WO2024088310A1 - Nouveau type de tube de cryoconservation - Google Patents

Nouveau type de tube de cryoconservation Download PDF

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Publication number
WO2024088310A1
WO2024088310A1 PCT/CN2023/126532 CN2023126532W WO2024088310A1 WO 2024088310 A1 WO2024088310 A1 WO 2024088310A1 CN 2023126532 W CN2023126532 W CN 2023126532W WO 2024088310 A1 WO2024088310 A1 WO 2024088310A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
tube body
layer
cap
cryopreservation
Prior art date
Application number
PCT/CN2023/126532
Other languages
English (en)
Chinese (zh)
Inventor
安同艳
王晓菲
王欣
崔丽丽
段文莉
Original Assignee
泰伦特生物工程股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 泰伦特生物工程股份有限公司 filed Critical 泰伦特生物工程股份有限公司
Publication of WO2024088310A1 publication Critical patent/WO2024088310A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
    • Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics

Definitions

  • the utility model relates to the technical field of living body preservation, in particular to a novel cryopreservation tube.
  • Cryotubes are one of the commonly used experimental consumables in biological research and medicine, and are often used for low-temperature transportation and storage of tissue or cell samples.
  • tissue or cell sample libraries In recent years, with the advancement of science and technology and the continuous deepening of medical research, the demand for tissue or cell sample libraries in my country has increased greatly.
  • Many biological companies and medical institutions have their own tissue or cell sample libraries, such as stem cell libraries, tumor tissue libraries, umbilical cord blood libraries, etc.
  • the current sample library has made great progress in standardized management and quality control, it also faces many difficult problems, one of which is the quality and safety of cryotubes.
  • cryopreservation tubes In order to protect the samples in the cryopreservation tubes from contamination or leakage, the current design of cryopreservation tubes mainly considers the sealing problem.
  • the cryopreservation tubes currently sold on the market usually use high-hardness resin materials, external or internal screw-on tube caps, and internal sealing gaskets, but the actual sealing performance is not good, mainly because the cover and the tube body are both hard plastics, and the internal stress of thermal expansion and contraction caused by the temperature difference of the cryopreserved liquid cannot be effectively eliminated, which can easily cause liquid nitrogen to penetrate from the gap between the cover and the tube body, which will not only cause the sample to be contaminated, but also create the risk of tube explosion, posing a safety hazard to both the sample and the experimenter.
  • the purpose of the utility model is to provide a new type of cryopreservation tube, which can solve the problem of poor sealing performance of existing cryopreservation tubes under low temperature conditions.
  • the utility model solves the above technical problems through the following technical means: it includes a tube body and a tube cap, the tube body and the tube cap are connected by rotational engagement, the tube cap includes a tube wall, the tube wall includes a tube body connecting layer, a shrink layer and a protective layer arranged in sequence from the inside to the outside, the shrink layer is connected to the circumference of the tube body connecting layer, the tube body connecting layer is provided with a mutually matching threaded structure on the connecting surface corresponding to the tube body, and a sealing ring is provided on the inner wall of the tube body connecting layer near the tube mouth of the tube cap.
  • the present application provides a shrink layer, which is used for shrinking when the cryopreservation tube in the device is stored at a low temperature, thereby tightening the tube body connecting layer connected to the tube body to be tightly connected to the tube body, thereby increasing the sealing of the tube body and the tube cap when the cryopreservation tube is in a low temperature state, especially when stored in liquid nitrogen.
  • a plurality of adsorption rings are arranged on the inner wall of the tube body connecting layer along the axial direction, and the axis of the adsorption ring is aligned with the inner wall of the tube body connecting layer.
  • the axis lines of the tube body connection layer are collinear, and the tube body is provided with a plurality of adsorption grooves matching the adsorption ring at locations corresponding to the adsorption ring, and the width of the adsorption groove is smaller than the thickness of the adsorption ring.
  • the air in the adsorption groove contracts when it is cooled, and the adsorption ring at the notch of the adsorption groove is sucked into the groove, thereby tightly connecting the tube body connection layer connected to the adsorption ring to the surrounding side of the tube body, and further cooperating with the cold shrink body to seal the connection between the tube body and the tube cap.
  • an explosion-proof cavity is provided in the protective layer, and an explosion-proof layer is provided in the explosion-proof cavity.
  • the present application reduces the risk of explosion of the cryopreservation tube, and more preferably, an explosion-proof layer can be provided in the tube cover and the tube body to increase the safety of the entire cryopreservation tube.
  • the explosion-proof layer is a nylon layer or a glass fiber layer.
  • the tube body is provided with a support frame away from the tube cap end.
  • the present application provides a fan-shaped cryopreservation tube on the tube body to prevent the tube body from being tightly connected to the insertion port and to facilitate the user to unscrew the tube body from the receiving tray.
  • the tube body is provided with scale lines along the height direction.
  • the application provides scale lines corresponding to the sample volume to facilitate the user to observe the sampling volume and record relevant data.
  • the tube cap also includes a tube cover, and a slide groove, a piston and a connecting rod are arranged inside the side of the tube cover connected to the tube wall, the piston is interference-connected with the slide groove, one end of the connecting rod is connected to the piston, and the other end passes through the tube cover and is connected to a turntable arranged outside the tube cover.
  • the piston structure is arranged in the present application, so that when the piston moves outward, the volume of the air in the tube body is expanded, so that the tube body is in a negative pressure environment, thereby preliminarily completing the sealing.
  • the connecting rod is threadedly connected to the pipe cover.
  • the threaded structure is provided to prevent the piston from moving under the action of negative pressure, thereby ensuring the stability of the negative pressure environment.
  • the present application provides a cold shrinkage layer, which is used to shrink when the cryopreservation tube in the device is stored at low temperature, thereby tightening the tube body connection layer connected to the tube body to be tightly connected to the tube body, thereby increasing the sealing of the tube body and the tube cap when the cryopreservation tube is in a low temperature state, especially when stored in liquid nitrogen.
  • the air in the adsorption groove contracts when it is cooled, and the adsorption ring at the groove of the adsorption groove is sucked into the groove, thereby tightly connecting the tube body connecting layer connected to the adsorption ring to the surrounding side of the tube body, and further cooperating with the cold shrink body to seal the connection between the tube body and the tube cap.
  • the present application provides a piston structure so that when the piston moves outward, the volume of the air in the tube body is expanded, creating a negative pressure environment in the tube body, thereby preliminarily completing the sealing.
  • FIG1 is a schematic diagram of the overall structure of the novel cryopreservation tube of the present application.
  • FIG2 is a schematic diagram of the connection between the tube body and the tube cap of the present invention.
  • FIG3 is a cross-sectional view of a pipe cap of the present invention.
  • the new type of cryopreservation tube includes a tube body 1 and a tube cap 2.
  • the tube body 1 is used to hold the sample, and the tube cap 2 is used to seal the tube body 1.
  • the tube body 1 and the tube cap 2 are connected by rotation and snap-fit.
  • the connecting surfaces of the tube body 1 and the tube cap 2 are provided with mutually matching threaded structures, so that the tube body 1 and the tube cap 2 can be rotated and tightened.
  • the tube cap 2 includes a tube wall 3 and a tube cover 4.
  • the tube wall 3 includes a tube body connecting layer 21, a shrink layer 22 and a protective layer 23.
  • the tube body connecting layer 21, the shrink layer 22 and the protective layer 23 are arranged in sequence from the inside to the outside.
  • the tube body connection layer 21 is used for meshing connection with the tube body 1, and a threaded structure is provided on the connection surface of the tube body connection layer 21 corresponding to the tube body 1.
  • the tube body connection layer 21 is connected to the cold shrink layer 22 on the periphery.
  • the cold shrink layer 22 is used to shrink when the cryopreservation tube in the device is stored at low temperature, thereby tightening the tube body connection layer 21 connected to the tube body 1 to be tightly connected to the tube body 1, thereby increasing the sealing of the tube body 1 and the tube cap 2 when the cryopreservation tube is in a low temperature state, especially when stored in liquid nitrogen.
  • a sealing ring 24 is provided on the inner wall of the pipe body connection layer 21 near the pipe opening of the pipe cap 2.
  • the diameter of the sealing ring 24 matches the inner diameter of the pipe cap 2.
  • the sealing ring 24 is used to tightly fit the pipe body 1 when the pipe body connection layer 21 is tightly attached to the pipe body 1 under the contraction pressure of the cold shrink layer 22.
  • the body 1 is interference-connected, and when the temperature is lower, the sealing ring 24 is more tightly connected to the pipe body 1 under the pressure of the cold shrink layer 22, thereby increasing the sealing performance of the pipe cap 2 near the opening.
  • a plurality of adsorption rings 25 are arranged on the inner wall of the tube body connection layer 21 along the axial direction.
  • the axis of the adsorption ring 25 is colinear with the axis of the tube body connection layer 21.
  • a matching number of adsorption grooves 11 are arranged on the circumference of the tube body 1 corresponding to the position of the adsorption ring 25.
  • the adsorption groove 11 and the adsorption ring 25 are arranged so that the adsorption ring 25 can be engaged with the adsorption groove 11 when the tube body 1 and the tube cap 2 are rotated and tightened.
  • the width of the adsorption groove 11 is less than the thickness of the adsorption ring 25.
  • the adsorption ring 25 can completely seal the adsorption groove 11.
  • the air in the adsorption groove 11 contracts when it is cooled, and the adsorption ring 25 in the notch of the adsorption groove 11 will be sucked into the groove, thereby tightly connecting the tube body connection layer 21 connected to the adsorption ring 25 to the circumference of the tube body 1.
  • the adsorption ring 25 can be a soft material with a certain deformation ability such as a silicone ring or a rubber ring.
  • the protective layer 23 is the outermost layer of the tube cap 2, and an explosion-proof cavity 26 is provided in the protective layer 23.
  • An explosion-proof layer 5 is provided in the explosion-proof cavity 26.
  • the explosion-proof layer 5 can be a nylon layer or a glass fiber layer.
  • the present application provides an explosion-proof layer 5 with an explosion-proof function in the protective layer 23 to reduce the risk of bursting of the cryotube.
  • the explosion-proof layer 5 can also be provided in the tube cover 4 and the tube body 1 to increase the safety of the entire cryotube.
  • a support frame 12 is provided at one end of the tube body 1 away from the tube cap 2.
  • the support frame 12 is a fan-shaped cryotube.
  • a cryotube When a cryotube is used, a plurality of cryotubes are generally inserted into a corresponding receiving tray with an insertion port to store a plurality of cryotubes at the same time.
  • the present application provides a fan-shaped cryotube on the tube body 1 to prevent the tube body 1 from being tightly connected to the insertion port and facilitate the user to unscrew the tube body 1 from the receiving tray.
  • the tube body 1 is provided with scale lines along the height direction to display the sample volume in the tube body 1, so that the user can observe the sampling volume and record relevant data.
  • a slide groove 41, a piston 42 and a connecting rod 43 are provided on one side of the tube cover 4 relative to the tube wall 3.
  • the piston 42 is connected to the slide groove 41 by interference fit.
  • One end of the connecting rod 43 is connected to the piston 42, and the other end passes through the slide groove 41 to the outside of the tube cover 4 and is connected to a turntable 44 arranged outside the tube cover 4.
  • the turntable 44 can pull the piston 42 through the connecting rod 43, so that the piston 42 slides in the slide groove 41, wherein when the piston 42 moves outward, the volume of the air in the tube body 1 is expanded, so that the inside of the tube body 1 is a negative pressure environment, thereby preliminarily completing the sealing.
  • the connection between the connecting rod 43 and the tube cover 4 is set to a threaded structure that cooperates with each other.
  • the method of using the utility model is as follows: before the tube body 1 and the tube cap 2 of the cryopreservation tube are tightened and placed in a low-temperature environment for storage, the rotating disk 44 moves the piston to form a negative pressure environment in the tube body 1 to complete the initial sealing. As the temperature decreases, the volume of the cold shrink layer 22 decreases to compress the tube body connection layer 21 connected to the cold shrink layer 22. The tube body connection layer 21 is tightly attached to the tube body 1 under the pressure of the cold shrink layer 22. The shrinkage of the cold shrink layer 22 makes it impossible to effectively eliminate the stress of the tube cap 2 and the tube body 1 at low temperatures. The adsorption groove 11 can also keep the adsorption ring 25 tightly contracted under low temperature, and the sealing performance is greatly improved.
  • the adsorption groove 11 can also absorb part of the adsorption ring 25 into the groove under low temperature, and cooperate with the cold shrink layer 22 to seal the gap at the connection between the tube body 1 and the tube cap 2.
  • the present application also provides an explosion-proof layer to reduce the risk of the cryopreservation tube bursting and improve the safety of the cryopreservation tube when in use.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un nouveau type de tube de cryoconservation comprenant un corps de tube et un capuchon de tube. Le corps de tube et le capuchon de tube sont reliés par encliquetage ; le capuchon de tube comprend une paroi de tube ; la paroi de tube comprend une couche de liaison de corps de tube, une couche rétractable à froid et une couche de protection qui sont agencées séquentiellement de l'intérieur vers l'extérieur ; la couche rétractable à froid est reliée à un côté périphérique de la couche de liaison de corps de tube ; des structures filetées correspondant les unes aux autres sont disposées de manière correspondante sur la surface de liaison de la couche de liaison de corps de tube et du corps de tube ; et une bague d'étanchéité est disposée sur une paroi interne de la couche de liaison de corps de tube à proximité d'un orifice de tube du capuchon de tube. La couche rétractable à froid est conçue pour se rétracter lorsque le tube de cryoconservation dans le dispositif est placé à basse température pour le stockage, de telle sorte que la couche de liaison de corps de tube reliée au corps de tube soit serrée pour être étroitement reliée au corps de tube, ce qui permet d'améliorer les performances d'étanchéité du corps de tube et du capuchon de tube lorsque le tube de cryoconservation est dans un état à basse température, en particulier lorsque le tube de cryoconservation est stocké dans de l'azote liquide.
PCT/CN2023/126532 2022-10-25 2023-10-25 Nouveau type de tube de cryoconservation WO2024088310A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202222811469.6 2022-10-25
CN202222811469.6U CN218942119U (zh) 2022-10-25 2022-10-25 一种新型冻存管

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Publication Number Publication Date
WO2024088310A1 true WO2024088310A1 (fr) 2024-05-02

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PCT/CN2023/126532 WO2024088310A1 (fr) 2022-10-25 2023-10-25 Nouveau type de tube de cryoconservation

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CN (1) CN218942119U (fr)
WO (1) WO2024088310A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN218942119U (zh) * 2022-10-25 2023-05-02 泰伦特生物工程股份有限公司 一种新型冻存管

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN208532786U (zh) * 2018-04-28 2019-02-22 上海原能细胞生物低温设备有限公司 一种冻存管
CN209628482U (zh) * 2019-01-28 2019-11-15 盐城金沃医疗科技有限公司 一种多层密封冻存管
CN209643682U (zh) * 2019-02-25 2019-11-19 上海尚泰生物技术有限公司 冻存管
CN213428004U (zh) * 2020-09-09 2021-06-15 上海琛耳生物科技中心 一种新型冷存管
CN213695471U (zh) * 2020-10-30 2021-07-16 标普(常州)生物科技有限公司 一种便于储存的冻存管
CN214385784U (zh) * 2021-03-01 2021-10-15 广州准优生物科技有限公司 细胞冻存管
CN214991537U (zh) * 2021-07-01 2021-12-03 宁波鸿鼎医疗器械科技有限公司 一种冻存管
CN216164778U (zh) * 2020-12-10 2022-04-05 苏州贝康智能制造有限公司 冻存管
CN218942119U (zh) * 2022-10-25 2023-05-02 泰伦特生物工程股份有限公司 一种新型冻存管

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN208532786U (zh) * 2018-04-28 2019-02-22 上海原能细胞生物低温设备有限公司 一种冻存管
CN209628482U (zh) * 2019-01-28 2019-11-15 盐城金沃医疗科技有限公司 一种多层密封冻存管
CN209643682U (zh) * 2019-02-25 2019-11-19 上海尚泰生物技术有限公司 冻存管
CN213428004U (zh) * 2020-09-09 2021-06-15 上海琛耳生物科技中心 一种新型冷存管
CN213695471U (zh) * 2020-10-30 2021-07-16 标普(常州)生物科技有限公司 一种便于储存的冻存管
CN216164778U (zh) * 2020-12-10 2022-04-05 苏州贝康智能制造有限公司 冻存管
CN214385784U (zh) * 2021-03-01 2021-10-15 广州准优生物科技有限公司 细胞冻存管
CN214991537U (zh) * 2021-07-01 2021-12-03 宁波鸿鼎医疗器械科技有限公司 一种冻存管
CN218942119U (zh) * 2022-10-25 2023-05-02 泰伦特生物工程股份有限公司 一种新型冻存管

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