WO2024050080A3 - Methods of biomanufacturing and purifying retinoids - Google Patents

Methods of biomanufacturing and purifying retinoids Download PDF

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Publication number
WO2024050080A3
WO2024050080A3 PCT/US2023/031838 US2023031838W WO2024050080A3 WO 2024050080 A3 WO2024050080 A3 WO 2024050080A3 US 2023031838 W US2023031838 W US 2023031838W WO 2024050080 A3 WO2024050080 A3 WO 2024050080A3
Authority
WO
WIPO (PCT)
Prior art keywords
retinoid
methods
isolating
crude oil
biomanufacturing
Prior art date
Application number
PCT/US2023/031838
Other languages
French (fr)
Other versions
WO2024050080A2 (en
Inventor
Binita BHATTACHARJEE
Thiane CARNEIRO
Annie PREECE-SCARINGE
Benjamin Yap
Original Assignee
Amyris Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc. filed Critical Amyris Inc.
Publication of WO2024050080A2 publication Critical patent/WO2024050080A2/en
Publication of WO2024050080A3 publication Critical patent/WO2024050080A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
    • C07C403/06—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by singly-bound oxygen atoms
    • C07C403/08—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by singly-bound oxygen atoms by hydroxy groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/16—Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are methods of isolating a retinoid that includes: providing a fermentation broth comprising the retinoid and an overlay; performing a liquid-solid and liquid-liquid separation on the fermentation broth to produce a crude oil and a waste heavy phase; mixing the crude oil with a solvent extraction system to extract the retinoid from the crude oil; and stripping the solvent extraction system from the extracted retinoid to thereby isolate the retinoid. Also provided herein are methods of isolating a retinoid from a composition including the retinoid and farnesol that include: providing a composition including the retinoid and farnesol; feeding the composition into a chromatography system; and collecting the retinoid, thereby isolating the retinoid.
PCT/US2023/031838 2022-09-01 2023-09-01 Methods of biomanufacturing and purifying retinoids WO2024050080A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263374274P 2022-09-01 2022-09-01
US63/374,274 2022-09-01

Publications (2)

Publication Number Publication Date
WO2024050080A2 WO2024050080A2 (en) 2024-03-07
WO2024050080A3 true WO2024050080A3 (en) 2024-04-18

Family

ID=88192119

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/031838 WO2024050080A2 (en) 2022-09-01 2023-09-01 Methods of biomanufacturing and purifying retinoids

Country Status (1)

Country Link
WO (1) WO2024050080A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160362709A1 (en) * 2011-07-29 2016-12-15 Industry-Academic Cooperation Foundation Gyeongsang National University Method for producing retinoid from microorganism
WO2022090549A1 (en) * 2020-10-30 2022-05-05 Dsm Ip Assets B.V. In situ two-phase extraction system

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160362709A1 (en) * 2011-07-29 2016-12-15 Industry-Academic Cooperation Foundation Gyeongsang National University Method for producing retinoid from microorganism
WO2022090549A1 (en) * 2020-10-30 2022-05-05 Dsm Ip Assets B.V. In situ two-phase extraction system

Also Published As

Publication number Publication date
WO2024050080A2 (en) 2024-03-07

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