WO2024031046A3 - Protéines de fusion d' il-18 et procédés de production d'il-18 - Google Patents
Protéines de fusion d' il-18 et procédés de production d'il-18 Download PDFInfo
- Publication number
- WO2024031046A3 WO2024031046A3 PCT/US2023/071663 US2023071663W WO2024031046A3 WO 2024031046 A3 WO2024031046 A3 WO 2024031046A3 US 2023071663 W US2023071663 W US 2023071663W WO 2024031046 A3 WO2024031046 A3 WO 2024031046A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- fusion proteins
- proteins
- producing
- methods
- translocating
- Prior art date
Links
- 102000037865 fusion proteins Human genes 0.000 title abstract 3
- 108020001507 fusion proteins Proteins 0.000 title abstract 3
- 102000003810 Interleukin-18 Human genes 0.000 abstract 6
- 108090000171 Interleukin-18 Proteins 0.000 abstract 6
- 210000002472 endoplasmic reticulum Anatomy 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- -1 Fc region Proteins 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 102000004127 Cytokines Human genes 0.000 abstract 1
- 108090000695 Cytokines Proteins 0.000 abstract 1
- 102000007562 Serum Albumin Human genes 0.000 abstract 1
- 108010071390 Serum Albumin Proteins 0.000 abstract 1
- 108010003723 Single-Domain Antibodies Proteins 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 102000034238 globular proteins Human genes 0.000 abstract 1
- 108091005896 globular proteins Proteins 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne des protéines de fusion, qui sont composées d'éléments tels qu'une ou plusieurs protéines/un ou plusieurs polypeptides capables de translocation dans un réticulum endoplasmique (ER) et d'une interleukine 18 (IL-18) ou d'un fragment ou variant d'IL-18. Les protéines/polypeptides capables de translocation dans le ER comprennent, sans s'y limiter, une protéine immunoglobulaire telle qu'une région Fc, un anticorps VHH et un fragment scFv, et une protéine globulaire telle que la sérum albumine. L'IL-18 peut être un précurseur d'IL-18 qui ressemble à la pro-cytokine naturelle dans laquelle l'IL-18 est fusionnée à l'extrémité C-terminale de son propeptide ou d'un variant de propeptide. Dans certains modes de réalisation, les protéines de fusion peuvent également comprendre un lieur peptidique clivable.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263395476P | 2022-08-05 | 2022-08-05 | |
| US63/395,476 | 2022-08-05 | ||
| US202363463505P | 2023-05-02 | 2023-05-02 | |
| US63/463,505 | 2023-05-02 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2024031046A2 WO2024031046A2 (fr) | 2024-02-08 |
| WO2024031046A3 true WO2024031046A3 (fr) | 2024-05-10 |
Family
ID=89849927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2023/071663 WO2024031046A2 (fr) | 2022-08-05 | 2023-08-04 | Protéines de fusion d' il-18 et procédés de production d'il-18 |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2024031046A2 (fr) |
-
2023
- 2023-08-04 WO PCT/US2023/071663 patent/WO2024031046A2/fr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2024031046A2 (fr) | 2024-02-08 |
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