WO2024077281A3 - Genotyping and sequencing assays for prrsv - Google Patents

Genotyping and sequencing assays for prrsv Download PDF

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Publication number
WO2024077281A3
WO2024077281A3 PCT/US2023/076300 US2023076300W WO2024077281A3 WO 2024077281 A3 WO2024077281 A3 WO 2024077281A3 US 2023076300 W US2023076300 W US 2023076300W WO 2024077281 A3 WO2024077281 A3 WO 2024077281A3
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WO
WIPO (PCT)
Prior art keywords
prrsv
genotyping
sequencing assays
vitro assays
assays
Prior art date
Application number
PCT/US2023/076300
Other languages
French (fr)
Other versions
WO2024077281A2 (en
Inventor
Leonardo CASERTA
Diego DIEL
Original Assignee
Cornell University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell University filed Critical Cornell University
Publication of WO2024077281A2 publication Critical patent/WO2024077281A2/en
Publication of WO2024077281A3 publication Critical patent/WO2024077281A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present disclosure provides in vitro assays for genotyping a porcine reproductive and respiratory syndrome virus (PRRSV). Methods and kits for the in vitro assays are also provided.
PCT/US2023/076300 2022-10-06 2023-10-06 Genotyping and sequencing assays for prrsv WO2024077281A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263413744P 2022-10-06 2022-10-06
US63/413,744 2022-10-06

Publications (2)

Publication Number Publication Date
WO2024077281A2 WO2024077281A2 (en) 2024-04-11
WO2024077281A3 true WO2024077281A3 (en) 2024-05-30

Family

ID=90609081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/076300 WO2024077281A2 (en) 2022-10-06 2023-10-06 Genotyping and sequencing assays for prrsv

Country Status (1)

Country Link
WO (1) WO2024077281A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140271698A1 (en) * 2013-03-15 2014-09-18 Boehringer Ingelheim Vetmedica, Inc. Porcine reproductive and respiratory syndrome virus, compositions, vaccine and methods of use
US20160317642A1 (en) * 2013-12-20 2016-11-03 Boehringer Ingelheim Vetmedica Gmbh Prrs virus variant, european prrs virus cdna clone, and uses thereof
US20190352709A1 (en) * 2016-12-01 2019-11-21 Oxford Nanopore Technologies Limited Methods and systems for characterizing analytes using nanopores
US20220241393A1 (en) * 2019-08-29 2022-08-04 ELANCO US, Inc. Porcine reproductive and respiratory syndrome vaccine virus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140271698A1 (en) * 2013-03-15 2014-09-18 Boehringer Ingelheim Vetmedica, Inc. Porcine reproductive and respiratory syndrome virus, compositions, vaccine and methods of use
US20160317642A1 (en) * 2013-12-20 2016-11-03 Boehringer Ingelheim Vetmedica Gmbh Prrs virus variant, european prrs virus cdna clone, and uses thereof
US20190352709A1 (en) * 2016-12-01 2019-11-21 Oxford Nanopore Technologies Limited Methods and systems for characterizing analytes using nanopores
US20220241393A1 (en) * 2019-08-29 2022-08-04 ELANCO US, Inc. Porcine reproductive and respiratory syndrome vaccine virus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TIAN. ET AL.: "Rapid visual detection of porcine reproductive and respiratory syndrome virus via recombinase polymerase amplification combined with a lateral flow dipstick", ARCHIVES OF VIROLOGY, vol. 167, no. 2, 7 January 2022 (2022-01-07), pages 493 - 499, XP037691900, DOI: 10.1007/s00705-021-05349-8 *

Also Published As

Publication number Publication date
WO2024077281A2 (en) 2024-04-11

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