WO2023282367A1 - Cosmetic composition comprising cynanchum atratum extract - Google Patents

Cosmetic composition comprising cynanchum atratum extract Download PDF

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WO2023282367A1
WO2023282367A1 PCT/KR2021/008632 KR2021008632W WO2023282367A1 WO 2023282367 A1 WO2023282367 A1 WO 2023282367A1 KR 2021008632 W KR2021008632 W KR 2021008632W WO 2023282367 A1 WO2023282367 A1 WO 2023282367A1
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cosmetic composition
extract
white rice
flower
hollyhock
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PCT/KR2021/008632
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French (fr)
Korean (ko)
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박진수
변승연
김힐
이성은
주영아
김호열
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주식회사래디안
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Priority to PCT/KR2021/008632 priority Critical patent/WO2023282367A1/en
Publication of WO2023282367A1 publication Critical patent/WO2023282367A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Definitions

  • the present invention relates to a cosmetic composition having skin irritation relief and anti-inflammatory effects.
  • the skin has a primary function to protect our body from the external environment and a cosmetic function.
  • the skin is a body part directly exposed to the external environment, and when exposed to excessive ultraviolet rays or pollutants, skin irritation and inflammatory reactions such as erythema, edema, and itching are induced. Skin troubles caused by these stressors are aesthetic problems.
  • substances produced in the course of an inflammatory reaction incidentally cause pigmentation of the skin and accelerate the collapse of skin elastic fibers to affect the increase in skin wrinkles.
  • Cosmetics are used for the purpose of aesthetic function to clean or express the skin beautifully and defense function to protect the skin from harmful environment.
  • ingredients that are unavoidable but different from the purpose of skin protection such as surfactants, preservatives, fragrances, sunscreens, pigments, and functional Raw materials, etc., cause various side effects on the skin.
  • ingredients such as AHA (alpha hydroxy acid), retinol, and arbutin contained in functional products for the purpose of whitening, wrinkle improvement, and exfoliation are acting as the main cause of skin trouble.
  • inflammation is a mechanism to regenerate damaged parts changed by stimulation in the body, and the continuous inflammatory reaction promotes mucosal damage, resulting in pain, swelling, redness, heat, etc., resulting in functional disorders.
  • Macrophages are known to be involved in maintaining homeostasis through various host reactions.
  • nitric oxide and cyclooxygenase produced by inducible nitric oxide synthase Inflammation-promoting factors such as prostaglandin E2, which is produced in excess by )-2, are produced.
  • cytokines such as tumor necrosis factor- ⁇ , interleukin-6 and interleukin-1 ⁇ , which are inflammatory mediators in cells induced by lipopolysaccharide (LPS) to mediate various inflammatory responses.
  • LPS lipopolysaccharide
  • Patent Document 1 Korean Patent Registration No. 10-1127156
  • Patent Document 2 Korea Patent Registration No. 10-1397160
  • Patent Document 3 Korean Patent Registration No. 10-1768870
  • Patent Document 4 Korean Patent Registration No. 10-1309172
  • the present invention is to provide a cosmetic composition having skin irritation relief and anti-inflammatory effects from natural plants.
  • the cosmetic composition having skin irritation relief and anti-inflammatory effects according to the present invention preferably includes an extract of white rice flower, and more preferably further includes an extract of hollyhock flower.
  • the ratio of white rice flower extract: hollyhock flower extract is preferably 7 to 9.5: 3 to 0.5.
  • the anti-inflammatory effect of the cosmetic composition is preferably effective in the inflammation-mediated pathway expressing at least one selected from TNF- ⁇ , IL (Interleukin) -8 and IL (Interleukin) -1 ⁇ .
  • the cosmetic composition may further include at least one solvent selected from butylene glycol, hexanediol, ethylhexylglycerin, and phenoxyethanol.
  • the cosmetic composition may be a formulation selected from the group consisting of mask pack essence, softening lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, gel, lotion, ointment, patch, and spray.
  • the cosmetic composition according to the present invention contains extracts of white rice flower and hollyhock flower and has excellent skin and scalp irritation relieving effects, and also has anti-inflammatory effects, and is excellent in economic feasibility due to imported substitution.
  • 3 to 5 schematically show the results of RT-PCR in which inflammatory mediators were altered in the extracts according to the present invention.
  • the cosmetic composition having skin irritation relief and anti-inflammatory effects according to the present invention preferably includes an extract of white rice flower, and more preferably further includes an extract of hollyhock flower.
  • a step when a step is said to be located “on” or “before” another step, this is not only the case where a step is in a direct time-series relationship with another step, but also the mixing step after each step As such, the order of the two steps may include the same rights as in the case of an indirect time-series relationship in which the time-series order may change.
  • hollyhock extract which was inferior to white rice flower extract but had excellent anti-inflammatory effect, was selected as a candidate substance.
  • Hollyhock (scientific name: Althaea Rosea) is a perennial plant of the Malvaceae family.
  • the stem is about 2 meters high, and the leaves are alternate, heart-shaped, split into 5-7 pieces on the edge, and have sawtooth.
  • the fruit is a flat circle, and the carpel turns like a wheel and sticks to it, and ripens in September.
  • the root is called Chokgyugeun ( ⁇ ), and the flower is called Chokgyuhwa.
  • the cosmetic composition having skin irritation relief and anti-inflammatory effects according to the present invention preferably contains an extract of white rice flower and a hollyhock flower extract.
  • nitric oxide induces an inflammatory response.
  • LPS lipopolysaccharide
  • mouse macrophage cell line (Raw 264.7) was inoculated in a 24-well plate at a concentration of 1 ⁇ 10 5 cells/ml and cultured for 24 hours in an incubator at 37° C. and 5% CO 2 (see “Contol” in FIGS. 1 and 2). marked with ”).
  • serum-free media including DMEM, 10% FBS, 1% Ab, 2.5% HEPES, and 100 ng/ml LPS
  • L-NMMA NG-methyl-L-arginine acetate salt
  • the culture medium was collected and centrifuged at 4° C. and 12,000 rpm for 10 minutes, and only the supernatant was recovered.
  • FIGS. 1 and 2 The experimental results are schematically shown in FIGS. 1 and 2 .
  • the anti-inflammatory effect of the mixed extract of white rice flower and hollyhock flower was confirmed, but the extract (5:5) obtained by extracting the same amount of white rice flower and hollyhock flower was less effective than the single extract of white rice flower, and the ratio of the hollyhock flower extract was When it was relatively lower than the extract, it was confirmed that the anti-inflammatory effect was superior to that of the white rice flower alone extract.
  • the keratinocytes were inoculated in a 60-mm dish in the number of 2*10 6 and cultured for 24 hours (indicated as “Control” in FIGS. 3 to 5).
  • PBS phosphate buffer saline
  • a stimulus Surfactant_SLS
  • Dex dexamethasone
  • various compositions of this After further treatment with the composition of the invention, it was cultured for 24 to 48 hours.
  • RNA was isolated using TriZol, cDNA was synthesized, and expression levels of inflammatory mediators such as TNF- ⁇ , IL(Interleukin)-8, and IL(Interleukin)-1 ⁇ were confirmed.
  • the anti-inflammatory effect of the mixed extracts of white rice flower and hollyhock flower was confirmed, but the ratio of white rice flower: hollyhock flower was 8:2, the ratio of white rice flower: hollyhock flower was 9:1, and the ratio of white rice flower: hollyhock flower was 9.5:0.5. It was confirmed that the ratio of the extract further reduced the expression of the anti-inflammatory factor TNF- ⁇ than the white rice flower alone extract.
  • white rice flower hollyhock extract at a ratio of 7:3
  • white rice flower hollyhock flower at a ratio of 8:2
  • white rice flower hollyhock flower at a ratio of 9:1
  • the anti-inflammatory factor IL- 8 expression was further reduced.
  • the ratio of white rice: hollyhock is 7:3, the ratio of white rice: hollyhock is 8:2, the ratio of white rice: hollyhock is 9:1, and the ratio of white rice: hollyhock is 9.5:0.5. It was confirmed that the extract further reduced the expression of the anti-inflammatory factor IL-1 ⁇ than the white rice flower alone extract.
  • the ratio of white rice flower extract: hollyhock flower extract in the cosmetic composition is preferably 7-9.5: 3-0.5.
  • the anti-inflammatory effect of the cosmetic composition is preferably effective in the inflammation-mediated pathway expressing at least one selected from TNF- ⁇ , IL (Interleukin) -8 and IL (Interleukin) -1 ⁇ .
  • the cosmetic composition may further include at least one solvent selected from butylene glycol, hexanediol, ethylhexylglycerin, and phenoxyethanol.
  • the solvent is not limited thereto within the scope obvious to those skilled in the art. This solvent is necessary for the subsequent formation of the formulation and for the stability of the extract.
  • the cosmetic composition may be a formulation selected from the group consisting of mask pack essence, softening lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, gel, lotion, ointment, patch, and spray, but is not limited thereto.
  • composition of the present invention helps to relieve irritation, three adults aged 20 to 30 were tested.
  • Negative control group purified water
  • positive control group Dexpanthenol 2% solution
  • evaluation sample white rice flower ethanol extract; white rice flower: hollyhock flower ethanol mixture extract at a ratio of 9:1 ) After absorbing 200ul each of the two types, it was attached to 4 test sites for 10 minutes.
  • the present invention relates to a cosmetic composition having skin irritation relief and anti-inflammatory effects.

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Abstract

The objective of the present invention is to provide a cosmetic composition, derived from a natural plant, having skin irritation alleviating and anti-inflammatory effects. Since there are no Korean domestic farms growing Cyanchum atratum, and most Cyanchum atratum are imported and then commercialized, the economic feasibility thereof is not excellent. Accordingly, the objective of the present invention is to provide a substitute material that will improve economic feasibility while significantly enhancing the efficacy of a Cyanchum atratum extract.

Description

백미추출물을 포함하는 화장료 조성물Cosmetic composition containing white rice extract
본 발명은 피부 자극 완화 및 항염 효능을 갖는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition having skin irritation relief and anti-inflammatory effects.
피부는 외부환경으로부터 우리 몸을 보호하는 일차적인 기능과 미용적인 기능을 가지고 있다. The skin has a primary function to protect our body from the external environment and a cosmetic function.
구체적으로, 피부는 외부 환경에 직접적으로 노출되는 신체 부위로서, 과도한 자외선이나 오염물질 등에 노출되면 홍반 부종, 가려움 등의 피부 자극 및 염증 반응이 유발되는데, 이러한 스트레스원에 의한 피부 트러블은 미관상 문제가 될 뿐만 아니라, 염증 반응 과정에서 생성되는 물질들이 부수적으로 피부의 색소 침착을 일으키고, 피부 탄력 섬유의 붕괴를 촉진시켜 피부 주름의 증가에까지 영향을 미치는 것으로 알려져 있다.Specifically, the skin is a body part directly exposed to the external environment, and when exposed to excessive ultraviolet rays or pollutants, skin irritation and inflammatory reactions such as erythema, edema, and itching are induced. Skin troubles caused by these stressors are aesthetic problems. In addition, it is known that substances produced in the course of an inflammatory reaction incidentally cause pigmentation of the skin and accelerate the collapse of skin elastic fibers to affect the increase in skin wrinkles.
이러한 피부를 청결하게 하거나 아름답게 표현하는 미적 기능 및 유해 환경으로부터 피부를 보호하는 방어기능을 그 목적으로 하여 화장품을 사용한다.Cosmetics are used for the purpose of aesthetic function to clean or express the skin beautifully and defense function to protect the skin from harmful environment.
그러나, 피부를 보호하고 미화 청결을 위한 목적으로 다양하게 사용되는 피부 외용 제품들에 있어서, 사용이 불가피 하지만 피부 보호 목적과는 상이한 성분들, 예컨대 계면 활성제, 방부제, 향료, 자외선 차단제, 색소, 기능성 원료 등은 피부에 여러 가지 부작용을 발생시키고 있다. 특히 미백, 주름 개선, 각질제거 등을 목적으로 하는 기능성 제품에 함유되는 AHA(알파하이드록시산), 레티놀, 알부틴 등의 성분들은 피부 트러블을 일으키는 주된 원인으로 작용하고 있다.However, in skin external products that are variously used for the purpose of protecting the skin and beautifying and cleaning, ingredients that are unavoidable but different from the purpose of skin protection, such as surfactants, preservatives, fragrances, sunscreens, pigments, and functional Raw materials, etc., cause various side effects on the skin. In particular, ingredients such as AHA (alpha hydroxy acid), retinol, and arbutin contained in functional products for the purpose of whitening, wrinkle improvement, and exfoliation are acting as the main cause of skin trouble.
한편, 염증은 체내의 자극에 의해 변화된 손상 부위를 재생하려는 기전인데, 지속적인 염증 반응은 점막 손상을 촉진시켜 결과적으로 통증, 부종, 발적, 발열 등을 일으켜 기능 장애를 유발한다. 대식세포는 다양한 숙주반응을 통한 항상성 유지에 관여하는 것으로 알려져 있는데, 염증반응 시에는 유도성 산화질소 합성효소 (inducible nitric oxide synthase)에 의해 만들어지는 산화질소(nitric oxide)와 사이클로옥시제나아제 (cyclooxygenase)-2에 의해서 과량 만들어지는 프로스타글란딘(prostaglandin) E2 등과 같은 염증 촉진 인자들이 생성된다. 또한, 리포폴리사카라이드(lipopolysaccharide: LPS)에 의해 유발된 세포에서 염증매개 물질인 튜머 네크로시스 팩터(tumor necrosis factor)-α, 인터루킨(interleukin)-6 및 인터루킨(interleukin)-1β와 같은 사이토카인을 생산하여 다양한 염증반응을 매개한다.On the other hand, inflammation is a mechanism to regenerate damaged parts changed by stimulation in the body, and the continuous inflammatory reaction promotes mucosal damage, resulting in pain, swelling, redness, heat, etc., resulting in functional disorders. Macrophages are known to be involved in maintaining homeostasis through various host reactions. During inflammatory reactions, nitric oxide and cyclooxygenase produced by inducible nitric oxide synthase Inflammation-promoting factors such as prostaglandin E2, which is produced in excess by )-2, are produced. In addition, cytokines such as tumor necrosis factor-α, interleukin-6 and interleukin-1β, which are inflammatory mediators in cells induced by lipopolysaccharide (LPS) to mediate various inflammatory responses.
상기와 같은 이유로 피부 자극 및 염증의 생성을 줄일 수 있는 방법에 관한 연구가 활발히 진행되고 있으며, 항염제제로 사용되는 물질로 비스테로이드 계통의 벤지다민, 인도메타신, 이부프로펜 등이 있으며, 스테로이드 계통의 덱사메타손, 하이드로코티존 등이 있다. (Leung Y.F. et al., Mol Vis. Sep 5;9:425-39 (2003), Rocksen D. et al., Clin Exp Immunol. 122(2):249-56 (2000), Endo Y. et al., Res Commun Chem Pathol Pharmacol. 33(2):195-206 (1981), FAVOUR C.B. et al., Med Bull. 6(1):15-8 (1958), Sato S. et al., J Osaka Dent Univ.1(1):37-49 (1967))For the above reasons, research on methods for reducing skin irritation and inflammation is being actively conducted, and non-steroidal benzidamine, indomethacin, ibuprofen, etc. are used as anti-inflammatory substances, and steroidal dexamethasone. , hydrocortisone, etc. (Leung Y.F. et al., Mol Vis. Sep 5;9:425-39 (2003), Rocksen D. et al., Clin Exp Immunol. 122(2):249-56 (2000), Endo Y. et al. ., Res Commun Chem Pathol Pharmacol.33(2):195-206 (1981), FAVOUR C.B. et al., Med Bull.6(1):15-8 (1958), Sato S. et al., J Osaka Dent Univ.1(1):37-49 (1967))
그런데, 상기의 물질들은 화장품 원료로 사용할 수 없거나 사용량이 제한된 경우가 대부분이며, 특히 스테로이드 계통의 경우 피부 위축, 불면, 불안 등의 부작용을 동반하고 있다.However, most of the above substances cannot be used as raw materials for cosmetics or their usage is limited, and in particular, steroids are accompanied by side effects such as skin atrophy, insomnia, and anxiety.
따라서, 피부에 안전성이 우수한 천연 식물로부터 피부 자극 완화 및 항염 효능을 갖는 물질을 발굴할 필요성이 증대되고 있다.Therefore, there is an increasing need to discover substances having skin irritation relief and anti-inflammatory effects from natural plants with excellent skin safety.
일례로, 아래 특허문헌들에는 화장료 조성물 중에 백미꽃 추출물이나 접시꽃 추출물을 다양한 성분의 하나로써 개별적으로 포함시키려는 시도가 있었으나, 본 발명과 같은 만족스러운 결과를 도출하지 못했다.For example, in the patent documents below, attempts have been made to individually include a white rice flower extract or a hollyhock extract as one of various ingredients in a cosmetic composition, but satisfactory results have not been obtained as in the present invention.
(특허문헌 1) 한국특허등록번호 제10-1127156호(Patent Document 1) Korean Patent Registration No. 10-1127156
(특허문헌 2) 한국특허등록번호 제10-1397160호(Patent Document 2) Korea Patent Registration No. 10-1397160
(특허문헌 3) 한국특허등록번호 제10-1768870호(Patent Document 3) Korean Patent Registration No. 10-1768870
(특허문헌 4) 한국특허등록번호 제10-1309172호(Patent Document 4) Korean Patent Registration No. 10-1309172
본 발명은 천연 식물로부터 피부 자극 완화 및 항염 효능을 갖는 화장료 조성물을 제공하는 것이다.The present invention is to provide a cosmetic composition having skin irritation relief and anti-inflammatory effects from natural plants.
한편, 백미꽃이란 식물은 국내 재배 농가가 없으며, 대부분 수입하여 제품화 하고 있으므로, 경제성이 우수하지 못하다. 이에 따라 백미꽃 추출물의 효능을 현저히 향상시키면서 경제성을 보완할 대체 소재를 제공하는 것이다.On the other hand, since there are no domestic farms for growing white rice plants, and most of them are imported and commercialized, the economic feasibility is not excellent. Accordingly, it is to provide an alternative material to complement the economic feasibility while significantly improving the efficacy of the white rice flower extract.
본 발명에 따른 피부 자극 완화 및 항염 효능을 갖는 화장료 조성물은 백미꽃 추출물을 포함하는 것이 바람직하며, 보다 바람직하게는 접시꽃 추출물을 추가로 포함한다.The cosmetic composition having skin irritation relief and anti-inflammatory effects according to the present invention preferably includes an extract of white rice flower, and more preferably further includes an extract of hollyhock flower.
상기 화장료 조성물에서 백미꽃 추출물 : 접시꽃 추출물의 비율은 7~9.5 : 3~0.5인 것이 바람직하다.In the cosmetic composition, the ratio of white rice flower extract: hollyhock flower extract is preferably 7 to 9.5: 3 to 0.5.
상기 화장료 조성물의 항염 효능은 TNF-α, IL(Interleukin)-8 및 IL(Interleukin)-1α로부터 선택된 1종 이상을 발현하는 염증 매개 경로에 효과가 있는 것이 바람직하다.The anti-inflammatory effect of the cosmetic composition is preferably effective in the inflammation-mediated pathway expressing at least one selected from TNF-α, IL (Interleukin) -8 and IL (Interleukin) -1α.
상기 화장료 조성물은 부틸렌글리콜, 헥산디올, 에틸헥실글리세린 및 페녹시에탄올로부터 선택된 1종 이상의 용매를 추가로 포함할 수 있다.The cosmetic composition may further include at least one solvent selected from butylene glycol, hexanediol, ethylhexylglycerin, and phenoxyethanol.
상기 화장료 조성물은 마스크팩 에센스, 유연화장수, 영양화장수, 영양크림, 맛사지크림, 에센스, 팩, 젤, 로션, 연고, 패치 및 분무제로 이루어지는 군으로부터 선택되는 제형일 수 있다.The cosmetic composition may be a formulation selected from the group consisting of mask pack essence, softening lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, gel, lotion, ointment, patch, and spray.
본 발명에 따른 화장료 조성물은 백미꽃 및 접시꽃 추출물을 포함하여 피부 및 두피 자극 완화 효과가 우수하며, 또한 항염 효능을 갖는 효과가 있고, 수입 대체로 인해 경제성이 우수하다.The cosmetic composition according to the present invention contains extracts of white rice flower and hollyhock flower and has excellent skin and scalp irritation relieving effects, and also has anti-inflammatory effects, and is excellent in economic feasibility due to imported substitution.
도 1 및 도 2는 본 발명에 따른 추출물들의 NO-assay의 결과를 개략적으로 도시한 것이다.1 and 2 schematically show the results of NO-assay of extracts according to the present invention.
도 3 내지 도 5는 본 발명에 따른 추출물들을 염증 매개 인자를 변경한 RT-PCR의 결과를 개략적으로 도시한 것이다.3 to 5 schematically show the results of RT-PCR in which inflammatory mediators were altered in the extracts according to the present invention.
도 6 내지 도 8은 본 발명에 따른 추출물들의 자극 완화 실험 결과를 촬영한 사진이다.6 to 8 are photographs taken of the experimental results of stimulation relief of the extracts according to the present invention.
본 발명에 따른 피부 자극 완화 및 항염 효능을 갖는 화장료 조성물은 백미꽃 추출물을 포함하는 것이 바람직하며, 보다 바람직하게는 접시꽃 추출물을 추가로 포함한다.The cosmetic composition having skin irritation relief and anti-inflammatory effects according to the present invention preferably includes an extract of white rice flower, and more preferably further includes an extract of hollyhock flower.
이하, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본 발명의 실시형태를 들어 상세히 설명한다. 본 발명의 실시형태는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 더욱 완전하게 설명하기 위해서 제공되는 것이다. 따라서, 본 발명의 실시형태는 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 이하 설명하는 실시형태로 한정되는 것은 아니다.Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art. Therefore, the embodiments of the present invention can be modified in many different forms, and the scope of the present invention is not limited to the embodiments described below.
본 발명의 명세서 전체에서, 어떤 부분이 어떤 구성요소를 “포함”한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성요소를 더 포함하는 것을 의미한다.Throughout the specification of the present invention, when a part "includes" a certain component, it means that it further includes other components, not excluding other components unless otherwise stated.
본 발명의 명세서 전체에서, 어떤 단계가 다른 단계와 “상에” 또는 “전에” 위치하고 있다고 할 때, 이는 어떤 단계가 다른 단계와 직접적 시계열적인 관계에 있는 경우뿐만 아니라, 각 단계 후의 혼합하는 단계와 같이 두 단계의 순서에 시계열적 순서가 바뀔 수 있는 간접적 시계열적 관계에 있는 경우와 동일한 권리를 포함할 수 있다.Throughout the specification of the present invention, when a step is said to be located “on” or “before” another step, this is not only the case where a step is in a direct time-series relationship with another step, but also the mixing step after each step As such, the order of the two steps may include the same rights as in the case of an indirect time-series relationship in which the time-series order may change.
본 발명의 명세서 전체에서 사용되는 정도의 용어 “약”, “실질적으로” 등은 언급된 의미에 고유한 제조 및 물질 허용 오차가 제시될 때 그 수치에서 또는 그 수치에 근접한 의미로 사용되고, 본 발명의 이해를 돕기 위해 정확하거나 절대적인 수치가 언급된 개시 내용을 비양심적인 침해자가 부당하게 이용하는 것을 방지하기 위해 사용된다. 본원 명세서 전체에서 사용되는 용어 “~(하는) 단계” 또는 “~의 단계”는 “~를 위한 단계”를 의미하지 않는다.The terms "about," "substantially," and the like used throughout the specification of the present invention are used at or close to that value when manufacturing and material tolerances inherent in the stated meaning are given, and the present invention Accurate or absolute figures are used to prevent unfair use by unscrupulous infringers of the disclosed disclosures mentioned for the sake of understanding. The term “step of (doing)” or “step of” used throughout the present specification does not mean “step for”.
화장료 조성물에 사용될 수 있는 천연 추출물은 다양한 영양소 및 화합물을 포함하고 있으므로 그 작용이 복합적이다.Since natural extracts that can be used in cosmetic compositions contain various nutrients and compounds, their actions are complex.
다양한 천연 추출물 중에서, NO 생성 저해능으로 항염 효능을 스크리닝 하여, 백미꽃추출물보다는 부족하지만 항염 효능이 우수한 접시꽃추출물을 후보물질로 선정하였다.Among various natural extracts, anti-inflammatory efficacy was screened for NO production inhibition, and hollyhock extract, which was inferior to white rice flower extract but had excellent anti-inflammatory effect, was selected as a candidate substance.
접시꽃(학명 : Althaea Rosea)은 아욱과의 여러해살이풀. 줄기는 높이가 2미터 정도이며, 잎은 어긋나고 심장 모양으로 가장자리에 5~7개로 갈라지고 톱니가 있다. Hollyhock (scientific name: Althaea Rosea) is a perennial plant of the Malvaceae family. The stem is about 2 meters high, and the leaves are alternate, heart-shaped, split into 5-7 pieces on the edge, and have sawtooth.
열매는 편평한 원형으로 심피가 수레바퀴처럼 돌려 붙으며 9월에 익는다. 뿌리를 촉규근(蜀葵根)이라 하고, 꽃을 촉규화라고 하며, 점액이 있어 한방에서 점활제(粘滑劑)로 사용되고 있다.The fruit is a flat circle, and the carpel turns like a wheel and sticks to it, and ripens in September. The root is called Chokgyugeun (蜀葵根), and the flower is called Chokgyuhwa.
본 발명에 따른 피부 자극 완화 및 항염 효능을 갖는 화장료 조성물은 백미꽃 추출물 및 접시꽃 추출물을 포함하는 것이 바람직하다.The cosmetic composition having skin irritation relief and anti-inflammatory effects according to the present invention preferably contains an extract of white rice flower and a hollyhock flower extract.
따라서, 본 발명에 따른 다양한 조성의 조성물의 효능 비교를 위해 아래와 같이 실험을 수행하였다.Therefore, in order to compare the efficacy of the compositions of various compositions according to the present invention, experiments were performed as follows.
(실험예 1) 항염효능평가_NO assay(Experimental Example 1) Anti-inflammatory efficacy evaluation_NO assay
일산화질소 (NO)의 과도한 생성은 염증 반응을 유도하며, 이에 대한 효능 측정을 위하여, 세포를 LPS (lipopolysaccharide)로 자극하여 NO의 생성을 유도한 후, 시료에 의한 NO 생성 저해 효과를 Griess reagent 법으로 측정하였다.Excessive production of nitric oxide (NO) induces an inflammatory response. To measure its efficacy, cells were stimulated with LPS (lipopolysaccharide) to induce NO production, and then the NO production inhibitory effect by the sample was measured using the Griess reagent method. was measured.
구체적으로, 마우스 대식세포주(Raw 264.7)를 24-well 플레이트에 1×105 cells/ml의 농도로 접종하고 37℃, 5% CO2 인큐베이터에서 24시간 배양하였다(도 1 및 도 2에서 “Contol”로 표기함).Specifically, the mouse macrophage cell line (Raw 264.7) was inoculated in a 24-well plate at a concentration of 1×10 5 cells/ml and cultured for 24 hours in an incubator at 37° C. and 5% CO 2 (see “Contol” in FIGS. 1 and 2). marked with ”).
이어서, 혈청이 포함되지 않은 배지(DMEM, 10% FBS, 1% Ab, 2.5% HEPES, 100ng/ml LPS 포함)만를 이용하거나, 양성대조군인 L-NMMA (NG-methyl-L-arginine acetate salt) 50uM을 포함하거나, 다양한 조성의 본 발명의 조성물을 추가로 포함하는 배지를 500㎕씩 세포에 처리하여, 최종농도 10~200μg/ml의 농도가 되도록 하였다(도 1 및 도 2 참조). Subsequently, only serum-free media (including DMEM, 10% FBS, 1% Ab, 2.5% HEPES, and 100 ng/ml LPS) were used, or L-NMMA (NG-methyl-L-arginine acetate salt) as a positive control was used. Cells were treated with 500 μl of a medium containing 50 uM or additionally containing the composition of the present invention of various compositions, so that the final concentration was 10 to 200 μg/ml (see FIGS. 1 and 2).
24시간 배양한 후, 배지를 회수하여 4℃, 12,000rpm에서 10분간 원심분리를 시킨 후, 상층액만 회수하였다.After culturing for 24 hours, the culture medium was collected and centrifuged at 4° C. and 12,000 rpm for 10 minutes, and only the supernatant was recovered.
상층액 50ul만 취하여 96-well 플레이트에 넣고 동량의 Griess reagent(Sigma사, G4410)와 10분 동안 반응시킨 후, ELISA reader를 이용하여 540nm에서 흡광값을 측정하였다.Only 50ul of the supernatant was placed in a 96-well plate and reacted with the same amount of Griess reagent (Sigma, G4410) for 10 minutes, and then the absorbance value was measured at 540nm using an ELISA reader.
상기 실험 결과를 도 1 및 도 2에 개략적으로 도시하였다.The experimental results are schematically shown in FIGS. 1 and 2 .
도 1에 도시된 바와 같이, 백미꽃과 접시꽃의 혼합추출물은 모두 항염 효능이 확인되지만, 백미꽃과 접시꽃을 동량 추출한 추출물(5:5)은 백미꽃 단독 추출물보다 효능은 부족하였고, 접시꽃 추출물의 비율이 백미꽃 추출물보다 상대적으로 낮으면, 백미꽃 단독 추출물 보다 항염 효능이 우수함을 확인하였다.As shown in FIG. 1, the anti-inflammatory effect of the mixed extract of white rice flower and hollyhock flower was confirmed, but the extract (5:5) obtained by extracting the same amount of white rice flower and hollyhock flower was less effective than the single extract of white rice flower, and the ratio of the hollyhock flower extract was When it was relatively lower than the extract, it was confirmed that the anti-inflammatory effect was superior to that of the white rice flower alone extract.
추가로, 도 2에 도시된 바와 같이, 백미꽃:접시꽃이 7:3인 경우, 백미꽃:접시꽃이 8:2인 경우, 백미꽃:접시꽃이 9:1인 경우 및 백미꽃:접시꽃이 9.5:0.5인 경우의 추출물은 백미꽃 단독 추출물보다 항염 효능이 우수함을 확인하였다.In addition, as shown in FIG. 2, when the ratio of white rice flower:hollipop is 7:3, when the ratio of white rice flower:hollowock flower is 8:2, when the ratio of white rice flower:hollyhock flower is 9:1, and when the ratio of white rice flower:hollowock flower is 9.5:0.5 It was confirmed that the extract of was superior to the single extract of white rice flower in anti-inflammatory effect.
(실험예 2) RT-PCR(자극원: 계면활성제 SLS(Sodium Lauryl Sulfate))(Experimental Example 2) RT-PCR (stimulus source: surfactant SLS (Sodium Lauryl Sulfate))
각질형성세포주를 60-㎜ 접시에 2*106의 개수로 접종한 후, 24시간 동안 배양하였다(도 3 내지 도 5에서 “Control”로 표기함). 배양된 세포주를 PBS(phosphate buffer saline)를 이용하여 성장인자들을 제거한 후, 상기 세포가 배양된 배지에 자극원(계면활성제_SLS)만을 이용하거나, 양성대조군(Dex: dexamethasone) 및 다양한 조성의 본 발명의 조성물을 추가로 처리한 후, 24~48시간 동안 배양하였다. 배지를 제거한 후 TriZol을 이용하여 RNA를 분리한 후 cDNA를 합성한 후, TNF-α, IL(Interleukin)-8 및 IL(Interleukin)-1α의 염증매개인자의 발현 수준을 확인하였다.The keratinocytes were inoculated in a 60-mm dish in the number of 2*10 6 and cultured for 24 hours (indicated as “Control” in FIGS. 3 to 5). After removing growth factors from the cultured cell line using PBS (phosphate buffer saline), only a stimulus (surfactant_SLS) is used in the medium in which the cells are cultured, or a positive control group (Dex: dexamethasone) and various compositions of this After further treatment with the composition of the invention, it was cultured for 24 to 48 hours. After removing the medium, RNA was isolated using TriZol, cDNA was synthesized, and expression levels of inflammatory mediators such as TNF-α, IL(Interleukin)-8, and IL(Interleukin)-1α were confirmed.
상기 실험 결과를 도 3 내지 도 5에 개략적으로 도시하였다. The experimental results are schematically shown in FIGS. 3 to 5 .
도 3에 도시된 바와 같이, 백미꽃과 접시꽃의 혼합추출물은 모두 항염효능이 확인되지만, 백미꽃:접시꽃이 8:2인 비율, 백미꽃:접시꽃이 9:1인 비율, 백미꽃:접시꽃이 9.5:0.5인 비율의 추출물은 백미꽃 단독 추출물보다 항염인자 TNF-α 발현을 더욱 감소시킴을 확인하였다.As shown in FIG. 3, the anti-inflammatory effect of the mixed extracts of white rice flower and hollyhock flower was confirmed, but the ratio of white rice flower: hollyhock flower was 8:2, the ratio of white rice flower: hollyhock flower was 9:1, and the ratio of white rice flower: hollyhock flower was 9.5:0.5. It was confirmed that the ratio of the extract further reduced the expression of the anti-inflammatory factor TNF-α than the white rice flower alone extract.
또한, 도 4에 도시된 바와 같이, 백미꽃:접시꽃이 7:3인 비율, 백미꽃:접시꽃이 8:2인 비율, 백미꽃:접시꽃이 9:1인 비율의 추출물은 백미꽃 단독 추출물보다 항염인자 IL-8 발현을 더욱 감소시킴을 확인하였다.In addition, as shown in FIG. 4, white rice flower: hollyhock extract at a ratio of 7:3, white rice flower: hollyhock flower at a ratio of 8:2, and white rice flower: hollyhock flower at a ratio of 9:1, the anti-inflammatory factor IL- 8 expression was further reduced.
나아가, 도 5에 도시된 바와 같이, 백미꽃:접시꽃이 7:3인 비율, 백미꽃:접시꽃이 8:2인 비율, 백미꽃:접시꽃이 9:1인 비율, 백미꽃:접시꽃이 9.5:0.5인 비율의 추출물은 백미꽃 단독 추출물보다 항염인자 IL-1α 발현을 더욱 감소시킴을 확인하였다.Furthermore, as shown in FIG. 5, the ratio of white rice: hollyhock is 7:3, the ratio of white rice: hollyhock is 8:2, the ratio of white rice: hollyhock is 9:1, and the ratio of white rice: hollyhock is 9.5:0.5. It was confirmed that the extract further reduced the expression of the anti-inflammatory factor IL-1α than the white rice flower alone extract.
상기 실험 결과로부터 상기 화장료 조성물에서 백미꽃 추출물 : 접시꽃 추출물의 비율은 7~9.5 : 3~0.5인 것이 바람직하다.From the experimental results, the ratio of white rice flower extract: hollyhock flower extract in the cosmetic composition is preferably 7-9.5: 3-0.5.
또한, 상기 화장료 조성물의 항염 효능은 TNF-α, IL(Interleukin)-8 및 IL(Interleukin)-1α로부터 선택된 1종 이상을 발현하는 염증 매개 경로에 효과가 있는 것이 바람직하다.In addition, the anti-inflammatory effect of the cosmetic composition is preferably effective in the inflammation-mediated pathway expressing at least one selected from TNF-α, IL (Interleukin) -8 and IL (Interleukin) -1α.
상기 화장료 조성물은 부틸렌글리콜, 헥산디올, 에틸헥실글리세린 및 페녹시에탄올로부터 선택된 1종 이상의 용매를 추가로 포함할 수 있다. 상기 용매는 당업자에게 자명한 범위에서 이에 제한되지 않는다. 상기 용매는 후속하는 제형의 형성 및 추출물의 안정성을 위해 필요하다.The cosmetic composition may further include at least one solvent selected from butylene glycol, hexanediol, ethylhexylglycerin, and phenoxyethanol. The solvent is not limited thereto within the scope obvious to those skilled in the art. This solvent is necessary for the subsequent formation of the formulation and for the stability of the extract.
상기 화장료 조성물은 마스크팩 에센스, 유연화장수, 영양화장수, 영양크림, 맛사지크림, 에센스, 팩, 젤, 로션, 연고, 패치 및 분무제로 이루어지는 군으로부터 선택되는 제형일 수 있으나, 이에 제한되는 것이 아니다.The cosmetic composition may be a formulation selected from the group consisting of mask pack essence, softening lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, gel, lotion, ointment, patch, and spray, but is not limited thereto.
이하에서는 본 발명에 따른 화장료 조성물의 자극완화 정도를 아래와 같이 실험하였다.Hereinafter, the degree of irritation relief of the cosmetic composition according to the present invention was tested as follows.
(실시예 3) 자극완화실험(Example 3) irritation relief test
본 발명의 조성물이 자극완화에 도움을 주는지 확인하기 위해 20~30세 성인 3명을 대상으로 시험하였다.In order to confirm whether the composition of the present invention helps to relieve irritation, three adults aged 20 to 30 were tested.
1) 대일밴드 중형(19x72mm, 거즈 면적 13x25mm)의 안쪽 거즈에 자극원(Phenoxyethanol 2% solution)을 200㎕ 흡수시킨 뒤, 팔꿈치 아래 시험 부위 4군데에 1시간 부착하였다.1) After absorbing 200 μl of the irritant (Phenoxyethanol 2% solution) on the inner gauze of a large band (19x72mm, gauze area 13x25mm), it was attached to four test sites below the elbow for 1 hour.
2) 1시간 경과 후 자극원 처리 밴드를 제거하고, 남은 수분은 닦아주었다.2) After 1 hour, the stimulant treatment band was removed, and the remaining moisture was wiped off.
3) 홍반 유발이 확인되면 사진촬영 진행 후 자극 위치 및 시료 처리 위치를 표시하였다.3) When erythema induction was confirmed, the location of stimulation and sample processing were marked after taking pictures.
4) 자극원 처리 시와 동일한 밴드를 이용하여 안쪽 거즈에 음성대조군(정제수), 양성대조군(Dexpanthenol 2% solution) 및 평가시료(백미꽃 에탄올 추출물; 백미꽃:접시꽃이 9:1인 비율의 에탄올 혼합 추출물) 2종을 각각 200ul씩 흡수시킨 뒤, 시험부위 4군데에 10분 부착하였다.4) Negative control group (purified water), positive control group (Dexpanthenol 2% solution) and evaluation sample (white rice flower ethanol extract; white rice flower: hollyhock flower ethanol mixture extract at a ratio of 9:1 ) After absorbing 200ul each of the two types, it was attached to 4 test sites for 10 minutes.
5) 10분 경과 후 자극완화 시료 처리 밴드를 제거하고, 남은 수분을 닦아주었다.5) After 10 minutes, the irritation relief sample treatment band was removed and the remaining moisture was wiped off.
6) 자극완화 시료 처리 직후와 10분 경과 후 육안으로 홍반 완화 효능을 확인한 뒤 사진촬영을 진행하였다.6) Immediately after the irritation relief sample treatment and after 10 minutes, the erythema relief effect was confirmed with the naked eye, and then a photo was taken.
상기 실험결과를 도 6 내지 도 8에 나타내었다.The experimental results are shown in FIGS. 6 to 8.
결과적으로, 우수 자극완화 성분으로 알려진 Dexpanthenol의 홍반 완화능과 백미꽃 에탄올 단독추출물 및 백미꽃+접시꽃 혼합추출물의 홍반 완화능은 피험자 모두 유사한 것으로 확인되었다. As a result, it was confirmed that the erythema mitigating ability of Dexpanthenol, known as an excellent irritation relieving ingredient, and the erythema mitigating ability of the white rice flower ethanol extract and the white rice flower + hollyhock flower mixed extract were similar for all subjects.
특히, 도 6에서 보듯이, 피험자 1의 경우 백미꽃 에탄올 단독 추출물보다 백미꽃+접시꽃 혼합추출물에서 육안으로 보아도 확실한 홍반 완화가 확인되었다.In particular, as shown in FIG. 6, in the case of subject 1, clear erythema relief was confirmed even with the naked eye in the mixed extract of white rice flower + hollyhock flower than in the single extract of white rice flower ethanol.
이상에서 본 발명의 실시예에 대하여 상세하게 설명하였지만 본 발명의 권리범위는 이에 한정되는 것은 아니고, 청구범위에 기재된 본 발명의 기술적 사상을 벗어나지 않는 범위 내에서 다양한 수정 및 변형이 가능하다는 것은 당 기술분야의 통상의 지식을 가진 자에게는 자명할 것이다.Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and variations are possible without departing from the technical spirit of the present invention described in the claims. It will be obvious to those skilled in the art.
본 발명은 피부 자극 완화 및 항염 효능을 갖는 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition having skin irritation relief and anti-inflammatory effects.

Claims (6)

  1. 백미꽃 추출물을 포함하는 화장료 조성물로서, 피부 자극 완화 및 항염 효능을 갖는 것을 특징으로 하는 화장료 조성물.A cosmetic composition comprising a white rice flower extract, characterized in that it has skin irritation relief and anti-inflammatory effects.
  2. 제 1 항에 있어서, 접시꽃 추출물을 추가로 포함하는 것을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 1, further comprising a hollyhock extract.
  3. 제 2 항에 있어서, 상기 백미꽃 추출물 : 접시꽃 추출물의 비율이 7~9.5 : 3~0.5인 것을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 2, wherein the ratio of the white rice flower extract to the hollyhock flower extract is 7-9.5: 3-0.5.
  4. 제 1 항 또는 제 2 항에 있어서, 상기 항염 효능이 TNF-α, IL(Interleukin)-8 및 IL(Interleukin)-1α로부터 선택된 1종 이상을 발현하는 염증 매개 경로에 효과가 있는 것을 특징으로 하는 화장료 조성물.The method of claim 1 or 2, wherein the anti-inflammatory effect is characterized in that it has an effect on an inflammatory mediating pathway expressing at least one selected from TNF-α, IL (Interleukin)-8 and IL (Interleukin)-1α cosmetic composition.
  5. 제 1 항 또는 제 2 항에 있어서, 상기 화장료 조성물이 부틸렌글리콜, 헥산디올, 에틸헥실글리세린 및 페녹시에탄올로부터 선택된 1종 이상의 용매를 추가로 포함하는 것을 특징으로 하는 화장료 조성물.The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition further comprises at least one solvent selected from butylene glycol, hexanediol, ethylhexylglycerin and phenoxyethanol.
  6. 제 1 항 또는 제 2 항에 있어서, 상기 화장료 조성물이 마스크팩 에센스, 유연화장수, 영양화장수, 영양크림, 맛사지크림, 에센스, 팩, 젤, 로션, 연고, 패치 및 분무제로 이루어지는 군으로부터 선택되는 제형인 것을 특징으로 하는 화장료 조성물.The formulation of claim 1 or 2, wherein the cosmetic composition is selected from the group consisting of mask pack essence, softening lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, gel, lotion, ointment, patch and spray A cosmetic composition, characterized in that.
PCT/KR2021/008632 2021-07-07 2021-07-07 Cosmetic composition comprising cynanchum atratum extract WO2023282367A1 (en)

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