WO2024086213A2 - Système de lyse cellulaire et d'isolation de plasmide - Google Patents

Système de lyse cellulaire et d'isolation de plasmide Download PDF

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Publication number
WO2024086213A2
WO2024086213A2 PCT/US2023/035392 US2023035392W WO2024086213A2 WO 2024086213 A2 WO2024086213 A2 WO 2024086213A2 US 2023035392 W US2023035392 W US 2023035392W WO 2024086213 A2 WO2024086213 A2 WO 2024086213A2
Authority
WO
WIPO (PCT)
Prior art keywords
container
fluidic
fluid
tubing
fluidly connected
Prior art date
Application number
PCT/US2023/035392
Other languages
English (en)
Inventor
Brady COLE
Christopher Boyce
Jeffrey FARLEY
John KIEHART
Sebastian Almeida
Original Assignee
Abec, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abec, Inc. filed Critical Abec, Inc.
Publication of WO2024086213A2 publication Critical patent/WO2024086213A2/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Abstract

La présente divulgation concerne un équipement, des systèmes et des procédés pour isoler des acides nucléiques de cellules, de préférence de manière continue, à l'aide d'un système de réacteur.
PCT/US2023/035392 2022-10-19 2023-10-18 Système de lyse cellulaire et d'isolation de plasmide WO2024086213A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202263417630P 2022-10-19 2022-10-19
US63/417,630 2022-10-19
US202363468322P 2023-05-23 2023-05-23
US63/468,322 2023-05-23

Publications (1)

Publication Number Publication Date
WO2024086213A2 true WO2024086213A2 (fr) 2024-04-25

Family

ID=88793177

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/035392 WO2024086213A2 (fr) 2022-10-19 2023-10-18 Système de lyse cellulaire et d'isolation de plasmide

Country Status (1)

Country Link
WO (1) WO2024086213A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8658419B2 (en) 2009-09-04 2014-02-25 Abec, Inc. Heat transfer baffle system and uses thereof
US9228165B2 (en) 2011-03-15 2016-01-05 Abec, Inc. Reactor systems
WO2019070648A2 (fr) 2017-10-03 2019-04-11 Abec, Inc. Systèmes de réacteur

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8658419B2 (en) 2009-09-04 2014-02-25 Abec, Inc. Heat transfer baffle system and uses thereof
US9228165B2 (en) 2011-03-15 2016-01-05 Abec, Inc. Reactor systems
WO2019070648A2 (fr) 2017-10-03 2019-04-11 Abec, Inc. Systèmes de réacteur

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