WO2024033133A3 - Enzyme compositions comprising an amylase - Google Patents

Enzyme compositions comprising an amylase Download PDF

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Publication number
WO2024033133A3
WO2024033133A3 PCT/EP2023/071179 EP2023071179W WO2024033133A3 WO 2024033133 A3 WO2024033133 A3 WO 2024033133A3 EP 2023071179 W EP2023071179 W EP 2023071179W WO 2024033133 A3 WO2024033133 A3 WO 2024033133A3
Authority
WO
WIPO (PCT)
Prior art keywords
amylase
enzyme compositions
compositions
present
enzymes
Prior art date
Application number
PCT/EP2023/071179
Other languages
French (fr)
Other versions
WO2024033133A2 (en
Inventor
Oliver Spangenberg
Sonja KUEBELBECK
Claudia Esper
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of WO2024033133A2 publication Critical patent/WO2024033133A2/en
Publication of WO2024033133A3 publication Critical patent/WO2024033133A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C12N9/2494—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01078—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase

Abstract

In the present invention new enzyme compositions are provided. More specifically, the present invention provides enzyme compositions comprising an amylase and two or more additional enzymes selected from the group consisting of protease, mannanase, and cellulase and methods of making and using these enzymes compositions.
PCT/EP2023/071179 2022-08-11 2023-07-31 Enzyme compositions comprising an amylase WO2024033133A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP22189958.6 2022-08-11
EP22189958 2022-08-11

Publications (2)

Publication Number Publication Date
WO2024033133A2 WO2024033133A2 (en) 2024-02-15
WO2024033133A3 true WO2024033133A3 (en) 2024-03-21

Family

ID=82899227

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2023/071179 WO2024033133A2 (en) 2022-08-11 2023-07-31 Enzyme compositions comprising an amylase

Country Status (1)

Country Link
WO (1) WO2024033133A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008153805A2 (en) * 2007-05-30 2008-12-18 Danisco Us, Inc., Genencor Division Improved variants of the bacillus licheniformis alpha-amylase
WO2009061379A2 (en) * 2007-11-05 2009-05-14 Danisco Us Inc., Genencor Division Alpha-amylase variants with altered properties
WO2016079110A2 (en) * 2014-11-19 2016-05-26 Novozymes A/S Use of enzyme for cleaning
EP4047088A1 (en) * 2021-02-22 2022-08-24 Basf Se Amylase variants

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
US4933287A (en) 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
ATE110768T1 (en) 1986-08-29 1994-09-15 Novo Nordisk As ENZYMATIC DETERGENT ADDITIVE.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
US5389536A (en) 1986-11-19 1995-02-14 Genencor, Inc. Lipase from Pseudomonas mendocina having cutinase activity
WO1988009367A1 (en) 1987-05-29 1988-12-01 Genencor, Inc. Cutinase cleaning composition
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
JP3079276B2 (en) 1988-02-28 2000-08-21 天野製薬株式会社 Recombinant DNA, Pseudomonas sp. Containing the same, and method for producing lipase using the same
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
WO1990009446A1 (en) 1989-02-17 1990-08-23 Plant Genetic Systems N.V. Cutinase
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
DK0528828T4 (en) 1990-04-14 1998-08-31 Genencor Internat Gmbh Alkaline bacillus lipases, encoding DNA sequences, and bacilli producing such lipases
ATE169671T1 (en) 1990-09-13 1998-08-15 Novo Nordisk As LIPASE VARIANTS
US5869438A (en) 1990-09-13 1999-02-09 Novo Nordisk A/S Lipase variants
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DK88892D0 (en) 1992-07-06 1992-07-06 Novo Nordisk As CONNECTION
AU6267694A (en) 1993-02-26 1994-09-14 Procter & Gamble Company, The High active enzyme granulates
DK0652946T3 (en) 1993-04-27 2005-05-30 Genencor Int New lipase variants for use in detergents
JP2859520B2 (en) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ Lipase, microorganism producing the same, method for producing lipase, and detergent composition containing lipase
JPH07143883A (en) 1993-11-24 1995-06-06 Showa Denko Kk Lipase gene and mutant lipase
DE4343591A1 (en) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Process for the evolutionary design and synthesis of functional polymers based on shape elements and shape codes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
WO1995022615A1 (en) 1994-02-22 1995-08-24 Novo Nordisk A/S A method of preparing a variant of a lipolytic enzyme
AU2524695A (en) 1994-05-04 1995-11-29 Genencor International, Inc. Lipases with improved surfactant resistance
AU2884595A (en) 1994-06-20 1996-01-15 Unilever Plc Modified pseudomonas lipases and their use
AU2884695A (en) 1994-06-23 1996-01-19 Unilever Plc Modified pseudomonas lipases and their use
BE1008998A3 (en) 1994-10-14 1996-10-01 Solvay Lipase, microorganism producing the preparation process for the lipase and uses thereof.
KR970707275A (en) 1994-10-26 1997-12-01 안네 제케르 An enzyme having lipolytic activity (AN ENZYME WITH LIPOLYTIC ACTIVITY)
JPH08228778A (en) 1995-02-27 1996-09-10 Showa Denko Kk New lipase gene and production of lipase using the same
DE69633825T2 (en) 1995-07-14 2005-11-10 Novozymes A/S Modified enzyme with lipolytic activity
ES2221934T3 (en) 1995-08-11 2005-01-16 Novozymes A/S NEW LIPOLITIC ENZYMES.
DE19619221A1 (en) 1996-05-13 1997-11-20 Solvay Enzymes Gmbh & Co Kg Enzyme granules for washing and cleaning applications
JP4615723B2 (en) 1998-12-04 2011-01-19 ノボザイムス アクティーゼルスカブ Cutinase mutant
DK1149160T3 (en) 1999-01-25 2006-08-14 Novozymes As Recovery of a protein at elevated pH
EP1171581A1 (en) 1999-03-31 2002-01-16 Novozymes A/S Lipase variant
CN1426463A (en) 2000-06-02 2003-06-25 诺维信公司 Cutinase variants
EP1421187B1 (en) 2001-07-27 2007-10-10 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Systems for in vivo site-directed mutagenesis using oligonucleotides
EP2371949B1 (en) 2006-01-23 2017-04-19 Novozymes A/S Lipase variants
WO2008110498A1 (en) 2007-03-15 2008-09-18 Novozymes A/S Solubilization of protease crystals in fermentation broth
DE602007013545D1 (en) 2007-07-02 2011-05-12 Procter & Gamble Multi-chamber bag containing detergent
ES2603979T3 (en) 2008-02-29 2017-03-02 Novozymes A/S Polypeptides with hepatic activity and polynucleotides encoding them
EP2367923A2 (en) 2008-12-01 2011-09-28 Danisco US Inc. Enzymes with lipase activity
EP2408805A2 (en) 2009-03-18 2012-01-25 Danisco US Inc. Fungal cutinase from magnaporthe grisea
WO2011084417A1 (en) 2009-12-21 2011-07-14 Danisco Us Inc. Detergent compositions containing geobacillus stearothermophilus lipase and methods of use thereof
MX2012007168A (en) 2009-12-21 2012-07-23 Danisco Us Inc Detergent compositions containing thermobifida fusca lipase and methods of use thereof.
CN102712878A (en) 2009-12-21 2012-10-03 丹尼斯科美国公司 Detergent compositions containing bacillus subtilis lipase and methods of use thereof
WO2011150157A2 (en) 2010-05-28 2011-12-01 Danisco Us Inc. Detergent compositions containing streptomyces griseus lipase and methods of use thereof
EP2694537A1 (en) 2011-04-08 2014-02-12 Danisco US Inc. Compositions
EP2890775A1 (en) 2012-08-31 2015-07-08 The Procter & Gamble Company Laundry detergents and cleaning compositions comprising carboxyl group-containing polymers
US20160160197A1 (en) 2013-07-19 2016-06-09 Danisco Us Inc. Compositions and Methods Comprising a Lipolytic Enzyme Variant
EP3386612A1 (en) 2015-12-09 2018-10-17 Basf Se Method of purifying a protein from fermentation solids under desorbing conditions

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008153805A2 (en) * 2007-05-30 2008-12-18 Danisco Us, Inc., Genencor Division Improved variants of the bacillus licheniformis alpha-amylase
WO2009061379A2 (en) * 2007-11-05 2009-05-14 Danisco Us Inc., Genencor Division Alpha-amylase variants with altered properties
WO2016079110A2 (en) * 2014-11-19 2016-05-26 Novozymes A/S Use of enzyme for cleaning
EP4047088A1 (en) * 2021-02-22 2022-08-24 Basf Se Amylase variants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 25 April 2018 (2018-04-25), "SubName: Full=Alpha-amylase {ECO:0000313|EMBL:PLS03298.1};", XP002808519, retrieved from EBI accession no. UNIPROT:A0A2N5HCS2 Database accession no. A0A2N5HCS2 *

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