WO2024080722A1

WO2024080722A1 - Microfluidic flow velocity precise-control device using synchronously operating dual valves and microfluidic flow velocity precise-control method using same

Info

Publication number: WO2024080722A1
Application number: PCT/KR2023/015589
Authority: WO
Inventors: 박상열; 양인철; 유희봉
Original assignee: 한국표준과학연구원
Priority date: 2022-10-11
Filing date: 2023-10-11
Publication date: 2024-04-18
Also published as: KR102524384B1

Abstract

The present invention relates to a microfluidic flow velocity precise-control device using synchronously operating dual valves and a microfluidic flow velocity precise-control method using same, wherein the microfluidic flow velocity precise-control device using synchronously operating dual valves and the microfluidic flow velocity precise-control method using same are configured to perform synchronized operations in order to precisely control the flow velocity of a microfluid. Therefore, the present invention can precisely control the flow velocity of a microfluid.

Description

Microfluidic flow rate precision control device using synchronized operation double valve and microfluidic flow rate precision control method using the same

The present invention relates to a microfluidic flow rate precision control device using a synchronized double valve and a method for precisely controlling the microfluidic flow rate using the same. More specifically, the present invention relates to a microfluidic flow rate using a synchronized double valve to synchronize the microfluidic flow rate. It relates to a precision control device and a method of precisely controlling the microfluidic flow rate using the same.

Microfluidic devices are used in many fields, including various biochemical analyzes and biological sample analysis for disease diagnosis. Microfluidic devices include micro-devices such as capillary channels, microfluidic chips, and lab-on-a-chips, and include microfluidic channels and microfluidic channels. Provide a structure.

Various types of microfluidic devices as described above can be used to analyze specific components in chemical or biochemical samples, and can be used for culturing tissue cells in a micro-chamber and drug testing using them. Additionally, a chemical reaction can occur by mixing two or more sample solutions within a microfluidic device, and it can also be used to manufacture functional materials. In this way, microfluidic devices can be applied to various fields and are being used for various purposes in various fields.

The amount of sample solution injected into the microfluidic tube of the microfluidic device is very small, and therefore it is important to control the movement of the extremely small amount of sample solution to be injected into the microfluidic tube.

However, the amount of sample solution injected into the microfluidic tube of the microfluidic device is extremely small, and therefore it is important to control the movement of the extremely small amount of sample solution to be injected into the microfluidic tube, but there are realistically difficult problems as follows. In the case of fluid flowing through a large diameter conduit, the transport of the fluid is easily controlled by adding pressure or vacuum. However, in the case of fluid within a microfluidic tube, the friction force acting on the wall of the fluid tube is relatively large, and when a large pressure or vacuum is applied to overcome this, the flow speed becomes very fast, making it difficult to transport a small amount of fluid.

In the conventional Korean Patent No. 10-2341588 (December 16, 2021), a fluid bridge is formed between the first and second plates, and then the fluid is sucked through a flow channel, thereby forming a fluid bridge between the first and second plates. Since the contact angle of each fluid contact portion becomes small, a microfluidic control device and a microfluidic control method using the same that can precisely control the amount of fluid by separating the fluid legs have been disclosed.

However, conventional microfluidic control devices have the problem that it is difficult to finely control the desired amount of fluid.

Therefore, the present invention was created to solve the problems of the prior art as described above, and the purpose of the present invention is to precisely control the flow rate of the microfluidic fluid using a synchronized double valve. It relates to a precision control device and a method of precisely controlling the microfluidic flow rate using the same.

The microfluidic control device 1000 using a synchronized double valve according to the present invention includes a microfluidic tube 100 in the form of a capillary tube; A pressure transmission passage 200 connected to one side of the microfluidic pipe 100; A first synchronization unit connected to one side of the pressure transmission passage 200; a second synchronization unit connected to the other side of the pressure transmission passage 200; and a synchronization valve controller 600 connected in series with the first control unit and the second control unit; may include.

Next, the microfluidic control method using a double valve according to the present invention includes a synchronization valve controller 600 connected to one side of the pressure transmission passage 200 connected to one side of the microfluidic pipe 100 and connected to the first conductor 610. ) A first synchronization step (S100) of controlling the first synchronization valve 310 by ; Second synchronization that controls the second synchronization valve 320 by the synchronization valve controller 600 connected to the other side of the pressure transfer passage 200 connected to one side of the microfluidic pipe 100 and connected to the second conductor 620. Step (S200); may include.

Therefore, the microfluidic flow rate precision control device using the synchronized double valve of the present invention is capable of synchronized operation in which the first synchronization valve and the first synchronization valve are operated at a certain time difference (δt), thereby allowing the microfluidic flow in the microfluidic pipe. This can be precisely controlled.

In addition, the microfluidic flow rate precision control device using the synchronized operation double valve of the present invention can arbitrarily adjust the strength and operating time of the effective vacuum delivered to the microfluidic tube connected to the pressure transmission channel, so that the Microfluidic flow can be precisely controlled and various flow rates of microfluidic can be obtained.

Figure 1 is a schematic diagram of a microfluidic control device using a synchronized double valve of the present invention.

Figure 2 is a step diagram of the microfluidic control method using the synchronized operation double valve of the present invention.

Figure 3 is a schematic diagram according to Example 1 of a microfluidic control device using a synchronized operation double valve of the present invention.

Figure 4 is a schematic diagram according to Example 2 of a microfluidic control device using a synchronized double valve of the present invention.

Figure 5 is a graph of synchronization operation according to the time difference between the first synchronization valve and the second synchronization valve according to Examples 1 and 2 of the microfluidic control device using the synchronization operation double valve of the present invention.

Figure 6 shows the experimental results of operating the microfluidic control device using the synchronized double valve of the present invention.

Figure 7 is an actual photo of the microfluidic control device using the synchronized double valve of the present invention.

In addition, the first control unit includes a first synchronization valve 310 connected to one side of the pressure transmission passage 200, and a first pressure source connected to the first synchronization valve 310 and a first conduit to apply pressure. (510), it may include the synchronization valve controller 600 that is connected to the first synchronization valve 310 and the first conductor 610 and controls the applied time.

In addition, the second control unit is connected to the second synchronization valve 320 connected to the other side of the pressure transmission passage 200, and the second synchronization valve 320 and the second conduit 420 to which pressure is applied. It may include a second pressure source 520, the second synchronization valve 320, and the synchronization valve controller 600 that is connected to the second conductor 620 and controls the applied time.

Additionally, the synchronization valve controller may cause the first synchronization valve 310 and the second synchronization valve 320 to operate at preset time intervals.

Additionally, the synchronization valve controller may allow the first synchronization valve 310 and the second synchronization valve 320 to operate with a constant time difference (δt) in the range of 0 to ±100 ms.

Additionally, the microfluidic tube 100 may contain fluid in the range of 0.1 to 500 μL.

Additionally, the first synchronization valve 310 may allow negative or positive pressure to be applied to the pressure transmission passage 200 by operating the first pressure source 510.

Additionally, the second synchronization valve 320 can adjust the pressure applied to the pressure transmission passage 200 by the operation of the second pressure source 520.

In addition, the first synchronization step (S100) is a first pressure application step in which pressure is applied to the first synchronization valve 310 by the first pressure source 510 connected to the first synchronization valve 310 and the first conduit. (S110), after the first pressure application step (S110), the time applied to the first synchronization valve 310 by the synchronization valve controller 600 connected to the first synchronization valve 310 and the first conductor 610 is It may include a controlled first control step (S120).

In addition, the second synchronization step (S200) is a second synchronization valve in which pressure is applied to the second synchronization valve 320 by the second pressure source 520 connected to the second synchronization valve 320 and the second conduit 420. Pressure application step (S210), after the second pressure application step (S210), the pressure is applied to the second synchronization valve 320 by the synchronization valve controller 600 connected to the second synchronization valve 320 and the second conductor 620. It may include a second control step (S220) in which the time is controlled.

In addition, in the first synchronization step (S100) and the second synchronization step (S200), the first synchronization valve 310 and the second synchronization valve 320 are adjusted by the synchronization valve controller 600 with a constant time difference ( δt) can be operated.

In addition, in the first synchronization step (S100) and the second synchronization step (S200), the first synchronization valve 310 and the second synchronization valve 320 are operated by the synchronization valve controller 600 from 0 to ± It can be operated with a constant time difference (δt) in the range of 100 ms.

There is a problem in that it is difficult to control the flow rate by applying positive or negative pressure (vacuum) due to the friction between the fluid and the pipe wall, which has a relatively large effect on the microfluid in a microfluidic device. In particular, if a sufficiently large pressure or vacuum is applied to overcome the friction force, the flow rate becomes very fast, making it impossible to precisely control the flow rate at low speeds for handling trace samples. This phenomenon can slow down the development of biochemical microanalysis technology that handles trace samples and reagents. Therefore, the present invention controls the synchronization valves to be synchronized with a synchronization valve controller for microfluidic control, thereby solving the above problems by precisely controlling the flow of microfluids in the microtubes.

Hereinafter, the present invention will be described in more detail through specific examples or examples including the attached drawings. However, the following specific examples or examples are only a reference for explaining the present invention in detail, and the present invention is not limited thereto, and may be implemented in various forms.

Additionally, unless otherwise defined, all technical and scientific terms have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used for description herein is merely to effectively describe particular embodiments and is not intended to limit the invention.

Additionally, as used in the specification and appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms as well, unless the context clearly dictates otherwise.

Additionally, when a part "includes" a certain component, this means that it may further include other components rather than excluding other components unless specifically stated to the contrary.

Figure 1 shows a schematic diagram of a microfluidic control device using a synchronized double valve. Referring to Figure 1, the microfluidic control device 1000 using a synchronized double valve of the present invention includes a microfluidic tube 100 in the form of a capillary tube. A pressure transfer passage 200 connected to one side of the microfluidic pipe 100, a first control unit connected to one side of the pressure transfer passage 200, and a second control unit connected to the other side of the pressure transfer passage 200. It includes a synchronization valve controller 600 connected in series with the first control unit and the second control unit.

Here, the microfluidic control device 1000 using the synchronized double valve can be used to precisely control the flow of microfluid in the microfluidic tube 100, so one side of the microfluidic tube 100 is pressure It may be connected to the delivery passage 200, and the other side may be immersed in the sample solution 10 in the sample solution 10 tank. Therefore, a small amount of the sample solution 10 can be sucked in or discharged precisely by operating the microfluidic control device 1000 using the synchronized double valve using the microfluidic tube 100. Here, the diameter of the microfluidic tube may be 0.01 to 1 mm. If the diameter of the microfluidic tube is smaller than 0.01 mm, the applied pressure must be increased, but if the pressure increases, precise microfluidic control is impossible. Additionally, if the diameter of the microfluidic tube is larger than 1 mm, the flow rate of the microfluid may increase compared to the applied pressure, making it difficult to control the flow rate of the microfluid. Therefore, in the present invention, the diameter of the microfluidic pipe was designed to be in the range of 0.01 to 1 mm to enable smooth control of the flow rate of microfluidic.

In addition, the first control unit is connected to one side of the pressure transmission passage 200 to control the flow of fluid, and is connected to the first synchronization valve 310 and the first conduit to control the flow of fluid. It includes a first pressure source 510 to be applied, and a synchronization valve controller 600 that is connected to the first synchronization valve 310 and a first conductor 610 to control the applied pressure.

also. Here, the second control unit is connected to the other side of the pressure transfer passage 200 and includes a second synchronization valve 320 that controls the flow of fluid, the second synchronization valve 320, and the second conduit 420. It includes a second pressure source 520 that is connected to apply pressure, and the synchronization valve controller 600 that is connected to the second synchronization valve 320 and a second conductor 620 to control the applied pressure.

The synchronization valve controller can control the operation of the first synchronization valve 310 and the second synchronization valve 320. Here, the first pressure source 510 and the second pressure source 520 may have different pressure strengths. The first pressure source 510 and the second input source may be connected to the first synchronization valve 310 and the second synchronization valve 320 through the first conduit and the second conduit 420. Pressure may be applied by the pressure source 510 and the second pressure source 520. Additionally, the first synchronization valve 310 and the second synchronization valve 320 may be operated with a constant time difference (δt) by control of the synchronization valve controller. Accordingly, the effective pressure intensity applied within the pressure transmission passage 200 is precisely adjusted, so that the flow of a small amount of fluid contained in the microfluidic tube 100 can be precisely controlled. Here, the microfluidic tube 100 may contain fluid in the range of 0.1 to 500 μL. More specifically, the first pressure source 510 and the second pressure source 520 have a mutual pressure difference and positive or negative pressure is applied to the pressure transmission passage 200 by the synchronization valve controller. It can be added, and as a result, it is possible to control the flow rate of microfluid by adjusting the suction force in the microfluidic pipe 100 connected to the pressure transmission passage 200.

Figure 2 shows a step diagram of the microfluidic flow rate precision control method using the synchronized operation double valve of the present invention. Referring to the microfluidic flow rate precision control method (S1000) using the synchronized operation double valve of the present invention in Figure 3, it is connected to one side of the pressure transmission passage 200 connected to one side of the microfluidic pipe 100, and the first conductor ( The first synchronization step (S100) controls the first synchronization valve 310 by the synchronization valve controller 600 connected to 610), and is connected to the other side of the pressure transfer passage 200 connected to one side of the microfluidic pipe 100. , It may include a second synchronization step (S200) in which the second synchronization valve 320 is controlled by the synchronization valve controller 600 connected to the second conductor 620.

Here, the first synchronization step (S100) is a first pressure applied to the first synchronization valve 310 by the first pressure source 510 connected to the first synchronization valve 310 and the first conduit. Step (S110), the time applied to the first synchronization valve 310 by the synchronization valve controller 600 connected to the first synchronization valve 310 and the first conductor 610 after the first pressure application step (S110). This control may further include a first control step (S120).

In addition, here, in the second synchronization step (S200), pressure is applied to the second synchronization valve 320 by the second pressure source 520 connected to the second synchronization valve 320 and the second conduit 420. In the second pressure application step (S210), after the second pressure application step (S210), the second synchronization valve 320 is operated by the synchronization valve controller 600 connected to the second synchronization valve 320 and the second conductor 620. ) may further include a second control step (S220) in which the time applied to is controlled.

Next, the method for precisely controlling the microfluidic flow rate using the synchronized double valve of the present invention will be described as follows. In the first synchronization step (S100), pressure may be applied to the first synchronization valve 310 by the first pressure source 510, and the pressure applied by the first pressure source 510 may be transferred to the synchronization valve controller ( The time applied can be controlled by 600). In addition, in the second synchronization step (S200), pressure may be applied to the second synchronization valve 310 by the second pressure source 520, and the pressure applied by the second pressure source 520 may be applied to the synchronization controller. The time applied can be controlled by .

Therefore, the first synchronization valve 310 and the second synchronization valve 320 can be operated with a constant time difference (δt) by the synchronization valve controller 600, and more specifically, the synchronization valve controller 600 ), the first synchronization valve 310 and the second synchronization valve 320 can be operated with a constant time difference (δt) in the range of 0 to ±100 ms, so that the degree of vacuum is adjusted by a small difference, so that the microfluidic tube (100) ) The microfluidic flow can be precisely controlled and various flow rates of microfluidic can be obtained.

Figure 3 shows a schematic diagram according to Example 1 of a microfluidic control device using a synchronized operation double valve of the present invention, and Figure 4 shows a schematic diagram according to Example 2 of a microfluidic control device using a synchronized operation double valve of the present invention. It represents.

With reference to FIGS. 3 and 4, Example 1 and Example 2 of the method for precisely controlling the microfluidic flow rate using a microfluidic flow rate precise control device using a synchronized double valve will be described in detail.

The first pressure source 510 used was an NF30-KFDC diaphragm pressure/vacuum pump from KNF, and in the embodiment of the present invention, a self-made plastic buffer container with a capacity of 100 mL was connected and used. The vacuum degree of the second pressure source 520 (pressure/vacuum pump) was maintained at -55 to -65 kPa. At this time, NXP Semiconductor's MPX5100DP, a pressure/vacuum sensor, was used to measure the vacuum level.

The first synchronization valve 310 and the second synchronization valve 320 used CKD's UMB1-T1 solenoid valve, respectively. The pressure transfer passage used a T-shaped connector to be connected to the first synchronization valve and the second synchronization valve 320.

Here, as shown in FIG. 3, as in Example 1, one side of the first synchronization valve 310 was connected to the first pressure source 510, and the other side was connected to the pressure transmission passage 200. . In addition, one side of the second synchronization valve 320 is connected to the pressure transmission passage 200, and the other side is a silicone tube with an outer diameter of 3 mm and an inner diameter of 1.0 mm to form an atmospheric pressure inlet pipe 530. and was connected using a transparent FEP tube with an outer diameter of 1.6 mm and an inner diameter of 0.5 mm.

In addition, here, as shown in FIG. 4, in Example 2, one side of the first synchronization valve 310 is connected to the pressure transmission passage 200, and the other side is connected to the pressure transmission passage 200. did. In addition, one side of the second synchronization valve 320 is connected to the second pressure source 520, and the other side is made of silicone with an outer diameter of 3 mm and an inner diameter of 1.0 mm to form an atmospheric pressure inlet pipe 530. The tube was connected using a transparent FEP tube with an outer diameter of 1.6 mm and an inner diameter of 0.5 mm.

In addition, the microfluidic tube 100 used PEEK tubing with an inner diameter of 0.175 mm, an outer diameter of 1.6 mm, and a length of 60 mm.

In addition, a synchronization valve controller 600 was used to operate the first synchronization valve 310 and the second synchronization valve 320 at a constant time difference (δt), and the synchronization valve controller 600 operates at a constant time difference (δt). In order to operate it, it was programmed with Arduino to control the operation of the first synchronization valve 310 and the second synchronization valve 320 through the synchronization valve controller 600 and obtain a signal. In this process, the first synchronization valve 310 and the second synchronization valve 320 were configured to be synchronized by turning on/off the 24V operating power.

In this embodiment, the ON operation time difference between the first synchronization valve 310 and the second synchronization valve 320 was changed between -10 ms and +10 ms. Here, when the ON time of the first synchronization valve 310 is ahead of the ON time of the second synchronization valve 320, the time difference δt is expressed as a positive value. At this time, the first synchronization valve 310 may be connected to a vacuum source and the second synchronization valve 320 may be connected to the atmosphere. Here, after vacuum is applied to the first synchronization valve 310, atmospheric pressure may be applied to the second synchronization valve 320. At this time, the vacuum applied to the first synchronization valve may be released into the atmosphere, and the second synchronization valve may be opened into the atmosphere to apply atmospheric pressure.

Additionally, when the ON time of the first synchronization valve 310 lags behind the ON time of the second synchronization valve 320, the time difference δt is expressed as a negative value. At this time, the first synchronization valve 310 may be connected to the atmosphere and the second synchronization valve 320 may be connected to a vacuum source. Here, after atmospheric pressure is applied to the first synchronization valve 310, vacuum may be applied to the second synchronization valve 320. At this time, the atmospheric pressure applied to the first synchronization valve 310 is released and a vacuum may be applied to the first synchronization valve.

In addition, in order to measure the moving speed of the microfluid within the microfluidic tube 100, the other side of the microfluidic tube 100 was immersed in the sample solution 10, where the sample solution 10 was black ink. solution was used.

In the above example, the results of measuring the moving speed of the sample solution 10 (black ink solution) in the microfluidic tube 100 are divided into Example 1 and Example 2 and are shown in <Table 1> below. 5 shows a synchronization operation graph according to the time difference between the first synchronization valve and the second synchronization valve according to Examples 1 and 2 of the microfluidic control device using the synchronization operation double valve of the present invention.

Referring to FIG. 3, the first synchronization valve 310 is connected to a vacuum source to apply vacuum, and the second synchronization valve is connected to the atmosphere to apply atmospheric pressure. Referring to FIG. 5(a), a synchronization operation is possible in which the second synchronization valve 320 connected to the atmosphere is operated at a certain time difference (δt) after the first synchronization valve 310 connected to the vacuum source is operated. . Referring to Example 1 in <Table 1> below, after the first synchronization valve 310 connected to the vacuum source is operated, the second synchronization valve 320 connected to the atmosphere is operated with a certain time difference (δt). The suction flow rate per second of the microfluidic tube can be increased. To explain in detail, it can be seen that as the time difference changes from 10 to -10 ms, the suction flow rate of the sample solution sucked into the microfluidic tube 100 decreases. Here, the first synchronization valve 310 may be connected to a vacuum source and the second synchronization valve 320 may be connected to the atmosphere. After the vacuum source is applied to the first synchronization valve 310, the second synchronization valve 320 may be connected to the atmosphere. Atmospheric pressure may be applied to the synchronization valve 320. Therefore, by applying a very weak vacuum, a very small amount of fluid can be sucked in, making very fine control possible.

Referring to FIG. 4, the first synchronization valve 310 is connected to the atmosphere to apply atmospheric pressure, and the second valve is connected to a vacuum source to apply vacuum. Referring to FIG. 5(b), a synchronization operation is possible in which the second synchronization valve 320 connected to the vacuum source is operated at a certain time difference (δt) after the first synchronization valve 310 connected to the atmosphere is operated. . Referring to Example 2 in <Table 1> below, after the first synchronization valve 310 connected to the atmosphere is operated, the second synchronization valve 320 connected to the vacuum source is operated with a certain time difference (δt). The suction flow rate per second of the microfluidic tube can be reduced, so that a very small amount of microfluidic flow can be controlled as the suction flow rate per second is reduced. To explain in detail, it can be seen that as the time difference changes from 0 to 10 ms, the suction flow rate of the sample solution sucked into the microfluidic tube increases. Here, the first synchronization valve 310 is connected to the atmosphere, and the second synchronization valve 320 is connected to a vacuum source so that vacuum can be applied. Therefore, after the vacuum source is applied to the first synchronization valve 310, a vacuum of stronger intensity can be applied than when atmospheric pressure is applied to the second synchronization valve 320, thereby allowing a relatively small amount of fluid to be released. It can be suctioned, making it possible to control the flow rate of microfluidic.

As shown in Examples 1 and 2 of Table 1 below, when the time difference (δt) is changed from -10 ms to 10 ms, the suction flow rate of the fluid sucked into the microfluidic tube 100 is 0.15 μL/s. It was confirmed that it can be varied up to 1.54 μL/s. Therefore, as the first synchronization valve and the second synchronization valve are connected to the atmosphere or a vacuum source, the strength and operating time of the effective vacuum delivered to the microfluidic pipe 100 connected to the pressure transfer passage 200 are arbitrary. It can be adjusted accordingly. Accordingly, the microfluidic flow within the microfluidic tube 100 can be precisely controlled.

It was confirmed that the suction flow rate of several μL of microfluid within the microfluidic tube 100 can be precisely controlled by applying vacuum and atmospheric pressure using the microfluidic control device 1000 using the synchronized double valve of the present invention. did.

Figure 6 shows the experimental results of operating the microfluidic control device using the synchronized double valve of the present invention, and Figure 7 is an actual photo of the microfluidic control device using the synchronized double valve of the present invention. This shows an actual photo of a microfluidic control device using a valve. As shown in FIGS. 6 and 7, the first synchronization valve 310 and the second synchronization valve 320 maintain a constant time difference (δt) using the microfluidic flow rate precision control device and method using the synchronization operation double valve of the present invention. ) is possible, so the microfluidic flow in the microfluidic tube 100 can be precisely controlled.

In addition, the strength and operating time of the effective vacuum delivered to the microfluidic tube 100 connected to the pressure transfer passage 200 can be configured to be arbitrarily adjusted, so the microfluidic flow in the microfluidic tube 100 can be precisely controlled. The flow rate of the microfluid can be adjusted in various ways depending on the purpose.

Therefore, by precisely controlling the transport of microfluidic samples within the microchannel, it is possible to easily and simply control the flow rate of microfluids in fields that require various microfluids, such as microanalysis and microreactions. Because no separate special-purpose devices or parts are required for this, precise control of the flow rate of microfluidics can be implemented simply and economically.

The present invention is not limited to the above-described embodiments, and the scope of application is diverse. Of course, various modifications and implementations are possible without departing from the gist of the present invention as claimed in the claims.

The microfluidic flow rate precision control device using a synchronized double valve of the present invention and the microfluidic flow rate precision control method using the same can easily and simply control the flow rate of microfluids in fields that require various microfluids, and Precise control of flow rate can be implemented simply and economically.

Claims

Capillary-shaped microfluidic tube;

A pressure transmission passage connected to one side of the microfluidic tube;

A first synchronization unit connected to one side of the pressure transmission passage;

a second synchronization unit connected to the other side of the pressure transmission passage; and

A synchronization valve controller connected in series with the first control unit and the second control unit;

Microfluidic control device using a synchronized double valve including.
The method of claim 1, wherein the first control unit,

A first synchronization valve connected to one side of the pressure transmission passage,

A first pressure source connected to the first synchronization valve and the first conduit to apply pressure,

A microfluidic control device using a synchronization double valve including the first synchronization valve and the synchronization valve controller that is connected to the first conductor and controls the applied time.
The method of claim 1, wherein the second control unit,

A second synchronization valve connected to the other side of the pressure transfer passage,

A second pressure source connected to the second synchronization valve and a second conduit to apply pressure,

A microfluidic control device using a synchronization double valve including the synchronization valve controller that is connected to the second synchronization valve and a second conductor wire to control the applied time.
The method of claim 1, wherein the synchronization valve controller,

A microfluidic control device using a synchronized double valve that operates the first synchronization valve and the second synchronization valve at preset time intervals.
The method of claim 2, wherein the synchronization valve controller,

A microfluidic control device using a synchronization double valve in which the synchronization valve controller operates the first synchronization valve and the second synchronization valve with a constant time difference (δt) in the range of 0 to ±100 ms.
The method of claim 1, wherein the microfluidic tube is:

Microfluidic control device using synchronized dual valves containing fluids ranging from 0.1 to 500 μL.
The method of claim 1, wherein the first synchronization valve is:

A microfluidic control device using a synchronized double valve that allows negative or positive pressure to be applied to the pressure transmission passage by operation of the first pressure source.
The method of claim 1, wherein the second synchronization valve is:

A microfluidic control device using a double valve that adjusts the pressure applied in the pressure transmission passage by operating the second pressure source.
The microfluidic control method using a double valve according to any one of claims 1 to 8,

A first synchronization step of controlling the first synchronization valve by a synchronization valve controller connected to one side of the pressure transmission passage connected to one side of the microfluidic pipe and connected to the first conductor;

A second synchronization step of controlling the second synchronization valve by a synchronization valve controller connected to the other side of the pressure transmission passage connected to one side of the microfluidic pipe and connected to the second conductor;

Microfluidic control method using a double valve including.
The method of claim 9, wherein the first synchronization step is,

A first pressure application step in which pressure is applied to the first synchronization valve by a first pressure source connected to the first synchronization valve and the first conduit,

A microfluidic control method using a double valve including a first control step in which the time applied to the first synchronization valve is controlled by a synchronization valve controller connected to the first synchronization valve and a first conductor after the first pressure application step.
The method of claim 9, wherein the second synchronization step is:

A second pressure application step in which pressure is applied to the second synchronization valve by a second pressure source connected to the second synchronization valve and the second conduit,

A microfluidic control method using a double valve including a second control step in which the time applied to the second synchronization valve is controlled by a synchronization valve controller connected to the second synchronization valve and a second conductor after the second pressure application step.
The method of claim 9, wherein the first synchronization step and the second synchronization step are:

A microfluidic control method using a double valve in which the first synchronization valve and the second synchronization valve are operated with a constant time difference (δt) by the synchronization valve controller.
The method of claim 12, wherein the first synchronization step and the second synchronization step are:

A microfluidic control method using a synchronization double valve in which the first synchronization valve and the second synchronization valve are operated by the synchronization valve controller with a constant time difference (δt) in the range of 0 to ±100 ms.