WO2024062641A1 - Image sensor cover member, observation system, and observation method - Google Patents

Image sensor cover member, observation system, and observation method Download PDF

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Publication number
WO2024062641A1
WO2024062641A1 PCT/JP2023/003218 JP2023003218W WO2024062641A1 WO 2024062641 A1 WO2024062641 A1 WO 2024062641A1 JP 2023003218 W JP2023003218 W JP 2023003218W WO 2024062641 A1 WO2024062641 A1 WO 2024062641A1
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Prior art keywords
sample
cover member
image sensor
imaging device
image
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PCT/JP2023/003218
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French (fr)
Japanese (ja)
Inventor
理映子 水内
敏弘 今田
勇太 橋本
武士 松代
由紀夫 平岡
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株式会社 東芝
東芝インフラシステムズ株式会社
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Publication of WO2024062641A1 publication Critical patent/WO2024062641A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B5/00—Optical elements other than lenses

Definitions

  • Embodiments of the present invention relate to a cover member for an image sensor, an observation system, and an observation method.
  • an imaging device called an on-chip image sensor (a device that can capture images by placing a sample directly on the sensor) has also been developed.
  • an on-chip image sensor With an on-chip image sensor, a clear image can be obtained at a certain distance (for example, several tens of micrometers) above the image sensor, so there is no need for focusing work.
  • a liquid sample is dropped onto the image sensor several times using a micropipette or similar device, the sample is photographed, and the object to be observed (such as a microorganism) contained within the sample is observed from the image obtained.
  • the object to be observed such as a microorganism
  • the cover member of the image sensor according to the embodiment has a presser part that presses the surface of the liquid sample placed in the sample placement space above the image sensor included in the imaging device so that the surface of the liquid sample becomes flat. .
  • FIG. 1 is a diagram illustrating an example of the configuration of an entire system including a cover member of an image sensor according to an embodiment.
  • FIG. 2 is a diagram showing an example of the structure of the cover member 4 shown in FIG. 1 when viewed from above with the cover member 4 attached to the imaging device 1.
  • FIG. 3 is a diagram showing an example of the state of the sample S before the cover member 4 is attached to the imaging device 1.
  • FIG. 4 is a diagram showing an example of the state of the sample S after the cover member 4 is attached to the imaging device 1.
  • FIG. 5 is a diagram showing an example (part 1) of the structure of the cover member 4.
  • FIG. 6 is a diagram showing an example (part 2) of the structure of the cover member 4.
  • FIG. 1 is a diagram illustrating an example of the configuration of an entire system including a cover member of an image sensor according to an embodiment.
  • FIG. 2 is a diagram showing an example of the structure of the cover member 4 shown in FIG. 1 when viewed from above with the cover member 4 attached to the imaging
  • FIG. 7 is a diagram showing an example (part 3) of the structure of the cover member 4.
  • FIG. 8 is a diagram showing a longitudinal cross-sectional shape of the cover member 4 shown in FIG. 7.
  • FIG. 9 is a diagram showing an example of an image obtained from the image sensor 11 when photographing is performed without the cover member 4 attached.
  • FIG. 10 is a diagram showing an example of an image obtained from the image sensor 11 when photographing is performed with the cover member 4 attached.
  • FIG. 11 is a diagram showing an example of the structure of the cover member 4 according to the first modification example when attached to the imaging device 1 as viewed from above.
  • FIG. 12 is a diagram showing an example of the state of the sample S when the cover member 4 according to Modification Example 1 is attached to the imaging device 1.
  • FIG. 13 is a diagram showing an example of the state of the sample S when the cover member 4 according to Modification Example 2 is attached to the imaging device 1.
  • FIG. 14 is a diagram showing an example of an image of the sample S before drying.
  • FIG. 15 is a diagram showing an example of an image after drying the sample S.
  • FIG. 16 is a flowchart showing an example (part 1) of the method for observing the sample S.
  • FIG. 17 is a flowchart showing an example (part 2) of the method for observing the sample S.
  • FIG. 1 shows an example of the configuration of the entire system including the cover member of the image sensor according to the embodiment. Further, FIG. 2 shows an example of the structure of the cover member 4 shown in FIG. 1 when viewed from above in a state where it is attached to the imaging device 1.
  • FIG. 1 shows an imaging device 1, an information processing device 2, a water treatment facility 3, a cover member 4, and a lighting 5. At least the imaging device 1 and the cover member 4 constitute an observation system.
  • the imaging device 1 corresponds to an on-chip image sensor, and includes an image sensor 11 inside, a frame 1B arranged around the image sensor 11, and a liquid film on the image sensor 11 and the frame 1B.
  • a sample placement space 1A is provided so that the sample S can be placed therein.
  • the sample placement space 1A corresponds to a concave depression formed in a part of the upper part of the main body of the imaging device 1, and accommodates the liquid sample S.
  • the sample S is, for example, activated sludge water (sludge water) containing microorganisms, or treated water containing flocs.
  • the cover member 4 has a presser portion 4A that presses the surface of the liquid sample S placed in the sample placement space 1A so that the surface of the liquid sample S becomes flat.
  • the holding part 4A has a flat surface that comes into contact with the sample S.
  • At least the pressing portion 4A and the portion vertical to it of the cover member 4 are made of a material that transmits light.
  • a material that transmits light is made of a material that transmits light.
  • the cover member 4 is made of quartz glass or a resin such as COP (Cyclo-Olefin Polymer).
  • the shape of the cover member 4 is circular, but the shape of the cover member 4 is not limited to this example, and may be square, for example, as described later, or may have other shapes. It may be.
  • the illumination 5 emits light toward the cover member 4 from above the cover member 4 attached to the imaging device 1 .
  • the light from the illumination 5 passes through the cover member 4 and illuminates the sample S.
  • the lighting 5 is, for example, a white LED (Light Emitting Diode).
  • the present invention is not limited to this example, and any type of illumination may be used as long as it emits light that passes through the cover member 4.
  • the image sensor 11 photographs the sample S as a subject and outputs the photographed result as a two-dimensional image.
  • the imaging device 1 transmits image information obtained from the image sensor 11 to the information processing device 2 .
  • the information processing device 2 corresponds to a computer, and displays the information of the image transmitted from the imaging device 1 on a display device, and displays various observation objects included in the sample S shown in the image, for example. It analyzes microorganisms, flocs, etc. (hereinafter referred to as "microorganisms, etc.”), and transmits the analysis results to the water treatment facility 3.
  • the information processing device 2 determines the types of microorganisms contained in the sludge water and the amount per unit volume from the image, and transmits information indicating the determined types of microorganisms and the amount per unit volume to the water treatment facility 3 (for example, a sewage treatment plant). Also, for example, if the sample S is water to be treated that contains flocs, the information processing device 2 determines the size of the flocs contained in the water to be treated and the amount per unit volume, and transmits information indicating the determined size of the flocs and the amount per unit volume to the water treatment facility 3 (for example, a water purification plant).
  • the water treatment facility 3 controls a predetermined control target based on the information transmitted from the information processing device 2. For example, if the water treatment facility 3 is a sewage treatment plant, the amount of deaeration in a predetermined tank may be controlled depending on the type of microorganisms contained in the sludge water and the amount per unit volume. For example, if the water treatment facility 3 is a water treatment plant, the water is injected into a predetermined water treatment pond (for example, a mixing pond) depending on the size of flocs contained in the water to be treated and the amount per unit volume. control of drugs (e.g. flocculants);
  • drugs e.g. flocculants
  • FIG. 3 shows an example of the state of the sample S before the cover member 4 is attached to the imaging device 1.
  • FIG. 4 shows an example of the state of the sample S after the cover member 4 is attached to the imaging device 1.
  • the vertical length of the sample mounting space 1A shown in FIG. 3 (the depth from the upper surface of the imaging device 1 to the surface of the image sensor 11) is a, and the depth of field of the image sensor 11 is x.
  • a is, for example, 4 to 5 mm
  • x is, for example, several tens of ⁇ m.
  • a predetermined amount of the liquid sample S containing the observation target T is dropped into the sample mounting space 1A above the image sensor 11 using a micropipette or the like.
  • a part of the sample S may not fall within the depth of field x of the image sensor 11.
  • a clear image cannot be obtained from the image sensor 11.
  • the observation target T is a light microorganism or a microorganism that does not sink easily
  • the microorganism does not fall within the depth of field x of the image sensor 11 and appears blurred in the two-dimensional image of the image sensor 11. This makes it impossible to properly observe light microorganisms, etc.
  • the sample S may not be sufficiently spread over the entire surface of the image sensor. Moreover, the sample S with a rounded surface is likely to fluctuate. In such a state, a clear image cannot be obtained from the image sensor 11. For example, the peripheral image portion of the two-dimensional image of the image sensor 11 becomes unclear, making it impossible to properly observe the observation target T that should be reflected there.
  • the cover member 4 is attached to the imaging device 1. Specifically, the convex portion of the cover member 4 (that is, the holding portion 4A) enters the concave portion of the imaging device 1 (that is, the portion excluding the sample S in the sample mounting space 1A). At this time, a portion around the holding portion 4A (a portion of the surface of the cover member 4) rests on the hook portion 1C of the imaging device 1 (a portion of the upper surface of the imaging device 1).
  • the holding part 4A of the holding part 4A has a flat surface at the part that contacts the sample S, and presses the surface of the sample S so that the surface of the sample S becomes flat. At this time, the surface of the holding portion 4A in contact with the sample S is parallel to the surface of the image sensor 11.
  • the holding part 4A presses the surface of the sample S so that the sample S falls within the depth of field x of the image sensor 11.
  • the holding portion 4A has a vertical length ax that allows the sample S to fall within the depth of field x of the image sensor 11.
  • This "vertical length a-x of the holding part 4A" is the length obtained by subtracting the "depth of field x of the image sensor 11" from the aforementioned "vertical length a of the sample mounting space 1A". It is.
  • the cover member 4 pushes the surface of the sample S so that the surface of the sample S becomes flat.
  • the holding portion 4A has a cross-sectional shape and dimensions that match the horizontal cross-sectional shape and dimensions of the sample mounting space 1A.
  • the horizontal cross-sectional shape of the holding portion 4A is square and has approximately the same dimensions as the dimensions of m ⁇ m.
  • it is desirable that the shapes and dimensions of both are adjusted so that the holding portion 4A can fit comfortably into the sample placement space 1A without creating a gap.
  • cover member 4 (Various shapes of cover member 4)
  • the shape of the cover member 4 may be changed as appropriate depending on the structure of the imaging device 1 to which the cover member 4 is attached, and the environment and application in which the cover member 4 is used.
  • FIG. 8 shows a longitudinal cross-sectional shape of the cover member 4 shown in FIG. 7. As shown in FIG.
  • FIG. 5 shows a cover member 4 having a structure similar to that described in FIGS. 1 and 2. That is, the cover member 4 shown in FIG. 5 includes a rectangular prism-shaped structure 41 forming the holding part 4A, and a flat plate-shaped structure 42 having a surface that comes into contact with the hook part 1C of the imaging device 1 described above. including.
  • the flat structure 42 has a circular shape when viewed from above.
  • Such a circular structure 42 has no sharp corners, and can prevent hands from getting hurt when handling the cover member 4.
  • the cover member 4 shown in FIG. 6 has a different surface shape from the above-described imaging device 1. That is, the cover member 4 shown in FIG. 6 includes a rectangular prism-shaped structure 41 forming the holding part 4A, and a flat plate-shaped structure 43 having a surface that comes into contact with the hook part 1C of the imaging device 1 described above. including.
  • the flat structure 43 has a square shape when viewed from above.
  • Such a square structure 42 is relatively easy to manufacture and easy to handle during manufacture.
  • the cover member 4 shown in FIGS. 7 and 8 has a surface shape different from that of the imaging device 1 described above, for example, an imaging device in which the hook portion 1C is stepped in two steps (for example, the first hook portion 1C This is applied to an imaging device (imaging device) which has a second-stage hook portion 1D in addition to the hook portion 1D above the hook portion 1D. Note that an imaging device having this type of structure will be described later.
  • the cover member 4 shown in FIGS. 7 and 8 includes a rectangular prism-shaped structure 41 forming a holding part 4A, a cylindrical structure 44 having a surface that comes into contact with the first hook part 1C, and two parts. It includes a flat structure 45 having a surface that comes into contact with the hook portion 1D of the step.
  • the inside of the cylindrical structure 44 is hollow, and an empty space 46 is formed above the structure 41.
  • the flat structure 45 is viewed from above, there is a space 46 in the center surrounded by the cylindrical structure 44, and the structure 41 is located at the back of the space 46.
  • the structures 44 and 45 may be made of a material that does not transmit light.
  • the cover member 4 having such a structure can be used even when the image sensor 11 and the sample placement space 1A are provided deep from the upper surface of the imaging device. Further, since there is no need to increase the vertical thickness of the square columnar structure 41 forming the holding portion 4A, the clarity of the image is not reduced, and it is light and easy to carry.
  • the entire sample S is placed within the depth of field x of the image sensor 11.
  • the observation target T is light microorganisms or microorganisms that are difficult to sink, they fall within the depth of field x of the image sensor 11 and are clearly reflected in the two-dimensional image of the image sensor 11, so light microorganisms, etc. can be observed.
  • the sample S sufficiently spreads over the entire surface of the image sensor without being affected by surface tension, the image area at the periphery of the two-dimensional image of the image sensor 11 also becomes clear, and the observation target T reflected there also becomes clear. Can be observed appropriately. Further, since the sample S does not fluctuate, observation can be performed in a stable state.
  • FIG. 9 shows an example of an image obtained from the image sensor 11 when photographing is performed without the cover member 4 attached.
  • FIG. 10 shows an example of an image obtained from the image sensor 11 when photographing is performed with the cover member 4 attached.
  • FIG. 11 shows an example of the structure of the cover member 4 according to the first modification when it is attached to the imaging device 1 and viewed from above.
  • FIG. 12 shows an example of the state of the sample S when the cover member 4 according to the first modification is attached to the imaging device 1.
  • the cover member 4 according to the first modification has a drainage channel 4B through which a portion of the sample S that becomes surplus when the sample S is held down by the holding part 4A is discharged to the outside. We are prepared.
  • each drainage channel 4B is provided at the edge portion of the holding portion 4A and the portion that contacts the hook portion 1C. It is designed not to enter the area.
  • the excess sample S can be easily and efficiently discharged from each location when the cover member 4 is pushed down.
  • photographing of the sample S can be prevented from being adversely affected.
  • FIG. 13 shows an example of the state of the sample S when the cover member 4 according to Modification 2 is attached to the imaging device 1.
  • the structure of the cover member 4 shown in FIG. 13 corresponds to the structure of the cover member 4 explained in FIGS. 7 and 8.
  • the hook portion 1C has two steps in the shape of a step, as described above. That is, in addition to the first stage hook part 1C, there is a second stage hook part 1D above it.
  • the cover member 4 according to the second modification can be attached to the imaging device 1 of the type that has a plurality of hook portions. Further, as described above, since there is no need to increase the vertical thickness of the holding portion 4A, there is an advantage that the sharpness of the image is not reduced and it is light and easy to carry.
  • the sample S can be placed within the depth of field x of the image sensor 11, and a clear image can be obtained.
  • the sample S can be made to fall within the range of the depth of field x of the image sensor 11, and a clear image can be obtained.
  • FIG. 14 shows an example of an image of sample S before drying.
  • FIG. 15 shows an example of an image after drying the sample S.
  • a cover member 4 is used here in order to obtain a clear image.
  • step S11 a specified amount of the sample S is measured (step S11), and the specified amount of the sample S is dropped into the sample mounting space 1A above the image sensor 11 (step S12).
  • the cover member 4 is placed on the imaging device 1, and the surface of the sample S placed in the sample placement space 1A on the image sensor 11 is flattened (step S13).
  • the illumination 5 is installed above the cover member 4 so that the light from the illumination 5 passes through the cover member 4 and illuminates the sample S (step S14).
  • Step S15 the sample S is photographed and the image output from the image sensor 11 is acquired by the information processing device 2, the acquired image is displayed on the display device, and microorganisms, etc. contained in the sample S are observed from the image.
  • the sample S is dried in order to obtain a clear image.
  • a specified amount of sample S is measured (step S21), and the specified amount of sample S is dropped into the sample placement space 1A above the image sensor 11 (step S22).
  • the illumination 5 is installed above the cover member 4 so that the light from the illumination 5 illuminates the sample S (step S24).
  • Step S25 the sample S is photographed and the image output from the image sensor 11 is acquired by the information processing device 2, the acquired image is displayed on the display device, and microorganisms, etc. contained in the sample S are observed from the image.

Abstract

An image sensor cover member (4) according to an embodiment comprises a pressing part (4A) that presses the surface of a liquid sample (S) placed in a sample placement space (1A) above an image sensor (11) included in an imaging device (1) such that the surface of the sample (S) becomes flat.

Description

イメージセンサーのカバー部材、観察システム、および観察方法Image sensor cover member, observation system, and observation method
　本発明の実施形態は、イメージセンサーのカバー部材、観察システム、および観察方法に関する。 Embodiments of the present invention relate to a cover member for an image sensor, an observation system, and an observation method.
　近年、安価で性能の良いイメージセンサーが開発されており、これらを使って微生物や細胞を観察する方法が知られている。 In recent years, inexpensive and high-performance image sensors have been developed, and methods using these to observe microorganisms and cells are known.
　さらに、オンチップイメージセンサーと呼ばれる撮像装置（センサー上に直接試料を乗せて画像を取得できるようにしたもの）も開発されている。オンチップイメージセンサーでは、イメージセンサー上のある一定距離（例えば数１０μｍ）で明瞭な画像が得られるため、焦点合わせの作業が不要となる。 Furthermore, an imaging device called an on-chip image sensor (a device that can capture images by placing a sample directly on the sensor) has also been developed. With an on-chip image sensor, a clear image can be obtained at a certain distance (for example, several tens of micrometers) above the image sensor, so there is no need for focusing work.
　オンチップイメージセンサーと呼ばれる撮像装置では、例えば液状の試料をマイクロピペット等でイメージセンサーの上に何回か滴下した上で、試料の撮影を行い、撮影で得られた画像から試料の中に含まれる観察対象物（微生物等）を観察する。その際に、明瞭な画像が得られないことがある。その理由としては、以下が挙げられる。 In imaging devices known as on-chip image sensors, for example, a liquid sample is dropped onto the image sensor several times using a micropipette or similar device, the sample is photographed, and the object to be observed (such as a microorganism) contained within the sample is observed from the image obtained. In such cases, it is sometimes difficult to obtain a clear image. The reasons for this include the following:
　・軽い観察対象物の場合、当該観察対象物がイメージセンサーの被写界深度の範囲内に収まらず、その観察対象物が画像では不鮮明に映る。 ・If the object to be observed is light, it will not fit within the depth of field of the image sensor, and the object will appear unclear in the image.
　・観察対象物の種類によって沈み方が異なり、沈ににくい観察対象物は、イメージセンサーの被写界深度の範囲内に収まらず、その観察対象物が画像では不鮮明に映る。 ・The way the object sinks differs depending on the type of object being observed, and objects that are difficult to sink will not fall within the depth of field of the image sensor, making the object appear unclear in the image.
　・滴下した試料が表面張力等の影響でイメージセンサー全面に十分に行きわたっていない。 ・The dropped sample does not fully cover the entire surface of the image sensor due to surface tension, etc.
　このようなことから、イメージセンサーの上に載置される試料について明瞭な画像を得ることを可能にする、イメージセンサーのカバー部材、観察システム、および観察方法の提示が望まれる。 For this reason, it is desired to provide an image sensor cover member, an observation system, and an observation method that make it possible to obtain a clear image of a sample placed on the image sensor.
　実施形態によるイメージセンサーのカバー部材は、撮像装置に備えられるイメージセンサーの上の試料載置空間に載置される液状の試料の表面が平らになるように当該試料の表面を押える押え部を有する。 The cover member of the image sensor according to the embodiment has a presser part that presses the surface of the liquid sample placed in the sample placement space above the image sensor included in the imaging device so that the surface of the liquid sample becomes flat. .
図１は、実施形態のイメージセンサーのカバー部材を含むシステム全体の構成の一例を示す図である。FIG. 1 is a diagram illustrating an example of the configuration of an entire system including a cover member of an image sensor according to an embodiment. 図２は、図１中に示されるカバー部材４を、撮像装置１に取り付けた状態で上から見たときの構造の一例を示す図である。FIG. 2 is a diagram showing an example of the structure of the cover member 4 shown in FIG. 1 when viewed from above with the cover member 4 attached to the imaging device 1. As shown in FIG. 図３は、カバー部材４を撮像装置１に取り付ける前の試料Ｓの状態の一例を示す図である。FIG. 3 is a diagram showing an example of the state of the sample S before the cover member 4 is attached to the imaging device 1. 図４は、カバー部材４を撮像装置１に取り付けた後の試料Ｓの状態の一例を示す図である。FIG. 4 is a diagram showing an example of the state of the sample S after the cover member 4 is attached to the imaging device 1. 図５は、カバー部材４の構造の例（その１）を示す図である。FIG. 5 is a diagram showing an example (part 1) of the structure of the cover member 4. 図６は、カバー部材４の構造の例（その２）を示す図である。FIG. 6 is a diagram showing an example (part 2) of the structure of the cover member 4. 図７は、カバー部材４の構造の例（その３）を示す図である。FIG. 7 is a diagram showing an example (part 3) of the structure of the cover member 4. 図８は、図７に示されるカバー部材４の縦断面形状を示す図である。FIG. 8 is a diagram showing a longitudinal cross-sectional shape of the cover member 4 shown in FIG. 7. As shown in FIG. 図９は、カバー部材４を取り付けない状態で撮影を行った場合にイメージセンサー１１から得られる画像の一例を示す図である。FIG. 9 is a diagram showing an example of an image obtained from the image sensor 11 when photographing is performed without the cover member 4 attached. 図１０は、カバー部材４を取り付けた状態で撮影を行った場合にイメージセンサー１１から得られる画像の一例を示す図である。FIG. 10 is a diagram showing an example of an image obtained from the image sensor 11 when photographing is performed with the cover member 4 attached. 図１１は、変形例１に係るカバー部材４を撮像装置１に取り付けた状態で上から見たときの構造の一例を示す図である。FIG. 11 is a diagram showing an example of the structure of the cover member 4 according to the first modification example when attached to the imaging device 1 as viewed from above. 図１２は、変形例１に係るカバー部材４を撮像装置１に取り付けたときの試料Ｓの状態の一例を示す図である。FIG. 12 is a diagram showing an example of the state of the sample S when the cover member 4 according to Modification Example 1 is attached to the imaging device 1. 図１３は、変形例２に係るカバー部材４を撮像装置１に取り付けたときの試料Ｓの状態の一例を示す図である。FIG. 13 is a diagram showing an example of the state of the sample S when the cover member 4 according to Modification Example 2 is attached to the imaging device 1. 図１４は、試料Ｓを乾燥する前の画像の一例を示す図である。FIG. 14 is a diagram showing an example of an image of the sample S before drying. 図１５は、試料Ｓを乾燥した後の画像の一例を示す図である。FIG. 15 is a diagram showing an example of an image after drying the sample S. 図１６は、試料Ｓの観察方法の例（その１）を示すフローチャートである。FIG. 16 is a flowchart showing an example (part 1) of the method for observing the sample S. 図１７は、試料Ｓの観察方法の例（その２）を示すフローチャートである。FIG. 17 is a flowchart showing an example (part 2) of the method for observing the sample S.
実施形態Embodiment
　以下、図面を参照して、実施の形態について説明する。 Hereinafter, embodiments will be described with reference to the drawings.
　（システム構成）
　図１に、実施形態のイメージセンサーのカバー部材を含むシステム全体の構成の一例を示す。また、図２に、図１中に示されるカバー部材４を、撮像装置１に取り付けた状態で上から見たときの構造の一例を示す。 (System configuration)
FIG. 1 shows an example of the configuration of the entire system including the cover member of the image sensor according to the embodiment. Further, FIG. 2 shows an example of the structure of the cover member 4 shown in FIG. 1 when viewed from above in a state where it is attached to the imaging device 1.
　図１には、撮像装置１、情報処理装置２、水処理施設３、カバー部材４、および照明５が示されている。少なくとも撮像装置１およびカバー部材４は、観察システムを構成する。 FIG. 1 shows an imaging device 1, an information processing device 2, a water treatment facility 3, a cover member 4, and a lighting 5. At least the imaging device 1 and the cover member 4 constitute an observation system.
　撮像装置１は、オンチップイメージセンサーに相当するものであり、内部にイメージセンサー１１を備えるとともに、イメージセンサー１１の周囲に配置されるフレーム１Ｂを備え、イメージセンサー１１およびフレーム１Ｂの上に液状の試料Ｓを載置できるよう試料載置空間１Ａを備える。 The imaging device 1 corresponds to an on-chip image sensor, and includes an image sensor 11 inside, a frame 1B arranged around the image sensor 11, and a liquid film on the image sensor 11 and the frame 1B. A sample placement space 1A is provided so that the sample S can be placed therein.
　試料載置空間１Ａは、撮像装置１の本体の上側の一部に凹状に形成された窪みに相当し、液状の試料Ｓを収容する。試料Ｓは、例えば、微生物を含む活性汚泥の水（汚泥水）、もしくは、フロックを含む処理水などである。 The sample placement space 1A corresponds to a concave depression formed in a part of the upper part of the main body of the imaging device 1, and accommodates the liquid sample S. The sample S is, for example, activated sludge water (sludge water) containing microorganisms, or treated water containing flocs.
　カバー部材４は、試料載置空間１Ａに載置される液状の試料Ｓの表面が平らになるように当該試料Ｓの表面を押える押え部４Ａを有する。押え部４Ａは、試料Ｓと接する平らな面を有する。 The cover member 4 has a presser portion 4A that presses the surface of the liquid sample S placed in the sample placement space 1A so that the surface of the liquid sample S becomes flat. The holding part 4A has a flat surface that comes into contact with the sample S.
　カバー部材４のうち、少なくとも押え部４Ａおよびその鉛直方向にある部分は、光を透過する材質で形成されている。ここでは、押え部４Ａを含めてカバー部材４全体が同一の材質で形成されている例を示す。カバー部材４は、石英ガラス、もしくはＣＯＰ（Cyclo-Olefin Polymer）などの樹脂である。 At least the pressing portion 4A and the portion vertical to it of the cover member 4 are made of a material that transmits light. Here, an example is shown in which the entire cover member 4, including the pressing portion 4A, is made of the same material. The cover member 4 is made of quartz glass or a resin such as COP (Cyclo-Olefin Polymer).
　カバー部材４を上から見ると、図２に示されるように、イメージセンサー１１およびフレーム１Ｂが透けて見える。なお、図２の例では、カバー部材４の形状が円形になっているが、カバー部材４の形状はこの例に限らず、例えば後述するように正方形であってもよいし、それ以外の形であってもよい。 When the cover member 4 is viewed from above, the image sensor 11 and the frame 1B can be seen through it, as shown in FIG. In the example of FIG. 2, the shape of the cover member 4 is circular, but the shape of the cover member 4 is not limited to this example, and may be square, for example, as described later, or may have other shapes. It may be.
　照明５は、撮像装置１に取り付けられるカバー部材４の上方から、カバー部材４へ向けて光を放つ。照明５の光は、カバー部材４を透過し、試料Ｓを照らす。照明５は、例えば白色ＬＥＤ（Light Emitting Diode）である。ただし、この例に限らず、カバー部材４を透過する光を放つものであれば、どのような種類の照明を採用してもよい。 The illumination 5 emits light toward the cover member 4 from above the cover member 4 attached to the imaging device 1 . The light from the illumination 5 passes through the cover member 4 and illuminates the sample S. The lighting 5 is, for example, a white LED (Light Emitting Diode). However, the present invention is not limited to this example, and any type of illumination may be used as long as it emits light that passes through the cover member 4.
　イメージセンサー１１は、試料Ｓを被写体として撮影し、撮影した結果を二次元画像として出力する。撮像装置１は、イメージセンサー１１から得られる画像の情報を情報処理装置２へ伝送する。 The image sensor 11 photographs the sample S as a subject and outputs the photographed result as a two-dimensional image. The imaging device 1 transmits image information obtained from the image sensor 11 to the information processing device 2 .
　情報処理装置２は、コンピュータに相当するものであり、撮像装置１から伝送されてくる画像の情報を表示装置に表示したり、画像に映っている試料Ｓに含まれる各種の観察対象物、例えば微生物やフロックなど（以降、「微生物等」と称す。）を解析したり、その解析結果を水処理施設３に伝送したりする。 The information processing device 2 corresponds to a computer, and displays the information of the image transmitted from the imaging device 1 on a display device, and displays various observation objects included in the sample S shown in the image, for example. It analyzes microorganisms, flocs, etc. (hereinafter referred to as "microorganisms, etc."), and transmits the analysis results to the water treatment facility 3.
　情報処理装置２は、例えば、試料Ｓが汚泥水である場合には、画像から汚泥水に含まれる各種の微生物の種類や単位容積当たりの量を判定したり、判定した微生物の種類や単位容積当たりの量を示す情報を水処理施設３（例えば、下水処理場）に伝送したりする。また、情報処理装置２は、例えば、試料Ｓがフロックを含む被処理水である場合には、被処理水に含まれるフロックの大きさや単位容積当たりの量を判定したり、判定したフロックの大きさや単位容積当たりの量を示す情報を水処理施設３（例えば、浄水処理場）に伝送したりする。 For example, if the sample S is sludge water, the information processing device 2 determines the types of microorganisms contained in the sludge water and the amount per unit volume from the image, and transmits information indicating the determined types of microorganisms and the amount per unit volume to the water treatment facility 3 (for example, a sewage treatment plant). Also, for example, if the sample S is water to be treated that contains flocs, the information processing device 2 determines the size of the flocs contained in the water to be treated and the amount per unit volume, and transmits information indicating the determined size of the flocs and the amount per unit volume to the water treatment facility 3 (for example, a water purification plant).
　水処理施設３は、情報処理装置２から伝送される情報に基づいて、所定の制御対象を制御する。例えば、水処理施設３が下水処理場の場合は、汚泥水に含まれる微生物の種類や単位容積当たりの量に応じて所定の槽の脱気量を制御したりする。また、例えば、水処理施設３が浄水処理場の場合は、被処理水に含まれるフロックの大きさや単位容積当たりの量に応じて所定の水処理用の池（例えば、混和池）に注入する薬剤（例えば、凝集剤）を制御したりする。 The water treatment facility 3 controls a predetermined control target based on the information transmitted from the information processing device 2. For example, if the water treatment facility 3 is a sewage treatment plant, the amount of deaeration in a predetermined tank may be controlled depending on the type of microorganisms contained in the sludge water and the amount per unit volume. For example, if the water treatment facility 3 is a water treatment plant, the water is injected into a predetermined water treatment pond (for example, a mixing pond) depending on the size of flocs contained in the water to be treated and the amount per unit volume. control of drugs (e.g. flocculants);
　（カバー部材４の構造的特徴）
　図３に、カバー部材４を撮像装置１に取り付ける前の試料Ｓの状態の一例を示す。また、図４に、カバー部材４を撮像装置１に取り付けた後の試料Ｓの状態の一例を示す。 (Structural characteristics of cover member 4)
FIG. 3 shows an example of the state of the sample S before the cover member 4 is attached to the imaging device 1. Further, FIG. 4 shows an example of the state of the sample S after the cover member 4 is attached to the imaging device 1.
　図３に示される試料載置空間１Ａの鉛直方向の長さ（撮像装置１の上側表面からイメージセンサー１１表面までの深さ）をａとし、イメージセンサー１１の被写界深度をｘとする。ａは例えば４～５ｍｍであり、ｘは例えば数１０μｍである。 The vertical length of the sample mounting space 1A shown in FIG. 3 (the depth from the upper surface of the imaging device 1 to the surface of the image sensor 11) is a, and the depth of field of the image sensor 11 is x. a is, for example, 4 to 5 mm, and x is, for example, several tens of μm.
　図３に示されるように、観察対象物Ｔを含む液状の試料Ｓは、イメージセンサー１１の上の試料載置空間１Ａにマイクロピペット等を用いて所定量が滴下される。 As shown in FIG. 3, a predetermined amount of the liquid sample S containing the observation target T is dropped into the sample mounting space 1A above the image sensor 11 using a micropipette or the like.
　このとき、例えば試料Ｓの一部がイメージセンサー１１の被写界深度ｘの範囲に収まらない状態になる場合がある。そのような状態では、イメージセンサー１１からは明瞭な画像が得られない。例えば、観察対象物Ｔが軽い微生物等や沈みにくい微生物等である場合、その微生物等がイメージセンサー１１の被写界深度ｘの範囲内に収まらず、イメージセンサー１１の二次元画像ではぼやけた状態で映るため、軽い微生物等を適切に観察することができない。 At this time, for example, a part of the sample S may not fall within the depth of field x of the image sensor 11. In such a state, a clear image cannot be obtained from the image sensor 11. For example, if the observation target T is a light microorganism or a microorganism that does not sink easily, the microorganism does not fall within the depth of field x of the image sensor 11 and appears blurred in the two-dimensional image of the image sensor 11. This makes it impossible to properly observe light microorganisms, etc.
　また、例えば表面張力の影響で、試料Ｓがイメージセンサー全面に十分に行きわたらない状態になる場合がある。また、表面に丸みがある試料Ｓは、揺らぎやすい。そのような状態では、イメージセンサー１１からは明瞭な画像が得られない。例えば、イメージセンサー１１の二次元画像の周縁部の画像部分が不鮮明となり、そこに映っているはずの観察対象物Ｔを適切に観察することができない。 Furthermore, for example, due to the influence of surface tension, the sample S may not be sufficiently spread over the entire surface of the image sensor. Moreover, the sample S with a rounded surface is likely to fluctuate. In such a state, a clear image cannot be obtained from the image sensor 11. For example, the peripheral image portion of the two-dimensional image of the image sensor 11 becomes unclear, making it impossible to properly observe the observation target T that should be reflected there.
　図４に示されるように、カバー部材４は、撮像装置１に取り付けられ。具体的には、カバー部材４の凸状の部分（すなわち、押え部４Ａ）が、撮像装置１の凹状の部分（すなわち、試料載置空間１Ａ内の試料Ｓを除いた部分）に入る。このとき、撮像装置１の引っ掛け部１Ｃ（撮像装置１の上側の表面の一部）に、押え部４Ａの周囲の部分（カバー部材４の表面の一部）が乗る形となる。 As shown in FIG. 4, the cover member 4 is attached to the imaging device 1. Specifically, the convex portion of the cover member 4 (that is, the holding portion 4A) enters the concave portion of the imaging device 1 (that is, the portion excluding the sample S in the sample mounting space 1A). At this time, a portion around the holding portion 4A (a portion of the surface of the cover member 4) rests on the hook portion 1C of the imaging device 1 (a portion of the upper surface of the imaging device 1).
　図４から理解できるように、押え部４Ａの押え部４Ａは、試料Ｓと接する部分が平らな面になっており、試料Ｓの表面が平らになるように当該試料Ｓの表面を押える。このとき、押え部４Ａが試料Ｓと接する面は、イメージセンサー１１の表面と平行である。 As can be understood from FIG. 4, the holding part 4A of the holding part 4A has a flat surface at the part that contacts the sample S, and presses the surface of the sample S so that the surface of the sample S becomes flat. At this time, the surface of the holding portion 4A in contact with the sample S is parallel to the surface of the image sensor 11.
　押え部４Ａは、イメージセンサー１１の被写界深度ｘの範囲内に試料Ｓが収まるように当該試料Ｓの表面を押える。具体的には、押え部４Ａは、イメージセンサー１１の被写界深度ｘの範囲内に試料Ｓが収まるようにする鉛直方向の長さａ－ｘを有する。この「押え部４Ａの鉛直方向の長さａ－ｘ」は、前述した「試料載置空間１Ａの鉛直方向の長さａ」から「イメージセンサー１１の被写界深度ｘ」を差し引いた長さである。これにより、カバー部材４は、試料Ｓの表面が平らになるように当該試料Ｓの表面を押すことになる。 The holding part 4A presses the surface of the sample S so that the sample S falls within the depth of field x of the image sensor 11. Specifically, the holding portion 4A has a vertical length ax that allows the sample S to fall within the depth of field x of the image sensor 11. This "vertical length a-x of the holding part 4A" is the length obtained by subtracting the "depth of field x of the image sensor 11" from the aforementioned "vertical length a of the sample mounting space 1A". It is. Thereby, the cover member 4 pushes the surface of the sample S so that the surface of the sample S becomes flat.
　また、押え部４Ａは、図２に示されるように、試料載置空間１Ａの水平方向の断面形状および寸法に合わせた断面形状および寸法を有する。例えば、試料載置空間１Ａの水平方向の断面形状が正方形でｍ×ｍの寸法を有する場合、押え部４Ａの水平方向の断面形状が正方形でｍ×ｍの寸法とほぼ同じ寸法を有する。ただし、双方の形状や寸法は、押え部４Ａが試料載置空間１Ａに無理なく且つ隙間を生ずることなく入るように調整されていることが望ましい。 Furthermore, as shown in FIG. 2, the holding portion 4A has a cross-sectional shape and dimensions that match the horizontal cross-sectional shape and dimensions of the sample mounting space 1A. For example, when the sample placement space 1A has a square cross-sectional shape in the horizontal direction and has dimensions of m×m, the horizontal cross-sectional shape of the holding portion 4A is square and has approximately the same dimensions as the dimensions of m×m. However, it is desirable that the shapes and dimensions of both are adjusted so that the holding portion 4A can fit comfortably into the sample placement space 1A without creating a gap.
　（カバー部材４の様々な形状）
　カバー部材４の形状は、カバー部材４が取り付けられる撮像装置１の構造や、カバー部材４が使用される環境や用途に応じて適宜変えてもよい。 (Various shapes of cover member 4)
The shape of the cover member 4 may be changed as appropriate depending on the structure of the imaging device 1 to which the cover member 4 is attached, and the environment and application in which the cover member 4 is used.
　図５、図６、および図７に、カバー部材４の様々な構造の例を斜視図にて示す。図８は、図７に示されるカバー部材４の縦断面形状を示したものである。 Examples of various structures of the cover member 4 are shown in perspective views in FIGS. 5, 6, and 7. FIG. 8 shows a longitudinal cross-sectional shape of the cover member 4 shown in FIG. 7. As shown in FIG.
　図５は、図１及び図２で説明したカバー部材４と同様の構造を有するものを示している。すなわち、図５に示されるカバー部材４は、押え部４Ａを形成している四角柱状の構造物４１と、前述した撮像装置１の引っ掛け部１Ｃに当接する面がある平板状の構造物４２とを含む。平板状の構造物４２は、上から見た形が円形である。 FIG. 5 shows a cover member 4 having a structure similar to that described in FIGS. 1 and 2. That is, the cover member 4 shown in FIG. 5 includes a rectangular prism-shaped structure 41 forming the holding part 4A, and a flat plate-shaped structure 42 having a surface that comes into contact with the hook part 1C of the imaging device 1 described above. including. The flat structure 42 has a circular shape when viewed from above.
　このような円形の構造物４２は、鋭い角の部分が無く、カバー部材４を扱う上で手などが傷つくことを防止することができる。 Such a circular structure 42 has no sharp corners, and can prevent hands from getting hurt when handling the cover member 4.
　図６に示されるカバー部材４は、前述した撮像装置１と表面形状が異なる。すなわち、図６に示されるカバー部材４は、押え部４Ａを形成している四角柱状の構造物４１と、前述した撮像装置１の引っ掛け部１Ｃに当接する面がある平板状の構造物４３とを含む。平板状の構造物４３は、上から見た形が正方形である。 The cover member 4 shown in FIG. 6 has a different surface shape from the above-described imaging device 1. That is, the cover member 4 shown in FIG. 6 includes a rectangular prism-shaped structure 41 forming the holding part 4A, and a flat plate-shaped structure 43 having a surface that comes into contact with the hook part 1C of the imaging device 1 described above. including. The flat structure 43 has a square shape when viewed from above.
　このような正方形の構造物４２は、比較的製造しやすく、製造上の取り扱いもしやすい。 Such a square structure 42 is relatively easy to manufacture and easy to handle during manufacture.
　図７及び図８に示されるカバー部材４は、前述した撮像装置１と表面形状が異なるもの、例えば、引っ掛け部１Ｃが階段状に二段になっている撮像装置（例えば一段目の引っ掛け部１Ｃに加え、更にその上側に二段目の引っ掛け部１Ｄがある撮像装置）に適用される。なお、この種の構造の撮像装置については後で述べる。 The cover member 4 shown in FIGS. 7 and 8 has a surface shape different from that of the imaging device 1 described above, for example, an imaging device in which the hook portion 1C is stepped in two steps (for example, the first hook portion 1C This is applied to an imaging device (imaging device) which has a second-stage hook portion 1D in addition to the hook portion 1D above the hook portion 1D. Note that an imaging device having this type of structure will be described later.
　図７及び図８に示されるカバー部材４は、押え部４Ａを形成している四角柱状の構造物４１と、一段目の引っ掛け部１Ｃに当接する面がある筒状の構造物４４と、二段目の引っ掛け部１Ｄに当接する面がある平板状の構造物４５とを含む。筒状の構造物４４の内側は空洞になっており、構造物４１の上方には何もない空間４６が形成されている。平板状の構造物４５は、上から見ると、その中央に筒状の構造物４４で囲まれた空間４６があり、その奥に構造物４１がある。なお、構造物４４，４５の材質は、光を透過しない材質であってもよい。 The cover member 4 shown in FIGS. 7 and 8 includes a rectangular prism-shaped structure 41 forming a holding part 4A, a cylindrical structure 44 having a surface that comes into contact with the first hook part 1C, and two parts. It includes a flat structure 45 having a surface that comes into contact with the hook portion 1D of the step. The inside of the cylindrical structure 44 is hollow, and an empty space 46 is formed above the structure 41. When the flat structure 45 is viewed from above, there is a space 46 in the center surrounded by the cylindrical structure 44, and the structure 41 is located at the back of the space 46. Note that the structures 44 and 45 may be made of a material that does not transmit light.
　このような構造のカバー部材４は、イメージセンサー１１および試料載置空間１Ａが撮像装置上側表面から深いところに設けられている場合にも対応することができる。また、押え部４Ａを形成している四角柱状の構造物４１の鉛直方向の厚みを増やす必要がないため、画像の鮮明度を低減させることがなく、また、軽くて持ち運びがしやすい。 The cover member 4 having such a structure can be used even when the image sensor 11 and the sample placement space 1A are provided deep from the upper surface of the imaging device. Further, since there is no need to increase the vertical thickness of the square columnar structure 41 forming the holding portion 4A, the clarity of the image is not reduced, and it is light and easy to carry.
　（カバー部材４の利点）
　カバー部材４の押え部４Ａを図４で示したような構造とすることにより、試料Ｓの全てがイメージセンサー１１の被写界深度ｘの範囲に収まる状態になる。例えば、観察対象物Ｔが軽い微生物等や沈みにくい微生物等も、イメージセンサー１１の被写界深度ｘの範囲内に収まり、イメージセンサー１１の二次元画像に明瞭に映るため、軽い微生物等を適切に観察することができる。また、表面張力の影響を受けず、試料Ｓがイメージセンサー全面に十分に行きわたるため、イメージセンサー１１の二次元画像の周縁部の画像部分も鮮明となり、そこに映っている観察対象物Ｔも適切に観察することができる。また、試料Ｓの揺らぎも生じないため、安定した状態で観察を行うことができる。 (Advantages of cover member 4)
By configuring the holding portion 4A of the cover member 4 as shown in FIG. 4, the entire sample S is placed within the depth of field x of the image sensor 11. For example, even if the observation target T is light microorganisms or microorganisms that are difficult to sink, they fall within the depth of field x of the image sensor 11 and are clearly reflected in the two-dimensional image of the image sensor 11, so light microorganisms, etc. can be observed. In addition, since the sample S sufficiently spreads over the entire surface of the image sensor without being affected by surface tension, the image area at the periphery of the two-dimensional image of the image sensor 11 also becomes clear, and the observation target T reflected there also becomes clear. Can be observed appropriately. Further, since the sample S does not fluctuate, observation can be performed in a stable state.
　図９に、カバー部材４を取り付けない状態で撮影を行った場合にイメージセンサー１１から得られる画像の一例を示す。また、図１０に、カバー部材４を取り付けた状態で撮影を行った場合にイメージセンサー１１から得られる画像の一例を示す。 FIG. 9 shows an example of an image obtained from the image sensor 11 when photographing is performed without the cover member 4 attached. Further, FIG. 10 shows an example of an image obtained from the image sensor 11 when photographing is performed with the cover member 4 attached.
　カバー部材４を取り付けない場合は、図９に示されるように、軽い微生物等や沈みにくい微生物等は、イメージセンサー１１の被写界深度ｘの範囲内に収まらず、ぼやけた状態で映っている。そのため、軽い微生物等を適切に観察することができない。 If the cover member 4 is not attached, as shown in FIG. 9, light microorganisms or microorganisms that do not sink easily will not fall within the depth of field x of the image sensor 11 and will appear blurry. . Therefore, light microorganisms and the like cannot be properly observed.
　一方、カバー部材４を取り付けた場合は、図１０に示されるように、軽い微生物等や沈みにくい微生物等も含め、全ての微生物等は、イメージセンサー１１の被写界深度ｘの範囲内に収まっているため、殆どの微生物等の形状や大きさを確認することができ、それらを適切に観察することができる。 On the other hand, when the cover member 4 is attached, all microorganisms, etc., including light microorganisms and microorganisms that do not sink, are within the depth of field Therefore, it is possible to confirm the shape and size of most microorganisms, and to observe them appropriately.
　（カバー部材４の変形例１）
　次に、図１～図４で説明したカバー部材４の変形例１について説明する。 (Modification 1 of cover member 4)
Next, a first modification of the cover member 4 described in FIGS. 1 to 4 will be described.
　図１１に、変形例１に係るカバー部材４を撮像装置１に取り付けた状態で上から見たときの構造の一例を示す。また、図１２に、変形例１に係るカバー部材４を撮像装置１に取り付けたときの試料Ｓの状態の一例を示す。 FIG. 11 shows an example of the structure of the cover member 4 according to the first modification when it is attached to the imaging device 1 and viewed from above. Also, FIG. 12 shows an example of the state of the sample S when the cover member 4 according to the first modification is attached to the imaging device 1.
　図１１および図１２に示されるように、変形例１に係るカバー部材４は、試料Ｓが押え部４Ａにより押えられたときに余剰となる試料Ｓの一部を外部に排出させる排水路４Ｂを備えている。 As shown in FIGS. 11 and 12, the cover member 4 according to the first modification has a drainage channel 4B through which a portion of the sample S that becomes surplus when the sample S is held down by the holding part 4A is discharged to the outside. We are prepared.
　図１１に示されるように、カバー部材４を上から見ると、排水路４Ｂは複数カ所に設けられている。また、図１２に示されるように、カバー部材４を横から見ると、各排水路４Ｂは、押え部４Ａの縁の部分および引っ掛け部１Ｃと当たる部分に設けられており、イメージセンサー１１の撮影領域に入らないようになっている。 As shown in FIG. 11, when the cover member 4 is viewed from above, drainage channels 4B are provided at multiple locations. Further, as shown in FIG. 12, when the cover member 4 is viewed from the side, each drainage channel 4B is provided at the edge portion of the holding portion 4A and the portion that contacts the hook portion 1C. It is designed not to enter the area.
　排水路４Ｂを図１１のように各カ所に分散して設けることにより、カバー部材４を押し下げた際に各カ所から余剰となる試料Ｓを無理なく効率的に排出させることができる。また、図１２に示されるようにイメージセンサー１１の撮影領域に入らないように設けることにより、試料Ｓの撮影に悪影響を与えずに済む。 By providing the drainage channels 4B in different locations as shown in FIG. 11, the excess sample S can be easily and efficiently discharged from each location when the cover member 4 is pushed down. In addition, by providing it so as not to enter the photographing area of the image sensor 11 as shown in FIG. 12, photographing of the sample S can be prevented from being adversely affected.
　（カバー部材４の変形例２）
　次に、図１～図４で説明したカバー部材４の変形例２について説明する。 (Modification 2 of cover member 4)
Next, a second modification of the cover member 4 described in FIGS. 1 to 4 will be described.
　図１３に、変形例２に係るカバー部材４を撮像装置１に取り付けたときの試料Ｓの状態の一例を示す。図１３に示されるカバー部材４の構造は、図７及び図８で説明したカバー部材４の構造に相当する。また、このカバー部材４が適用される撮像装置１の構造は、前述したように引っ掛け部１Ｃが階段状に二段になっている。すなわち、一段目の引っ掛け部１Ｃに加え、更にその上側に二段目の引っ掛け部１Ｄがある。 FIG. 13 shows an example of the state of the sample S when the cover member 4 according to Modification 2 is attached to the imaging device 1. The structure of the cover member 4 shown in FIG. 13 corresponds to the structure of the cover member 4 explained in FIGS. 7 and 8. Further, in the structure of the imaging device 1 to which this cover member 4 is applied, the hook portion 1C has two steps in the shape of a step, as described above. That is, in addition to the first stage hook part 1C, there is a second stage hook part 1D above it.
　このように、変形例２に係るカバー部材４は、複数段の引っ掛け部を有するタイプの撮像装置１に取り付けることが可能である。また、前述したように、押え部４Ａの鉛直方向の厚みを増やす必要がないため、画像の鮮明度を低減させることがなく、また、軽くて持ち運びがしやすいという利点がある。 In this way, the cover member 4 according to the second modification can be attached to the imaging device 1 of the type that has a plurality of hook portions. Further, as described above, since there is no need to increase the vertical thickness of the holding portion 4A, there is an advantage that the sharpness of the image is not reduced and it is light and easy to carry.
　このように、上述した構造のカバー部材４を使用することにより、イメージセンサー１１の被写界深度ｘの範囲内に試料Ｓが収まるようにすることができ、鮮明な画像を得ることができる。一方、以下に説明する方法によっても、イメージセンサー１１の被写界深度ｘの範囲内に試料Ｓが収まるようにすることができ、鮮明な画像を得ることができる。 In this way, by using the cover member 4 having the above-described structure, the sample S can be placed within the depth of field x of the image sensor 11, and a clear image can be obtained. On the other hand, also by the method described below, the sample S can be made to fall within the range of the depth of field x of the image sensor 11, and a clear image can be obtained.
　（試料Ｓの乾燥）
　カバーを取り付ける前の撮像装置１においては、図３で説明したように、試料Ｓの一部がイメージセンサー１１の被写界深度ｘの範囲に収まらない状態になる場合があり、そのような状態では、イメージセンサー１１からは明瞭な画像が得られない。そこで、例えばカバー部材４を取り付けずに、当該試料Ｓを乾燥させる処理を行う。これにより、軽い微生物等や沈みにくい微生物等も含め、全ての微生物等は、イメージセンサー１１のすぐ上の位置に集まる（被写界深度ｘの範囲内に入る）ため、殆どの微生物等の形状や大きさを確認することができ、それらを適切に観察することができる。 (Drying of sample S)
In the imaging device 1 before the cover is attached, as explained in FIG. In this case, a clear image cannot be obtained from the image sensor 11. Therefore, for example, the sample S is dried without attaching the cover member 4. As a result, all microorganisms, including light microorganisms and microorganisms that do not sink easily, gather immediately above the image sensor 11 (within the depth of field x), so most microorganisms, etc. and their size, and can observe them appropriately.
　図１４に、試料Ｓを乾燥する前の画像の一例を示す。また、図１５に、試料Ｓを乾燥した後の画像の一例を示す。 FIG. 14 shows an example of an image of sample S before drying. Further, FIG. 15 shows an example of an image after drying the sample S.
　試料Ｓを乾燥する前は、図１４に示されるように、軽い微生物等や沈みにくい微生物等は、イメージセンサー１１の被写界深度ｘの範囲内に収まらず、ぼやけた状態で映っている。 Before the sample S is dried, as shown in FIG. 14, light microorganisms and microorganisms that do not sink easily are not within the depth of field x of the image sensor 11 and are imaged in a blurred state.
　一方、試料Ｓを乾燥した後は、図１５に示されるように、軽い微生物等や沈みにくい微生物等も含め、全ての微生物等は、イメージセンサー１１のすぐ上の位置にあるため、殆どの微生物等の形状や大きさを確認することができ、それらを適切に観察することができる。 On the other hand, after drying the sample S, as shown in FIG. You can confirm the shape and size of objects, etc., and observe them appropriately.
　（観察方法の例（その１））
　次に、図１６を参照して、試料Ｓの観察方法の例（その１）について説明する。 (Example of observation method (Part 1))
Next, with reference to FIG. 16, an example (part 1) of the observation method for the sample S will be described.
　ここでは、鮮明な画像を得られるようにするため、カバー部材４が使用される。 A cover member 4 is used here in order to obtain a clear image.
　最初に、試料Ｓを規定量測り取り（ステップＳ１１）、規定量の試料Ｓをイメージセンサー１１上の試料載置空間１Ａに滴下する（ステップＳ１２）。 First, a specified amount of the sample S is measured (step S11), and the specified amount of the sample S is dropped into the sample mounting space 1A above the image sensor 11 (step S12).
　次に、カバー部材４を撮像装置１に配置し、イメージセンサー１１上の試料載置空間１Ａに載置された試料Ｓの表面を平らにする（ステップＳ１３）。 Next, the cover member 4 is placed on the imaging device 1, and the surface of the sample S placed in the sample placement space 1A on the image sensor 11 is flattened (step S13).
　次に、照明５をカバー部材４の上の方に設置し、照明５の光がカバー部材４を透過して試料Ｓを照らすようにする（ステップＳ１４）。 Next, the illumination 5 is installed above the cover member 4 so that the light from the illumination 5 passes through the cover member 4 and illuminates the sample S (step S14).
　最後に、試料Ｓを撮影してイメージセンサー１１から出力される画像を情報処理装置２により取得し、取得した画像を表示装置に表示し、当該画像から試料Ｓに含まれる微生物等の観察を行う（ステップＳ１５）。 Finally, the sample S is photographed and the image output from the image sensor 11 is acquired by the information processing device 2, the acquired image is displayed on the display device, and microorganisms, etc. contained in the sample S are observed from the image. (Step S15).
　これにより、試料Ｓの含まれる全ての微生物等は、イメージセンサー１１の被写界深度ｘの範囲内に収まっているため、殆どの微生物等の形状や大きさを確認することができ、それらを適切に観察することができる。 As a result, all the microorganisms contained in the sample S are within the depth of field x of the image sensor 11, so it is possible to confirm the shape and size of most of the microorganisms, and Can be observed appropriately.
　（観察方法の例（その２））
　次に、図１７を参照して、試料Ｓの観察方法の例（その２）について説明する。 (Example of observation method (Part 2))
Next, with reference to FIG. 17, an example (part 2) of the method for observing the sample S will be described.
　ここでは、鮮明な画像を得られるようにするため、試料Ｓの乾燥が行われる。 Here, the sample S is dried in order to obtain a clear image.
　最初に、試料Ｓを規定量測り取り（ステップＳ２１）、規定量の試料Ｓをイメージセンサー１１上の試料載置空間１Ａに滴下する（ステップＳ２２）。 First, a specified amount of sample S is measured (step S21), and the specified amount of sample S is dropped into the sample placement space 1A above the image sensor 11 (step S22).
　次に、試料Ｓを乾燥させる（ステップＳ２３）。 Next, the sample S is dried (step S23).
　次に、照明５をカバー部材４の上の方に設置し、照明５の光が試料Ｓを照らすようにする（ステップＳ２４）。 Next, the illumination 5 is installed above the cover member 4 so that the light from the illumination 5 illuminates the sample S (step S24).
　最後に、試料Ｓを撮影してイメージセンサー１１から出力される画像を情報処理装置２により取得し、取得した画像を表示装置に表示し、当該画像から試料Ｓに含まれる微生物等の観察を行う（ステップＳ２５）。 Finally, the sample S is photographed and the image output from the image sensor 11 is acquired by the information processing device 2, the acquired image is displayed on the display device, and microorganisms, etc. contained in the sample S are observed from the image. (Step S25).
　これにより、試料Ｓの含まれる全ての微生物等は、イメージセンサー１１のすぐ上の位置に集まっている（被写界深度ｘの範囲内に入っている）ため、殆どの微生物等の形状や大きさを確認することができ、それらを適切に観察することができる。 As a result, all the microorganisms contained in the sample S are gathered at a position directly above the image sensor 11 (within the depth of field x), so the shape and size of most microorganisms, etc. be able to confirm the facts and observe them properly.
　以上詳述したように、実施形態によれば、イメージセンサーの上に載置される試料について明瞭な画像を得ることができる。 As detailed above, according to the embodiment, it is possible to obtain a clear image of the sample placed on the image sensor.
　本発明のいくつかの実施形態を説明したが、これらの実施形態は、例として提示したものであり、発明の範囲を限定することは意図していない。これら新規な実施形態は、その他の様々な形態で実施されることが可能であり、発明の要旨を逸脱しない範囲で、種々の省略、置き換え、変更を行うことができる。これら実施形態やその変形は、発明の範囲や要旨に含まれるとともに、特許請求の範囲に記載された発明とその均等の範囲に含まれる。 Although several embodiments of the present invention have been described, these embodiments are presented as examples and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, substitutions, and changes can be made without departing from the gist of the invention. These embodiments and their modifications are included within the scope and gist of the invention, as well as within the scope of the invention described in the claims and its equivalents.

Claims (10)

  1. 　撮像装置（１）に備えられるイメージセンサー（１１）の上の試料載置空間（１Ａ）に載置される液状の試料（Ｓ）の表面が平らになるように当該試料（Ｓ）の表面を押える押え部（４Ａ）を有する、イメージセンサーのカバー部材（４）。 The surface of the liquid sample (S) placed in the sample placement space (1A) above the image sensor (11) provided in the imaging device (1) is flattened. An image sensor cover member (4) having a presser foot (4A).
  2. 　前記押え部（４Ａ）は、前記試料（Ｓ）と接する平らな面を有する、
    　請求項１に記載の、イメージセンサーのカバー部材（４）。     The holding portion (4A) has a flat surface that contacts the sample (S);
    A cover (4) for an image sensor according to claim 1.
  3. 　前記カバー部材（４）のうち、少なくとも前記押え部（４Ａ）およびその鉛直方向にある部分は、光を透過する材質で形成されている、
    　請求項１に記載の、イメージセンサーのカバー部材（４）。     Of the cover member (4), at least the presser portion (4A) and a portion thereof in the vertical direction are formed of a material that transmits light.
    A cover member (4) for an image sensor according to claim 1.
  4. 　前記押え部（４Ａ）は、前記イメージセンサー（１１）の被写界深度の範囲内に前記試料（Ｓ）が収まるように当該試料（Ｓ）の表面を押える、
    　請求項１に記載の、イメージセンサーのカバー部材（４）。     The holding part (4A) presses the surface of the sample (S) so that the sample (S) falls within the depth of field of the image sensor (11).
    A cover member (4) for an image sensor according to claim 1.
  5. 　前記押え部（４Ａ）は、前記イメージセンサー（１１）の被写界深度の範囲内に前記試料（Ｓ）が収まるようにする鉛直方向長さを有する、
    　請求項１に記載の、イメージセンサーのカバー部材（４）。     The holding part (4A) has a vertical length that allows the sample (S) to fall within the depth of field of the image sensor (11).
    A cover member (4) for an image sensor according to claim 1.
  6. 　前記押え部（４Ａ）は、前記試料載置空間（１Ａ）の水平方向の断面形状および寸法に合わせた形状および寸法を有する、
    　請求項１に記載の、イメージセンサーのカバー部材（４）。     The holding part (4A) has a shape and dimensions that match the horizontal cross-sectional shape and dimensions of the sample placement space (1A).
    A cover member (4) for an image sensor according to claim 1.
  7. 　前記カバー部材（４）は、前記試料（Ｓ）が前記押え部（４Ａ）により押えられたときに余剰となる前記試料（Ｓ）の一部を外部に排出させる排水路（４Ｂ）を備えている、
    　請求項１に記載の、イメージセンサーのカバー部材（４）。     The cover member (4) is provided with a drainage channel (4B) for discharging a part of the sample (S) that becomes surplus when the sample (S) is pressed by the pressing portion (4A) to the outside.
    A cover (4) for an image sensor according to claim 1.
  8. 　請求項１乃至７のいずれか１項に記載の、イメージセンサーのカバー部材（４）と、
    　前記カバー部材（４）が適用される前記撮像装置（１）と
    　を具備する、観察システム。     An image sensor cover member (4) according to any one of claims 1 to 7;
    An observation system comprising: the imaging device (1) to which the cover member (4) is applied.
  9. 　撮像装置（１）に備えられるイメージセンサー（１１）の上の試料載置空間（１Ａ）に、液状の試料（Ｓ）を滴下する工程（Ｓ１２）と、
    　前記試料（Ｓ）の表面が平らになるように当該試料（Ｓ）の表面を押える押え部（４Ａ）を有するカバー部材（４）を前記撮像装置（１）に配置する工程（Ｓ１３）と、
    　前記撮像装置（１）により前記試料（Ｓ）を撮影して画像を取得する工程（Ｓ１５）と
    　を含む、観察方法。     A step (S12) of dropping a liquid sample (S) into the sample placement space (1A) above the image sensor (11) provided in the imaging device (1);
    a step (S13) of arranging a cover member (4) having a presser part (4A) for pressing the surface of the sample (S) so that the surface of the sample (S) is flat on the imaging device (1);
    An observation method comprising a step (S15) of photographing the sample (S) with the imaging device (1) to obtain an image.
  10. 　撮像装置（１）に備えられるイメージセンサー（１１）の上の試料載置空間（１Ａ）に、液状の試料（Ｓ）を滴下する工程（Ｓ２２）と、
    　前記試料（Ｓ）を乾燥させる工程（Ｓ２３）と、
    　前記撮像装置（１）により前記試料（Ｓ）を撮影して画像を取得する工程（Ｓ２５）と
    　を含む、観察方法。     A step (S22) of dropping a liquid sample (S) into the sample placement space (1A) above the image sensor (11) provided in the imaging device (1);
    a step (S23) of drying the sample (S);
    An observation method comprising a step (S25) of photographing the sample (S) with the imaging device (1) to obtain an image.
PCT/JP2023/003218 2022-09-22 2023-02-01 Image sensor cover member, observation system, and observation method WO2024062641A1 (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0943127A (en) * 1995-07-28 1997-02-14 Shimadzu Corp Compression tester for granular material
JP2010054426A (en) * 2008-08-29 2010-03-11 Toyobo Co Ltd Observation method of disease
JP2019500871A (en) * 2015-12-28 2019-01-17 キアジェン サイエンシズ，リミティド ライアビリティ Thin film flow cell
JP2020041928A (en) * 2018-09-11 2020-03-19 株式会社東芝 Self-check system
JP2021501322A (en) * 2017-10-26 2021-01-14 エッセンリックス コーポレーション High-speed measurement of platelets

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0943127A (en) * 1995-07-28 1997-02-14 Shimadzu Corp Compression tester for granular material
JP2010054426A (en) * 2008-08-29 2010-03-11 Toyobo Co Ltd Observation method of disease
JP2019500871A (en) * 2015-12-28 2019-01-17 キアジェン サイエンシズ，リミティド ライアビリティ Thin film flow cell
JP2021501322A (en) * 2017-10-26 2021-01-14 エッセンリックス コーポレーション High-speed measurement of platelets
JP2020041928A (en) * 2018-09-11 2020-03-19 株式会社東芝 Self-check system

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