WO2023218241A1 - Heteroaryl derivative compounds, and pharmaceutical composition comprising thereof - Google Patents
Heteroaryl derivative compounds, and pharmaceutical composition comprising thereof Download PDFInfo
- Publication number
- WO2023218241A1 WO2023218241A1 PCT/IB2023/000256 IB2023000256W WO2023218241A1 WO 2023218241 A1 WO2023218241 A1 WO 2023218241A1 IB 2023000256 W IB2023000256 W IB 2023000256W WO 2023218241 A1 WO2023218241 A1 WO 2023218241A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- compound
- pharmaceutically acceptable
- acceptable salt
- optical isomer
- cancer
- Prior art date
Links
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 31
- 125000001072 heteroaryl group Chemical group 0.000 title claims abstract description 17
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims description 91
- 150000003839 salts Chemical class 0.000 claims description 64
- 102100033479 RAF proto-oncogene serine/threonine-protein kinase Human genes 0.000 claims description 49
- 201000010099 disease Diseases 0.000 claims description 37
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 37
- 230000003287 optical effect Effects 0.000 claims description 35
- 238000000034 method Methods 0.000 claims description 33
- 239000000126 substance Substances 0.000 claims description 26
- 206010028980 Neoplasm Diseases 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 18
- 201000011510 cancer Diseases 0.000 claims description 17
- 125000001475 halogen functional group Chemical group 0.000 claims description 17
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 12
- 125000000753 cycloalkyl group Chemical group 0.000 claims description 11
- 230000002265 prevention Effects 0.000 claims description 10
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims description 9
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims description 9
- 230000035772 mutation Effects 0.000 claims description 9
- 239000004480 active ingredient Substances 0.000 claims description 8
- 125000003118 aryl group Chemical group 0.000 claims description 8
- 239000012472 biological sample Substances 0.000 claims description 8
- 206010009944 Colon cancer Diseases 0.000 claims description 5
- 201000001441 melanoma Diseases 0.000 claims description 5
- 208000024770 Thyroid neoplasm Diseases 0.000 claims description 4
- 125000001559 cyclopropyl group Chemical group [H]C1([H])C([H])([H])C1([H])* 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 201000002510 thyroid cancer Diseases 0.000 claims description 4
- 208000001333 Colorectal Neoplasms Diseases 0.000 claims description 3
- 206010033128 Ovarian cancer Diseases 0.000 claims description 3
- 206010061535 Ovarian neoplasm Diseases 0.000 claims description 3
- 125000003709 fluoroalkyl group Chemical group 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 claims description 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 2
- -1 heteroaryl derivative compound Chemical class 0.000 abstract description 18
- 210000004027 cell Anatomy 0.000 description 25
- 239000003814 drug Substances 0.000 description 11
- 230000004663 cell proliferation Effects 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 102100024193 Mitogen-activated protein kinase 1 Human genes 0.000 description 7
- 125000000217 alkyl group Chemical group 0.000 description 7
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 7
- 150000002390 heteroarenes Chemical class 0.000 description 7
- 125000000592 heterocycloalkyl group Chemical group 0.000 description 7
- 102000016914 ras Proteins Human genes 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 102000004232 Mitogen-Activated Protein Kinase Kinases Human genes 0.000 description 6
- 108090000744 Mitogen-Activated Protein Kinase Kinases Proteins 0.000 description 6
- 230000004913 activation Effects 0.000 description 6
- 238000001994 activation Methods 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 229910052731 fluorine Inorganic materials 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 125000004432 carbon atom Chemical group C* 0.000 description 5
- 229910052801 chlorine Inorganic materials 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 125000005843 halogen group Chemical group 0.000 description 5
- 230000026731 phosphorylation Effects 0.000 description 5
- 238000006366 phosphorylation reaction Methods 0.000 description 5
- 230000019491 signal transduction Effects 0.000 description 5
- UJOBWOGCFQCDNV-UHFFFAOYSA-N 9H-carbazole Chemical compound C1=CC=C2C3=CC=CC=C3NC2=C1 UJOBWOGCFQCDNV-UHFFFAOYSA-N 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 4
- NQRYJNQNLNOLGT-UHFFFAOYSA-N Piperidine Chemical compound C1CCNCC1 NQRYJNQNLNOLGT-UHFFFAOYSA-N 0.000 description 4
- IOJUPLGTWVMSFF-UHFFFAOYSA-N benzothiazole Chemical compound C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 description 4
- 239000012091 fetal bovine serum Substances 0.000 description 4
- 238000004020 luminiscence type Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 125000001424 substituent group Chemical group 0.000 description 4
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 208000003174 Brain Neoplasms Diseases 0.000 description 3
- 239000004215 Carbon black (E152) Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101000771237 Homo sapiens Serine/threonine-protein kinase A-Raf Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 3
- 102100029437 Serine/threonine-protein kinase A-Raf Human genes 0.000 description 3
- 230000008512 biological response Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 3
- 125000001188 haloalkyl group Chemical group 0.000 description 3
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 150000002430 hydrocarbons Chemical class 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 3
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 3
- 230000003211 malignant effect Effects 0.000 description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 150000007522 mineralic acids Chemical class 0.000 description 3
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- XSCHRSMBECNVNS-UHFFFAOYSA-N quinoxaline Chemical compound N1=CC=NC2=CC=CC=C21 XSCHRSMBECNVNS-UHFFFAOYSA-N 0.000 description 3
- 229940124597 therapeutic agent Drugs 0.000 description 3
- FCEHBMOGCRZNNI-UHFFFAOYSA-N 1-benzothiophene Chemical compound C1=CC=C2SC=CC2=C1 FCEHBMOGCRZNNI-UHFFFAOYSA-N 0.000 description 2
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010005949 Bone cancer Diseases 0.000 description 2
- 208000018084 Bone neoplasm Diseases 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
- 102000009465 Growth Factor Receptors Human genes 0.000 description 2
- 108010009202 Growth Factor Receptors Proteins 0.000 description 2
- XKMLYUALXHKNFT-UUOKFMHZSA-N Guanosine-5'-triphosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XKMLYUALXHKNFT-UUOKFMHZSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 206010025323 Lymphomas Diseases 0.000 description 2
- YNAVUWVOSKDBBP-UHFFFAOYSA-N Morpholine Chemical compound C1COCCN1 YNAVUWVOSKDBBP-UHFFFAOYSA-N 0.000 description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 102000001253 Protein Kinase Human genes 0.000 description 2
- KYQCOXFCLRTKLS-UHFFFAOYSA-N Pyrazine Chemical compound C1=CN=CC=N1 KYQCOXFCLRTKLS-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 2
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 229910052783 alkali metal Inorganic materials 0.000 description 2
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 2
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 description 2
- 125000004429 atom Chemical group 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 125000002619 bicyclic group Chemical group 0.000 description 2
- 201000009036 biliary tract cancer Diseases 0.000 description 2
- 208000020790 biliary tract neoplasm Diseases 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 229910052794 bromium Inorganic materials 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 238000003570 cell viability assay Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- WDECIBYCCFPHNR-UHFFFAOYSA-N chrysene Chemical compound C1=CC=CC2=CC=C3C4=CC=CC=C4C=CC3=C21 WDECIBYCCFPHNR-UHFFFAOYSA-N 0.000 description 2
- 208000029742 colonic neoplasm Diseases 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 125000001995 cyclobutyl group Chemical group [H]C1([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 2
- TXCDCPKCNAJMEE-UHFFFAOYSA-N dibenzofuran Chemical compound C1=CC=C2C3=CC=CC=C3OC2=C1 TXCDCPKCNAJMEE-UHFFFAOYSA-N 0.000 description 2
- IYYZUPMFVPLQIF-UHFFFAOYSA-N dibenzothiophene Chemical compound C1=CC=C2C3=CC=CC=C3SC2=C1 IYYZUPMFVPLQIF-UHFFFAOYSA-N 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 125000003784 fluoroethyl group Chemical group [H]C([H])(F)C([H])([H])* 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 206010017758 gastric cancer Diseases 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 201000005787 hematologic cancer Diseases 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 2
- AWJUIBRHMBBTKR-UHFFFAOYSA-N isoquinoline Chemical compound C1=NC=CC2=CC=CC=C21 AWJUIBRHMBBTKR-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000001394 metastastic effect Effects 0.000 description 2
- 206010061289 metastatic neoplasm Diseases 0.000 description 2
- WCPAKWJPBJAGKN-UHFFFAOYSA-N oxadiazole Chemical compound C1=CON=N1 WCPAKWJPBJAGKN-UHFFFAOYSA-N 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 239000006187 pill Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 108060006633 protein kinase Proteins 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- BBEAQIROQSPTKN-UHFFFAOYSA-N pyrene Chemical compound C1=CC=C2C=CC3=CC=CC4=CC=C1C2=C43 BBEAQIROQSPTKN-UHFFFAOYSA-N 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 206010038038 rectal cancer Diseases 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- GGCZERPQGJTIQP-UHFFFAOYSA-N sodium;9,10-dioxoanthracene-2-sulfonic acid Chemical compound [Na+].C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 GGCZERPQGJTIQP-UHFFFAOYSA-N 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- JNYWVERKQKRXSL-PHDIDXHHSA-N (1r,4r)-2-oxa-5-azabicyclo[2.2.2]octane Chemical compound C1C[C@]2([H])CN[C@@]1([H])CO2 JNYWVERKQKRXSL-PHDIDXHHSA-N 0.000 description 1
- CJQNJRRDTPULTL-KNVOCYPGSA-N (1r,5s)-3-azabicyclo[3.2.1]octane Chemical compound C1[C@@]2([H])CC[C@]1([H])CNC2 CJQNJRRDTPULTL-KNVOCYPGSA-N 0.000 description 1
- QBYIENPQHBMVBV-HFEGYEGKSA-N (2R)-2-hydroxy-2-phenylacetic acid Chemical compound O[C@@H](C(O)=O)c1ccccc1.O[C@@H](C(O)=O)c1ccccc1 QBYIENPQHBMVBV-HFEGYEGKSA-N 0.000 description 1
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- JYEUMXHLPRZUAT-UHFFFAOYSA-N 1,2,3-triazine Chemical compound C1=CN=NN=C1 JYEUMXHLPRZUAT-UHFFFAOYSA-N 0.000 description 1
- YJTKZCDBKVTVBY-UHFFFAOYSA-N 1,3-Diphenylbenzene Chemical compound C1=CC=CC=C1C1=CC=CC(C=2C=CC=CC=2)=C1 YJTKZCDBKVTVBY-UHFFFAOYSA-N 0.000 description 1
- BCMCBBGGLRIHSE-UHFFFAOYSA-N 1,3-benzoxazole Chemical compound C1=CC=C2OC=NC2=C1 BCMCBBGGLRIHSE-UHFFFAOYSA-N 0.000 description 1
- WNXJIVFYUVYPPR-UHFFFAOYSA-N 1,3-dioxolane Chemical compound C1COCO1 WNXJIVFYUVYPPR-UHFFFAOYSA-N 0.000 description 1
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- YHIIJNLSGULWAA-UHFFFAOYSA-N 1,4-thiazinane 1-oxide Chemical compound O=S1CCNCC1 YHIIJNLSGULWAA-UHFFFAOYSA-N 0.000 description 1
- RTBFRGCFXZNCOE-UHFFFAOYSA-N 1-methylsulfonylpiperidin-4-one Chemical compound CS(=O)(=O)N1CCC(=O)CC1 RTBFRGCFXZNCOE-UHFFFAOYSA-N 0.000 description 1
- WJFKNYWRSNBZNX-UHFFFAOYSA-N 10H-phenothiazine Chemical compound C1=CC=C2NC3=CC=CC=C3SC2=C1 WJFKNYWRSNBZNX-UHFFFAOYSA-N 0.000 description 1
- TZMSYXZUNZXBOL-UHFFFAOYSA-N 10H-phenoxazine Chemical compound C1=CC=C2NC3=CC=CC=C3OC2=C1 TZMSYXZUNZXBOL-UHFFFAOYSA-N 0.000 description 1
- MYKQKWIPLZEVOW-UHFFFAOYSA-N 11h-benzo[a]carbazole Chemical compound C1=CC2=CC=CC=C2C2=C1C1=CC=CC=C1N2 MYKQKWIPLZEVOW-UHFFFAOYSA-N 0.000 description 1
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 description 1
- ZKAMEFMDQNTDFK-UHFFFAOYSA-N 1h-imidazo[4,5-b]pyrazine Chemical compound C1=CN=C2NC=NC2=N1 ZKAMEFMDQNTDFK-UHFFFAOYSA-N 0.000 description 1
- AMFYRKOUWBAGHV-UHFFFAOYSA-N 1h-pyrazolo[4,3-b]pyridine Chemical compound C1=CN=C2C=NNC2=C1 AMFYRKOUWBAGHV-UHFFFAOYSA-N 0.000 description 1
- QPEJAHMNOVMSOZ-UHFFFAOYSA-N 2-azaspiro[3.3]heptane Chemical compound C1CCC21CNC2 QPEJAHMNOVMSOZ-UHFFFAOYSA-N 0.000 description 1
- MGADZUXDNSDTHW-UHFFFAOYSA-N 2H-pyran Chemical compound C1OC=CC=C1 MGADZUXDNSDTHW-UHFFFAOYSA-N 0.000 description 1
- ZPSJGADGUYYRKE-UHFFFAOYSA-N 2H-pyran-2-one Chemical compound O=C1C=CC=CO1 ZPSJGADGUYYRKE-UHFFFAOYSA-N 0.000 description 1
- JVQIKJMSUIMUDI-UHFFFAOYSA-N 3-pyrroline Chemical compound C1NCC=C1 JVQIKJMSUIMUDI-UHFFFAOYSA-N 0.000 description 1
- GAMYYCRTACQSBR-UHFFFAOYSA-N 4-azabenzimidazole Chemical compound C1=CC=C2NC=NC2=N1 GAMYYCRTACQSBR-UHFFFAOYSA-N 0.000 description 1
- BNNMDMGPZUOOOE-UHFFFAOYSA-N 4-methylbenzenesulfonic acid Chemical class CC1=CC=C(S(O)(=O)=O)C=C1.CC1=CC=C(S(O)(=O)=O)C=C1 BNNMDMGPZUOOOE-UHFFFAOYSA-N 0.000 description 1
- MJQSRSOTRPMVKB-UHFFFAOYSA-N 5h-imidazo[4,5-c]pyridazine Chemical compound C1=NNC2=NC=NC2=C1 MJQSRSOTRPMVKB-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 208000024893 Acute lymphoblastic leukemia Diseases 0.000 description 1
- 208000014697 Acute lymphocytic leukaemia Diseases 0.000 description 1
- 208000031261 Acute myeloid leukaemia Diseases 0.000 description 1
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 1
- 206010061424 Anal cancer Diseases 0.000 description 1
- 208000007860 Anus Neoplasms Diseases 0.000 description 1
- 206010003571 Astrocytoma Diseases 0.000 description 1
- 208000010839 B-cell chronic lymphocytic leukemia Diseases 0.000 description 1
- 208000032791 BCR-ABL1 positive chronic myelogenous leukemia Diseases 0.000 description 1
- 208000005440 Basal Cell Neoplasms Diseases 0.000 description 1
- 206010004146 Basal cell carcinoma Diseases 0.000 description 1
- KHNYNFUTFKJLDD-UHFFFAOYSA-N Benzo[j]fluoranthene Chemical compound C1=CC(C=2C3=CC=CC=C3C=CC=22)=C3C2=CC=CC3=C1 KHNYNFUTFKJLDD-UHFFFAOYSA-N 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical group N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 1
- 206010005003 Bladder cancer Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 208000010833 Chronic myeloid leukaemia Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- 206010061825 Duodenal neoplasm Diseases 0.000 description 1
- 206010014733 Endometrial cancer Diseases 0.000 description 1
- 206010014759 Endometrial neoplasm Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 1
- 208000022072 Gallbladder Neoplasms Diseases 0.000 description 1
- 208000031852 Gastrointestinal stromal cancer Diseases 0.000 description 1
- 208000032612 Glial tumor Diseases 0.000 description 1
- 206010018338 Glioma Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical compound Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 1
- WRYCSMQKUKOKBP-UHFFFAOYSA-N Imidazolidine Chemical compound C1CNCN1 WRYCSMQKUKOKBP-UHFFFAOYSA-N 0.000 description 1
- 208000007766 Kaposi sarcoma Diseases 0.000 description 1
- 208000008839 Kidney Neoplasms Diseases 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 206010023825 Laryngeal cancer Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 208000031422 Lymphocytic Chronic B-Cell Leukemia Diseases 0.000 description 1
- 208000004059 Male Breast Neoplasms Diseases 0.000 description 1
- 208000030070 Malignant epithelial tumor of ovary Diseases 0.000 description 1
- 208000032271 Malignant tumor of penis Diseases 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 208000005410 Mediastinal Neoplasms Diseases 0.000 description 1
- 208000003445 Mouth Neoplasms Diseases 0.000 description 1
- 208000034578 Multiple myelomas Diseases 0.000 description 1
- 101100523539 Mus musculus Raf1 gene Proteins 0.000 description 1
- 201000003793 Myelodysplastic syndrome Diseases 0.000 description 1
- 208000033761 Myelogenous Chronic BCR-ABL Positive Leukemia Diseases 0.000 description 1
- 208000033776 Myeloid Acute Leukemia Diseases 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 208000010191 Osteitis Deformans Diseases 0.000 description 1
- 208000007571 Ovarian Epithelial Carcinoma Diseases 0.000 description 1
- 206010061328 Ovarian epithelial cancer Diseases 0.000 description 1
- ZCQWOFVYLHDMMC-UHFFFAOYSA-N Oxazole Chemical compound C1=COC=N1 ZCQWOFVYLHDMMC-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000027868 Paget disease Diseases 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 208000000821 Parathyroid Neoplasms Diseases 0.000 description 1
- 206010061336 Pelvic neoplasm Diseases 0.000 description 1
- 208000002471 Penile Neoplasms Diseases 0.000 description 1
- 206010034299 Penile cancer Diseases 0.000 description 1
- 208000009565 Pharyngeal Neoplasms Diseases 0.000 description 1
- 206010034811 Pharyngeal cancer Diseases 0.000 description 1
- PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- 208000007913 Pituitary Neoplasms Diseases 0.000 description 1
- 201000005746 Pituitary adenoma Diseases 0.000 description 1
- 206010061538 Pituitary tumour benign Diseases 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 108700020978 Proto-Oncogene Proteins 0.000 description 1
- 102000052575 Proto-Oncogene Human genes 0.000 description 1
- WTKZEGDFNFYCGP-UHFFFAOYSA-N Pyrazole Chemical compound C=1C=NNC=1 WTKZEGDFNFYCGP-UHFFFAOYSA-N 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- IWYDHOAUDWTVEP-UHFFFAOYSA-N R-2-phenyl-2-hydroxyacetic acid Natural products OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 description 1
- 102000042888 RAF family Human genes 0.000 description 1
- 108091082327 RAF family Proteins 0.000 description 1
- 101710141955 RAF proto-oncogene serine/threonine-protein kinase Proteins 0.000 description 1
- 208000015634 Rectal Neoplasms Diseases 0.000 description 1
- 206010038389 Renal cancer Diseases 0.000 description 1
- 201000000582 Retinoblastoma Diseases 0.000 description 1
- 208000004337 Salivary Gland Neoplasms Diseases 0.000 description 1
- 206010061934 Salivary gland cancer Diseases 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 206010041067 Small cell lung cancer Diseases 0.000 description 1
- 206010054184 Small intestine carcinoma Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000032383 Soft tissue cancer Diseases 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 208000024313 Testicular Neoplasms Diseases 0.000 description 1
- 206010057644 Testis cancer Diseases 0.000 description 1
- FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical compound C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
- 208000000728 Thymus Neoplasms Diseases 0.000 description 1
- 206010062129 Tongue neoplasm Diseases 0.000 description 1
- 206010044002 Tonsil cancer Diseases 0.000 description 1
- 208000006842 Tonsillar Neoplasms Diseases 0.000 description 1
- SLGBZMMZGDRARJ-UHFFFAOYSA-N Triphenylene Natural products C1=CC=C2C3=CC=CC=C3C3=CC=CC=C3C2=C1 SLGBZMMZGDRARJ-UHFFFAOYSA-N 0.000 description 1
- 208000023915 Ureteral Neoplasms Diseases 0.000 description 1
- 206010046392 Ureteric cancer Diseases 0.000 description 1
- 206010046431 Urethral cancer Diseases 0.000 description 1
- 206010046458 Urethral neoplasms Diseases 0.000 description 1
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 206010047741 Vulval cancer Diseases 0.000 description 1
- 208000004354 Vulvar Neoplasms Diseases 0.000 description 1
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 208000004064 acoustic neuroma Diseases 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 125000002015 acyclic group Chemical group 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 201000005188 adrenal gland cancer Diseases 0.000 description 1
- 208000024447 adrenal gland neoplasm Diseases 0.000 description 1
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 1
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
- 150000008044 alkali metal hydroxides Chemical class 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 229910001860 alkaline earth metal hydroxide Inorganic materials 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 125000003277 amino group Chemical class 0.000 description 1
- 125000004103 aminoalkyl group Chemical group 0.000 description 1
- 201000000315 ampulla of Vater cancer Diseases 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- JFCQEDHGNNZCLN-UHFFFAOYSA-N anhydrous glutaric acid Natural products OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 201000011165 anus cancer Diseases 0.000 description 1
- 239000011260 aqueous acid Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 229960005261 aspartic acid Drugs 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- RFRXIWQYSOIBDI-UHFFFAOYSA-N benzarone Chemical compound CCC=1OC2=CC=CC=C2C=1C(=O)C1=CC=C(O)C=C1 RFRXIWQYSOIBDI-UHFFFAOYSA-N 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- OGEBRHQLRGFBNV-RZDIXWSQSA-N chembl2036808 Chemical compound C12=NC(NCCCC)=NC=C2C(C=2C=CC(F)=CC=2)=NN1C[C@H]1CC[C@H](N)CC1 OGEBRHQLRGFBNV-RZDIXWSQSA-N 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 208000032852 chronic lymphocytic leukemia Diseases 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 208000035250 cutaneous malignant susceptibility to 1 melanoma Diseases 0.000 description 1
- 208000030381 cutaneous melanoma Diseases 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 125000000582 cycloheptyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
- 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
- 125000000640 cyclooctyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical compound C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 201000000312 duodenum cancer Diseases 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 201000004101 esophageal cancer Diseases 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 208000024519 eye neoplasm Diseases 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- GVEPBJHOBDJJJI-UHFFFAOYSA-N fluoranthrene Natural products C1=CC(C2=CC=CC=C22)=C3C2=CC=CC3=C1 GVEPBJHOBDJJJI-UHFFFAOYSA-N 0.000 description 1
- RMBPEFMHABBEKP-UHFFFAOYSA-N fluorene Chemical compound C1=CC=C2C3=C[CH]C=CC3=CC2=C1 RMBPEFMHABBEKP-UHFFFAOYSA-N 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 235000011087 fumaric acid Nutrition 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 201000010175 gallbladder cancer Diseases 0.000 description 1
- 208000010749 gastric carcinoma Diseases 0.000 description 1
- 201000011587 gastric lymphoma Diseases 0.000 description 1
- 201000008822 gestational choriocarcinoma Diseases 0.000 description 1
- 208000005017 glioblastoma Diseases 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940097043 glucuronic acid Drugs 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 201000010235 heart cancer Diseases 0.000 description 1
- 208000024348 heart neoplasm Diseases 0.000 description 1
- 208000024200 hematopoietic and lymphoid system neoplasm Diseases 0.000 description 1
- 208000006359 hepatoblastoma Diseases 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- WJRBRSLFGCUECM-UHFFFAOYSA-N hydantoin Chemical compound O=C1CNC(=O)N1 WJRBRSLFGCUECM-UHFFFAOYSA-N 0.000 description 1
- 229940091173 hydantoin Drugs 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-M hydrogensulfate Chemical compound OS([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-M 0.000 description 1
- 229940071870 hydroiodic acid Drugs 0.000 description 1
- RCCPEORTSYDPMB-UHFFFAOYSA-N hydroxy benzenecarboximidothioate Chemical compound OSC(=N)C1=CC=CC=C1 RCCPEORTSYDPMB-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical class [H]O* 0.000 description 1
- 125000002768 hydroxyalkyl group Chemical group 0.000 description 1
- YAMHXTCMCPHKLN-UHFFFAOYSA-N imidazolidin-2-one Chemical compound O=C1NCCN1 YAMHXTCMCPHKLN-UHFFFAOYSA-N 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- ZLTPDFXIESTBQG-UHFFFAOYSA-N isothiazole Chemical compound C=1C=NSC=1 ZLTPDFXIESTBQG-UHFFFAOYSA-N 0.000 description 1
- CTAPFRYPJLPFDF-UHFFFAOYSA-N isoxazole Chemical compound C=1C=NOC=1 CTAPFRYPJLPFDF-UHFFFAOYSA-N 0.000 description 1
- 201000010982 kidney cancer Diseases 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 206010023841 laryngeal neoplasm Diseases 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 208000012987 lip and oral cavity carcinoma Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 201000005249 lung adenocarcinoma Diseases 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 201000005243 lung squamous cell carcinoma Diseases 0.000 description 1
- 201000003175 male breast cancer Diseases 0.000 description 1
- 208000010907 male breast carcinoma Diseases 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 208000016035 malignant germ cell tumor of ovary Diseases 0.000 description 1
- 208000006178 malignant mesothelioma Diseases 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 208000026045 malignant tumor of parathyroid gland Diseases 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 208000027202 mammary Paget disease Diseases 0.000 description 1
- IWYDHOAUDWTVEP-UHFFFAOYSA-M mandelate Chemical compound [O-]C(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-M 0.000 description 1
- 229960002510 mandelic acid Drugs 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 201000000349 mediastinal cancer Diseases 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 206010027191 meningioma Diseases 0.000 description 1
- LVWZTYCIRDMTEY-UHFFFAOYSA-N metamizole Chemical compound O=C1C(N(CS(O)(=O)=O)C)=C(C)N(C)N1C1=CC=CC=C1 LVWZTYCIRDMTEY-UHFFFAOYSA-N 0.000 description 1
- 208000037819 metastatic cancer Diseases 0.000 description 1
- 208000011575 metastatic malignant neoplasm Diseases 0.000 description 1
- 229940098779 methanesulfonic acid Drugs 0.000 description 1
- 150000004947 monocyclic arenes Chemical class 0.000 description 1
- 125000002950 monocyclic group Chemical group 0.000 description 1
- 201000005962 mycosis fungoides Diseases 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 208000002154 non-small cell lung carcinoma Diseases 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- NIHNNTQXNPWCJQ-UHFFFAOYSA-N o-biphenylenemethane Natural products C1=CC=C2CC3=CC=CC=C3C2=C1 NIHNNTQXNPWCJQ-UHFFFAOYSA-N 0.000 description 1
- 201000008106 ocular cancer Diseases 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 201000008042 ovarian germ cell cancer Diseases 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 201000002528 pancreatic cancer Diseases 0.000 description 1
- 208000008443 pancreatic carcinoma Diseases 0.000 description 1
- 201000009612 pediatric lymphoma Diseases 0.000 description 1
- 201000002628 peritoneum cancer Diseases 0.000 description 1
- 229950000688 phenothiazine Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- LFSXCDWNBUNEEM-UHFFFAOYSA-N phthalazine Chemical compound C1=NN=CC2=CC=CC=C21 LFSXCDWNBUNEEM-UHFFFAOYSA-N 0.000 description 1
- XKJCHHZQLQNZHY-UHFFFAOYSA-N phthalimide Chemical compound C1=CC=C2C(=O)NC(=O)C2=C1 XKJCHHZQLQNZHY-UHFFFAOYSA-N 0.000 description 1
- XUWHAWMETYGRKB-UHFFFAOYSA-N piperidin-2-one Chemical compound O=C1CCCCN1 XUWHAWMETYGRKB-UHFFFAOYSA-N 0.000 description 1
- 208000021310 pituitary gland adenoma Diseases 0.000 description 1
- 201000003437 pleural cancer Diseases 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 230000009822 protein phosphorylation Effects 0.000 description 1
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 1
- FQOBINBWTPHVEO-UHFFFAOYSA-N pyrazino[2,3-b]pyrazine Chemical compound N1=CC=NC2=NC=CN=C21 FQOBINBWTPHVEO-UHFFFAOYSA-N 0.000 description 1
- USPWKWBDZOARPV-UHFFFAOYSA-N pyrazolidine Chemical compound C1CNNC1 USPWKWBDZOARPV-UHFFFAOYSA-N 0.000 description 1
- PBMFSQRYOILNGV-UHFFFAOYSA-N pyridazine Chemical compound C1=CC=NN=C1 PBMFSQRYOILNGV-UHFFFAOYSA-N 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- YEYHFKBVNARCNE-UHFFFAOYSA-N pyrido[2,3-b]pyrazine Chemical compound N1=CC=NC2=CC=CN=C21 YEYHFKBVNARCNE-UHFFFAOYSA-N 0.000 description 1
- BWESROVQGZSBRX-UHFFFAOYSA-N pyrido[3,2-d]pyrimidine Chemical compound C1=NC=NC2=CC=CN=C21 BWESROVQGZSBRX-UHFFFAOYSA-N 0.000 description 1
- 125000004076 pyridyl group Chemical group 0.000 description 1
- HNJBEVLQSNELDL-UHFFFAOYSA-N pyrrolidin-2-one Chemical compound O=C1CCCN1 HNJBEVLQSNELDL-UHFFFAOYSA-N 0.000 description 1
- JWVCLYRUEFBMGU-UHFFFAOYSA-N quinazoline Chemical compound N1=CN=CC2=CC=CC=C21 JWVCLYRUEFBMGU-UHFFFAOYSA-N 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- SBYHFKPVCBCYGV-UHFFFAOYSA-N quinuclidine Chemical compound C1CC2CCN1CC2 SBYHFKPVCBCYGV-UHFFFAOYSA-N 0.000 description 1
- 108010014186 ras Proteins Proteins 0.000 description 1
- 102000027426 receptor tyrosine kinases Human genes 0.000 description 1
- 108091008598 receptor tyrosine kinases Proteins 0.000 description 1
- 208000020615 rectal carcinoma Diseases 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 201000009410 rhabdomyosarcoma Diseases 0.000 description 1
- 102200055464 rs113488022 Human genes 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229930195734 saturated hydrocarbon Chemical group 0.000 description 1
- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 208000037968 sinus cancer Diseases 0.000 description 1
- 201000000849 skin cancer Diseases 0.000 description 1
- 201000003708 skin melanoma Diseases 0.000 description 1
- 208000000587 small cell lung carcinoma Diseases 0.000 description 1
- 201000002314 small intestine cancer Diseases 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 201000011096 spinal cancer Diseases 0.000 description 1
- 208000014618 spinal cord cancer Diseases 0.000 description 1
- 125000003003 spiro group Chemical group 0.000 description 1
- 206010041823 squamous cell carcinoma Diseases 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 201000000498 stomach carcinoma Diseases 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 229940095064 tartrate Drugs 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 201000003120 testicular cancer Diseases 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- RAOIDOHSFRTOEL-UHFFFAOYSA-N tetrahydrothiophene Chemical compound C1CCSC1 RAOIDOHSFRTOEL-UHFFFAOYSA-N 0.000 description 1
- 150000003536 tetrazoles Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- VLLMWSRANPNYQX-UHFFFAOYSA-N thiadiazole Chemical compound C1=CSN=N1.C1=CSN=N1 VLLMWSRANPNYQX-UHFFFAOYSA-N 0.000 description 1
- VJYJJHQEVLEOFL-UHFFFAOYSA-N thieno[3,2-b]thiophene Chemical compound S1C=CC2=C1C=CS2 VJYJJHQEVLEOFL-UHFFFAOYSA-N 0.000 description 1
- BRNULMACUQOKMR-UHFFFAOYSA-N thiomorpholine Chemical compound C1CSCCN1 BRNULMACUQOKMR-UHFFFAOYSA-N 0.000 description 1
- 229930192474 thiophene Natural products 0.000 description 1
- IBBLKSWSCDAPIF-UHFFFAOYSA-N thiopyran Chemical compound S1C=CC=C=C1 IBBLKSWSCDAPIF-UHFFFAOYSA-N 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 201000009377 thymus cancer Diseases 0.000 description 1
- 201000006134 tongue cancer Diseases 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
- 125000005580 triphenylene group Chemical group 0.000 description 1
- XLRPYZSEQKXZAA-OCAPTIKFSA-N tropane Chemical compound C1CC[C@H]2CC[C@@H]1N2C XLRPYZSEQKXZAA-OCAPTIKFSA-N 0.000 description 1
- 229930004006 tropane Natural products 0.000 description 1
- 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 201000011294 ureter cancer Diseases 0.000 description 1
- 201000005112 urinary bladder cancer Diseases 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 208000037965 uterine sarcoma Diseases 0.000 description 1
- 206010046885 vaginal cancer Diseases 0.000 description 1
- 208000013139 vaginal neoplasm Diseases 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 description 1
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 description 1
- 201000005102 vulva cancer Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
- C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
- C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
- C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
Definitions
- the present invention relates to heteroaryl derivative compounds and a pharmaceutical composition comprising thereof. Specifically, the present invention relates to heteroaryl derivative compounds having RAF inhibitory activity.
- the RAS/RAF/MEK/ERK protein kinase signaling pathway plays a very important role in the regulation of cellular function, and is specifically involved in cell proliferation, differentiation, survival, and angiogenesis (Biology of the Cell, 2001, 93, 53-62).
- GTP guanosine triphosphate
- phosphorylation and activation of the RAF protein in the protoplasmic membrane proceeds.
- the activated RAF protein phosphorylates and activates the MEK protein
- the MEK protein phosphorylates and activates the ERK protein.
- Translocation of activated ERK from the cytoplasm to the nucleus results in regulation and phosphorylation of transcription factors such as Elk-1 and Myc.
- RAF protooncogenes are serine/thr protein kinases that transmit signals from growth factor receptors activated in the cell membrane to transcription factors in the nucleus.
- the activation of the RAF protein is accompanied by phosphorylation of tyrosine, serine, and threonine residues of RAF protein, and direct phosphorylation by receptor tyrosine kinase or phosphorylation by protein phosphorylation enzymes controlled by these receptors is known as the mechanism of RAF activation.
- RAS is involved in the activation of RAF. Signals reaching RAF are then transferred to the nucleus via a signaling pathway leading to the RAF/MEK/ERK protein kinase.
- RAF acts as a major propagator of RAS function, providing a theoretical background for chemotherapy in the case of cancer with mutations or activations of RAS in inhibiting the action of this protein.
- RAF proteins have three isoforms of ARAF, BRAF, and CRAF (also known as RAF-1) with three functions(Biochim. Biophys. Acta., 2003, 1653, 25- 40), all three RAF genes are expressed in most tissues, and high expression of BRAF occurs in neurocellular tissues and ARAF occurs in urinary reproductive tissues.
- each RAF family has a very similar amino acid sequence, biochemical activity and biological functionality are distinguished from each other (Exp. Cell. Res. 1999, 253, 34-46).
- BRAF is an important isoform protein related to cell proliferation and is an important target of oncogenic RAS.
- Abnormal mutations in the body have only been identified in BRAF cases, and are known to occur at a frequency of 30-60% in malignant skin melanoma (Nature, 2002, 417, 949-954), 30-50% in thyroid cancer, 5-20% in colon cancer, and 30% or less in ovarian cancer (Nature Rev. Mol. Cell Biology, 20045, 875 and 885). So far, more than 45 BRAF mutations have been known, but the most frequent mutation is that valine number 600 mutates with glutamic acid (V600E), which is observed in more than 90% of human cancers. This mutation is believed to increase the kinase activity of BRAF and transmit RAF/MEK/ERK signals to sub-signaling pathways that include structural activity of ERK as a result of RAS and growth factor receptor activation.
- the present inventors confirmed that the compounds of the present invention have an anticancer effect by inhibiting the activity of RAF, thereby completing the present invention.
- An object of the present invention is to provide a heteroaryl derivative having a novel structure, an stereoisomer thereof, or a pharmaceutically acceptable salt thereof.
- Another object of the present invention is to provide a method for preparing the heteroaryl derivative compounds.
- Still another object of the present invention is to provide a pharmaceutical use of the heteroaryl derivative compounds, and specifically, to a pharmaceutical composition for the treatment or prevention of RAF-related diseases comprising the heteroaryl derivative compounds as an active ingredient, use of the compounds for the treatment or prevention of RAF-related diseases, or a method for treating or preventing RAF-related diseases comprising administering the compounds.
- the present invention provides a compound represented by following Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof: (Chemical Formula 1) in the Chemical Formula 1, X is CH 2 , or O;
- Y is CH or N
- Ri is -Ci-ealkyl, -Ci-ehaloalkyl, aryl or heteroaryl in which at least one H of the aryl or heteroaryl ring may be substituted with -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
- R 2 and R3 are each independently -H, -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
- R4 is -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
- R5 is -Ci-ealkyl, -Ci-ehaloalkyl, cycloalkyl, or -Ci-ealkyl-cycloalkyl.
- the compound represented by Chemical Formula 1 or a pharmaceutically acceptable salt thereof may be selected from the group consisting of the compounds of Examples 1 to 15 described in Table 1.
- alkyl may refer to a straight or branched chain acyclic, cyclic, or saturated hydrocarbon to which they are bonded.
- “Ci-ealkyl” may indicate an alkyl containing 1 to 6 carbon atoms.
- acyclic alkyl may include, but is not limited to, methyl, ethyl, n-propyl, n-butyl, isopropyl, secbutyl, isobutyl, tert-butyl, or the like.
- Cyclic alkyl may be used interchangeably with “cycloalkyl” as used herein, and as an example, may include, but is not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, or the like.
- the cycloalkyl is monocyclic.
- the cycloalkyl is 3- 7-membered.
- halo or “halogen” may be F, Cl, Br, or I.
- haloalkyl may mean a straight or branched chain alkyl (hydrocarbon) having one or more halo-substituted carbon atoms as defined herein.
- examples of the haloalkyl may include, but are not limited to, methyl, ethyl, propyl, isopropyl, isobutyl or n-butyl independently substituted with one or more halogens, such as F, Cl, Br, or I.
- hydroxyalkyl may indicate a straight or branched chain alkyl (hydrocarbon) having a carbon atom substituted with -hydroxy (-OH).
- haloalkyl may include, but are not limited to, methyl, ethyl, propyl, isopropyl, isobutyl or n-butyl independently substituted with one or more -OH.
- aminoalkyl may mean a straight or branched chain alkyl (hydrocarbon) having a carbon atom substituted with amino (-NR'R").
- R' and R" may be each independently selected from the group consisting of hydrogen and Ci-ealkyl, and the selected R' and R" may be each independently substituted or unsubstituted.
- heterocycloalkyl may mean a ring containing 1 to 5 heteroatoms selected from N, O and S as atoms forming the ring, and may be saturated or partially unsaturated.
- heterocycloalkyl is not aromatic.
- when unsaturated it may be referred to as a heterocycloalkene.
- heterocycloalkyl may be a single ring or a multiple ring such as a spiro ring, a bridged ring or a fused ring.
- “3- to 12-membered heterocycloalkyl” may indicate a heterocycloalkyl containing 3 to 12 atoms forming a ring.
- the heterocycloalkyl may include, but is not limited to, pyrrolidine, piperidine, imidazolidine, pyrazolidine, butyrolactam, valerolactam, imidazolidinone, hydantoin, dioxolane, phthalimide, piperidine, pyrimidin-2,4(lH,3H)-dione, 1,4-di oxane, morpholine, thiomorpholine, thiomorpholine-S-oxide, thiomorpholine-S,S-oxide, piperazine, pyran, pyridone, 3 -pyrroline, thiopyran, pyrone, tetrahydrofuran, tetrahydrothiophene, quinuclidine, tropane, 2-azaspiro[3.3]heptane, (lR,5S)-3- azabicyclo[3.2.1]octane, (ls,4s
- arene may mean an aromatic hydrocarbon ring.
- the arene may be a monocyclic arene or a polycyclic arene.
- the number of ring-forming carbons in the arene may be 5 or more and 30 or less, 5 or more and 20 or less, or 5 or more and 15 or less.
- Examples of the arene may include, but are not limited to, benzene, naphthalene, fluorene, anthracene, phenanthrene, bibenzene, terbenzene, quaterbenzene, quinquebenzene, sexibenzene, triphenylene, pyrene, benzofluoranthene, chrysene, and the like.
- the residue obtained by removing one hydrogen atom from "arene” is referred to as "aryl".
- heteroene may be a ring containing at least one of O, N, P, Si, and S as a heterogeneous element.
- the number of ring-forming carbons in the heteroarene may be 2 or more and 30 or less, or 2 or more and 20 or less.
- the heteroarene may be a monocyclic heteroarene or a polycyclic heteroarene.
- the polycyclic heteroarene may have, for example, a bicyclic or tricyclic structure.
- heteroarene may include thiophene, purine, pyrrole, pyrazole, imidazole, thiazole, oxazole, isothiazole, oxadiazole, triazole, pyridine, bipyridyl, triazine, acridyl, pyridazine, pyrazine, quinoline, quinazoline, quinoxaline, phenoxazine, phthalazine, pyrimidine, pyridopyrimidine, pyridopyrazine, pyrazinopyrazine, isoquinoline, indole, carbazole, imidazopyridazine, imidazopyridine, imidazopyrimidine, pyrazolopyrimidine, imidazopyrazine or pyrazolopyridine, N-aryl carb azole, N- heteroaryl carb azole, N-alkylcarbazole, benzoxazole,
- heteroarene may also include bicyclic heterocyclo-arene containing heteroarene fused to an arene ring or a cycloalkyl ring fused to heterocycloalkyl rings.
- heteroaryl the residue obtained by removing one hydrogen atom from the "heteroarene” is referred to as "heteroaryl”.
- the above-mentioned homogeneous or heterogeneous substituents may be substituted one or more at the same or different positions, and may be sequentially substituted.
- the meaning of "sequentially” means that in the formula, one substituent is substituted and then another substituent is successively substituted in the substituent, for example, a cycloalkyl group is substituted in the alkyl group after the alkyl group is substituted, and the When a carbonyl group is sequentially substituted for a cycloalkyl group, it can be indicated that the cycloalkyl group is sequentially substituted by naming it carbonylcycloalkylalkyl.
- connection radicals listed above do not specify the coupling direction, and the coupling direction is arbitrary.
- the radical L connected in can be -M-W-, where ring A and ring B can be connected in the same direction as the reading order from left to right to form and ring A and ring B can be connected in the opposite direction to the reading order from left to right to form
- stereoisomers e.g., enantiomers
- enantiomers mean compounds of the present invention or salts thereof that have the same chemical formula or molecular formula but are different in stereostructure. Each of these enantiomers and mixtures thereof are also included within the scope of the present invention.
- the straight solidline bond (-) connecting an asymmetric carbon atom may include a wedge-shaped solid-line bond or a wedge-shaped dashed-line bond indicating the absolute configuration of the stereocenter.
- stereoisomer and “optical isomer” are used interchangeably.
- the compound of Chemical Formula 1 of the present invention may exist in the form of a "pharmaceutically acceptable salt".
- a pharmaceutically acceptable salt As the salt, an acid addition salt formed by a pharmaceutically acceptable free acid is useful.
- pharmaceutically acceptable salt as used herein means any and all organic or inorganic acid addition salts of the compound represented by Chemical Formula 1 of which side effects caused by the salt do not reduce the beneficial efficacy of the compound at concentrations having an effective action that is relatively non-toxic and harmless to a patient.
- Acid addition salts are prepared by conventional methods, for example by dissolving the compound in an excess of aqueous acid solution and precipitating the salt using a water-miscible organic solvent such as methanol, ethanol, acetone or acetonitrile.
- a water-miscible organic solvent such as methanol, ethanol, acetone or acetonitrile.
- An acid or alcohol in an equimolar amount of the compound and water may be heated, and the mixture may then be evaporated to dryness, or the precipitated salt may be filtered off with suction.
- an organic acid and an inorganic acid may be used as the free acid, wherein the inorganic acid may be hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, or the like, and the organic acid may be methanesulfonic acid, p-toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, mandelic acid, propionic acid, citric acid, lactic acid, glycolic acid, gluconic acid, galacturonic acid, glutamic acid, glutaric acid, glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanillic acid, hydroiodic acid, or the like.
- the present invention is not limited thereto.
- the alkali metal salt or alkaline earth metal salt is obtained, for example, by dissolving a compound in an excess of alkali metal hydroxide or alkaline earth metal hydroxide solution, filtering the undissolved compound salt, and then evaporating and drying the filtrate.
- a sodium, potassium, or calcium salt as the metal salt, but the present invention is not limited thereto.
- the corresponding silver salt may be obtained by reacting an alkali metal or alkaline earth metal salt with a suitable silver salt (for example, silver nitrate).
- the pharmaceutically acceptable salt of the present invention includes salts of acidic or basic groups that may be present in the compound of Chemical Formula 1.
- the pharmaceutically acceptable salt may include sodium, calcium and potassium salts of hydroxyl groups, and the like, and as other pharmaceutically acceptable salts of amino groups, may include hydrobromide, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, dihydrogen phosphate, acetate, succinate, citrate, tartrate, lactate, mandelate, methanesulfonate(mesylate), and p-toluenesulfonate (tosylate) salts, and the like, and may be prepared by a method for preparing a salt known in the art.
- X is O. In certain embodiments, X is CH2.
- Ri is phenyl that may be substituted with -Ci-ealkyl, -Ci- ehydroxyalkyl, -Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo.
- Ri is 5- or 6-membered monocyclic heteroaryl that may be substituted with -Ci-ealkyl, -Ci-ehydroxyalkyl, - Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo.
- Ri is pyridinyl that may be substituted with -Ci-ealkyl, -Ci-ehydroxyalkyl, -Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo.
- Ri is -Ci-ealkyl that may be substituted with -Ci-ealkyl, -Ci- ehydroxyalkyl, -Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo.
- Ri is -Ci- ehaloalkyl.
- Ri is aryl in which at least one H of the aryl may be substituted with -Ci-ealkyl, -Ci-ehaloalkyl, or -halo. In certain embodiments, Ri is phenyl in which at least one H of the phenyl may be substituted with -Ci-ealkyl, -Ci-ehaloalkyl, or -halo. In certain embodiments, Ri is unsubstituted phenyl. In certain embodiments, Ri is phenyl in which at least one H of the phenyl is substituted with -halo (e.g., F). In certain embodiments, Ri is ortho monosubstituted phenyl.
- -halo e.g., F
- Ri is meta mono-substituted phenyl. In certain embodiments, Ri is para mono-substituted phenyl. In certain embodiments, Ri is di-substituted phenyl. In certain embodiments, Ri is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, Ri is - Ci-ehaloalkyl. In certain embodiments, Ri is -Ci-6 fluoroalkyl. In certain embodiments, Ri is fluoroethyl (e.g., -CH2CF3).
- R2 is H. In certain embodiments, R2 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R2 is Me. In certain embodiments, R2 is halo (e.g., F or Cl). In certain embodiments, R2 is F. In certain embodiments, R2 is H or F.
- R3 is -H. In certain embodiments, R3 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R3 is Me. In certain embodiments, R3 is halo (e.g., F or Cl). In certain embodiments, R3 is F.
- R4 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R4 is Me. In certain embodiments, R4 is halo (e.g., F or Cl). In certain embodiments, R4 is F.
- R5 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R5 is Me. In certain embodiments, R5 is -Ci-ehaloalkyl. In certain embodiments, R5 is -Ci-6 fluoroalkyl. In certain embodiments, R5 is fluoroethyl (e.g., -CH2CF3). In certain embodiments, R5 is cycloalkyl (e.g., cyclopropyl or cyclobutyl). In certain embodiments, R5 is cyclopropyl.
- R5 is -Ci-ealkyl-cycloalkyl (e.g., -Ci-ealkyl-cyclopropyl or -Ci-ealkyl-cyclobutyl). In certain embodiments, R5 is -CEE-cycloalkyl. In certain embodiments, R5 is Ci-ealkyl- cyclopropyl. In certain embodiments, R5 is -CFE-cyclopropyl.
- a compound of the present invention is a compound of Chemical Formula 1 (e.g., a compound of any one of Examples 1 to 15), or a stereoisomer thereof, or a pharmaceutically acceptable salt thereof.
- a compound of the present invention is a compound of Chemical Formula 1, or a pharmaceutically acceptable salt thereof.
- the present invention provides use of a compound represented by the following Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof.
- Chemical Formula 1 The Chemical Formula 1 is as defined above.
- the heteroaryl derivative compound represented by Chemical Formula 1 exhibits excellent inhibitory activity against RAF among kinases, and thus may be usefully employed for the treatment or prevention of RAF - related diseases, in particular, cancer.
- the heteroaryl derivative compound of the present invention exhibits excellent inhibitory activity against RAF mutations and can be usefully employed for treatment or prevention of carcinoma induced by RAF or RAF mutations.
- the cancer includes any cancer capable of exhibiting therapeutic or prophylactic efficacy due to inhibition of RAF activity, and may be a solid cancer or a hematologic cancer.
- the cancer may be one or more selected from the group consisting of pseudomyxoma, intrahepatic biliary tract cancer, hepatoblastoma, liver cancer, thyroid cancer, colon cancer, testicular cancer, myelodysplastic syndrome, glioblastoma, oral cancer, labial cancer, mycosis fungoides, acute myeloid leukemia, acute lymphocytic leukemia, basal cell cancer, ovarian epithelial cancer, ovarian germ cell cancer, male breast cancer, brain cancer, pituitary adenoma, multiple myeloma, gallbladder cancer, biliary tract cancer, colorectal cancer, chronic myelogenous leukemia, chronic lymphocytic leukemia, retinoblastoma, choroidal melanom
- the present invention provides a pharmaceutical composition comprising: a compound of the present invention; and optionally a pharmaceutically acceptable excipient.
- the present invention provides a kit comprising: a compound or pharmaceutical composition of the present invention; and instructions for using the compound or pharmaceutical composition.
- kits further comprises one or more containers.
- the present invention provides a pharmaceutical composition for treatment or prevention of RAF-related diseases containing the compound represented by Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient.
- the RAF-related disease may be cancer.
- the present invention provides a pharmaceutical composition for the treatment or prevention of cancer, comprising the compound, the stereoisomer thereof, or the pharmaceutically acceptable salt thereof, as an active ingredient.
- the types of cancer are the same as described above.
- the pharmaceutical composition of the present invention may further include one or more pharmaceutically acceptable carriers in addition to the compound represented by Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof for administration.
- the pharmaceutically acceptable carrier may be used in a mixture of saline, sterile water, ringer’s solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components, and other conventional additives such as antioxidants, buffers, and fungicides can be added as necessary.
- compositions for injection such as aqueous solutions, suspensions, and emulsions, pills, capsules, granules, or tablets.
- the composition of the present invention may be a patch agent, a liquid agent, a pill, a capsule, a granule, a tablet, a suppository, or the like.
- These formulations may be prepared by the usual method used in formulation in the art or by the method disclosed in the literature [Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA], and may be formulated into various formulations depending on each disease or ingredient.
- the pharmaceutical composition of the present invention may further include one or more active ingredients exhibiting the same or similar drug efficacy in addition to the compound represented by Chemical Formula 1 above, the stereoisomer thereof, or the pharmaceutically acceptable salt thereof.
- the present invention provides a method of treating a disease in a subject in need thereof, the method comprising administering to the subject in need thereof an effective amount of a compound or pharmaceutical composition of the present invention.
- the present invention provides a method of preventing a disease in a subject in need thereof, the method comprising administering to the subject in need thereof an effective amount of a compound or pharmaceutical composition of the present invention.
- the disease is a RAF-related disease. In certain embodiments, the disease is a disease induced by RAF mutations.
- the RAF is ARAF. In certain embodiments, the RAF is BRAF. In certain embodiments, the RAF is BRAF V600E. In certain embodiments, the RAF is RAFI.
- the disease is cancer.
- the disease is melanoma. In certain embodiments, the disease is colorectal cancer. In certain embodiments, the disease is thyroid cancer. In certain embodiments, the disease is ovarian cancer.
- the present invention provides a method of inhibiting the activity and/or production of RAF in a subject, cell, tissue, or biological sample, the method comprising administering to the subject or contacting the cell, tissue, or biological sample with an effective amount of a compound or pharmaceutical composition of the present invention.
- the subject is a human.
- the present invention provides an in vitro method of inhibiting the activity and/or production of RAF in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with an effective amount of a compound or pharmaceutical composition of the present invention.
- a method for treating or preventing RAF-related diseases comprising: administering to a subject in need thereof a therapeutically effective amount of the compound represented by Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof.
- the subject may be a mammal including a human.
- an “effective amount” of a compound or pharmaceutical composition refers to an amount of the compound or pharmaceutical composition sufficient to elicit a desired biological response.
- An effective amount may vary depending on such factors as the desired biological endpoint, the pharmacokinetics, the condition being treated, the mode of administration, and/or the age and health of the subject.
- the effective amount is a therapeutically effective amount (e.g., when a desired biological response is treatment of a disease).
- the effective amount is a prophylactically effective amount (e.g., when a desired biological response is prevention of a disease).
- therapeutically effective amount refers to an amount of the compound represented by Chemical Formula 1 that is effective for the treatment or prevention of RAF-related diseases. Specifically, “therapeutically effective amount” indicates an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level may be determined depending on factors including the subject type and severity, age, sex, type of disease, drug activity, drug sensitivity, administration time, administration route and excretion rate, treatment period, drugs used at the same time, and other factors well-known in medical fields.
- the pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or may be administered in combination with other therapeutic agents, and may be administered sequentially or simultaneously with commercially available therapeutic agents.
- the pharmaceutical composition of the present invention may be administered in a single dose or multiple doses. It is important to administer the minimum amount capable of obtaining the maximum effect without side effects in consideration of all of the above factors, and the amount may be readily determined by those skilled in the art.
- the dosage of the pharmaceutical composition of the present invention may be determined by a medical specialist according to various factors such as the patient's condition, age, sex, complications, and the like. Since the active ingredient of the pharmaceutical composition of the present invention has excellent safety, it may be used at a dose higher than the determined dosage.
- the present invention provides use of the compounds represented by Chemical Formula 1, the stereoisomer thereof, or the pharmaceutically acceptable salt thereof for use in preparation of a medicament to treat or prevent RAF-related diseases.
- the compounds represented by Chemical Formula 1 for preparing the medicament may be mixed with acceptable adjuvants, diluents, carriers, and the like, and may have a synergistic effect of active ingredients by being prepared as a complex formulation with other active agents.
- compositions and treatment methods of the present invention are applied equally except to the extent that they are inconsistent with each other.
- the heteroaryl derivative compounds of the present invention exhibits excellent inhibitory activity against RAF, and thus may be usefully employed for the treatment or prevention of RAF -related diseases. Best Mode
- Cell viability analysis was performed by culturing A375P cell line (Korea Cell Line Bank #80003) with a medium of Dulbecco' s Modified Eagles Medium(High Glucose)(Hyclone #SH30243.01) containing 10% fetal bovine serum(FBS) and 1% penicillin/streptomycin. More specifically, when performing the test, the cell line was aliquoted in a 96-well flat-bottom plate (corning #3903) at a concentration of 3,000 cells/well, respectively, and then cultured at 37°C for 24 hours under 5% CO2 conditions.
- the degree of cell proliferation inhibitory activity according to the treatment concentration of each compound was calculated based on the luminescence of the control cells not treated with the compound, and the concentration with 50% cell proliferation inhibitory activity was determined as the GIso(nM) value.
- GIso(nM) value was obtained using Prism (version 8.4.3 #GraphPad) software, and the results are shown in Table 2.
Abstract
The present invention relates to a heteroaryl derivative compound and a pharmaceutical composition comprising thereof. Specifically, the present invention relates to a heteroaryl derivative compounds having RAF inhibitory activity.
Description
HETEROARYL DERIVATIVE COMPOUNDS, AND PHARMACEUTICAL COMPOSITION COMPRISING THEREOF
RELATED APPLICATIONS
This application claims priority to KR10-2022-0058714, filed May 13, 2022, incorporated herein by reference in its entirety.
FIELD
The present invention relates to heteroaryl derivative compounds and a pharmaceutical composition comprising thereof. Specifically, the present invention relates to heteroaryl derivative compounds having RAF inhibitory activity.
BACKGROUND
The RAS/RAF/MEK/ERK protein kinase signaling pathway plays a very important role in the regulation of cellular function, and is specifically involved in cell proliferation, differentiation, survival, and angiogenesis (Biology of the Cell, 2001, 93, 53-62). In the signaling pathway, when guanosine triphosphate (GTP) is bound to the RAS protein, phosphorylation and activation of the RAF protein in the protoplasmic membrane proceeds. Subsequently, the activated RAF protein phosphorylates and activates the MEK protein, and the MEK protein phosphorylates and activates the ERK protein. Translocation of activated ERK from the cytoplasm to the nucleus results in regulation and phosphorylation of transcription factors such as Elk-1 and Myc.
RAF protooncogenes are serine/thr protein kinases that transmit signals from growth factor receptors activated in the cell membrane to transcription factors in the nucleus. The activation of the RAF protein is accompanied by phosphorylation of tyrosine, serine, and threonine residues of RAF protein, and direct phosphorylation by receptor tyrosine kinase or phosphorylation by protein phosphorylation enzymes controlled by these receptors is known as the mechanism of RAF activation. Among them, when controlled by a receptor, RAS is involved in the activation of RAF. Signals reaching RAF are then transferred to the nucleus via a signaling pathway leading to the RAF/MEK/ERK protein kinase. In this signaling pathway, a series of kinases are arranged into species to transmit signals, which play an essential role in cell growth and differentiation (Nature Rev. Mol. Cell. Biol., 2004, 5, 875-885), and the activity of RAF/MEK/ERK has been reported to be upregulated in a number of factor-dependent tumors.
As such, RAF acts as a major propagator of RAS function, providing a theoretical background for chemotherapy in the case of cancer with mutations or activations of RAS in inhibiting the action of this protein. RAF proteins have three isoforms of ARAF, BRAF, and CRAF (also known as RAF-1) with three functions(Biochim. Biophys. Acta., 2003, 1653, 25- 40), all three RAF genes are expressed in most tissues, and high expression of BRAF occurs in
neurocellular tissues and ARAF occurs in urinary reproductive tissues. Although each RAF family has a very similar amino acid sequence, biochemical activity and biological functionality are distinguished from each other (Exp. Cell. Res. 1999, 253, 34-46).
Studies have shown that BRAF is an important isoform protein related to cell proliferation and is an important target of oncogenic RAS. Abnormal mutations in the body have only been identified in BRAF cases, and are known to occur at a frequency of 30-60% in malignant skin melanoma (Nature, 2002, 417, 949-954), 30-50% in thyroid cancer, 5-20% in colon cancer, and 30% or less in ovarian cancer (Nature Rev. Mol. Cell Biology, 20045, 875 and 885). So far, more than 45 BRAF mutations have been known, but the most frequent mutation is that valine number 600 mutates with glutamic acid (V600E), which is observed in more than 90% of human cancers. This mutation is believed to increase the kinase activity of BRAF and transmit RAF/MEK/ERK signals to sub-signaling pathways that include structural activity of ERK as a result of RAS and growth factor receptor activation.
Accordingly, as a result of careful efforts to develop a new drug capable of inhibiting the activity of RAF, the present inventors confirmed that the compounds of the present invention have an anticancer effect by inhibiting the activity of RAF, thereby completing the present invention.
DISCLOSURE
Technical Problem
An object of the present invention is to provide a heteroaryl derivative having a novel structure, an stereoisomer thereof, or a pharmaceutically acceptable salt thereof.
Another object of the present invention is to provide a method for preparing the heteroaryl derivative compounds.
Still another object of the present invention is to provide a pharmaceutical use of the heteroaryl derivative compounds, and specifically, to a pharmaceutical composition for the treatment or prevention of RAF-related diseases comprising the heteroaryl derivative compounds as an active ingredient, use of the compounds for the treatment or prevention of RAF-related diseases, or a method for treating or preventing RAF-related diseases comprising administering the compounds. Technical Solution
In order to achieve the above-described objects, the present inventors made efforts to study, and as a result, found that the following heteroaryl derivative compounds represented by Chemical Formula 1 inhibited the proliferation of RAF-activated cells, and completed the present invention.
TECHNICAL SOLUTION
Preparation methods
Heteroaryl derivative compounds
The present invention provides a compound represented by following Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof:
(Chemical Formula 1) in the Chemical Formula 1, X is CH2, or O;
Y is CH or N;
Ri is -Ci-ealkyl, -Ci-ehaloalkyl, aryl or heteroaryl in which at least one H of the aryl or heteroaryl ring may be substituted with -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
R2 and R3 are each independently -H, -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
R4 is -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
R5 is -Ci-ealkyl, -Ci-ehaloalkyl, cycloalkyl, or -Ci-ealkyl-cycloalkyl.
According to an embodiment of the present invention, the compound represented by Chemical Formula 1 or a pharmaceutically acceptable salt thereof may be selected from the group consisting of the compounds of Examples 1 to 15 described in Table 1.
In the present invention, unless otherwise specified, the term "alkyl" may refer to a straight or branched chain acyclic, cyclic, or saturated hydrocarbon to which they are bonded. For example, "Ci-ealkyl" may indicate an alkyl containing 1 to 6 carbon atoms. As an example, acyclic alkyl may include, but is not limited to, methyl, ethyl, n-propyl, n-butyl, isopropyl, secbutyl, isobutyl, tert-butyl, or the like. Cyclic alkyl may be used interchangeably with
“cycloalkyl” as used herein, and as an example, may include, but is not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, or the like. In certain embodiments, the cycloalkyl is monocyclic. In certain embodiments, the cycloalkyl is 3- 7-membered.
In the present invention, “halo” or “halogen” may be F, Cl, Br, or I.
As used herein, "haloalkyl" may mean a straight or branched chain alkyl (hydrocarbon) having one or more halo-substituted carbon atoms as defined herein. Examples of the haloalkyl may include, but are not limited to, methyl, ethyl, propyl, isopropyl, isobutyl or n-butyl independently substituted with one or more halogens, such as F, Cl, Br, or I.
As used herein, "hydroxyalkyl" may indicate a straight or branched chain alkyl (hydrocarbon) having a carbon atom substituted with -hydroxy (-OH). Examples of the haloalkyl may include, but are not limited to, methyl, ethyl, propyl, isopropyl, isobutyl or n-butyl independently substituted with one or more -OH.
As used herein, "aminoalkyl" may mean a straight or branched chain alkyl (hydrocarbon) having a carbon atom substituted with amino (-NR'R"). Here, R' and R" may be each independently selected from the group consisting of hydrogen and Ci-ealkyl, and the selected R' and R" may be each independently substituted or unsubstituted.
In the present invention, "heterocycloalkyl" may mean a ring containing 1 to 5 heteroatoms selected from N, O and S as atoms forming the ring, and may be saturated or partially unsaturated. In certain embodiments, heterocycloalkyl is not aromatic. Here, when unsaturated, it may be referred to as a heterocycloalkene. Unless otherwise stated, heterocycloalkyl may be a single ring or a multiple ring such as a spiro ring, a bridged ring or a fused ring. In addition, "3- to 12-membered heterocycloalkyl" may indicate a heterocycloalkyl containing 3 to 12 atoms forming a ring. As an example, the heterocycloalkyl may include, but is not limited to, pyrrolidine, piperidine, imidazolidine, pyrazolidine, butyrolactam, valerolactam, imidazolidinone, hydantoin, dioxolane, phthalimide, piperidine, pyrimidin-2,4(lH,3H)-dione, 1,4-di oxane, morpholine, thiomorpholine, thiomorpholine-S-oxide, thiomorpholine-S,S-oxide, piperazine, pyran, pyridone, 3 -pyrroline, thiopyran, pyrone, tetrahydrofuran, tetrahydrothiophene, quinuclidine, tropane, 2-azaspiro[3.3]heptane, (lR,5S)-3- azabicyclo[3.2.1]octane, (ls,4s)-2-azabicyclo[2.2.2]octane, or (lR,4R)-2-oxa-5- azabicyclo[2.2.2]octane, and the like.
In the present invention, "arene" may mean an aromatic hydrocarbon ring. The arene may be a monocyclic arene or a polycyclic arene. The number of ring-forming carbons in the arene may be 5 or more and 30 or less, 5 or more and 20 or less, or 5 or more and 15 or less. Examples of the arene may include, but are not limited to, benzene, naphthalene, fluorene, anthracene, phenanthrene, bibenzene, terbenzene, quaterbenzene, quinquebenzene, sexibenzene,
triphenylene, pyrene, benzofluoranthene, chrysene, and the like. In the present specification, the residue obtained by removing one hydrogen atom from "arene" is referred to as "aryl".
In the present invention, "heteroarene" may be a ring containing at least one of O, N, P, Si, and S as a heterogeneous element. The number of ring-forming carbons in the heteroarene may be 2 or more and 30 or less, or 2 or more and 20 or less. The heteroarene may be a monocyclic heteroarene or a polycyclic heteroarene. The polycyclic heteroarene may have, for example, a bicyclic or tricyclic structure. Examples of the heteroarene may include thiophene, purine, pyrrole, pyrazole, imidazole, thiazole, oxazole, isothiazole, oxadiazole, triazole, pyridine, bipyridyl, triazine, acridyl, pyridazine, pyrazine, quinoline, quinazoline, quinoxaline, phenoxazine, phthalazine, pyrimidine, pyridopyrimidine, pyridopyrazine, pyrazinopyrazine, isoquinoline, indole, carbazole, imidazopyridazine, imidazopyridine, imidazopyrimidine, pyrazolopyrimidine, imidazopyrazine or pyrazolopyridine, N-aryl carb azole, N- heteroaryl carb azole, N-alkylcarbazole, benzoxazole, benzoimidazole, benzothiazole, benzocarbazole, benzothiophene, dibenzothiophene, thienothiophene, benzofuran, phenanthroline, isoxazole, oxadiazole, thiadiazole, benzothiazole, tetrazole, phenothiazine, dibenzosilole, dibenzofuran, and the like, but are not limited thereto. In an embodiment of the present invention, heteroarene may also include bicyclic heterocyclo-arene containing heteroarene fused to an arene ring or a cycloalkyl ring fused to heterocycloalkyl rings. In the present specification, the residue obtained by removing one hydrogen atom from the "heteroarene" is referred to as "heteroaryl".
The above-mentioned homogeneous or heterogeneous substituents may be substituted one or more at the same or different positions, and may be sequentially substituted. The meaning of "sequentially" means that in the formula, one substituent is substituted and then another substituent is successively substituted in the substituent, for example, a cycloalkyl group is substituted in the alkyl group after the alkyl group is substituted, and the When a carbonyl group is sequentially substituted for a cycloalkyl group, it can be indicated that the cycloalkyl group is sequentially substituted by naming it carbonylcycloalkylalkyl.
In addition, the connection radicals listed above do not specify the coupling direction, and the coupling direction is arbitrary. For example, the radical L connected in
can be -M-W-, where ring A and ring B can be connected in the same direction as the reading order from left to right to form
and ring A and ring B can be connected in the opposite direction to the reading order from left to right to form
In the present invention, the term "stereoisomers (e.g., enantiomers)" mean compounds of the present invention or salts thereof that have the same chemical formula or molecular formula but are different in stereostructure. Each of these enantiomers and mixtures thereof are also included within the scope of the present invention. Unless otherwise specified, the straight solidline bond (-) connecting an asymmetric carbon atom may include a wedge-shaped solid-line bond
or a wedge-shaped dashed-line bond indicating the absolute configuration of the stereocenter. The term “stereoisomer” and “optical isomer” are used interchangeably.
The compound of Chemical Formula 1 of the present invention may exist in the form of a "pharmaceutically acceptable salt". As the salt, an acid addition salt formed by a pharmaceutically acceptable free acid is useful. The term "pharmaceutically acceptable salt" as used herein means any and all organic or inorganic acid addition salts of the compound represented by Chemical Formula 1 of which side effects caused by the salt do not reduce the beneficial efficacy of the compound at concentrations having an effective action that is relatively non-toxic and harmless to a patient.
Acid addition salts are prepared by conventional methods, for example by dissolving the compound in an excess of aqueous acid solution and precipitating the salt using a water-miscible organic solvent such as methanol, ethanol, acetone or acetonitrile. An acid or alcohol in an equimolar amount of the compound and water may be heated, and the mixture may then be evaporated to dryness, or the precipitated salt may be filtered off with suction.
Here, an organic acid and an inorganic acid may be used as the free acid, wherein the inorganic acid may be hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, or the like, and the organic acid may be methanesulfonic acid, p-toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, mandelic acid, propionic acid, citric acid, lactic acid, glycolic acid, gluconic acid, galacturonic acid, glutamic acid, glutaric acid, glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanillic acid, hydroiodic acid, or the like. However, the present invention is not limited thereto.
In addition, it is possible to prepare a pharmaceutically acceptable metal salt using a base. The alkali metal salt or alkaline earth metal salt is obtained, for example, by dissolving a compound in an excess of alkali metal hydroxide or alkaline earth metal hydroxide solution, filtering the undissolved compound salt, and then evaporating and drying the filtrate. Here, it is pharmaceutically suitable to prepare a sodium, potassium, or calcium salt as the metal salt, but
the present invention is not limited thereto. Further, the corresponding silver salt may be obtained by reacting an alkali metal or alkaline earth metal salt with a suitable silver salt (for example, silver nitrate).
Unless otherwise indicated, the pharmaceutically acceptable salt of the present invention includes salts of acidic or basic groups that may be present in the compound of Chemical Formula 1. For example, the pharmaceutically acceptable salt may include sodium, calcium and potassium salts of hydroxyl groups, and the like, and as other pharmaceutically acceptable salts of amino groups, may include hydrobromide, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, dihydrogen phosphate, acetate, succinate, citrate, tartrate, lactate, mandelate, methanesulfonate(mesylate), and p-toluenesulfonate (tosylate) salts, and the like, and may be prepared by a method for preparing a salt known in the art.
In certain embodiments, X is O. In certain embodiments, X is CH2.
In certain embodiments, Ri is phenyl that may be substituted with -Ci-ealkyl, -Ci- ehydroxyalkyl, -Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo. In certain embodiments, Ri is 5- or 6-membered monocyclic heteroaryl that may be substituted with -Ci-ealkyl, -Ci-ehydroxyalkyl, - Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo. In certain embodiments, Ri is pyridinyl that may be substituted with -Ci-ealkyl, -Ci-ehydroxyalkyl, -Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo. In certain embodiments, Ri is -Ci-ealkyl that may be substituted with -Ci-ealkyl, -Ci- ehydroxyalkyl, -Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo. In certain embodiments, Ri is -Ci- ehaloalkyl.
In certain embodiments, Ri is aryl in which at least one H of the aryl may be substituted with -Ci-ealkyl, -Ci-ehaloalkyl, or -halo. In certain embodiments, Ri is phenyl in which at least
one H of the phenyl may be substituted with -Ci-ealkyl, -Ci-ehaloalkyl, or -halo. In certain embodiments, Ri is unsubstituted phenyl. In certain embodiments, Ri is phenyl in which at least one H of the phenyl is substituted with -halo (e.g., F). In certain embodiments, Ri is ortho monosubstituted phenyl. In certain embodiments, Ri is meta mono-substituted phenyl. In certain embodiments, Ri is para mono-substituted phenyl. In certain embodiments, Ri is di-substituted phenyl. In certain embodiments, Ri is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, Ri is - Ci-ehaloalkyl. In certain embodiments, Ri is -Ci-6 fluoroalkyl. In certain embodiments, Ri is fluoroethyl (e.g., -CH2CF3).
In certain embodiments, R2 is H. In certain embodiments, R2 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R2 is Me. In certain embodiments, R2 is halo (e.g., F or Cl). In certain embodiments, R2 is F. In certain embodiments, R2 is H or F.
In certain embodiments, R3 is -H. In certain embodiments, R3 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R3 is Me. In certain embodiments, R3 is halo (e.g., F or Cl). In certain embodiments, R3 is F.
In certain embodiments, R4 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R4 is Me. In certain embodiments, R4 is halo (e.g., F or Cl). In certain embodiments, R4 is F.
In certain embodiments, R5 is -Ci-ealkyl (e.g., Me or Et). In certain embodiments, R5 is Me. In certain embodiments, R5 is -Ci-ehaloalkyl. In certain embodiments, R5 is -Ci-6 fluoroalkyl. In certain embodiments, R5 is fluoroethyl (e.g., -CH2CF3). In certain embodiments, R5 is cycloalkyl (e.g., cyclopropyl or cyclobutyl). In certain embodiments, R5 is cyclopropyl. In certain embodiments, R5 is -Ci-ealkyl-cycloalkyl (e.g., -Ci-ealkyl-cyclopropyl or -Ci-ealkyl-cyclobutyl). In certain embodiments, R5 is -CEE-cycloalkyl. In certain embodiments, R5 is Ci-ealkyl- cyclopropyl. In certain embodiments, R5 is -CFE-cyclopropyl.
In certain embodiments, a compound of the present invention is a compound of Chemical Formula 1 (e.g., a compound of any one of Examples 1 to 15), or a stereoisomer thereof, or a pharmaceutically acceptable salt thereof. In certain embodiments, a compound of the present invention is a compound of Chemical Formula 1, or a pharmaceutically acceptable salt thereof.
Use of Heteroaryl Derivative Compounds
The present invention provides use of a compound represented by the following Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof.
According to an embodiment of the present invention, the heteroaryl derivative compound represented by Chemical Formula 1 exhibits excellent inhibitory activity against RAF among kinases, and thus may be usefully employed for the treatment or prevention of RAF - related diseases, in particular, cancer. Specifically, the heteroaryl derivative compound of the present invention exhibits excellent inhibitory activity against RAF mutations and can be usefully employed for treatment or prevention of carcinoma induced by RAF or RAF mutations.
In the present invention, the cancer includes any cancer capable of exhibiting therapeutic or prophylactic efficacy due to inhibition of RAF activity, and may be a solid cancer or a hematologic cancer. For example, the cancer may be one or more selected from the group consisting of pseudomyxoma, intrahepatic biliary tract cancer, hepatoblastoma, liver cancer, thyroid cancer, colon cancer, testicular cancer, myelodysplastic syndrome, glioblastoma, oral cancer, labial cancer, mycosis fungoides, acute myeloid leukemia, acute lymphocytic leukemia, basal cell cancer, ovarian epithelial cancer, ovarian germ cell cancer, male breast cancer, brain cancer, pituitary adenoma, multiple myeloma, gallbladder cancer, biliary tract cancer, colorectal cancer, chronic myelogenous leukemia, chronic lymphocytic leukemia, retinoblastoma, choroidal melanoma, ampulla of vater cancer, bladder cancer, peritoneal cancer, parathyroid cancer, adrenal cancer, sinus cancer, non-small cell lung cancer, tongue cancer, astrocytoma, small cell lung cancer, pediatric brain cancer, pediatric lymphoma, pediatric leukemia, small intestine cancer, meningioma, esophageal cancer, glioma, renal pelvic cancer, kidney cancer, heart cancer, duodenal cancer, malignant soft tissue cancer, malignant bone cancer, malignant lymphoma, malignant mesothelioma, malignant melanoma, eye cancer, vulvar cancer, ureter cancer, urethral cancer, cancer of unknown primary site, gastric lymphoma, gastric cancer, gastric carcinoma, gastrointestinal stromal cancer, Wilms cancer, breast cancer, sarcoma, penile cancer, pharyngeal cancer, gestational choriocarcinoma, cervical cancer, endometrial cancer, uterine sarcoma, prostate cancer, metastatic bone cancer, metastatic brain cancer, mediastinal cancer, rectal cancer, rectal carcinoma, vaginal cancer, spinal cord cancer, acoustic tumor, pancreatic cancer, salivary gland cancer, Kaposi's sarcoma, Paget's disease, tonsil cancer, squamous cell carcinoma, lung adenocarcinoma, lung cancer, lung squamous cell carcinoma, skin cancer, anal cancer, rhabdomyosarcoma, laryngeal cancer, pleural cancer, blood cancer, and thymus cancer, but is not limited thereto. The cancer includes not only primary cancer but also metastatic cancer.
In another aspect, the present invention provides a pharmaceutical composition comprising: a compound of the present invention; and optionally a pharmaceutically acceptable excipient.
In another aspect, the present invention provides a kit comprising: a compound or pharmaceutical composition of the present invention; and instructions for using the compound or pharmaceutical composition.
In certain embodiments, the instructions are information as required by a regulatory agency such as the U.S. Food and Drug Administration (FDA) or European Medicines Agency (EMA). In certain embodiments, a kit further comprises one or more containers.
According to an embodiment of the present invention, the present invention provides a pharmaceutical composition for treatment or prevention of RAF-related diseases containing the compound represented by Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof as an active ingredient. Specifically, the RAF-related disease may be cancer. In certain embodiments, the present invention provides a pharmaceutical composition for the treatment or prevention of cancer, comprising the compound, the stereoisomer thereof, or the pharmaceutically acceptable salt thereof, as an active ingredient. The types of cancer are the same as described above.
The pharmaceutical composition of the present invention may further include one or more pharmaceutically acceptable carriers in addition to the compound represented by Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof for administration. The pharmaceutically acceptable carrier may be used in a mixture of saline, sterile water, ringer’s solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components, and other conventional additives such as antioxidants, buffers, and fungicides can be added as necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants can be added to formulations for injection, such as aqueous solutions, suspensions, and emulsions, pills, capsules, granules, or tablets. Accordingly, the composition of the present invention may be a patch agent, a liquid agent, a pill, a capsule, a granule, a tablet, a suppository, or the like. These formulations may be prepared by the usual method used in formulation in the art or by the method disclosed in the literature [Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA], and may be formulated into various formulations depending on each disease or ingredient.
The pharmaceutical composition of the present invention may further include one or more active ingredients exhibiting the same or similar drug efficacy in addition to the compound represented by Chemical Formula 1 above, the stereoisomer thereof, or the pharmaceutically acceptable salt thereof.
In another aspect, the present invention provides a method of treating a disease in a subject in need thereof, the method comprising administering to the subject in need thereof an effective amount of a compound or pharmaceutical composition of the present invention.
In another aspect, the present invention provides a method of preventing a disease in a subject in need thereof, the method comprising administering to the subject in need thereof an effective amount of a compound or pharmaceutical composition of the present invention.
In certain embodiments, the disease is a RAF-related disease. In certain embodiments, the disease is a disease induced by RAF mutations.
In certain embodiments, the RAF is ARAF. In certain embodiments, the RAF is BRAF. In certain embodiments, the RAF is BRAF V600E. In certain embodiments, the RAF is RAFI.
In certain embodiments, the disease is cancer.
In certain embodiments, the disease is melanoma. In certain embodiments, the disease is colorectal cancer. In certain embodiments, the disease is thyroid cancer. In certain embodiments, the disease is ovarian cancer.
In another aspect, the present invention provides a method of inhibiting the activity and/or production of RAF in a subject, cell, tissue, or biological sample, the method comprising administering to the subject or contacting the cell, tissue, or biological sample with an effective amount of a compound or pharmaceutical composition of the present invention.
In certain embodiments, the subject is a human.
In another aspect, the present invention provides an in vitro method of inhibiting the activity and/or production of RAF in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with an effective amount of a compound or pharmaceutical composition of the present invention.
Further, according to an embodiment of the present invention, there is provided a method for treating or preventing RAF-related diseases, comprising: administering to a subject in need thereof a therapeutically effective amount of the compound represented by Chemical Formula 1, a stereoisomer thereof, or a pharmaceutically acceptable salt thereof. The subject may be a mammal including a human.
An “effective amount” of a compound or pharmaceutical composition refers to an amount of the compound or pharmaceutical composition sufficient to elicit a desired biological response. An effective amount may vary depending on such factors as the desired biological endpoint, the pharmacokinetics, the condition being treated, the mode of administration, and/or the age and health of the subject. In certain embodiments, the effective amount is a therapeutically effective amount (e.g., when a desired biological response is treatment of a disease). In certain embodiments, the effective amount is a prophylactically effective amount (e.g., when a desired biological response is prevention of a disease).
The term "therapeutically effective amount" as used herein refers to an amount of the compound represented by Chemical Formula 1 that is effective for the treatment or prevention of
RAF-related diseases. Specifically, "therapeutically effective amount" indicates an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level may be determined depending on factors including the subject type and severity, age, sex, type of disease, drug activity, drug sensitivity, administration time, administration route and excretion rate, treatment period, drugs used at the same time, and other factors well-known in medical fields. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or may be administered in combination with other therapeutic agents, and may be administered sequentially or simultaneously with commercially available therapeutic agents. In addition, the pharmaceutical composition of the present invention may be administered in a single dose or multiple doses. It is important to administer the minimum amount capable of obtaining the maximum effect without side effects in consideration of all of the above factors, and the amount may be readily determined by those skilled in the art. The dosage of the pharmaceutical composition of the present invention may be determined by a medical specialist according to various factors such as the patient's condition, age, sex, complications, and the like. Since the active ingredient of the pharmaceutical composition of the present invention has excellent safety, it may be used at a dose higher than the determined dosage.
Further, according to an embodiment of the present invention, the present invention provides use of the compounds represented by Chemical Formula 1, the stereoisomer thereof, or the pharmaceutically acceptable salt thereof for use in preparation of a medicament to treat or prevent RAF-related diseases. The compounds represented by Chemical Formula 1 for preparing the medicament may be mixed with acceptable adjuvants, diluents, carriers, and the like, and may have a synergistic effect of active ingredients by being prepared as a complex formulation with other active agents.
Matters mentioned in the uses, compositions and treatment methods of the present invention are applied equally except to the extent that they are inconsistent with each other.
An embodiment of the present invention may be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below. In addition, embodiments of the present invention are provided to more fully explain the present invention to those with average knowledge in the art. Furthermore, "includes" a component throughout the specification means that it may include other components, not excluding other components unless otherwise opposed.
Advantageous Effects
The heteroaryl derivative compounds of the present invention exhibits excellent inhibitory activity against RAF, and thus may be usefully employed for the treatment or
prevention of RAF -related diseases. Best Mode
Hereinafter, the present invention will be described in more detail through Examples and Experimental Examples. However, the following Examples and Experimental Examples are merely presented to illustrate the present invention, and the scope of the present invention is not limited thereto.
EXAMPLES The compounds of Examples 1 to 15 were prepared in a manner similar to Reaction
Scheme 1. The compound name, chemical structural formula, NMR and LCMS analysis results of each Example compound are summarized in Table 1 below. Table 1
Experimental Example 1: Evaluation of cell proliferation inhibition activity in A375P cells of certain compounds according to the present invention
The following experiments were performed to evaluate the cell proliferation inhibitory activity of the compounds according to the present invention. Cell viability analysis was performed by culturing A375P cell line (Korea Cell Line Bank #80003) with a medium of Dulbecco' s Modified Eagles Medium(High Glucose)(Hyclone #SH30243.01) containing 10% fetal bovine serum(FBS) and 1% penicillin/streptomycin. More specifically, when performing the test, the cell line was aliquoted in a 96-well flat-bottom plate (corning #3903) at a concentration of 3,000 cells/well, respectively, and then cultured at 37°C for 24 hours under 5% CO2 conditions. Compounds in each well were treated with 11 concentrations by giving a 3 -fold concentration gradient to the highest concentration of 10 pM, and dimethylsulfoxide (DMSO) was treated at the same concentration of 0.5% (v/v) as in the compound treatment as each control group. The compound treated cells were incubated for 72 hours. To check the degree of cell viability, 100 pl of Cell Titer-Gio (Promega #G7573) was added to the culture medium of each cultured cell, and then incubated for another 10 minutes at room temperature, and then luminescence was measured using a microplate reader. The degree of cell proliferation inhibitory activity according to the treatment concentration of each compound was calculated based on the luminescence of the control cells not treated with the compound, and the concentration with 50% cell proliferation inhibitory activity was determined as the GIso(nM) value. GIso(nM) value was obtained using Prism (version 8.4.3 #GraphPad) software, and the results are shown in Table 2.
Experimental Example 2: Evaluation of cell proliferation inhibition activity in HCT116 cell of certain compounds according to the present invention
The following experiments were performed to evaluate the cell proliferation inhibitory activity of the compounds according to the present invention. Cell viability analysis was performed by culturing HCT116 cell line (Korea Cell Line Bank #10247) with a medium of McCoy's 5A (Modified) Medium (Gibco #16600082) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. More specifically, when performing the test, the cell line was aliquoted in a 96-well flat-bottom plate (coming #3903) at a concentration of 2,000 cells/well, respectively, and then cultured at 37°C for 24 hours under 5% CO2 conditions. Compounds in each well were treated with 11 concentrations by giving a 3-fold concentration gradient to the highest concentration of 10 pM, and dimethylsulfoxide (DMSO) was treated at the same concentration of 0.5% (v/v) as in the compound treatment as each control group. The compound treated cells were incubated for 72 hours. To check the degree of cell viability, 100 pl of Cell Titer-Gio (Promega #G7573) was added to the culture medium of each cultured cell, and then incubated for another 10 minutes at room temperature, and then luminescence was measured using a microplate reader. The degree of cell proliferation inhibitory activity according to the treatment concentration of each compound was calculated based on the luminescence of the control cells not treated with the compound, and the concentration with 50% cell proliferation inhibitory activity was determined as the GIso(nM) value. GIso(nM) value was obtained using Prism (version 8.4.3 #GraphPad) software, and the results are shown in Table 2.
Claims
1. A compound represented by the following Chemical Formula 1, an optical isomer thereof, or a pharmaceutically acceptable salt thereof:
(Chemical Formula 1) in the Chemical Formula 1,
X is CH2, or O;
Y is CH or N;
Ri is -Ci-ealkyl, -Ci-ehaloalkyl, aryl or heteroaryl in which at least one H of the aryl or heteroaryl ring may be substituted with -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
R2 and R3 are each independently -H, -Ci-ealkyl, -Ci-ehaloalkyl, or -halo;
R4 is -Ci-ealkyl, -Ci-ehaloalkyl, or -halo; and
R5 is -Ci-ealkyl, -Ci-ehaloalkyl, cycloalkyl, or -Ci-ealkyl-cycloalkyl.
2. The compound of claim 1, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein X is O.
3. The compound of claim 1, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein X is CH2.
4. The compound of any one of claims 1-3, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Ri is phenyl that may be substituted with -Ci- ealkyl, -Ci-ehydroxyalkyl, -Ci-eaminoalkyl, -Ci-ehaloalkyl, -CN, or -halo.
5. The compound of claim 4, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Ri is unsubstituted phenyl or phenyl in which at least one H of the phenyl is substituted with -halo.
6. The compound of claim 5, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Ri is phenyl in which at least one -H of the phenyl is substituted with -F.
7. The compound of any one of claims 1-3, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Ri is -Ci-ealkyl or -Ci-ehaloalkyl.
8. The compound of claim 7, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Ri is -Ci-6 fluoroalkyl.
9. The compound of claim 8, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Ri is -CH2CF3.
13. The compound of any one of claims 1-12, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Y is CH.
14. The compound of any one of claims 1-12, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein Y is N.
15. The compound of any one of claims 1-14, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R2 is -H.
16. The compound of any one of claims 1-14, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R2 is -halo.
17. The compound of claim 16, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R2 is -F.
18. The compound of any one of claims 1-17, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R3 is -Ci-ealkyl.
19. The compound of claim 18, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R3 is Me.
20. The compound of any one of claims 1-19, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R4 is -halo or -Ci-ealkyl.
21. The compound of claim 20, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R4 is -F or Me.
22. The compound of any one of claims 1-21, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R5 is -Ci-ealkyl or -Ci-ehaloalkyl.
23. The compound of claim 22, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R5 is Me or -CH2CF3.
24. The compound of any one of claims 1-21, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R5 is cycloalkyl or -Ci-ealkyl-cycloalkyl.
25. The compound of claim 24, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, wherein R5 is cyclopropyl or -CFF-cyclopropyl.
26. The compound of claim 1, or an optical isomer thereof, or a pharmaceutically acceptable salt thereof, wherein the compound is of the formula:
2 27. The compound of any one of claims 1-26, or the pharmaceutically acceptable salt thereof.
4 28. A pharmaceutical composition comprising:
a compound of any one of claims 1-26, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof; and optionally a pharmaceutically acceptable excipient.
29. The pharmaceutical composition of claim 28 further comprising one or more active ingredients.
30. A kit compri sing : a compound of any one of claims 1-26, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, or a pharmaceutical composition of any one of claims 28-29; and instructions for using the compound, optical isomer, pharmaceutically acceptable salt, or pharmaceutical composition.
31. A method of treating a disease in a subject in need thereof, the method comprising administering to the subject in need thereof an effective amount of a compound of any one of claims 1-26, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, or a pharmaceutical composition of any one of claims 28-29.
32. A method of preventing a disease in a subject in need thereof, the method comprising administering to the subject in need thereof an effective amount of a compound of any one of claims 1-26, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, or a pharmaceutical composition of any one of claims 28-29.
33. The method of any one of claims 31-32, wherein the disease is a RAF-related disease.
34. The method of any one of claims 31-33, wherein the disease is a disease induced by RAF mutations.
35. The method of any one of claims 33-34, wherein the RAF is BRAF.
36. The method of any one of claims 31-35, wherein the disease is cancer.
37. The method of any one of claims 31-35, wherein the disease is melanoma.
38. The method of any one of claims 31-35, wherein the disease is colorectal cancer.
39. The method of any one of claims 31-35, wherein the disease is thyroid cancer.
40. The method of any one of claims 31-35, wherein the disease is ovarian cancer.
41. A method of inhibiting the activity and/or production of RAF in a subject, cell, tissue, or biological sample, the method comprising administering to the subject or contacting the cell, tissue, or biological sample with an effective amount of a compound of any one of claims 1-26, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, or a pharmaceutical composition of any one of claims 28-29.
42. The method of any one of claims 31-41, wherein the subject is a human.
43. An in vitro method of inhibiting the activity and/or production of RAF in a cell, tissue, or biological sample, the method comprising contacting the cell, tissue, or biological sample with an effective amount of a compound of any one of claims 1-26, or the optical isomer thereof, or the pharmaceutically acceptable salt thereof, or a pharmaceutical composition of any one of claims 28-29.
44. The method of any one of claims 41-43, wherein the RAF is BRAF.
45. A pharmaceutical composition for the treatment or prevention of cancer, comprising the compound, the optical isomer thereof, or the pharmaceutically acceptable salt thereof according to any one of claims 1-26 as an active ingredient.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20220058714 | 2022-05-13 | ||
KR10-2022-0058714 | 2022-05-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2023218241A1 true WO2023218241A1 (en) | 2023-11-16 |
Family
ID=88729825
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2023/000256 WO2023218241A1 (en) | 2022-05-13 | 2023-05-12 | Heteroaryl derivative compounds, and pharmaceutical composition comprising thereof |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2023218241A1 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005113548A1 (en) * | 2004-05-20 | 2005-12-01 | Sugen, Inc. | Thiophene heteroaryl amines |
WO2007117607A2 (en) * | 2006-04-06 | 2007-10-18 | Novartis Ag | Quinazolines for pdk1 inhibition |
WO2008079988A2 (en) * | 2006-12-22 | 2008-07-03 | Novartis Ag | Quinazolines for pdk1 inhibition |
WO2009153313A1 (en) * | 2008-06-20 | 2009-12-23 | Novartis Ag | 2 -arylaminoquinazolines for treating proliferative diseases |
WO2010019637A1 (en) * | 2008-08-12 | 2010-02-18 | Smithkline Beecham Corporation | Chemical compounds |
-
2023
- 2023-05-12 WO PCT/IB2023/000256 patent/WO2023218241A1/en unknown
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005113548A1 (en) * | 2004-05-20 | 2005-12-01 | Sugen, Inc. | Thiophene heteroaryl amines |
WO2007117607A2 (en) * | 2006-04-06 | 2007-10-18 | Novartis Ag | Quinazolines for pdk1 inhibition |
WO2008079988A2 (en) * | 2006-12-22 | 2008-07-03 | Novartis Ag | Quinazolines for pdk1 inhibition |
WO2009153313A1 (en) * | 2008-06-20 | 2009-12-23 | Novartis Ag | 2 -arylaminoquinazolines for treating proliferative diseases |
WO2010019637A1 (en) * | 2008-08-12 | 2010-02-18 | Smithkline Beecham Corporation | Chemical compounds |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111646995B (en) | 4-amino-pyrimidoazenitrogen heterocycle-phenylurea derivative and preparation method and application thereof | |
KR20200100717A (en) | Biaryl derivatives, their synthesis and their pharmaceutical use | |
KR102575246B1 (en) | Phenyl-2-hydroxy-acetylamino-2-methyl-phenyl compound | |
JP2020510642A (en) | o-Aminoheteroarylalkynyl group-containing compound and its production method and use | |
JP6088542B2 (en) | Substituted imidazopyrazines as Akt kinase inhibitors | |
BR112014023460B1 (en) | COMPOUND, PHARMACEUTICAL FORMULATION, USE OF A COMPOUND | |
CA3178415A1 (en) | Salt and crystal forms of 4-amino-5-(6-(4-methylpiperazin-1-yl)-1h-benzo[d]imidazol-2-yl)thieno[2,3-b]pyridin-6(7h)-one | |
JP6979595B2 (en) | Aminothiazole compounds as protein kinase inhibitors | |
JP2021121599A (en) | Histone demethylase inhibitors | |
JP6666458B2 (en) | Substituted amino 6-membered nitrogen-containing heterocyclic compounds and their production and use | |
WO2023218241A1 (en) | Heteroaryl derivative compounds, and pharmaceutical composition comprising thereof | |
WO2023218245A1 (en) | Heteroaryl derivative compounds, and pharmaceutical composition comprising thereof | |
CN114380823B (en) | Imidazole-2-methylamine derivative and medical application thereof | |
TW202333667A (en) | Pyrimidine or pyridine derivative and pharmaceutical use thereof | |
KR20220097305A (en) | Heteroaryl derivative compounds, and uses thereof | |
WO2023187471A1 (en) | Heteroaryl derivative compounds, and uses thereof | |
KR20240002289A (en) | Heteroaryl derivative compounds, and uses thereof | |
CN110698471A (en) | ASK1 inhibitor and application thereof | |
KR20230142000A (en) | Isoxazolidine derivative compounds, and uses thereof | |
CA3223059A1 (en) | Pyrimidine-4,6-diamine derivative, a preparation method therefor, and a pharmaceutical application thereof | |
KR20240041340A (en) | Pyrrolopyrimidine derivative compounds and uses thereof | |
KR20230056618A (en) | Benzothiophene derivatives as STAT3 inhibitor, and uses thereof | |
KR20230038125A (en) | Heteroaryl derivatives as STAT3 inhibitor, and uses thereof | |
CN113912603A (en) | Isochrysine analogue, preparation method and application of isochrysine analogue from ciprofloxacin | |
CN117756781A (en) | Indole histone deacetylase family inhibitor with anti-tumor effect |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 23803095 Country of ref document: EP Kind code of ref document: A1 |