize automated detection under closed conditions of high-throughput cartridges, thereby increasing the number and speed of detection and achieving high-throughput detection.
  • the sample extraction cartridge includes a cover 2300, which is connected to the first chamber 2101 and the connection is detachable. It is understood that in order to maintain the air pressure balance in the first chamber 2101, the first cover 2300 is connected to the gas source, and the gas source is set to the atmosphere or a gas cylinder, and the cover 2300 is set. Further, a filter element is set in the cover 2300 to filter the gas and improve the gas purity.
  • each cover body 2300 is provided in plurality accordingly. Further, each cover body 2300 is connected as one body so as to open and close the cover body 2300 at the same time. In conjunction with the accompanying drawings, each cover body 2300 connected as one body is connected to the sample extraction cartridge. Of course, as an alternative, it can also be designed that each cover body 2300 is connected to each first chamber 2101 in a split manner.
  • the third chamber 2103 is provided with a filter element, and the gas source connected to the third chamber 2103 is the atmosphere or a gas cylinder.
  • the filter element is provided at the open portion of the third chamber 2103 to filter the gas and improve the purity of the gas.
  • nucleic acid detection device In conjunction with the structure of the above-mentioned sample extraction cartridge, the following is a supplementary description of the nucleic acid detection device.
  • the number of sample extraction cartridges is 12
  • the sample extraction cartridges have four chamber combinations and four liquid path combinations
  • the sample extraction tubes 3000 are arranged in a 4*12 array
  • the sample extraction cartridges are arranged in a row in the nucleic acid detection device.
  • the cartridge layout is reasonable, and it is docked with the PCR instrument sample plate, which can realize the detection of 48 independent samples.
  • the number of sample extraction cartridges is set to 24, the sample extraction cartridge has four chamber combinations and four liquid path combinations, the sample extraction tubes 3000 are arranged in a 4*12*2 array, and the sample extraction cartridges are symmetrically arranged in two rows in the nucleic acid detection device. In this case, 98 independent samples can be detected.
  • one overall layout is 8 sample extraction tubes 3000 in horizontal direction and 12 in vertical direction; the other overall layout is 4 sample extraction tubes 3000 in horizontal direction and 24 in vertical direction.
  • the invention relates to a sample extraction method.
  • the sample extraction method uses an automatic method to control the on-off of various liquid paths and chambers in a sample extraction cartridge.
  • the extraction process of the sample extraction method includes magnetic bead activation, sample addition, lysis reaction, washing and elution.
  • the on-off of liquid paths in a plurality of parallel liquid path combinations is synchronously controlled by slidingly connected switch valves to complete the transfer of fluids.
  • the sample extraction method includes the following processes: S1, adding sample solution; S2, lysis mixing, magnetic absorption, and waste liquid discharge; S3, clearing liquid path residues; S4, washing, magnetic absorption, and waste liquid discharge; S5, elution, magnetic absorption; S6, quantitative discharge.
  • step S1 the sample solution to be processed is placed in the first chamber 2101 and pre-mixed in the first mixing chamber 2201 .
  • the throat swab is inserted into the first chamber 2101 , the sample liquid adhered to the throat swab is mixed with the preservation liquid, and the cover body 2300 is covered with the first chamber 2101 .
  • the first switch valve 1201 moves to the left to connect the first chamber 2101 with the first liquid path 1101; the first mixing switch valve 1301 moves to the left to connect the first mixing chamber 2201 with the first liquid path 1101; the sample liquid is transferred to the first mixing chamber 2201 by pushing and pulling the first mixing plunger, and pushing and pulling the first mixing plunger can promote the mixing of the sample liquid and the preservation liquid.
  • step S2 the lysis solution is mixed with the sample solution, the nucleic acid molecules in the sample solution are magnetically adsorbed, and the waste liquid after lysis is transferred to the first chamber 2101 .
  • the first mixing switch valve 1301 moves to the right, and the first mixing chamber 2201 is switched to be connected with the second liquid path 1102; the second mixing switch valve 1302 moves to the left, and the second mixing chamber 2202 is connected with the second liquid path 1102; the sample liquid and the lysis liquid are mixed by pushing and pulling the first mixing plunger and the second mixing plunger, and the sample liquid mixed with the lysis liquid flows back and forth between the first mixing chamber 2201 and the second mixing chamber 2202 to be fully lysed and mixed.
  • the magnetic element is close to the magnetic suction chamber of the cartridge base 1000, and the magnetic element moves downward in the magnetic element accommodating area to the magnetic suction chamber close to the cartridge base 1000; the second mixing plunger is pushed, and the liquid is transferred from the second mixing chamber 2202 to the first mixing chamber 2201.
  • the magnetic element absorbs the magnetic beads, and the magnetic beads are fixed. It can be understood that the mixing speed is reduced during the process of the magnetic element absorbing and fixing the magnetic beads.
  • the first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be connected with the first liquid path 1101; the first switch valve 1201 moves to the left, and the first chamber 2101 is connected with the first liquid path 1101; the waste liquid after lysis is transferred to the first chamber 2101 by pushing the first mixing plunger.
  • step S3 the gas in the third chamber 2103 is used to clean the residual liquid in the first liquid path 1101 and the second liquid path 1102 , and the residual liquid is transferred to the first chamber 2101 .
  • the third switch valve 1203 moves to the left, and the third chamber 2103 is connected to the third liquid path 1103; the second mixing switch valve 1302 moves to the right, and the second mixing chamber 2202 is switched to be connected to the third liquid path 1103; the gas is sucked from the third chamber 2103 to the second mixing chamber 2202 through the second mixing plunger; the third switch valve 1203 moves to the right, and the third chamber 2103 is disconnected from the third liquid path 1103.
  • the second mixing switch valve 1302 moves to the left, and the second mixing chamber 2202 is switched to be connected with the second liquid path 1102; the first mixing switch valve 1301 moves to the right, and the first mixing chamber 2201 is switched to be connected with the second liquid path 1102; by pushing the second mixing plunger, the gas is transferred from the second mixing chamber 2202 to the first mixing chamber 2201, and the gas can clean up the residual liquid in the second liquid path 1102.
  • the first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be connected with the first liquid path 1101; the first switch valve 1201 moves to the left, and the first chamber 2101 is connected with the first liquid path 1101; by pushing the first mixing plunger, the gas enters the first chamber 2101 from the first mixing chamber 2201, and the gas can clean the residual liquid in the first liquid path 1101.
  • step S4 the nucleic acid molecules adsorbed by the magnetic beads are washed, and the waste liquid after washing is transferred to the first chamber 2101 .
  • the second switch valve 1202 moves to the left, and the second chamber 2102 is connected to the first liquid path 1101; the first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be connected to the first liquid path 1101; the first plunger is pushed, and the washing liquid is transferred from the second chamber 2102 to the first mixing chamber 2201 by pulling the first mixing plunger.
  • the first mixing switch valve 1301 moves rightward, and the first mixing chamber 2201 is switched to communicate with the second liquid path 1102; the second mixing switch valve 1302 moves leftward, and the second mixing chamber 2202 is switched to communicate with the second liquid path 1102; the washing liquid flows between the first mixing chamber 2201 and the second mixing chamber 2202 by pushing and pulling the first mixing plunger and the second mixing plunger.
  • the magnetic element is away from the magnetic suction chamber of the cartridge base 1000, and the magnetic beads are suspended in the washing liquid to facilitate the treatment of the nucleic acid molecules by the washing liquid.
  • the magnetic element is close to the magnetic suction chamber of the cartridge base 1000, and the magnetic element moves downward in the magnetic element accommodating area to the magnetic suction chamber of the cartridge base 1000; the second mixing plunger is pushed, and the first mixing plunger is pulled, and the washing liquid is transferred from the second mixing chamber 2202 to the first mixing chamber 2201.
  • the magnetic element absorbs the magnetic beads and the magnetic beads are fixed. It can be understood that the mixing speed is reduced during the process of the magnetic element absorbing and fixing the magnetic beads.
  • the first mixing switch valve 1301 moves to the left, and the first mixing chamber 2201 is switched to be connected with the first liquid path 1101; the first switch valve 1201 moves to the left, and the first chamber 2101 is connected with the first liquid path 1101; by pushing the first mixing plunger, the waste liquid after washing is transferred to the first chamber 2101.
  • step S5 the nucleic acid molecules adsorbed by the magnetic beads are eluted, and the sample liquid obtained by elution is stored in the second mixing chamber 2202.
  • the second mixing switch valve 1302 moves to the right, and the second mixing chamber 2202 is switched to be connected with the third liquid path 1103; the fourth switch valve 1204 moves to the left, and the fourth chamber 2104 is switched to be connected with the third liquid path 1103; the fourth plunger is pushed and the second mixing plunger is pulled, and the eluent is transferred from the fourth chamber 2104 to the second mixing chamber 2202.
  • the second mixing switch valve 1302 moves to the left, and the second mixing chamber 2202 is switched to be connected with the second liquid path 1102; the first mixing switch valve 1301 moves to the right, and the first mixing chamber 2201 is switched to be connected with the second liquid path 1102; the eluent flows between the first mixing chamber 2201 and the second mixing chamber 2202 by pushing and pulling the first mixing plunger and the second mixing plunger.
  • the magnetic element is away from the magnetic suction chamber of the cartridge base 1000, and the magnetic beads are suspended in the sample liquid; after the elution is completed, the magnetic element is close to the magnetic suction chamber of the cartridge base 1000, the magnetic beads are adsorbed and fixed in the magnetic suction chamber, and the sample liquid treated with the elution liquid is transferred to the second mixing chamber 2202.
  • step S6 the sample liquid obtained after elution is quantitatively discharged into the sample extraction tube 3000 , and the sample liquid remaining in the third liquid path 1103 and the fourth liquid path 1104 is cleaned and transferred to the sample extraction tube 3000 .
  • the second mixing switch valve 1302 moves to the right, and the second mixing chamber 2202 is switched to be connected with the third liquid path 1103; the fourth switch valve 1204 moves to the left, and the fourth chamber 2104 is switched to be connected with the third liquid path 1103; the second mixing plunger is pushed to fill the third liquid path 1103 with the sample liquid, so that the residual gas in the third liquid path 1103 is transferred to the fourth chamber 2104.
  • the fourth switch valve 1204 moves rightward, switching the fourth liquid path 1104 to connect with the third liquid path 1103, and extracting a quantitative sample liquid by pushing the second mixing plunger, and the sample liquid is discharged to the sample extraction tube 3000 through the third liquid path 1103 and the fourth liquid path 1104. It can be understood that by setting the moving distance of the second mixing plunger, quantitative liquid discharge can be achieved.
  • the second mixing switch valve 1302 moves to the left, and the second mixing chamber 2202 is switched to be connected with the second liquid circuit 1102, so that the second mixing chamber 2202 is disconnected from the third liquid circuit 1103; the fifth switch valve 1205 moves to the left, and the fifth chamber 2105 is connected with the third liquid circuit 1103; the fifth plunger is pushed, and the mineral oil or gas in the fifth chamber 2105 fills the third liquid circuit 1103 and the fourth liquid circuit 1104, and the sample liquid in the third liquid circuit 1103 and the fourth liquid circuit 1104 is discharged, thereby realizing accurate quantitative liquid extraction.
  • the design can also be replaced by: the magnetic element is close to the magnetic suction chamber of the cartridge base 1000, and during the washing process, the magnetic element maintains the adsorption and fixation of the magnetic beads.

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

一种样品提取卡盒和样品提取方法及核酸检测设备，可广泛应用于生物医药仪器技术领域。样品提取卡盒包括卡盒基座（1000）和多个腔室组合，腔室组合包括第一腔室（2101）、第二腔室（2102）、第一混合腔室（2201）、第二混合腔室（2202）、第三腔室（2103）和第四腔室（2104），第一腔室（2101）用于存放保存液，第二腔室（2102）用于存放洗涤液，第二混合腔室（2202）用于存放裂解液，第三腔室（2103）用于连通气源，第四腔室（2104）用于存放洗脱液；卡盒基座（1000）设置多个液路组合。腔室组合的各腔室与液路组合的各液路对应通断，实现样液的混合、洗涤、清理残液、洗脱及出液，提高提取、检测效率，且废液存放在第一腔室（2101），提高结构利用率。设计样品扩增卡盒具有多个腔室组合和多个液路组合，获得多通道并联的卡盒结构，能够同步处理样液。

Description

样品提取卡盒和样品提取方法及核酸检测设备 技术领域
本发明涉及生物医药仪器技术领域，特别涉及一种样品提取卡盒和样品提取方法及核酸检测设备。
背景技术
在生物分子检测中都会涉及液体之间的转移及特定操作，在转移程中，大多数采用的是开放式的吸取、转移，或在试剂管之间倾倒等方式，容易导致易挥发的有毒试剂挥发扩散到容器外部，污染外部环境，并且会危及操作人员的人身安全。
近年来，快速高通量的检测成为病原体检测的新要求，检测必须做到安全、快速，传统的人工检测和现有自动化检测设备都会不可避免造成使用场景的外部污染，因此需要再特定的进行专业化规划操作。
另外，目前商品化的核酸检测设备虽然也有集提取、样品提取和检测一体的全自动化检测设备，但绝大部分采用单通道卡盒或通过单通道卡盒叠加的形式来完成高通量，难以满足核酸检测设备对高通量的要求，检测效率不高。
发明内容
为解决上述技术问题中的至少之一，本发明提供一种样品提取卡盒和样品提取方法及核酸检测设备，所采用的技术方案如下。
本发明所提供的样品提取卡盒核酸检测设备包括样品提取卡盒。
本发明所提供的样品提取卡盒包括卡盒基座和多个腔室组合，所述腔室组合与所述卡盒基座连接，所述腔室组合包括第一腔室、第二腔室、第一混合腔室、第二混合腔室、第三腔室和第四腔室，所述第一腔室能够用于存放保存液，所述第一腔室与所述卡盒基座连接；所述第二腔室与所述卡盒基座连接，所述第二腔室能够用于存放洗涤液，所述第二腔室设置有第二柱塞；所述第一混合腔室与所述卡盒基座连接，所述第一混合腔室设置有第一混合柱塞；所述第二混合腔室与所述卡盒基座连接，所述第二混合腔室能够用于存放裂解液，所述第二混合腔室设置有第二混合柱塞；所述第三腔室与所述卡盒基座连接，所述第三腔室用于连通气源；所述第四腔室与所述卡盒基座连接，所述第四腔室能够用于存放洗脱液，所述第四腔室设置有第四柱塞；其中，所述卡盒基座设置有多个并联的液路组合，所述液路组合包括第一液路、第二液路、第三液路和第四液路，所述第一液路分别用于连接所述第一腔室、所述第二腔室和所述第一混合腔室，所述第二液路分别用于连接所述第一混合腔室和所述第二混合腔室，所述第三液路分别用于连接所述第二混合腔室、所述第三腔室、所述第四腔室，所述第四液路用于排出处理后的样液，所述液路组合中的各液路可分别通断。
本发明的某些实施例中，所述第一混合腔室和所述第二混合腔室之间设置有磁性元件容置区。
本发明的某些实施例中，所述样品提取卡盒包括磁性元件，所述磁性元件配置为在所述磁性元件容置区可沿垂直于所述卡盒基座表面的方向靠近或远离所述卡盒基座的磁吸室。
本发明的某些实施例中，所述腔室组合还包括第五腔室，所述第五腔室与所述卡盒基座 连接，所述第五腔室用于存放矿物油或气体，所述第五腔室设置有第五柱塞，所述第五腔室连通所述第三液路，且所述第五腔室与所述第三液路的连接可通断。
本发明的某些实施例中，所述样品提取卡盒包括多个开关阀，各所述开关阀分别与所述卡盒基座滑动连接；所述开关阀用于同步控制各所述液路组合中液路的通断。
本发明的某些实施例中，所述卡盒基座的两个相对侧面分别设置有所述液路组合，所述第一液路、所述第二液路、所述第三液路分别在所述卡盒基座的相对两侧对称设置。
本发明的某些实施例中，所述开关阀中设置有多个流路，所述流路在所述开关阀的侧面形成第一连通口和第二连通口，各所述第一连通口在所述开关阀的上侧面排成一列或两列，各所述第二连通口分别位于所述开关阀的相对两个侧面。
本发明的某些实施例中，所述样品提取卡盒包括多个盖体，所述盖体采用分体或一体的方式与所述第一腔室连接。
本发明的某些实施例中，所述盖体中设置有滤芯。
本发明的某些实施例中，所述第三腔室设置有滤芯。
本发明的某些实施例中，所述第二混合柱塞配置行程控制元件，所述行程控制元件控制所述第二混合柱塞的移动距离。
本发明的某些实施例中，所述样品提取卡盒包括四个所述腔室组合和四个所述液路组合。
本发明所提供的样品提取方法使用样品提取卡盒，所述样品提取方法的提取过程包括磁珠活化、加入样本、裂解反应、洗涤和洗脱，通过滑动连接的所述开关阀同步控制多个并联的所述液路组合中液路的通断，完成流体的转移。
本发明可广泛应用于生物医药仪器技术领域，本发明的实施例至少具有以下有益效果：
样品提取卡盒的腔室组合中的各腔室通过与液路组合中的各液路对应通断，在密闭环境中连续实现样液的混合、洗涤、清理残液、洗脱及出液，提高提取、检测效率，并且所产生的废液能够存放在第一腔室中，提高结构利用率。
设计样品提取卡盒具有多个腔室组合和多个液路组合，且各液路组合之间并联，从而获得多通道并联的卡盒结构，防止串流，能够同步处理多份样液，进一步提高效率。
卡盒基座中设计开关阀，开关阀能够滑动变换位置，从而控制液路组合中各液路之间的通断，且开关阀能够同时控制各液路组合中的液路，操作更加便利。
在卡盒基座的相对两侧对称设置液路组合，相互独立，防止串液，且各液路组合中样液的混合或流动的时间一致，确保检测的准确性。
附图说明
本发明的实施例所描述和/或所附加的方面和优点，结合下面附图将变得明显和容易理解。应说明的是，下面附图所体现的实施例是示例性的，仅用于解释本发明，而不能理解为对本发明的限制。
图1为样品提取卡盒的一种实施例结构。
图2为图1中样品提取卡盒的结构图，图中显示各开关阀从卡盒基座分解，腔室组合为四个。
图3为12个样品提取卡盒按照4*12的布局排列。
图4为24个样品提取卡盒按照4*12*2的布局对称排成两排，图中显示为横4、纵24。
图5为24个样品提取卡盒按照4*12*2的布局对称排成两排，图中显示为横8、纵12。
附图标记：
1000、卡盒基座；
1101、第一液路；1102、第二液路；1103、第三液路；1104、第四液路；
1201、第一开关阀；1202、第二开关阀；1203、第三开关阀；1204、第四开关阀；1205、第五开关阀；
1301、第一混合开关阀；1302、第二混合开关阀；
2101、第一腔室；2102、第二腔室；2103、第三腔室；2104、第四腔室；2105、第五腔室；
2201、第一混合腔室；2202、第二混合腔室；
2300、盖体；
3000、样品提取管。
具体实施方式
下面结合图1至图5详细描述本发明的实施例，所述实施例的示例在附图中示出，其中自始至终相同或类似的标号表示相同或类似的元件或具有相同或类似功能的元件。下面通过参考附图描述的实施例是示例性的，仅用于解释本发明，而不能理解为对本发明的限制。
在本发明的描述中，需要理解的是，若出现术语“中心”、“中部”、“纵向”、“横向”、“长度”、“宽度”、“厚度”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”、“轴向”、“径向”、“周向”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本发明和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。此外，限定有“第一”、“第二”的特征可以明示或者隐含地包括一个或者更多个该特征。在本发明的描述中，除非另有说明，“多个”的含义是两个或两个以上。
在本发明的描述中，需要说明的是，除非另有明确的规定和限定，术语“安装”、“相连”、“连接”应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或一体地连接；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通。对于本领域的普通技术人员而言，可以具体情况理解上述术语在本发明中的具体含义。
本发明涉及一种核酸检测设备，核酸检测设备包括样品提取卡盒，核酸的样液在样品提取卡盒中经裂解、洗涤和洗脱后排出，以便检测。
核酸检测设备的其他构成以及操作对于本领域普通技术人员而言在相关技术中已有记载，这里不再详细描述，以下将对样品提取卡盒的结构展开介绍。
本发明涉及一种样品提取卡盒，样品提取卡盒包括卡盒基座1000及多个腔室组合，腔室组合包括第一腔室2101、第二腔室2102、第一混合腔室2201、第二混合腔室2202、第三腔室2103和第四腔室2104，具体地，第一腔室2101、第二腔室2102、第一混合腔室2201、第二混合腔室2202、第三腔室2103以及第四腔室2104分别与卡盒基座1000连接。进一步地，第一腔室2101能够用于存放保存液，粘有样液的咽拭子放入第一腔室2101并与保存液混合， 第二腔室2102能够用于存放洗涤液，第二混合腔室2202能够用于存放裂解液，裂解液中混有磁珠，第三腔室2103用于连通气源，第四腔室2104能够用于存放洗脱液。
卡盒基座1000设置有多个液路组合，液路组合用于实现腔室组合中各腔室之间的连通，具体地，液路组合包括第一液路1101、第二液路1102、第三液路1103和第四液路1104，各液路通过微纳加工形成，均为微米级别的液路，且液路组合中的各液路能够分别通断，以实现不同腔室之间的连通。进一步地，各液路组合之间并联，各腔室组合通过对应的液路组合构成并联式的、相互独立的卡盒结构，结构紧凑，相互之间不串液，能够同时处理多份核酸的样液，提高效率。
第一液路1101分别用于连接第一腔室2101、第二腔室2102和第一混合腔室2201，具体地，第一腔室2101和第二腔室2102与第一液路1101的连接可分别通断，第二液路1102分别用于连接第一混合腔室2201和第二混合腔室2202，第一混合腔室2201可切换连通第一液路1101或第二液路1102。可以理解的是，若第一混合腔室2201切换至与第二液路1102连通，则第一混合腔室2201与第一液路1101断开。
当然，在第一混合腔室2201切换至与第一液路1101连通的情况下，则第一混合腔室2201与第二液路1102断开，此时：若第一腔室2101与第一液路1101连通且第二腔室2102与第一液路1101断开，则第一腔室2101和第一混合腔室2201连通，液体可在第一腔室2101和第一混合腔室2201之间流动；若第二腔室2102与第一液路1101连通且第一腔室2101与第一液路1101，则第二腔室2102与第一混合腔室2201连通，液体可在第二腔室2102和第一混合腔室2201之间流动。
第三液路1103分别用于连接第二混合腔室2202、第三腔室2103、第四腔室2104和第四液路1104，具体地，第二混合腔室2202可切换连通第二液路1102或第三液路1103，第三腔室2103与第三液路1103的连接可通断，第三液路1103可切换连通第四腔室2104或第四液路1104，第四液路1104用于排出处理后的样液。
可以理解的是，在第二混合腔室2202切换至与第二液路1102连通的情况下，第二混合腔室2202与第三液路1103断开，此时若第一混合腔室2201切换至与第二液路1102连通，则第二混合腔室2202与第一混合腔室2201连通。
当然，若第二混合腔室2202切换至与第三液路1103连通，设定第三液路1103连通第三腔室2103或第四腔室2104或第四液路1104，则相应地，第二混合腔室2202连通第三腔室2103或第四腔室2104或第四液路1104。
进一步地，第一混合腔室2201设置有第一混合柱塞，第二混合腔室2202设置有第二混合柱塞，第一混合柱塞和第二混合柱塞可推拉，第二腔室2102设置有第二柱塞，第二柱塞可将洗涤液从第二腔室2102中推出，第四腔室2104设置有第四柱塞，第四柱塞可将洗脱液从第四腔室2104中推出。
一些示例中，第二柱塞、第一混合柱塞、第二混合柱塞和第四柱塞以自动的方式移动。具体地，通过多个电动伸缩结构分别驱使第二柱塞、第一混合柱塞、第二混合柱塞和第四柱塞移动，利用上位机分别控制各电动伸缩结构的工作。当然，也可替换为：以液压或气压的方式驱动。
可以理解的是，对应于腔室组合和液路组合，样品提取卡盒包括多个样品提取管3000， 样品提取管3000中具有用于检测样液的试剂，样品提取管3000与卡盒基座1000连接，具体地，样品提取管3000连接卡盒基座1000的下侧面，卡盒基座1000的下侧面设置有用于安装样品提取管3000的接口。
进一步地，样品提取管3000与第四液路1104连通，处理后的样液经第四液路1104排放至样品提取管3000。具体地，处理后的样液留存在第二混合腔室中，通过推动第二混合柱塞将样液经第三液路1103和第四液路1104排至样品提取管3000。可以理解的是，样液为定量排出，通过控制第二混合柱塞推动的行程大小实现定量排出。一些示例中，第二混合柱塞配置行程控制元件，行程控制元件能够控制第二混合柱塞的移动距离。
作为一种实施方式，第一混合腔室2201和第二混合腔室2202之间设置有磁性元件容置区。进一步地，样品提取卡盒包括磁性元件，磁性元件靠近卡盒基座1000的磁吸室，则可将液体中的磁珠吸附固定。具体地，磁性元件配置为在磁性元件容置区可沿垂直于卡盒基座1000表面的方向靠近或远离卡盒基座1000的磁吸室。
当然，作为替换方案，还可设计为：样品提取卡盒不具有磁性元件，而是使用者另外购置磁性元件，减少样品提取卡盒的生产成本。
作为一种实施方式，样品提取卡盒包括多个开关阀，卡盒基座1000设置有多个凹陷的安装区，开关阀设置在安装区，各开关阀分别用于连通或切断液路组合与腔室组合之间的各处连接，通过切换开关阀的位置可切换上述对应位置处的通断。具体地，开关阀中设置有流路，流路在开关阀的侧面形成第一连通口和第二连通口。
可以理解的是，各开关阀分别与卡盒基座1000活动连接，具体地，各开关阀分别与卡盒基座1000滑动连接。进一步地，各开关阀以自动的方式在卡盒基座1000中滑动。一些示例中，通过多个电动伸缩结构分别驱使各开关阀移动，利用上位机分别控制各电动伸缩结构的工作。当然，也可替换为：以液压或气压的方式驱动。
第一腔室2101通过对应的开关阀切换与第一液路1101连通或断开，第一腔室2101所对应的开关阀设置为第一开关阀1201，第一开关阀1201中的第一连通口用于连通第一腔室2101、第二连通口用于连通第一液路1101。可以理解的是，变换第一开关阀1201的位置，则可使第一连通口、第二连通口分别与第一腔室2101、第一液路1101的连通处错位或对位。结合附图，第一开关阀1201左移，则第一腔室2101连通第一液路1101；第一开关阀1201右移，则第一腔室2101与第一液路1101断开。
第二腔室2102通过对应的开关阀切换与第一液路1101连通或断开，第二腔室2102所对应的开关阀设置为第二开关阀1202，第二开关阀1202中的第一连通口用于连通第二腔室2102、第二连通口用于连通第一液路1101。可以理解的是，变换第二开关阀1202的位置，则可使第一连通口、第二连通口分别与第二腔室2102、第一液路1101的连通处错位或对位。结合附图，第二开关阀1202左移，则第二腔室2102连通第一液路1101；第二开关阀1202右移，则第二腔室2102与第一液路1101断开。
第一混合腔室2201通过对应的开关阀切换与第一液路1101或第二液路1102连通，第一混合腔室2201所对应的开关阀设置为第一混合开关阀1301，第一混合开关阀1301中的第一连通口用于连通第一混合腔室2201、第二连通口用于连通第一液路1101或第二液路1102。可以理解的是，变换第一混合开关阀1301的位置，则可使第二连通口切换连通第一液路1101 或第二液路1102，且第一连通口保持与第一混合腔室2201连通。结合附图，第一混合开关阀1301左移，则第一混合腔室2201连通第一液路1101；第一混合开关阀1301右移，则第一混合腔室2201连通第二液路1102。
第二混合腔室2202通过对应的开关阀切换与第二液路1102或第三液路1103连通，第二混合腔室2202所对应的开关阀设置为第二混合开关阀1302，第二混合开关阀1302中的第一连通口用于连通第二混合腔室2202、第二连通口用于连通第二液路1102或第三液路1103。可以理解的是，变换第二混合开关阀1302的位置，则可使第二连通口切换连通第二液路1102或第三液路1103，且第一连通口保持与第二混合腔室2202连通。结合附图，第二混合开关阀1302左移，则第二混合腔室2202连通第二液路1102；第二混合开关阀1302右移，则第二混合腔室2202连通第三液路1103。
第三腔室2103通过对应的开关阀切换与第三液路1103连通或断开，第三腔室2103所对应的开关阀设置为第三开关阀1203，第三开关阀1203中的第一连通口用于连通第三腔室2103、第二连通口用于连通第三液路1103。可以理解的是，变换第三开关阀1203的位置，则可使第一连通口、第二连通口分别与第三腔室2103、第三液路1103的连通处错位或对位。结合附图，第三开关阀1203左移，则第三腔室2103连通第三液路1103；第三开关阀1203右移，则第三腔室2103与第三液路1103断开。
第四腔室2104通过对应的开关阀能够切换第三液路1103连通第四腔室2104或者第三液路1103连通第四液路1104，第四腔室2104所对应的开关阀设置为第四开关阀1204，第四开关阀1204中的第二连通口与第三液路1103保持连通，第一连通口用于连通第四腔室2104。可以理解的是，变换第四开关阀1204的位置，在第一连通口与第四腔室2104对位连通的情况下，则第二连通口与第四液路1104错位断开，从而第三液路1103与第四腔室2104连通；在第一连通口与第四腔室2104错位断开的情况下，第二连通口分别连通第三液路1103和第四液路1104。结合附图，第四开关阀1204左移，则第三液路1103连通第四腔室2104；第四开关阀1204右移，则第三液路1103连通第四液路1104。
作为一种实施方式，腔室组合还包括第五腔室2105，第五腔室2105与卡盒基座1000连接，第五腔室2105连通第三液路1103，且第五腔室2105与第三液路1103的连接可通断。第五腔室2105用于存放矿物油或气体，处理后的样液从样品提取卡盒排出至样品提取管3000后，为提高定量提取的精准度和防止液路中残留样液，利用矿物油或气体将液路中残留的样液推出至样品提取管3000。
进一步地，第五腔室2105设置有第五柱塞，第五柱塞用于将第五腔室2105中的矿物油或气体推出。一些示例中，第五柱塞以自动的方式在第五腔室2105中移动。具体地，通过电动伸缩结构驱使第五柱塞移动，利用上位机控制电动伸缩结构的工作。当然，也可替换为：以液压或气压的方式驱动。
第五腔室2105通过对应的开关阀切换与第三液路1103连通或断开，具体地，第五腔室2105所对应的开关阀设置为第五开关阀1205。可以理解的是，第五开关阀1205与卡盒基座1000活动连接。具体地，第五开关阀1205与卡盒基座1000滑动连接。进一步地，第五开关阀1205以自动的方式在卡盒基座1000中滑动。一些示例中，通过电动伸缩结构驱使第五开关阀1205移动，利用上位机控制电动伸缩结构的工作。当然，也可替换为：以液压或气压的 方式驱动。
第五开关阀1205中设置有流路，流路在第五开关阀1205的侧面形成第一连通口和第二连通口，第一连通口用于连通第五腔室2105、第二连通口用于连通第三液路1103。可以理解的是，变换第五开关阀1205的位置，则可使第一连通口、第二连通口分别与第五腔室2105、第三液路1103的连通处错位或对位。
可以理解的是，在腔室组合、液路组合分别设置为多个的情况下，开关阀用于同步控制各液路组合中液路的通断，且同一个开关阀能够同时控制不同液路组合中相同液路的通断。具体地，各开关阀分别设置有多个流路，利用一套开关阀组合能够同时控制多个独立的卡盒结构，实现独立的多通道样品提取，满足各卡盒结构独立处理样液的需求。
一些示例中，卡盒基座1000的两个相对侧面分别设置有液路组合，第一液路1101、第二液路1102和第三液路1103分别在卡盒基座1000的相对两侧对称设置，一方面，方便开关阀同时控制多个液路组合的通断，另一方面，每个液路组合中的样本混合或流动的时间一致，能够确保各样本的转移量、混匀程度一致，确保检测准确性。可以理解的是，开关阀上，各第二连通口位于开关阀的两个相对侧面；且在开关阀的上侧面，各第一连通口排成一列。这种情况下，各开关阀所对应的各腔室在卡盒基座1000上排成一列。具体地，各第一腔室2101、各第一混合腔室2201和各第二混合腔室2202在卡盒基座1000上分别排成一列，相应地，第一开关阀1201、第一混合开关阀1301、第二混合开关阀1302上的各流路分别排成一列。
当然，作为替换方案，还可设计为：在开关阀的上侧面，各第一连通口排成两列，这种情况下，各开关阀所对应的各腔室在卡盒基座1000上排成两列。具体地，各第二腔室2102、各第三腔室2103、各第四腔室2104和各第五腔室2105在卡盒基座1000上分别排成两列，相应地，第二开关阀1202、第三开关阀1203、第四开关阀1204和第五开关阀1205上的各流路分别排成两列。
可以理解的是，样品提取管3000的数量与腔室组合和液路组合的数量一致。进一步地，各样品提取管3000沿卡盒基座1000的轴向依次间隔分布，样品提取管3000排成一列。这种情况下，样品提取管3000所对应连接的第四液路1104则长度并不一致，为消除取液的定量偏差，可利用第五腔室2105中的矿物油或气体将液路中的样液排出。
结合附图，腔室组合和液路组合分别设置为四个，构成四联体卡盒，相应地，样品提取管3000设置为四个。进一步地，卡盒基座1000的两个相对侧面分别设置两个液路组合。具体地，四个第一腔室2101、四个第一混合腔室2201和四个第二混合腔室2202在卡盒基座1000上分别排成一列，相应地，第一开关阀1201、第一混合开关阀1301和第二混合开关阀1302中的各第一连通口在上侧面分别排成一列。四个第二腔室2102、四个第三腔室2103、四个第四强市和四个第五腔室2105在卡盒基座1000上分别排成两列，相应地，第二开关阀1202、第三开关阀1203、第四开关阀1204和第五开关阀1205中的各第一连通口在上侧面分别排成两列。
可以理解的是，单个样品提取卡盒能够处理四个核酸样本，在核酸检测设备中，组合多个四联体卡盒，能够很方便地实现高通量卡盒封闭条件下的自动化检测，提高检测数量和检测速度，实现高通量检测。
作为一种实施方式，样品提取卡盒包括盖体2300，盖体2300与第一腔室2101连接，且 连接可分离。可以理解的是，为维持第一腔室2101中的气压平衡，第一盖体2300连通气源，气源设置为大气或气瓶，盖体2300设置。进一步地，盖体2300中设置有滤芯，以便过滤气体，提高气体纯度。
可以理解的是，在第一腔室2101设置为多个的情况下，相应地，盖体2300设置为多个。进一步地，各盖体2300连成一体，以便同时打开和闭合盖体2300。结合附图，连为一体的各盖体2300与样品提取卡盒连接。当然，作为替换方案，还可设计为：各盖体2300采用分体的方式分别与各第一腔室2101连接。
作为一种实施方式，第三腔室2103设置有滤芯，第三腔室2103所连接的气源为大气或气瓶，在第三腔室2103的敞口处设置滤芯可过滤气体，提高气体纯度。
结合上述样品提取卡盒的结构，以下对核酸检测设备的补充说明。
作为一种实施方式，样品提取卡盒的数量为12个，样品提取卡盒具有四个腔室组合及四个液路组合，样品提取管3000按照4*12的阵列分布布局，并将各样品提取卡盒排成一排设置在核酸检测设备中。这种情况下，卡盒布局合理，与PCR仪样品板对接，能够实现48个独立样本的检测。
作为一种实施方式，样品提取卡盒的数量设置为24个，样品提取卡盒具有四个腔室组合及四个液路组合，样品提取管3000按照4*12*2的阵列分布布局，并将各样品提取卡盒对称排成两排设置在核酸检测设备中。这种情况下，能够实现98个独立样本的检测。
对称设置的方式有两种：一种整体布局为样品提取管3000横8个、纵12个；另一种整体布局为样品提取管3000横4个、纵24个。
进一步地，若按上述任一种对称方式再增设24个样品提取卡盒，则能够实现192个独立样本的检测。
根据上述对样品提取卡盒结构的详细描述，下面对采用样品提取卡盒处理样液的样品提取方法展开介绍，应注意的是，下述描述为示例性说明，而不是对发明的具体限制。
本发明涉及一种样品提取方法，样品提取方法采用自动的方式控制样品提取卡盒中各处液路和腔室的通断，样品提取方法的提取过程包括磁珠活化、加入样本、裂解反应、洗涤和洗脱，通过滑动连接的开关阀同步控制多个并联的液路组合中液路的通断，完成流体的转移。
样品提取方法包括如下流程：S1，添加样液；S2，裂解混合，磁吸，排废液；S3，清除液路残留；S4，洗涤,磁吸，排废液；S5，洗脱，磁吸；S6，定量排出。
步骤S1中待处理样液放入第一腔室2101并在第一混合腔室2201预混合。
咽拭子插入第一腔室2101，咽拭子所粘的样液与保存液混合，盖体2300与第一腔室2101盖合。
第一开关阀1201左移，以使第一腔室2101与第一液路1101连通；第一混合开关阀1301左移，以使第一混合腔室2201与第一液路1101连通；通过推拉第一混合柱塞将样液转移至第一混合腔室2201，推拉第一混合柱塞能够促进样液和保存液混合。
步骤S2中，裂解液与样液混合，对样液中的核酸分子磁性吸附，并将裂解后的废液转移至第一腔室2101。
第一混合开关阀1301右移，第一混合腔室2201切换至与第二液路1102连通；第二混合开关阀1302左移，第二混合腔室2202与第二液路1102连通；通过推拉第一混合柱塞和第二 混合柱塞将样液和裂解液混合，样液混合裂解液在第一混合腔室2201和第二混合腔室2202之间往复流动，充分裂解混合。
磁性元件靠近卡盒基座1000的磁吸室，磁性元件在磁性元件容置区向下移动靠近卡盒基座1000的磁吸室；推动第二混合柱塞，液体从第二混合腔室2202转移至第一混合腔室2201，样液流动过程中，磁性元件吸附磁珠，磁珠固定。可以理解的是，磁性元件吸附固定磁珠的过程中，降低混合速度。
第一混合开关阀1301左移，第一混合腔室2201切换至与第一液路1101连通；第一开关阀1201左移，第一腔室2101与第一液路1101连通；通过推动第一混合柱塞将裂解后的废液转移至第一腔室2101。
步骤S3中，利用第三腔室2103中的气体清理第一液路1101和第二液路1102中的残液，并将残液转移至第一腔室2101。
第三开关阀1203左移，第三腔室2103连通第三液路1103；第二混合开关阀1302右移，第二混合腔室2202切换至与第三液路1103连通；通过第二混合柱塞将气体从第三腔室2103吸引至第二混合腔室2202；第三开关阀1203右移，第三腔室2103与第三液路1103断开。
第二混合开关阀1302左移，第二混合腔室2202切换至与第二液路1102连通；第一混合开关阀1301右移，第一混合腔室2201切换至与第二液路1102连通；通过推动第二混合柱塞，将气体从第二混合腔室2202转移至第一混合腔室2201，气体能够清理第二液路1102中的残液。
第一混合开关阀1301左移，第一混合腔室2201切换至与第一液路1101连通；第一开关阀1201左移，第一腔室2101与第一液路1101连通；通过推动第一混合柱塞，将气体从第一混合腔室2201进入第一腔室2101，气体能够清理第一液路1101中的残液。
步骤S4中，对磁珠所吸附的核酸分子洗涤，并将洗涤后的废液转移至第一腔室2101。
第二开关阀1202左移，第二腔室2102连通第一液路1101；第一混合开关阀1301左移，第一混合腔室2201切换至与第一液路1101连通；推动第一柱塞，并通过拉动第一混合柱塞将洗涤液从第二腔室2102转移至第一混合腔室2201。
第一混合开关阀1301右移，第一混合腔室2201切换至与第二液路1102连通；第二混合开关阀1302左移，第二混合腔室2202切换至与第二液路1102连通；通过推拉第一混合柱塞和第二混合柱塞以使洗涤液在第一混合腔室2201和第二混合腔室2202之间流动。可以理解的是，此过程中，磁性元件远离卡盒基座1000的磁吸室，磁珠在洗涤液中悬浮，以促进洗涤液对核酸分子的处理。
磁性元件靠近卡盒基座1000的磁吸室，磁性元件在磁性元件容置区向下移动靠近卡盒基座1000的磁吸室；推动第二混合柱塞，并拉动第一混合柱塞，洗涤液从第二混合腔室2202转移至第一混合腔室2201，液体流动过程中，磁性元件吸附磁珠，磁珠固定。可以理解的是，磁性元件吸附固定磁珠的过程中，降低混合速度。
第一混合开关阀1301左移，第一混合腔室2201切换至与第一液路1101连通；第一开关阀1201左移，第一腔室2101连通第一液路1101；通过推动第一混合柱塞，将洗涤后的废液转移至第一腔室2101。
步骤S5中，对磁珠所吸附的核酸分子进行洗脱，并将洗脱所获得的样液存放在第二混合 腔室2202。
第二混合开关阀1302右移，第二混合腔室2202切换至与第三液路1103连通；第四开关阀1204左移，切换第四腔室2104连通第三液路1103；推动第四柱塞，并拉动第二混合柱塞，洗脱液从第四腔室2104转移至第二混合腔室2202。
第二混合开关阀1302左移，第二混合腔室2202切换至与第二液路1102连通；第一混合开关阀1301右移，第一混合腔室2201切换至与第二液路1102连通；通过推拉第一混合柱塞和第二混合柱塞以使洗脱液在第一混合腔室2201和第二混合腔室2202之间流动。
洗脱过程中，磁性元件远离卡盒基座1000的磁吸室，磁珠悬浮在样液中；洗脱完成后，磁性元件靠近卡盒基座1000的磁吸室，将磁珠吸附固定在磁吸室，并将洗脱液处理后的样液转移至第二混合腔室2202。
步骤S6中，洗脱后获得的样液定量排出至样品提取管3000，并将残留在第三液路1103和第四液路1104中的样液清理至样品提取管3000。
第二混合开关阀1302右移，第二混合腔室2202切换至与第三液路1103连通；第四开关阀1204左移，切换第四腔室2104连通第三液路1103；通过推动第二混合柱塞以使样液充满第三液路1103，以使第三液路1103中残留的气体转移至第四腔室2104。
第四开关阀1204右移，切换第四液路1104连通第三液路1103，通过推动第二混合柱塞提取定量的样液，样液经第三液路1103和第四液路1104排出至样品提取管3000。可以理解的是，设定第二混合柱塞的移动距离，则可实现定量排液。
第二混合开关阀1302左移，第二混合腔室2202切换至与第二液路1102连通，以使第二混合腔室2202与第三液路1103断开；第五开关阀1205左移，第五腔室2105连通第三液路1103；推动第五柱塞，第五腔室2105中的矿物油或气体充满第三液路1103和第四液路1104，第三液路1103和第四液路1104中的样液排出，实现精确定量取液。
可以理解的是，在步骤S4洗涤的过程中，还可替换设计为：磁性元件靠近卡盒基座1000的磁吸室，在洗涤过程中，磁性元件保持对磁珠的吸附固定。
在本说明书的描述中，若出现参考术语“一个实施例”、“一些实例”、“一些实施例”、“示意性实施例”、“示例”、“具体示例”、或“一些示例”等的描述意指结合该实施例或示例描述的具体特征、结构、材料或者特点包含于本发明的至少一个实施例或示例中。在本说明书中，对上述术语的示意性表述不一定指的是相同的实施例或示例。而且，描述的具体特征、结构、材料或者特点可以在任何的一个或多个实施例或示例中以合适的方式结合。
以上结合附图对本发明的实施方式作了详细说明，但是本发明不限于上述实施方式，在所述技术领域普通技术人员所具备的知识范围内，还可以在不脱离本发明宗旨的前提下作出各种变化。
本发明的描述中，专利名称若出现“、”，表示“和”的关系，而不是“或”的关系。例如专利名称为“一种A、B”，说明本发明所要求保护的内容为：主题名称为A的技术方案和主题名称为B的技术方案。

Claims (14)

  1. 一种样品提取卡盒，其特征在于：包括卡盒基座(1000)和多个腔室组合，所述腔室组合与所述卡盒基座(1000)连接，所述腔室组合包括：
    第一腔室(2101)，所述第一腔室(2101)用于存放保存液，所述第一腔室(2101)与所述卡盒基座(1000)连接；
    第二腔室(2102)，所述第二腔室(2102)与所述卡盒基座(1000)连接，所述第二腔室(2102)用于存放洗涤液，所述第二腔室(2102)设置有第二柱塞；
    第一混合腔室(2201)，所述第一混合腔室(2201)与所述卡盒基座(1000)连接，所述第一混合腔室(2201)设置有第一混合柱塞；
    第二混合腔室(2202)，所述第二混合腔室(2202)与所述卡盒基座(1000)连接，所述第二混合腔室(2202)用于存放裂解液，所述第二混合腔室(2202)设置有第二混合柱塞；
    第三腔室(2103)，所述第三腔室(2103)与所述卡盒基座(1000)连接，所述第三腔室(2103)用于连通气源；
    第四腔室(2104)，所述第四腔室(2104)与所述卡盒基座(1000)连接，所述第四腔室(2104)用于存放洗脱液，所述第四腔室(2104)设置有第四柱塞；
    其中，所述卡盒基座(1000)设置有多个并联的液路组合，所述液路组合包括第一液路(1101)、第二液路(1102)、第三液路(1103)和第四液路(1104)，所述第一液路(1101)分别用于连接所述第一腔室(2101)、所述第二腔室(2102)和所述第一混合腔室(2201)，所述第二液路(1102)分别用于连接所述第一混合腔室(2201)和所述第二混合腔室(2202)，所述第三液路(1103)分别用于连接所述第二混合腔室(2202)、所述第三腔室(2103)、所述第四腔室(2104)，所述第四液路(1104)用于排出处理后的样液，所述液路组合中的各液路可分别通断。
  2. 根据权利要求1所述的样品提取卡盒，其特征在于：所述第一混合腔室(2201)和所述第二混合腔室(2202)之间设置有磁性元件容置区。
  3. 根据权利要求2所述的样品提取卡盒，其特征在于：所述样品提取卡盒包括磁性元件，所述磁性元件配置为在所述磁性元件容置区可沿垂直于所述卡盒基座(1000)表面的方向靠近或远离所述卡盒基座(1000)的磁吸室。
  4. 根据权利要求3所述的样品提取卡盒，其特征在于：所述腔室组合还包括第五腔室(2105)，所述第五腔室(2105)与所述卡盒基座(1000)连接，所述第五腔室(2105)用于存放矿物油或气体，所述第五腔室(2105)设置有第五柱塞，所述第五腔室(2105)连通所述第三液路(1103)，且所述第五腔室(2105)与所述第三液路(1103)的连接可通断。
  5. 根据权利要求4所述的样品提取卡盒，其特征在于：所述样品提取卡盒包括多个开关阀，各所述开关阀分别与所述卡盒基座(1000)滑动连接；所述开关阀用于同步控制各所述液路组合中液路的通断。
  6. 根据权利要求5所述的样品提取卡盒，其特征在于：所述卡盒基座(1000)的两个相对侧面分别设置有所述液路组合，所述第一液路(1101)、所述第二液路(1102)、所述第三液路(1103)分别在所述卡盒基座(1000)的相对两侧对称设置。
  7. 根据权利要求6所述的样品提取卡盒，其特征在于：所述开关阀中设置有多个流路，所述流路在所述开关阀的侧面形成第一连通口和第二连通口，各所述第一连通口在所述开关阀的上侧面排成一列或两列，各所述第二连通口分别位于所述开关阀的相对两个侧面。
  8. 根据权利要求1所述的样品提取卡盒，其特征在于：所述样品提取卡盒包括多个盖体(2300)，所述盖体(2300)采用分体或一体的方式与所述第一腔室(2101)连接。
  9. 根据权利要求8所述的样品提取卡盒，其特征在于：所述盖体(2300)中设置有滤芯。
  10. 根据权利要求1至9任一项所述的样品提取卡盒，其特征在于：所述第三腔室(2103)设置有滤芯。
  11. 根据权利要求1至7任一项所述的样品提取卡盒，其特征在于：所述第二混合柱塞配置行程控制元件，所述行程控制元件控制所述第二混合柱塞的移动距离。
  12. 根据权利要求4至7任一项所述的样品提取卡盒，其特征在于：所述样品提取卡盒包括四个所述腔室组合和四个所述液路组合。
  13. 一种样品提取方法，其特征在于：所述样品提取方法使用如权利要求5至7任一项所述的样品提取卡盒，所述样品提取方法的提取过程包括磁珠活化、加入样本、裂解反应、洗涤和洗脱，通过滑动连接的所述开关阀同步控制多个并联的所述液路组合中液路的通断，完成流体的转移。
  14. 一种核酸检测设备，其特征在于：包括如权利要求1至12任一项所述的样品提取卡盒。
PCT/CN2022/132302 2022-11-16 2022-11-16 样品提取卡盒和样品提取方法及核酸检测设备 WO2024103297A1 (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2022/132302 WO2024103297A1 (zh) 2022-11-16 2022-11-16 样品提取卡盒和样品提取方法及核酸检测设备

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2022/132302 WO2024103297A1 (zh) 2022-11-16 2022-11-16 样品提取卡盒和样品提取方法及核酸检测设备

Publications (1)

Publication Number Publication Date
WO2024103297A1 true WO2024103297A1 (zh) 2024-05-23

Family

ID=91083413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2022/132302 WO2024103297A1 (zh) 2022-11-16 2022-11-16 样品提取卡盒和样品提取方法及核酸检测设备

Country Status (1)

Country Link
WO (1) WO2024103297A1 (zh)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112538414A (zh) * 2020-12-08 2021-03-23 杭州奥盛仪器有限公司 一种设有微流控结构的全密封式核酸智能化提取装置
CN113684118A (zh) * 2021-07-14 2021-11-23 浙江大学 一种集成核酸分析芯片
WO2021237396A1 (zh) * 2020-05-25 2021-12-02 杭州梓晶生物有限公司 一种一体化自助式核酸检测装置及其使用方法
WO2022106423A1 (en) * 2020-11-19 2022-05-27 Ttp Plc Apparatus
CN114558632A (zh) * 2022-04-26 2022-05-31 广州国家实验室 液体转移装置、多通道液体转移装置以及方法
CN114574349A (zh) * 2022-04-26 2022-06-03 广州国家实验室 一种适配卡盒使用的设备
CN114657049A (zh) * 2022-05-12 2022-06-24 广州国家实验室 一种核酸扩增用卡盒
CN217578917U (zh) * 2022-04-26 2022-10-14 广州国家实验室 样本提取装置

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021237396A1 (zh) * 2020-05-25 2021-12-02 杭州梓晶生物有限公司 一种一体化自助式核酸检测装置及其使用方法
WO2022106423A1 (en) * 2020-11-19 2022-05-27 Ttp Plc Apparatus
CN112538414A (zh) * 2020-12-08 2021-03-23 杭州奥盛仪器有限公司 一种设有微流控结构的全密封式核酸智能化提取装置
CN113684118A (zh) * 2021-07-14 2021-11-23 浙江大学 一种集成核酸分析芯片
CN114558632A (zh) * 2022-04-26 2022-05-31 广州国家实验室 液体转移装置、多通道液体转移装置以及方法
CN114574349A (zh) * 2022-04-26 2022-06-03 广州国家实验室 一种适配卡盒使用的设备
CN217578917U (zh) * 2022-04-26 2022-10-14 广州国家实验室 样本提取装置
CN114657049A (zh) * 2022-05-12 2022-06-24 广州国家实验室 一种核酸扩增用卡盒

Similar Documents

Publication Publication Date Title
WO2020078410A1 (zh) 样本处理装置及方法，以及包括该处理装置的数字pcr系统
US11446659B2 (en) Nucleic acid extraction apparatus
AU2010256429B2 (en) Universal sample preparation system and use in an integrated analysis system
US8506813B2 (en) Bidirectional transfer of an aliquot of fluid between compartments
US9695467B2 (en) Method for processing nucleic acids-containing fluids
US20140162347A1 (en) Bidirectional transfer of an aliquote of fluid between compartments
WO2024103297A1 (zh) 样品提取卡盒和样品提取方法及核酸检测设备
CN217103902U (zh) 全自动核酸分析检测装置
CN218339833U (zh) 液体转移、多通道液体转移、液体自动提取转移及多通道液体自动提取转移的装置
CN217578917U (zh) 样本提取装置
CN218620840U (zh) 扩增卡盒及核酸检测设备
CN113578411B (zh) 一种移液装置
CN115637209B (zh) 样品提取卡盒和样品提取方法及核酸检测设备
WO2023206094A1 (zh) 一种适配卡盒使用的设备
CN114574349A (zh) 一种适配卡盒使用的设备
CN209024559U (zh) 一种用于全自动化dna提取的48孔深孔板
CN111876313A (zh) 核酸提取装置
CN219194987U (zh) 一种核酸提取装置
CN217628391U (zh) 一种适配卡盒使用的设备
WO2023206093A1 (zh) 样本提取装置
WO2023206092A1 (zh) 液体转移装置、多通道液体转移装置以及方法
CN118086044A (zh) 一种核酸提取装置
CN212222971U (zh) 核酸提取装置
WO2023097922A1 (zh) 一种全封闭全自动核酸提取试剂盒
WO2021248291A1 (zh) 单细胞处理仪器